A disinfectant composition for non-porous surfaces

Abstract

Provided is a disinfectant composition for reducing microbial contaminants on direct and indirect food contact surfaces. In particular, the composition demonstrates a synergistic disinfection mechanism by combining the diffusion mechanism of a booster additive and a peroxy acid. Also provided is a method of reducing microbial contaminants of a surface of a substrate through the deposition of the composition onto a substrate. Finally, provided is a method of disinfecting bottles in cold aseptic filling applications.

Description

A DISINFECTANT COMPOSITION FOR NON-POROUS SURFACESTECHNICAL FIELD

[0001] Provided is a disinfectant composition for reducing microbial contaminants on direct and indirect food contact surfaces. In particular', the composition demonstrates a synergistic disinfection mechanism by combining the diffusion mechanism of a booster agent or additive and a peroxy acid. Also provided is a method of reducing microbial contaminants of a surface through the treatment, application, or deposition of the composition onto a substrate. Finally, provided is a method of using the composition for disinfecting substrates, for example those used in cold aseptic filling (CAF) applications.BACKGROUND

[0002] Food processing companies produce various beverages and dairy products. Food safety holds a very high importance and hence companies deploy various steps of cleaning, sanitization and disinfection. During processing the pathogen associated with food or process equipment must be killed and packed in sterilized primary containers.

[0003] Peracetic acid (PAA) and peroxycarboxylic acids are known for their use as antimicrobial agents. However, conventional peroxycarboxylic acids have inherent disadvantages of limited storage stability, and water solubility. In addition, peroxycarboxylic acids compositions are not effective if low concentrated and / or used at lower temperatures.

[0004] In addition, there are regulatory concerns to improve food safety, the processors use high concentration of chemicals like acetic acid or chlorine source. This often leads to concern on operator safety, compatibility issues with equipment and high cost.

[0005] Food processors prefer to produce through cold aseptic filling techniques which have minimum manual intervention have the advantage of long shelf life, keeping original flavor and nutrient of the products, saving packaging material cost compared to the conventional hot fill which can be sensitive to most of the plant based milk, cereal drinks and fruits juices.

[0006] Cold-aseptic filling (CAF) is now a widely used process that is consistently being adapted to new demands. Cold aseptic filling (CAF) involves bottling a product at ambient or even lower temperatures. Aseptic filling is recommended for beverages such as fruit juices, tea beverages, sports drinks, vegetable juices, milk -based mixed drinks, ultra-heat treated (UHT) milk, near-water drinks, and flavored waters.

[0007] The primary food packaging is a one way plastic bottle which is disinfected and rinsed through cold aseptic filling (CAF) technology. A typical CAF line processing starts from polyethylene terephthalate (PET) bottles being fed from the blow - molding machine or bottle stored into the aseptic area by air conveyors using sterile air, and passed via an airlock inside the clean room. From this point the bottles are handled by the neck only and is an aseptic area. Bottles are first sterilized in an injector, which is usually enclosed in a class 1000 environment. The bottles are passed to a rinser, where destroyed microorganisms if any are removed. Here, the inverted bottles are submitted to a number of intermittent rinses with sterile air and sterile water, which also removes any traces of disinfectant. The bottleneck is rinsed with sterile water before filling. After rinsing, the bottles are filled in controlled clean conditions and closed with a sterilized cap before re - emerging into the normal environment of the bottling hall via a second airlock. The rinser, filler, and capper units are usually housed in a class 100 clean room cabinet. Operators only get access to the class 100 area by using gloves that are fastened to the enclosure. In 100 level clean, the maximum allowable number of dust particles per cubic meter (greater or equal to 0.5micrometer) is 3500, and the number of planktonic bacteria is 5.

[0008] Peracetic acid (PAA) and peroxide containing compositions are used along with surfactant to improve wetting on the surface and meet the required disinfection / sterilization levels. No unsterility in 100000 bottles is one of the targets. However, peroxide containing compositions are not storage stable and the compositions have a tendency of phase separation, which has the disadvantage of a dramatically decreased antimicrobial effect.

[0009] While the disinfection concentration of PAA (peracetic acid) prescribed by OEMs (Original Equipment Manufacturers) is 2000-3000 ppm as per customer input, practical range is 2000-4000ppm (along with 800-1000 ppm surfactant) for primary packaging disinfection; there are also the associated threshold limits of hydrogen peroxide in the bath / tank. As the CAF processing lines continue using recommended levels of PAA, due tothe reversible reaction of acetic acid, peracetic acid, and hydrogen peroxide, the concentration of hydrogen peroxide keeps building up in the disinfection solution bath. It is known that, as per recommendation of many OEMs, the critical threshold of hydrogen peroxide concentration is 10000-15000 ppm, where the disinfectant solution needs to be partially or completely discarded and replaced to reduce the H2O2 concentration in the disinfectant solution. This is observed to be a major drawback of the processors and conventional solutions, as it leads to very high chemical consumption. In addition, with in- use of PAA concentrations of 2000-3000 ppm because of concern of equipment corrosion due to higher atmospheric exposure of PAA.

[0010] Some efforts with minimal success were attempted, such as, commonly used PAA grades being replaced by lower hydrogen peroxide PAA grades along with different surfactant packages. Commonly used PAA grades have the ratio of peracetic acid:hydrogen peroxide as 15:23 (w / w) while lower hydrogen peroxide grades have ratios of peracetic acid and hydrogen peroxide. 15:10 (w / w).

[0011] Many of the current processes and products are focused on reducing hydrogen peroxide content of the PAA (peracetic acid solution) or using different derivatives of peroxy acids.

[0012] The proposed solution focuses on reducing the usage of PAA with use of a synergy booster agent or formulation, which ultimately will reduce the hydrogen peroxide content as well as other benefits, such as, providing improved disinfection properties at significantly lower PAA levels compared to conventional solutions leading to a reduction in hydrogen peroxide buildup. The reduction in PAA will also reduce equipment corrosion and operator safety. There continues to be a need to lower consumption of PAA usage, which results in delayed buildup of hydrogen peroxide.

[0013] Additional objects, advantages, and features of what is claimed will be set forth in the description that follows and in part will become apparent to those skilled in the art upon examination of the following or may be learned by the practice of the technology. The objects and advantages of the presently disclosed and claimed inventive concepts will be realized and attained by means of the compositions and methods particularly pointed out in the appended claims, including the functional equivalents thereof.BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The present disclosure will hereinafter be described in conjunction with the following drawing figures, wherein like numerals denote like elements, and wherein:

[0015] FIG. 1, depicts a PET Aseptic Filling Line: Process Flow Chart.SUMMARY

[0016] This summary is provided to introduce a selection of concepts in a simplified form further described below. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used as an aid in determining the scope of the claimed subject matter.

[0017] Provided is a composition comprising a peroxy acid comprising a C1-22 carboxylic acid; and a booster agent or formulation comprising one or more alpha or beta hydroxy carboxylic acids different from the peroxy acid, one or more alkyl polyglucosides, one or more fatty alcohol alkoxylatcs, one or more acidulating agents.

[0018] The term booster agent or formulation is used interchangeably throughout the application and refers to the formulation that includes one or more alpha or beta hydroxy carboxylic acids different from the peroxy acid, one or more alkyl polyglucosides, one or more fatty alcohol alkoxylates, for example an alkyl capped alcohol alkoxylate, one or more acidulating agents, and optionally one or more C1-12 carboxylic acids different from the peroxy acid and alpha or beta hydroxy carboxylic acids, which is then added or combined with the peroxy acid.

[0019] Also provided is a method of reducing microbial contaminants on a surface of a substrate. The process includes treating the surface of the substrate with a composition that includes a peroxy acid comprising a Ci-22 carboxylic acid, which is combined with a booster agent or formulation comprising one or more alpha or beta hydroxy carboxylic acids different from the peroxy acid, one or more alkyl polyglucosides, one or more fatty alcohol alkoxylates, one or more acidulating agents, and optionally one or more C1-12 carboxylic acids different from the peroxy acid,.

[0020] Finally, provided is a method of disinfecting bottles in cold aseptic filling machineswherein at least one surface of a bottle is treated with a composition that includes a peroxy acid comprising a C1-22 carboxylic acid, which is combined with a booster agent or formulation that includes one or more alpha or beta hydroxy carboxylic acids, one or more alkyl polyglucosides, one or more fatty alcohol alkoxylates, at least one acidulating, and optionally one or more C1-12 carboxylic acids different from the peroxy acid and alpha or beta hydroxy carboxylic acids,.DETAILED DESCRIPTION

[0021] The following detailed description is merely exemplary in nature and is not intended to limit the invention or the application and uses of the invention. As used herein, the word "exemplary" means "serving as an example, instance, or illustration." Thus, any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments. All the embodiments described herein are exemplary embodiments provided to enable persons skilled in the art to make or use the invention and not to limit the scope of the invention which is defined by the claims.

[0022] Furthermore, there is no intention to be bound by any expressed or implied theory presented in the preceding technical field, background, summary, or the following detailed description.

[0023] Unless specifically stated or obvious from context, as used herein, the term "about" is understood as within a range of normal tolerance in the art, for example within 2 standard deviations of the mean. "About" can be understood as within 10%, 5%, 1%, 0.5%, 0.1%, 0.05%, or 0.01% of the stated value. "About" can alternatively be understood as implying the exact value stated. Unless otherwise clear from the context, all numerical values provided herein are modified by the term "about."

[0024] Provided is a composition comprising that includes a Ci-22 peroxy acid; and a booster agent or formulation that includes one or more alpha or beta hydroxy carboxylic acids, one or more alkyl polyglucosides, one or more fatty alcohol alkoxylates, at least one acidulating agent, and optionally one or more C1-12 carboxylic acid different from the peroxy acid and alpha or beta hydroxy carboxylic acids,.

[0025] Through extensive studies it was found that microbial efficacy was improved when abooster agent or formulation containing one or more C1-12 carboxylic acids different from the peroxy acid, one or more alpha or beta hydroxy carboxylic acids different from the peroxy acid; one or more alkyl polyglucosides; one or more fatty alcohol alkoxylates, for example, alkyl capped alcohol alkoxylates; and at least one acidulating agent was added to peracetic acid. This resulted in significant reductions in hydrogen peroxide build-up during the disinfection of bottles.

[0026] The Lower buildup of hydrogen peroxide allows the same disinfectant solution to be used for a longer duration which can be as long as three times than current practice. This controls the concentration of hydrogen peroxide below the critical-threshold safety level for longer periods of time. The composition was also found to improve the efficiency of disinfecting since it requires less process stoppage, reduce water consumption, as well as lower labor costs.

[0027] In some aspects of the method, the weight ratio of the booster agent to peroxy acid is from about 0.01:1 to about 50:1, or from about 0.01:1 to about 15:1, or from about 0.5:1 to about 2:1.

[0028] In some aspects of the composition, the peroxy acid can be chosen from peracetic acid, performic acid, peroxyhexanoic acid or caproic acid, peroxyheptanoic acid, peroxyoctanoic acid or percaprylic acid, peroxynonanoic acid, peroxydecanoic acid or percapric acid, peroxyundecanoic acid, peroxydodecanoic acid or periauric acid, perglycolic acid, peroxy ascorbic acid, peroxy lactic acid, peroxy adipic acid, peroxy citric acid, peroxypimelic acid, peroxysuberic acid, and combinations thereof.

[0029] In some aspects of the composition the peroxy acid comprises from about 0.05 wt.% to about 0.20 wt.%, or from about 0.1 wt.% to about 0.15 wt.% of the total composition.

[0030] In some aspects of the composition, the alpha hydroxy acid of the booster agent can be chosen from lactic acid, glycolic acid, citric acid, malic acid, mandelic acid, tartaric acid, and combinations thereof.

[0031] In other aspects of the composition, the beta hydroxy acid of the booster agent can be chosen from salicylic acid, beta hydroxy-butanoic acid, and their salts.

[0032] In yet other aspects of the composition, the alpha or beta hydroxy acid comprises from about 0.0025 wt.% to about 1.25 wt.%, or 0.01 wt.% to about 0.25 wt.% , or from about 0.01 wt.% to about 0.20 wt.%, or from about 0.02 wt.% to about 0.15 wt.%, or fromabout 0.02 wt.% to about 0.1 wt.% of the booster agent. In absolute terms, from about 5% to 40% by weight, or from about 10% to about 25% by weight of the booster composition.

[0033] In some aspects of the composition, the alkyl polyglucoside of the booster agent can be chosen from Cio-16 APG, Ce-8 APG, and combinations thereof. For example, alkyl polyglucoside could be a Ce APG, Cs-io APG, coco polyglucoside (Cs-ie), lauryl polyglucoside (C12-16), and combinations thereof.

[0034] In some aspects of the composition, the alkyl polyglucoside comprises from about 0.001 wt.% to about 0.01 wt.% of the booster agent. In absolute terms, from about 0.1% to 5% by weight or from 0.5% to 2.5% by weight of the booster composition.

[0035] In some aspects of the composition, the fatty alcohol alkoxylates can be a low foaming non-ionic surfactant, for example, a C13-15 ethylene oxide (EO), butylene oxide (BO), or ethylene oxide / propylene oxide (EO / PO) surfactants; C12-14 (Ethylene Oxide) 3-5 (Propylene Oxide) 4-8 or Cs-is (Ethylene Oxide)3-5 (Propylene Oxide)3-8 (Butylene Oxide) surfactants; sorbitan surfactants such as polyoxylene sorbitan monooleates; fatty acid esters such as C5-20 alkyl fatty acid ester; and fatty alcohols such as C5-20 alkyl alcohols.

[0036] In some aspects of the composition, the fatty alcohol alkoxylates arc present in an amount of from about 0.0044 wt.% to about 2.1 wt.%, or from about 0.01 wt.% to about 0.25 wt.%, or from about 0.015 wt,% to about 0.18 wt. %, or from about 0.02 wt.% to about 0.15 wt.% of the booster agent. In absolute terms, from about 10% to 50% by weight or from 25 - 45% by weight of the booster composition.

[0037] In some aspects of the composition, the at least one acidulating agent can be chosen from sulfuric acid, phosphoric acid, methane sulfonic acids, p-toluene sulfonic acid, benzene sulfonic acid, hydrochloric acid, nitric acid and combinations thereof.

[0038] In some aspects of the composition, the one or more acidulating agents is present in an amount of from about 0.0002 wt.% to about 0.125 wt.%, or from about 0.001 wt.% to about 0.01 wt.%, or from about 0.001wt.% to about 0.008 wt.% of the booster agent. In absolute terms, from about 0.1% to about 20% by weight, or from about 1 to about 20% by weight of the booster composition.

[0039] In some aspects of the composition, the optional C1-12 carboxylic acid of the booster agent can be chosen from formic acid, acetic acid, propanoic acid, peroxy-propanoic acid, butyric acid, velaric acid, caprylic acid, caproic acid, nonanoic acid, decanoic acid,undecylenic acid, lauric acid, and combinations thereof.

[0040] In other aspects of the composition, the optional C1-12 carboxylic acid of the booster agent comprises from about 0.004 wt.% to about 2 wt.%, or from about 0.05 wt.% to about 0.20 wt.%, or from about 0.8 wt.% to about 0.15 wt.%, or from about 0.01 wt.% to about 0.1 wt.% based on a total weight of the booster agent In absolute terms this is from about 10 % to about 50% by weight, or from about 20% to about 50% by weight, or 30% to 40% by weight of the booster composition.

[0041] In yet other aspects of the composition, the composition can be used as a disinfectant.

[0042] Also provided is a method of reducing microbial contaminants of a surface of a substrate by treating the surface of the substrate with a composition that contains a peroxy acid, which is combined with a booster agent or formulation that comprises one or more alpha or beta hydroxy carboxylic acids different from the peroxy acid, one or more alkyl polyglucosides, one or more fatty alcohol alkoxylates, for example alkyl capped alcohol alkoxylates, one or more acidulating agents, and one or more C1-12 carboxylic acids different from the peroxy acid and the alpha or beta hydroxy carboxylic acids,.

[0043] In some aspects of the method, the treated substrate is used in the oil processing industries, food and beverage industries, industrial agriculture, ethanol processing, and pharmaceutical manufacturing industries.

[0044] In other aspects of the method, the substrate is used in food and beverage applications, such as in milk packaging and its derived products, dairy, cheese, juices, energy drinks, flavored water, sugar based drinks, beverages and brewery industries and derived products.

[0045] In yet other aspects of the method, the food and beverage application is a cold aseptic filling application.

[0046] In some aspects of the method, the composition is a solution that is applied to the substrate by spraying, wiping, immersion, partially dipping the substrate or submerging the substrate in a solution of the composition.

[0047] Also provided is a method of disinfecting surfaces of bottles, for example, in cold aseptic filling (CAF) applications. The method includes contacting a surface of a bottle with a composition comprising a peroxy acid that is mixed with a booster agent or formulation that contains one or more alpha or beta hydroxy carboxylic acids different from the peroxyacid, one or more alkyl polyglucoside, one or more fatty alcohol alkoxylates, for example alkyl capped alcohol alkoxylates, one or more acidulating agents, and optionally, one or more Ci-12 carboxylic acids different from the peroxy acid and the alpha or beta hydroxy carboxylic acids.

[0048] A typical CAF line processing stalls from blowing a preform into PET bottles which are then inverted and exposed to spray of the disinfectant for few seconds, followed by sterile water rinse. The bottles are continuously moving during these steps. Under a sterile environment the bottles are filled and capped with the separately disinfected caps. The caps of the bottles are separately disinfected by soaking followed by sterile water rinse. FIG. 1, depicts a typical PET Aseptic Filling Line: Process. As can be seen in Fig. 1, the PET bottles are funneled to a disinfecting staging area where disinfecting and rinsing of the bottle takes place.

[0049] In some aspects of the method, the peroxy acid and booster agent can be combined inline or separately prior to treatment of the bottle as long as the booster agent or formulation is prepared and the peroxy acid is added or combined with the booster formulation prior to applying to the surface of the bottle.

[0050] In some aspects of the method, the at least one surface of the bottle can be treated with the composition by spraying, wiping, immersion, partially dipping the substrate or submerging the substrate in a solution of composition.EXAMPLES

[0051] The following examples are intended as illustrations only. Unless otherwise noted, all parts, percentages, and ratios reported in the following examples are on a weight basis, and all reagents used in the examples were obtained, or are available, from the chemical suppliers described below, or may be synthesized by conventional techniques.

[0052] The objective of the experimental study was to arrive at the lowest possible concentration and the ratio between PAA and the new booster formulation to achieve required test results (>log 6 reduction on specified microbes).

[0053] Additional studies were done using different concentrations and ratios of PAA and Booster agent to determine that the required disinfection ( og 6 on specified microbes) can be achieved at much lower concentrations of PAA and Booster agent.

[0054] Count reduction testing was done in the laboratory using OEM standards as indicatedbelow.Preparation of spore suspension (Bacillus subtilis ATCC)

[0055] The test organisms and their stock cultures were prepared and kept in accordance with EN 12353:2013. The spore suspension was prepared in accordance with EN 13704:2002. Spore counts of stock suspension was accomplished by plating out dilutions 105, 106& 107and the initial spore count of the stock suspension was determined by a serial dilution method. The spore suspension was then stored at 5 °C for 12 months.Preparation of standard contaminated polyethylene terephthalate (PET) Bottle by point contamination method

[0056] A sealed packaged drinking water bottle was obtained. The outer surface of the bottle was sanitized with a tissue paper soaked in 70% alcohol. The bottle was aseptically opened and the water poured into a beaker. A 100 microliter (pl) aliquot of the spore suspension (spore count approximate 1 to 5 x 10A8) was added to the bottle using a pipette. The spore suspension was then agitated by shaking and tapping the bottle on a table. Additional bottles were prepared using the same technique and the bottles kept in a horizontal position with the lid of the bottle open in Bio-safety cabinet for 15 hours.Treatment to Standard contaminated PET Bottle;

[0057] A standard contaminated PET bottle was treated with an antimicrobial test solution according to the present composition for 10 seconds, and then the bottles were rinsed with water. Each test solution, was tested on at least, 3 bottles. A neutralizer, with reference to the CAF operation manual was immediately added to the bottle, the bottle shaken, and the microbiological analysis started.

[0058] A Microbiological analysis of sample was performed using a membrane filtration unit (Merck & Co., Inc, Millipore®). Place membrane filtration unit and all other requirements in the Bio-safety cabinet. Pre-solidified sterile TSA agar plates (Himedia) were labeled with sample No. and date. The membrane filtration unit was cleaned with tissue paper soaked with 70% alcohol. Sterile forceps were used to place the sterile SS frit and filter on the membrane filtration unit and a sterile funnel placed over it. The bottle with the added neutralizer was shaken, making sure the neutralizer was well mixed, and then the contents of the bottle filtered using a Millipore, EZ pack membrane filter, white gridded, 47mm, 0.45pm. After filtration a pair of sterile forceps were used to place the filter paper on the pre-solidified sterile TSA agarplate. The plate was then incubated at 30 ± 1 °C for 24 + 3 hours in an inverted position. After the incubation period, the microbial colony forming units (CFU) were counted on each filter using a colony counter (DBK-Instruments, Mumbai, Type 10DCC01).Calculation

[0059] The difference in the average initial CFU count and the average final CFU counts after disinfectant treatment was tabulated as a log reduction and reported as PASS (>10A6 cfu.ml) or FAIL

[0060] The antimicrobial efficacy was reported as an average log 10 reduction. The term “reduction” is a mathematical term used to show the relative number of live microbials being reduced from a tested area. For example, “a loglO reduction of 6” means lowering the number of the microbial count by 10A6.

[0061] Example 1 - Microbial Count Test

[0062] Following the procedure outlined above, a convention PAA formulation of PAA and Kristalle® (Solenis, LLC), a cleaner approved for use in CAF applications containing a fatty alcohol ethoxylate surfactant, was compared with a formulation containing PAA and the Booster Agent described above. The compositions were tested at different dosages and the microbial count calculated as described above. The tests measured the disinfection or reduction of B. Subtilis spores with a criteria as >log 6 reduction and contact time of 10 seconds. The results are shown in Table 1, as pass / fail at reducing the microbial counts.Table 1 - Results of Microbial Count Test, Bacillus subtilis spores

[0063] Results indicated that 1000-1500 ppm PAA in combination with 500-1500ppm of the new Booster agent provided a synergistic affect at reducing microbial activity on both tested spores (B. Subtilis and B. atropheus) . It was also observed that the current combination of2000ppm PAA + 800ppm fatty alcohol ethoxylate surfactant delivers >log6 reduction, but it fails with lower concentrations / change in ratio of PAA: surfactant.

[0064] This study also showed that the new booster agent provides a synergistic effect on reducing microbial activity when combined with PAA and can help reduce the PAA usage while delivering the desired efficacy at reducing microbial activity.Additional Embodiments

[0065] The following studies looked at the parameters for Bacillus atropheus ATCC 9372, The objective of the experimental study was to arrive at the lowest possible concentration and the ratio between PAA and the new booster formulation to achieve required test results (>log 6 reduction on specified microbes).

[0066] In addition, formulations comprising industrial benchmarks and the new composition comprising PAA and the booster agent formulation, on various non-porous surfaces including PET, glass, and metals as decsribed below.

[0067] Finally, testing on corrosion resistance and peroxide degradation rates were conducted using standard industrial compositions compared with the newly developed composition.Example 2 - Bacillus atropheus ATCC 9372Preparation of spore suspension (Bacillus atropheus ATCC 9372)

[0068] Following the process and procedures outlined above, a convention PAA formulation of PAA and Kristalle® (Solenis, EEC), a cleaner approved for use in CAF applications containing a fatty alcohol ethoxylate surfactant, was compared with a formulation containing PAA and the Booster Agent described above. The compositions were tested at different dosages and the microbial count calculated as described above. The tests measured the disinfection or reduction of Bacillus atropheus ATCC 9372 spores with a criteria as >log 6 reduction and contact time of 10 seconds. The results are shown in Table 2, as pass / fail at reducing the microbial counts.

[0069] The test organisms and their stock cultures were prepared and kept in accordance with EN 12353:2013. The spore suspension was prepared in accordance with EN 13704:2002. Spore counts of stock suspension was accomplished by plating out dilutions 106, 107 & 10S and the initial spore count of the stock suspension was determined by a serial dilution method. The spore suspension was then stored at 5 °C for 12 months.

[0070] Preparation of carriers

[0071] Sterile carriers are spot inoculated with 10 microliter (pl) aliquot of the spore suspension (spore count approximate 1 to 5 x 10A9 I mL) and let it dry for 30 min.

[0072] Treatment to carriers:

[0073] Add 100 microliter (pl) of the product on the dried carrier for required contact time and then carrier is transferred to neutralizer. Determine the microbial load in the sample by serial dilution method by incubating plates at 30 ± 1 °C for 24 + 3 hours in an inverted position. After the incubation period, the microbial colony forming units (CFU) were counted using a colony counter (DBK-Instruments, Mumbai, Type 10DCC01).

[0074] Calculation:

[0075] The difference in the CFU count of water control and the final CFU counts after disinfectant treatment was tabulated as a log reduction and reported as PASS (>10A6 cfu.ml) or FAIL as shown in Table 2 below.Table 2 - Results of Microbial Count Test, Bacillus atropheus spores

[0076] As with the B. Subtilis spores, results here indicated that 1000-2000 ppm PAA in combination with 500-800ppm of the new Booster agent provided a synergistic affect at reducing microbial activity on the B. atropheus spores.Example 3 - Corrosion Results

[0077] For the corrosion studies, benchmark formulations containing 3000ppm PAA and 2000ppm PAA in combination with 800ppm Kristallc® were compared with formulations containing lOOOppm PAA in combination with 800ppm of the booster agent composition.

[0078] The tested substrates were two different grades of stainless steel (304, 316), aluminum, EPDM rubber, and silicone. The water used for the dilution of the product was soft water having a hardness of 20ppm and TDS-36, which also serves as a reference.

[0079] Corrosion tests are carried out with metal discs (SS and Aluminium) in a Rotating Disc Corrosion Apparatus under full immersion and dynamic conditions. Standardized metal discs turn around in a product solution at a chosen temperature (60 °C) and time (28 days). The found difference in weight of the disc before and after the test is a measure of the corrosion, classified according to Corrosion Scale (mentioned on below). The discs are visually assessed for discoloration and type of corrosion (pitting, etching, etc)Testing was performed at 25 °C and 60 °C over a 4 week period.

[0080] The coiTosion rate on for all test surfaces - SS 304, 316, for the combination of adjuvant and the PAA was found similar to the benchmark of PAA + Kristalle®, not only at 25’C but also at 60’C. The change in hardness for EPDM & silicone material is similar to benchmark. Therefore, it can be concluded that the formulations containing the booster agent and PAA combination are suitable and safe for contact surfaces in food processing plants (see Table 3 below)

[0081] Results indicated at 25 ’C, the test prototypes 1500ppm PAA + 800ppm Booster agent - 32 as well as the Booster agent alone showed comparable results, with all surfaces - SS304 and 316 being suitable for use.Table 4- Temperature- 60 °C, Contact time- 1 Month, Stainless steel - 304,316

[0082] Results indicated at 60’C, the test prototypes 1500ppm PAA + 800ppm Booster agent - 32, as well as Booster agent - 32 alone showed comparable results, with all surfaces - SS304 and 316 being suitable for use.Table 5- Temperature- 60 °C, Contact time- 1 Month, Stainless steel- 304,316

[0083] Results indicated each of the formulations performed well in this study.Table 6 - Temperature- 60 °C, Contact time- 1 Month, Aluminium (soft metal)

[0084] Results indicated at 60’C, the test prototypes PAA lOOOppm + Booster agent - 32 800ppm as well as PAA lOOOppm + Booster agent - 65, and Booster agent - 32 showed comparable results, with all surfaces - Aluminum being sufficiently resistant and suitable for use.Table 7- Temperature- 60 °C, Contact time- 1 Month, EPDM Gasket

[0085] Results indicated at 60’C, the test prototypes PAA lOOOppm + Booster agent - 32 800ppm as well as PAA lOOOppm + Booster agent - 65, and Booster agent - 32, showed comparable results, with all surfaces - EPDM rubber being suitable for use. Also, the change in EPDM hardness is comparable to benchmark.Table 8 - Temperature- 60 °C, Contact time- 1 Month, Silicone Tube

[0086] Outcome - At 60’C, the test prototypes PAA 1500ppm + 800ppm Booster agent - 32 as well as PAA lOOOppm + Booster agent - 65 and Booster agent - 32 showed comparable results, with all surfaces - Silicone being suitable for use. Also, the % change in hardness is comparable to benchmarkExample 4 - PAA Degradation

[0087] PAA degradation studies were done comparing formulations using PAA alone(lOOOppm and 2000ppm), the industrial benchmark formulation (2000ppm PAA / 800ppm Kristalle®, and PAA formulated with the booster agent. The compositions were held at a temperature of 60 °C and the solutions tests over seven hours using a standard redox titration method for analyzing percent H2O2. Results can be seen in Table 9.Table 9 - PAA Degradation over Time

[0088] While at least one exemplary embodiment has been presented in the foregoing detailed description of the inventive subject matter, it should be appreciated that a vast number of variations exist. It should also be appreciated that the exemplary embodiment or exemplary embodiments are only examples, and are not intended to limit the scope, applicability, or configuration of the inventive subject matter in any way. Rather, the foregoing detailed description will provide those skilled in the art with a convenient road map for implementing an exemplary embodiment of the inventive subject matter. It being understood that various changes may be made in the function and arrangement of elements described in an exemplary embodiment without departing from the scope of the inventive subject matter as set forth in the appended claims.

Claims

We Claim:

1. A composition comprising: a peroxy acid comprising a Ci-22 carboxylic acid; and a booster agent comprising one or more alpha or beta hydroxy carboxylic acids different from the Ci-22 peroxy acid, one or more alkyl polyglucosides, one or more fatty alcohol alkoxylates, and one or more acidulating agents; and optionally, one or more C1-12 carboxylic acids different from the peroxy acid and the alpha or beta hydroxy carboxylic acids.

2. The composition according to claim 1, wherein the weight ratio of the booster agent to peroxy acid is from about 0.01:1 to about 50:1, or from about 0.01:1 to about 15:1, or from about 0.5:1 to about 2:1.

3. The composition according to claim 1 or 2, wherein the peroxy acid comprising a Ci- 22 carboxylic acid is chosen from peracetic acid, performic acid, peroxypentanoic acid, peroxyhexanoic acid, peroxyheptanoic acid, peroxyoctanoic acid, peroxynonanoic acid, peroxy decanoic acid, peroxyundecanoic acid, peroxydodecanoic acid, perglycolic acid, peroxy ascorbic acid, peroxy lactic acid, peroxyadipic acid, peroxycitric acid, peroxypimelic acid, peroxysuberic acid, peroxy-propanoic acid, and combinations thereof. Add4. The composition according to any one of claims 1-3, wherein the peroxy acid comprising a C1-22 carboxylic acid is present in an amount of from about 0.05 wt.% to about 0.20 wt.%, or from about 0.1 wt.% to about 0.15 wt.% based on a total weight of the composition.

5. The composition according to any one of claims 1-4, wherein the optional C1-12 carboxylic acid is chosen from formic acid, acetic acid, propanoic acid, butyric acid, velaricacid, caprylic acid, caproic acid, nonanoic acid, decanoic acid, undecylenic acid, lauric acid, and combinations thereof.

6. The composition according to any one of claims 1-5, wherein the optional Ci-12 carboxylic acid is present in an amount of from about 0.004 wt.% to about 2 wt.%, or from about 0.05 wt.% to about 0.20 wt.%, or from about 0.8 wt.% to about 0.15 wt.%, or from about 0.01 wt.% to about 0.1 wt.% based on a total weight of the booster agent.

7. The composition according to any one of claims 1-6, wherein the alpha and beta hydroxy acids are chosen from lactic acid, glycolic acid, citric acid, malic acid, mandelic acid, tartaric acid, salicylic acid, beta hydroxybutanoic acid, and salts thereof.

8. The composition according to any one of claims 1-8, wherein the alpha and / or beta hydroxy acid is present and one or both is present in a total amount of from about 0.0025 wt.% to about 1.25 wt.%, or 0.01 wt.% to about 0.25 wt.% , or from about 0.01 wt.% to about 0.20 wt.%, or from about 0.02 wt.% to about 0.15 wt.%, or from about 0.02 wt.% to about 0.1 wt.% based on a total weight of the booster agent.

9. The composition according to any one of claims 1-8, wherein the alkyl polyglucosides is chosen from Cio-16 APG, Ce-s APG, and combinations thereof.

10. The composition according to any one of claims 1-9, wherein the alkyl polyglucoside of the booster agent can be chosen from Cio-16 APG, Ce-s APG, and combinations thereof. For example, alkyl polyglucoside could be a Ce APG, Cs-io APG, coco polyglucoside (Cs-ie), lauryl polyglucoside (C12-16), and combinations thereof.

11. The composition according to any one of claims 1-10, wherein the one or more fatty alcohol alkoxylates is a non-ionic surfactant chosen from C13-15 ethylene oxide, butylene oxide, or ethylene oxide / propylene oxide surfactants; C12-14 (EO)3-5 (PO)4-6 or similar Cs-is (EO)3-5 (PO)3-8 (BO) surfactants; sorbitan surfactants; fatty acid esters; fatty alcohols and combinations thereof.

12. The composition according to any one of claims 1-11, wherein the one or more fatty alcohol alkoxylates is present in an amount of from about 0.0044 wt.% to about 2.1 wt.%, or from about 0.01 wt.% to about 0.25 wt.%, or from about 0.015 wt,% to about 0.18 wt. %, or from about 0.02 wt.% to about 0.15 wt.% based on a total weight of the booster agent.

13. The composition according to any one of claims 1-12, wherein the acidulating agent is chosen from sulfuric acid, phosphoric acid, methane sulfonic acids, p-toluene sulfonic acid, benzene sulfonic acid, hydrochloric acid and nitric acid, and combinations thereof.

14. The composition according to any one of claims 1-13, wherein the acidulating agent is present in an amount of from about 0.0002 wt.% to about 0.125 wt.%, or from about 0.001 wt.% to about 0.01 wt.%, or from about 0.001wt.% to about 0.008 wt.% based on a total weight of the booster agent.

15. A method of reducing microbial contaminants of a surface of a substrate comprising: treating the surface of the substrate with a composition according to claim 1.

16. The method according to claim 15, wherein the substrate is used in the oil processing industries, food and beverage industries, industrial agriculture, ethanol processing, and pharmaceutical manufacturing industries.

17. The method according to claim 15 or 16, wherein the food and beverage industry is a cold aseptic filling application.

18. A method of disinfecting surfaces of bottles in cold aseptic filling machines comprising: contacting a surface of a bottle with the composition according to claim 1.

19. The method according to claim 18, wherein the step of contacting is further defined as spraying, wiping, immersing, partially dipping the substrate or submerging the substrate with the composition.

20. The method according to claim 18 or 19, wherein the surface of the bottle is rinsed after being contacted with the composition according to claim 1.

Images