Skin care substance and skin care product

Abstract

Provided are a skin care active substance and a skin care product. The skin care active substance comprises, in part by weight: 0.31-13 parts of an antioxidant and 0.1-10 parts of a humectant. The antioxidant comprises, in part by weight, 0.1-5 parts of ascorbyl glucoside, 0.1-2 parts of tocopheryl acetate, 0.01-1 part of silymarin, and 0.1-5 parts of a Calamus draco extract. The humectant comprises at least one of sodium hyaluronate crosspolymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and sodium acetylated hyaluronate. Through the design of the above ingredients and the proportions, the formed synergistic active substances can exert a mild and efficient antioxidant effect, protect the skin barrier effectively, and keep the health of the skin, with good safety.

Description

Skin care actives, skin care products

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application claims priority based on Chinese patent application 202410115267.9 filed on January 25, 2024, and all of its contents are incorporated herein by reference. Technical Field

[0003] The present application relates to the technical field of skin care, and in particular to skin care active ingredients and skin care products. Background Art

[0004] Human skin is the body's largest organ and its first defense against external aggression. When exposed to internal or external environmental factors, excessive amounts of reactive oxygen species (ROS) trigger a series of biological reactions known as oxidative stress, which contributes to skin aging.

[0005] Oxidative stress in the human body not only changes the structure and function of proteins, lipids, DNA, etc. at the molecular level, but also causes cell apoptosis and degradation of the extracellular matrix, ultimately leading to inflammation, aging, etc.

[0006] Summary of the Invention

[0007] The present application provides a skin care active ingredient and a skin care product, which have antioxidant effects, effectively protect the skin barrier, and maintain skin health.

[0008] To solve the above technical problems, the first aspect of the present application provides a skin care active ingredient, which includes 0.31-13 parts by weight of an antioxidant and 0.1-10 parts by weight of a moisturizer in a weight ratio; wherein the antioxidant includes 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, and 0.1-5 parts by weight of unicorn extract; and the moisturizer includes at least one of sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate.

[0009] In one embodiment, the skin care active ingredients include, by weight, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2); or,

[0010] The skin care active ingredient comprises, by weight, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate; or

[0011] The skin care active ingredients include, by weight, 0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1; or,

[0012] The skin care active ingredients include, by weight, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1; or

[0013] The skin care active ingredients include, by weight, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1; or

[0014] The skin care active ingredient comprises, by weight, 0.1-10 parts by weight of sodium hyaluronate.

[0015] In one embodiment, the skin care active ingredient is composed of the following components in a weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2).

[0016] In one embodiment, the skin care active ingredient is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, and 0.1-10 parts by weight of hydrolyzed sodium hyaluronate.

[0017] In one embodiment, the skin care active is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate and hydrolyzed sodium hyaluronate, and the weight ratio of the sodium hyaluronate, the acetylated sodium hyaluronate and the hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1.

[0018] In one embodiment, the skin care active is composed of the following components in a weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the acetylated sodium hyaluronate and the hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1; or,

[0019] The skin care active ingredient is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1; or,

[0020] The skin care active ingredient is composed of the following components in the following weight ratios: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, and 0.1-10 parts by weight of sodium hyaluronate.

[0021] In order to solve the above technical problems, the second aspect of the present application provides a skin care product, comprising any of the skin care active ingredients described above.

[0022] In one embodiment, the skin care product comprises 0.41-23 parts by weight of the skin care active ingredient and 6-16.5 parts by weight of the base material.

[0023] In one embodiment, the skin care product consists of the skin care active, the base and deionized water.

[0024] In one embodiment, the base material is composed of the following components in the following weight ratios: 2-6 parts by weight of glycerin, 1-3 parts by weight of polydimethylsiloxane, 1-4 parts by weight of squalane, 1-1.5 parts by weight of acrylic acid or acrylate or C10-30 alkyl acrylate crosspolymer, and 1-2 parts by weight of polysorbate 60.

[0025] The present application has the beneficial effects of: Different from the prior art, the present application discloses a skin care active ingredient and a skin care product; the skin care active ingredient comprises, by weight, 0.31-13 parts of an antioxidant and 0.1-10 parts of a moisturizer; wherein the antioxidant comprises 0.1-5 parts of VC glucoside, 0.1-2 parts of tocopheryl acetate, 0.01-1 parts of silymarin, and 0.1-5 parts of unicorn extract; and the moisturizer comprises at least one of sodium hyaluronate cross-polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate. By designing the above ingredients and their proportions, the synergistically formed active ingredient can gently and efficiently produce antioxidant effects, effectively protect the skin barrier, maintain skin health, and is highly safe.

[0026] The above description is only an overview of the technical solution of the present application. In order to more clearly understand the technical means of the present application, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are listed below. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0028] FIG1 is a comparison chart of cell proliferation activity test results;

[0029] Figure 2 is a comparison chart of ROS content detection;

[0030] Figure 3 is a comparison chart of ROS content detection data;

[0031] Figure 4 is a comparison of mitochondrial membrane potential;

[0032] Figure 5 is a comparison diagram of mitochondrial membrane potential test data;

[0033] Figure 6 is a comparison of cell scratches;

[0034] Figure 7 is a comparison chart of cell scratch test data;

[0035] FIG8 is a comparison chart of HAcaT cell proliferation activity;

[0036] FIG9 is a graph comparing the proliferation activity of HSF cells. DETAILED DESCRIPTION

[0037] In order to make the purpose, technical solution and effect of this application clearer and more specific, the following embodiments of the technical solution of this application will be described in detail with reference to the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of this application and are therefore only examples and are not intended to limit the scope of protection of this application.

[0038] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application and the above-mentioned figure descriptions are intended to cover non-exclusive inclusions.

[0039] In the description of the embodiments of the present application, the technical terms "first" and "second" are used only to distinguish different objects and should not be understood as indicating or implying relative importance or implicitly indicating the quantity, specific order, or primary and secondary relationship of the indicated technical features. In the description of the embodiments of the present application, the term "multiple" refers to more than two (including two), similarly, "multiple groups" refers to more than two (including two), and "multiple pieces" refers to more than two (including two), unless otherwise clearly and specifically defined.

[0040] References herein to "embodiments" mean that a particular feature, structure, or characteristic described in connection with the embodiments may be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor does it constitute an independent or alternative embodiment that is mutually exclusive of other embodiments. It is understood, both explicitly and implicitly, by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0041] In the description of the embodiments of this application, the term "and / or" is simply a description of the association relationship between associated objects, indicating that three relationships can exist. For example, A and / or B can represent the following three situations: A exists alone, A and B exist simultaneously, and B exists alone. In addition, the character " / " in this document generally indicates that the associated objects are in an "or" relationship.

[0042] Amounts, ratios, and other numerical values ​​are presented herein in a range format. It should be understood that such range format is used for convenience and brevity and should be interpreted flexibly to include not only the values ​​explicitly specified as range limits, but also all individual values ​​or sub-ranges encompassed within the range, as if each value and sub-range were explicitly specified.

[0043] If not otherwise specified, all steps of the present application may be performed sequentially, randomly, or in parallel, preferably sequentially. For example, the method includes steps (a) and (b), indicating that the method may include steps (a) and (b) performed sequentially, or may include steps (b) and (a) performed sequentially, or may include steps (a) and (b) performed simultaneously in parallel. For example, the method may further include step (c), indicating that step (c) may be added to the method in any order, for example, the method may include steps (a), (b), and (c), or may include steps (a), (c), and (b), or may include steps (c), (a), and (b), etc.

[0044] Human skin is the body's largest organ and its first line of defense against external aggression. Oxidative stress, a series of biological reactions triggered by excessive reactive oxygen species in response to internal or external environmental factors, is often accompanied by an increase in free radicals. Oxidative stress not only alters the structure and function of proteins, lipids, and DNA at the molecular level, but also triggers cell apoptosis and extracellular matrix degradation, ultimately leading to inflammation and aging.

[0045] Excessive ultraviolet (UV) radiation can affect the appearance of the skin and accelerate skin aging, resulting in symptoms such as roughness, dryness, irregular pigmentation, decreased elasticity, and wrinkles. This phenomenon is often referred to as photoaging. Oxidative stress is the primary mechanism causing skin photoaging. Under normal circumstances, organisms possess a comprehensive antioxidant system that maintains a metabolic balance of free radicals. When ultraviolet (UV) radiation irradiates the skin, a large number of free radicals are generated, inhibiting the activity of antioxidant enzymes such as superoxide dismutase and catalase, thereby reducing the body's ability to scavenge free radicals. This leads to further accumulation of free radicals, causing severe oxidative damage to intracellular biomolecules such as DNA, lipids, and proteins, and accelerating aging.

[0046] Based on this, the present application provides a skin care active ingredient and skin care product that can gently and efficiently produce antioxidant effects, effectively protect the skin barrier, maintain skin health, and have high safety.

[0047] The present invention provides a skin care active ingredient. The skin care active ingredient comprises, by weight, 0.31-13 parts of an antioxidant and 0.1-10 parts of a moisturizer. The antioxidant comprises 0.1-5 parts of VC glucoside, 0.1-2 parts of tocopheryl acetate, 0.01-1 parts of silymarin, and 0.1-5 parts of unicorn extract; and the moisturizer comprises at least one of sodium hyaluronate crosspolymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate.

[0048] Among them, VC glucoside can effectively resist oxidation, remove yellowness and brighten skin. Tocopheryl acetate has strong reducing properties, has antioxidant effects, and scavenges free radicals. Silymarin has extremely strong antioxidant and free radical scavenging activity, promoting the production of collagen and hyaluronic acid. Kirin extract can improve microcirculation and has anti-inflammatory and antioxidant effects. Hydrolyzed sodium hyaluronate, sodium hyaluronate, sodium hyaluronate crosspolymer, and acetylated sodium hyaluronate all act as skin hydrating factors, which can strengthen the skin barrier, lock in moisture, achieve moisturizing effects, and improve skin condition. Hydrolyzed sodium hyaluronate, sodium hyaluronate, sodium hyaluronate crosspolymer, and acetylated sodium hyaluronate combine to form multiple hyaluronic acids of different molecular weights, covering the skin surface to the deep layers of the skin, effectively moisturizing and repairing the skin barrier.

[0049] By designing skin care active ingredients including a variety of antioxidants with antioxidant effects, the skin is protected from oxidation through multiple pathways, and they work together to achieve efficient and gentle antioxidant effects, improve skin condition, and maintain skin health.

[0050] In addition, the weight proportions of the various components of the skin care actives are designed as above, so that the skin care actives can act on the skin to have antioxidant and moisturizing effects, and the effects are long-lasting, while not burdening the skin. It is highly safe, gently and efficiently improves skin condition, effectively protects the skin barrier, and maintains skin health.

[0051] It's important to note that healthy skin refers to skin that is smooth, soft, hydrated, even, firm, elastic, and flawless, based on the normal functioning of its physiological functions. Health is the foundation of beauty, and only healthy skin can achieve beauty. By empowering the skin with "balance, resilience, and self-healing abilities," we can gently and effectively maintain healthy skin.

[0052] Balance: The skin's surface and underlying layers, the stratum corneum and basal layer, each tightly bound together, enable the skin to maintain moisture and prevent water loss. The skin's ability to absorb and retain water, as well as the skin cells' ability to absorb and utilize oxygen, influence the skin's water balance and moisturizing capacity, as well as its metabolism and health, including its ability to heal itself.

[0053] Self-healing ability: The skin's ability to repair itself from damage, trauma, cell aging, etc., as well as the skin cells' ability to absorb and metabolize nutrients, affect the skin's metabolism and repair ability.

[0054] Resistance: The skin's ability to resist external environmental factors, including sunlight, pollutants, ultraviolet rays, bacteria, etc., as well as the skin cells' ability to identify and resist external pathogens and viruses, affect the health and appearance of the skin.

[0055] In this application, VC glucoside corresponds to "resistance," tocopheryl acetate corresponds to "balance," silymarin corresponds to "resistance," hydrolyzed sodium hyaluronate, sodium hyaluronate, sodium hyaluronate crosspolymer, and acetylated sodium hyaluronate correspond to "balance," and unicorn extract corresponds to "self-healing power." This application designs skin care active ingredients to include VC glucoside, tocopheryl acetate, silymarin, unicorn extract, and at least one of "hydrolyzed sodium hyaluronate, sodium hyaluronate, sodium hyaluronate crosspolymer, and acetylated sodium hyaluronate," so that the skin care active ingredients simultaneously include "self-healing power," "resistance," and "balance," fundamentally improving the skin and gently and efficiently maintaining healthy skin.

[0056] From another perspective, based on the framework of "balance, resistance, and self-healing power", the use of a combination of active ingredients that are "homologous to the human body and originate from plants" can gently and efficiently achieve antioxidant and moisturizing effects, and promote the skin to a healthy state. The sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, hydrolyzed sodium hyaluronate, VC glucoside, and tocopherol acetate in this application are ingredients that are homologous to the human body, and unicorn extract and silymarin are ingredients that are of plant origin. This application designs skin care active ingredients including VC glucoside, tocopherol acetate, silymarin, unicorn extract, and at least one of "hydrolyzed sodium hyaluronate, sodium hyaluronate, sodium hyaluronate cross-linked polymer, and acetylated sodium hyaluronate", so that the skin care active ingredients have both human homologous ingredients and plant origin ingredients, and achieve antioxidant and moisturizing effects efficiently and gently.

[0057] In one embodiment, the skin care active comprises, by weight, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate. The 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate means that the total weight of these four substances in the skin care active is 0.1-10 parts by weight. In the 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate, the weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2). It is understood that when the moisturizing agent of the skin care active comprises sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the skin care active comprises 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate. Exemplarily, in the 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate, the weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate can be 0.3:0.5:1:0.15, 0.2:0.7:0.8:0.1, 0.5:0.8:1.2:0.2, 0.4:1:1:0.1, etc. The 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate in the following content are the same as above and will not be described in detail.

[0058] Optionally, the skin care active ingredients include, by weight, a sodium hyaluronate cross-linked polymer having a molecular weight greater than 3000KDa (kilodaltons), a sodium hyaluronate having a molecular weight of 1000-1800KDa, an acetylated sodium hyaluronate having a molecular weight of 1000KDa, and a hydrolyzed sodium hyaluronate having a molecular weight of less than 10KDa. By designing the molecular weights of the sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the skin is covered from the surface layer to the deep layer of the skin, the skin barrier function is strengthened, moisture is locked, and a good moisturizing effect is achieved. It should be noted that when the skin care active ingredient moisturizer is composed of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the molecular weight selection of the above-mentioned sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is also applicable to the following content.

[0059] In one embodiment, the skin care active comprises 0.1-10 parts by weight of hydrolyzed sodium hyaluronate. It is understood that when the moisturizing agent of the skin care active comprises hydrolyzed sodium hyaluronate, the skin care active comprises 0.1-10 parts by weight of hydrolyzed sodium hyaluronate.

[0060] In one embodiment, the skin care active comprises, by weight, 0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate. It will be appreciated that when the moisturizing agent for the skin care active comprises sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the skin care active comprises 0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate. The term "0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate" refers to a total weight ratio of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate in the skin care active of 0.1-10 parts by weight. The weight ratio of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1. The 0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate described below are the same and are not further described. For example, in the 0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the weight ratio of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate can be 4.5:5.5:1, 5:6:1, 5.5:6.5:1, etc.

[0061] In one embodiment, the skin care active comprises, by weight, 0.1-10 parts by weight of a sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate. It will be appreciated that when the moisturizing agent for the skin care active comprises a sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the skin care active comprises 0.1-10 parts by weight of a sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate. The term "0.1-10 parts by weight of a sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate" refers to a total weight ratio of the three substances, sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, in the skin care active. The weight ratio of the sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1. The 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate described below are the same and are not further described. For example, in the 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the ratio of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate can be 4.5:5.5:1, 5:6:1, 5.5:6.5:1, etc.

[0062] In one embodiment, the skin care active comprises, by weight, 0.1-10 parts by weight of a sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate. It will be appreciated that when the moisturizing agent for the skin care active comprises sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate, the skin care active comprises 0.1-10 parts by weight of a sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate. The term "0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate" refers to a total weight ratio of 0.1-10 parts by weight of these three substances in the skin care active. The weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1. The 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate described below are the same as above and are not further described. For example, in the 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate, the weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate can be 4.5:5.5:1, 5:6:1, 5.5:6.5:1, etc.

[0063] In one embodiment, the skin care active comprises, by weight, 0.1-10 parts by weight of sodium hyaluronate. It is understood that when the skin care active moisturizer comprises sodium hyaluronate, the skin care active comprises 0.1-10 parts by weight of sodium hyaluronate.

[0064] In one embodiment, when the antioxidant consists of VC glucoside, tocopheryl acetate, silymarin, and unicorn extract, the skin care active comprises 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, and 0.1-5 parts by weight of unicorn extract.

[0065] In a specific embodiment, the skin care active is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, and the weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2).

[0066] In one specific embodiment, the skin care active is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, and 0.1-10 parts by weight of hydrolyzed sodium hyaluronate.

[0067] In a specific embodiment, the skin care active is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate and hydrolyzed sodium hyaluronate, and the weight ratio of sodium hyaluronate, acetylated sodium hyaluronate and hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1.

[0068] In a specific embodiment, the skin care active is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate and hydrolyzed sodium hyaluronate, and the weight ratio of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate and hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1.

[0069] In a specific embodiment, the skin care active is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate and hydrolyzed sodium hyaluronate, and the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate and the hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1.

[0070] In one embodiment, the skin care active ingredient is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, and 0.1-10 parts by weight of sodium hyaluronate.

[0071] It should be noted that when the skin care active includes VC glucoside, the weight parts of VC glucoside in the skin care active can be 0.1 parts by weight, 0.5 parts by weight, 1 part by weight, 1.5 parts by weight, 2 parts by weight, 2.5 parts by weight, 3 parts by weight, 3.5 parts by weight, 4 parts by weight, 4.5 parts by weight, 5 parts by weight, etc., or a range consisting of any two of the above values, for example, 0.1-2 parts by weight, 1-4 parts by weight, etc.

[0072] When the skin care active includes tocopheryl acetate, the weight amount of tocopheryl acetate in the skin care active can be 0.1 parts by weight, 0.5 parts by weight, 1 part by weight, 1.5 parts by weight, 2 parts by weight, etc., or a range consisting of any two of the above values, such as 0.1-1.5 parts by weight, 0.5-2 parts by weight, etc.

[0073] When the skin care active includes silymarin, the weight amount of silymarin in the skin care active can be 0.01 parts by weight, 0.05 parts by weight, 0.1 parts by weight, 0.15 parts by weight, 0.20 parts by weight, 0.25 parts by weight, 0.3 parts by weight, 0.35 parts by weight, 0.4 parts by weight, 0.45 parts by weight, 0.5 parts by weight, 0.55 parts by weight, 0.6 parts by weight, 0.65 parts by weight, 0.7 parts by weight, 0.75 parts by weight, 0.8 parts by weight, 0.85 parts by weight, 0.9 parts by weight, 0.95 parts by weight, 1 part by weight, etc., or a range formed by any two of the above values, for example, 0.01-0.3 parts by weight, 0.1-0.7 parts by weight, etc.

[0074] When the skin care active includes the kelp extract, the weight portion of the kelp extract in the skin care active can be 0.1 parts by weight, 0.5 parts by weight, 1 part by weight, 1.5 parts by weight, 2 parts by weight, 2.5 parts by weight, 3 parts by weight, 3.5 parts by weight, 4 parts by weight, 4.5 parts by weight, 5 parts by weight, etc., or a range consisting of any two of the above values, for example, 0.5-2 parts by weight, 2-4 parts by weight, etc.

[0075] When the moisturizing agent of the skin care active comprises sodium hyaluronate cross-polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate, the total weight of the sodium hyaluronate cross-polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate in the skin care active can be 0.1 parts by weight, 0.5 parts by weight, 1 part by weight, 1.5 parts by weight, 2 parts by weight, 2.5 parts by weight, 3 parts by weight, 3.5 parts by weight, 4 parts by weight, 4.5 parts by weight, 5 parts by weight, 5.5 parts by weight, 6 parts by weight, 6.5 parts by weight, 7 parts by weight, 7.5 parts by weight, 8 parts by weight, 8.5 parts by weight, 9 parts by weight, 10 parts by weight, etc., or a range consisting of any two of the above values, for example, 0.1-5 parts by weight, 3-8 parts by weight, etc.

[0076] When the moisturizing agent of the skin care active comprises sodium hyaluronate, acetylated sodium hyaluronate, or hydrolyzed sodium hyaluronate, the total weight of the sodium hyaluronate, acetylated sodium hyaluronate, or hydrolyzed sodium hyaluronate in the skin care active can be 0.1 parts by weight, 0.5 parts by weight, 1 part by weight, 1.5 parts by weight, 2 parts by weight, 2.5 parts by weight, 3 parts by weight, 3.5 parts by weight, 4 parts by weight, 4.5 parts by weight, 5 parts by weight, 5.5 parts by weight, 6 parts by weight, 6.5 parts by weight, 7 parts by weight, 7.5 parts by weight, 8 parts by weight, 8.5 parts by weight, 9 parts by weight, 10 parts by weight, etc., or a range consisting of any two of the above values, for example, 0.1-5 parts by weight, 3-8 parts by weight, etc.

[0077] When the moisturizing agent of the skin care active comprises sodium hyaluronate cross-polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the total weight of the sodium hyaluronate cross-polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate in the skin care active can be 0.1 parts by weight, 0.5 parts by weight, 1 part by weight, 1.5 parts by weight, 2 parts by weight, 2.5 parts by weight, 3 parts by weight, 3.5 parts by weight, 4 parts by weight, 4.5 parts by weight, 5 parts by weight, 5.5 parts by weight, 6 parts by weight, 6.5 parts by weight, 7 parts by weight, 7.5 parts by weight, 8 parts by weight, 8.5 parts by weight, 9 parts by weight, 10 parts by weight, etc., or a range consisting of any two of the above values, for example, 0.1-5 parts by weight, 3-8 parts by weight, etc.

[0078] When the moisturizing agent of the skin care active comprises sodium hyaluronate cross-polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate, the total weight of the sodium hyaluronate cross-polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate in the skin care active can be 0.1 parts by weight, 0.5 parts by weight, 1 part by weight, 1.5 parts by weight, 2 parts by weight, 2.5 parts by weight, 3 parts by weight, 3.5 parts by weight, 4 parts by weight, 4.5 parts by weight, 5 parts by weight, 5.5 parts by weight, 6 parts by weight, 6.5 parts by weight, 7 parts by weight, 7.5 parts by weight, 8 parts by weight, 8.5 parts by weight, 9 parts by weight, 10 parts by weight, etc., or a range consisting of any two of the above values, for example, 0.1-5 parts by weight, 3-8 parts by weight, etc.

[0079] When the skin care active includes sodium hyaluronate, the weight amount of sodium hyaluronate in the skin care active can be 0.1 parts by weight, 0.5 parts by weight, 1 part by weight, 1.5 parts by weight, 2 parts by weight, 2.5 parts by weight, 3 parts by weight, 3.5 parts by weight, 4 parts by weight, 4.5 parts by weight, 5 parts by weight, 5.5 parts by weight, 6 parts by weight, 6.5 parts by weight, 7 parts by weight, 7.5 parts by weight, 8 parts by weight, 8.5 parts by weight, 9 parts by weight, 10 parts by weight, etc., or a range consisting of any two of the above values, for example, 0.1-4 parts by weight, 2-8 parts by weight, etc.

[0080] When the skin care active includes hydrolyzed sodium hyaluronate, the weight amount of hydrolyzed sodium hyaluronate in the skin care active can be 0.1 parts by weight, 0.5 parts by weight, 1 part by weight, 1.5 parts by weight, 2 parts by weight, 2.5 parts by weight, 3 parts by weight, 3.5 parts by weight, 4 parts by weight, 4.5 parts by weight, 5 parts by weight, 5.5 parts by weight, 6 parts by weight, 6.5 parts by weight, 7 parts by weight, 7.5 parts by weight, 8 parts by weight, 8.5 parts by weight, 9 parts by weight, 10 parts by weight, etc., or a range consisting of any two of the above values, for example, 0.1-2 parts by weight, 1-7 parts by weight, etc.

[0081] The embodiments of the present application also provide a skin care product, which includes the skin care active ingredients introduced in the above embodiments and has at least the same advantages as the above skin care active ingredients.

[0082] In one embodiment, the skin care product comprises, by weight, 0.41-23 parts by weight of a skin care active and 6-16.5 parts by weight of a base. The base typically includes a moisturizing ingredient. It should be noted that the composition and proportions of the skin care active are as described above and will not be further elaborated.

[0083] The combination of skin care actives and base materials forms a skin care product that can gently and effectively produce antioxidant effects, effectively protecting the skin barrier and maintaining skin health.

[0084] In one embodiment, the skin care product comprises a skin care active ingredient, a base, and deionized water. Optionally, the amount of deionized water to be added is calculated from the total weight of the skin care product being 100 parts by weight minus the sum of the weight of the skin care active ingredient and the weight of the base.

[0085] In one embodiment, the dosage form of the skin care product includes any one of a water-based solution, an emulsion, a cream, an essence lotion, a facial mask, and a spray. The skin care active ingredient can achieve antioxidant efficacy in any of these dosage forms, and the dosage form is selected based on specific needs; the base ingredients are designed based on the desired dosage form.

[0086] In one embodiment, the base comprises the following components in the following weight ratios: 2-6 parts by weight of glycerin, 1-3 parts by weight of polydimethylsiloxane, 1-4 parts by weight of squalane, 1-1.5 parts by weight of acrylic acid, acrylic acid esters, or C10-30 alkyl acrylate crosspolymer, and 1-2 parts by weight of polysorbate 60. This base is suitable for use in serum emulsion formulations, where polysorbate 60 provides an emulsifying effect and acrylic acid esters provide a thickening effect.

[0087] In one embodiment, the skin care product comprises the following components in the following weight ratios: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate crosspolymer, sodium hyaluronate, acetylated sodium hyaluronate, or hydrolyzed sodium hyaluronate, 2-6 parts by weight of glycerin, 1-3 parts by weight of polydimethylsiloxane, 1-4 parts by weight of squalane, 1-1.5 parts by weight of acrylic acid, acrylates, or C10-30 alkyl acrylate crosspolymer, 1-2 parts by weight of polysorbate 60, and the remaining parts by weight of deionized water. The total weight of the skin care product is 100 parts. The weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate and hydrolyzed sodium hyaluronate is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2).

[0088] The skin care actives provided herein have antioxidant properties, and relevant experimental testing and research have been conducted at the cellular and biochemical reagent levels. Taking a skin care product composed of the following components in the following weight ratio: 1 part by weight of VC glucoside, 1 part by weight of tocopheryl acetate, 1 part by weight of silymarin, 1 part by weight of unicorn extract, 0.4 part by weight of sodium hyaluronate crosspolymer, 1 part by weight of sodium hyaluronate, 1 part by weight of acetylated sodium hyaluronate, 0.1 part by weight of hydrolyzed sodium hyaluronate, 3 parts by weight of glycerin, 1 part by weight of polydimethylsiloxane, 1 part by weight of squalane, 1 part by weight of C10-30 alkyl acrylate crosspolymer, 1 part by weight of polysorbate 60, and 86.5 parts by weight of deionized water as an example, experimental testing at the cellular and biochemical reagent levels was conducted.

[0089] 1. Cell proliferation activity assay.

[0090] Experimental steps:

[0091] (1) HSF cells were seeded into 96-well plates, and 100 μL of culture medium containing 8*103 HSF cells was added to each well. The plates were incubated at 37°C for 24 h until the cells adhered to the plate and entered the logarithmic growth phase.

[0092] (2) After 24 hours, the culture medium in step (1) was discarded, and 100 μL of the test solution of different concentrations was added to each well. Three parallel wells were set for each concentration, and the cells were incubated for 24 hours.

[0093] (3) Aspirate the test solution added in step (2), add 90 μL of fresh culture medium and 10 μL of CCK-8 solution (Cell Counting Kit-8 reagent), and incubate for 1 to 4 hours.

[0094] (4) Measure the absorbance at 450 nm using an enzyme-labeled instrument.

[0095] Calculation method: Cell viability*(%)=[A(sample added)-A(blank) / [A(0 sample added)-A(blank)]×100;

[0096] Wherein, A(sample added) represents the absorbance of the wells containing cells, CCK-8 solution, and test solution;

[0097] A (blank) indicates the absorbance of wells with culture medium and CCK-8 solution but no cells;

[0098] A(0 loading) represents the absorbance of the wells containing cells and CCK-8 solution but no test solution;

[0099] Cell viability* indicates cell proliferation activity or cytotoxic activity.

[0100] It should be noted that when adding the culture medium containing CCK-8 to the cells in step (3), several blank wells without cells are added simultaneously to correct the values, which are referred to as A (blank). In step (2), several wells do not contain the test solution, but only the cell culture medium, which are referred to as A (0 addition).

[0101] Experimental results:

[0102] The experimental results are shown in Figure 1, which is a comparison chart of the cell proliferation activity test results. According to Figure 1, the cell proliferation activity test was conducted on the skin care active ingredients at concentrations of 5%, 1%, 0.3%, 0.1%, 0.05%, and 0.01%, respectively. The concentration here refers to the concentration of the skin care product diluted with cell culture medium, i.e., the "different concentrations of test solution" in step (2). Excellent proliferation activity was expressed at concentrations of 1%, 0.1%, 0.05%, and 0.01%, with increases of 5.3%, 2.1%, 4.8%, and 3.4%, respectively.

[0103] 2. ABTS free radical scavenging experiment.

[0104] Experimental steps:

[0105] (1) Reagent preparation:

[0106] ① 7 mmol / L ABTS (18.0116 mg ABTS powder diluted in 5 ml distilled water).

[0107] ②2.4mmol / L potassium sulfate (32.4384mg potassium sulfate powder diluted in 50ml distilled water)

[0108] ③ Stir the ABTS from step ① and the potassium sulfate from step ② in a 1:1 ratio (10 ml in total).

[0109] ④Step ③ react at room temperature in the dark for 12 to 24 hours.

[0110] ⑤ Dilute 2 ml of ④ in 40 ml of distilled water to an OD value of ± 0.7 (final solution).

[0111] (2) Sample addition: Add 20 μL of sample to each well, then add 180 μL of ABTS detection solution, and react at room temperature in the dark for 7 minutes.

[0112] (3) Detection: The absorbance at 718 nm was measured using an enzyme-labeled instrument, and the inhibition rate was calculated.

[0113] Calculation method: inhibition rate % = (1-A1 / A0) × 100%;

[0114] Where A0 represents the absorbance value of the blank group at 517 nm; A1 represents the absorbance value of the sample group at 517 nm. It should be noted that the sample is the skin care product of this application. The blank group refers to the wells without sample added.

[0115] Experimental results:

[0116] The experimental results are shown in Table 1. The skin care active substance of the present application was tested for ABTS free radical scavenging rate at concentrations of 1%, 5%, and 10%. The experimental results showed that the skin care active substance can significantly inhibit ABTS free radicals and has significant antioxidant effect.

[0117] Table 1 ABTS free radical scavenging experimental results

[0118] 3. DPPH free radical scavenging experiment.

[0119] Experimental steps:

[0120] (1) Reagent preparation: Prepare a 150 μM DPPH (i.e., 150 μmol / L DPPH) solution in 80% ethanol.

[0121] (2) Sample addition: Add 20 μL of sample to each well, then add 180 μL of DPPH solution, and react at room temperature in the dark for 30 minutes.

[0122] (3) Detection: The absorbance at 517 nm was measured using an enzyme-labeled instrument, and the inhibition rate was calculated.

[0123] Calculation method: inhibition rate % = (1-A1 / A0) × 100%;

[0124] Wherein, A0 represents the absorbance value of the blank group at 517 nm; A1 represents the absorbance value of the sample group at 517 nm.

[0125] It should be noted that the sample is the skin care product of this application. The blank group refers to the wells without sample added.

[0126] In the DPPH free radical scavenging experiment, the skin care product provided in the embodiment of the present application, VC glucoside, tocopheryl acetate, silymarin, quadruple hyaluronic acid (quadruple hyaluronic acid includes sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate), and kelp extract were used as samples in step (2) and the experiment was carried out in sequence. The concentration of VC glucoside was the same as the concentration of VC glucoside in the skin care active of the skin care product; the concentration of tocopheryl acetate was the same as the concentration of tocopheryl acetate in the skin care active of the skin care product; the concentration of silymarin was the same as the concentration of silymarin in the skin care active of the skin care product; the concentration of quadruple hyaluronic acid was the same as the concentration of quadruple hyaluronic acid in the skin care active and had the same composition as the quadruple hyaluronic acid in the skin care active; the concentration of kelp extract was the same as the concentration of kelp extract in the skin care active of the skin care product.

[0127] Experimental results:

[0128] The experimental results are shown in Table 2 below. For the skin care active provided in this application, a DPPH free radical scavenging rate test was conducted at a concentration of 10%, and compared with each active substance monomer. The skin care active provided in this application can significantly inhibit DPPH free radicals, has significant antioxidant efficacy, and is superior to the efficacy of each active substance monomer at the same concentration, reflecting the synergistic efficiency of the composition. It should be noted that the concentration of VC glucoside in the skin care active at a concentration of 10% is equivalent to 0.5%, the concentration of tocopheryl acetate in the skin care active at a concentration of 10% is equivalent to 0.01%, the concentration of silymarin in the skin care active at a concentration of 10% is equivalent to 0.1%, the concentration of quadruple hyaluronic acid in the skin care active at a concentration of 10% is equivalent to 0.1%, and the concentration of unicorn extract in the skin care active at a concentration of 10% is equivalent to 0.1%.

[0129] Table 2 DPPH test results of skin care actives and active monomers of skin care actives

[0130] 4. Tyrosinase activity test.

[0131] Experimental steps:

[0132] Refer to Table 3 for reagent addition amounts. Add L-tyrosine solution, sample solution / solvent, and PBS buffer to each well in sequence, mix thoroughly, and incubate at 37°C for 10 minutes. Then, add 20 μL of tyrosinase solution to each well. Mix and react at 37°C for 5 minutes ± 5 seconds. Immediately place the well in a microplate reader and measure at a wavelength of 475 nm. The sample solution in Table 3 refers to the skin care actives provided herein.

[0133] Table 3 Tyrosinase activity test reagent addition amount

[0134] Experimental results:

[0135] The experimental results are shown in Table 4. For the skin care active provided in the examples of the present application, a tyrosinase activity inhibition rate test was carried out at a concentration of 10%. The experimental results show that the skin care active can significantly inhibit the activity of tyrosinase and has a significant whitening effect.

[0136] Table 4 Tyrosinase activity inhibition test results

[0137] 5. ROS content detection experiment.

[0138] Experimental steps:

[0139] (1) Load the probe.

[0140] (a) Cell preparation: Cells were plated one day before the assay to ensure that the cell confluence reached 50-70% at the time of assay.

[0141] (b) Sample addition: After 24 hours, the culture medium was discarded and 100 μL of a 0.05% concentration of the test solution was added to each well. Three parallel wells were set up and incubated for 24 hours. The culture medium was a 9:1 mixture of DMEM and fetal bovine serum.

[0142] (c) Drug induction: Remove the cell culture medium, dilute H2O2 to a concentration of 2 mmol / L with serum-free medium, and incubate in a cell culture incubator at 37°C in the dark for 4 h.

[0143] (d) Probe preparation: Before probe loading, DCFH-DA was diluted 1:1000 in serum-free culture medium to a final concentration of 10 μM.

[0144] (e) Probe loading: Remove the induction drug by aspiration and add an appropriate volume of diluted DCFH-DA working solution, sufficient to cover the cells. For example, for a 6-well plate, this should be at least 1000 μL, and for a 96-well plate, this should be at least 100 μL. Incubate in a 37°C cell culture incubator in the dark for 30 min.

[0145] (f) Cell washing: Wash the cells 1 to 2 times with serum-free culture medium to fully remove DCFH-DA that has not entered the cells.

[0146] (2) Detection.

[0147] Experimental results:

[0148] The ROS content detection experiment is shown in Figures 2 and 3. Figure 2 is a comparison chart of ROS content detection, and Figure 3 is a comparison chart of ROS content detection data. According to Figures 2 and 3, the skin care composition provided in this application can significantly scavenge ROS free radicals and has a significant antioxidant effect. It should be noted that the blank control group did not add samples and did not undergo H2O2 induction; the model group did not add samples and underwent H2O2 induction; skin care products refer to those that have been induced with H2O2 while adding skin care active ingredients.

[0149] 6. Mitochondrial membrane potential detection experiment.

[0150] Experimental steps:

[0151] (1) Cell preparation: Cells were plated one day before the test to ensure that the cell confluence reached 50-70% at the time of the test.

[0152] (2) Sample addition: After 24 hours, the culture medium was discarded and 100 μL of 0.05% concentration of the test solution was added to each well. Three parallel wells were set up and incubated for 24 hours. The culture medium was prepared by mixing DMEM with fetal bovine serum in a ratio of 9:1.

[0153] (3) Aspirate the culture medium and wash the cells once with PBS or other appropriate solution if necessary according to the specific experiment.

[0154] (4) Drug induction: Remove the cell culture medium, dilute H2O2 to a concentration of 2 mmol / L with serum-free culture medium, and incubate in a cell culture incubator at 37°C in the dark for 4 hours.

[0155] (5) Aspirate the culture medium, wash the cells once with PBS, add 1 mL of JC-1 staining solution, and mix thoroughly. Incubate in a cell culture incubator at 37°C for 20 minutes. The JC-1 stock solution is 500X (that is, 500 times, the concentration of the staining solution is 1 times, that is, 1X, and the JC-1 staining solution is the 1X concentration after dilution).

[0156] (6) During the incubation period, prepare an appropriate amount of JC-1 staining buffer (1×) by adding 4 mL of distilled water to every 1 mL of JC-1 staining buffer (5×) and place it in an ice bath.

[0157] (7) After incubation at 37°C, remove the supernatant and wash twice with JC-1 staining buffer (1×).

[0158] (8) Add 2 mL of cell culture medium containing serum and phenol red.

[0159] (9) Observe under a fluorescence microscope or laser confocal microscope.

[0160] Experimental results:

[0161] The experimental results are shown in Figures 4 and 5. Figure 4 is a comparison chart of mitochondrial membrane potential, and Figure 5 is a comparison chart of mitochondrial membrane potential test data. As shown in Figures 4 and 5, the skin care products provided in the examples of the present application can significantly reduce damage to mitochondria and have a significant mitochondrial protective effect. It should be noted that the blank control group did not add samples and did not undergo H2O2 induction; the H2O2 model group did not add samples and underwent H2O2 induction; skin care products refer to those that have skin care active ingredients added and H2O2 induction.

[0162] 7. Cell scratch test.

[0163] Experimental steps:

[0164] (1) HaCaT cells were seeded into 12-well plates and incubated at 37°C for 24 h. The cells adhered to the plate and entered the logarithmic growth phase.

[0165] (2) After 24 hours, discard the culture medium and add 100 μL of 0.05% concentration test solution to each well. Set up three parallel wells and incubate for 24 hours.

[0166] (3) Use a 100 μl pipette tip to make three parallel slits in each well, wash with PBS to remove the cells, add serum-free culture medium, take a photo under a microscope at 0 h, and continue to culture in the incubator for 24 h.

[0167] (4) After 24 hours, take photos under a microscope.

[0168] (5) Calculate the cell migration rate after 24 hours.

[0169] Experimental results:

[0170] The experimental results are shown in Figures 6 and 7. Figure 6 is a cell scratch comparison diagram, and Figure 7 is a cell scratch test data comparison diagram. As shown in Figures 6 and 7, the skin care products provided in the embodiments of the present application can significantly promote cell migration rate and have a significant repair effect; compared with the cell migration rate of the blank control group of 36.7%, the cell migration rate of the skin care product composition provided in the embodiments of the present application is 56.8%, an increase of 20.1%, and the increase rate is (56.8%-36.7%) / 36.7%=54.8%. Among them, the blank control group is a number of wells in step (2) in which no test solution is added.

[0171] 8. Cell proliferation activity experiment after UVB irradiation.

[0172] Experimental steps:

[0173] (1) Cells were seeded into 96-well plates, and 100 μL of culture medium containing 8 × 103 cells was added to each well. The plates were incubated at 37°C for 24 h until the cells adhered to the plate and entered the logarithmic growth phase.

[0174] (2) After 24 hours, discard the culture medium and add 100 μL of 0.05% concentration test solution to each well. Set up three parallel wells and incubate for 24 hours.

[0175] (3) The culture medium containing the test solution was aspirated, 20 μL PBS was added, and UVB irradiation was performed for 30 mJ. The PBS was aspirated and the culture medium was added to recover for 24 h.

[0176] (4) Aspirate the culture medium, add 90 μL of fresh culture medium and 10 μL of CCK-8 solution, and incubate for 1 to 4 hours.

[0177] (5) Measure the absorbance at 450 nm using an enzyme-labeled instrument.

[0178] Calculation method: Cell viability*(%)=[A(sample added)-A(blank) / [A(0 sample added)-A(blank)]×100;

[0179] Wherein, A(sample added) represents the absorbance of the wells containing cells, CCK-8 solution, and test solution;

[0180] A (blank) indicates the absorbance of wells with culture medium and CCK-8 solution but no cells;

[0181] A(0 loading) represents the absorbance of the wells with cells and CCK-8 solution but no test solution;

[0182] Cell viability* indicates cell proliferation activity or cytotoxic activity.

[0183] It should be noted that when adding the culture medium containing CCK-8 to the cells in step (3), several blank wells without cells are added simultaneously to correct the values, which are referred to as A (blank). In step (2), several wells do not contain the test solution, but only the cell culture medium, which are referred to as A (0 addition).

[0184] Experimental results:

[0185] The experimental results are shown in Figures 8 and 9. Figure 8 is a comparison of HAcaT cell proliferation activity, and Figure 9 is a comparison of HSF cell proliferation activity. According to Figures 8 and 9, the skin care actives provided by this application significantly enhance cell activity under UVB and have a significant anti-photodamage effect. Among them, the blank control is a group without the addition of the test solution (the test solution is the skin care active) and no UVB irradiation; the UVB group is a group without the addition of the test solution and irradiation with UVB; the skin care product group is a group with the addition of the skin care active and irradiation with UVB.

[0186] Through testing at the cellular and biochemical levels, including cell proliferation activity, ABTS free radical scavenging, DPPH free radical scavenging, tyrosinase activity, ROS content, mitochondrial membrane potential, cell scratching, and cell proliferation activity after UVB irradiation, the skin care active ingredients in this application were verified to effectively promote antioxidant efficacy. The DPPH free radical scavenging rate of the skin care active ingredients was higher than that of each individual component, demonstrating the synergistic and efficient nature of the combination.

[0187] Experimental studies were conducted on the components and amounts of the skin care actives provided herein. The differences between the examples are as follows: the components and amounts of the skin care actives are shown in Table 5. The base used in each example is the same, specifically: 4 parts by weight of glycerin, 2 parts by weight of polydimethylsiloxane, 1 part by weight of squalane, 1 part by weight of acrylates, 2 parts by weight of polysorbate 60, and the remaining parts by weight being deionized water, for a total of 100 parts by weight. Where the hyaluronic acid component in each example includes multiple components, the number of parts is the total number of parts of the multiple components. In Examples 1-8, the weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is the same, which is 0.4:1:1:0.1; in Example 10, the weight ratio of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is 5:6:1; in Example 11, the weight ratio of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is 5:6:1; in Example 12, the weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate is 5:6:1.

[0188] Table 5 Test parameters of various embodiments

[0189] As can be seen from Examples 1 to 13 above, the skin care actives provided in the present application have antioxidant properties. It should be noted that the specific testing methods for HSF cell proliferation activity and DPPH clearance in each example in Table 5 can be found in the relevant descriptions in the above examples and will not be repeated here.

[0190] The above description is only an implementation method of the present application and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the description and drawings of this application, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A skin care active ingredient, wherein: The skin care active ingredients include 0.31-13 parts by weight of an antioxidant and 0.1-10 parts by weight of a moisturizer; The antioxidant comprises 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, and 0.1-5 parts by weight of unicorn extract; and the moisturizing agent comprises at least one of sodium hyaluronate cross-polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate.

2. The skin care active according to claim 1, wherein The skin care active ingredients include, by weight, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2); or, The skin care active ingredient comprises, by weight, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate; or The skin care active ingredients include, by weight, 0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1; or, The skin care active ingredients include, by weight, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1; or The skin care active ingredients include, by weight, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1; or The skin care active ingredient comprises, by weight, 0.1-10 parts by weight of sodium hyaluronate.

3. The skin care active according to claim 1, wherein The skin care active ingredient is composed of the following components in the following weight ratios: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopherol acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2).

4. The skin care active according to claim 1, wherein The skin care active ingredient is composed of the following components in the following weight ratios: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopherol acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, and 0.1-10 parts by weight of hydrolyzed sodium hyaluronate.

5. The skin care active according to claim 1, wherein The skin care active ingredient is composed of the following components in the following weight ratios: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopherol acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate and hydrolyzed sodium hyaluronate, the weight ratio of the sodium hyaluronate, the acetylated sodium hyaluronate and the hydrolyzed sodium hyaluronate being (4.5-5.5):(5.5-6.5):

1.

6. The skin care active according to claim 1, wherein The skin care active ingredient is composed of the following components in the following weight ratios: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopherol acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the acetylated sodium hyaluronate and the hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1; or The skin care active ingredient is composed of the following components in the following weight ratios: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopherol acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1; or The skin care active ingredient is composed of the following components in the following weight ratios: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopherol acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, and 0.1-10 parts by weight of sodium hyaluronate.

7. A skin care product, wherein: Comprising the skin care active of any one of claims 1 to 6.

8. The skin care product according to claim 7, wherein: The skin care product comprises 0.41-23 parts by weight of the skin care active ingredient and 6-16.5 parts by weight of a base material.

9. The skin care product according to claim 8, wherein: The skin care product consists of the skin care active substance, the base material and deionized water.

10. The skin care product according to claim 8 or 9, wherein: The base material is composed of the following components in the following weight ratios: 2-6 parts by weight of glycerin, 1-3 parts by weight of polydimethylsiloxane, 1-4 parts by weight of squalane, 1-1.5 parts by weight of acrylic acid or acrylate or C10-30 alkyl acrylate cross-polymer, and 1-2 parts by weight of polysorbate 60.

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