Composition for enhancing atpase activity and supplementing ATP, and preparation method therefor

Abstract

A composition for enhancing ATPase activity and supplementing ATP, and a preparation method therefor and the use thereof. The composition comprises the following raw materials: Poria cocos, Rosae rugosae flos, Lycii fructus, Citri reticulatae pericarpium, Morus alba fructus, Jujubae fructus, Polygonati odorati rhizoma, Dimocarpus longan, Polygonati rhizoma and Asini corii colla. The method for preparing the composition product comprises the following steps: (1) selecting one or several raw materials in a formulation, separately soaking the raw materials in a solvent of 5-30 times the amount of the raw materials for 10 min to 5 h, and then performing heating extraction 1-4 times, each time for 10 min to 5 h, so as to obtain extracts; (2) combining the extracts, and concentrating same into a thick paste; and (3) dissolving Asini corii colla by adding an appropriate amount of water, and uniformly mixing same with the thick paste. The product is used for improving glucose uptake, enhancing ATPase activity and increasing cellular ATP content.

Description

A composition for enhancing ATPase activity and supplementing ATP and its preparation method Technical Field

[0001] The present invention relates to the field of food, and in particular to a composition for enhancing ATPase activity and supplementing ATP, and a preparation method thereof. Background Art

[0002] Traditional Chinese Medicine (TCM) places great emphasis on the role of visceral function and essence and qi in its understanding of the causes of aging. Kidney yang deficiency, spleen and stomach deficiency, and insufficient essence and qi are among the mainstream theories of aging in TCM. TCM considers the kidneys to be the foundation of innate constitution, storing essence and qi. The strength and weakness of the kidneys influence the strength and weakness of vital energy and biochemical function. The discussion of "a woman's 77th birthday" and "a man's 88th birthday" in the Suwen (Suwen) Treatise on Ancient Innocence uses the natural rise and fall of kidney qi to illustrate the relationship between the growth, development, and aging processes of the human body and innate constitution, suggesting that the key to aging lies in the strength and weakness of kidney qi. TCM considers the spleen and stomach to be the foundation of acquired constitution, with food and drink entering the stomach and the five internal organs receiving qi from the stomach. If the spleen and stomach are deficient, food and drink cannot be digested and absorbed, and the body's nutrients are not replenished in a timely manner, which can affect health and accelerate aging. Essence and qi are divided into innate essence and acquired essence. The Yellow Emperor's Classic of Internal Medicine explains that innate qi, also known as "primordial qi" or "kidney qi," originates from the innate essence in the kidneys. Acquired essence qi, the various refined substances transformed from food and drink, originates from the spleen and stomach after birth. The remaining portion of acquired essence qi, after balanced metabolism, is ultimately stored in the kidneys, becoming part of kidney essence.

[0003] The essence of aging is cellular decay. A research review titled "The Hallmarks of Aging" published in the top international journal Cell attributes aging to nine factors, including cellular senescence, stem cell depletion, and mitochondrial dysfunction—the famous "Nine-Dimensional Cell Theory." This research brings scientific aging prevention down to the cellular level. The study also divides human aging into three age groups: after 25, between 30 and 40, and over 40. It concludes that these three age groups are the most critical stages of aging, and that human intervention within these three age groups can slow the rate of aging.

[0004] Metabolism refers to the exchange of substances and energy between the body and the external environment, as well as the process of self-renewal of substances and energy within an organism. It is one of the most fundamental characteristics of life and encompasses both material metabolism and energy metabolism. Through material metabolism, the body absorbs nutrients from the outside world, breaks them down and absorbs them internally, releasing the chemical energy stored in them and converting it into energy that can be used by tissues and cells to maintain normal life activities.

[0005] Mitochondria are essential organelles in eukaryotic cells, key sites of oxidative metabolism and the production of ATP. They are known as the "energy factories of the cell," and their activity directly reflects the cell's metabolic capacity. ATP, or adenosine triphosphate, is a high-energy phosphate compound ubiquitous in all living cells. It serves as a direct energy source within the cell and is often referred to as the "energy currency of the cell." When ATP is hydrolyzed by ATPase, ATP releases a large amount of energy, powering chemical reactions such as cellular metabolism. When ATP is insufficient or ATPase activity is low, the body cannot obtain sufficient energy, leading to organ dysfunction, decreased metabolism, decreased motor function, increased toxin accumulation, and accelerated aging. To replenish ATP, we need to increase glucose intake and enhance the activity of transport proteins; otherwise, glucose cannot enter the cell and be broken down into ATP.

[0006] Currently, there is little research on enhancing cellular ATPase activity or increasing cellular ATP content. Therefore, existing therapeutic products and technologies need to be improved and developed. Summary of the Invention

[0007] Based on the research results of the aging mechanisms of traditional Chinese medicine and Western medicine, according to the sub-health targets of women, this invention uses modern scientific research technology to screen out single Chinese herbal medicines with high activity from 10 top-grade medicinal materials including rose, polygonatum, donkey-hide gelatin, wolfberry, tangerine peel, mulberry, jujube, polygonatum, longan, and poria, and integrates the aging theory of traditional Chinese medicine with modern medical technology to develop a formula qualitatively and quantitatively.

[0008] In some embodiments, the present invention provides a composition, characterized in that the composition comprises the following raw materials: Poria cocos, rose, wolfberry, tangerine peel, mulberry, jujube, polygonatum, longan, polygonatum, and donkey-hide gelatin; and the composition is prepared by processing the raw materials.

[0009] In some embodiments, the present invention provides a composition, characterized in that the composition comprises the following raw materials in parts by weight: 1-60 parts of Poria cocos, 1-40 parts of rose, 1-40 parts of wolfberry, 1-40 parts of tangerine peel, 1-40 parts of mulberry, 1-40 parts of jujube, 1-40 parts of Polygonatum odoratum, 1-30 parts of longan, 1-30 parts of Polygonatum sibiricum and 1-20 parts of donkey-hide gelatin.

[0010] In some embodiments, the present invention provides a composition, characterized in that the composition comprises the following raw materials in parts by weight: 1-60 parts of Poria cocos, 1-40 parts of rose, 1-40 parts of wolfberry, 1-40 parts of dried tangerine peel, 1-40 parts of mulberry, 1-40 parts of jujube, 1-40 parts of Polygonatum odoratum, 1-20 parts of longan, 1-20 parts of Polygonatum sibiricum and 1-10 parts of donkey-hide gelatin.

[0011] In some embodiments, the composition includes the following raw materials in parts by weight: 3-60 parts of Poria cocos, 2-40 parts of rose, 2-40 parts of wolfberry, 2-40 parts of dried tangerine peel, 2-40 parts of mulberry, 2-40 parts of jujube, 2-40 parts of Polygonatum odoratum, 2-20 parts of longan, 2-20 parts of Polygonatum sibiricum and 1-10 parts of donkey-hide gelatin.

[0012] In some embodiments, the composition includes the following raw materials in parts by weight: 9-40 parts of Poria cocos, 6-25 parts of rose, 6-25 parts of wolfberry, 6-25 parts of dried tangerine peel, 6-25 parts of mulberry, 6-25 parts of jujube, 6-25 parts of Polygonatum odoratum, 4-15 parts of longan, 4-15 parts of Polygonatum sibiricum and 1-8 parts of donkey-hide gelatin.

[0013] In some embodiments, the composition includes the following raw materials in parts by weight: 15-30 parts of Poria cocos, 10-20 parts of rose, 10-20 parts of wolfberry, 10-20 parts of dried tangerine peel, 10-20 parts of mulberry, 10-20 parts of jujube, 10-20 parts of Polygonatum odoratum, 7-10 parts of longan, 7-10 parts of Polygonatum sibiricum and 3-5 parts of donkey-hide gelatin.

[0014] In some embodiments, the composition includes the following raw materials in parts by weight: 60 parts of Poria cocos, 40 parts of rose, 40 parts of wolfberry, 40 parts of dried tangerine peel, 35 parts of mulberry, 35 parts of jujube, 35 parts of Polygonatum odoratum, 20 parts of longan, 20 parts of Polygonatum sibiricum, and 15 parts of donkey-hide gelatin.

[0015] In some embodiments, the composition includes the following raw materials in parts by weight: 15 parts of Poria cocos, 10 parts of rose, 10 parts of wolfberry, 10 parts of dried tangerine peel, 10 parts of mulberry, 10 parts of jujube, 10 parts of Polygonatum odoratum, 7 parts of longan, 7 parts of Polygonatum sibiricum, and 3 parts of donkey-hide gelatin.

[0016] In some embodiments, the composition includes the following raw materials in parts by weight: 9 parts of Poria cocos, 2 parts of rose, 2 parts of wolfberry, 2 parts of dried tangerine peel, 2 parts of mulberry, 2 parts of jujube, 2 parts of Polygonatum odoratum, 2 parts of longan, 2 parts of Polygonatum sibiricum, and 2 parts of donkey-hide gelatin.

[0017] In some embodiments, the composition includes the following raw materials in parts by weight: 40 parts of Poria cocos, 25 parts of rose, 25 parts of wolfberry, 25 parts of dried tangerine peel, 25 parts of mulberry, 25 parts of jujube, 25 parts of Polygonatum odoratum, 15 parts of longan, 15 parts of Polygonatum sibiricum and 8 parts of donkey-hide gelatin.

[0018] In some embodiments, the composition includes the following raw materials in parts by weight: 30 parts of Poria cocos, 20 parts of rose, 20 parts of wolfberry, 20 parts of dried tangerine peel, 20 parts of mulberry, 20 parts of jujube, 20 parts of Polygonatum odoratum, 10 parts of longan, 10 parts of Polygonatum sibiricum and 5 parts of donkey-hide gelatin.

[0019] In some embodiments, the composition includes the following raw materials in parts by weight: 30 parts of Poria cocos, 30 parts of rose, 30 parts of wolfberry, 30 parts of dried tangerine peel, 30 parts of mulberry, 30 parts of jujube, 30 parts of Polygonatum odoratum, 30 parts of longan, 30 parts of Polygonatum sibiricum, and 10 parts of donkey-hide gelatin.

[0020] In some embodiments, the composition includes the following raw materials in parts by weight: 30 parts of Poria cocos, 30 parts of rose, 30 parts of wolfberry, 30 parts of dried tangerine peel, 30 parts of mulberry, 30 parts of jujube, 30 parts of Polygonatum odoratum, 10 parts of longan, 10 parts of Polygonatum sibiricum, and 5 parts of donkey-hide gelatin.

[0021] In some embodiments, the composition includes the following raw materials in parts by weight: 30 parts of Poria cocos, 30 parts of rose, 30 parts of wolfberry, 30 parts of dried tangerine peel, 30 parts of mulberry, 30 parts of jujube, 30 parts of Polygonatum odoratum, 30 parts of longan, 30 parts of Polygonatum sibiricum, and 15 parts of donkey-hide gelatin.

[0022] The processing and preparation refers to preparing an extract from the ingredients other than donkey-hide gelatin, concentrating the extract and then mixing it with donkey-hide gelatin to prepare the composition. Preferably, rose scented water is collected while preparing the rose extract, and concentrating the extract and then mixing it with the rose scented water and donkey-hide gelatin to prepare the composition.

[0023] In some embodiments, the present invention provides a manufactured product, characterized in that it comprises the above-mentioned composition and acceptable excipients;

[0024] Preferably, the finished product is a medicine, a health food, a food, a health product for external use, an additive or an intermediate;

[0025] Preferably, the finished product is a paste-like food;

[0026] Preferably, the manufactured product is used to increase glucose uptake, enhance ATPase activity, and increase cellular ATP content.

[0027] In some embodiments, the present invention provides a method for preparing the above-mentioned composition, comprising the following steps: 1) extracting the raw materials in several formulas to obtain extracts; 2) combining the extracts and concentrating them into a thick paste; (3) dissolving the donkey-hide gelatin in an appropriate amount of water and mixing it with the thick paste.

[0028] In some embodiments, each raw material in the above step 1 is extracted separately, or 1, 2, 3, 4, 5, 6, 7, or 8 raw materials are optionally extracted separately, and the remaining raw materials are extracted together.

[0029] In some embodiments, in step 1 above, the rose flower and / or polygonatum sibiricum and / or tangerine peel raw materials can be extracted separately or together with other raw materials.

[0030] In some embodiments, rose extract is prepared and rose aromatic water is collected at the same time, and then the rose aromatic water, the donkey-hide gelatin dissolved in water, and the thick paste prepared in step 2) are mixed evenly.

[0031] In some embodiments, the present invention provides a method for preparing the above-mentioned composition, comprising the following steps:

[0032] (1) Extract Poria cocos, rose, wolfberry, tangerine peel, mulberry, jujube, polygonatum, longan, and polygonatum with a solvent to obtain an extract, which is then concentrated into a thick paste; (2) Dissolve donkey-hide gelatin in an appropriate amount of water and mix it with the thick paste.

[0033] In some embodiments, the present invention provides a method for preparing the above-mentioned composition, comprising the following steps:

[0034] (1) soaking Poria cocos, wolfberry fruit, mulberry, jujube, polygonatum, longan, polygonatum sibiricum, and tangerine peel in a solvent, and then extracting to obtain an extract;

[0035] (2) soaking the roses in a solvent and then extracting to obtain an extract; wherein the rose extract is prepared while collecting the rose aromatic water;

[0036] (3) combining the extracts and concentrating them to a thick paste;

[0037] (4) Dissolve the donkey-hide gelatin in an appropriate amount of water and mix it with the thick paste and rose scented water.

[0038] In some embodiments, the present invention provides a method for preparing the above-mentioned composition, comprising the following steps:

[0039] (1) extracting Poria cocos, wolfberry fruit, dried tangerine peel, mulberry, jujube, polygonatum odoratum, and longan with a solvent to obtain an extract;

[0040] (2) extracting the polygonatum with a solvent to obtain an extract;

[0041] (3) extracting the roses with a solvent to obtain an extract, preparing the rose extract and collecting the rose aromatic water;

[0042] (4) combining the extracts and concentrating them to a thick paste;

[0043] (5) Dissolve the donkey-hide gelatin in an appropriate amount of water and mix it with the thick paste and rose scented water.

[0044] In some embodiments, the present invention provides a method for preparing the above-mentioned composition, comprising the following steps:

[0045] (1) extracting Poria cocos, wolfberry fruit, mulberry, jujube, polygonatum, longan, and polygonatum with a solvent to obtain an extract;

[0046] (2) extracting the dried tangerine peel with a solvent to obtain an extract;

[0047] (3) extracting the roses with a solvent to obtain an extract, preparing the rose extract and collecting the rose aromatic water;

[0048] (4) combining the extracts and concentrating them to a thick paste;

[0049] (5) Dissolve the donkey-hide gelatin in an appropriate amount of water and mix it with the thick paste and rose scented water.

[0050] In some embodiments, the preparation method is soaked with a solvent before extraction. Preferably, soaking is only required before the first extraction.

[0051] In some embodiments, the amount of the solvent used for soaking or extraction is 5 to 30 times, 8 to 30 times, 10 to 30 times, 8 to 25 times, 8 to 20 times, or 10 to 15 times.

[0052] In some embodiments, the soaking time is 10 min to 5 h, 10 min to 3 h, 10 min to 2 h, 10 min to 1 h, 15 min to 3 h, 15 min to 1 h, 30 min to 1 h; preferably 1 h, 30 min or 10 min.

[0053] In some embodiments, the extraction is temperature-elevated extraction.

[0054] In some embodiments, the temperature range of the elevated temperature extraction is 45-100°C, preferably 50-90°C, preferably 55-85°C, preferably 60-80°C.

[0055] In some embodiments, the extraction is carried out by water extraction membrane separation, decoction, reflux extraction, microwave extraction, ultrasonic extraction, immersion extraction, dynamic extraction, and / or reduced pressure extraction.

[0056] In some embodiments, the reduced pressure extraction temperature is 50-100°C or 60-80°C.

[0057] In some embodiments, the reduced pressure extraction temperature is 50°C, 60°C, 65°C, 70°C, 80°C, or 90°C.

[0058] In some embodiments, the number of extractions is 1 to 4 times.

[0059] In some embodiments, the number of extractions is 1, 2, 3, or 4 times.

[0060] In some embodiments, the extraction time is 10 min to 5 h, 30 min to 5 h, 30 min to 4 h, 1 h to 4 h, 1.5 to 4 h, 15 min to 3 h, 30 min to 1.5 h or 30 min to 1 h each time.

[0061] In some embodiments, the extraction time is 30 min, 45 min, 1 h, 1.5 h, 2 h, 3 h or 4 h each time.

[0062] In some embodiments, the present invention provides a method for preparing the above-mentioned composition, comprising the following steps: (1) selecting a single or several raw materials in a formula, soaking each of them in 5-30 times the amount of solvent for 10 minutes to 5 hours, and then heating and extracting them 1 to 4 times, each time for 10 minutes to 5 hours, to obtain an extract; (2) combining the extracts and concentrating them into a thick paste; (3) dissolving the donkey-hide gelatin in an appropriate amount of water and mixing it with the thick paste;

[0063] Preferably, the method comprises the following steps: (1) selecting a single or several raw materials in a formula, soaking each of them in 5-30 times the amount of solvent for 15 minutes to 3 hours, and then heating and extracting them 1 to 4 times, each time for 15 minutes to 3 hours, to obtain an extract; (2) combining the extracts and concentrating them into a thick paste; (3) dissolving the donkey-hide gelatin in an appropriate amount of water and mixing it with the thick paste;

[0064] Preferably, the method comprises the following steps: (1) selecting a single or several raw materials in a formula, soaking each of them in 5-30 times the amount of solvent for 10 minutes to 5 hours, and then heating and extracting them 1 to 4 times, each time for 10 minutes to 5 hours, to obtain an extract, wherein the rose extract is prepared while collecting rose aromatic water; (2) combining the extracts and concentrating them into a thick paste; (3) dissolving the donkey-hide gelatin in an appropriate amount of water, and mixing it with the thick paste and rose aromatic water;

[0065] Preferably, the method comprises the following steps: (1) selecting a single or several raw materials in a formula, soaking each of them in 5-30 times the amount of solvent for 15 minutes to 3 hours, and then heating and extracting them 1 to 4 times, each time for 15 minutes to 3 hours, to obtain an extract, wherein the rose extract is prepared and rose aromatic water is collected at the same time; (2) combining the extracts and concentrating them into a thick paste; (3) dissolving the donkey-hide gelatin in an appropriate amount of water and mixing it with the thick paste and rose aromatic water.

[0066] In some embodiments, the extraction is carried out by water extraction membrane separation, decoction, reflux extraction, microwave extraction, ultrasonic extraction, immersion extraction, dynamic extraction, and / or reduced pressure extraction.

[0067] In some embodiments, the solvent is selected from water, ethanol, or methanol. Water is selected from deionized water, pure water, distilled water, and the like.

[0068] In some embodiments, the elevated temperature range is 45-100°C, preferably 50-90°C, preferably 55-85°C, preferably 60-80°C.

[0069] In some embodiments, the raw materials in several formulas are Poria cocos, rose flower, wolfberry fruit, tangerine peel, mulberry, jujube, polygonatum, longan, and polygonatum.

[0070] In some embodiments, the raw materials in several formulas are Poria cocos, wolfberry, mulberry, jujube, polygonatum, longan, polygonatum, and tangerine peel.

[0071] In some embodiments, the raw materials in several formulas are Poria cocos, wolfberry, tangerine peel, mulberry, jujube, polygonatum, and longan.

[0072] In some embodiments, the raw materials in several formulas are Poria cocos, wolfberry, mulberry, jujube, polygonatum, longan, and polygonatum.

[0073] The water extraction membrane separation method involves two steps: water extraction and membrane separation. Water extraction involves placing the medicinal material in a suitable decoction vessel, adding an appropriate proportion of water, bringing it to a boil, maintaining a slight boil for a period of time, and then separating the decoction. Membrane separation involves selectively separating molecules of varying particle sizes through a semipermeable membrane at the molecular level. In some embodiments, membrane separation includes a tubular membrane separation step and an RO membrane separation step.

[0074] The decoction method is to add water to the pre-treated medicinal materials and then heat and boil them to fully extract the effective ingredients in the medicinal materials. This decoction is performed 1-5 times, and the decoctions are collected, precipitated or filtered, and the supernatant is further evaporated and concentrated to a certain concentration.

[0075] Reflux extraction method refers to the use of a condensation reflux device to prevent the loss of solvent volatilization when extracting the active ingredients of traditional Chinese medicine by heating an organic solvent, thereby improving the extraction efficiency.

[0076] Microwave extraction utilizes high-frequency electromagnetic waves to penetrate the extraction medium and reach the interior of the material. During this process, microwave energy is rapidly converted into heat, causing the temperature inside the material's cells to rise rapidly. When the pressure inside the cells exceeds their tolerance, they rupture, allowing the active ingredients to escape and dissolve in the extraction medium at a lower temperature. The extract is then separated through further filtration.

[0077] Ultrasonic extraction utilizes the mechanical, cavitation, and thermal effects of ultrasound to increase the speed of medium molecules and the penetration of the medium to extract effective ingredients.

[0078] The extraction method is a type of extraction. It uses the principle of like dissolves like to soak medicinal materials in liquid solvents to extract the active ingredients in the medicinal materials.

[0079] The reduced pressure extraction method uses the principle of vacuum state and the reduction of solvent boiling point to extract the active ingredients in the medicinal materials in a certain proportion under vacuum environment and appropriate temperature.

[0080] Dynamic extraction involves continuous and thorough contact extraction of medicinal materials and solvents in a leaching vessel. This method extracts natural substances in a gentle, dynamic environment, resulting in lower heating temperatures, less damage to active ingredients, and fewer impurities in the solution. This represents a continuous production process.

[0081] In some embodiments, a method for preparing the above-mentioned finished product is characterized by comprising adding an acceptable excipient to the product of step (3);

[0082] Preferably, the finished product is a medicine, a health food, a food, a health product for external use, an additive or an intermediate;

[0083] Preferably, the finished product is a paste-like food;

[0084] Preferably, the manufactured product is used to increase glucose uptake, enhance ATPase activity, and increase cellular ATP content.

[0085] In some embodiments, the above-mentioned composition or product is used to prepare a product for preventing, treating or assisting in the treatment of increasing glucose uptake, enhancing ATPase activity, and increasing cellular ATP content.

[0086] In some embodiments, the above-mentioned composition or product can delay aging in women.

[0087] In some embodiments, the product is a medicine, a health food, a food, a health product for external use, an additive, or an intermediate;

[0088] In some embodiments, the finished product is a paste-like food.

[0089] The present invention utilizes high-quality medicinal materials that are both medicinal and edible, and can nourish the kidneys and replenish essence, strengthen the spleen and replenish qi, and nourish blood and calm the mind. Based on the mechanisms of aging in modern medicine, the present invention utilizes modern molecular biology techniques and a scientific formula developed using qualitative and quantitative methods. This formula can replenish large amounts of ATP in a short period of time, activate the activity of ATPase, release a large amount of energy, provide energy for cellular activities and metabolism, and delay the onset of aging symptoms. Testing with modern scientific research equipment has found that after consumption, it not only provides high glucose intake and enhances ATPase activity, but also quickly replenishes ATP and provides sufficient energy for the body. DETAILED DESCRIPTION

[0090] The following is a detailed description of the technical solution of the present invention, which does not limit the scope of protection of the present invention. Non-essential modifications and adjustments made by others based on the concept of the present invention still fall within the scope of protection of the present invention.

[0091] As used herein, the term "comprising" means that compositions and methods include the recited elements, but do not exclude others. When used to define compositions and methods, "consisting essentially of" means excluding other elements essential to the composition. For example, as defined herein, a composition consisting essentially of certain elements does not exclude other elements that do not materially affect the basic and novel characteristics of the claimed invention. "Consisting of" means excluding other ingredients and essential method steps beyond the recited amounts. Embodiments defined by each transition clause are within the scope of the present invention.

[0092] As used herein, the singular forms "a," "an," and "the" include plural references unless otherwise specified. Thus, reference to "a composition" also includes the plural "compositions."

[0093] The term "extract" refers to an extract obtained by extracting a raw material using water or an organic solvent.

[0094] The term "combination of extracts" refers to both a mixture of extracts obtained by extracting each raw material separately and an extract obtained by extracting a mixture of raw materials.

[0095] The term "composition" may refer to a single compound or a combination of at least two compounds. For example, a composition may contain an active ingredient extracted from a raw material and acceptable excipients.

[0096] The term "multiple amount" refers to the amount of solvent added (W) as a multiple of the total weight of the raw material.

[0097] "Acceptable excipients" are diluents, adjuvants, vehicles, or vehicles with which the composition is administered. Excipients include any and all physiologically compatible solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like. Examples of excipients include, but are not limited to, sterile liquids such as water, oils, and lipids, such as phospholipids and glycolipids. These sterile liquids include, but are not limited to, those derived from petroleum, animal, vegetable, or synthetic sources, such as peanut oil, soybean oil, mineral oil, sesame oil, and the like.

[0098] "Treating" a patient's disease means preventing the disease from developing in a patient who is susceptible to the disease or who has not yet developed symptoms of the disease; inhibiting the disease or preventing its progression; or alleviating the disease or causing its regression.

[0099] The composition according to the present invention can be used as a nutritional product, a health product, or a pharmaceutical. The term "health food" refers to any product having a nutritional and / or physiological effect, and particularly includes food supplements, foods, dietary products, etc. These products can be administered, in particular, but not limited to, via the oral, gastric, or intravenous routes.

[0100] The raw materials in the disclosed compositions are all commonly used medicinal and edible herbs, which are detailed in the Chinese Pharmacopoeia and Chinese Materia Medica, and are readily available through commercial channels. The present invention does not impose any particular restrictions on the origin of these Chinese medicinal materials, as long as they comply with relevant national standards or regulations.

[0101] Poria (scientific name or correct name), English name Indian Buead Tuckahoe, Latin name Poria.

[0102] Rose (scientific name or correct name), English name Rose, Latin name Rosa Rugosa.

[0103] Wolfberry (scientific name or correct name), English name Babury Wolfberry Fruit, Latin name is Lycii Fructus.

[0104] Tangerine peel (scientific name or correct name), English name Pericarpium Citri Reticulatae, Latin name is Citrus Reticulata Blanco.

[0105] Mulberry (scientific name or correct name), English name Mulberry Fruit, Latin name is Fructus Mori.

[0106] Jujube (scientific name or correct name), English name Jujube, Latin name Ziziphus Jujuba Mill.

[0107] Polygonatum (scientific name or correct name), English name Fragrant Solomonseal Rhizome, Latin name is Rhizoma Polygonati Odorati.

[0108] Longan (scientific name or correct name), English name Dried Longan Pulp, Latin name is Longan Arillus.

[0109] Polygonatum sibiricum (scientific name or correct name), English name Siberia Landpick, Latin name Polygonatum sibiricum Delarb ex Redoute.

[0110] Donkey-hide Gelatin (scientific name or correct name), English name Donkey-hide Gelatin, Latin name Colla Corii Asini.

[0111] Example 1 Extract combination formula and preparation method thereof

[0112] A Chinese herbal medicine extract combination, comprising the following Chinese herbal medicines in parts by weight:

[0113] 60 parts of Poria cocos, 40 parts of Flos Rosae Rugosae, 40 parts of Fructus Lycii, 40 parts of Pericarpium Citri Reticulatae, 35 parts of Mulberries, 35 parts of Jujubes, 35 parts of Polygonatum odoratum, 20 parts of Longans, 20 parts of Polygonatum sibiricum, and 15 parts of Donkey-hide Gelatin.

[0114] (1) Soak Poria cocos, wolfberry, mulberry, jujube, polygonatum, longan, polygonatum sibiricum, and tangerine peel in 25 times water for 30 minutes, and boil for 4 hours to obtain an extract;

[0115] (2) Extract rose flowers once, add 10 times the amount of water to soak for 60 minutes, and extract at 70℃ under reduced pressure for 30 minutes to obtain the extract, and collect the aromatic water at the same time;

[0116] (3) The extracts were combined and concentrated under reduced pressure at 65°C to a thick paste;

[0117] (4) Dissolve the donkey-hide gelatin in an appropriate amount of water, mix it with the thick paste and rose scented water, and adjust it to the appropriate concentration.

[0118] Example 2 Extract combination formula and preparation method thereof

[0119] A Chinese herbal medicine extract combination, comprising the following Chinese herbal medicines in parts by weight:

[0120] 15 parts of Poria cocos, 10 parts of Flos Rosae Rugosae, 10 parts of Fructus Lycii, 10 parts of Pericarpium Citri Reticulatae, 10 parts of Mulberries, 10 parts of Chinese dates, 10 parts of Polygonatum odoratum, 7 parts of Longan, 7 parts of Polygonatum sibiricum, and 3 parts of Donkey-hide Gelatin.

[0121] (1) Soak Poria cocos, wolfberry, mulberry, jujube, polygonatum, longan, polygonatum sibiricum, and tangerine peel in 18 times water for 2 hours, and reflux for 4 hours to obtain an extract;

[0122] (2) Extract rose flowers once, add 15 times the amount of water and soak for 30 minutes, then reduce pressure and extract at 60℃ for 1 hour to obtain the extract and collect the aromatic water;

[0123] (3) Combine the extracts and concentrate under reduced pressure at 60°C to a thick paste;

[0124] (4) Dissolve the donkey-hide gelatin in an appropriate amount of water, mix it with the thick paste and rose scented water, and adjust it to the appropriate concentration.

[0125] Example 3 Extract combination formula and preparation method

[0126] A Chinese herbal medicine extract combination, comprising the following Chinese herbal medicines in parts by weight:

[0127] 9 parts of Poria cocos, 2 parts of rose flowers, 2 parts of wolfberry fruits, 2 parts of dried tangerine peel, 2 parts of mulberries, 2 parts of jujubes, 2 parts of polygonatum, 2 parts of longans, 2 parts of polygonatum, and 2 parts of donkey-hide gelatin.

[0128] (1) Poria cocos, wolfberry, dried tangerine peel, mulberry, jujube, polygonatum, and longan were extracted with water at 60°C for three times by ultrasonic wave, with 8 times the amount of water each time, the first time for 1 hour, and the next two times for 45 minutes each time to obtain the extract;

[0129] (2) Extract Polygonatum sibiricum once, add 10 times the amount of water and soak at 80℃ for 1 hour to obtain the extract;

[0130] (3) Extract rose flowers once, add 15 times the amount of water, and extract at 80°C under reduced pressure for 1 hour to obtain the extract, while collecting the aromatic water;

[0131] (4) The extracts were combined and concentrated under reduced pressure at 70°C to a thick paste;

[0132] (5) Dissolve the donkey-hide gelatin in an appropriate amount of water, mix it with the thick paste and rose scented water, and adjust it to the appropriate concentration.

[0133] Example 4 Extract Combination Formula and Preparation Method

[0134] A Chinese herbal medicine extract combination, comprising the following Chinese herbal medicines in parts by weight:

[0135] 40 parts of Poria cocos, 25 parts of Flos Rosae Rugosae, 25 parts of Fructus Lycii, 25 parts of Pericarpium Citri Reticulatae, 25 parts of Mulberries, 25 parts of Chinese Dates, 25 parts of Polygonatum odoratum, 15 parts of Longan, 15 parts of Polygonatum sibiricum and 8 parts of Donkey-hide Gelatin.

[0136] (1) Poria cocos, rose flower, wolfberry fruit, tangerine peel, mulberry, jujube, polygonatum, longan, and polygonatum are extracted twice under reduced pressure at 80°C, using 12 times the amount of water each time for 1 hour each time to obtain an extract. The obtained extract is concentrated under reduced pressure at 70°C to a thick paste;

[0137] (2) Dissolve the donkey-hide gelatin in an appropriate amount of water, mix it with the thick paste, and adjust it to the appropriate concentration.

[0138] Example 5 Extract Combination Formula and Preparation Method

[0139] A Chinese herbal medicine extract combination, comprising the following Chinese herbal medicines in parts by weight:

[0140] 30 parts of Poria cocos, 20 parts of rose flowers, 20 parts of wolfberry fruits, 20 parts of dried tangerine peels, 20 parts of mulberries, 20 parts of jujubes, 20 parts of polygonatum, 10 parts of longans, 10 parts of polygonatum, and 5 parts of donkey-hide gelatin.

[0141] (1) Soak Poria cocos, wolfberry, mulberry, jujube, polygonatum, longan, and polygonatum in water for 30 minutes, and extract twice at 100°C, with 10 times the amount of water for the first extraction and 8 times the amount of water for the second extraction, each extraction for 1 hour to obtain the extract;

[0142] (2) Extract tangerine peel once, add 15 times water to soak for 60 minutes, and extract at 80℃ for 30 minutes to obtain the extract;

[0143] (3) Extract rose flowers once, add 15 times the amount of water to soak for 60 minutes, and extract at 80℃ under reduced pressure for 30 minutes to obtain the extract, and collect the aromatic water at the same time;

[0144] (4) The extracts were combined and concentrated under reduced pressure at 60°C to a thick paste;

[0145] (5) Dissolve the donkey-hide gelatin in an appropriate amount of water, mix it with the thick paste and rose scented water, and adjust it to the appropriate concentration.

[0146] Example 6 Extract Combination Formula and Preparation Method

[0147] A Chinese herbal medicine extract combination, comprising the following Chinese herbal medicines in parts by weight:

[0148] 30 parts of Poria cocos, 30 parts of rose flowers, 30 parts of wolfberry fruits, 30 parts of dried tangerine peels, 30 parts of mulberries, 30 parts of jujubes, 30 parts of polygonatum, 30 parts of longans, 30 parts of polygonatum, and 10 parts of donkey-hide gelatin.

[0149] (1) Poria cocos, wolfberry, mulberry, jujube, polygonatum, longan, and polygonatum are decocted and extracted twice, the first time with 15 times the amount of water, the second time with 10 times the amount of water, each time for 1 hour to obtain the extract;

[0150] (2) Extract tangerine peel once, add 10 times the amount of water and soak for 60 minutes, then extract at 80℃ for 30 minutes to obtain the extract;

[0151] (3) Extract rose flowers once, add 10 times the amount of water to soak for 60 minutes, and extract at 80℃ under reduced pressure for 30 minutes to obtain the extract, and collect the aromatic water at the same time;

[0152] (4) The extracts were combined and concentrated under reduced pressure at 65°C to a thick paste;

[0153] (5) Dissolve the donkey-hide gelatin in an appropriate amount of water, mix it with the thick paste and rose scented water, and adjust it to the appropriate concentration.

[0154] Example 7 Extract Combination Formula and Preparation Method

[0155] A Chinese herbal medicine extract combination, comprising the following Chinese herbal medicines in parts by weight:

[0156] 30 parts of Poria cocos, 30 parts of rose flowers, 30 parts of wolfberry fruits, 30 parts of dried tangerine peels, 30 parts of mulberries, 30 parts of jujubes, 30 parts of polygonatum, 10 parts of longans, 10 parts of polygonatum, and 5 parts of donkey-hide gelatin.

[0157] (1) Soak Poria cocos, wolfberry, dried tangerine peel, mulberry, jujube, polygonatum, longan, and polygonatum in 20 times the amount of water for 60 minutes and extract at 80°C for 1 hour to obtain the extract;

[0158] (2) Extract rose flowers once, add 10 times the amount of water to soak for 60 minutes, and extract at 70℃ under reduced pressure for 45 minutes to obtain the extract, and collect the aromatic water at the same time;

[0159] (3) The extracts were combined and concentrated under reduced pressure at 55°C to a thick paste;

[0160] (4) Dissolve the donkey-hide gelatin in an appropriate amount of water, mix it with the thick paste and rose scented water, and adjust it to the appropriate concentration.

[0161] Example 8 Extract Combination Formula and Preparation Method

[0162] A Chinese herbal medicine extract combination, comprising the following Chinese herbal medicines in parts by weight:

[0163] 30 parts of Poria cocos, 30 parts of rose flowers, 30 parts of wolfberry fruits, 30 parts of dried tangerine peels, 30 parts of mulberries, 30 parts of jujubes, 30 parts of polygonatum, 30 parts of longans, 30 parts of polygonatum, and 15 parts of donkey-hide gelatin.

[0164] (1) Soak Poria cocos, wolfberry, dried tangerine peel, mulberry, jujube, polygonatum, longan, and polygonatum in 18 times water for 60 minutes, and extract at 80°C under reduced pressure for 1.5 hours to obtain the extract;

[0165] (2) Extract rose flowers once, add 12 times the amount of water to soak for 10 minutes, and extract at 65℃ under reduced pressure for 1 hour to obtain the extract, and collect the aromatic water at the same time;

[0166] (3) Combine the extracts and concentrate under reduced pressure at 65°C to a thick paste;

[0167] (4) Dissolve the donkey-hide gelatin in an appropriate amount of water, mix it with the thick paste and rose scented water, and adjust it to the appropriate concentration.

[0168] Effect test

[0169] 1. Experimental model:

[0170] Rat cardiomyocyte cell line (H9c2, American Type Culture Collection), human hepatoma cell line (HepG2, American Type Culture Collection), and mouse myoblast cell line (C2C12, American Type Culture Collection)

[0171] 2. Experimental reagents:

[0172] DMEM high-glucose medium (Dulbecco's modified eagle medium, Invitrogen, Carlsbad, CA), fetal bovine serum (Fetal bovine serum, Invitrogen, Carlsbad, CA), bovine serum albumin (Bovine serum albumin, USB, Cleveland, Ohio, USA), Trypsin-EDTA (Trypsin-EDTA, Invitrogen, Carlsbad, CA), and a glucose test kit (Beijing Pulilai).

[0173] 3. Experimental instruments:

[0174] Microplate Reader(Dynex Opsys MR TM ), Victor V3 Multi-label Counter (Perkin Elmer, Turku, Finland)

[0175] 4. Experiment:

[0176] (1) Cell culture: After H9c2 cells, HepG2 cells, and C2C12 cells were revived, they were transferred to culture dishes using DMEM high-glucose medium containing 10% fetal bovine serum and cultured in a constant-temperature incubator at 37°C and 5% CO2. After the cells adhered to the wall and grew confluently, they were digested with trypsin. H9c2 cells were passaged once every three days at a ratio of 1:4, HepG2 cells were passaged once every three days at a ratio of 1:4, and C2C12 cells were passaged once every three days at a ratio of 1:10. Cells in the logarithmic growth phase were used for experiments.

[0177] (2) In situ ATP generation assay (ATP-GC in situ): H9c2 cells in the logarithmic growth phase were seeded at a density of 25,000 cells / well in a 24-well cell culture plate. A normal cell control group and a test sample treatment group were set up separately. After the cells were stably attached to the plate for 24 hours, freshly prepared cell culture medium containing 3 and 10 mg / mL of the test sample was added and placed in a constant temperature incubator at 37°C and 5% CO2 for a certain period of time. The amount of ATP in situ and the amount of ATP generated within 0-15 minutes were measured by bioluminescence assay, and the untreated cells were used as the control group for normalization. The area under the curve (AUC) was calculated.

[0178] Table 1 shows the changes in intracellular ATP levels within 15 minutes after H9c2 cells were stimulated by the composition for 2, 6 and 24 hours, respectively.

[0179] Table 1. Effects of the test products on ATP-GC in H9c2 cells

[0180] Control AUC2 value=750.0±13.2; *: p<0.05.

[0181] The experimental results showed that compared with the normal control group, the 10 mg / mL composition significantly increased the production of ATP by about 30% after 24 hours of action, increased cell energy, and was beneficial to the production of "qi" in the human body.

[0182] (3) In situ antioxidant capacity assay (GSH): H9c2 cells were treated with the drug for a specific period of time and then seeded into 12-well cell culture plates at a density of 37,500 cells / well. A normal cell control group and a test sample treatment group were set up. After the cells were allowed to grow stably on the plate for 24 hours, freshly prepared cell culture medium containing 10 mg / mL of the test sample was added and the cells were incubated in a constant temperature incubator at 37°C and 5% CO2 for a specific period of time. Intracellular GSH levels were measured using the Griffith method.

[0183] The experimental results showed that GSH in H9c2 cells increased by about 20% (p=0.005) after 24 hours of stimulation with the drug (10 mg / mL), indicating that the Chinese medicine composition can significantly improve the antioxidant capacity of cells.

[0184] (4) ATP level determination: HepG2 cells in the logarithmic growth phase were seeded at a density of 5000 cells / well in 96-well cell culture plates. A normal cell control group and a test sample treatment group were set up. After the cells were allowed to grow stably on the plate for 24 hours, freshly prepared cell culture medium containing 0.3 and 1 mg / mL of the test sample was added and incubated in a 37°C, 5% CO2 incubator for 2 and 6 hours, respectively.

[0185] Table 2 shows the changes in intracellular ATP levels after the composition stimulated HepG2 cells for 2 and 6 hours, respectively.

[0186] Table 3 shows the changes in intracellular ATP hydrolase activity after the composition stimulated HepG2 cells for 2 and 6 hours, respectively.

[0187] Table 2. Effects of the test articles on ATP in HepG2 cells (2 and 6 hours)

[0188] Table 3. Effects of the test articles on ATP hydrolase activity of HepG2 cells (2 and 6 hours)

[0189] The experimental results showed that compared with the normal control group, stimulating HepG2 cells for 6 hours with 0.3 and 1 mg / mL of the extract increased ATP production by approximately 25% and 30% respectively. Furthermore, the extract significantly reduced the activity of hydrolases in HepG2 cells, indicating that the composition can promote ATP production in HepG2 cells and inhibit ATP hydrolysis in HepG2 cells.

[0190] (5) C2C12: C2C12 cells in the logarithmic growth phase were seeded at a density of 8,000 cells / well in 96-well cell culture plates. A normal cell control group and a test sample-treated group were set up. After the cells were allowed to grow stably on the plate for 24 hours, freshly prepared cell culture medium containing 0.3 and 1 mg / mL of the test sample was added and incubated in a 37°C, 5% CO2 incubator for 4 and 6 hours, respectively.

[0191] Table 4 shows the changes in intracellular ATP levels after the composition stimulated C2C12 cells for 4 and 6 hours, respectively.

[0192] Table 4. Effects of the test articles on ATP in C2C12 cells (4 and 6 hours)

[0193] The experimental results showed that compared with the normal control group, 0.3 and 1 mg / mL of the extract stimulated C2C12 cells for 6 hours and increased ATP production in both cells by approximately 10% and 27%, respectively, indicating that the Chinese herbal composition can promote ATP production in C2C12 cells.

[0194] (6) Determination of glucose uptake: HepG2 cells and C2C12 cells in the logarithmic growth phase were seeded in 96-well cell culture plates at a cell density of 5,000 cells / well and 8,000 cells / well, respectively. The cells were divided into a blank (no cell) group, a normal cell control group, and a test sample-treated group. Each group had three replicate wells and was placed in a constant temperature incubator at 37°C and 5% CO2. After 24 hours of drug addition, the cell culture medium was removed to determine the glucose content and calculate the cellular glucose uptake. The glucose content in the cell culture supernatant was determined using the glucose oxidase method (glucose test kit), and the glucose content in the cell culture medium was subtracted from the mean glucose content of the blank group without inoculated cells to obtain the glucose uptake of the cells in each well.

[0195] Table 5 shows the changes in cellular glucose uptake levels after the composition stimulated C2C12 cells for 2, 4 and 6 hours, respectively.

[0196] Table 6 shows the changes in cellular glucose uptake levels after HepG2 cells were stimulated by the composition for 2, 4 and 6 hours, respectively.

[0197] Table 5. Effects of the test articles on glucose uptake in C2C12 cells (2, 4 and 6 hours)

[0198] Table 6. Effects of the test articles on glucose uptake in HepG2 cells (2, 4 and 6 hours)

[0199] The experimental results show that in the glucose uptake test, the combination treatment can promote the glucose uptake level of C2C12 cells and HepG2 cells, improve the cells' sugar absorption ability, and promote cell metabolism to produce more energy.

[0200] Note: The test sample added to the experimental sample treatment group was prepared according to Example 5.

[0201] Statistical methods: SPSS 22.0 was used for statistical analysis. The level of statistical significance was set at P ≤ 0.05. The measurement data were expressed as mean ± standard deviation. One-way analysis of variance (ANOVA) test was used for statistical analysis. Statistical differences and biological significance were considered in the evaluation.

Claims

1. A composition, characterized in that The composition comprises the following raw materials: tuckahoe, rose, wolfberry, tangerine peel, mulberry, jujube, polygonatum, longan, polygonatum and donkey-hide gelatin; the composition is prepared by processing the raw materials.

2. The composition according to claim 1, wherein The composition comprises the following raw materials in parts by weight: 1-60 parts of Poria cocos, 1-40 parts of rose flowers, 1-40 parts of wolfberry, 1-40 parts of dried tangerine peel, 1-40 parts of mulberry, 1-40 parts of jujube, 1-40 parts of polygonatum, 1-30 parts of longan, 1-30 parts of polygonatum and 1-20 parts of donkey-hide gelatin; Preferably, the composition comprises the following raw materials in parts by weight: 1-60 parts of Poria cocos, 1-40 parts of rose flowers, 1-40 parts of wolfberry, 1-40 parts of dried tangerine peel, 1-40 parts of mulberry, 1-40 parts of jujube, 1-40 parts of polygonatum, 1-20 parts of longan, 1-20 parts of polygonatum and 1-10 parts of donkey-hide gelatin; Preferably, the composition comprises the following raw materials in parts by weight: 3-60 parts of Poria cocos, 2-40 parts of rose flowers, 2-40 parts of wolfberry, 2-40 parts of dried tangerine peel, 2-40 parts of mulberry, 2-40 parts of jujube, 2-40 parts of polygonatum, 2-20 parts of longan, 2-20 parts of polygonatum and 1-10 parts of donkey-hide gelatin; Preferably, the composition comprises the following raw materials in parts by weight: 9-40 parts of Poria cocos, 6-25 parts of rose flowers, 6-25 parts of wolfberry, 6-25 parts of dried tangerine peel, 6-25 parts of mulberry, 6-25 parts of jujube, 6-25 parts of polygonatum, 4-15 parts of longan, 4-15 parts of polygonatum and 1-8 parts of donkey-hide gelatin; Preferably, the composition comprises the following raw materials in parts by weight: 15-30 parts of Poria cocos, 10-20 parts of rose flowers, 10-20 parts of wolfberry, 10-20 parts of dried tangerine peel, 10-20 parts of mulberry, 10-20 parts of jujube, 10-20 parts of polygonatum, 7-10 parts of longan, 7-10 parts of polygonatum and 3-5 parts of donkey-hide gelatin; Preferably, the composition comprises the following raw materials in parts by weight: 60 parts of Poria cocos, 40 parts of rose flowers, 40 parts of wolfberry, 40 parts of dried tangerine peel, 35 parts of mulberry, 35 parts of jujube, 35 parts of Polygonatum odoratum, 20 parts of longan, 20 parts of polygonatum, and 15 parts of donkey-hide gelatin; Preferably, the composition comprises the following raw materials in parts by weight: 15 parts of Poria cocos, 10 parts of rose flowers, 10 parts of wolfberry, 10 parts of dried tangerine peel, 10 parts of mulberry, 10 parts of jujube, 10 parts of polygonatum, 7 parts of longan, 7 parts of polygonatum, and 3 parts of donkey-hide gelatin; Preferably, the composition comprises the following raw materials in parts by weight: 9 parts of Poria cocos, 2 parts of rose flowers, 2 parts of wolfberry, 2 parts of dried tangerine peel, 2 parts of mulberry, 2 parts of jujube, 2 parts of polygonatum, 2 parts of longan, 2 parts of polygonatum, and 2 parts of donkey-hide gelatin; Preferably, the composition comprises the following raw materials in parts by weight: 40 parts of Poria cocos, 25 parts of rose flowers, 25 parts of wolfberry, 25 parts of dried tangerine peel, 25 parts of mulberry, 25 parts of jujube, 25 parts of polygonatum, 15 parts of longan, 15 parts of polygonatum and 8 parts of donkey-hide gelatin; Preferably, the composition comprises the following raw materials in parts by weight: 30 parts of Poria cocos, 20 parts of rose flowers, 20 parts of wolfberry, 20 parts of dried tangerine peel, 20 parts of mulberry, 20 parts of jujube, 20 parts of polygonatum, 10 parts of longan, 10 parts of polygonatum and 5 parts of donkey-hide gelatin; Preferably, the composition comprises the following raw materials in parts by weight: 30 parts of Poria cocos, 30 parts of rose flowers, 30 parts of wolfberry, 30 parts of dried tangerine peel, 30 parts of mulberry, 30 parts of jujube, 30 parts of polygonatum, 30 parts of longan, 30 parts of polygonatum, and 10 parts of donkey-hide gelatin; Preferably, the composition comprises the following raw materials in parts by weight: 30 parts of Poria cocos, 30 parts of rose flowers, 30 parts of wolfberry, 30 parts of dried tangerine peel, 30 parts of mulberry, 30 parts of jujube, 30 parts of Polygonatum odoratum, 10 parts of longan, 10 parts of Polygonatum sibiricum, and 5 parts of donkey-hide gelatin; Preferably, the composition comprises the following raw materials in parts by weight: 30 parts of Poria cocos, 30 parts of rose, 30 parts of wolfberry, 30 parts of dried tangerine peel, 30 parts of mulberry, 30 parts of jujube, 30 parts of Polygonatum odoratum, 30 parts of longan, 30 parts of Polygonatum sibiricum, and 15 parts of donkey-hide gelatin.

3. A finished product, characterized in that: Comprising the composition according to any one of claims 1 to 2 and acceptable excipients; Preferably, the finished product is a medicine, a health food, a food, a health product for external use, an additive or an intermediate; Preferably, the finished product is a paste-like food; Preferably, the manufactured product is used to increase glucose uptake, enhance ATPase activity, and increase cellular ATP content.

4. A method for preparing the composition according to any one of claims 1 to 2, comprising the following steps: (1) Select one or more raw materials from the formula, soak them in 5-30 times the amount of solvent for 10 minutes to 5 hours, then heat and extract them 1-4 times, each time for 10 minutes to 5 hours, to obtain an extract; (2) Combine the extracts and concentrate them into a thick paste; (3) Dissolve the donkey-hide gelatin in an appropriate amount of water and mix it with the thick paste; Preferably, the method comprises the following steps: (1) selecting a single or several raw materials in a formula, soaking each of them in 5-30 times the amount of solvent for 15 minutes to 3 hours, and then heating and extracting them 1 to 4 times, each time for 15 minutes to 3 hours, to obtain an extract; (2) combining the extracts and concentrating them into a thick paste; (3) dissolving the donkey-hide gelatin in an appropriate amount of water and mixing the mixture with the thick paste; Preferably, the method comprises the following steps: (1) selecting a single or several raw materials in a formula, soaking each of them in 5-30 times the amount of solvent for 10 minutes to 5 hours, and then heating and extracting them 1 to 4 times, each time for 10 minutes to 5 hours, to obtain an extract, wherein the rose extract is prepared while collecting rose aromatic water; (2) combining the extracts and concentrating them into a thick paste; (3) dissolving the donkey-hide gelatin in an appropriate amount of water, and mixing it with the thick paste and rose aromatic water; Preferably, the method comprises the following steps: (1) selecting a single or several raw materials in a formula, soaking each of them in 5-30 times the amount of solvent for 15 minutes to 3 hours, and then heating and extracting them 1 to 4 times, each time for 15 minutes to 3 hours, to obtain an extract, wherein the rose extract is prepared and rose aromatic water is collected at the same time; (2) combining the extracts and concentrating them into a thick paste; (3) dissolving the donkey-hide gelatin in an appropriate amount of water and mixing it with the thick paste and rose aromatic water.

5. The method for preparing the composition according to claim 4, wherein: The extraction method includes water extraction membrane separation, decoction, reflux extraction, microwave extraction, ultrasonic extraction, leaching, dynamic extraction, and / or reduced pressure extraction; Preferably, the solvent is selected from: water, ethanol or methanol; Preferably, the temperature range of the heating is 45-100°C, preferably 50-90°C, preferably 55-85°C, preferably 60-80°C.

6. A method for preparing the finished product according to claim 3, characterized in that: The method comprises adding an acceptable auxiliary material to the product prepared according to claim 4 or 5 to prepare the finished product; Preferably, the finished product is a medicine, a health food, a food, a health product for external use, an additive or an intermediate; Preferably, the finished product is a paste-like food; Preferably, the manufactured product is used to increase glucose uptake, enhance ATPase activity, and increase cellular ATP content.

7. Use of the composition according to any one of claims 1 to 2 or the product according to claim 3 in the preparation of a product for preventing, treating or assisting in the treatment of increased glucose uptake, enhanced ATPase activity, or increased cellular ATP content.

8. Use according to claim 7, characterized in that The product can delay female aging.

9. The use according to claim 7 or 8, characterized in that The product is a medicine, health food, food, external health product, additive or intermediate.

10. The use according to any one of claims 7 to 9, characterized in that The finished product is a paste-like food.

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