Automatic virus culture and harvest device by modified peristaltic pump technology

Abstract

In this invention, by changing the operation system of the peristaltic pump in the virus culturing and harvesting device, the service life of the related equipment is prolonged and the maintenance costs are reduced. To this end, in the center of the pump, several metal cylinders with polished surfaces are fixed around each other by a piece of circular gaskets. A ball bearing is embedded at the place where the cylinders are connected to the gaskets, which makes the cylinder rotate easily.

Description

DescriptionTitle of Invention : Automatic virus culture and harvest device by modified peristaltic pump technologyTechnical Field

[0001] The technical field of this invention relates to the virus culture and harvest devices by in ovo vaccination method which are used for vaccine production.Background Art

[0002] Automated egg injection machine and method with application number10868361 is filed in the USPTO.

[0003] This invention is an egg injection device with pneumatic operation, which includes a frame with a pair of parallel tracks in a line through an injection section and a transfer section in series. A parallel track injection assembly consists of multiple injectors fixed by a holding plate to simultaneously inject the virus into the same area.

[0004] Fluid delivery systems adjust the dose of the virus to be injected into the egg through an injection needle by increasing and decreasing pressure, friction, and time. A transfer assembly consists of multiple transfer suckers that lift the injected egg by creating reverse pressure in an annulus around the injection hole while maintaining the injection hole at atmospheric pressure. Therefore, they prevent negative pressure inside the egg.

[0005] As shown in the figures of this invention, this invention does not disclose the function of the virus delivery system into the egg. The claimed invention provides a new system for delivering the virus into the egg with high accuracy.Technical Problem

[0006] In the devices for culturing and harvesting the virus through in-ovo vaccination, first, a small hole is made in the upper surface of the egg, and subsequently, a certain amount of virus is injected into the egg and the hole is covered.

[0007] Since the amount of liquid virus injected into the egg must be very accurate, the peristaltic pumps are used. However, the peristaltic pumps in the in-ovovaccination culture and harvest devices need to replace the special silicone hoses after every 4-5 injections.

[0008] The reason behind this is that during the suction of the fluid (virus fluid or washing fluid), the silicone hose creates friction with the pump rollers, causing the hose to heat up, and as a result, the service life of the hose is reduced. This friction occurs because the outer layer of the pump roller is made of polymer.

[0009] In this invention, by changing the material of the pump roller and the way the roller collides with the hose, peristaltic pumps whose hose service life is several times that of the existing, and also have the necessary precision for the amount of fluid injection have been designed.Solution to Problem

[0010] In this invention, by changing the operation system of the peristaltic pump in the virus culturing and harvesting device, the service life of the related equipment is prolonged and the maintenance costs are reduced. To this end, in the center of the pump, several metal cylinders with polished surfaces are fixed around each other by a piece of circular gaskets. A ball bearing is embedded at the place where the cylinders are connected to the gaskets, which makes the cylinder rotate easily.

[0011] The gaskets are placed inside the holding piece, which, of course, allows the gaskets to rotate inside the holding piece. The shaft is embedded in the middle of the two pieces of gaskets. As the shaft rotates, the gaskets move, and as a result, the cylinders also rotate around the circular axis.

[0012] The fluid transfer hose (which in this invention the fluid is virus or detergent flows through the hose), is placed on the upper surface of the cylinders with an angle of 90sto the cylinders. As the shaft rotates the gasket, the cylinders make contact with the hose.

[0013] Simultaneous pressure and tension of the cylinder on the surface of the hose create suction in the hose and can make the fluid flow inside the hose. While the cylinders revolve by the shaft and gasket, the collision of the cylinders with the hose leads to the rotation of the cylinders. The rotation of each cylinder alsoprevents friction between the cylinder and the hose, and on the other hand, the suction and transfer of fluid inside the hose are completely done.

[0014] In this way, when the needle is placed inside the egg, the shaft moves to the extent defined for the device system, and when the cylinders collide with the hoses, suction is created, and the virus or any other fluid flows. This method also provides the precision needed to control the volume of fluid delivered into the egg. A cartridge is used to keep the hose fixed on the cylinder.

[0015] The top and bottom of the hose are fixed at the inlet and outlet of the cartridge. After entering the cartridge, the hose goes up and passes over the cylinders in a semicircle manner and exits from the other side of the cartridge. In this way, the hose is placed on the upper half of the cylinder circle. In the device, the number of cartridges are placed next to each other so that by rotating the cylinders, the suction occurs simultaneously in the hoses, and injection is carried out on the other side.

[0016] In this way, when the needle is placed inside the egg, the shaft moves to the extent defined for the device system, and when the cylinders collide with the hoses, suction is created, and the virus or any other fluid flows. This method also provides the precision needed to control the volume of fluid delivered into the egg.Advantageous Effects of Invention

[0017] 1 . Obviating the need for replacement of the hose

[0018] 2. High accuracy of fluid injection rate through each hose

[0019] 3. Simplification of the injection system and thus easier maintenance

[0020] 4. The easier replacement of hosesBrief Description of Drawings

[0021] Fig 1 : Schematic of the device

[0022] Fig 2: Peristaltic pump parts

[0023] Fig 3: Peristaltic pump cartridge

[0024] Fig 4: The cylinder's connections to the holding pieceDescription of Embodiments

[0025] Peristaltic pumps (1) are installed in the upper part of the automatic virus culturing and harvesting device. The fluid is sucked from the tanks by the hose (101 ) to the pumps and moves from the other side of the pump to the injection system through the hose (102). The metal cylinders (103) in the center of the pump are fixed around each other by the circular gasket (108).

[0026] On each side of the cylinder there is a ball bearing (109) that connects the cylinder to the holding piece (104). A shaft (108) passes through the center of the gasket and rotates the gasket when the shaft is rotated by the machine's motor. As the gaskets rotate, the cylinders are pulled on the hose and create suction.

[0027] The hose is incorporated inside the cartridge (105), on the cylinders. The top and bottom of the hose at the inlet and outlet of the cartridge are fixed by a fastener (106). After entering the cartridge, the hose goes up in a semi-circle manner (107), passes over the cylinders, and exits from the other side of the cartridge.Examples

[0028] To utilize this pump, following the installation of the pump in the device, it is enough to pass the fluid delivery hose through the cartridge and connect it to the relevant parts. Since the surface of the cylinders is completely polished, it does not damage them in contact with the hose and increases the service life of the hoses. At the same time, the adjustable rotation of the cylinders can bring the necessary precision in the amount of fluid injection.

Claims

Claims

1. A peristaltic pump for fluid delivery through a hose, which comprising: a. Metal cylinders b. Holding piece c. Cartridge d. gasket e. Fluid delivery hoseWherein, the fluid delivery hose passes through the cartridge and is fixed on top of the cylinders, While the cylinders revolve by the shaft and gasket in the holding piece, the collision of the cylinders with the hose leads to the rotation of the cylinders, and suction is created.

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