Arc microbial agent for toxicity control, nitrogen fixation and yield increase, and method for producing crop

Abstract

Provided are an ARC microbial agent for toxicity control, nitrogen fixation and yield increase, and a method for producing a crop by using same. The microbial agent is a microbial composition, has a coupled effect of toxicity control and nitrogen fixation, and has the effects of regulating and improving the abundance of rhizobia in the rhizosphere of a leguminous crop and increasing the number of nodules in the leguminous crop. The microbial agent contains DNA sequences as shown in SEQ ID NOs: 1-4.

Description

ARC microbial agent for controlling toxicity and fixing nitrogen and increasing yield and crop production method Technical Field

[0001] The present invention belongs to the field of microorganisms, and in particular relates to an ARC microbial agent for controlling toxicity and fixing nitrogen and increasing yield, and a crop production method. Background Art

[0002] Oilseeds are important sources of fat and plant protein, two of the three major nutrients for the human body. They play a crucial role in ensuring national grain and oil security, maintaining people's nutritional health, and promoting rural revitalization. Soybeans and peanuts are important grain crops, oil crops, and feed crops in my country, and are fundamental, critical, and strategic industries that are crucial to the national economy and people's livelihood.

[0003] The soybean and peanut industries face two common challenges: First, peanuts and soybeans are susceptible to contamination with highly toxic and carcinogenic aflatoxins, which not only reduce quality and production, but also pose a serious threat to people's health and lives. Aflatoxin B1, for example, is 10 times more toxic than potassium cyanide and is classified as a Class I carcinogen by the World Health Organization's International Agency for Research on Cancer. It causes 28.2% of liver cancer cases worldwide. In recent years, aflatoxin contamination in peanuts has generally increased and has become a major risk factor for the peanut and other industries. Existing methods for controlling aflatoxin in peanuts and soybeans primarily rely on temperature and humidity control during storage, transportation, and processing, resulting in high energy consumption and difficult control. Aflatoxin contamination prevention and control remains a global challenge.

[0004] Secondly, legume crops like soybeans and peanuts, while nodulating and fixing nitrogen symbiotically with soil rhizobia, naturally have few nodules, a short nitrogen fixation period (it's generally believed that no nodules and nitrogen fixation occur in the first month after sowing, and no new nodules form during the pod-filling or fruit-full stages, while existing nodules begin to wither), and low efficiency. Research on biological nitrogen fixation with rhizobia has been ongoing for over 100 years, establishing the classically recognized AON theory—that plants self-regulate nodule number and growth while maintaining total energy conservation. Excessive nodulation inevitably comes at the expense of plant growth. Current approaches primarily rely on selecting and applying optimized rhizobia adapted to specific production environments. However, these applications are limited to specific regions and have long been constrained by the AON theory. Improvements in nodulation and nitrogen fixation efficiency are limited, with improvements of around 30% being achieved at best. Achieving a doubling of nodulation and nitrogen fixation efficiency while also significantly increasing growth (a potential violation of the AON theory) is difficult. Improving the nodulation and nitrogen fixation efficiency of legumes like peanuts and soybeans remains a hotly debated and challenging issue internationally.

[0005] To address these challenges, the inventors' team has conducted over 20 years of continuous research, uncovering the spatiotemporal correlation between the abundance and toxicity of aflatoxin-producing fungi, geography, climate, and 53 other factors, and the occurrence of aflatoxin. They have developed highly sensitive detection and early warning technologies, identified the source of aflatoxin contamination in the soil, constructed a library of aflatoxin-producing strains, and for the first time proposed the scientific concept of coupling soil-source control of aflatoxin with nodulation and nitrogen fixation. By analyzing the microbial population structure within the peanut rhizosphere, isolating and identifying the strains and constructing a library of probiotic strains, and assembling a large number of combinations, they completed laboratory and field screening and identification, thus opening up research and exploration into the coupling of soil-source control of aflatoxin and induced nodulation and nitrogen fixation. Over the past five years, the ARC microbial agent has been successfully developed. This agent effectively controls aflatoxin at the source while simultaneously promoting efficient nodulation and nitrogen fixation in soybeans and peanuts, significantly increasing yields. This microbial agent, known as ARC (Aspergillus flavus / Aflatoxins and Rhizobia Coupling), combines the effects of controlling aflatoxin contamination and promoting nodulation and nitrogen fixation. This agent is simple to use, low-cost, and highly effective. It boasts significant application potential, boasting two fixations (nitrogen and carbon fixation), three increases (increased yield, efficiency, and safety), and five reductions (reduced toxicity, reduced losses, reduced fat, reduced costs, and reduced carbon emissions). Field trials have demonstrated and validated this in numerous major soybean, peanut, pea, and other legume-producing regions nationwide. This is of great significance for boosting my country's soybean oilseed production capacity and promoting green, low-carbon, and efficient production. Summary of the Invention

[0006] In response to the shortcomings of the existing technology, the present invention provides an ARC microbial agent for coupled toxicity control and nitrogen fixation and yield increase, as well as a crop production method. The agent is applied to crop production including leguminous crops such as soybeans and peanuts, achieving coupled toxicity control and nitrogen fixation, quality improvement and yield increase. The agent is simple to use, has significant social and ecological benefits, and is easy to promote and apply.

[0007] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0008] The ARC microbial agent for controlling toxicity and fixing nitrogen coupled with increasing yield is a microbial composition with a coupled effect of controlling toxicity and fixing nitrogen. It has the function of regulating and increasing the abundance of rhizobia in the rhizosphere of leguminous crops and increasing the number of nodules in leguminous crops. It contains all the gene sequences in DNA sequences 1 to 4 shown in SEQ ID No. 1-4.

[0009] According to the above scheme, the ARC microbial agent has an inhibitory effect on Aspergillus flavus and / or its toxins. Furthermore, the inhibition rate of Aspergillus flavus can reach more than 60%, and the inhibition rate of aflatoxin can reach more than 80%. The inhibition of Aspergillus flavus and / or toxins can be detected using other conventional detection methods in the prior art, or the inhibition analysis of Aspergillus flavus and / or its toxins can be performed by inhibition analysis of marker molecules of Aspergillus flavus toxin-producing fungi. Specifically, the inhibition analysis of ARC microbial agent on Aspergillus flavus and / or its toxins can be performed by inhibition analysis of marker molecules of Aspergillus flavus toxin-producing fungi.

[0010] According to the above scheme, the ARC microbial agent of the present invention significantly inhibits the expression of the Aspergillus flavus PAB-01 protein, the amino acid sequence of which is shown in SEQ ID No. 5. The inhibition rate is greater than 90%, preferably greater than 95%, reflecting the excellent antibacterial and antitoxic effects of the ARC microbial agent of the present invention. The method for determining the inhibition rate of Aspergillus flavus PAB-01 protein expression can be referenced in the following literature: Protein biomarker for early diagnosis of microbial toxin contamination: Using Aspergillus flavus as an example, Food Frontiers. 2023, 4, 2013-2023, DOI: 10.1002 / fft2.295.

[0011] According to the above scheme, preferably, the ARC microbial agent is a combination of three or more microorganisms.

[0012] According to the above scheme, the ARC microbial agent has an inhibitory effect on aflatoxin / its toxins.

[0013] According to the above scheme, the ARC microbial agent has an inhibitory effect on one or more pathogens (pathogenic factors) / toxins of soil-borne plant pathogens such as Penicillium, Aspergillus other than Aflatoxin, Fusarium, Sclerotinia, Pseudomonas solanacearum, Phytophthora, Sclerotinia, Pythium, and Rhizoctonia.

[0014] According to the above scheme, the ARC microbial agent can promote early nodulation of leguminous crops such as peanuts and soybeans and prolong the nodulation and nitrogen fixation time.

[0015] The DNA sequence genes shown in SEQ ID No. 1-4 may vary to a certain extent in different strains. When the degree of variation is small, such as no more than 10% base variation, preferably no more than 5% base variation, and more preferably no more than 1% base variation, that is, when the identity is greater than 90%, preferably greater than 95%, and more preferably greater than 99%, and when the corresponding biological activity is present, these are referred to as functional equivalents of the DNA sequences shown in SEQ ID No. 1-4, and containing these sequences is equivalent to containing the corresponding sequences of DNA sequences 1 to 4. Microbial compositions containing the DNA sequences shown in SEQ ID NO. 1 to 4 or their functional equivalents, and having a coupled effect of controlling toxicity and nitrogen fixation, and having the effect of regulating and increasing the abundance of rhizobia in the rhizosphere of leguminous crops and increasing the number of nodules in leguminous crops, are all ARC microbial agents of the present invention.

[0016] DNA sequences 1-4 are specific sequences obtained by comparison with Genbank genome data. They are directly or indirectly related to the function of the microbial agent of this patent. When all the gene sequences of DNA sequences 1-4 are present, they have a coupled effect of controlling toxicity and nitrogen fixation, regulating and increasing the abundance of rhizobia in the rhizosphere of legume crops and increasing the number of nodules in legume crops, thus forming the ARC microbial agent of the present invention. These genes may vary to a certain extent among different strains. If the degree of variation is small, such as no more than 10% base variation, and the corresponding biological activity is maintained, containing them is equivalent to containing the gene sequences shown in SEQ ID Nos. 1-4.

[0017] According to the above scheme, preferably, the above-mentioned ARC microbial agent can be, but is not limited to, a composition of the following four microorganisms: Bacillus laterosporus with a preservation number of CCTCC NO: M 20231815, Bacillus amyloliquefaciens with a preservation number of CCTCC NO: M 20231598, Bacillus mucilaginosus with a preservation number of CCTCC NO: M 20231817, and Enterobacter ludwigii with a preservation number of CCTCC NO: M 20231595.

[0018] Bacillus laterosporus H-CB4802, deposit date is September 27, 2023, deposit number is CCTCC NO: M 20231815, classification name is: Brevibacillus laterosporus strain H-CB4802, deposit unit name is China Center for Type Culture Collection, address is Wuhan University, Wuhan, China.

[0019] Bacillus amylolyticus AR1004, deposited on September 4, 2023, with a deposit number of CCTCC NO: M 20231598, is classified as Bacillus amylolyticus AR1004, and is deposited in China Center for Type Culture Collection, Wuhan University, Wuhan, China.

[0020] Bacillus mucilaginosus JZ2013, deposited on September 27, 2023, with the deposit number CCTCC NO: M 20231817, classified as: Bacillus mucilaginosus strain JZ2013, deposited in China Center for Type Culture Collection, Wuhan University, Wuhan, China.

[0021] Enterobacter ludwigii AR1001, deposit date is September 4, 2023, deposit number is CCTCC NO:M 20231595, classification name is: Enterobacter ludwigii AR1001, deposit unit name is China Center for Type Culture Collection, address is Wuhan University, Wuhan, China.

[0022] According to the above scheme, preferably, the above-mentioned ARC microbial agent can be a combination of one or more microorganisms selected from Bacillus laterosporus with a preservation number of CCTCC NO: M 20231815, Bacillus amyloliquefaciens with a preservation number of CCTCC NO: M 20231598, Bacillus mucilaginosus with a preservation number of CCTCC NO: M 20231817, and Enterobacter ludwigii with a preservation number of CCTCC NO: M 20231595, and other microorganisms, so that the combined microbial agent satisfies the requirements of containing all gene sequences in the nucleotide sequences shown in SEQ ID NOs. 1 to 4 or their functional equivalents, has a coupled effect of toxicity control and nitrogen fixation, and has the effect of regulating and increasing the abundance of rhizobia in the rhizosphere of leguminous crops and increasing the number of nodules in leguminous crops, thereby constituting the ARC microbial agent of the present invention.

[0023] According to the above solution, the proportion of viable bacteria count of any one strain in the above microbial agent, i.e. the mixed microbial composition, is greater than or equal to 1%.

[0024] The ARC microbial agent of the present invention is a microbial composition. The synergistic action of the various microorganisms in the microbial composition produces a coupled effect of toxicity control and nitrogen fixation, exerting a coupled effect of toxicity control and nitrogen fixation. When used in crop production, it has a coupled effect of toxicity control and nitrogen fixation, plays a coupled role in preventing aflatoxin and its toxin pollution and promoting nodulation and nitrogen fixation, and has the function of regulating and increasing the abundance of rhizobia in the rhizosphere of legume crops and increasing the number of nodules in legume crops. Although it is not a rhizobium itself, it can regulate and increase the abundance of rhizobia in the rhizosphere of legume crops, increase the number of nodules in legume crops, and improve the nitrogenase activity of a single plant. It can increase the abundance of rhizobia in the rhizosphere of legume crops by at least 15%, and increase the number of nodules by more than 2 times. It can promote early nodulation of peanuts and soybeans and prolong the time of nodulation and nitrogen fixation.

[0025] The ARC microbial agent can be prepared by the following method: the microorganisms in the above microbial agent are combined and fermented. The fermentation route can adopt conventional fermentation routes of bacteria disclosed in the prior art, including existing literature.

[0026] The above-mentioned ARC microbial agent can be used in the production of leguminous crops as follows: to improve the quality and safety level of leguminous crop products; to promote nodulation and nitrogen fixation of leguminous crops; to increase the yield level of leguminous crops; to recruit indigenous rhizobia and increase the abundance of rhizobia in the rhizosphere soil of leguminous crops; to promote early nodulation of leguminous crops and prolong the nodulation and nitrogen fixation time; to prevent leguminous crops from premature aging due to lack of fertilizer during maturity; to increase the number of leguminous crop pods; to increase the fullness of leguminous crop pods and reduce the rate of shrunken pods; to promote early flowering and early pod formation of leguminous crops; to reduce the occurrence of peanut fruit rot; to reduce the occurrence of bacterial wilt of leguminous crops; to reduce the occurrence of powdery mildew of leguminous crops; to reduce the occurrence of leaf spot of leguminous crops; to reduce the occurrence of root nematode disease of leguminous crops; to reduce Used to reduce the occurrence of root rot in leguminous crops; used to reduce the occurrence of root nematode disease in leguminous crops; used to reduce the occurrence of blight in leguminous crops; used to reduce the occurrence of sclerotinia rot in leguminous crops; used to reduce the occurrence of downy mildew in leguminous crops; used to reduce the occurrence of wilt in leguminous crops; used to reduce the occurrence of white rot in leguminous crops; used to reduce the incidence of soybean green spondylosis; used to reduce the incidence of corn ear rot and Fusarium toxin; used to reduce the abundance of wheat fusarium and reduce the incidence of fusarium toxin; used to promote carbon emission reduction in leguminous crops, which is beneficial to soil improvement; used to promote the increase of total biomass of leguminous crops; used to reduce the abundance of pests such as Aspergillus terreus and Fusarium in the rhizosphere of leguminous crops, which is beneficial to improving the soil microbial population structure; used to reduce the surface spots of peanuts and increase commercial value; used to promote soybean production in saline-alkali land.

[0027] More specifically, it can be used for the following purposes:

[0028] Use of ARC microbial agent to improve peanut quality and safety and reduce losses

[0029] Use of ARC microbial agent to promote peanut nodulation and nitrogen fixation to achieve green and low-carbon production

[0030] Use of ARC microbial agent to increase peanut yield and total biomass

[0031] Application of ARC microbial agent in peanut to prolong the nodulation and nitrogen fixation time and prevent premature aging due to nutrient deficiency

[0032] ARC microbial agent is used to promote early nodulation, nitrogen fixation, flowering, needle formation and fruit setting in peanuts

[0033] ARC microbial agent is used in peanut production to increase the number of pods, improve the plumpness of peanuts and the weight of 100 peanuts.

[0034] Use of ARC microbial agent to reduce fungal diseases such as peanut fruit rot, white rot, root rot and their damage

[0035] Use of ARC microbial agent in reducing bacterial diseases such as peanut wilt and their damage

[0036] Use of ARC microbial agent in reducing peanut root knot nematode disease and its damage

[0037] ARC microbial agent is used to reduce mildew spots and other spots on the surface of peanuts to improve marketability

[0038] Use of ARC microbial agent to improve soybean quality and reduce the risk of aflatoxin contamination

[0039] The use of ARC microbial agent to promote soybean nodulation and nitrogen fixation to achieve green and low-carbon production

[0040] Application of ARC microbial agent in increasing soybean yield and total biomass

[0041] Application of ARC microbial agent in soybean to prolong the nodulation and nitrogen fixation time and prevent premature aging due to nutrient deficiency

[0042] The use of ARC microbial agent for early nodulation, nitrogen fixation, early flowering and pod formation in soybeans

[0043] ARC microbial agent is used to reduce the soybean pod shrinkage rate and increase the soybean pod fullness and 100-grain weight.

[0044] Use of ARC microbial agent in alleviating soybean greening and its hazards

[0045] The use of ARC microbial agent to reduce fungal diseases and damages such as soybean powdery mildew and downy mildew

[0046] Use of ARC microbial agent to reduce soybean root rot and its damage

[0047] Use of ARC microbial agent to shorten soybean pod spacing and increase pod number

[0048] Application of ARC microbial agent in increasing soybean yield in saline-alkali land

[0049] Application of ARC microbial agent in promoting nodulation and nitrogen fixation of leguminous vegetables and increasing yield

[0050] Use of ARC microbial agent to promote nodulation and nitrogen fixation in leguminous grain crops and increase yield

[0051] Use of ARC microbial agent in promoting nodulation and nitrogen fixation of leguminous forage grasses and increasing yield

[0052] The use of ARC microbial agents to promote carbon emission reduction and improve arable land quality in the production of leguminous crops such as soybeans and peanuts

[0053] The use of ARC microbial agent to reduce the harm of continuous cropping of leguminous crops such as peanuts and soybeans

[0054] The use of ARC microbial agent in corn production to control bacteria and reduce toxicity, improve quality and safety, and reduce losses

[0055] ARC microbial agent is used in wheat production to control bacteria and reduce toxicity, improve quality and safety levels and reduce losses.

[0056] The details of each use are as follows:

[0057] Application 1: ARC microbial agent is used to improve peanut quality and safety and reduce losses

[0058] A method for improving peanut quality and safety and reducing losses, comprising applying an ARC microbial agent to peanut production.

[0059] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0060] According to the above scheme, the application method is one or a combination of broadcasting, spraying, drip irrigation, etc., and the application stage is when peanuts are sown and / or from the emergence of peanuts to the flowering and needle-setting period.

[0061] According to the above plan, the above-mentioned ARC microbial agent is mixed with the peanut sowing base fertilizer, and applied evenly into the field by one or more of the following methods: manual, seeding machine, drone, etc. The application rate of the agent is 80 billion to 100 billion live bacteria per mu. After sowing, drip irrigation can be adopted for fields with conditions to avoid severe dryness in the field, uneven emergence, etc., and conventional field management is adopted for other fields.

[0062] The ARC microbial agent can also be applied to peanut production as follows: After normal peanut sowing and emergence, the ARC microbial agent is evenly applied to the field through one or more topdressing methods, such as broadcasting, spraying, and drip irrigation, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Conventional field management is used for all other aspects.

[0063] Use 2: ARC microbial agent is used to promote peanut nodulation and nitrogen fixation to achieve green and low-carbon production

[0064] ARC microbial agent is used to promote peanut nodulation and nitrogen fixation to achieve green and low-carbon production.

[0065] A method for promoting peanut nodulation and nitrogen fixation to achieve green and low-carbon production, comprising applying ARC microbial agent to peanut production.

[0066] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0067] According to the above scheme, the application method is one or a combination of broadcasting, spraying, drip irrigation, etc., and the application stage is when peanuts are sown and / or from the emergence of peanuts to the flowering and needle-setting period.

[0068] According to the above plan, the application method of ARC microbial agent in peanut production can be as follows: mix the above ARC microbial agent with peanut sowing base fertilizer, and apply it evenly to the field by manual means, seed drill or drone, etc. The application rate of the agent is 80 billion to 100 billion live bacteria per mu. After sowing, drip irrigation can be adopted for fields with conditions to avoid severe dryness in the field, uneven emergence, etc., and conventional field management is adopted for other purposes.

[0069] According to the above scheme, the application method of ARC microbial agent in peanut production can also be as follows: After the peanuts are sown and seedlings have emerged normally, and until the flowering and needle setting stage, the above-mentioned microbial agent should be evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, with the application rate of the agent accumulating 80 billion to 100 billion viable bacteria per mu. All other field management methods are the same.

[0070] The application of the above-mentioned ARC microbial agent can significantly promote peanut nodulation and nitrogen fixation, reduce carbon dioxide emissions, and achieve green and low-carbon production.

[0071] Application 3: Use of ARC microbial agent to increase peanut yield and total biomass

[0072] The use of ARC microbial agent for increasing the yield level and total biomass of peanuts.

[0073] A method for increasing peanut yield and total biomass, the method comprising applying an ARC microbial inoculant to peanut production.

[0074] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0075] According to the above scheme, the application method is one or a combination of broadcasting, spraying, drip irrigation, etc., and the application stage is when peanuts are sown and / or from the emergence of peanuts to the flowering and needle-setting period.

[0076] According to the above scheme, the ARC microbial agent can be applied to peanut production by mixing it with a base fertilizer for peanut sowing and evenly applying it to the field using one or a combination of methods, such as manual application, seed drills, or drones, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be used where possible to minimize dryness and uneven seedling emergence. Conventional field management is followed in all other areas.

[0077] The ARC microbial agent can also be applied to peanut production as follows: After normal peanut sowing and emergence, the ARC microbial agent is evenly applied to the field through one or more topdressing methods, such as broadcasting, spraying, and drip irrigation, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Conventional field management is used for all other aspects.

[0078] Use 4: ARC microbial agent is used to extend the nodulation and nitrogen fixation time of peanuts to prevent premature aging due to lack of fertilizer

[0079] ARC microbial agent is used to extend the nodulation and nitrogen fixation time of peanuts to prevent premature aging due to lack of fertilizer.

[0080] The present invention also provides a method for prolonging the nodulation and nitrogen fixation time and preventing premature aging of peanuts, which comprises applying ARC microbial agent to peanut production.

[0081] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0082] According to the above scheme, the application method is one or a combination of broadcasting, spraying, drip irrigation, etc., and the application stage is when peanuts are sown and / or from the emergence of peanuts to the flowering and needle-setting period.

[0083] The application method of the above-mentioned ARC microbial agent in peanut production can be as follows: mix the above-mentioned ARC microbial agent with peanut sowing base fertilizer, and evenly apply it to the field through one or more combinations of manual methods, seed drills, drones, etc. The application rate of the agent is 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0084] The ARC microbial agent can also be applied to peanut production as follows: After normal peanut sowing and emergence, the ARC microbial agent is evenly applied to the field through one or more topdressing methods, such as broadcasting, spraying, and drip irrigation, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Conventional field management is used for all other aspects.

[0085] Use 5 ARC microbial agent is used to promote early nodulation and nitrogen fixation, early flowering, needle formation, and fruit setting in peanuts

[0086] ARC microbial agent is used to promote early nodulation and nitrogen fixation, as well as early flowering, needle formation and fruit setting in peanuts.

[0087] The present invention further provides a method for early nodulation and nitrogen fixation, early flowering, needle formation, and fruit setting in peanuts, which comprises applying an ARC microbial agent to peanut production.

[0088] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0089] According to the above scheme, the application method is one or a combination of broadcasting, spraying, drip irrigation, etc., and the application stage is when peanuts are sown and / or from the emergence of peanuts to the flowering and needle-setting period.

[0090] The application method of the above-mentioned ARC microbial agent in peanut production can be as follows: mix the above-mentioned ARC microbial agent with peanut sowing base fertilizer, and evenly apply it to the field by one or more methods such as manual, seeding machine or drone, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0091] The ARC microbial agent can also be applied to peanut production as follows: After normal peanut sowing and emergence, the ARC microbial agent is evenly applied to the field through one or more topdressing methods, such as broadcasting, spraying, and drip irrigation, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Conventional field management is used for all other aspects.

[0092] Use 6: ARC microbial agent is used in peanut production to increase the number of pods, improve the fullness of peanuts and the weight of 100 peanuts.

[0093] ARC microbial agent is used in peanut production to increase the number of pods, improve the fullness of peanut pods and the weight of 100 peanut kernels.

[0094] The present invention further provides a method for increasing the number of pods, improving the fullness of peanut pods and the weight of 100 peanut pods in peanut production, which comprises applying the ARC microbial agent to peanut production.

[0095] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0096] According to the above scheme, the application method is one or a combination of broadcasting, spraying, drip irrigation, etc., and the application stage is when peanuts are sown and / or from the emergence of peanuts to the flowering and needle-setting period.

[0097] The application method of the above-mentioned ARC microbial agent in peanut production can be as follows: mix the above-mentioned ARC microbial agent with peanut sowing base fertilizer, and evenly apply it to the field by one or more methods such as manual, seeding machine or drone, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0098] The ARC microbial agent can also be applied to peanut production as follows: After normal peanut sowing and emergence, the ARC microbial agent is evenly applied to the field through one or more topdressing methods, such as broadcasting, spraying, and drip irrigation, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Conventional field management is used for all other aspects.

[0099] Use 7 ARC microbial agent is used to reduce fungal diseases such as peanut fruit rot, white rot, root rot and their damage

[0100] ARC microbial agent is used to reduce fungal diseases such as peanut fruit rot, white rot, root rot and their damage.

[0101] A method for reducing fungal diseases such as peanut fruit rot, white rot, and root rot and their damage, comprising applying an ARC microbial agent to peanut crops.

[0102] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0103] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when peanuts are sown and / or before the peanuts become diseased after emergence.

[0104] According to the above scheme, the specific application method of the ARC microbial agent in peanut production is as follows: the ARC microbial agent is mixed with the peanut seeding base fertilizer and evenly applied to the field by one or a combination of manual application, seed drill, or drone, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After application of the ARC microbial agent, the field should be kept dry and uneven seedling emergence should be avoided as much as possible. Otherwise, conventional field management should be followed.

[0105] According to the above scheme, the specific application method of the ARC microbial agent in peanut production can also be as follows: After the peanuts are sown and seedlings emerge normally, until the flowering and needle-setting stage, the ARC microbial agent is evenly applied to the field through one or more topdressing methods such as broadcasting, spraying, and drip irrigation, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. All other conventional field management methods are followed.

[0106] Use 8: ARC microbial agent is used to reduce bacterial diseases such as peanut wilt and their damage

[0107] ARC microbial agent is used to reduce bacterial diseases such as peanut wilt and their damage.

[0108] A method for reducing peanut bacterial wilt disease and its damage, comprising applying an ARC microbial agent to peanut crops.

[0109] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0110] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when peanuts are sown and / or before the peanuts become diseased after emergence.

[0111] According to the above scheme, the ARC microbial agent can be applied to peanut production as follows: The ARC microbial agent is mixed with peanut seeding base fertilizer and evenly applied to the field using one or a combination of methods, such as manual application, seed drill, or drone application, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After application of the ARC microbial agent, the field should be kept dry and uneven seedling emergence should be avoided. Conventional field management should be followed.

[0112] The ARC microbial agent can also be applied to peanut production as follows: After normal peanut sowing and emergence, the ARC microbial agent is evenly applied to the field through one or more topdressing methods, such as broadcasting, spraying, and drip irrigation, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Conventional field management is used for all other aspects.

[0113] Use 9: ARC microbial agent for reducing peanut root knot nematode disease and its damage

[0114] The ARC microbial agent is used to reduce peanut root-knot nematode disease and its damage.

[0115] A method for reducing peanut root-knot nematode disease and its damage, comprising applying an ARC microbial agent to peanut crops.

[0116] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0117] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when peanuts are sown and / or before the peanuts become diseased after emergence.

[0118] The application method of the above-mentioned ARC microbial agent in peanut production can be as follows: mix the above-mentioned ARC microbial agent with peanut sowing base fertilizer, and evenly apply it to the field by one or more methods such as manual, seeding machine or drone, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0119] The ARC microbial agent can also be applied to peanut production as follows: After normal peanut sowing and emergence, the ARC microbial agent is evenly applied to the field through one or more topdressing methods, such as broadcasting, spraying, and drip irrigation, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Conventional field management is used for all other aspects.

[0120] Use 10: ARC microbial agent is used to reduce mildew spots and other spots on the surface of peanuts to improve marketability.

[0121] ARC microbial agent is used to reduce mildew spots and other spots on the surface of peanuts to improve marketability

[0122] A method for reducing mildew spots and other spots on the surface of peanuts and improving marketability, comprising applying an ARC microbial agent to peanut production.

[0123] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0124] According to the above scheme, the application method is one or a combination of broadcasting, spraying, drip irrigation, etc., and the application stage is when peanuts are sown or from the emergence of peanuts to the flowering and needle-setting period.

[0125] According to the above plan, the above-mentioned ARC microbial agent is mixed with the peanut sowing base fertilizer, and applied evenly into the field by one or more of the following methods: manual, seeding machine or drone. The application rate of the agent is 80 billion to 100 billion live bacteria per mu. After sowing, drip irrigation can be adopted for fields with conditions to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other fields.

[0126] The ARC microbial agent can also be applied to peanut production as follows: After normal peanut sowing and emergence, the ARC microbial agent is evenly applied to the field through one or more topdressing methods, such as broadcasting, spraying, and drip irrigation, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Conventional field management is used for all other aspects.

[0127] Application 11: Use of ARC microbial agent to improve soybean quality and reduce the risk of aflatoxin contamination

[0128] The use of ARC microbial agent to improve soybean quality and reduce the risk of aflatoxin contamination.

[0129] A method for improving soybean quality and reducing the risk of aflatoxin contamination, comprising applying an ARC microbial agent to soybean crops.

[0130] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0131] According to the above scheme, the application method is one or a combination of broadcasting, spraying, drip irrigation, etc., and the application stage is when soybeans are sown and / or from soybean emergence to flowering.

[0132] According to the above plan, the above-mentioned ARC microbial agent is mixed with the soybean sowing base fertilizer, and applied evenly into the field by one or more of the following methods: manual, seeding machine, drone, drip irrigation pipe, etc. The application rate of the agent is 80 billion to 100 billion live bacteria per mu. After sowing, drip irrigation can be adopted for fields with conditions to avoid severe dryness in the field, uneven emergence, etc., and conventional field management is adopted for other fields.

[0133] The ARC microbial agent can also be applied to soybean production as follows: After soybeans have been sown and seeded normally, until the flowering and podding stage, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, or a combination thereof, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is followed for all other aspects.

[0134] Use 12: ARC microbial agent is used to promote soybean nodulation and nitrogen fixation to achieve green and low-carbon production

[0135] ARC microbial agent is used to promote soybean nodulation and nitrogen fixation to achieve green and low-carbon production.

[0136] The present invention further provides a method for promoting soybean nodulation and nitrogen fixation to achieve green and low-carbon production, which comprises applying ARC microbial agent to soybean production.

[0137] According to the above scheme, the method includes applying the ARC microbial agent to the soybean crop.

[0138] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0139] According to the above scheme, the application method is broadcasting, spraying, drip irrigation, etc., and the application stage is at soybean sowing and / or after seedling emergence until the flowering and podding stage. The application method of the above-mentioned ARC microbial agent in soybean production can be as follows: the above-mentioned ARC microbial agent is mixed with soybean sowing base fertilizer and evenly applied to the field by one or more methods such as manual application, seed drill, drone, drip irrigation pipe, etc., with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be used if conditions permit to minimize severe field dryness and uneven seedling emergence. Otherwise, conventional field management is used.

[0140] The ARC microbial agent can also be applied to soybean production as follows: After soybeans have been sown and seeded normally, until the flowering and podding stage, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, or a combination thereof, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is followed for all other aspects.

[0141] Application 13: Use of ARC microbial agent to increase soybean yield and total biomass

[0142] The use of ARC microbial agent for increasing soybean yield and total biomass.

[0143] The present invention further provides a method for increasing soybean yield and total biomass, the method comprising applying the ARC microbial inoculant to soybean production.

[0144] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0145] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when soybeans are sown and / or from soybean emergence to flowering.

[0146] The application method of the above-mentioned ARC microbial agent in soybean production can be as follows: mix the above-mentioned ARC microbial agent with soybean sowing base fertilizer, and evenly apply it to the field through one or more combinations of manual methods, seed drills, drones, drip irrigation pipes, etc. The application rate of the agent is 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0147] The ARC microbial agent can also be applied to soybean production as follows: After soybeans have been sown and seeded normally, until the flowering and podding stage, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, or a combination thereof, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is followed for all other aspects.

[0148] Use 14: ARC microbial agent is used to extend the nodulation and nitrogen fixation time of soybeans to prevent premature aging due to lack of fertilizer

[0149] ARC microbial agent is used to prolong the nodulation and nitrogen fixation time of soybeans to prevent premature aging due to lack of fertilizer.

[0150] The present invention also provides a method for prolonging the nodulation and nitrogen fixation time of soybeans to prevent premature aging due to nutrient deprivation, which comprises applying an ARC microbial agent to soybean production.

[0151] The application amount of the ARC microbial agent is not less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0152] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when soybeans are sown and / or from soybean emergence to flowering and podding.

[0153] The application method of the above-mentioned ARC microbial agent in soybean production can be as follows: mix the above-mentioned ARC microbial agent with soybean sowing base fertilizer, and evenly apply it to the field through one or more combinations of manual methods, seed drills, drones, drip irrigation pipes, etc. The application rate of the agent is 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0154] The ARC microbial agent can also be applied to soybean production as follows: After soybeans have been sown and seeded normally, until the flowering and podding stage, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, or a combination thereof, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is followed for all other aspects.

[0155] Use 15 ARC microbial agent is used to promote early nodulation and nitrogen fixation, early flowering and pod formation in soybeans.

[0156] ARC microbial agent is used to promote early nodulation and nitrogen fixation, early flowering and pod formation in soybeans.

[0157] A method for early nodulation, nitrogen fixation, early flowering and pod formation of soybeans, comprising applying an ARC microbial agent to soybean production.

[0158] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0159] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when soybeans are sown or from soybean emergence to flowering.

[0160] The application method of the above-mentioned ARC microbial agent in soybean production can be as follows: mix the above-mentioned ARC microbial agent with soybean sowing base fertilizer, and evenly apply it to the field by one or more methods such as manual labor, seeding machines, drones, drip irrigation pipes, etc. The application rate of the agent is 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0161] The ARC microbial agent can also be applied to soybean production as follows: After soybeans have been sown and seeded normally, the ARC microbial agent is evenly applied to the field through one or more of the following topdressing methods: broadcasting, spraying, or drip irrigation, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is followed for all other aspects.

[0162] Use 16 ARC microbial agent is used to reduce the shrunken pod rate of soybean, and increase the plumpness of soybean pods and the weight of 100 grains. ARC microbial agent is used to reduce the shrunken pod rate of soybean, and increase the plumpness of soybean pods and the weight of 100 grains.

[0163] A method for reducing the soybean pod shrunken rate and improving the soybean pod fullness and 100-grain weight, comprising applying ARC microbial agent to soybean production.

[0164] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0165] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when soybeans are sown and / or from soybean emergence to flowering.

[0166] The application method of the above-mentioned ARC microbial agent in soybean production can be as follows: mix the above-mentioned ARC microbial agent with soybean sowing base fertilizer, and evenly apply it to the field through one or more combinations of manual methods, seed drills, drones, drip irrigation pipes, etc. The application rate of the agent is 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0167] The ARC microbial agent can also be applied to soybean production as follows: After soybeans have been sown and seeded normally, until the flowering and podding stage, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, or a combination thereof, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is followed for all other aspects.

[0168] Use 17: Use of ARC microbial agent to alleviate soybean greening and its hazards

[0169] The ARC microbial agent is used to reduce soybean greening disease and its damage.

[0170] A method for reducing soybean greening disease and its damage, comprising applying ARC microbial agent to soybean crops.

[0171] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0172] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when soybeans are sown and / or before the soybeans become diseased after emergence.

[0173] The application method of the above-mentioned ARC microbial agent in soybean production can be as follows: when sowing soybeans, mix the ARC microbial agent with the soybean sowing base fertilizer, i.e., seed fertilizer, and apply it to the field through a seeder. The ARC microbial agent can also be evenly applied to the soybean roots from the emergence of soybeans to the flowering and podding stage, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu; after the application of the agent, try to avoid severe dryness or flooding in the field. Various other supporting measures can be adopted according to local conditions without special requirements.

[0174] Use 18: ARC microbial agent is used to reduce fungal diseases and damages such as soybean powdery mildew and downy mildew

[0175] ARC microbial agent is used to reduce fungal diseases and damages such as soybean powdery mildew and downy mildew.

[0176] A method for reducing fungal diseases and damages such as powdery mildew and downy mildew of soybean, comprising applying an ARC microbial agent to soybean crops.

[0177] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0178] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when soybeans are sown and / or before the soybeans become diseased after emergence.

[0179] The application method of the above-mentioned ARC microbial agent in soybean production can be as follows: mix the above-mentioned ARC microbial agent with soybean sowing base fertilizer, and evenly apply it to the field through one or more combinations of manual methods, seed drills, drones, drip irrigation pipes, etc. The application rate of the agent is 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0180] The ARC microbial agent can also be applied to soybean production as follows: After soybeans have been sown and seeded normally, until the flowering and podding stage, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, or a combination thereof, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is followed for all other aspects.

[0181] Use 19: Use of ARC microbial agent to reduce soybean root rot and its damage

[0182] The ARC microbial agent is used to reduce soybean root rot and its damage.

[0183] A method for reducing soybean root rot disease and its damage, comprising applying an ARC microbial agent to soybean crops.

[0184] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0185] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when soybeans are sown and / or before the soybeans become diseased after emergence.

[0186] The application method of the above-mentioned ARC microbial agent in soybean production can be as follows: mix the above-mentioned ARC microbial agent with soybean sowing base fertilizer, and evenly apply it to the field through one or more combinations of manual methods, seed drills, drones, drip irrigation pipes, etc. The application rate of the agent is 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0187] The ARC microbial agent can also be applied to soybean production as follows: After soybeans have been sown and seeded normally, until the flowering and podding stage, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, or a combination thereof, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is followed for all other aspects.

[0188] Use 20 ARC microbial agent for shortening soybean pod spacing and increasing pod number

[0189] ARC microbial agent is used to shorten the spacing between soybean pods and increase the number of pods.

[0190] The present invention also provides a method for shortening soybean pod spacing and increasing pod number, which comprises applying ARC microbial agent to soybean production.

[0191] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0192] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when soybeans are sown and / or from soybean emergence to flowering.

[0193] The application method of the above-mentioned ARC microbial agent in soybean production can be as follows: mix the above-mentioned ARC microbial agent with soybean sowing base fertilizer, and evenly apply it to the field through one or more combinations of manual methods, seed drills, drones, drip irrigation pipes, etc. The application rate of the agent is 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0194] The ARC microbial agent can also be applied to soybean production as follows: After soybeans have been sown and seeded normally, until the flowering and podding stage, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, or a combination thereof, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is followed for all other aspects.

[0195] Use 21. Use of ARC microbial agent to increase soybean yield in saline-alkali soil

[0196] The use of ARC microbial agent in increasing soybean yield in saline-alkali soil.

[0197] The present invention provides a method for improving soybean yield in saline-alkali land, which comprises applying an ARC microbial agent to soybean crops.

[0198] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0199] According to the above scheme, the application method is one or a combination of broadcasting, spraying, drip irrigation, etc., and the application stage is when soybeans are sown and / or from soybean emergence to flowering.

[0200] The ARC microbial agent can be applied to soybean production as follows: The ARC microbial agent is mixed with soybean seeding base fertilizer and applied evenly to the field using one or more methods, such as manual application, seed drills, drones, and drip irrigation pipes, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be used where possible to minimize dryness and uneven seedling emergence. Conventional field management is followed for all other fields.

[0201] The ARC microbial agent can also be applied to soybean production as follows: After soybeans have been sown and seeded normally, until the flowering and podding stage, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, or a combination thereof, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is followed for all other aspects.

[0202] Use 22 ARC microbial agent for promoting nodulation and nitrogen fixation in leguminous vegetables and increasing yield

[0203] ARC microbial agent is used to promote nodulation and nitrogen fixation of leguminous vegetables and increase yield.

[0204] The present invention further provides a method for promoting nodulation and nitrogen fixation of leguminous vegetables and increasing yield, which comprises applying ARC microbial agent to leguminous vegetable production.

[0205] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0206] According to the above scheme, the application method is one or more of broadcasting, spraying, and drip irrigation, and the application stage is at the time of sowing of leguminous vegetables and / or from emergence to flowering. The specific application method of the above-mentioned ARC microbial agent in leguminous vegetable production can be as follows: the above-mentioned ARC microbial agent is mixed with the base fertilizer for sowing leguminous vegetables, and evenly applied to the field by one or more of the following methods: manual, seed drill, or drone, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be used in fields where conditions permit, to minimize severe field dryness and uneven emergence. Conventional field management is used for other purposes.

[0207] The specific application method of the ARC microbial agent in leguminous vegetable production can also be as follows: after the leguminous vegetables have been sown and seeded normally, until the flowering stage, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, or a combination thereof, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is followed for all other aspects.

[0208] According to the above scheme, the leguminous vegetables include but are not limited to edible soybeans, peas, broad beans, cowpeas, green beans, sword beans, black-eyed peas, etc.

[0209] Use 23 ARC microbial agent for promoting nodulation and nitrogen fixation in leguminous grain crops and increasing yield

[0210] ARC microbial agent is used to promote nodulation and nitrogen fixation in leguminous grain crops and increase yield.

[0211] The present invention also provides a method for promoting nodulation and nitrogen fixation of leguminous grain crops and increasing yield, which comprises applying an ARC microbial agent to the leguminous grain crops.

[0212] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0213] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when leguminous grain crops are sown and / or from the emergence of peanuts to the flowering stage.

[0214] The specific application method of the above-mentioned ARC microbial agent in the production of leguminous grain crops can be as follows: mix the above-mentioned ARC microbial agent with the base fertilizer for sowing of leguminous grain crops, and apply it evenly to the field by manual means, seeding machines or drones, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0215] The specific application method of the ARC microbial agent in the production of leguminous grain crops can also be as follows: after the leguminous grain crops have been sown and seeded normally, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods until the flowering stage, with the application rate of the agent reaching 80 billion to 100 billion viable bacteria per mu. All other conventional field management methods are followed.

[0216] According to the above scheme, the above-mentioned leguminous grains include but are not limited to red beans, green beans, flower beans, lentils, etc.

[0217] Use 24 of ARC microbial agent for promoting nodulation and nitrogen fixation of leguminous forage grasses and increasing yield

[0218] The use of ARC microbial agent to promote nodulation and nitrogen fixation in leguminous forage crops and increase yield.

[0219] A method for promoting nodulation and nitrogen fixation of leguminous forage and increasing yield, comprising applying an ARC microbial agent to the leguminous forage.

[0220] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0221] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when the leguminous forage grass is sown and / or after emergence and / or after the previous crop is harvested.

[0222] The application method of the above-mentioned ARC microbial agent in the production of leguminous forage can be as follows: the above-mentioned ARC microbial agent is mixed with the sowing base fertilizer of leguminous forage such as alfalfa, and is evenly applied to the field by one or more methods such as manual, seeding machine, drone, drip irrigation pipe, etc., with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and severe dryness of the field and uneven emergence of seedlings can be avoided as much as possible. Conventional field management is adopted for the rest.

[0223] The ARC microbial agent can also be used in leguminous forage production as follows: After alfalfa seeding or previous crop harvest, the ARC microbial agent is evenly applied to the alfalfa field by broadcasting, spraying, drip irrigation, or other topdressing methods, or a combination thereof, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is used for all other purposes.

[0224] According to the above scheme, the above-mentioned legume forage includes alfalfa, Astragalus membranaceus, Sainfoin, clover, Chinese milk vetch, etc.

[0225] Use 25 ARC microbial agent to promote carbon emission reduction in soybean, peanut and other legume crops and improve arable land quality

[0226] ARC microbial agent is used to promote carbon emission reduction in the production of leguminous crops such as soybeans and peanuts and improve the quality of arable land.

[0227] A method for promoting carbon emission reduction in the production of legume crops such as soybeans and peanuts and improving arable land quality, the method comprising applying ARC microbial agent to the production of legume crops such as soybeans and peanuts.

[0228] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0229] According to the above scheme, the application method is one or more of broadcasting, spraying, drip irrigation, etc., and the application stage is when leguminous crops are sown and / or after emergence to flowering and pod setting / needle setting stage.

[0230] According to the above scheme, the legume crops include soybeans, peanuts, red beans, mung beans, peas, broad beans, cowpeas, green beans, kidney beans, alfalfa, and astragalus.

[0231] According to the above plan, the above method is: when sowing leguminous crops or after emergence to flowering and podding / needling stage, ARC microbial agent is evenly applied to crop seeds or around roots, with a dosage of not less than 80 billion viable bacteria per mu. After application, try to avoid severe dryness or flooding in the field. Other various supporting measures can be adopted according to local conditions without special requirements.

[0232] The specific application method can be as follows: mix the above-mentioned ARC microbial agent with the base fertilizer for leguminous crops, and apply it evenly to the field by manual means, seeders, drones, drip irrigation pipes, etc. The application rate of the agent should be more than 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted if conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc. Various other supporting measures can be adopted according to local conditions without special requirements.

[0233] The ARC microbial agent can also be applied to legume crops as follows: After normal seeding and emergence of legumes, and until flowering, podding, and needle setting, the ARC microbial agent can be evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu (approximately 100 million acre). All other conventional field management procedures are followed.

[0234] The amount of base fertilizer used for sowing bean crops in the field using ARC microbial agent is as follows: P and K fertilizers in the base fertilizer are used at conventional amounts, and nitrogen fertilizer is reduced by 20% to 40% compared with the conventional amount.

[0235] The application of the above-mentioned ARC microbial agent can ultimately achieve an increase in yield of more than 6% after reducing nitrogen fertilizer application by 20% to 40%, thereby having the effect of promoting carbon emission reduction in leguminous crop production and protecting arable land and having important uses.

[0236] Use 26 ARC microbial agent is used to reduce the damage caused by continuous cropping of leguminous crops such as peanuts and soybeans

[0237] ARC microbial agent is used to reduce the damage caused by continuous cropping of leguminous crops such as peanuts and soybeans. It is simple to use, has significant social and ecological benefits, and is easy to promote and apply.

[0238] A method for reducing the damage caused by repeated cropping of leguminous crops such as peanuts and soybeans, comprising applying an ARC microbial agent to peanut production.

[0239] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0240] According to the above scheme, the application method is one or more of broadcasting, spraying, drip irrigation, etc., and the application stage is when sowing legume crops such as soybeans and peanuts and / or after the emergence of legume crops such as soybeans and peanuts before the disease occurs.

[0241] According to the above plan, the above-mentioned ARC microbial agent is mixed with the base fertilizer for sowing legume crops such as soybeans and peanuts, and evenly applied to the field by one or more of the following methods: manual, seeding machine or drone. The application rate of the agent is 80 billion to 100 billion live bacteria per mu. After sowing, drip irrigation can be adopted for fields with conditions to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other fields.

[0242] The ARC microbial agent can also be applied to legume production such as soybeans and peanuts as follows: after normal sowing and emergence of legumes such as soybeans and peanuts, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or a combination of one or more of these methods, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is used for all other aspects.

[0243] According to the above scheme, the legume crops include soybeans, peanuts, broad beans, peas, cowpeas, green beans, alfalfa, etc.

[0244] Application 27: ARC microbial agent is used in corn production to control bacteria and reduce toxicity, improve quality and safety, and reduce losses.

[0245] ARC microbial agent is used in corn production to control bacteria and reduce toxicity, improve quality and safety levels and reduce losses.

[0246] A method for controlling bacteria and reducing toxicity, improving quality and safety, and reducing losses in corn production, comprising applying an ARC microbial agent to corn crops.

[0247] According to the above scheme, preferably, the application amount of the ARC microbial agent is 80 billion to 100 billion viable bacteria per mu.

[0248] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when corn is sown and / or before the corn becomes diseased after seedlings emerge.

[0249] The application method of the above-mentioned ARC microbial agent in corn production can be as follows: mix the above-mentioned ARC microbial agent with corn sowing base fertilizer, and evenly apply it to the field by one or more methods such as manual, seeding machine or drone, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0250] The ARC microbial agent can also be applied to corn production as follows: After corn seedlings have emerged normally, and until the silking stage, before disease develops, the ARC microbial agent is evenly applied to the field via one or more topdressing methods, such as broadcasting, spraying, and drip irrigation, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is used for all other aspects.

[0251] Use 28 ARC microbial agent is used in wheat production to control bacteria and reduce toxicity, improve quality and safety levels and reduce losses.

[0252] ARC microbial agent is used in wheat production to control bacteria and reduce toxicity, improve quality and safety levels and reduce losses.

[0253] According to the above scheme, the above-mentioned fungal control and toxin reduction refers to the prevention and control of wheat fusarium rust pathogenic fungi and the reduction and control of vomitoxin and other Fusarium fungal toxins.

[0254] A method for controlling bacteria and reducing toxicity, improving quality and safety, and reducing losses in wheat production, comprising applying an ARC microbial agent to wheat crops.

[0255] According to the above scheme, the application amount of the ARC microbial agent is no less than 80 billion viable bacteria per mu, for example, the application amount is 80 billion to 100 billion viable bacteria, or more than 80 billion to 100 billion viable bacteria.

[0256] According to the above scheme, the application method is one or a combination of broadcasting, spraying, and drip irrigation, and the application stage is when wheat is sown and / or before the wheat seedlings emerge and the disease occurs.

[0257] The application method of the above-mentioned ARC microbial agent in wheat production can be as follows: mix the above-mentioned ARC microbial agent with wheat sowing base fertilizer, and evenly apply it to the field by one or more methods such as manual, seeding machine or drone, with a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. After sowing, drip irrigation can be adopted for fields where conditions permit, and try to avoid severe dryness in the field, uneven emergence of seedlings, etc., and conventional field management is adopted for other purposes.

[0258] The ARC microbial agent can also be applied to wheat production as follows: After wheat seedlings have normally emerged and until the onset of disease at the heading and flowering stage, the ARC microbial agent is evenly applied to the field by broadcasting, spraying, drip irrigation, or other topdressing methods, or a combination thereof, at a cumulative application rate of 80 billion to 100 billion viable bacteria per mu. Conventional field management is followed for all other aspects.

[0259] The third aspect of the present invention provides the use of the above-mentioned ARC microbial agent in compounding with a series of fertilizer products to prepare ARC+ series fertilizer products, which are used in production to simultaneously exert the effects of the ARC microbial agent, specifically including:

[0260] Use of ARC microbial agent in the production of ARC+ macronutrient compound fertilizer

[0261] The use of ARC microbial agent in the production of ARC + medium and trace element compound fertilizer

[0262] Use of ARC microbial agent in the production of ARC+ compound fertilizer / compound fertilizer

[0263] Use of ARC microbial agent in the production of ARC+ organic fertilizer

[0264] Use of ARC microbial agent in the production of ARC+ microbial agent

[0265] Use of ARC microbial agent in the production of ARC+ blended fertilizers

[0266] Use of ARC microbial agent in the production of ARC+ slow-release fertilizer

[0267] Use of ARC microbial agent in the production of ARC+ bio-organic fertilizer

[0268] Use of ARC microbial agent in the production of ARC+ water-soluble fertilizer

[0269] Use of ARC microbial agent in the production of ARC+ organic-inorganic compound fertilizer

[0270] Use of ARC microbial agent in the production of ARC+ rhizobium fertilizer (agent)

[0271] Use of ARC microbial agent in the production of ARC+ compound microbial fertilizer

[0272] Use of ARC microbial agent in the production of ARC+ fertilizer synergist or fertilizer adjuvant

[0273] Use of ARC microbial agent in the production of ARC+ biocontrol agent

[0274] Use of ARC microbial agent in the production of ARC+ soil conditioner

[0275] Use of ARC microbial agent in the production of ARC+ water-retaining agent

[0276] Use of ARC microbial agent in the production of ARC+ pesticide

[0277] The ARC microbial agent is used to produce ARC+ seed dressing agent or seed soaking agent.

[0278] The present invention further provides an ARC+ series of fertilizer products prepared using the above-mentioned ARC microbial agent. The ARC+ series of fertilizer products include ARC+ macro-element compound fertilizers, ARC+ medium and trace element compound fertilizers, ARC+ compound fertilizers / compound fertilizers, ARC+ organic fertilizers, ARC+ microbial agents, production of ARC+ blended fertilizers, ARC+ slow-release fertilizers, ARC+ bio-organic fertilizers, ARC+ water-soluble fertilizers, production of ARC+ organic-inorganic compound fertilizers, ARC+ rhizobium fertilizers (agents), ARC+ compound microbial fertilizers, ARC+ fertilizer synergists or fertilizer adjuvants, ARC+ biocontrol agents, ARC+ soil conditioners, ARC+ water-retaining agents, ARC+ pesticides, ARC+ seed dressing agents or seed dressing agents or seed soaking agents.

[0279] The fourth aspect of the present invention provides a method for preparing ARC+ series fertilizer products using the above-mentioned ARC microbial agent, which specifically comprises:

[0280] (1) The above-mentioned ARC microbial agent is used to produce ARC+macro-element compound fertilizer, and its production method can be as follows: according to the conventional dosage of macro-element fertilizer per mu of land and the dosage of ARC microbial agent with a viable count of not less than 80 billion, the macro-element fertilizer and the ARC microbial agent are proportioned, and then the ARC microbial agent is adsorbed and fixed on the macro-element fertilizer particles by physical methods to prepare the ARC+macro-element compound fertilizer.

[0281] The above-mentioned macro-element fertilizers can be purchased directly from the market, or inorganic fertilizers such as nitrogen fertilizer, phosphorus fertilizer, and potassium fertilizer can be purchased as raw materials and then configured according to common proportions.

[0282] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the macronutrient fertilizer particles for adsorption.

[0283] (2) The above-mentioned ARC microbial agent is used to produce ARC+medium and trace element compound fertilizer, and its production method can be as follows: according to the conventional dosage of medium and trace element compound fertilizer per mu of land and the dosage of ARC microbial agent of not less than 80 billion viable bacteria, ARC+medium and trace element compound fertilizer and ARC microbial agent are proportioned, and then the ARC microbial agent is adsorbed and fixed on the medium and trace element compound fertilizer particles by physical methods to prepare ARC+medium and trace element compound fertilizer.

[0284] The above-mentioned compound fertilizers of medium and trace elements can be purchased directly from the market, or inorganic fertilizers such as nitrogen fertilizer, phosphorus fertilizer, and potassium fertilizer can be purchased as raw materials and then configured according to common proportions.

[0285] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the medium and trace element fertilizer particles for adsorption.

[0286] (3) The above-mentioned ARC microbial agent is used to produce ARC+ compound fertilizer / compound fertilizer, and its production method can be as follows: according to the amount of compound fertilizer / compound fertilizer per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the compound fertilizer / compound fertilizer and the ARC microbial agent are proportioned, and then the ARC microbial agent is adsorbed and fixed on the compound fertilizer / compound fertilizer particles by physical methods to prepare ARC+ compound fertilizer / compound fertilizer.

[0287] The above-mentioned compound fertilizer / compound fertilizer can be purchased from the market, or can be prepared by using inorganic fertilizers such as nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer, etc. as raw materials according to common proportions.

[0288] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the compound fertilizer particles for adsorption.

[0289] (4) The above-mentioned ARC microbial agent is used to produce ARC+ organic fertilizer, and its production method can be as follows: according to the conventional amount of organic fertilizer per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the organic fertilizer and the ARC microbial agent are mixed, and then the ARC microbial agent is adsorbed and fixed on the organic fertilizer particles by physical methods to prepare ARC+ organic fertilizer.

[0290] The above organic fertilizers can be purchased directly from the market, or can be prepared by conventional harmless treatment and composting of animal and plant residues.

[0291] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the organic fertilizer particles for adsorption.

[0292] (5) The above-mentioned ARC microbial agent is used to produce ARC+ microbial agent, and its production method can be as follows: the microbial agent and the ARC microbial agent are proportioned according to the amount of microbial agent per mu of land and the amount of ARC microbial agent with a count of not less than 80 billion viable bacteria, and then the ARC microbial agent and the microbial agent are mixed by conventional physical blending to prepare the ARC+ microbial agent.

[0293] The above-mentioned ARC microbial agent is used to produce ARC+ microbial agent, and its production method can also be as follows: according to the amount of microbial agent per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the bacterial liquid / powder of the microbial agent and the bacterial liquid / powder of the ARC microbial agent are proportioned, and then uniformly mixed by conventional physical method, and then physically adsorbed onto a carrier to prepare the ARC+ microbial agent.

[0294] The above-mentioned microbial agents, such as Bacillus, etc., can be purchased from the market, or can be isolated, identified, screened and fermented from leguminous crop nodules by conventional methods.

[0295] The physical method can use a spray gun to spray a mixture of liquid ARC microbial inoculant and microbial inoculant liquid or a mixture of dry powder ARC microbial inoculant and microbial inoculant powder onto the carrier particles.

[0296] The carrier can be one or a mixture of two or more of carbon powder, charcoal powder, humus, zeolite powder, diatomaceous earth, vermiculite, bentonite, weathered coal, etc.

[0297] (6) The above-mentioned ARC microbial agent is used to produce ARC+ blended fertilizer, and its production method can be as follows: according to the conventional amount of blended fertilizer per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the blended fertilizer and the ARC microbial agent are proportioned, and then the ARC microbial agent is adsorbed and fixed on the blended fertilizer particles by physical methods to prepare ARC+ blended fertilizer.

[0298] The above-mentioned blended fertilizers can be purchased from the market, or can be purchased from inorganic fertilizers such as nitrogen fertilizers, phosphorus fertilizers, and potassium fertilizers and blended according to common proportions.

[0299] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the mixed fertilizer particles for adsorption.

[0300] (7) The above-mentioned ARC microbial agent is used to produce ARC+ slow-release fertilizer, and its production method can be as follows: according to the conventional amount of slow-release fertilizer per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the slow-release fertilizer and the ARC microbial agent are mixed, and then the ARC microbial agent is adsorbed and fixed on the slow-release fertilizer particles by physical methods to prepare ARC+ slow-release fertilizer.

[0301] The above-mentioned slow-release fertilizers can be purchased directly from the market.

[0302] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the slow-release fertilizer particles for adsorption.

[0303] (8) The above-mentioned ARC microbial agent is used to produce ARC+ bio-organic fertilizer, and its production method can be as follows: according to the conventional amount of bio-organic fertilizer per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the bio-organic fertilizer and the ARC microbial agent are mixed, and then the ARC microbial agent is adsorbed and fixed on the bio-organic fertilizer particles by physical methods to prepare ARC+ bio-organic fertilizer.

[0304] The above-mentioned bio-organic fertilizer can be purchased directly from the market, or can be prepared by conventional harmless treatment and composting of microorganisms and animal and plant residues.

[0305] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the bio-organic fertilizer particles for adsorption.

[0306] (9) The above-mentioned ARC microbial agent is used to produce ARC+ water-soluble fertilizer, and its production method can be as follows: according to the amount of water-soluble fertilizer per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the water-soluble fertilizer and the ARC microbial agent are proportioned, and then the ARC microbial agent and the water-soluble fertilizer are mixed by conventional physical blending to prepare ARC+ water-soluble fertilizer.

[0307] The above water-soluble fertilizer can be purchased from the market or prepared by conventional methods.

[0308] (10) The above-mentioned ARC microbial agent is used to produce ARC+ organic-inorganic compound fertilizer, and its production method can be as follows: according to the conventional amount of organic-inorganic compound fertilizer per mu of land and the amount of ARC microbial agent of not less than 80 billion viable bacteria, the organic-inorganic compound fertilizer and the ARC microbial agent are proportioned, and then the ARC microbial agent is adsorbed and fixed on the organic-inorganic compound fertilizer particles by physical methods to prepare ARC+ organic-inorganic compound fertilizer.

[0309] The above organic-inorganic compound fertilizer can be purchased directly from the market, or can be prepared by mixing organic fertilizer and inorganic fertilizer.

[0310] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the organic-inorganic compound fertilizer particles for adsorption.

[0311] (11) The above-mentioned ARC microbial agent is used to produce ARC+ rhizobium fertilizer (agent), and its production method can be as follows: according to the amount of rhizobium fertilizer (agent) per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the rhizobium fertilizer (agent) and the ARC microbial agent are proportioned, and then the ARC microbial agent and the rhizobium fertilizer (agent) are mixed by conventional physical blending to prepare ARC+ rhizobium fertilizer (agent).

[0312] The above-mentioned ARC microbial agent is used to produce ARC+ rhizobium fertilizer (agent), and its production method can also be as follows: according to the amount of rhizobium fertilizer (agent) per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the bacterial liquid / powder of the rhizobium fertilizer (agent) and the bacterial liquid / powder of the ARC microbial agent are proportioned, and then uniformly mixed by conventional physical method, and then adsorbed onto a carrier by physical method to prepare ARC+ rhizobium fertilizer (agent).

[0313] The above-mentioned rhizobium fertilizer (agent), rhizobium liquid and rhizobium powder can be purchased from the market, or can be prepared by separating, identifying, screening and fermenting the nodules of leguminous crops using conventional methods.

[0314] The above physical method can use a spray gun to spray a mixed bacterial liquid consisting of a liquid ARC microbial agent and a rhizobium fertilizer (agent) or a mixed bacterial powder consisting of a dry powder ARC microbial agent and a rhizobium fertilizer (agent) onto the carrier particles.

[0315] The carrier can be one or a mixture of two or more of carbon powder, charcoal powder, humus, zeolite powder, diatomaceous earth, vermiculite, bentonite, weathered coal, etc.

[0316] (12) The above-mentioned ARC microbial agent is used to produce ARC+ compound microbial fertilizer, and its production method can be as follows: according to the conventional amount of compound microbial fertilizer per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the compound microbial fertilizer and the ARC microbial agent are mixed, and then the ARC microbial agent is adsorbed and fixed on the compound microbial fertilizer particles by physical methods to prepare ARC+ compound microbial fertilizer.

[0317] The above-mentioned compound microbial fertilizer can be purchased directly from the market, or can be compounded by microorganisms and plant nutrients.

[0318] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the composite microbial fertilizer particles for adsorption.

[0319] (13) The above-mentioned ARC microbial agent is used to produce ARC+ fertilizer synergist or fertilizer adjuvant, and its production method can be as follows: according to the amount of fertilizer synergist or fertilizer adjuvant per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the fertilizer synergist or fertilizer adjuvant and the ARC microbial agent are proportioned, and then the ARC microbial agent and the fertilizer synergist or fertilizer adjuvant are mixed by conventional physical blending to prepare ARC+ fertilizer synergist or fertilizer adjuvant.

[0320] The above-mentioned ARC microbial agent is used to produce ARC+ fertilizer synergist or fertilizer adjuvant, and its production method can also be as follows: according to the amount of fertilizer synergist or fertilizer adjuvant per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the ARC microbial agent is physically adsorbed onto the fertilizer synergist or fertilizer adjuvant particles to prepare ARC+ fertilizer synergist or fertilizer adjuvant.

[0321] The above-mentioned fertilizer synergists or fertilizer adjuvants can be purchased from the market.

[0322] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the fertilizer synergist or fertilizer adjuvant particles for adsorption.

[0323] (14) The above-mentioned ARC microbial agent is used to produce ARC+ biocontrol agent, and its production method can be as follows: the biocontrol agent and the ARC microbial agent are proportioned according to the amount of biocontrol agent per mu of land and the amount of ARC microbial agent with a count of not less than 80 billion viable bacteria, and then the ARC microbial agent and the biocontrol agent are mixed by conventional physical blending to prepare the ARC+ biocontrol agent.

[0324] The above-mentioned ARC microbial agent is used to produce ARC+ biocontrol agent, and its production method can also be as follows: according to the amount of biocontrol agent per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the bacterial liquid / powder of the biocontrol agent and the bacterial liquid / powder of the ARC microbial agent are proportioned, and then uniformly mixed by conventional physical method, and then physically adsorbed onto a carrier to prepare the ARC+ biocontrol agent.

[0325] The above-mentioned biocontrol agents, such as Bacillus with biocontrol effect, Trichoderma harzianum with biocontrol effect, Beauveria bassiana with biocontrol effect, etc., can be purchased from the market or prepared by isolating, identifying, screening and fermenting from leguminous crop nodules using conventional methods.

[0326] The physical method can use a spray gun to spray a mixed bacterial liquid consisting of liquid ARC microbial agent and biocontrol agent or a mixed bacterial powder consisting of dry powder ARC microbial agent and biocontrol agent onto the carrier particles.

[0327] The carrier can be one or a mixture of two or more of carbon powder, charcoal powder, humus, zeolite powder, diatomaceous earth, vermiculite, bentonite, weathered coal, etc.

[0328] (15) The above-mentioned ARC microbial agent is used to produce ARC+ soil conditioner, and its production method can be as follows: according to the amount of soil conditioner per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the soil conditioner and the ARC microbial agent are proportioned, and then the ARC microbial agent and the soil conditioner are mixed by conventional physical blending to prepare the ARC+ soil conditioner.

[0329] The above-mentioned ARC microbial agent is used to produce ARC+ soil conditioner, and its production method can also be as follows: according to the amount of soil conditioner per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, the ARC microbial agent is physically adsorbed onto the soil conditioner particles to prepare ARC+ soil conditioner.

[0330] The above-mentioned soil conditioners can be purchased from the market.

[0331] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the soil conditioner particles for adsorption.

[0332] (16) The above-mentioned ARC microbial agent is used to produce ARC+ water-retaining agent, and its production method can be as follows: the water-retaining agent and the ARC microbial agent are mixed according to the amount of water-retaining agent per mu of land and the amount of ARC microbial agent with a viable count of not less than 80 billion, and then the ARC microbial agent is adsorbed and fixed on the water-retaining agent particles by conventional physical methods to prepare ARC+ water-retaining agent.

[0333] The above-mentioned water-retaining agent can be purchased from the market.

[0334] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the water retaining agent particles for adsorption.

[0335] (17) The above-mentioned ARC microbial agent is used to produce ARC+ pesticide, and its production method can be as follows: the pesticide and the ARC microbial agent are proportioned according to the amount of pesticide per mu of land and the amount of ARC microbial agent with a count of not less than 80 billion viable bacteria, and then the ARC microbial agent and the pesticide are mixed by conventional physical blending to prepare ARC+ pesticide.

[0336] The above pesticides can be purchased from the market.

[0337] (18) The above-mentioned ARC microbial agent is used to produce ARC+seed dressing agent or seed mixing agent or seed soaking agent, and its production method can be as follows: according to the amount of seed dressing agent or seed mixing agent or seed soaking agent per mu of land and the amount of ARC microbial agent not less than 80 billion viable bacteria, the seed dressing agent or seed mixing agent or seed soaking agent and the ARC microbial agent are proportioned, and then the ARC microbial agent and the seed dressing agent or seed mixing agent or seed soaking agent are mixed by conventional physical blending to prepare ARC+seed dressing agent or seed mixing agent or seed soaking agent.

[0338] The above-mentioned seed coating agent, seed dressing agent or seed soaking agent can be purchased from the market.

[0339] A fifth aspect of the present invention provides the use of the ARC+ series fertilizer products prepared using the above-mentioned ARC microbial agent in crop production.

[0340] According to the above scheme, the crops include but are not limited to leguminous crops, corn, and wheat. The leguminous crops include peanuts, soybeans, and leguminous grains such as red beans and mung beans; leguminous vegetables such as peas, broad beans, cowpeas, French beans, and kidney beans; and leguminous forage crops such as alfalfa and milk vetch.

[0341] A sixth aspect of the present invention provides a method for crop production, wherein a microbial agent is selected so that the microbial agent contains all the gene sequences in the nucleotide sequences shown in SEQ ID NOs. 1 to 4 after analysis and determination, and has a coupled effect of controlling toxicity and fixing nitrogen, and has the effect of regulating and increasing the abundance of rhizobia in the rhizosphere of leguminous crops and increasing the number of nodules in leguminous crops; the microbial agent is applied to crops to control toxicity and fix nitrogen, improve quality and increase yield.

[0342] According to the above scheme, the DNA sequence genes shown in SEQ ID No. 1-4 may have a certain degree of variation in different strains. When the degree of variation is small, such as no more than 10% base variation, preferably no more than 5% base variation, and more preferably no more than 1% base variation, that is, the identity is greater than 90%, preferably greater than 95%, and more preferably greater than 99%, and when the corresponding biological activity is present, these are functional equivalents of the DNA sequences shown in SEQ ID No. 1-4, and containing these sequences is equivalent to containing the corresponding sequences of DNA sequences 1 to 4. When containing the gene sequences shown in SEQ ID NO. 1 to 4 or their functional equivalents and having the coupled effect of controlling toxicity and nitrogen fixation as described in the ARC microbial agent, and having the function of regulating and increasing the abundance of rhizobia in the rhizosphere of leguminous crops and increasing the number of nodules in leguminous crops, they are all ARC microbial agents for controlling toxicity and nitrogen fixation coupled with increasing yield of the present invention, and can be used in crop production to promote crop toxicity control and nitrogen fixation, improve quality and increase yield.

[0343] In the above solution, the ARC microbial agent has an inhibitory effect on Aspergillus flavus and / or its toxins. Furthermore, the inhibition rate of Aspergillus flavus can reach more than 60%, and the inhibition rate of aflatoxin can reach more than 80%.

[0344] In the above scheme, the ARC microbial agent significantly inhibits the expression of the Aspergillus flavus PAB-01 protein, the amino acid sequence of which is shown in SEQ ID No. 5. The inhibition rate is greater than 90%, preferably greater than 95%, demonstrating the excellent bacteriostatic and toxicity-reducing effects of the ARC microbial agent of the present invention. According to the above scheme, the ARC microbial agent is preferably a combination of three or more microorganisms.

[0345] In the above scheme, the ARC microbial agent has an inhibitory effect on one or more pathogens (pathogenic factors) / toxins of soil-borne plant pathogens such as Penicillium, Aspergillus other than Aspergillus flavus, Fusarium, Sclerotium sclerotiorum, Pseudomonas solanacearum, Phytophthora, Sclerotinia sclerotiorum, Pythium, and Rhizoctonia solani.

[0346] In the above scheme, the ARC microbial agent can be, but is not limited to, a composition of the following four microorganisms: Bacillus laterosporus with a preservation number of CCTCC NO: M 20231815, Bacillus amyloliquefaciens with a preservation number of CCTCC NO: M 20231598, Bacillus mucilaginosus with a preservation number of CCTCC NO: M 20231817, and Enterobacter ludwigii with a preservation number of CCTCC NO: M 20231595.

[0347] Preferably, the ARC microbial agent described in the above scheme can be a combination of one or more strains of Bacillus laterosporus with a deposit number of CCTCC NO: M 20231815, Bacillus amyloliquefaciens with a deposit number of CCTCC NO: M 20231598, Bacillus mucilaginosus with a deposit number of CCTCC NO: M20231817, and Enterobacter Ludwigii with a deposit number of CCTCC NO: M 20231595, and other microorganisms, so that the combined microbial agent satisfies the requirements of containing all gene sequences in the nucleotide sequences shown in SEQ ID NOs. 1 to 4 or their functional equivalents, has a coupled effect of toxic control and nitrogen fixation, has the effect of regulating and increasing the abundance of rhizobia in the rhizosphere of legume crops, and increasing the number of nodules of legume crops, constituting the ARC microbial agent of the present invention.

[0348] In the above embodiment, the proportion of viable bacteria count of any one strain in the above microbial agent, i.e., the mixed microbial composition, is greater than or equal to 1%.

[0349] The beneficial effects of the present invention are:

[0350] 1. The ARC microbial agent for toxin control and nitrogen fixation coupled with yield increase is used in crop production. Through a single technology, it achieves green control of aflatoxin sources while inducing and promoting efficient nodulation and nitrogen fixation and extremely significant yield increase in legume crops such as soybeans and peanuts. 2. It is easy to use, low cost and high benefit. 3. It is of great significance to promote the expansion of soybean oil production capacity and green, low-carbon and efficient production in my country. DETAILED DESCRIPTION

[0351] Part I ARC Microbial Agents

[0352] Example 1 Preparation of ARC microbial agent

[0353] Whole plants of peanut, soybean, pea, broad bean, cowpea, and alfalfa, along with rhizosphere soil samples, were ground and mixed. Strains were isolated using conventional bacterial isolation methods and subsequently identified using conventional 16S rDNA analysis. Bacillus amyloliquefaciens, Brevibacillus laterosporus, Bacillus mucilaginosus, and Enterobacter ludwigii were obtained through these procedures. Details are shown in Table 1.

[0354] Table 1. Some of the strains isolated and identified from a mixture of major legume crops such as peanuts and soybeans are shown below:

[0355] The 10 microbial strains in Table 1 were amplified one by one by conventional bacterial culture medium amplification method to prepare batches of fermentation broth or bacterial powder of the 10 strains.

[0356] The combination information of the microbial agents formed by mixing the fermentation broth or bacterial powder of the four strains of Bacillus laterosporus with the above-mentioned deposit number CCTCC NO: M 20231815, Bacillus amyloliquefaciens with the deposit number CCTCC NO: M 20231598, Bacillus mucilaginosus with the deposit number CCTCC NO: M 20231817, and Enterobacter Ludwigii with the deposit number CCTCC NO: M 20231595; the combination information of the microbial agents formed by mixing some strains of Bacillus laterosporus with the deposit number CCTCC NO: M 20231815, Bacillus amyloliquefaciens with the deposit number CCTCC NO: M 20231598, Bacillus mucilaginosus with the deposit number CCTCC NO: M 20231817, and Enterobacter Ludwigii with other strains; and some other strains is shown in Table 2, wherein the proportion of the viable cell count of any one strain in each microbial combination is greater than or equal to 1%.

[0357] Table 2. Microbial composition and bacterial agent information

[0358] Example 2: Sequencing of ARC microbial agents

[0359] A sufficient number of samples were taken from the microbial agents in Table 2 of Example 1, and total DNA was extracted from these samples in sequence using a conventional DNA extraction method. The DNA sequences of these samples were then determined using a conventional DNA sequencing method. Finally, conventional analysis methods were used to compare the homology of the DNA sequences determined above with the gene sequences shown in SEQ ID No. 1-4 provided in this patent text.

[0360] The results of the above determination and homology analysis showed that bacterial agents 1 to 4 in Table 2 contained all four genes in the DNA sequences 1 to 4 shown in SEQ ID No. 1 to 4, respectively, and the sequence homology was 100%; bacterial agents 5 to 12 in Table 1 contained all four genes in the DNA sequences 1 to 4 shown in SEQ ID No. 1 to 4, respectively, but with certain variations, and the four gene sequences had 90.3-100% homology with DNA sequences 1 to 4.

[0361] Example 3: Determination of the Toxic Control and Nitrogen Fixation Effect of Peanut ARC Microbial Agent

[0362] Taking peanuts as an example, the steps for determining the toxicity control and nitrogen fixation effects of the above-mentioned ARC microbial agent are described as follows.

[0363] On the one hand, the bacterial agent described in Table 2 of Example 1 was co-cultured with a toxin-producing Aspergillus flavus strain under the same conditions. The expression level of PAB-01 was measured, and the inhibition rate of PAB-01 expression by the bacterial agent was calculated. The results are shown in Table 3. The method for determining the inhibition rate of Aspergillus flavus protein expression can be referred to the method in the following literature: Protein biomarker for early diagnosis of microbial toxin contamination: Using Aspergillus flavus as an example, Food Frontiers. 2023, 4, 2013-2023, DOI: 10.1002 / fft2.295.

[0364] On the other hand, microbial agents 1-12 from Table 2 of Example 1 were applied to the field along with the peanut sowing base fertilizer, or during the peanut growing season, at a cumulative application rate of 80 billion viable bacteria per mu (approximately 100 million). A control plot was established without any of the microbial agents, while all other plots were managed using conventional field management. Root nodulation of peanuts in the seedling stage was investigated 7 to 12 days after emergence; root nodulation of peanuts in the mature stage was investigated 1 to 3 days before harvest; and the effectiveness of toxicity control was investigated from the flowering stage to around harvest. Specifically, the effectiveness of field disease control and the abundance of aflatoxin in peanuts after harvest were investigated. The results of these investigations are shown in Table 3.

[0365] According to the above results, microbial agents 1 to 12 simultaneously possess the following characteristics: 1) The microbial agents contain all four gene sequences in DNA sequences 1 to 4, and these genes may have no more than 10% base variation in different strains; 2) The microbial agents have a significant inhibitory effect on the expression of Aspergillus flavus PAB-01 protein, have an inhibitory effect on Aspergillus flavus, and have an inhibitory effect on one or more pathogenic factors / toxins in soil-borne plant pathogens such as Penicillium and Aspergillus other than Aspergillus flavus, Fusarium, and Pseudomonas solanacearum; 3) Although not a rhizobium itself, the ARC microbial agent can simultaneously regulate and increase the abundance of rhizobia in the peanut rhizosphere and increase the number of peanut root nodules; 4) It can promote early nodulation of peanuts and prolong the time of nodulation and nitrogen fixation.

[0366] The above-mentioned soil-borne plant pathogens are the pathogenic microorganisms of the above-mentioned corresponding field diseases. Therefore, the field disease prevention and control effect is equivalent to the inhibitory effect of ARC microbial agent on the pathogenic factors / toxins of the above-mentioned soil-borne plant pathogens.

[0367] Table 3. Results of the determination of the effect of microbial agents on peanut toxicity control and nitrogen fixation

[0368] The above-mentioned microbial agent has an inhibition rate of more than 60% on aflatoxin / toxin, and further more than 90%; the inhibition rate of one or more pathogenic factors / toxins of soil-borne plant pathogens such as Aspergillus, Penicillium, Fusarium, Pseudomonas solanacearum, etc. other than Aspergillus flavus can reach at least 30% or more, preferably more than 60%, and more preferably 80% or even more than 90%.

[0369] Example 4: Determination of the Toxic Control and Nitrogen Fixation Effect of Soybean Microbial Agents

[0370] Taking soybean as an example, the steps for determining the toxicity control and nitrogen fixation effects of the above-mentioned microbial agents are described as follows.

[0371] On the one hand, the bacterial agent in Table 2 of Example 1 above was co-cultured with the toxigenic Aspergillus flavus strain under the same conditions, the expression level of PAB-01 was determined using the literature method, and the inhibition rate of the bacterial agent on PAB-01 expression was calculated. The results are shown in Table 3.

[0372] On the other hand, microbial agents 1-12 from Table 2 of Example 1 were applied to the field along with soybean sowing base fertilizer, or during the soybean growing season, at a cumulative application rate of 80 billion viable bacteria per mu (approximately 100 million). A control plot was established without any of the microbial agents. Conventional field management was used for all other plots. Root nodulation of soybean seedlings was investigated 7 to 12 days after emergence; root nodulation of peanuts was investigated 1 to 3 days before harvest; and toxicity control efficacy, i.e., effectiveness in preventing and controlling pest toxin-induced diseases, was investigated from the flowering stage to harvest. The results are shown in Table 4.

[0373] Based on the above results, agents 1 to 12 simultaneously possess the following characteristics: 1) ARC microbial agent contains all four gene sequences in DNA sequences 1 to 4, and these genes may have no more than 10% base variation in different strains; 2) the agent has a significant inhibitory effect on the expression of Aspergillus flavus PAB-01 protein, has an inhibitory effect on Aspergillus flavus, and has an inhibitory effect on soil-borne plant pathogens other than Penicillium and Aspergillus flavus, Fusarium, Sclerotinia sclerotiorum, Phytophthora, Sclerotinia sclerotiorum, Pythium, and Rhizoctonia solani; 3) although it is not a rhizobium itself, the agent can simultaneously regulate and increase the abundance of rhizobia in the soybean rhizosphere and the number of soybean root nodules; 4) the agent can promote early soybean nodulation and prolong the time of nodulation and nitrogen fixation.

[0374] The above-mentioned soil-borne plant pathogens are the pathogenic microorganisms of the above-mentioned corresponding field diseases. Therefore, the field disease prevention and control effect is equivalent to the inhibitory effect of ARC microbial agent on the pathogenic factors / toxins of the above-mentioned soil-borne plant pathogens.

[0375] Table 4. Results of the test on the effect of microbial agents on soybean toxicity control and nitrogen fixation

[0376] The above-mentioned bacterial agent has an inhibition rate of more than 60% on aflatoxin / toxin, and further more than 90%; the inhibition rate of one or more pathogenic factors / toxins of soil-borne plant pathogens such as Aspergillus, Fusarium, Sclerotinia, Phytophthora, Sclerotinia, Pythium, and Rhizoctonia other than Aspergillus flavus can reach at least 30% or more, preferably more than 60%, and more preferably 80% or even more than 90%.

[0377] Example 5: Determination of the Effect of Microbial Agents on Toxic Control and Nitrogen Fixation in Other Leguminous Crops

[0378] The sampling was carried out in the same manner as in Examples 3 and 4 to determine the toxicity control and nitrogen fixation effects of ARC microbial agents 1 to 12 on other leguminous crops such as peas, broad beans, cowpeas, and alfalfa, and similar results to those in Tables 3 and 4 were obtained.

[0379] Use of ARC microbial agent to improve peanut quality and safety and reduce losses

[0380] Example 6: Application of ARC Microbial Agents - Improving the Quality and Safety of Leguminous Crop Products and Reducing Losses

[0381] The above-mentioned ARC microbial agents 1 to 8 were mixed with peanut seeding base fertilizer respectively and applied to the field by a seed drill. The application rate of the microbial agents was 80 billion to 100 billion viable bacteria per mu. At the same time, a plot without any of the above microbial agents was set up as a control. The others were managed by conventional field management. After the above-mentioned peanuts were harvested, peanut samples were collected using the national standard sampling method. (1) The abundance of aflatoxin-producing fungi in these samples was determined by the classic colony counting method, and the reduction rate of the abundance of aflatoxin-producing fungi in peanuts was calculated, which is the control effect of aflatoxin-producing fungi; (2) The resveratrol, protein, and amino acid content of peanuts were determined by standard methods, and the quality improvement level was calculated; (3) After the samples were placed under the same conditions for 6 months, the aflatoxin contamination level was determined by the national standard liquid chromatography-mass spectrometry method, and the control effect of aflatoxin was calculated. These test results showed that: (1) the resveratrol, protein, glutamic acid and other quality contents of peanut samples in the fields treated with microbial agents 1 to 8 were significantly improved; (2) the control effect of microbial agents 1 to 8 on aflatoxin-producing fungi carried by field peanuts was above 61.5%, which greatly reduced the risk of aflatoxin contamination in post-harvest peanuts; (3) after the peanut samples in the fields treated with microbial agents 1 to 8 and the control were placed under the same conditions for 6 months, the aflatoxin content of peanuts in the treated groups decreased by more than 82% compared with the control group. Among them, the number of peanut samples in the control group with aflatoxin content exceeding the national limit standard accounted for more than 17%, while the number of peanut samples in the treated groups with aflatoxin content exceeding the national limit standard did not exceed the national limit standard, thereby reducing the peanut losses caused by aflatoxin contamination. The above results show that the application of ARC microbial agents significantly improved the quality and quality safety level of field peanuts and reduced peanut losses.

[0382] The above-mentioned ARC microbial agents were applied in peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar effects of improving quality and quality safety levels were achieved.

[0383] Applying the above-mentioned ARC microbial agents from the time peanuts emerge to the time they bloom and needle drop also achieved similar results in improving quality and quality safety levels.

[0384] Use of ARC microbial agent to promote peanut nodulation and nitrogen fixation to achieve green and low-carbon production

[0385] Example 7: Use of the method for promoting peanut nodulation and nitrogen fixation to achieve green and low-carbon production

[0386] ARC microbial agents 1-8 were mixed with peanut seeding fertilizer and applied to the field via seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was established without any of the agents, while all others were managed using conventional field management. Continuous investigations of peanut root nodulation began after the seedlings emerged. These findings showed that during the flowering period, the number of root nodules in peanuts treated with agents 1-8 increased by more than threefold, and the nitrogenase activity of individual peanut plants increased by more than fivefold. Continuous monitoring of carbon dioxide concentrations in the treated and control plots using a carbon dioxide infrared monitor revealed a reduction of more than 9.9% in carbon dioxide emissions. These results indicate that the application of ARC microbial agents significantly promoted field peanut nodulation and nitrogen fixation, as well as green, low-carbon production.

[0387] The above-mentioned ARC microbial agents were applied in peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar significant effects were achieved in promoting peanut nodulation and nitrogen fixation to achieve green and low-carbon production.

[0388] Applying the above-mentioned ARC microbial agents from the time peanut seedlings emerge to the time when they flower and needle drop also achieved similar significant effects in promoting peanut nodulation and nitrogen fixation to achieve green and low-carbon production.

[0389] Use of ARC microbial agent to increase peanut yield and total biomass

[0390] Example 8: ARC microbial agent is used to increase peanut yield

[0391] ARC microbial agents 1-8 were mixed with peanut seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was established without any of the agents, while all other plots were managed using conventional methods. At harvest time, peanuts were harvested from one mu of land in each of the treatments and controls. Calculations showed that the yield increase in the fields treated with agents 1-8 was over 19%, achieving a significant yield increase. These results demonstrate that the application of ARC microbial agents significantly increased field peanut yields.

[0392] The above-mentioned ARC microbial agents were applied in peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar significant results were achieved in increasing peanut yield.

[0393] Applying the above-mentioned ARC microbial agents from the time peanut seedlings emerge to the time when they bloom and needle drop also achieved similar significant results in increasing peanut yield.

[0394] Example 9: Use for increasing the total biomass of peanuts

[0395] The ARC microbial agents 1-8 were mixed with peanut seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was set up without any of the above agents, while all other plots were managed using conventional field management. A survey was conducted at harvest time to calculate the total biomass of the treated and control peanut plants. These survey results showed that the total biomass of the peanut plants treated with ARC microbial agents 1-8 increased by more than 16%. These results indicate that the application of ARC microbial agents significantly promotes the increase in the total biomass of peanut plants.

[0396] The above-mentioned ARC microbial agents were applied to peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar results were achieved that significantly increased the total biomass of peanuts.

[0397] Applying the above-mentioned ARC microbial agents from the time peanuts emerge to the time they bloom and needle set also achieved similar results in significantly increasing the total biomass of peanuts.

[0398] Application of ARC microbial agent in peanut to prolong the nodulation and nitrogen fixation time and prevent premature aging due to nutrient deficiency

[0399] Used for promoting peanuts to prolong the nodulation and nitrogen fixation time and prevent premature aging due to nutrient deficiency

[0400] Example 10: The above-mentioned ARC microbial agents 1 to 8 were mixed with peanut sowing base fertilizer respectively, and applied to the field through a seeder. The application rate of the agents was 80 billion to 100 billion viable bacteria per mu. At the same time, a plot without application of any of the above-mentioned agents was set as a control, and the others were managed using conventional field management.

[0401] Continuous investigations of peanut root nodulation began after seedling emergence. These results showed that peanuts treated with ARC microbial agents 1-8 developed root nodules approximately 8 days after seedling emergence, more than 20 days earlier than the conventional estimate of 30 days. New nodules were still found during the full-fruiting stage, with the coexistence of old, middle-aged, and young nodules, as well as young and young nodules. Leaf photosynthesis measurements were conducted 2-3 days before harvest, revealing a photosynthetic rate of over 36.5% higher in the ARC-treated group than in the control group. Fresh, viable nodules with nitrogenase activity were still found at harvest, defying the conventional wisdom that no new nodules form after the seed-filling stage and that all nodules decay by harvest. This significantly prolonged the period of nodulation and nitrogen fixation, effectively preventing premature aging due to nutrient depletion. Consequently, peanut yield per unit area increased by over 18.6%. These results demonstrate that the application of ARC microbial agents has achieved earlier nodulation and prolonged the period of nodulation and nitrogen fixation in peanut legumes, preventing premature aging due to nutrient depletion and contributing to higher peanut yields.

[0402] The above-mentioned ARC microbial agents were applied in peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar significant effects were achieved in promoting the extension of peanut nodulation and nitrogen fixation time, preventing premature aging due to nutrient deficiency, and increasing yield.

[0403] Applying the above-mentioned ARC microbial agents from the time peanuts emerge to the time they flower and needle set also achieved similar significant effects in promoting the nodulation and nitrogen fixation of peanuts, preventing premature aging due to lack of fertilizer, and increasing yield.

[0404] ARC microbial agent is used to promote early nodulation, nitrogen fixation, flowering, needle formation and fruit setting in peanuts

[0405] Used to promote early nodulation, flowering, needle formation and fruit setting in peanuts

[0406] Example 11: The aforementioned ARC microbial agents 1-8 were mixed with peanut seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A plot not treated with any of the aforementioned agents was set up as a control, while all other plots were managed using conventional field management. After the peanuts emerged, surveys were conducted continuously, and the flowering and pod setting times of the peanuts compared with the control were statistically analyzed. These survey results showed that peanuts treated with ARC microbial agents 1-8 had nodulation and nitrogen fixation advanced by at least 17 days, and flowering, pod formation, and fruit setting were all advanced by at least 3 days, resulting in an increase in yield per unit area by more than 18%. These results demonstrate that the application of ARC microbial agents significantly promotes earlier flowering, pod formation, and fruit setting in peanuts, contributing to an increase in peanut yield per unit area.

[0407] The above-mentioned ARC microbial agents were applied to peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar significant effects were achieved in promoting early flowering, needle setting, fruit setting and increasing yield of peanuts.

[0408] Applying the above-mentioned ARC microbial agents from the time peanut seedlings emerge to the time when they bloom and set needles also achieved similar significant effects in promoting early flowering, setting needles, and fruiting as well as increasing yield per unit area.

[0409] ARC microbial agent is used in peanut production to increase the number of pods, improve the plumpness of peanuts and the weight of 100 peanuts.

[0410] Example 12: Use in peanut production to increase the number of pods, improve the plumpness and 100-pearl weight of peanuts

[0411] ARC microbial agents 1-8 were mixed with peanut seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Plots not treated with any of the agents served as controls, while all other plots were managed using conventional field management. At harvest, the peanut pods were surveyed and the shriveled pod rate was calculated. These survey results showed that peanuts treated with agents 1-8 had an average pod count increase of at least four pods per plant, significantly improved pod plumpness, reduced shriveled pod rate by over 8%, and increased 100-kernel weight by over 6.3%, ultimately increasing yield per unit area by over 19.5%. These results demonstrate that the application of ARC microbial agents significantly increases peanut pod plumpness, reduces shriveled pod rate, and increases 100-kernel weight, significantly boosting peanut yield per unit area.

[0412] The above-mentioned ARC microbial agents were applied to peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar effects of significantly increasing the number of peanut pods, improving the fullness and 100-grain weight of peanut fruits, and increasing the yield per unit area were achieved.

[0413] Applying the above-mentioned ARC microbial agents from the time peanut seedlings emerge to the time when they bloom and needle drop also achieved similar results in significantly increasing the number of peanut pods, improving the fullness and 100-grain weight of peanut fruits, and increasing the yield per unit area.

[0414] Use of ARC microbial agent to reduce fungal diseases such as peanut fruit rot, white rot, root rot and their damage

[0415] Example 13: Inhibition experiment of ARC microbial agent on peanut fruit rot pathogens

[0416] 5g of ARC microbial agent was mixed with a small amount of the peanut fruit rot pathogen and inoculated into 50mL of LB liquid medium. Simultaneously, the peanut fruit rot pathogen was inoculated into the LB liquid medium without ARC. After incubation on a shaker at room temperature for 3 days, the mycelium was collected, blotted dry with absorbent paper, and weighed. The inhibition rate of ARC microbial agent against the peanut fruit rot pathogen was calculated. The results showed that the inhibition rate of ARC microbial agent against the peanut fruit rot pathogen was above 90%.

[0417] Example 14: Use of ARC microbial agent to alleviate peanut fruit rot

[0418] ARC microbial agents 1-8 were mixed with peanut seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Plots not treated with any of the above agents served as controls, while all other plots were managed using conventional field management. Each experiment was replicated three times. At harvest, the incidence of fruit rot in the treated and control peanuts was analyzed. These findings showed that the incidence of fruit rot in peanuts treated with ARC microbial agents 1-8 was reduced by over 63% compared to the control group, and that treatment with ARC microbial agents restored over 30% of peanut yield losses. These results demonstrate that the application of ARC microbial agents significantly reduces peanut fruit rot, thereby reducing peanut yield losses caused by fruit rot.

[0419] The above-mentioned ARC microbial agents were applied in peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar significant effects of reducing peanut fruit rot and its damage were achieved.

[0420] Applying the above-mentioned ARC microbial agents from the time peanuts emerge to the time they bloom and needle set also achieved similar significant effects in reducing peanut fruit rot and its damage.

[0421] Compared with other existing schemes, the ARC microbial agent has a more prominent preventive effect on peanut fruit rot before the onset of the disease.

[0422] Example 15: Inhibition experiment of ARC microbial agent on peanut white rot pathogen

[0423] 5g of ARC microbial agent was mixed with a small amount of the peanut pathogenic fungus Sclerotium rot and inoculated into 50mL of LB liquid medium. Simultaneously, the peanut pathogenic fungus was inoculated into the LB liquid medium without ARC microbial agent. After incubation in a shaker at room temperature for 3 days, the mycelium was collected, blotted dry with absorbent paper, and weighed. The inhibition rate of ARC microbial agent against the peanut pathogenic fungus was calculated. The results showed that the inhibition rate of ARC microbial agent against the peanut pathogenic fungus was above 93%.

[0424] Example 16: Use of ARC microbial agent to alleviate peanut white rot

[0425] ARC microbial agents 1-8 were mixed with peanut seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 100 million acre). Plots not treated with any of the agents served as controls, while all other plots were managed using conventional field management. After the peanuts emerged, ongoing surveys were conducted to assess the incidence of white rot in the treated and control plots. These survey results showed that the incidence of white rot in peanuts treated with ARC microbial agents 1-8 was reduced by over 60% compared to the control group, and that treatment with ARC microbial agents restored over 30% of peanut yield losses. These results demonstrate that the application of ARC microbial agents significantly reduces the incidence of white rot in peanuts, thereby alleviating peanut yield losses caused by white rot.

[0426] The above-mentioned ARC microbial agents were applied in peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar significant effects of reducing peanut white rot and its damage were achieved.

[0427] Applying the above-mentioned ARC microbial agents after peanut seedlings emerge and before the onset of white rot also achieved similar significant effects in reducing peanut white rot and its damage.

[0428] Compared with other existing schemes, the ARC microbial agent has a more prominent preventive effect on peanut white rot before the onset of the disease.

[0429] Example 17: Inhibition experiment of ARC microbial agent on peanut root rot pathogens

[0430] 5g of ARC microbial agent was mixed with a small amount of peanut root rot pathogenic fungi and inoculated into 50mL of LB liquid medium. Simultaneously, the peanut root rot pathogenic fungi were inoculated into the LB liquid medium without ARC microbial agent. After incubation in a shaker at room temperature for 3 days, the mycelium was collected, blotted dry with absorbent paper, and weighed. The inhibition rate of ARC microbial agent against the peanut root rot pathogenic fungi was calculated. The results showed that the inhibition rate of ARC microbial agent against the peanut root rot pathogenic fungi was above 91.5%.

[0431] Example 18: Use of ARC microbial agent to alleviate peanut root rot

[0432] ARC microbial agents 1-8 were mixed with peanut seeding fertilizer and applied to the field via seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Plots not treated with any of the agents served as controls, while all other plots were managed using conventional field management. After the peanuts emerged, ongoing surveys were conducted to assess the incidence of root rot in the treated and control peanuts. These survey results showed that the incidence of root rot in the peanuts treated with ARC microbial agents 1-8 was reduced by over 62.7% compared to the control group, and treatment with ARC microbial agents restored over 30% of peanut yield losses. These results demonstrate that the application of ARC microbial agents significantly reduces peanut root rot.

[0433] The above-mentioned ARC microbial agents were applied in peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar significant effects of reducing peanut root rot and its damage were achieved.

[0434] Applying the above-mentioned ARC microbial agents after peanut seedlings emerge and before the onset of root rot also achieved similar significant effects in reducing peanut root rot and its damage.

[0435] Compared with other existing schemes, the ARC microbial agent has a more prominent preventive effect on peanut root rot before the onset of the disease.

[0436] Use of ARC microbial agent in reducing bacterial diseases such as peanut wilt and their damage

[0437] Example 19:

[0438] 5g of the ARC microbial agent was mixed with a small amount of fluorescently labeled peanut bacterial wilt pathogen and inoculated into 50mL of LB liquid medium. At the same time, fluorescently labeled peanut bacterial wilt pathogen was inoculated into the LB liquid medium without the ARC microbial agent. After incubation at room temperature on a shaker for 3 days, the fluorescence intensity was measured and the inhibition rate of the ARC microbial agent against the bacterial wilt pathogen was calculated. The results showed that the inhibition rate of the ARC microbial agent against the peanut bacterial wilt pathogen was above 89%.

[0439] Uses of ARC microbial agent - Uses for alleviating peanut bacterial wilt

[0440] ARC microbial agents 1-8 were mixed with peanut seeding fertilizer and applied to the field via seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was established without any of the agents, while all other plots were managed conventionally. After the peanuts emerged, ongoing surveys were conducted to determine the incidence of bacterial wilt in the treated and control peanuts. These survey results showed that the incidence of bacterial wilt in peanuts treated with ARC microbial agents 1-8 was reduced by over 62.7% compared to the control group, and that treatment with ARC microbial agents restored soybean yield losses by over 30%. These results demonstrate that the application of ARC microbial agents significantly reduces peanut bacterial wilt, thereby significantly alleviating the seedling shortages and yield losses caused by bacterial wilt.

[0441] The above-mentioned ARC microbial agents were applied in peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar significant effects of reducing peanut wilt disease and its damage were achieved.

[0442] Applying the above-mentioned ARC microbial agents from the time peanut seedlings emerge to the time when they bloom and needle drop also achieved similar significant effects in reducing peanut bacterial wilt and its damage.

[0443] Compared with other existing schemes, the ARC microbial agent has a more prominent preventive effect on bacterial diseases such as peanut wilt before the onset of the disease.

[0444] Use of ARC microbial agent in reducing peanut root knot nematode disease and its damage

[0445] Example 20:

[0446] ARC microbial agents 1-8 were mixed with peanut seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was established without any of the agents, while all other plots were managed conventionally. After the peanuts emerged, ongoing surveys were conducted to assess the incidence of root-knot nematodes in the treated and control plots. These survey results showed that the incidence of root-knot nematodes in peanuts treated with ARC microbial agents 1-8 was reduced by over 65.2% compared to the control plot, and the ARC microbial agent treatment reversed over 30% of peanut yield losses.

[0447] The above results show that the application of ARC microbial agent has the effect of significantly reducing peanut root knot nematode disease and its damage, thereby significantly reducing the peanut yield loss caused by root knot nematode disease.

[0448] The above-mentioned ARC microbial agents were applied in peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar significant effects of reducing peanut root-knot nematode disease and its damage were achieved.

[0449] Applying the above-mentioned ARC microbial agents from the time peanut seedlings emerge to the time when they bloom and needle drop also achieved similar significant effects in reducing peanut root-knot nematode disease and its damage.

[0450] ARC microbial agent is used to reduce mildew spots and other spots on the surface of peanuts to improve marketability

[0451] Example 21: Use of ARC microbial agent - Used to reduce mold spots and other spots on the surface of peanuts to improve marketability

[0452] ARC microbial agents 1-8 were mixed with peanut seeding fertilizer and applied to the field via seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). A control plot was established without any of the agents, while all other plots were managed conventionally. At harvest, the black spotting on the surface of the peanut shells in both the treated and control groups was statistically analyzed. These results showed that peanuts treated with ARC microbial agents 1-8 showed a reduction of over 70% in black spotting on the shells. Farmers sold peanuts in the treated group for over 0.3 RMB more per pound (approximately 0.5 to 1.5 lb) than those in the control group. These results demonstrate that the application of ARC microbial agents significantly reduces surface spotting on peanut shells, improving marketability and competitiveness, and increasing production efficiency.

[0453] The above-mentioned ARC microbial agent was applied in peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar effects of significantly reducing the spots on the surface of peanut fruits and improving the commercial quality were achieved.

[0454] Applying the above-mentioned ARC microbial agent from the time peanut seedlings emerge to the time when they bloom and needle drop also achieved similar effects of significantly reducing the spots on the surface of peanut fruits and improving their marketability.

[0455] Use of ARC microbial agent to improve soybean quality and reduce the risk of aflatoxin contamination

[0456] Example 22: Use of ARC microbial agent - Use for improving soybean quality and reducing the risk of aflatoxin contamination

[0457] ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied to the field via seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Plots not treated with any of the agents served as controls, while all other plots were managed using conventional field management. After the soybeans were harvested, soybean samples were collected using national standard sampling methods. The isoflavone, protein, and amino acid content of the soybean samples were determined using standard methods to calculate the level of quality improvement. The abundance of aflatoxin-carrying Aspergillus or aflatoxin-producing fungi in soybeans was determined using a published colony count method or the PAB-01 marker molecule assay. These results showed that the application of ARC microbial agents 1-8 significantly increased the isoflavone, protein, and glutamate content of soybeans grown in the field, and reduced the abundance of aflatoxin-carrying Aspergillus or aflatoxin-producing fungi in soybeans by more than 60%, thereby reducing the risk of aflatoxin contamination. These results demonstrate that the application of ARC microbial agents significantly improved soybean quality and reduced the risk of aflatoxin contamination in soybeans grown in the field.

[0458] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar results were achieved that significantly improved soybean quality.

[0459] Similar significant effects of improving soybean quality were achieved by applying the above-mentioned ARC microbial agents from the time soybean seedlings emerge to the time when they bloom and set pods.

[0460] The use of ARC microbial agent to promote soybean nodulation and nitrogen fixation to achieve green and low-carbon production

[0461] Example 23: Use of ARC microbial agent - Use for promoting soybean nodulation and nitrogen fixation to achieve green and low-carbon production

[0462] ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was set up without any of the agents, while all others were managed using conventional field management. Continuous investigations of soybean root nodulation were conducted after soybean seedlings emerged. These findings showed that during the flowering phase, the number of root nodules in soybeans treated with agents 1-8 increased by more than 3.35 times, and the nitrogenase activity of individual soybean plants increased by more than 5.5 times. Continuous monitoring of carbon dioxide concentrations in the treated and control plots using a carbon dioxide infrared monitor revealed a reduction of more than 9.3%. These results indicate that the application of ARC microbial agents significantly promoted soybean nodulation and nitrogen fixation in the field, as well as green, low-carbon production.

[0463] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar significant effects were achieved in promoting soybean nodulation and nitrogen fixation to achieve green and low-carbon production.

[0464] Applying the above-mentioned ARC microbial agents from the time soybean seedlings emerge to the time when they bloom and set pods also achieved similar significant effects in promoting soybean nodulation and nitrogen fixation to achieve green and low-carbon production.

[0465] Application of ARC microbial agent in increasing soybean yield and total biomass

[0466] Example 24: Use of ARC microbial agent - Use for increasing soybean yield

[0467] ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). A control plot was established without any of the agents, while all other plots were managed using conventional methods. At harvest time, soybeans were harvested from one mu (approximately 1.5 acres) of fields treated with ARC microbial agents and the control plots. Calculations showed that soybean yield increases in the fields treated with ARC microbial agents 1-8 were all above 17%, achieving significant yield increases. These results demonstrate that the application of ARC microbial agents significantly improved soybean yields per unit area in large fields.

[0468] The application of the above-mentioned ARC microbial agents in soybean production by manual spreading, drone spreading, etc. during soybean sowing has also achieved similar results in significantly improving soybean yield levels.

[0469] Applying the above-mentioned ARC microbial agents from the time soybeans emerge to the time they bloom and set pods also achieved similar results in significantly increasing soybean yields.

[0470] Example 25: Use of ARC microbial agent - Use for increasing total soybean biomass

[0471] ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was established without any of the agents, while all other plots were managed conventionally. A survey was conducted at soybean maturity, and the total biomass of soybean plants treated with ARC microbial agents and the control plots was calculated. These survey results showed that the total biomass of soybean plants treated with ARC microbial agents 1-8 increased by more than 13%. These results demonstrate that the application of ARC microbial agents significantly promotes the increase in total soybean plant biomass.

[0472] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar effects of significantly increasing the total soybean biomass were achieved.

[0473] Similar significant increases in soybean biomass were achieved by applying the above-mentioned ARC microbial agents from the time soybean seedlings emerge to the time when they bloom and set pods.

[0474] Application of ARC microbial agent in soybean to prolong the nodulation and nitrogen fixation time and prevent premature aging due to nutrient deficiency

[0475] Example 26: Use of ARC microbial agent - Use to promote soybean nodulation and nitrogen fixation time to prevent premature aging due to nutrient depletion

[0476] ARC microbial agents 1-8 were mixed with soybean seeding base fertilizer and applied to the field using a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was established without any of the agents, while all other plots were managed using conventional methods. Root nodulation was continuously monitored after soybean seedlings emerged. These findings showed that soybeans treated with ARC microbial agents 1-8 developed root nodules approximately 6 days after seedling emergence, 20 days earlier than the conventional wisdom of 30 days. New nodules were still found during the grain-filling stage, with the coexistence of old, middle-aged, and young nodules, as well as infant nodules. Furthermore, measurements of soybean leaf photosynthetic rates showed a 30% increase compared to controls. Fresh, viable nodules with nitrogenase activity were still found at harvest, disproving the conventional wisdom that soybeans no longer develop new nodules after the grain-filling stage and that all nodules decay at harvest. This significantly prolonged the period of nodulation and nitrogen fixation, preventing premature aging and aging due to nutrient depletion. Ultimately, soybean yield increased by over 13.9%. These results indicate that the application of ARC microbial agents enabled earlier nodulation and a longer period of nodulation and nitrogen fixation, preventing premature aging and aging due to nutrient depletion, and contributing to higher yields.

[0477] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar significant effects were achieved in promoting soybean nodulation and nitrogen fixation, preventing premature aging due to nutrient deficiency, and increasing yield.

[0478] Applying the above-mentioned ARC microbial agents from the time soybeans emerge to the time they bloom and set pods also achieved similar significant effects in promoting soybean nodulation and nitrogen fixation, preventing premature aging due to nutrient deficiency, and increasing yield.

[0479] The use of ARC microbial agent for early nodulation, nitrogen fixation, early flowering and pod formation in soybeans

[0480] Example 27: Use of ARC microbial agent - for early nodulation and nitrogen fixation, early flowering and pod formation in soybeans

[0481] ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 100 million acre). A control plot was established without any of the agents, while all other plots were managed conventionally. After soybean seedlings emerged, continuous surveys were conducted to measure the timing of nodulation, nitrogen fixation, flowering, and pod formation in soybeans treated with ARC microbial agents and controls. These survey results showed that soybeans treated with ARC microbial agents 1-8 nodulated and fixed nitrogen more than 16 days earlier than the control, and both flowering and pod formation were at least 2 days earlier, ultimately increasing yield by over 14.7%. These results demonstrate that the application of ARC microbial agents significantly promotes earlier nodulation, nitrogen fixation, flowering, and pod formation in soybeans, contributing to higher soybean yields.

[0482] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar significant effects were achieved in promoting early nodulation and nitrogen fixation, early flowering, early pod formation, and increasing yield per unit area of ​​soybean.

[0483] Applying the above-mentioned ARC microbial agents from the time soybean seedlings emerge to the time when they bloom and set pods has also achieved similar significant effects in promoting early nodulation and nitrogen fixation, early flowering, early pod setting, and increasing yield per unit area in soybeans.

[0484] Use of ARC microbial agent to reduce soybean pod shrinkage rate and increase soybean pod plumpness

[0485] Example 28: Use of ARC microbial agent - used to reduce soybean pod shrinkage rate and increase soybean pod plumpness and 100-grain weight

[0486] ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Plots not treated with any of the agents served as controls, while all other plots were managed using conventional field management. At harvest, soybean pods were surveyed and the pod shriveling rate calculated. These survey results showed that soybeans treated with ARC microbial agents 1-8 had a pod shriveling rate reduced by over 7.3%, significantly improved pod plumpness, and increased 100-kernel weight by over 7.6%, ultimately increasing soybean yield per unit area by over 14.6%. These results demonstrate that the application of ARC microbial agents significantly reduced the pod shriveling rate, increased pod plumpness and 100-kernel weight, and ultimately boosted soybean yield per unit area.

[0487] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar effects were achieved, which significantly reduced the soybean pod shriveling rate, increased soybean pod fullness, 100-grain weight and yield.

[0488] Applying the above-mentioned ARC microbial agents from the time soybeans emerge to the time they bloom and set pods also achieved similar results in significantly reducing the rate of shrunken soybean pods, increasing soybean pod fullness, 100-grain weight, and yield.

[0489] Use of ARC microbial agent in alleviating soybean greening and its hazards

[0490] Example 29: Use of ARC microbial agent - Use for alleviating soybean greening disease and its harm

[0491] ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Plots not treated with any of the agents served as controls, while all other plots were managed using conventional field management. Each experiment was replicated three times. After soybean seedlings emerged, ongoing surveys were conducted to assess the incidence of greening in soybeans treated with ARC microbial agents and controls. These survey results showed that soybeans treated with ARC microbial agents 1-8 experienced a reduction of over 44% in greening compared to the control group, and that ARC treatments reversed soybean yield losses by over 30%.

[0492] The above results show that the application of ARC microbial agent has the effect of significantly reducing the occurrence and harm of soybean green disease.

[0493] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar significant effects of reducing soybean greening and damage were achieved.

[0494] Applying the above-mentioned ARC microbial agents from the time soybeans emerge to the time they bloom and set pods also achieved similar significant effects in reducing soybean greening and damage.

[0495] Compared with other existing solutions, the ARC microbial agent has a more prominent preventive effect on soybean green disease before it occurs. The use of ARC microbial agent to reduce fungal diseases and damage such as soybean powdery mildew and downy mildew

[0496] Uses of ARC microbial agents - Used to reduce fungal diseases and damage such as soybean powdery mildew and downy mildew

[0497] Example 30: Use of ARC microbial agent - Use for reducing soybean powdery mildew

[0498] 5g of ARC microbial agent was mixed with a small amount of soybean powdery mildew fungus and inoculated into 50mL of LB liquid medium. Simultaneously, the soybean powdery mildew fungus was inoculated into LB liquid medium without ARC. After incubation on a shaker at room temperature for 3 days, the mycelium was collected, blotted dry with absorbent paper, and weighed. The inhibition rate of ARC microbial agent against the soybean powdery mildew fungus was calculated. The results showed that the inhibition rate of ARC microbial agent against the soybean powdery mildew fungus was above 92.6%.

[0499] ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 100 million acre). A control plot was established without any of the agents, while all other plots were managed conventionally. After soybean seedlings emerged, continuous surveys were conducted to assess the incidence of powdery mildew in soybeans treated with ARC microbial agents and controls. These survey results showed that soybeans treated with ARC microbial agents 1-8 showed a reduction of more than 55% in powdery mildew compared to the control group, and that ARC treatment reversed soybean yield losses by more than 30%. These results demonstrate that the application of ARC microbial agents significantly reduces the incidence of powdery mildew in soybeans, thereby alleviating soybean production losses caused by powdery mildew.

[0500] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar significant effects of reducing soybean powdery mildew and damage were achieved.

[0501] Applying the above-mentioned ARC microbial agents after soybean seedlings emerge but before disease occurs also achieved similar significant effects in reducing soybean powdery mildew and its damage.

[0502] Compared with other existing schemes, the ARC microbial agent has a more prominent preventive effect on soybean powdery mildew before the onset of the disease.

[0503] Example 31: Use of ARC microbial agent for reducing soybean downy mildew

[0504] 5g of ARC microbial agent was mixed with a small amount of soybean downy mildew pathogen and inoculated into 50mL of LB liquid medium. Simultaneously, LB liquid medium was inoculated with soybean downy mildew pathogen without ARC microbial agent. After incubation on a shaker at room temperature for 3 days, the mycelium was collected, blotted dry with absorbent paper, and weighed. The inhibition rate of ARC microbial agent against soybean downy mildew pathogen was calculated. The results showed that the inhibition rate of ARC microbial agent against soybean downy mildew pathogen was above 93.2%.

[0505] ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). Plots not treated with any of the agents served as controls, while all other plots were managed using conventional methods. Each experiment was replicated three times. After soybean seedlings emerged, ongoing surveys were conducted to assess the incidence of downy mildew in soybeans treated with ARC microbial agents and controls. These survey results showed that soybeans treated with ARC microbial agents 1-8 had a more than 67.2% reduction in downy mildew incidence compared to the control group, and that ARC treatments reversed soybean yield losses by more than 30%. These results demonstrate that the application of ARC microbial agents significantly reduces the incidence of soybean downy mildew, thereby alleviating soybean production losses caused by downy mildew.

[0506] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar significant effects of reducing soybean downy mildew and damage were achieved.

[0507] Applying the above-mentioned ARC microbial agents after soybean seedlings emerge but before the disease occurs also achieved similar significant effects in reducing soybean downy mildew and its damage.

[0508] Compared with other existing schemes, the ARC microbial agent has a more prominent preventive effect on soybean downy mildew before the onset of the disease.

[0509] Use of ARC microbial agent to reduce soybean root rot and its damage

[0510] Example 32: Use of ARC microbial agent for reducing soybean root rot

[0511] 5g of ARC microbial agent was mixed with a small amount of soybean root rot pathogens, including Phytophthora, Pythium, Fusarium, or Rhizoctonia solani, and inoculated into 50mL of LB liquid medium. At the same time, the soybean root rot pathogens, without ARC, were also inoculated into the LB liquid medium. After incubation in a shaker at room temperature for three days, the mycelium was collected, blotted dry with absorbent paper, and weighed. The inhibition rate of ARC microbial agent against the soybean root rot pathogens was calculated. The results showed that the inhibition rate of ARC microbial agent against the soybean root rot pathogens was above 90%.

[0512] ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 100 million acres). A control plot was established without any of the agents, while all other plots were managed conventionally. After soybean seedlings emerged, ongoing surveys were conducted to assess the incidence of root rot in soybeans treated with ARC microbial agents and controls. These survey results showed that the incidence of root rot in soybeans treated with ARC microbial agents 1-8 was reduced by over 62% compared to the control group, and the ARC microbial agent treatments restored over 30% of soybean yield losses. These results demonstrate that the application of ARC microbial agents significantly reduces the incidence of soybean root rot, thereby alleviating soybean production losses caused by root rot.

[0513] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar significant effects of reducing soybean root rot were achieved.

[0514] Similar significant effects of reducing soybean root rot were achieved by applying the above-mentioned ARC microbial agents after soybean seedlings emerged but before the disease occurred.

[0515] Compared with other existing schemes, the ARC microbial agent has a more prominent preventive effect on soybean root rot before the onset of the disease.

[0516] Use of ARC microbial agent to shorten soybean pod spacing and increase pod number

[0517] Example 33 Use of ARC microbial agent - Use for shortening soybean pod spacing and increasing the number of pods

[0518] ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied to the field via seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 1.5 to 2.5 acres). A control plot was established without any of the agents, while all other plots were managed conventionally. The number of soybean pods was measured at harvest time. These results showed that the spacing between soybean pods in plants treated with agents 1-8 was, on average, 4.2 mm shorter than in the control, the number of soybean pods increased by an average of over 9.5%, and the yield per unit area increased by over 16.7%. These results demonstrate that the application of ARC microbial agents significantly shortens the spacing between soybean pods and increases the number of soybean pods, thereby significantly improving yield per unit area.

[0519] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar effects of significantly shortening the distance between soybean nodes and increasing the number of pods were achieved.

[0520] Applying the above-mentioned ARC microbial agents from the time soybean seedlings emerge to the time when they bloom and set pods also achieved similar effects of significantly shortening the spacing between soybean pods and increasing the number of pods.

[0521] Application of ARC microbial agent in increasing soybean yield in saline-alkali land

[0522] Example 34 Use of ARC microbial agent - Use for promoting soybean yield improvement in moderate to severe saline-alkali land

[0523] The pilot demonstration was conducted on moderately to severely saline-alkali soil with a pH between 8.2 and 9.2. ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied via seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu (approximately 100 million sq ft). A control plot was established without any of the agents, while all other plots were managed conventionally. Yield surveys were conducted at soybean harvest. These surveys showed that soybean yields per unit area increased by over 17.2% after the application of ARC microbial agents 1-8. These results demonstrate that the application of ARC microbial agents significantly increases soybean yields in saline-alkali soil.

[0524] The application of the above-mentioned ARC microbial agents in soybean production by manual spreading, drone spreading, etc. during soybean sowing has also achieved similar significant results in promoting the increase of soybean yield in saline-alkali land.

[0525] Similar significant effects of promoting soybean yield in saline-alkali soil were achieved by applying the above-mentioned ARC microbial agents from soybean emergence to flowering and podding.

[0526] Example 35 Use of ARC microbial agent - Use for promoting soybean yield improvement in moderately and lightly saline-alkali soil

[0527] The pilot demonstration was conducted on moderately to lightly saline-alkali soil with a pH between 7.7 and 8.7. ARC microbial agents 1-8 were mixed with soybean seeding fertilizer and applied via seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was established without any of the agents, while all other plots were managed conventionally. Yield surveys were conducted at soybean harvest. These surveys showed that soybean yields per unit area increased by over 15.5% after the application of ARC microbial agents 1-8. These results demonstrate that the application of ARC microbial agents significantly increases soybean yields in saline-alkali soil.

[0528] The application of the above-mentioned ARC microbial agents in soybean production by manual spreading, drone spreading, etc. during soybean sowing has also achieved similar significant results in promoting the increase of soybean yield in saline-alkali land.

[0529] Similar significant effects of promoting soybean yield in saline-alkali soil were achieved by applying the above-mentioned ARC microbial agents from soybean emergence to flowering and podding.

[0530] Application of ARC microbial agent - used to promote nodulation and nitrogen fixation and increase yield of leguminous vegetables such as soybeans, peas, broad beans, cowpeas, green beans, sword beans, and black-eyed peas

[0531] Example 36 Use of ARC microbial agent - Use for promoting nodulation and nitrogen fixation in cowpea and improving yield

[0532] ARC microbial agents 1-8 were mixed with cowpea seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. Plots not treated with any of the agents served as controls, while all other plots were maintained under conventional field management. Ten days after application, root nodulation and nitrogen fixation were investigated. Results showed that ARC microbial agents 1-8 increased the number of cowpea root nodules by more than three times and increased nitrogenase activity by more than 3.5 times. Fresh cowpea pods were weighed and yields were measured at each harvest. The yield per unit area of ​​fresh cowpea pods was calculated by accumulating the harvested pods. The yield increases for cowpeas treated with ARC microbial agents 1-8 were all above 12.4%, achieving significant yield increases. These results demonstrate that the application of ARC microbial agents significantly promoted cowpea nodulation and nitrogen fixation and increased cowpea yield per unit area.

[0533] The above-mentioned ARC microbial agents were applied to cowpea production by manual spreading, drone spreading, etc. during cowpea sowing, and similar significant effects of promoting nodulation and nitrogen fixation and increasing cowpea yield were achieved.

[0534] Applying the above-mentioned ARC microbial agents after cowpea seedlings emerge also achieved similar significant effects in promoting nodulation and nitrogen fixation and increasing cowpea yield.

[0535] Example 37 Use of ARC microbial agent - Use for promoting nodulation and nitrogen fixation of broad beans and improving yield

[0536] ARC microbial agents 1-8 were mixed with broad bean seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was set up without any of the agents, while all other plots were managed using conventional methods. Ten days after application, root nodulation and nitrogen fixation were investigated. Results showed that ARC microbial agents 1-8 increased root nodules by more than 3.3 times and nitrogenase activity by more than 3.6 times. When the broad beans were harvested and weighed for yield measurement, the yield increase for those treated with ARC microbial agents 1-8 was over 13.5%, achieving a significant yield increase. These results indicate that the application of ARC microbial agents significantly promoted broad bean nodulation and nitrogen fixation and increased broad bean yield per unit area.

[0537] The above-mentioned ARC microbial agent was applied to pea production by manual spreading, drone spreading, etc. during broad bean sowing, and similar significant effects of promoting nodulation and nitrogen fixation and increasing broad bean yield were achieved.

[0538] Applying the above-mentioned ARC microbial agents after the emergence of broad beans also achieved similar significant effects in promoting nodulation and nitrogen fixation and increasing broad bean yield.

[0539] Example 38 Use of ARC microbial agent - Use for promoting pea nodulation and nitrogen fixation and improving yield

[0540] ARC microbial agents 1-8 were mixed with pea seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was set up without any of the agents, while all other plots were managed using conventional methods. Ten days after application, root nodulation and nitrogen fixation were investigated. Results showed that ARC microbial agents 1-8 increased root nodules by more than 3.4 times and nitrogenase activity by more than 4 times. Peas treated with ARC microbial agents 1-8 showed yield increases exceeding 15% at harvest, significantly increasing yield. These results demonstrate that the application of ARC microbial agents significantly promoted pea nodulation and nitrogen fixation and improved field pea yields.

[0541] The above-mentioned ARC microbial agent was applied to pea production by manual spreading, drone spreading, etc. during pea sowing, and similar significant effects of promoting nodulation and nitrogen fixation and increasing pea yield were achieved.

[0542] Applying the ARC microbial agent after pea seedling emergence also achieved similar significant results in promoting nodulation and nitrogen fixation, as well as increasing pea yield. Furthermore, using the same method, applying the ARC microbial agent to other leguminous vegetables, such as soybeans, green beans, sword beans, and black-eyed peas, also achieved similar significant results in promoting nodulation and nitrogen fixation, as well as increasing yield per unit area, as seen with cowpeas, broad beans, and peas.

[0543] Use of ARC microbial agent to promote nodulation and nitrogen fixation in leguminous grain crops and increase yield

[0544] Example 39: Use of ARC microbial agent - Use for promoting nodulation and nitrogen fixation of red beans and increasing yield

[0545] ARC microbial agents 1-8 were mixed with adzuki bean seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A plot without any of the above agents was set up as a control, while all other plots were managed using conventional field management. Ten days after application of ARC microbial agents 1-8, root nodulation and nitrogen fixation were investigated. The results showed that ARC microbial agents 1-8 increased both root nodulation and nitrogenase activity by more than 2-fold. After maturity, the adzuki beans were harvested, weighed, and yields were calculated. The yield increase rates for adzuki beans treated with ARC microbial agents 1-8 were all above 9.7%, achieving a significant yield increase. These results indicate that the application of ARC microbial agents significantly promoted adzuki bean nodulation and nitrogen fixation and increased adzuki bean yields per unit area.

[0546] The above-mentioned ARC microbial agents were applied to adzuki bean production by manual spreading, drone spreading, etc. during adzuki bean sowing, and similar significant effects were achieved in promoting adzuki bean nodulation and nitrogen fixation and increasing adzuki bean yield.

[0547] Applying the above-mentioned ARC microbial agents after the emergence of red beans also achieved similar results in significantly increasing red bean yields.

[0548] Example 40: Use of ARC microbial agent - Use for promoting nodulation and nitrogen fixation of mung beans and increasing yield

[0549] ARC microbial agents 1-8 were mixed with mung bean seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was set up without any of the agents, while all other plots were managed using conventional methods. Ten days after application, root nodulation and nitrogen fixation were investigated. Results showed that ARC agents 1-8 increased the number of mung bean root nodules by more than 2.1 times and nitrogenase activity by more than 2.3 times. Mung beans were harvested and weighed after maturity, and yield per unit area was calculated. The yield increase for mung beans treated with ARC agents 1-8 was over 9.5%, achieving significant yield increases. These results demonstrate that the application of ARC microbial agents significantly promoted green nodulation and nitrogen fixation and increased mung bean yield per unit area.

[0550] The above-mentioned ARC microbial agents were applied in mung bean production by manual spreading, drone spreading, etc. during mung bean sowing, and similar significant effects of promoting nodulation and nitrogen fixation and increasing mung bean yield were achieved.

[0551] Applying the above-mentioned ARC microbial agents after mung bean seedlings emerged also achieved similar significant effects in promoting nodulation and nitrogen fixation and increasing mung bean yield.

[0552] Example 41: Use of ARC microbial agent - Use for promoting nodulation and nitrogen fixation of peas and increasing yield

[0553] ARC microbial agents 1-8 were mixed with pea seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A control plot was set up without any of the agents, while all other plots were managed using conventional field management. Ten days after application, root nodulation and nitrogen fixation were investigated. Results showed that ARC agents 1-8 increased the number of pea root nodules by more than 2-fold and nitrogenase activity by more than 2.1-fold. After maturity, the peas were harvested, weighed, and yields calculated. The yield increases for peas treated with ARC agents 1-8 were all above 7.3%, achieving significant yield increases. These results indicate that the application of ARC microbial agents significantly promoted green nodulation and nitrogen fixation and increased pea yields per unit area.

[0554] The above-mentioned ARC microbial agents were applied to the production of mung beans by manual spreading, drone spreading, etc. during the sowing of mung beans, and similar significant effects of promoting nodulation and nitrogen fixation and increasing mung bean yield were achieved.

[0555] The application of the above-mentioned ARC microbial agents after the emergence of the mung bean seedlings also achieved similar significant effects in promoting nodulation and nitrogen fixation and increasing the yield of the mung bean.

[0556] Example 42: Use of ARC microbial agent - Use for promoting nodulation and nitrogen fixation of lentil and increasing yield

[0557] ARC microbial agents 1-8 were mixed with lentil seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. A plot without any of the agents was set up as a control, while all other plots were managed using conventional field management. Ten days after application, root nodulation and nitrogen fixation were investigated. Results showed that ARC microbial agents 1-8 increased root nodules by more than 2.3 times and nitrogenase activity by more than 2.8 times. After maturity, the lentils were harvested, weighed, and yields were calculated. The yield increase for lentil treated with ARC microbial agents 1-8 was over 10.9%, achieving a significant yield increase. These results indicate that the application of ARC microbial agents significantly promoted green nodulation and nitrogen fixation and increased lentil yield per unit area.

[0558] The above-mentioned ARC microbial agents were applied to lentil production by manual spreading, drone spreading, etc. during lentil sowing, and similar significant effects of promoting nodulation and nitrogen fixation and increasing lentil yield were achieved.

[0559] Applying the above-mentioned ARC microbial agents after the emergence of lentil seedlings also achieved similar significant effects in promoting nodulation and nitrogen fixation and increasing lentil yield.

[0560] Use of ARC microbial agent in promoting nodulation and nitrogen fixation of leguminous forage grasses and increasing yield

[0561] Example 43: Use of ARC microbial agent - Use for promoting nodulation and nitrogen fixation and increasing the yield of leguminous forage alfalfa

[0562] ARC microbial agents 1-8 were mixed with alfalfa seeding fertilizer and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. Plots not treated with any of the agents served as controls, while all other plots were managed under conventional conditions. Ten days after application, root nodulation and nitrogen fixation were investigated. Results showed that ARC agents 1-8 increased root nodules by more than 2.6 times and nitrogenase activity by more than 2.9 times in Astragalus adductus. After each alfalfa harvest, yield was measured by weighing and calculating the annual per-mu yield of all alfalfa harvested within a year. The yield increase for alfalfa treated with ARC agents 1-8 was over 10%, demonstrating significant yield increases. These results demonstrate that the application of ARC microbial agents significantly promotes nodulation and nitrogen fixation and increases alfalfa yield in the field.

[0563] The above-mentioned ARC microbial agents were applied to alfalfa production by manual spreading, drone spreading, etc. during alfalfa sowing, and similar significant effects of promoting nodulation and nitrogen fixation and increasing alfalfa yield were achieved.

[0564] Applying the above-mentioned ARC microbial agent after alfalfa seedlings emerge or after the previous crop is harvested also achieved similar significant effects in promoting nodulation and nitrogen fixation and increasing alfalfa yield.

[0565] Example 44: Use of ARC microbial agent - Use for promoting nodulation and nitrogen fixation and increasing the yield of leguminous forage grass Astragalus membranaceus

[0566] ARC microbial agents 1-8 were mixed with the seeding base fertilizer for the legume forage grass Astragalus membranaceus and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. Plots not treated with any of the agents served as controls, while all other plots were managed under conventional conditions. Ten days after application, root nodulation and nitrogen fixation were investigated. Results showed that ARC agents 1-8 increased root nodules by more than 2.7 times and nitrogenase activity by more than 3 times. Yields of Astragalus membranaceus were measured after each harvest. The annual yield per mu of Astragalus membranaceus harvested within a year was calculated. The yield increase for Astragalus membranaceus treated with ARC agents 1-8 was over 13%, achieving significant yield increases. These results demonstrate that the application of ARC microbial agents significantly promotes nodulation and nitrogen fixation and increases the yield of Astragalus membranaceus in the field.

[0567] The above-mentioned ARC microbial agent was applied to the production of the legume forage grass Astragalus membranaceus by manual spreading, drone spreading, drip irrigation, etc. during sowing, and similar significant effects of promoting nodulation and nitrogen fixation and increasing Astragalus membranaceus yield were achieved.

[0568] Applying the above-mentioned ARC microbial agent after the emergence of the leguminous forage grass Astragalus membranaceus or after the previous crop was harvested also achieved similar significant effects in promoting nodulation and nitrogen fixation and increasing Astragalus membranaceus yield.

[0569] Example 45: Use of ARC microbial agent - Use for promoting nodulation and nitrogen fixation and increasing the yield of leguminous forage grass Sainfoin

[0570] ARC microbial agents 1-8 were mixed with a seeding base fertilizer for the legume forage grass, Sainfoin, and applied to the field via a seed drill at a cumulative rate of 80 to 100 billion viable bacteria per mu. Plots not treated with any of the agents served as controls, while all other plots were managed under conventional conditions. Ten days after application, root nodulation and nitrogen fixation were investigated. Results showed that ARC agents 1-8 increased root nodules by more than 2.5 times and nitrogenase activity by more than 2.7 times. Yields were measured after each harvest, and the annual yield per mu of Sainfoin was calculated based on the cumulative yield of all Sainfoin harvests within a year. The yield increases for Sainfoin treated with ARC agents 1-8 were all above 11%, demonstrating significant increases. These results demonstrate that the application of ARC agents significantly promoted nodulation and nitrogen fixation and increased the yield of Sainfoin in the field.

[0571] The above-mentioned ARC microbial agent was applied to the production of the legume forage grass sainfoin by manual spreading, drone spreading, drip irrigation, etc. during sowing, and similar significant effects of promoting nodulation and nitrogen fixation and increasing sainfoin yield were achieved.

[0572] Applying the above-mentioned ARC microbial agent after the emergence of the leguminous forage grass Sainfoin or after the previous crop was harvested also achieved similar significant effects in promoting nodulation and nitrogen fixation and increasing the yield of Sainfoin.

[0573] In addition, the application of ARC microbial agents 1 to 8 in the production of other legume forages such as clover and astragalus has also achieved similar effects in promoting nodulation and nitrogen fixation and increasing yield levels as in the production of alfalfa, Astragalus and Sainfoin.

[0574] The use of ARC microbial agents to promote carbon emission reduction and improve arable land quality in the production of leguminous crops such as soybeans and peanuts

[0575] Example 46: Use of ARC microbial agent - Use to promote carbon emission reduction in soybean production and improve arable land quality

[0576] The above ARC microbial agents 1 to 8 were respectively mixed with soybean seeding base fertilizer and applied into the field through a seeder.

[0577] The application amount of the above-mentioned ARC microbial agent is: 80 billion to 100 billion live bacteria per mu.

[0578] The above-mentioned soybean sowing base fertilizer dosage: nitrogen fertilizer is reduced by 20%, 30%, and 40% compared with the conventional dosage, and other element fertilizers are all used in conventional dosages.

[0579] At the same time, a plot without application of any of the above-mentioned microbial agents and without reduction of nitrogen fertilizer was set up as a control, and the others were managed under conventional field management.

[0580] Post-harvest soybean yield measurements revealed that fields treated with ARC Microbial Agents 1-8 at a 20% nitrogen reduction rate saw yield increases of over 15% per mu, fields treated with ARC Microbial Agents at a 30% nitrogen reduction rate saw yield increases of over 9%, and fields treated with ARC Microbial Agents at a 40% nitrogen reduction rate saw yield increases of over 6.3%. It's generally accepted in the industry that for every kilogram of nitrogen fertilizer, such as urea, produced by a factory, approximately 10 kilograms of carbon dioxide are emitted. Therefore, reducing nitrogen fertilizer use with ARC Microbial Agents can significantly reduce carbon emissions from soybean production.

[0581] Metagenomic analysis revealed that in soybean soil samples treated with ARC microbial agents 1-8 at 20%, 30%, and 40% nitrogen fertilizer reductions, the abundance of pests such as Aspergillus flavus, Fusarium sclerotiorum, Rhizoctonia solani, and Ralstonia solanacearum decreased significantly, with reductions of at least 33% across all treatments. Furthermore, the abundance of microorganisms beneficial to soil and plants, such as Rhizobia, Bradyrhizobia, and Bacillus, increased significantly, with increases of at least 20% across all treatments. Furthermore, organic matter content in the topsoil increased by at least 6.7% across all ARC microbial agent treatments, significantly improving soil porosity—strengthening soil structure, water retention, and air permeability. In summary, the application of ARC microbial agents to reduce nitrogen fertilizer use in soybeans significantly improved farmland quality.

[0582] The above results show that the application of ARC microbial agent has the effect of significantly promoting carbon emission reduction in soybean production and improving the quality of cultivated land.

[0583] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar significant effects of promoting carbon emission reduction in soybean production and protecting arable land were achieved.

[0584] Applying the above-mentioned ARC microbial agents from soybean emergence to flowering and podding stage also achieved similar significant effects in promoting carbon emission reduction in soybean production and protecting arable land.

[0585] Example 47: Use of ARC microbial agent - Use to promote carbon emission reduction in peanut production and improve arable land quality

[0586] The above ARC microbial agents 1 to 8 were mixed evenly with peanut sowing base fertilizer respectively, and applied into the field through a seeder.

[0587] The application amount of the above-mentioned ARC microbial agent is: 80 billion to 100 billion live bacteria per mu.

[0588] The above-mentioned peanut sowing base fertilizer dosage: nitrogen fertilizer is reduced by 20%, 30%, and 40% compared with the conventional dosage, and other element fertilizers are all in conventional dosage.

[0589] At the same time, a plot without application of any of the above-mentioned microbial agents and without reduction of nitrogen fertilizer was set up as a control, and the others were managed under conventional field management.

[0590] Post-harvest peanut yield measurements revealed that fields treated with ARC Microbial Agents 1-8 and a 20% nitrogen reduction increased yields by over 18.3% per mu, fields treated with ARC Microbial Agents at a 30% nitrogen reduction increased yields by over 10.5%, and fields treated with ARC Microbial Agents at a 40% nitrogen reduction increased yields by over 6.6%. It's generally accepted in the industry that for every kilogram of nitrogen fertilizer, such as urea, produced by a factory, approximately 10 kilograms of carbon dioxide are emitted. Therefore, reducing nitrogen fertilizer use with ARC Microbial Agents can significantly reduce carbon emissions from peanut production.

[0591] Metagenomic analysis revealed that in topsoil samples from peanut fields treated with ARC microbial agents 1-8 at 20%, 30%, and 40% nitrogen fertilizer reductions, the abundance of pests such as Aspergillus flavus, Fusarium sclerotiorum, Rhizoctonia solani, and Ralstonia solanacearum decreased significantly, with reductions of at least 33.6% across all treatments. Furthermore, the abundance of microorganisms beneficial to soil and plants, such as Rhizobia, Bradyrhizobia, and Bacillus, increased significantly, with increases of at least 22.5% across all treatments. Furthermore, topsoil organic matter content increased by at least 7.3% across all ARC microbial agent treatments, and soil porosity improved significantly, with enhanced properties such as structure, water retention, and air permeability. In summary, the application of ARC microbial agents to reduce nitrogen fertilizer use in peanuts significantly improved farmland quality.

[0592] The above results show that the application of ARC microbial agent has the effect of significantly promoting carbon emission reduction in peanut production and improving the quality of cultivated land.

[0593] The above-mentioned ARC microbial agents were applied to peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar significant effects of promoting carbon emission reduction in peanut production and protecting arable land were achieved.

[0594] Applying the above-mentioned ARC microbial agents from the time peanuts emerge to the flowering and podding stage has also achieved similar significant effects in promoting carbon emission reduction in peanut production and protecting arable land.

[0595] Example 48: Use of ARC microbial agent - Use for promoting carbon emission reduction in broad bean production and improving arable land quality

[0596] The above ARC microbial agents 1 to 8 were respectively mixed with broad bean sowing base fertilizer and applied into the field through a seeder.

[0597] The application amount of the above-mentioned ARC microbial agent is: 80 billion to 100 billion live bacteria per mu.

[0598] The above-mentioned dosage of base fertilizer for broad bean sowing: nitrogen fertilizer is reduced by 20%, 30%, and 40% compared with the conventional dosage, and other element fertilizers are all in conventional dosage.

[0599] At the same time, a plot without application of any of the above-mentioned microbial agents and without reduction of nitrogen fertilizer was set up as a control, and the others were managed under conventional field management.

[0600] After harvesting the broad beans, yield measurements showed that fields treated with ARC Microbial Agents 1-8 and a 20% nitrogen reduction increased yields by over 13.6% per mu, fields treated with ARC Microbial Agents at a 30% nitrogen reduction increased yields by over 9.2%, and fields treated with ARC Microbial Agents at a 40% nitrogen reduction increased yields by over 6.3%. It's generally accepted in the industry that for every kilogram of nitrogen fertilizer, such as urea, produced by a factory, approximately 10 kilograms of carbon dioxide are emitted. In summary, reducing nitrogen fertilizer use with ARC Microbial Agents can significantly reduce carbon emissions from broad bean production.

[0601] Metagenomic analysis revealed that in topsoil samples treated with ARC microbial agents 1-8 at 20%, 30%, and 40% nitrogen fertilizer reduction rates, the abundance of pests such as Aspergillus flavus, Fusarium sclerotiorum, Rhizoctonia solani, and Ralstonia solanacearum decreased significantly, with reductions of at least 29% across all treatments. Furthermore, the abundance of microorganisms beneficial to soil and plants, such as Rhizobia, Bradyrhizobia, and Bacillus, increased significantly, with increases of at least 23% across all treatments. Furthermore, topsoil organic matter content increased by at least 7.9% across all ARC microbial agent treatments, and soil porosity improved significantly, with enhanced structure, water retention, and air permeability. In summary, the application of ARC microbial agents to reduce nitrogen fertilizer use significantly improved arable land quality.

[0602] The above results show that the application of ARC microbial agent has the effect of significantly promoting carbon emission reduction in broad bean production and improving the quality of cultivated land.

[0603] The above-mentioned ARC microbial agents were applied to broad bean production by manual spreading, drone spreading, etc. during broad bean sowing, and similar significant effects of promoting carbon emission reduction in broad bean production and protecting arable land were achieved.

[0604] Applying the above-mentioned ARC microbial agents from the time broad beans emerge to the time they bloom and set pods has also achieved similar significant effects in promoting carbon emission reduction in broad bean production and protecting arable land.

[0605] In addition, the application of the above-mentioned ARC microbial agent test program on other legumes such as red beans, mung beans, peas, cowpeas, alfalfa, and astragalus has achieved similar effects as its application on soybeans, peanuts, and broad beans - promoting carbon emission reduction in legume production and improving the quality of arable land.

[0606] The use of ARC microbial agent to reduce the harm of continuous cropping of leguminous crops such as peanuts and soybeans

[0607] Example 49: Use of ARC microbial agent - Use for reducing the harm of continuous cropping in soybean production

[0608] A soybean field with many years of continuous planting was selected to conduct a demonstration of resistance to repeated cropping. The above-mentioned ARC microbial agents 1 to 8 were mixed with soybean seeding base fertilizer and applied to the field through a seed drill. The application rate of the agents was 80 billion to 100 billion viable bacteria per mu. At the same time, a plot without any of the above-mentioned agents was set up as a control. The other plots were managed with conventional field management. After soybean sowing, irregular and continuous surveys were carried out. The survey results showed that compared with the control group, the soybeans treated with agents 1 to 8 had: (1) stronger seedlings; (2) the number of diseased plants in the treated group was significantly reduced, and the disease rate in the treated group was reduced by more than 75% compared with the control group; (3) the final yield of the treated group was more than 30% higher than that of the control group. The above results show that the application of ARC microbial agents has a significant effect in reducing the harm of repeated cropping in soybean production.

[0609] The above-mentioned ARC microbial agents were applied in soybean production by manual spreading, drone spreading, etc. during soybean sowing, and similar significant effects of reducing the damage caused by continuous cropping in soybean production were achieved.

[0610] Applying the above-mentioned ARC microbial agents from the time soybeans emerge to the time they bloom and set pods also achieved similar significant results in reducing the damage caused by repeated cropping in soybean production.

[0611] Example 50: Use of ARC microbial agent - Use for reducing the harm of continuous cropping in peanut production

[0612] A field with peanuts planted continuously for many years was selected to conduct a trial demonstration on resistance to repeated cropping. The above-mentioned ARC microbial agents 1 to 8 were mixed with peanut seeding base fertilizer and applied to the field through a seed drill. The application rate of the agents was 80 billion to 100 billion viable bacteria per mu. At the same time, a plot without any of the above-mentioned agents was set up as a control. The other plots were managed with conventional field management. After the peanuts were sown, irregular and continuous surveys were conducted. The survey results showed that compared with the control group, the peanuts treated with agents 1 to 8 had: (1) stronger seedlings; (2) the number of diseased plants in the treated group was significantly reduced, and the diseased plant rate in the treated group was reduced by more than 71% compared with the control group; (3) the final yield of the treated group was more than 22% higher than that of the control group. The above results show that the application of ARC microbial agents has a significant effect in reducing the harm of repeated cropping in peanut production.

[0613] The above-mentioned ARC microbial agents were applied to peanut production by manual spreading, drone spreading, etc. during peanut sowing, and similar significant effects of reducing the damage caused by repeated cropping in peanut production were achieved.

[0614] Applying the above-mentioned ARC microbial agents from the time peanuts emerge to the flowering and podding stage also achieved similar significant effects in reducing the damage caused by repeated cropping in peanut production.

[0615] In addition, the above-mentioned ARC microbial agent resistance to continuous cropping obstacle test demonstration program was applied to legumes such as broad beans, peas, cowpeas, green beans, and alfalfa, and achieved similar effects as soybeans and peanuts.

[0616] The use of ARC microbial agent in corn production to control bacteria and reduce toxicity, improve quality and safety, and reduce losses

[0617] Example 51: Use of ARC microbial agent - Use in corn production to control bacteria and reduce toxicity, improve quality and safety, and reduce losses

[0618] The above-mentioned ARC microbial agents 1 to 8 were mixed with corn seeding base fertilizer and applied to the field through a seed drill. The application rate of the microbial agents was 80 billion to 100 billion viable bacteria per mu. At the same time, a plot without any of the above microbial agents was set up as a control. The other plots were managed under conventional field management. After the above-mentioned corn was harvested, representative corn samples were collected. (1) The abundance of toxin-producing fungi carrying aflatoxin and fusarium toxin in these samples was determined by the classic colony counting method. The reduction rate of the abundance of toxin-producing fungi carried by corn was calculated, which is the control effect of toxin-producing fungi; (2) After the samples were placed under the same conditions for 6 months, the contamination levels of aflatoxin and fusarium toxin were determined by the national standard method. The control effect of aflatoxin and fusarium toxin was calculated. These test results show that agents 1-8 achieved over 53% effectiveness against aflatoxins and Fusarium toxin-producing fungi in field corn kernels, over 80% effectiveness against aflatoxins in corn, and over 60% effectiveness against Fusarium toxins in corn, significantly reducing grain losses due to excessive toxin contamination. These results demonstrate that the application of ARC microbial agents in corn production has significant potential for controlling bacteria and toxicity, improving corn quality and safety, and reducing losses.

[0619] The above-mentioned ARC microbial agents were applied in corn production by manual spreading, drone spreading, etc. during corn sowing, and similar effects of controlling bacteria and reducing toxicity, improving corn quality and safety, and reducing losses were achieved.

[0620] Applying the above-mentioned ARC microbial agents from the time corn seedlings emerge to the silking stage has also achieved similar effects in controlling bacteria and reducing toxicity, improving corn quality and safety, and reducing losses.

[0621] ARC microbial agent is used in wheat production to control bacteria and reduce toxicity, improve quality and safety levels and reduce losses.

[0622] Example 52 Use of ARC Microbial Agent - Use in Wheat Production to Control Bacteria and Reduce Toxicity and Improve Quality and Safety

[0623] The above-mentioned ARC microbial agents 1 to 8 were mixed with wheat seeding base fertilizer and applied to the field by a seed drill. The application rate of the microbial agents was 80 billion to 100 billion viable bacteria per mu. At the same time, a plot without any of the above microbial agents was set up as a control, and the others were managed in a conventional field. After the wheat was harvested, representative wheat samples were collected. (1) The abundance of Fusarium carried by these samples was determined by the classic colony counting method, and the reduction rate of Fusarium abundance in wheat was calculated, that is, the control effect on toxin-producing fungi; (2) The contamination level of Fusarium toxins such as vomitoxin and zearalenone was determined by the national standard method, and the control effect on Fusarium toxins was calculated. These test results showed that the control effect of microbial agents 1 to 8 on Fusarium carried by wheat grains in the field was more than 55%, and the control effect on Fusarium mycotoxins in wheat was more than 65%, significantly reducing the wheat losses caused by excessive mycotoxin contamination. The above results show that the application of ARC microbial agent in wheat production has significant effects on controlling bacteria and reducing toxicity, improving wheat quality and safety, and reducing losses.

[0624] The above-mentioned ARC microbial agents were applied in wheat production by manual spreading, drone spreading, etc. during wheat sowing, and similar effects of controlling bacteria and reducing toxicity, improving wheat quality and safety, and reducing losses were achieved.

[0625] Applying the above-mentioned ARC microbial agents from the time wheat seedlings emerge to the time it heads and flowers has also achieved similar effects in controlling bacteria and reducing toxicity, improving wheat quality and safety, and reducing losses.

[0626] The use of ARC microbial agent in the production of ARC + macronutrient compound fertilizer and its application in leguminous crops such as soybeans and peanuts

[0627] Example 53: Use of ARC microbial agent - for producing ARC + macronutrient compound fertilizer

[0628] The ARC microbial agents 1-8 are mixed at a dosage of no less than 80 billion viable bacteria per mu (approximately 100 million acres) with the conventional dosage of macronutrient fertilizers per mu (approximately 100 million acres). The ARC microbial agents are then physically adsorbed and fixed onto the macronutrient fertilizers to create eight ARC+macronutrient compound fertilizers 1-8. The macronutrient fertilizers can be purchased directly from the market or prepared using nitrogen fertilizers, phosphate fertilizers, potash fertilizers, and the like as raw materials according to common mixing ratios.

[0629] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the macronutrient fertilizer particles for adsorption.

[0630] Before the above-mentioned ARC microbial agent spraying and adsorption operation, the adsorption microbial protective agent can be sprayed on the surface of the macro-element fertilizer particles.

[0631] The above-mentioned microbial protective agent can be inorganic carbon, sulfur or biological organic matter and other materials, which can be purchased from the market.

[0632] Example 54: Application of ARC+macronutrient compound fertilizer on leguminous crops such as soybeans and peanuts

[0633] The eight ARC+macronutrient compound fertilizers 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with equal amounts of the same macronutrient compound fertilizers served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the macronutrient compound fertilizers in the control fields performed their intended function. Compared to the control fields, the eight ARC+macronutrient compound fertilizers 1-8 significantly promoted nodulation and nitrogen fixation, with nodule number increasing by more than 3.2 times, nitrogenase activity per plant increasing by more than 9 times, and yield per unit area increasing by more than 14%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial inoculants described in the above examples.

[0634] The above results show that ARC microbial agent can be used to produce ARC+macro-element compound fertilizer, and ARC+macro-element compound fertilizer can have the effects of both macro-element fertilizer and ARC microbial agent.

[0635] The use of ARC microbial agent in the production of ARC + medium and trace element compound fertilizer and its application in leguminous crops such as soybeans and peanuts

[0636] Example 55: Use of ARC microbial agent - for the production of ARC + medium and trace element compound fertilizer

[0637] The ARC microbial agents 1-8 are mixed at a dosage of no less than 80 billion viable bacteria per mu (approximately 80 billion active bacteria counts) with a medium- and trace-element compound fertilizer (approximately 80 billion active bacteria counts) per mu (approximately 80 billion active bacteria counts) and the dosage of the ARC microbial agents 1-8. The ARC microbial agents are then physically adsorbed and fixed onto the medium- and trace-element fertilizers to create eight ARC+medium- and trace-element compound fertilizers 1-8. The medium- and trace-element fertilizers can be purchased directly from the market, or prepared using magnesium fertilizers, sulfur fertilizers, calcium fertilizers, iron fertilizers, boron fertilizers, zinc fertilizers, silicon fertilizers, manganese fertilizers, copper fertilizers, molybdenum fertilizers, etc., as raw materials and then mixed according to common mixing ratios.

[0638] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the medium and trace element fertilizer particles for adsorption.

[0639] Before the above-mentioned ARC microbial agent spraying and adsorption operation, the adsorption microbial protective agent can be sprayed on the surface of the medium and trace element fertilizer particles.

[0640] The above-mentioned microbial protective agent can be inorganic carbon, sulfur or biological organic matter and other materials, which can be purchased from the market.

[0641] Example 56: Application of ARC+ medium and trace element compound fertilizer on leguminous crops such as soybeans and peanuts

[0642] The eight ARC+micronutrient compound fertilizers 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans. Fields treated with the same amount of micronutrient compound fertilizers served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the micronutrient fertilizers in the control fields performed their normal functions. Compared to the control fields, the eight ARC+micronutrient compound fertilizers 1-8 significantly promoted nodulation and nitrogen fixation, increasing nodule number by more than 3.3 times, increasing nitrogenase activity per plant by more than 10.2 times, and increasing yield per unit area by more than 15.3%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial inoculants described in the above examples.

[0643] The above results indicate that ARC microbial agent can be used to produce ARC+medium and trace element compound fertilizer, which has the effects of medium and trace element fertilizer and ARC microbial agent.

[0644] The use of ARC microbial agent in the production of ARC+ compound fertilizer / compound fertilizer and its application in leguminous crops such as soybeans and peanuts

[0645] Example 57: Use of ARC microbial agent - Production of ARC+ compound fertilizer and its application on leguminous crops such as soybeans and peanuts

[0646] The ARC microbial agents 1-8 are mixed with the conventional compound fertilizer dosage per mu (at a rate of no less than 80 billion viable bacteria per mu) and then physically adsorbed and fixed onto the compound fertilizer to create eight ARC+ compound fertilizers 1-8. These compound fertilizers can be purchased directly from the market or prepared using nitrogen fertilizers, phosphate fertilizers, potash fertilizers, and other raw materials, then mixed according to common mixing ratios.

[0647] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the compound fertilizer particles for adsorption.

[0648] Before the above-mentioned ARC microbial agent spraying and adsorption operation, a microbial protective agent can be sprayed and adsorbed on the surface of the compound fertilizer particles.

[0649] The above-mentioned microbial protective agent can be inorganic carbon, sulfur or biological organic matter and other materials, which can be purchased from the market.

[0650] The eight ARC+compound fertilizers 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same amount of compound fertilizers served as controls, and ongoing surveys were conducted after sowing. These surveys showed that the compound fertilizers in the control fields performed their intended function. Compared to the control fields, the eight ARC+compound fertilizers 1-8 significantly promoted nodulation and nitrogen fixation, with nodule numbers increasing by more than 3.2 times, nitrogenase activity per plant increasing by more than 9 times, and yield per unit area increasing by more than 14%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the above examples.

[0651] The above results indicate that ARC microbial agent can be used to prepare ARC+ compound fertilizer, and it has the effects of both compound fertilizer and ARC microbial agent.

[0652] Example 58: Use of ARC microbial agent - production of ARC+ compound fertilizer and application on leguminous crops such as soybeans and peanuts

[0653] The ARC microbial agents 1-8 are mixed with the conventional amount of compound fertilizer per mu (approximately 80 billion active bacteria per mu) and then physically adsorbed onto the compound fertilizer to create eight ARC+compound fertilizers 1-8. These compound fertilizers can be purchased directly from the market.

[0654] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the compound fertilizer particles for adsorption.

[0655] Before the above-mentioned ARC microbial agent spraying and adsorption operation, a microbial protective agent can be sprayed and adsorbed on the surface of the compound fertilizer particles.

[0656] The above-mentioned microbial protective agent can be inorganic carbon, sulfur or biological organic matter and other materials, which can be purchased from the market.

[0657] The eight ARC+compound fertilizers 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans. Fields treated with the same amount of compound fertilizers served as controls, and ongoing surveys were conducted after sowing. These surveys showed that the compound fertilizers in the control fields performed their intended function. Compared to the control fields, the eight ARC+compound fertilizers 1-8 significantly promoted nodulation and nitrogen fixation, with nodule numbers increasing by more than 2.95 times, nitrogenase activity per plant increasing by more than 9.97 times, and yield per unit area increasing by more than 14.76%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the above examples.

[0658] The use of ARC microbial agent in the production of ARC+ organic fertilizer and its application in leguminous crops such as soybeans and peanuts

[0659] Example 59: Use of ARC microbial agent - for producing ARC+ organic fertilizer

[0660] The ARC microbial agents 1-8 are mixed with the conventional amount of organic fertilizer per mu of land at a dosage of no less than 80 billion viable bacteria per mu of land. The ARC microbial agents are then physically adsorbed and fixed onto the organic fertilizer to create eight types of ARC + organic fertilizers 1-8. The organic fertilizers can be purchased directly from the market.

[0661] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the organic fertilizer particles for adsorption.

[0662] Before the above-mentioned ARC microbial agent spraying and adsorption operation, a microbial protective agent can be sprayed and adsorbed on the surface of the organic fertilizer particles.

[0663] The above-mentioned microbial protective agent can be inorganic carbon, sulfur or biological organic matter and other materials, which can be purchased from the market.

[0664] Example 60: Application of ARC+ organic fertilizer on leguminous crops such as soybeans and peanuts

[0665] The eight ARC+ organic fertilizers (1-8) were used as seed fertilizers in leguminous crops such as peanuts and soybeans. Fields treated with the same amount of organic fertilizers served as controls, and ongoing surveys were conducted after sowing. These surveys showed that the organic fertilizers in the control fields performed their intended function. Compared to the control fields, the eight ARC+ organic fertilizers (1-8) significantly promoted nodulation and nitrogen fixation, increasing nodule number by more than 3.7 times, nitrogenase activity per plant by more than 12.5 times, and yield per unit area by more than 16.2%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the above examples.

[0666] The above results show that ARC microbial agent can be used to produce ARC+ organic fertilizer, and ARC+ organic fertilizer can have the effects of both organic fertilizer and ARC microbial agent.

[0667] The use of ARC microbial agent in the production of ARC+ microbial agent and its application in leguminous crops such as soybeans and peanuts

[0668] Example 61: Use of ARC microbial agent - Use for producing ARC+ microbial agent

[0669] The above-mentioned ARC microbial agents 1 to 8 are mixed in proportion with the amount of microbial agents per mu of land at a dosage of not less than 80 billion viable bacteria per mu of land and ARC microbial agents 1 to 8 respectively, the bacterial liquid / powder of the microbial agent and the bacterial liquid / powder of the ARC microbial agent are uniformly mixed by conventional physical methods, and then adsorbed onto a carrier by physical methods to prepare ARC+ microbial agent.

[0670] The above-mentioned microbial agent is Bacillus subtilis, which can be directly purchased from the market, or can be obtained by isolating, identifying, screening and fermenting from nodules of leguminous crops through conventional methods.

[0671] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the carrier particles for adsorption.

[0672] The carrier can be a mixture of carbon powder and humus at a ratio of 9:1, or a mixture of one or two or more of carbon powder, carbon powder, humus, zeolite powder, diatomaceous earth, vermiculite, bentonite, weathered coal, etc.

[0673] The eight ARC+ microbial agents 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same microbial agents served as controls, and ongoing surveys were conducted after sowing. These surveys showed that the eight ARC+ microbial agents 1-8 significantly promoted nodulation and nitrogen fixation, increasing nodule number by more than 4.5 times, nitrogenase activity per plant by more than 13.5 times, and yield per unit area by more than 15.5%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the examples above.

[0674] The above results indicate that ARC microbial agent can be used to prepare ARC+microbial agent, and has the effects of both microbial agent and ARC microbial agent.

[0675] The use of ARC microbial agent in the production of ARC+ blended fertilizer and its application in leguminous crops such as soybeans and peanuts

[0676] Example 62: Use of ARC microbial agent - for producing ARC+ blended fertilizer

[0677] The ARC microbial agents 1-8 are mixed with the conventional fertilizer blend at a dosage of no less than 80 billion viable bacteria per mu (approximately 100 million acres) and then physically adsorbed onto the blended fertilizer to create eight ARC+ blended fertilizers 1-8. These blended fertilizers can be purchased directly from the market or prepared using nitrogen fertilizers, phosphate fertilizers, potash fertilizers, and other raw materials in commonly used proportions.

[0678] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the mixed fertilizer particles for adsorption.

[0679] Before the above-mentioned ARC microbial agent spraying and adsorption operation, the adsorption microbial protective agent can be sprayed on the surface of the mixed fertilizer particles.

[0680] The above-mentioned microbial protective agent can be inorganic carbon, sulfur or biological organic matter and other materials, which can be purchased from the market.

[0681] Example 63: Application of ARC+ blended fertilizer on leguminous crops such as soybeans and peanuts

[0682] The eight ARC+ fertilizer blends 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same blends served as controls, and ongoing surveys were conducted after sowing. These surveys showed that the fertilizer blends in the control fields performed their intended function. Compared to the control fields, the eight ARC+ fertilizer blends 1-8 significantly promoted nodulation and nitrogen fixation, with nodule numbers increasing by more than 2.77 times, nitrogenase activity per plant increasing by more than 8.95 times, and yield per unit area increasing by more than 13.9%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the above examples.

[0683] The above results indicate that ARC microbial agent can be used to prepare ARC+ blended fertilizer, and it has the effects of both blended fertilizer and ARC microbial agent.

[0684] The use of ARC microbial agent in the production of ARC+ slow-release fertilizer and its application in leguminous crops such as soybeans and peanuts

[0685] Example 64: Use of ARC microbial agent - for producing ARC+ slow-release fertilizer

[0686] The ARC microbial agents 1-8 are mixed at a dosage of no less than 80 billion viable bacteria per mu (approximately 100 million acres) with the slow-release fertilizers per mu (approximately 100 million acres). The ARC microbial agents are then physically adsorbed and fixed onto the slow-release fertilizers to create eight types of ARC + slow-release fertilizers 1-8. These slow-release fertilizers can be purchased directly from the market.

[0687] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the slow-release fertilizer particles for adsorption.

[0688] Before the above-mentioned ARC microbial agent spraying and adsorption operation, the adsorbed microbial protective agent can be sprayed on the surface of the slow-release fertilizer particles.

[0689] The above-mentioned microbial protective agent can be inorganic carbon, sulfur or biological organic matter and other materials, which can be purchased from the market.

[0690] Example 65: Application of ARC+ slow-release fertilizer on leguminous crops such as soybeans and peanuts

[0691] The eight ARC+ controlled-release fertilizers 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans during sowing. Fields treated with the same amount of controlled-release fertilizers served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the controlled-release fertilizers in the control fields performed their normal function. Compared to the control fields, the eight ARC+ controlled-release fertilizers 1-8 significantly promoted nodulation and nitrogen fixation, with nodule number increasing by more than 2.69 times, nitrogenase activity per plant increasing by more than 7.55 times, and yield per unit area increasing by more than 12%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the above examples.

[0692] The above results show that ARC microbial agent can be used to produce ARC+ slow-release fertilizer, and ARC+ slow-release fertilizer can have the effects of slow-release fertilizer and ARC microbial agent at the same time.

[0693] The use of ARC microbial agent in the production of ARC+ bio-organic fertilizer and its application in leguminous crops such as soybeans and peanuts

[0694] Example 66: Use of ARC microbial agent - for producing ARC+ bio-organic fertilizer

[0695] The ARC microbial agents 1-8 are mixed with the conventional amount of bio-organic fertilizer per mu of land at a dosage of no less than 80 billion viable bacteria per mu of land. The ARC microbial agents are then physically adsorbed and fixed onto the bio-organic fertilizer to prepare eight types of ARC + bio-organic fertilizers 1-8. The bio-organic fertilizers can be purchased directly from the market.

[0696] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the bio-organic fertilizer particles for adsorption.

[0697] Before the above-mentioned ARC microbial agent spraying and adsorption operation, a microbial protective agent can be sprayed and adsorbed on the surface of the biological organic fertilizer particles.

[0698] The above-mentioned microbial protective agent can be inorganic carbon, sulfur or biological organic matter and other materials, which can be purchased from the market.

[0699] Example 67: Application of ARC+ bio-organic fertilizer on leguminous crops such as soybeans and peanuts

[0700] The eight ARC+ bio-organic fertilizers 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans. Fields treated with the same amount of bio-organic fertilizers served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the bio-organic fertilizers in the control fields performed their normal function. Compared to the control fields, the eight ARC+ bio-organic fertilizers 1-8 significantly promoted nodulation and nitrogen fixation, with nodule number increasing by more than 2.56 times, nitrogenase activity per plant increasing by more than 7.35 times, and yield per unit area increasing by more than 11.7%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial inoculants described in the above examples.

[0701] The above results show that ARC microbial agent can be used to produce ARC+ bio-organic fertilizer, and ARC+ bio-organic fertilizer can have the effects of both bio-organic fertilizer and ARC microbial agent.

[0702] The use of ARC microbial agent in the production of ARC+ water-soluble fertilizer and its application in leguminous crops such as soybeans and peanuts

[0703] Example 68: Use of ARC microbial agent - Use for producing ARC+ water-soluble fertilizer

[0704] ARC microbial agents 1-8 are mixed at a rate of no less than 80 billion viable bacteria per mu (approximately 100 million acres) with water-soluble fertilizers at a rate of 1-8 per mu (approximately 100 million acres). The ARC microbial agents and water-soluble fertilizers are then uniformly blended using conventional physical methods to create eight ARC+water-soluble fertilizers. The water-soluble fertilizers are commercially available or prepared using conventional mixing methods.

[0705] The eight ARC+water-soluble fertilizers 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same water-soluble fertilizers served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the eight ARC+water-soluble fertilizers 1-8 significantly promoted nodulation and nitrogen fixation, increasing nodule number by more than threefold, nitrogenase activity per plant by more than tenfold, and yield per unit area by more than 15.5%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the examples above.

[0706] The above results indicate that ARC microbial agent can be used to prepare ARC+water-soluble fertilizer, which has the effects of both water-soluble fertilizer and ARC microbial agent.

[0707] The use of ARC microbial agent in the production of ARC+ organic-inorganic compound fertilizer and its application in leguminous crops such as soybeans and peanuts

[0708] Example 69: Use of ARC microbial agent - for the production of ARC + organic-inorganic compound fertilizer

[0709] The ARC microbial agents 1-8 are mixed with the conventional amount of organic-inorganic compound fertilizer per mu of land at a dosage of no less than 80 billion viable bacteria per mu of land. The ARC microbial agents are then physically adsorbed and fixed onto the organic-inorganic compound fertilizer to prepare eight types of ARC + organic-inorganic compound fertilizers 1-8. The organic-inorganic compound fertilizers can be purchased directly from the market.

[0710] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the organic-inorganic compound fertilizer particles for adsorption.

[0711] Before the above-mentioned ARC microbial agent spraying and adsorption operation, a microbial protective agent can be sprayed and adsorbed on the surface of the organic-inorganic compound fertilizer particles.

[0712] The above-mentioned microbial protective agent can be inorganic carbon, sulfur or biological organic matter and other materials, which can be purchased from the market.

[0713] Example 70: Application of ARC+ organic-inorganic compound fertilizer on leguminous crops such as soybeans and peanuts

[0714] The eight ARC+organic-inorganic compound fertilizers 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans. Fields treated with the same amount of organic-inorganic compound fertilizers served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the organic-inorganic compound fertilizers in the control fields performed their normal function. Compared to the control fields, the eight ARC+organic-inorganic compound fertilizers 1-8 significantly promoted nodulation and nitrogen fixation, with nodule number increasing by more than 2.79 times, nitrogenase activity per plant increasing by more than 7.94 times, and yield per unit area increasing by more than 12.2%, achieving a significant yield increase and demonstrating the key characteristics of the ARC microbial inoculants described in the above examples.

[0715] The above results show that ARC microbial agent can be used to produce ARC+organic-inorganic compound fertilizer, and ARC+organic-inorganic compound fertilizer can have the effects of both organic-inorganic compound fertilizer and ARC microbial agent.

[0716] The use of ARC microbial agent in the production of ARC+ rhizobium fertilizer (agent) and its application in leguminous crops such as soybeans and peanuts

[0717] Example 71: Use of ARC Microbial Agent - Use for Production of ARC+Rhizobium Fertilizer (Agent) (I)

[0718] The ARC microbial agents 1-8 are mixed with the rhizobium fertilizer (agent) at a dosage of no less than 80 billion viable bacteria per mu of land, and the dosage of the rhizobium fertilizer (agent) per mu of land is then mixed uniformly with the ARC microbial agents 1-8 using conventional physical methods to prepare eight types of ARC+rhizobium fertilizers (agents) 1-8. The rhizobium fertilizers (agents) can be purchased commercially or prepared by isolating, identifying, and fermenting leguminous crop nodules using conventional methods.

[0719] The eight ARC+Rhizobium fertilizers (agents) 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same Rhizobium fertilizers (agents) served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the eight ARC+Rhizobium fertilizers (agents) 1-8 significantly promoted nodulation and nitrogen fixation, increasing nodule number by more than 3.5 times, nitrogenase activity per plant by more than 10.5 times, and yield per unit area by more than 15.0%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the above examples.

[0720] The above results indicate that ARC microbial agent can be used to prepare ARC+rhizobium fertilizer (agent), which has the effects of both rhizobium fertilizer (agent) and ARC microbial agent.

[0721] Example 72: Use of ARC Microbial Agent - Use in the Production of ARC + Rhizobium Fertilizer (Agent) (II)

[0722] The above-mentioned ARC microbial agents 1 to 8 are respectively proportioned with the amount of rhizobium fertilizer (agent) per mu of land according to a dosage of not less than 80 billion viable bacteria per mu of land and ARC microbial agents 1 to 8, the bacterial liquid / powder of the rhizobium fertilizer (agent) and the bacterial liquid / powder of the ARC microbial agent are uniformly mixed by conventional physical methods, and then adsorbed onto a carrier by a physical method to prepare ARC+rhizobium fertilizer (agent).

[0723] The above-mentioned rhizobium fertilizer (agent), rhizobium liquid and rhizobium powder are directly purchased from the market, or can be prepared by separation, identification, screening and fermentation from leguminous crop nodules using conventional methods.

[0724] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the carrier particles for adsorption.

[0725] The carrier can be a mixture of carbon powder and humus at a ratio of 9:1, or a mixture of one or two or more of carbon powder, carbon powder, humus, zeolite powder, diatomaceous earth, vermiculite, bentonite, weathered coal, etc.

[0726] The eight ARC+Rhizobium fertilizers (agents) 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same Rhizobium fertilizers (agents) served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the eight ARC+Rhizobium fertilizers (agents) 1-8 significantly promoted nodulation and nitrogen fixation, increasing nodule number by more than 4.1 times, nitrogenase activity per plant by more than 12.7 times, and yield per unit area by more than 16.0%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the above examples.

[0727] The above results indicate that ARC microbial agent can be used to prepare ARC+rhizobium fertilizer (agent), which has the effects of both rhizobium fertilizer (agent) and ARC microbial agent.

[0728] The use of ARC microbial agent in the production of ARC+ compound microbial fertilizer and its application in leguminous crops such as soybeans and peanuts

[0729] Example 73: Use of ARC microbial agent - for the production of ARC+ compound microbial fertilizer

[0730] The ARC microbial agents 1-8 are mixed with the conventional amount of compound microbial fertilizer per mu of land at a dosage of no less than 80 billion viable bacteria per mu of land. The ARC microbial agents are then physically adsorbed and fixed onto the compound microbial fertilizer to prepare eight types of ARC + compound microbial fertilizers 1-8. The compound microbial fertilizers can be purchased directly from the market.

[0731] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the composite microbial fertilizer particles for adsorption.

[0732] Before the above-mentioned ARC microbial agent spraying and adsorption operation, a microbial protective agent can be sprayed and adsorbed on the surface of the compound microbial fertilizer particles.

[0733] The above-mentioned microbial protective agent can be inorganic carbon, sulfur or biological organic matter and other materials, which can be purchased from the market.

[0734] Example 74: Application of ARC+ compound microbial fertilizer on leguminous crops such as soybeans and peanuts

[0735] The eight ARC+compound microbial fertilizers 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans. Fields treated with the same amount of compound microbial fertilizers served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the compound microbial fertilizers in the control fields performed their normal function. Compared to the control fields, the eight ARC+compound microbial fertilizers 1-8 significantly promoted nodulation and nitrogen fixation, with nodule numbers increasing by more than three times, nitrogenase activity per plant increasing by more than eight times, and yield per unit area increasing by more than 11%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the above examples.

[0736] The above results show that ARC microbial agent can be used to produce ARC+compound microbial fertilizer, and ARC+compound microbial fertilizer can have the effects of both compound microbial fertilizer and ARC microbial agent.

[0737] The use of ARC microbial agent in the production of ARC+ fertilizer synergist or fertilizer adjuvant and its application in leguminous crops such as soybeans and peanuts

[0738] Example 75: Use of ARC Microbial Agent - Use for Producing ARC+ Fertilizer Synergist or Fertilizer Adjuvant (I)

[0739] The ARC microbial agents 1-8 are mixed at a dosage of no less than 80 billion viable bacteria per mu (approximately 100 million acres) with a fertilizer synergist or fertilizer adjuvant at a dosage of 1-8. The ARC microbial agents and fertilizer synergist or fertilizer adjuvant are then uniformly blended using conventional physical methods to create eight types of ARC + fertilizer synergist or fertilizer adjuvants 1-8. The fertilizer synergist or fertilizer adjuvant can be purchased commercially or prepared by conventional methods by isolating, identifying, and fermenting leguminous crop nodules.

[0740] The eight ARCs plus fertilizer synergists or fertilizer adjuvants 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same fertilizer synergists or fertilizer adjuvants served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the application of the eight ARCs plus fertilizer synergists or fertilizer adjuvants 1-8, in addition to achieving the effects of the fertilizer synergists or fertilizer adjuvants, significantly promoted nodulation and nitrogen fixation. The number of nodules increased by more than 2-fold, the nitrogenase activity per plant increased by more than 6-fold, and the yield per unit area increased by more than 10%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial inoculants described in the above examples.

[0741] The above results indicate that ARC microbial agent can be used to prepare ARC+fertilizer synergist or fertilizer adjuvant, and has the effects of fertilizer synergist or fertilizer adjuvant and ARC microbial agent at the same time.

[0742] Example 76: Use of ARC Microbial Agent - Use in the Production of ARC+ Fertilizer Synergist or Fertilizer Adjuvant (II)

[0743] The above-mentioned ARC microbial agents 1 to 8 are mixed in proportion with the amount of fertilizer synergist or fertilizer adjuvant per mu of land at a dosage of not less than 80 billion viable bacteria per mu of land, and the ARC microbial agents are adsorbed onto the fertilizer synergist or fertilizer adjuvant particles by a physical method to prepare ARC + fertilizer synergist or fertilizer adjuvant.

[0744] The above-mentioned fertilizer synergists or fertilizer adjuvants can be purchased from the market.

[0745] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the fertilizer synergist or fertilizer adjuvant particles for adsorption.

[0746] The eight ARC+ fertilizer synergists or fertilizer adjuvants 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same fertilizer synergists or fertilizer adjuvants served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the application of the eight ARC+ fertilizer synergists or fertilizer adjuvants 1-8 not only achieved soil conditioning effects but also significantly promoted nodulation and nitrogen fixation. Nodule number increased by more than 2.5 times, nitrogenase activity per plant increased by more than 7.5 times, and yield per unit area increased by more than 11%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial inoculants described in the above examples.

[0747] The above results indicate that ARC microbial agent can be used to prepare ARC+fertilizer synergist or fertilizer adjuvant, and has the effects of fertilizer synergist or fertilizer adjuvant and ARC microbial agent at the same time.

[0748] The use of ARC microbial agent in the production of ARC+ biocontrol agent and its application in leguminous crops such as soybeans and peanuts

[0749] Example 77: Use of ARC microbial agent - Use for producing ARC+ biocontrol agent (I)

[0750] The ARC microbial agents 1-8 are mixed with the biocontrol agent at a dosage of no less than 80 billion viable bacteria per mu (approximately 100 million acres) and the dosage of the biocontrol agent per mu (approximately 100 million acres). The ARC microbial agents and the biocontrol agent are then uniformly blended using conventional physical methods to form eight types of ARC + biocontrol agents 1-8. These biocontrol agents can be purchased commercially or prepared by conventional methods by isolating, identifying, and fermenting leguminous crop nodules.

[0751] The above-mentioned biocontrol agent is Trichoderma harzianum with biocontrol effect, which can be directly purchased from the market, or can be prepared by isolating, identifying, screening and fermenting from nodules of leguminous crops through conventional methods.

[0752] The eight ARC+ biocontrol agents 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same biocontrol agents served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the eight ARC+ biocontrol agents 1-8 significantly promoted nodulation and nitrogen fixation, increasing nodule number by more than 2.7 times, increasing nitrogenase activity per plant by more than 10 times, and increasing yield per unit area by more than 10%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the examples above.

[0753] The above results indicate that ARC microbial agent can be used to prepare ARC+biocontrol agent, and has the effects of both biocontrol agent and ARC microbial agent.

[0754] Example 78: Use of ARC microbial agent - Use for producing ARC+ biocontrol agent (II)

[0755] The above-mentioned ARC microbial agents 1 to 8 are mixed in proportion with the amount of biocontrol agents per mu of land at a dosage of not less than 100 billion viable bacteria per mu, and the bacterial liquid / powder of the biocontrol agents and the bacterial liquid / powder of the ARC microbial agents are uniformly mixed by conventional physical methods, and then adsorbed onto a carrier by physical methods to prepare an ARC+ biocontrol agent.

[0756] The above-mentioned biocontrol agent is a Bacillus with biocontrol effect, which can be directly purchased from the market, or can be prepared by isolating, identifying, screening and fermenting from nodules of leguminous crops through conventional methods.

[0757] The physical method can use a spray gun to spray a mixed bacterial liquid consisting of liquid ARC microbial agent and biocontrol agent or a mixed bacterial powder consisting of dry powder ARC microbial agent and biocontrol agent onto the carrier particles.

[0758] The carrier can be a mixture of carbon powder and humus at a ratio of 9:1, or a mixture of one or two or more of carbon powder, carbon powder, humus, zeolite powder, diatomaceous earth, vermiculite, bentonite, weathered coal, etc.

[0759] The eight ARC+ biocontrol agents 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same biocontrol agents served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the eight ARC+ biocontrol agents 1-8 significantly promoted nodulation and nitrogen fixation, increasing nodule number by more than 2.5 times, nitrogenase activity per plant by more than 9.6 times, and yield per unit area by more than 12.7%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the above examples.

[0760] Example 79: Use of ARC Microbial Agent - Use for Producing ARC+ Biocontrol Agent (III)

[0761] The above-mentioned ARC microbial agents 1 to 8 are mixed in proportion with the amount of biocontrol agents per mu of land at a dosage of not less than 100 billion viable bacteria per mu, and the bacterial liquid / powder of the biocontrol agents and the bacterial liquid / powder of the ARC microbial agents are uniformly mixed by conventional physical methods, and then adsorbed onto a carrier by physical methods to prepare an ARC+ biocontrol agent.

[0762] The above-mentioned biocontrol agent is Beauveria bassiana with biocontrol effect, which can be directly purchased from the market, or can be obtained by isolating, identifying, screening and fermenting from nodules of leguminous crops through conventional methods.

[0763] The physical method can use a spray gun to spray a mixed bacterial liquid consisting of liquid ARC microbial agent and biocontrol agent or a mixed bacterial powder consisting of dry powder ARC microbial agent and biocontrol agent onto the carrier particles.

[0764] The carrier can be a mixture of carbon powder and humus at a ratio of 9:1, or a mixture of one or two or more of carbon powder, carbon powder, humus, zeolite powder, diatomaceous earth, vermiculite, bentonite, weathered coal, etc.

[0765] The eight ARC+ biocontrol agents 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same biocontrol agents served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the eight ARC+ biocontrol agents 1-8 significantly promoted nodulation and nitrogen fixation, increasing nodule number by more than 2.2 times, nitrogenase activity per plant by more than 7.3 times, and yield per unit area by more than 10%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the examples above.

[0766] The above results indicate that ARC microbial agent can be used to prepare ARC+biocontrol agent, and has the effects of both biocontrol agent and ARC microbial agent.

[0767] The use of ARC microbial agent in the production of ARC+ soil conditioner and its application in leguminous crops such as soybeans and peanuts

[0768] Example 80: Use of ARC Microbial Agent - Use for Producing ARC+ Soil Conditioner (I)

[0769] The ARC microbial agents 1-8 are mixed with soil conditioners at a dosage of no less than 80 billion viable bacteria per mu (approximately 100 million acres) and the dosage of soil conditioners per mu (approximately 100 million acres). The ARC microbial agents and soil conditioners are then uniformly blended using conventional physical methods to create eight ARC + soil conditioners 1-8. These soil conditioners can be purchased commercially or prepared by conventional methods by isolating, identifying, and fermenting leguminous crop nodules.

[0770] The eight ARC+ soil conditioners 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same soil conditioners served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the application of the eight ARC+ soil conditioners 1-8, in addition to achieving the same soil conditioner effects, significantly promoted nodulation and nitrogen fixation, increasing nodule number by more than 2.5 times, nitrogenase activity per plant by more than 7.7 times, and yield per unit area by more than 9.3%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the examples above.

[0771] The above results indicate that ARC microbial agent can be used to prepare ARC+ soil conditioner, and has the effects of both soil conditioner and ARC microbial agent.

[0772] Example 81: Use of ARC Microbial Agent - Use for Producing ARC+ Soil Conditioner (II)

[0773] The above-mentioned ARC microbial agents 1 to 8 are mixed in proportion with the amount of soil conditioner per mu according to a dosage of not less than 80 billion viable bacteria per mu, and the ARC microbial agents are adsorbed onto the soil conditioner particles by a physical method to prepare ARC+ soil conditioner.

[0774] The above-mentioned soil conditioners can be purchased from the market.

[0775] The above physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the soil conditioner particles for adsorption.

[0776] The eight ARC+ soil conditioners 1-8 were used as seed fertilizers at sowing on leguminous crops such as peanuts and soybeans. Fields treated with the same soil conditioners served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the application of the eight ARC+ soil conditioners 1-8, in addition to achieving soil conditioning effects, significantly promoted nodulation and nitrogen fixation, increasing nodule number by over 2.6 times, nitrogenase activity per plant by over 9.5 times, and yield per unit area by over 10%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the examples above.

[0777] The above results indicate that ARC microbial agent can be used to prepare ARC+ soil conditioner, and has the effects of both soil conditioner and ARC microbial agent.

[0778] The use of ARC microbial agent in the production of ARC+ water-retaining agent and its application in leguminous crops such as soybeans and peanuts

[0779] Example 82: Use of ARC microbial agent - Use for producing ARC+ water-retaining agent

[0780] The ARC microbial agents 1-8 are mixed at a dosage of no less than 80 billion viable bacteria per mu (approximately 100 million acres) with the water-retaining agent (approximately 100 million acres) per mu (approximately 100 million acres). The ARC microbial agents are then adsorbed and fixed onto the water-retaining agent particles using conventional physical methods to create eight types of ARC + water-retaining agents 1-8. The water-retaining agents are commercially available.

[0781] The conventional physical method can use a spray gun to spray the ARC microbial agent in liquid state or the ARC microbial agent in dry powder state onto the water retaining agent particles for adsorption.

[0782] The eight ARC+water-retaining agents 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans at sowing. Fields treated with the same water-retaining agents served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the application of the eight ARC+water-retaining agents 1-8, in addition to achieving the desired water-retaining effect, significantly promoted nodulation and nitrogen fixation, increasing nodule number by over 3.22 times, nitrogenase activity per plant by over 10.6 times, and yield per unit area by over 11.5%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial inoculants described in the aforementioned examples.

[0783] The above results indicate that ARC microbial agent can be used to prepare ARC+water-retaining agent, and has the effects of both water-retaining agent and ARC microbial agent.

[0784] The use of ARC microbial agent in the production of ARC+ pesticide and its application in leguminous crops such as soybeans and peanuts

[0785] Example 83: Use of ARC microbial agent - Use for producing ARC+pesticide

[0786] The ARC microbial agents 1-8 are mixed at a dosage of no less than 100 billion viable bacteria per mu (approximately 100 billion active bacteria per mu) and the pesticide dosage per mu (approximately 100 billion active bacteria per mu). The ARC microbial agents and the pesticides are then uniformly mixed using conventional physical methods to prepare eight types of ARC + pesticides 1-8. The pesticides are herbicides and can be purchased commercially.

[0787] The eight ARC+pesticides 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans during sowing. Fields treated with the same pesticides served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the application of the eight ARC+pesticides 1-8, while achieving the desired pesticide effects, also significantly promoted nodulation and nitrogen fixation. Nodule number increased by more than 2.2 times, nitrogenase activity per plant increased by more than 6.9 times, and yield per unit area increased by more than 6.8%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the examples above.

[0788] The above results indicate that ARC microbial agent can be used to prepare ARC+pesticide, and has the effects of both pesticide and ARC microbial agent.

[0789] Example 84: Use of ARC microbial agent - Use for producing ARC+pesticide

[0790] The ARC microbial agents 1-8 were mixed at a dosage of no less than 100 billion viable bacteria per mu (approximately 100 billion active bacteria per mu) and the pesticide dosage per mu (approximately 100 billion active bacteria per mu). The ARC microbial agents and the pesticides were then uniformly blended using conventional physical methods to create eight ARC+pesticides 1-8. The pesticides were a mixture of an insecticide and a plant growth regulator, all of which were purchased commercially.

[0791] The eight ARC+pesticides 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans during sowing. Fields treated with the same pesticides served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the application of the eight ARC+pesticides 1-8, while not only exerting their pesticide effects, also significantly promoted nodulation and nitrogen fixation. Nodule number increased by more than 2.1 times, nitrogenase activity per plant increased by more than 5.8 times, and yield per unit area increased by more than 6.5%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the examples above.

[0792] The above results indicate that ARC microbial agent can be used to prepare ARC+pesticide, and has the effects of both pesticide and ARC microbial agent.

[0793] Example 85: Use of ARC microbial agent - Use for producing ARC+pesticide

[0794] The ARC microbial agents 1-8 are mixed at a dosage of no less than 100 billion viable bacteria per mu (approximately 100 billion active bacteria per mu) and the dosage of the pesticide per mu (approximately 100 billion active bacteria per mu). The ARC microbial agents and the pesticide are then uniformly mixed using conventional physical methods to prepare eight types of ARC + pesticides 1-8. The pesticides can be insecticides, which can be purchased commercially.

[0795] The eight ARC+pesticides 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans during sowing. Fields treated with the same pesticides served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the application of the eight ARC+pesticides 1-8 not only achieved the desired pesticide effects but also significantly promoted nodulation and nitrogen fixation. Nodule number increased by more than 1.7 times, nitrogenase activity per plant increased by more than 4.9 times, and yield per unit area increased by more than 6.3%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the examples above.

[0796] The above results indicate that ARC microbial agent can be used to prepare ARC+pesticide, and has the effects of both pesticide and ARC microbial agent.

[0797] The use of ARC microbial agent in the production of ARC+ seed coating agent, seed dressing agent or seed soaking agent and its application in leguminous crops such as soybeans and peanuts

[0798] Example 86: Use of ARC microbial agent - Use for producing ARC+ seed coating agent

[0799] The ARC microbial agents 1-8 are mixed with the seed dressing agent at a dosage of no less than 80 billion viable bacteria per mu (approximately 100 million acres) and the dosage of the seed dressing agent. The ARC microbial agents and the seed dressing agent are then uniformly blended using conventional physical methods to prepare eight types of ARC + seed dressing agents 1-8. The seed dressing agents can be purchased commercially.

[0800] The eight ARC+seed coating agents 1-8 were used as seed fertilizers at sowing on leguminous crops such as peanuts and soybeans. Fields treated with the same seed coating agents served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the application of the eight ARC+seed coating agents 1-8, while achieving the desired effects as seed coating agents, also significantly promoted nodulation and nitrogen fixation. Nodule number increased by more than 2-fold, nitrogenase activity per plant increased by more than 6-fold, and yield per unit area increased by more than 9%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the examples above.

[0801] The above results indicate that ARC microbial agent can be used to prepare ARC+seed dressing agent, and has the effects of both seed dressing agent and ARC microbial agent.

[0802] Example 87: Use of ARC microbial agent - Use for producing ARC+ seed dressing

[0803] The ARC microbial agents 1-8 were mixed with the seed dressing agent at a dosage of no less than 80 billion viable bacteria per mu of land, and then uniformly blended with the seed dressing agent using conventional physical methods to prepare eight types of ARC + seed dressing agents 1-8. The seed dressing agents were purchased from the market.

[0804] The eight ARC+ seed dressings 1-8 were used as seed fertilizers at sowing in leguminous crops such as peanuts and soybeans. Fields treated with the same seed dressings served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the application of the eight ARC+ seed dressings 1-8, while not only functioning as seed dressings, also significantly promoted nodulation and nitrogen fixation. Nodule number increased by more than 2.4 times, nitrogenase activity per plant increased by more than 6.6 times, and yield per unit area increased by more than 9.4%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the examples above.

[0805] The above results indicate that ARC microbial agent can be used to prepare ARC+seed dressing agent, and has the effects of both seed dressing agent and ARC microbial agent.

[0806] Example 88: Use of ARC microbial agent - Use for producing ARC+ seed soaking agent

[0807] The ARC microbial agents 1-8 are mixed with the seed soaking agent at a dosage of no less than 80 billion viable bacteria per mu of land, and then mixed evenly with the ARC microbial agents using conventional physical methods to prepare eight types of ARC + seed soaking agents 1-8. The seed soaking agents can be purchased commercially.

[0808] The eight ARC+seed soaking agents 1-8 were used as seed fertilizers in leguminous crops such as peanuts and soybeans during sowing. Fields treated with the same seed soaking agents served as controls, and ongoing surveys were conducted after sowing. These survey results showed that the application of the eight ARC+seed soaking agents 1-8 not only achieved the same benefits as the seed soaking agents but also significantly promoted nodulation and nitrogen fixation. Nodule number increased by more than 2.9 times, nitrogenase activity per plant increased by more than 8.6 times, and yield per unit area increased by more than 9.1%, achieving significant yield increases and demonstrating the key characteristics of the ARC microbial agents described in the examples above.

[0809] The above results indicate that ARC microbial agent can be used to prepare ARC+seed soaking agent, and has the effects of both seed soaking agent and ARC microbial agent.

Claims

1. ARC microbial agent for controlling toxicity and fixing nitrogen coupled with increasing production, characterized by: It is a microbial composition with a coupled effect of toxicity control and nitrogen fixation, and has the function of regulating and increasing the abundance of rhizobia in the rhizosphere of leguminous crops and increasing the number of nodules in leguminous crops. It contains the DNA sequences shown in SEQ ID No. 1-4.

2. The ARC microbial agent according to claim 1, characterized in that: The ARC microbial agent has an inhibitory effect on aflatoxin and / or its toxins.

3. The ARC microbial agent according to claim 1, characterized in that: The ARC microbial agent has a significant inhibitory effect on the expression of Aspergillus flavus PAB-01 protein, with an inhibition rate of more than 90%. The amino acid sequence of the PAB-01 protein is shown in SEQ ID No.

5.

4. The ARC microbial agent according to claim 1, characterized in that: The ARC microbial agent has an inhibitory effect on one or more pathogens / toxins of Aspergillus other than Penicillium and Aspergillus flavus, Fusarium, Sclerotium sclerotiorum, Pseudomonas solanacearum, Phytophthora, Sclerotinia sclerotiorum, Pythium, and Rhizoctonia soil-borne plant pathogens.

5. The ARC microbial agent according to claim 1, characterized in that: The DNA sequence genes shown in SEQ ID No. 1-4 may have a certain degree of variation in different strains. When the degree of variation is small, not exceeding 10% of the base variation and having the corresponding biological activity function, they constitute functional equivalents of the DNA sequences shown in SEQ ID No. 1-4. The microbial composition contains all the gene sequences shown in SEQ ID NO. 1 to 4 or their functional equivalents, and has a coupled effect of toxic control and nitrogen fixation, and has the effect of regulating and increasing the abundance of rhizobia in the rhizosphere of leguminous crops and increasing the number of nodules of leguminous crops, constituting the ARC microbial agent according to claim 1.

6. The ARC microbial agent according to claim 1, characterized in that: The ARC microbial agent is a composition of the following four strains of microorganisms: Bacillus laterosporus with a preservation number of CCTCC NO: M 20231815, Bacillus amyloliquefaciens with a preservation number of CCTCC NO: M 20231598, Bacillus mucilaginosus with a preservation number of CCTCC NO: M 20231817, and Enterobacter ludwigii with a preservation number of CCTCC NO: M 20231595.

7. The ARC microbial agent according to claim 1, characterized in that: The ARC microbial agent is a combination of one or more microorganisms selected from Bacillus laterosporus with a deposit number of CCTCC NO: M 20231815, Bacillus amyloliquefaciens with a deposit number of CCTCC NO: M 20231598, Bacillus mucilaginosus with a deposit number of CCTCC NO: M 20231817, and Enterobacter ludwigii with a deposit number of CCTCC NO: M 20231595, and other microorganisms, so that the combined microbial agent satisfies the requirements of containing all gene sequences in the nucleotide sequences shown in SEQ ID NOs. 1 to 4 or their functional equivalents, has a coupled effect of toxicity control and nitrogen fixation, and has the effect of regulating and increasing the abundance of rhizobia in the rhizosphere of leguminous crops and increasing the number of nodules in leguminous crops, thereby constituting the ARC microbial agent of claim 1.

8. The ARC microbial agent according to claim 1, characterized in that: In the ARC microbial agent, the proportion of viable bacteria of any one strain in the microbial agent is greater than or equal to 1%.

9. A method for crop production, characterized in that: The microbial agent is selected so that, after analysis and determination, the microbial agent contains all the gene sequences in the nucleotide sequences shown in SEQ ID NOs. 1 to 4, and has a coupled effect of controlling toxicity and fixing nitrogen, and has the effect of regulating and increasing the abundance of rhizobia in the rhizosphere of leguminous crops and increasing the number of nodules in leguminous crops; the microbial agent is applied to crops to control toxicity and fix nitrogen, improve quality and increase yield.

10. The method according to claim 9, characterized in that: The DNA sequence genes shown in SEQ ID No. 1-4 may have a certain degree of variation in different strains. When the degree of variation is small, not exceeding 10% of the base variation and having the corresponding biological activity function, the functional equivalents of the DNA sequences shown in SEQ ID No. 1-4 contain all the gene sequences shown in SEQ ID NO. 1 to 4 or their functional equivalents, and have a coupled effect of controlling toxicity and fixing nitrogen. It has the effect of regulating and increasing the abundance of rhizobia in the rhizosphere of leguminous crops and increasing the number of nodules in leguminous crops. It is an ARC microbial agent for coupling controlling toxicity and fixing nitrogen and increasing yield, which can be used in crop production for controlling toxicity and fixing nitrogen, improving quality and increasing yield.

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