Intelligent pollen monitoring and analysis system and control method therefor

Abstract

An intelligent pollen monitoring and analysis system and a control method therefor. The intelligent pollen monitoring and analysis system comprises a monitoring cabinet (1), wherein a control module (7) and a supporting platform (13) are disposed in the monitoring cabinet (1), and a slide storage rack (2), a pollen sampling assembly (4), an image collection apparatus (5) and a microscope slide transfer mechanism (9) are mounted on the supporting platform (13); the slide storage rack (2) is disposed on a rotating mechanism (3), and a fine-tuning mechanism (6) is disposed on the image collection apparatus (5); and the rotating mechanism (3), the pollen sampling assembly (4), the image collection apparatus (5), the microscope slide transfer mechanism (9) and the fine-tuning mechanism (6) are all connected to the control module (7), and the control module (7) is used for controlling the starting and stopping of components connected thereto, identifying an image uploaded by the image collection apparatus (5) to obtain the concentration of pollen and / or the pollen count, and sending the image, the concentration and / or the pollen count to an external management terminal. Therefore, the present application solves the problem of inaccurate monitoring of the pollen concentration due to the shaking of an existing pollen monitoring apparatus which uses a sampling tape for pollen transportation.

Description

Pollen intelligent monitoring and analysis system and control method thereof Technical Field

[0001] The present invention relates to the field of pollen monitoring, and in particular to an intelligent pollen monitoring and analysis system and a control method thereof. Background Art

[0002] With the rise in urban greening rates, airborne pollen pollution is becoming increasingly serious, and the incidence of hay fever is on the rise, affecting 10% to 30% of the global population. In my country, pollen allergies affect 1% to 6% of the population. Plants in bloom produce pollen. Pollen grains are large, lightweight, and 15 to 30 μm in diameter, easily transported by airborne aerosols. This form of pollen is commonly referred to as airborne pollen. When inhaled, the allergenic proteins in airborne pollen can cause symptoms of pollen allergy, such as runny nose, tearing, itchy skin, and asthma.

[0003] The existing technology uses a pollen online monitoring system (CN201510731452.1) to monitor pollen, based on the type and quantity of pollen, to remind relevant meteorological departments to issue relevant warning reminders and take measures to reduce pollen floating, so as to guide people with pollen allergies to reduce activities in areas with high pollen index and alleviate allergic symptoms.

[0004] When collecting pollen, the existing online pollen monitoring system uses a sampling membrane tape to sample pollen, and then uses a conveyor belt-like method to transport the sampling membrane tape to a microscope for photography. The left shaft then rolls up the membrane tape that has collected and observed the pollen sample. Although this method can achieve online monitoring of pollen, there will be jitter when the sampling membrane tape is transported using a conveyor belt. If the pollen collected on the sampling membrane tape is relatively dense, the pollen will be shaken to two adjacent sampling areas on the sampling membrane tape, thereby affecting the refined collection of pollen concentration. The sampling membrane tape that has been photographed is rolled up, and the pollen on the membrane tape will interfere with each other, making the sampling membrane tape unable to be recycled. When there is doubt about the collected data, it cannot be traced. Summary of the Invention

[0005] In view of the above-mentioned deficiencies in the prior art, the intelligent pollen monitoring and analysis system and control method thereof provided by the present invention solve the problem that the existing pollen monitoring device uses a sampling membrane belt for pollen transmission, which causes jitter and inaccurate pollen concentration monitoring.

[0006] In order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention is:

[0007] In a first aspect, a pollen intelligent monitoring and analysis system is provided, comprising a monitoring box, wherein a control module and a support platform are provided within the monitoring box, wherein the support platform is mounted with a slide storage rack for storing a plurality of non-overlapping slides, a pollen sampling assembly for drawing in ambient air and collecting pollen in the air onto the slides therein by a volumetric method, an image acquisition device for amplifying the pollen image on the slides and then acquiring the image, and a slide transport mechanism for grabbing the slides on the slide storage rack and moving them to the pollen sampling assembly and the image acquisition device;

[0008] The slide storage rack is set on the rotating mechanism, and the image acquisition device is provided with a fine-tuning mechanism for adjusting the slide in the XYZ three directions; the rotating mechanism, pollen sampling assembly, image acquisition device, slide transfer mechanism and fine-tuning mechanism are all connected to the control module, and the control module is used to control the start and stop of the components connected to it and to identify the image uploaded by the image acquisition device to obtain the pollen concentration and / or pollen number, and send the image and concentration and / or pollen number to the external management end.

[0009] Furthermore, the pollen sampling assembly includes a base plate and a pollen sampling chamber formed by the engagement of a glass cover and a buffer plate. A vertically extending vertical plate is mounted on the base plate. The glass cover is fixed to the vertical plate. A linear guide rail that slides with the buffer plate is provided on the vertical plate below the glass cover. A through-type stepper motor that engages and disengages the buffer plate from the glass cover is mounted on the base plate.

[0010] A glass slide table is installed on the inner surface of the buffer plate and slides relative to the buffer plate along the X-axis direction; a driving mechanism is fixed on the glass slide cover to slide the glass slide table; the top of the glass slide cover is connected to an air inlet pipe extending from the monitoring box to introduce external air and impact the glass slide, and an exhaust hole connected to the exhaust fan is installed on the side wall of the glass slide cover; the through-type stepper motor and the driving mechanism are both electrically connected to the control module.

[0011] Furthermore, the lower surface of the buffer plate is connected to a pressure plate through a plurality of optical axis screws, and the diameter of the hole on the pressure plate for the optical axis screw to pass through is larger than the diameter of the optical axis screw; the pressure plate is fixedly connected to the screw rod of the through-type stepper motor, and a spring is mounted on the optical axis screw between the pressure plate and the buffer plate.

[0012] Furthermore, the driving mechanism includes a driving motor fixed to the outer surface of the side wall of the glass slide cover, the screw shaft of the driving motor passes through the sealing plate and penetrates into the glass slide cover, the screw shaft is fitted with a screw nut I, and the screw nut I has a limit plate extending vertically toward the buffer plate; a limit groove is provided on the side of the glass slide placement table, and when the buffer plate is buckled with the glass slide cover, the limit plate extends into the limit groove; the driving motor is electrically connected to the control module.

[0013] Furthermore, the slide transfer mechanism includes a gripper and a rotating table that drives the gripper to rotate 360°. A support block is fixed on the rotating table, and a first lifting mechanism that drives the gripper to rise and fall in the vertical direction is installed on the support block. A lifting platform is provided on the first lifting mechanism, and a first moving mechanism that drives the gripper to move linearly on the horizontal plane is installed on the lifting platform. The rotating table, the first lifting mechanism and the first moving mechanism are all connected to the control module.

[0014] Furthermore, the gripper includes a plate-like body fixed on the first moving mechanism, and the non-mounting end of the plate-like body is forked and U-shaped; the two arms of the U-shape are forked into at least two extension strips for supporting the glass slide, and the outermost extension strip is provided with an L-shaped groove that contacts the edge of the glass slide; the non-outermost extension strip is provided with a receiving groove with a length equal to the maximum width of the glass slide for supporting the glass slide.

[0015] Furthermore, the image acquisition device includes a microscope without an eyepiece, a camera electrically connected to the control module is installed at the installation position of the eyepiece of the microscope, and the fine-tuning mechanism is installed on the mirror arm of the microscope;

[0016] The fine-tuning mechanism includes a slide carrier, an L-shaped slide, and a second lifting mechanism fixed to the microscope arm for raising and lowering the microscope stage along the Z axis. The short side of the slide is provided with a boss parallel to its long side. The boss is located in a Y-axis groove provided on the lower surface of the stage. The mounting plate on the long side of the slide extends upward from the side of the stage, with its top surface located above the stage.

[0017] The slide is fixed on the second moving mechanism on the mirror arm and slides in the slide groove through the second moving mechanism; a third moving mechanism is fixed on the side of the mounting plate facing the stage, which carries the glass slide carrier and slides along the X-axis direction.

[0018] Furthermore, the glass slide carrier includes an L-shaped slide, the short side of the slide is fixed on the screw nut II of the third moving mechanism, the long side of the slide extends along the length direction of the screw of the third moving mechanism, and a support plate with two cantilevers is fixed on it, and a card slot for loading glass slides is opened on the cantilever.

[0019] Furthermore, the control module includes a micro host and an electrical box that are interconnected, and the rotating mechanism, pollen sampling component, image acquisition device, glass slide transport mechanism and fine-tuning mechanism are all connected to the electrical box and the backup power supply; the micro host is embedded with an execution program for controlling the rotating mechanism, pollen sampling component, image acquisition device, glass slide transport mechanism and fine-tuning mechanism; the micro host is connected to an input module placed in the monitoring box, and the input module is used to adjust the embedded execution program; a display electrically connected to the micro host is fixed on the inner side of the door panel of the monitoring box.

[0020] Furthermore, the monitoring box is also equipped with a temperature sensor, a humidity sensor, an air conditioner that keeps the internal temperature of the monitoring box constant at a preset temperature, and a dehumidifier that dehumidifies when the internal humidity of the monitoring box is greater than the preset humidity. The temperature sensor, humidity sensor, air conditioner and dehumidifier are all electrically connected to the control module.

[0021] In a second aspect, a control method for a pollen intelligent monitoring and analysis system is provided, comprising the steps of:

[0022] C1. Determine whether the pollen sampling assembly has completed pollen collection on at least one glass slide. If so, proceed to step C2; otherwise, proceed to step C4.

[0023] C2. Read the number of sampling areas on the glass slide in the pollen sampling assembly and the sampling time of the last sampling area, and calculate the difference between the cut-off sampling time of the last sampling area and the cut-off time of the total sampling time;

[0024] C3. When the difference is equal to zero, open the pollen sampling assembly, move the glass slide with pollen collected to the position where it was initially stored on the glass slide storage rack, and then proceed to step C4. When the difference is greater than zero, proceed to step C4;

[0025] C4. At the sampling start time of the slide to be sampled, the serial number of the slide to be sampled is read, and the rotating mechanism is started to rotate the slide with the corresponding serial number on the slide storage rack to the grabbing side of the slide transfer mechanism;

[0026] C5. Start the slide transport mechanism to grab the slide with the corresponding serial number and move it into the open pollen sampling assembly, and then close the pollen sampling assembly;

[0027] C6. The pollen sampling component moves the sampling area on the glass slide to align with the air inlet according to the number of sampling areas on the glass slide and the sampling time of each sampling area, and draws air to hit the corresponding area on the glass slide for pollen sampling.

[0028] The beneficial effects of the present invention are as follows: this solution uses a glass slide to collect pollen, and then cooperates with a glass slide transfer mechanism to grab the glass slide to transfer the glass slide. During the transfer process, a clamping method is used to transfer a single glass slide. There is no jitter during the transfer process, which can prevent pollen from entering two adjacent sampling areas, thereby ensuring the accuracy of pollen concentration and quantity detection.

[0029] This solution places the slides that have completed image acquisition on a slide storage rack. There is no overlap between the slides, and the pollen on the slides will not affect each other. The slides can be recycled and stored. If data anomalies are found in subsequent scientific research, the slides used for acquisition can be found to facilitate traceability.

[0030] This solution uses a glass slide cover and a buffer plate to form a pollen sampling chamber. Combined with a through-type stepper motor, the buffer plate can be quickly raised and lowered to achieve rapid placement and removal of the glass slide. When the buffer plate is fully in contact with the glass slide cover, the continued movement of the through-type stepper motor will continue to move the pressure plate upward. The elastic force generated by the compression spring can apply a certain amount of pressure to the buffer plate to improve the sealing of the pollen sampling chamber, thereby preventing external air leakage from contaminating the sampled material and causing the actual flow rate at the nozzle to be less than the set value. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 is a three-dimensional view of the pollen intelligent monitoring and analysis system with the cabinet door opened.

[0032] Figure 2 is a three-dimensional diagram of the slide storage rack, pollen sampling assembly, image acquisition device and slide transport mechanism installed on the support platform.

[0033] FIG3 is a perspective view of the pollen sampling assembly with the buffer plate in an open state.

[0034] FIG4 is a cross-sectional view of the pollen sampling assembly when the buffer plate is in an open state.

[0035] FIG5 is an enlarged view of portion A in FIG4 .

[0036] FIG6 is a perspective view of a slide storage rack.

[0037] FIG7 is a perspective view of the slide transport mechanism.

[0038] FIG8 is an enlarged view of portion B in FIG7 .

[0039] FIG9 is a perspective view of an image acquisition device.

[0040] FIG10 is an enlarged view of portion C in FIG9 .

[0041] FIG11 is a three-dimensional diagram of the pollen intelligent monitoring and analysis system in FIG1 with the upper plate inside the box removed.

[0042] FIG12 is a flow chart of a control method of the pollen intelligent monitoring and analysis system.

[0043] Among them, 1. Monitoring box; 11. Door panel; 12. Support legs; 13. Support platform; 2. Slide storage rack; 21. Connecting shaft; 22. Platform plate; 221. Support arm; 2211. Protrusion; 3. Rotating mechanism; 4. Pollen sampling assembly; 41. Pollen sampling chamber; 411. Slide cover; 4111. Exhaust hole; 412. Buffer plate; 4121. Optical axis screw; 4122. Pressure plate; 413. Slide placement table; 4131. Limiting groove; 4132. Sliding block; 4133. Slide limiting plate; 414. Inlet pipe; 415. Hall sensor; 42. Bottom plate; 421. Vertical plate; 422. Through-type stepping motor; 43. Driving mechanism; 431. Driving motor; 432. Screw shaft; 433. Screw nut I; 4331. Limiting plate; 5. Image acquisition device; 51. Microscope; 511. Mirror arm; 52. Camera; 53. Stage; 6. Fine-tuning mechanism; 61. Slide carrier; 611. Slide plate; 612. Support plate; 6121. Cantilever; 62. Slide; 621. Mounting plate; 63. Second lifting mechanism; 64. Second moving mechanism; 65. Third moving mechanism; 651. Screw nut II; 7. Control module; 71. Display; 72. Electrical box; 73. Backup power supply; 74. Input module; 75. Dehumidifier; 8. Glass slide; 9. Glass slide transfer mechanism; 91. Gripper; 911. Plate-shaped body; 912. Extension bar; 92. Rotating table; 93. Support block; 94. First lifting mechanism; 95. Lifting platform; 96. First moving mechanism. DETAILED DESCRIPTION

[0044] The specific embodiments of the present invention are described below to facilitate understanding of the present invention by those skilled in the art. However, it should be clear that the present invention is not limited to the scope of the specific embodiments. For those skilled in the art, as long as various changes are within the spirit and scope of the present invention as defined and determined by the appended claims, these changes are obvious, and all inventions and creations utilizing the concepts of the present invention are protected.

[0045] As shown in Figures 1 and 11, the pollen intelligent monitoring and analysis system provided by this solution includes a monitoring box 1. Preferably, the monitoring box 1 is a box body with an openable and closable door panel 11, and a plurality of supporting legs 12 are provided at the bottom thereof so that the monitoring box 1 is at a certain distance from the ground to reduce the interference of the ground environment on the monitoring box 1.

[0046] As shown in Figure 2, a control module 7 and a support platform 13 are provided in the monitoring box 1. The support platform 13 is equipped with a slide storage rack 2 for storing multiple non-overlapping slides 8, a pollen sampling component 4 for inhaling external ambient air and collecting pollen in the air onto the slide 8 inside it by a volumetric method, an image acquisition device 5 that amplifies the pollen image on the slide 8 and performs image acquisition, and a slide transfer mechanism 9 for grabbing the slide 8 on the slide storage rack 2 and moving it to the pollen sampling component 4 and the image acquisition device 5.

[0047] As shown in Figure 6, the slide storage rack 2 is set on the rotating mechanism 3. The rotating mechanism 3 can adopt a relatively mature high-precision electric turntable in the existing technology, or it can adopt a servo electric drive worm gear, and then transmit power to a platform fixed on the worm gear, and the slide storage rack 2 is placed on the platform.

[0048] The slide rack 2 is a multi-layer structure formed by connecting multiple platforms 22 via connecting shafts 21. In this embodiment, each platform 22 preferably has four supporting arms 221 on each side for placing slides 8. These supporting arms 221 have protrusions 2211 to prevent the slides 8 from slipping. In this embodiment, the structure for placing slides 8 on the platform 22 is configured as supporting arms 221, which facilitates the slide transport mechanism 9 to lift the slides 8 from below, allowing for easy placement and removal of the slides 8.

[0049] As shown in FIG9 , the image acquisition device 5 is provided with a fine-tuning mechanism 6 for adjusting the slide 8 in three directions, X, Y, and Z. The X-axis and Y-axis mentioned in this solution are located on a horizontal plane, and the Z-axis is located on a vertical plane. For details, please refer to the coordinate directions in FIG4 . The image acquisition device 5 includes a microscope 51 without an eyepiece. A camera 52 electrically connected to the control module 7 is installed at the installation position of the eyepiece of the microscope 51. The fine-tuning mechanism 6 is installed on the mirror arm 511 of the microscope 51.

[0050] The fine-tuning mechanism 6 includes a glass slide carrier 61, an L-shaped slide 62, and a second lifting mechanism 63 fixed to the mirror arm 511, which is used to lift and lower the stage 53 of the microscope 51 along the Z axis; the short side of the slide 62 is provided with a boss parallel to its long side, and the boss is located in a slide groove along the Y axis direction opened on the lower surface of the stage 53; the mounting plate 621 of the long side of the slide 62 extends upward from the side of the stage 53, and its top surface is located above the stage 53.

[0051] The slide 62 is fixed to the second moving mechanism 64 on the mirror arm 511 and slides in the slide groove through the second moving mechanism 64; a third moving mechanism 65 is fixed on the side of the mounting plate 621 facing the stage 53, which carries the glass slide carrier 61 and slides along the X-axis direction.

[0052] As shown in Figure 10, the glass slide carrier 61 includes an L-shaped slide 611, the short side of the slide 611 is fixed on the screw nut II 651 of the third moving mechanism 65, and the long side of the slide extends along the length direction of the screw of the third moving mechanism 65, and a support plate 612 with two cantilevers 6121 is fixedly installed thereon, and a card slot for loading the glass slide 8 is opened on the cantilever 6121.

[0053] The second lifting mechanism 63 of the image acquisition device 5 can be an electric push rod or a servo motor that drives a worm gear. This power is transmitted to a worm with threads in the middle and upper sections. As the worm rotates, it causes the nut attached to its threaded section to slide up and down, driving the stage 53 connected to the nut up and down. The second lifting mechanism 63 can also be combined with the microscope's existing rack and pinion lifting mechanism, replacing the existing right-side coarse and fine focus knob with a stepper motor to perform the stage's up and down automatic focusing function. Both the second moving mechanism 64 and the third moving mechanism 65 utilize a motor-screw-nut structure, a relatively mature structure in the prior art and will not be further described here.

[0054] The rotating mechanism 3, pollen sampling assembly 4, image acquisition device 5, slide transport mechanism 9 and fine-tuning mechanism 6 are all connected to the control module 7. The control module 7 is used to control the start and stop of the components connected thereto and to identify the image uploaded by the image acquisition device 5 to obtain the concentration and / or pollen number of the pollen, and to send the image and the concentration and / or pollen number to the external management end.

[0055] This solution obtains the concentration and / or number of pollen through image recognition, which is achieved by using relatively mature image recognition methods in the existing technology, and can also be achieved by using a trained neural network model; these are relatively mature technologies in the existing technology and will not be repeated here.

[0056] Referring to Figure 1 again, the control module 7 includes a micro host and an electrical box 72 that are interconnected. The rotating mechanism 3, the pollen sampling component 4, the image acquisition device 5, the slide transport mechanism 9 and the fine-tuning mechanism 6 are all connected to the electrical box 72 and the backup power supply 73; the backup power supply 73 can provide a short period of power when the pollen intelligent monitoring and analysis system suddenly loses power, so that it can save the data that is being run or processed, and avoid the loss of part of the data being identified due to sudden power outage.

[0057] The micro host is embedded with an execution program for controlling the rotating mechanism 3, the pollen sampling component 4, the image acquisition device 5, the slide transport mechanism 9 and the fine-tuning mechanism 6; the micro host is connected to the input module 74 placed in the monitoring box 1, and the input module 74 is used to adjust the embedded execution program; the inner side of the door panel 11 of the monitoring box 1 is fixed with a display 71 electrically connected to the micro host.

[0058] The setting of the input module 74 and the display 71 can facilitate the management personnel to update the execution program inside the micro host to ensure that the rotating mechanism 3, pollen sampling component 4, image acquisition device 5, slide transport mechanism 9 and fine-tuning mechanism 6 use the best method to perform pollen intelligent monitoring.

[0059] The monitoring box 1 is also equipped with a temperature sensor, a humidity sensor, an air conditioner that keeps the internal temperature of the monitoring box 1 constant at a preset temperature, and a dehumidifier 75 that dehumidifies when the internal humidity of the monitoring box 1 is greater than the preset humidity. The temperature sensor, humidity sensor, air conditioner and dehumidifier 75 are all electrically connected to the control module 7.

[0060] The air conditioner can make the various components in the monitoring box 1 operate at a constant temperature, ensuring the normal operation of each component in a sealed environment. The dehumidifier 75 can remove moisture in the monitoring box 1 to avoid affecting the operation of various internal electrical components in relatively humid weather.

[0061] As shown in Figure 3, the pollen sampling assembly 4 provided in this solution includes a base plate 42 and a pollen sampling chamber 41 formed by snapping together a glass cover 411 and a buffer plate 412. A vertical plate 421 extending in the vertical direction is installed on the base plate 42. The glass cover 411 is fixed on the vertical plate 421, and a linear guide rail slidingly engaged with the buffer plate 412 is provided on the vertical plate 421 below the glass cover 411. The linear guide rail here is arranged in the vertical direction, and the buffer plate 412 is fixed on the slider of the linear guide rail.

[0062] A through-type stepping motor 422 is mounted on the bottom plate 42 , and is coupled with / separated from the buffer plate 412 and the glass cover 411 .

[0063] A glass slide placement table 413 is installed on the inner surface of the buffer plate 412 and slides relative to the buffer plate 412 along the X-axis direction; a driving mechanism 43 is fixed on the glass slide cover 411, which slides with the glass slide placement table 413; the top of the glass slide cover 411 is connected to an air intake pipe 414 that introduces external air and impacts the glass slide 8, and the end of the air intake pipe 414 located in the pollen sampling chamber 41 is connected to a rectangular sampling nozzle; an exhaust hole 4111 connected to an exhaust fan is installed on the side wall of the glass slide cover 411.

[0064] As shown in Figure 5, the lower surface of the buffer plate 412 is connected to a pressure plate 4122 through a plurality of optical axis screws 4121. The diameter of the hole on the pressure plate 4122 for the optical axis screw 4121 to pass through is larger than the diameter of the optical axis screw 4121; the pressure plate 4122 is fixedly connected to the screw rod of the through-type stepping motor 422, and a spring is installed on the optical axis screw 4121 between the pressure plate 4122 and the buffer plate 412.

[0065] After adopting the above structure, this solution can drive the step-by-step movement of the two components through a power component (through-type stepping motor 422), which can not only ensure the accurate alignment and fastening of the glass cover 411 and the buffer plate 412, but also apply pressure to the fastened buffer plate 412 through the spring, thereby improving the sealing of the pollen sampling chamber 41.

[0066] As shown in Figure 4, the driving mechanism 43 includes a driving motor 431 fixed to the outer surface of the side wall of the glass cover 411. The screw shaft of the driving motor 431 passes through the sealing plate and penetrates into the glass cover 411. The screw shaft 432 is fitted with a screw nut I 433, and the screw nut I 433 has a limit plate 4331 extending vertically toward the buffer plate 412; a limit groove 4131 is provided on the side of the glass slide placement table 413. When the buffer plate 412 is engaged with the glass slide cover 411, the limit plate 4331 extends into the limit groove 4131.

[0067] In this solution, the driving mechanism 43 and the components that need to be moved by it are arranged on two separate structures. In this way, when the through-type stepping motor 422 is working, it only needs to move the relatively light components, and the through-type stepping motor 422 with relatively large power is not required, which reduces the equipment cost investment to a certain extent; furthermore, this solution arranges the driving motor 431 on the outside of the glass cover 411, which can prevent pollen from adhering to the driving motor 431 and affecting the driving motor 431, and can also greatly reduce the internal volume of the pollen sampling chamber 41.

[0068] When the glass slide placement table 413 is moved, this solution cleverly cooperates with the limiting groove 4131 and the limiting plate 4331, which are in contact but not fixed. This not only enables the glass slide placement table 413 located on the buffer plate 412 to quickly move in coordination with the screw shaft 432, but also when the buffer plate 412 descends, the limiting plate 4331 can be quickly separated from the limiting groove 4131 without damaging the structure of the screw shaft 432.

[0069] During implementation, this solution preferably adopts a glass slide placement table 413 including a sliding block 4132 and a glass slide limiting plate 4133 fixed on the sliding block 4132. The sliding block 4132 is adjacent to the lower surface of the vertical plate 421 and is provided with a strip groove along the X direction that cooperates with the linear guide rail on the bottom plate 42. The glass slide limiting plate 4133 is provided with a groove body for placing the glass slide 8, and the bottom of the groove body has a hollow structure for avoiding the hand claw 91 for transporting the glass slide 8; the limiting groove 4131 is provided on the extension plate of the glass slide limiting plate 4133 toward the driving motor 431; wherein the height of the sliding block 4132 is greater than the thickness of the hand claw 91 for transporting the glass slide 8.

[0070] When the linear guide rail is installed, it is close to the wall of the strip groove. After installation, a certain friction resistance will be generated to prevent the sliding block 132 from moving due to slight shaking and affecting the positioning of the limit groove. Limits are set at both ends of the linear guide rail on the base plate 42 to prevent the sliding block 4132 from falling off.

[0071] The sliding block 4132 can create a certain distance between the glass slide limiting plate 4133 and the buffer plate 412, so that the gripper 91 for transporting the glass slide 8 can smoothly enter under the glass slide limiting plate 4133 to place the glass slide 8 in the open trough.

[0072] During implementation, this scheme preferably sets the upper part of the limit groove 4131 as a trumpet mouth that widens outward, the wall surface of the lower end is perpendicular to the glass slide placement table 413, and the width of the lower end is equal to the thickness of the limit plate 4331; the upper end structure can ensure that the limit plate 4331 accurately enters the limit groove 4131, and the lower structure can prevent the glass slide placement table 413 and the screw nut I 433 from loosening during the movement, affecting the precise control of the displacement of the slide 8.

[0073] During implementation, this solution preferably has a magnet embedded on the side of the screw nut I 433 opposite to the limit plate 4331, and a Hall sensor 415 electrically connected to the control module 7 is installed on the side wall of the glass cover 411. The magnet and the Hall sensor 415 cooperate to limit the movement of the screw nut I 433.

[0074] As shown in Figure 7, the slide transfer mechanism 9 includes a gripper 91 and a rotating table 92 that drives the gripper 91 to rotate 360°. A support block 93 is fixed on the rotating table 92. A first lifting mechanism 94 that drives the gripper 91 to rise and fall in the vertical direction is installed on the support block 93. A lifting platform 95 is provided on the first lifting mechanism 94. A first moving mechanism 96 that drives the gripper 91 to move linearly on a horizontal plane is installed on the lifting platform 95. The rotating table 92, the first lifting mechanism 94 and the first moving mechanism 96 are all connected to the control module 7.

[0075] The structure of the rotating table 92 can be exactly the same as that of the rotating mechanism 3, or it can be slightly simplified, and a servo motor can be directly used to rotate the rotating table; the first lifting mechanism 94 and the first moving mechanism 96 both use a motor screw nut structure.

[0076] As shown in FIG8 , gripper 91 includes a plate-like body 911 fixed to first moving mechanism 96 . The non-mounting end of plate-like body 911 is forked and U-shaped. The two arms of the U-shape branch into at least two extension bars 912 for supporting glass slide 8 . The outermost extension bar 912 has an L-shaped groove that contacts the edge of glass slide 8 . The non-outermost extension bar 912 has a receiving groove with a length equal to the maximum width of glass slide 8 for supporting glass slide 8 . In this embodiment, the outermost extension bar 912 is preferably shorter than the remaining extension bars 912 .

[0077] After the gripper 91 is set to the above structure, the L-shaped groove and the receiving groove can stably limit the slide 8, so that when the slide 8 is transported, the slide 8 can be prevented from sliding or shaking, so as to avoid the pollen adhered to the slide 8 from running away.

[0078] Referring to FIG. 12 , it shows a flow chart of a control method of the pollen intelligent monitoring and analysis system. As shown in FIG. 1 , the method C includes steps C1 to C6 .

[0079] In step C1, it is determined whether the pollen sampling assembly 4 has completed pollen collection on at least one glass slide 8. If so, the process proceeds to step C2; otherwise, the process proceeds to step C4.

[0080] In step C2, the number of sampling areas of the glass slide 8 in the pollen sampling assembly 4 and the sampling time of the last sampling area are read, and the difference between the cut-off sampling time of the last sampling area and the cut-off time of the total sampling time is calculated;

[0081] In step C3, when the difference is equal to zero, the pollen sampling assembly 4 is opened, and the glass slide 8 with pollen collected is moved to the position where it is initially stored on the glass slide storage rack 2, and then step C4 is entered. When the difference is greater than zero, step C4 is entered;

[0082] In step C4, at the sampling start time of the glass slide 8 to be sampled, the serial number of the glass slide 8 to be sampled is read, and the rotating mechanism 3 is started to rotate the glass slide 8 with the corresponding serial number on the glass slide storage rack 2 to the grabbing side of the glass slide transfer mechanism 9;

[0083] In step C5, the slide transport mechanism 9 is activated to grab the slide 8 with the corresponding serial number and move it into the opened pollen sampling assembly 4, and the pollen sampling assembly 4 is closed;

[0084] In step C6, the pollen sampling component 4 moves the sampling area on the glass slide 8 to align with the air inlet according to the number of sampling areas on the glass slide 8 and the sampling time of each sampling area, and draws air to hit the corresponding area of ​​the glass slide 8 for pollen sampling.

[0085] In one embodiment of the present invention, the control method of the pollen intelligent monitoring and analysis system further includes:

[0086] D1, determine whether the last glass slide 8 that has completed pollen collection is located on the grabbing side of the glass slide transfer mechanism 9. If so, proceed to step D3; otherwise, proceed to step D2;

[0087] D2, start the rotating mechanism 3 to rotate the slide storage rack 2, so that the previous slide 8 with pollen collection completed is rotated to the grabbing side of the slide transfer mechanism 9;

[0088] D3, grab the glass slide 8 on which pollen has been collected, and transport it to the image acquisition device 5, and collect multiple images of each sampling area of ​​the glass slide 8 in turn;

[0089] D4. When multiple pieces of image information have been collected for each sampling area of ​​the glass slide 8, the glass slide 8 is moved to the position where it is initially stored on the glass slide storage rack 2.

[0090] This solution uses a glass slide 8 to collect pollen, and then cooperates with the glass slide transfer mechanism 9 to grab the glass slide 8 to transfer the glass slide 8. During the transfer process, a clamping method is used to transfer a single glass slide 8. There is no jitter during the transfer process, which can prevent pollen from entering two adjacent sampling areas, thereby ensuring the accuracy of pollen concentration and type detection.

[0091] During implementation, the preferred control method of the pollen intelligent monitoring and analysis system of this solution further includes adaptively adjusting the air flow entering the pollen sampling assembly 4:

[0092] E1. When the exhaust fan is delivering air to the pollen sampling assembly 4, the flow rate value at the air inlet of the pollen sampling assembly 4 is collected;

[0093] E2. Determine whether the flow rate value is within the preset flow rate range. If so, proceed to step E7; otherwise, proceed to step E3.

[0094] E3. Calculate the flow error value e, the flow error cumulative value i, and the flow error change rate d in the PID controller based on the flow value: e = sf, i = i0 + e, d = ep

[0095] Where s is the preset flow threshold; f is the flow value; p is the last flow error value; i0 is the accumulated flow error value corresponding to the last flow judgment;

[0096] E4. According to the flow error value, adjust the proportional coefficient q corresponding to the flow in the PID controller l , integral coefficient u l and the differential coefficient w l :

[0097] When e<1, q l =q l0 ,u l =u l0 , w l =w l0 *0.5; when e≥1, q l =q l0 *2,u l =u l0 , w l =wl0 ;

[0098] Among them, q l0 is the proportional coefficient threshold; u l0 is the integral coefficient threshold; w l0 is the differential coefficient threshold;

[0099] E5. According to e, i, d and q l 、u l and w l , update the output value out of the PID controller corresponding to the flow l : out l =q l *e+u l *i+w l *d

[0100] E6. According to the output value out l , adjust the output power of the exhaust fan so that the flow rate value at the air inlet is within the preset flow rate range, and then return to step E1 after the preset time;

[0101] E7. Clear the flow error value e, and then return to step E1 after a preset time.

[0102] Since the pollen intelligent monitoring and analysis system of this scheme is placed outdoors for pollen collection, the outdoor air is affected by airflow and wind speed, which will affect the gas flow entering the pollen sampling chamber 41, thereby affecting the collected pollen concentration; in order to avoid interference from the external environment, this scheme collects the intake air flow to determine whether the airflow entering the pollen sampling chamber 41 is unstable, and adjusts the output power of the exhaust fan to ensure that the flow rate of the incoming air is within a certain range.

[0103] Since the types of plants that bloom in different seasons vary, the concentration of pollen in the air also varies greatly. For example, in spring, there are many flowering plants and the concentration of pollen in the air is high. If the collection time is relatively long, the pollen on the slide 8 will overlap, affecting the accurate identification of the pollen concentration or quantity.

[0104] In this regard, the control method of the pollen intelligent monitoring and analysis system of this solution also includes a method for preventing pollen sampling from stacking on the glass slide 8. The method S includes steps S1 to S8.

[0105] This solution divides the entire sampling area on the slide 8 into 30 small sampling areas. During the anti-stacking process, a virtual coordinate system will be established for the slide 8, and the coordinate information of each sampling area will be recorded when scanning and photographing. After the first sampling, a sampling result image will be obtained. The sampling area to which the image belongs can be determined based on the X-axis information of the image coordinates. For example, the X-axis range between 46-50 is sampling area 1, and the X-axis range between 41-45 is sampling area 2. The calculation formula for the minimum X-axis range Xmin and the maximum X-axis range Xmax of any sampling area n is: Xmin = 46-5*n; Xmax = 50-5*n.

[0106] In this scheme, at the initial moment of sampling, the total sampling time of a single glass slide 8 is one day, and the initial value of the sampling residence time = 24h / N, where N is the total number of initial sampling areas; when pollen sampling is performed, the starting time of pollen sampling in each sampling area is counted, and when the timing time is equal to the corresponding sampling time, sampling of the sampling area is stopped.

[0107] After the first sampling and scanning, a picture of each sampling area can be obtained, and then the anti-stacking control method of this scheme can be used to adjust the sampling step size of the next slide 8 and the total number of sampling areas to avoid stacking or excessive dispersion of pollen in each sampling area.

[0108] In step S1 , multiple image information of each sampling area of ​​the current glass slide 8 is obtained, and the images are preprocessed to obtain grayscale images. The current glass slide is the most recent glass slide 8 on which pollen sampling has been completed.

[0109] This solution preferably collects 5 pictures in each sampling area. Specifically, each sampling area is divided into 5 equal parts, and one picture is collected in each equal part of each area.

[0110] In step S2, the grayscale value of each pixel in the grayscale image is calculated, and the count value of the pixel in the black area of ​​each sampling area is determined according to the grayscale value;

[0111] During implementation, the preferred step S2 of this solution further includes:

[0112] S21. Calculate the grayscale value Gray of each pixel in the grayscale image: Gray=0.299*R+0.587*G+0.114*B

[0113] Where R, G, and B are the values ​​of the red, green, and blue channels of each pixel in the grayscale image, respectively;

[0114] S22, setting the pixel points whose grayscale value is less than the grayscale threshold to zero, and setting the pixel points whose grayscale value is greater than or equal to the grayscale threshold to 255;

[0115] S23. Count the total number of zero pixels in the same grayscale image, and calculate the average value of the total number of pixels in all grayscale images in the same sampling area as the count value.

[0116] In step S3, it is determined whether all sampling ratios of the current slide 8 are greater than 1 or whether the number of sampling areas of the current slide 8 is less than the total number of initial sampling areas. If so, the process proceeds to step S6; otherwise, the process proceeds to step S4.

[0117] In step S4, the output out1 of the PID controller is updated according to the count value of each sampling area, and the sampling ratio Pr is updated = sampling ratio Pr + out1;

[0118] In one embodiment of the present invention, the method for updating the output out1 of the PID controller includes:

[0119] S41. Calculate the error value Errvalue, the error cumulative value Intvalue, and the error change rate Dervalue in the PID controller according to the count value of the sampling area: Errvalue = Setvalue - Curvalue Intvalue = Intvalue0 + Errvalue Dervalue = Errvalue - Preverror

[0120] Among them, Setvalue is the preset threshold; Curvalue is the count value; Preverror is the error correction value; Intvalue0 is the error accumulation value of the sampling area corresponding to the previous slide;

[0121] During implementation, the preferred method for obtaining the error correction value of this solution includes:

[0122] B1. Determine whether the error values ​​of at least two slides 8 have been recorded before the current slide 8 is collected in the current month; if so, proceed to step B2; otherwise, proceed to step B4;

[0123] B2. Calculate the average of the error values ​​of all slides 8 in the current month corresponding to the same sampling area of ​​the current slide 8;

[0124] B3, obtaining the error value of the sampling area corresponding to the previous slide 8 of the current sampling area of ​​the current slide 8, and calculating the average of the error value and the average value as the final error correction value;

[0125] B4. Use the error value of the previous glass slide 8 corresponding to the same sampling area of ​​the current glass slide 8 as the error correction value.

[0126] S42. Determine the proportional coefficient Kp, integral coefficient Ki, and differential coefficient Kd in the PID controller according to the error value and the preset threshold value:

[0127] When abs(Errvalue-Setvalue) <Th1:Kp=0,Ki=0,Kd=0;

[0128] When Th1 <abs(Errvalue-Setvalue)<Th2:Kp=Kp0,Ki=10*Ki0,Kd=Kd0;

[0129] When Th2 <abs(Errvalue-Setvalue):Kp=10*Kp0,Ki=Ki0,Kd=Kd0;

[0130] Where abs(.) is the absolute value function; Th1 and Th2 are the upper and lower thresholds, respectively; Kp0, Ki0, and Kd0 are the proportional coefficient, integral coefficient, and differential coefficient of the sampling area corresponding to the previous slide, respectively;

[0131] S43. Update the output out1 of the PID controller according to Errvalue, Intvalue, Dervalue, Kp, Ki, and Kd: Out1 = Kp*Errvalue+Ki*Intvalue+Kd*Dervalue.

[0132] In order to improve the future prediction performance of this solution, this solution preferably further includes between step S42 and step S43:

[0133] A1. Determine whether at least one glass slide 8 has been collected before the current glass slide 8 is collected. If so, proceed to step A2; otherwise, proceed directly to step S43.

[0134] A2. Increase the differential coefficient Kd obtained in step S42 by a preset multiple, and then proceed to step S43.

[0135] In step S5, when Pr≥1, the sampling time of the sampling area corresponding to the next slide 8 is equal to the sampling dwell time of the current slide 8; when Pr<1, the sampling time of the sampling area corresponding to the next slide 8 = Ts×Pr.

[0136] In step S6, the maximum value among the count values ​​of all sampling areas of the current slide 8 is selected as the stacking degree feedback value;

[0137] In step S7, the output out2 of the PID controller is updated according to the stacking degree feedback value, and the number of sampling areas St=St+int(out2) is updated, where int is a rounding function;

[0138] Methods for updating the output out2 of the PID controller include:

[0139] S71. Calculate the stacking error value Errvalue2, the stacking error cumulative value Intvalue2, and the stacking error change rate Dervalue2 in the PID controller according to the stacking degree feedback value of the current slide 8: Errvalue2 = Setvalue2 - Curvalue2 Intvalue2 = Intvalue20 + Errvalue2 Dervalue2 = Errvalue2 - Intvalue20

[0140] Among them, Curvalue2 is the stacking degree feedback value; Setvalue2 is the preset parameter value; Intvalue20 is the stacking error value corresponding to the last update of the sampling dwell time;

[0141] S72. Update the stack proportional coefficient Kp2, the stack differential coefficient Ki2, and the stack integral coefficient Kd2:

[0142] When Curvalue2 <Thmin:Kp2=10*Kp2,Ki2=Ki2,Kd2=0;

[0143] When Thmin≤Curvalue2 <Thmax:Kp2=0,Ki2=Ki2,Kd2=0;

[0144] When Thmax≤Curvalue2: Kp2=10*Kp2, Ki2=Ki2, Kd2=0;

[0145] Among them, Kp20 and Ki20 are the stacking proportional coefficient and stacking differential coefficient corresponding to the last updated sampling dwell time; Thmin and Thmax are the lower and upper limits of the stacking degree threshold respectively;

[0146] S73. Calculate the output out2 of the PID controller: Out2 = Kp2*Errvalue2+Ki2*Intvalue2+Kd2*Dervalue2.

[0147] In step S8, based on the total sampling time Cy of a single glass slide 8 and the number of sampling areas St, the sampling dwell time Ts=Cy / St of the sampling area of ​​the next glass slide 8 is updated, and the sampling time of each sampling area is equal to Ts.

[0148] This solution determines the number of pixels within the black area of ​​the sampling region based on the grayscale values ​​of the pixels in the image of the sampling region. Based on the conditions set in step S3, the sampling ratio of each sampling region is adjusted in conjunction with the output of the PID controller if the conditions are not met. This prevents overlapping pollen collected within the sampling regions. When the conditions are met, the number of sampling regions is adjusted to avoid excessive pollen dispersion due to too many sampling regions, or excessive pollen overlap due to too few sampling regions.

[0149] This solution analyzes the overlap and dispersion of pollen on the slide 8 that has recently completed pollen collection, and adjusts the sampling residence time or the number of sampling areas in each sampling area to avoid the situation where pollen is stacked or too dispersed again when the next slide 8 is used for pollen sampling, affecting the accurate statistics of the pollen number, thereby ensuring the accuracy of subsequent pollen number statistics and pollen contour identification.

[0150] After sampling of a glass slide 8 is completed, this solution executes steps S1 to S8 once. The entire method includes several steps S1 to S8, for example, the algorithm is terminated once after executing 30 times. After that, when the control method of this solution is started, all parameters are initialized.

[0151] In summary, the pollen intelligent monitoring and analysis system and its control method can realize the accurate collection of pollen concentration. It can also prevent interference from the external environment during the collection process. At the same time, when the pollen concentration in the air is high, pollen will pile up.

Claims

1. Pollen intelligent monitoring and analysis system, including a monitoring box, characterized in that: The monitoring box is provided with a control module and a support platform, on which are mounted a slide storage rack for storing a plurality of non-overlapping slides, a pollen sampling assembly for inhaling external ambient air and collecting pollen in the air onto the slides inside the monitoring box by volumetric method, an image acquisition device for amplifying the pollen image on the slides and then acquiring the image, and a slide transport mechanism for grabbing the slides on the slide storage rack and moving them to the pollen sampling assembly and the image acquisition device; The slide storage rack is mounted on a rotating mechanism, and the image acquisition device is provided with a fine-tuning mechanism for adjusting the slide in the X, Y, and Z directions. The rotating mechanism, pollen sampling assembly, image acquisition device, slide transport mechanism, and fine-tuning mechanism are all connected to a control module, which is configured to control the start and stop of components connected thereto, identify images uploaded by the image acquisition device, obtain pollen concentration and / or pollen count, and transmit the images, concentration, and / or pollen count to an external management terminal. The control module includes a micro host and an electrical box connected to each other. The rotating mechanism, pollen sampling assembly, image acquisition device, slide transport mechanism, and fine-tuning mechanism are all connected to the electrical box and a backup power supply. The micro host is embedded with an execution program for controlling the rotating mechanism, pollen sampling assembly, image acquisition device, slide transport mechanism, and fine-tuning mechanism. The micro host is connected to an input module placed in the monitoring box, and the input module is used to adjust the embedded execution program. A display electrically connected to the micro host is fixed to the inner side of the door panel of the monitoring box. The monitoring box is also provided with a temperature sensor, a humidity sensor, an air conditioner for keeping the internal temperature of the monitoring box constant at a preset temperature, and a dehumidifier for dehumidifying when the internal humidity of the monitoring box is greater than the preset humidity. The temperature sensor, humidity sensor, air conditioner and dehumidifier are all electrically connected to the control module; The control method of the pollen intelligent monitoring and analysis system includes the following steps: C1. Determine whether the pollen sampling assembly has completed pollen collection on at least one glass slide. If so, proceed to step C2; otherwise, proceed to step C4. C2. Read the number of sampling areas on the glass slide in the pollen sampling assembly and the sampling time of the last sampling area, and calculate the difference between the cut-off sampling time of the last sampling area and the cut-off time of the total sampling time; C3. When the difference is equal to zero, open the pollen sampling assembly, move the glass slide with pollen collected to the position where it was initially stored on the glass slide storage rack, and then proceed to step C4. When the difference is greater than zero, proceed to step C4; C4. At the sampling start time of the slide to be sampled, the serial number of the slide to be sampled is read, and the rotating mechanism is started to rotate the slide with the corresponding serial number on the slide storage rack to the grabbing side of the slide transfer mechanism; C5. Start the slide transport mechanism to grab the slide with the corresponding serial number and move it into the open pollen sampling assembly, and then close the pollen sampling assembly; C6. The pollen sampling component moves the sampling area on the glass slide to align with the air inlet according to the number of sampling areas on the glass slide and the sampling time of each sampling area, and draws air to hit the corresponding area on the glass slide for pollen sampling.

2. The pollen intelligent monitoring and analysis system according to claim 1, characterized in that: The pollen sampling assembly includes a base plate and a pollen sampling chamber formed by the engagement of a glass cover and a buffer plate. The base plate is mounted with a vertically extending vertical plate. The glass cover is fixed to the vertical plate. A linear guide rail that slides with the buffer plate is provided on the vertical plate below the glass cover. A through-type stepping motor that engages and disengages the buffer plate from the glass cover is mounted on the base plate. A glass slide placement table is installed on the inner surface of the buffer plate and slides relative to the buffer plate along the X-axis direction; a driving mechanism is fixed on the glass slide cover to slide the glass slide placement table; the top of the glass slide cover is connected to an air intake pipe extending from the monitoring box to introduce external air into and impact the glass slide, and an exhaust hole connected to the exhaust fan is installed on the side wall of the glass slide cover; the through-type stepping motor and the driving mechanism are both electrically connected to the control module.

3. The pollen intelligent monitoring and analysis system according to claim 2, characterized in that: The lower surface of the buffer plate is connected to a pressure plate through multiple optical axis screws, and the diameter of the hole on the pressure plate for the optical axis screw to pass through is larger than the diameter of the optical axis screw; the pressure plate is fixedly connected to the screw rod of the through-type stepping motor, and a spring is installed on the optical axis screw between the pressure plate and the buffer plate.

4. The pollen intelligent monitoring and analysis system according to claim 2, characterized in that: The driving mechanism includes a driving motor fixed to the outer surface of the side wall of the glass slide cover, the screw shaft of the driving motor passes through the sealing plate and penetrates into the glass slide cover, the screw shaft is fitted with a screw nut I, and the screw nut I has a limit plate extending vertically toward the buffer plate; a limit groove is provided on the side of the glass slide placement table, and when the buffer plate is buckled with the glass slide cover, the limit plate extends into the limit groove; the driving motor is electrically connected to the control module.

5. The pollen intelligent monitoring and analysis system according to claim 1, characterized in that: The slide transfer mechanism includes a gripper and a rotating table that drives the gripper to rotate 360°, a support block is fixed to the rotating table, a first lifting mechanism that drives the gripper to rise and fall in the vertical direction is installed on the support block, a lifting platform is provided on the first lifting mechanism, a first moving mechanism that drives the gripper to move linearly on a horizontal plane is installed on the lifting platform, and the rotating table, the first lifting mechanism and the first moving mechanism are all connected to the control module.

6. The pollen intelligent monitoring and analysis system according to claim 5, characterized in that: The gripper includes a plate-like body fixed on the first moving mechanism, and the non-mounting end of the plate-like body is forked and U-shaped; the two arms of the U-shape are forked into at least two extension strips for supporting the glass slide, and the outermost extension strip is provided with an L-shaped groove that contacts the edge of the glass slide; the non-outermost extension strip is provided with a receiving groove with a length equal to the maximum width of the glass slide, which is used to support the glass slide.

7. The pollen intelligent monitoring and analysis system according to claim 1, characterized in that: The image acquisition device includes a microscope without an eyepiece, a camera electrically connected to a control module is installed at the installation position of the eyepiece of the microscope, and the fine-tuning mechanism is installed on the mirror arm of the microscope; The fine adjustment mechanism includes a slide carrier, an L-shaped slide, and a second lifting mechanism fixed to the mirror arm for lifting the microscope stage along the Z axis. The short side of the slide is provided with a boss parallel to its long side, and the boss is located in a slide groove along the Y axis provided on the lower surface of the stage. The mounting plate of the long side of the slide extends upward from the side of the stage, and its top surface is located above the stage. The slide is fixed on the second moving mechanism on the mirror arm and slides in the slide groove through the second moving mechanism; a third moving mechanism is fixed on the side of the mounting plate facing the stage, which carries the glass slide carrier and slides along the X-axis direction.

8. The pollen intelligent monitoring and analysis system according to claim 7, characterized in that: The glass slide carrier includes an L-shaped slide, the short side of the slide is fixed on the screw nut II of the third moving mechanism, the long side of the slide extends along the length direction of the screw of the third moving mechanism, and a support plate with two cantilevers is fixed on it, and the cantilever is provided with a card slot for loading the glass slide.

9. The pollen intelligent monitoring and analysis system according to claim 1, characterized in that: The control method of the pollen intelligent monitoring and analysis system also includes: D1. Determine whether the last glass slide that has completed pollen collection is located on the gripping side of the glass slide transfer mechanism. If so, proceed to step D3; otherwise, proceed to step D2. D2. Start the rotating mechanism to rotate the slide storage rack, so that the previous slide with pollen collection completed is rotated to the grabbing side of the slide transfer mechanism; D3. Grab the last glass slide that has completed pollen collection and transfer it to the image acquisition device, and collect multiple images of each sampling area on the glass slide in turn; D4. When multiple images have been collected for each sampling area of ​​the slide, the slide is moved to the position where it was initially stored on the slide storage rack.

10. The pollen intelligent monitoring and analysis system according to claim 1, characterized in that: The control method of the pollen intelligent monitoring and analysis system also includes adaptively adjusting the air flow entering the pollen sampling component: E1. When the exhaust fan is delivering air to the pollen sampling assembly, collect the flow rate value at the air inlet of the pollen sampling assembly; E2. Determine whether the flow rate value is within the preset flow rate range. If so, proceed to step E7; otherwise, proceed to step E3. E3. Calculate the flow error value e, flow error cumulative value i and flow error change rate d in the PID controller based on the flow value: e=sf,i=i0+e,d=ep Where s is the preset flow threshold; f is the flow value; p is the last flow error value; i0 is the accumulated flow error value corresponding to the last flow judgment; E4. According to the flow error value, adjust the proportional coefficient q corresponding to the flow in the PID controller l , integral coefficient u l and the differential coefficient w l : When e<1, q l =q l0 ,u l =u l0 , w l =w l0 *0.5; when e≥1, q l =q l0 *2,u l =u l0 , w l =w l0 ; Among them, q l0 is the proportional coefficient threshold; u l0 is the integral coefficient threshold; w l0 is the differential coefficient threshold; E5. According to e, i, d and q l 、u l and w l , update the output value out of the PID controller corresponding to the flow l : out l =q l *e+u l *i+w l *d E6. According to the output value out l , adjust the output power of the exhaust fan so that the flow rate value at the air inlet is within the preset flow rate range, and then return to step E1 after the preset time; E7. Clear the flow error value e, and then return to step E1 after a preset time.

11. The pollen intelligent monitoring and analysis system according to claim 1, characterized in that: The control method of the pollen intelligent monitoring and analysis system also includes a method for preventing pollen sampling from stacking on a glass slide: S1. Obtain multiple image information of each sampling area of ​​the current slide and preprocess the image to obtain a grayscale image. The current slide is the most recent slide on which pollen sampling has been completed; S2, calculating the grayscale value of each pixel in the grayscale image, and determining the count value of the pixel in the black area of ​​each sampling area according to the grayscale value; S3, determining whether all sampling ratios of the current slide are greater than 1 or whether the number of sampling areas of the current slide is less than the total number of initial sampling areas, if so, proceeding to step S6, otherwise, proceeding to step S4; S4. Update the output out1 of the PID controller according to the count value of each sampling area, and update the sampling ratio Pr = sampling ratio Pr + out1; S5. When Pr≥1, the sampling time of the sampling area corresponding to the next slide is equal to the sampling dwell time of the current slide. When Pr<1, the sampling time of the sampling area corresponding to the next slide = Ts×Pr; S6. Select the maximum value among the count values ​​of all sampling areas of the current slide as the stacking degree feedback value; S7. Update the output out2 of the PID controller according to the stacking degree feedback value, and update the number of sampling areas St = St + int (out2), where int is a rounding function; S8. Update the sampling dwell time Ts=Cy / St of the next glass slide sampling area according to the total sampling time Cy of a single glass slide and the number of sampling areas St. The sampling dwell time of each sampling area is equal to Ts.

Images