Marker related to colorectal adenoma and use thereof

Abstract

Disclosed in the present invention are 45 markers related to colorectal adenoma, including one or more of TMPRSS15, ADGRE1, ADGRE5, AFM, APCS, BLNK, CABP2, CASP7, CCL25, CLIC5, COMP, ENPP2, F12, FASLG, FCER2, FCRL1, GAMT, GIP, HS3ST3B1, IGFBP1, IL4R, INPP1, ITPA, KAZN, KLK3, LPL, LSM1, LTA4H, MRPS16, NFASC, NOTCH3, NTF3, PAIP2B, PPY, PRAME, SERPING1, SOD3, SOST, SPINT3, STX7, SULT2A1, SWAP70, TEX101, YJU2 and ZCCHC8. By means of detecting the content, expression level and / or activity of the markers described above, the screening, diagnosis, monitoring, efficacy evaluation or prognosis evaluation of colorectal adenoma can be realized.

Description

Markers related to colorectal adenoma and their applications Technical Field

[0001] The present invention belongs to the field of diagnostic technology, and in particular relates to markers related to colorectal adenoma and applications thereof. Background Art

[0002] Intestinal polyps refer to the proliferation of the intestinal mucosal epithelium, forming polyp-like growths that protrude into the intestinal cavity. Patients with intestinal polyps may not have any clinical symptoms. Some may experience abdominal distension, abdominal pain, increased mucus in the stool, and severe cases may have blood in the stool. Intestinal polyps can be divided into neoplastic and non-neoplastic types based on their pathological manifestations. Neoplastic polyps are adenomatous polyps, and non-neoplastic polyps include hyperplastic polyps, inflammatory polyps, and hamartomatous polyps. Colorectal adenomas are benign glandular epithelial tumors originating from the intestinal epithelium. The interior can be composed of tubular, villous, or serrated structures. They are the most common precancerous lesions of colorectal cancer, accounting for 85%-90% of all precancerous lesions of colorectal cancer. Therefore, improving the detection rate of colorectal adenomas is extremely important for reducing the morbidity and mortality of colorectal cancer. Summary of the Invention

[0003] The purpose of the first aspect of the present invention is to provide the use of a substance for detecting a marker in the preparation of a product for screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas.

[0004] The second aspect of the present invention aims to provide a marker combination for screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas.

[0005] The third aspect of the present invention aims to provide a product for screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas.

[0006] The fourth aspect of the present invention aims to provide a method for constructing a model for screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas.

[0007] The fifth aspect of the present invention aims to provide a device or system for screening, diagnosing, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas.

[0008] The sixth aspect of the present invention aims to provide a computer device.

[0009] The seventh aspect of the present invention aims to provide a computer storable medium.

[0010] The eighth aspect of the present invention aims to provide a method for constructing a colorectal adenoma model.

[0011] The purpose of the ninth aspect of the present invention is to provide a method for screening, diagnosing, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas.

[0012] In order to achieve the above object, the technical solution adopted by the present invention is:

[0013] The first aspect of the present invention provides the use of a substance for detecting a marker in preparing a product:

[0014] The product is used for: screening, diagnosis, monitoring, efficacy evaluation, or prognosis assessment of colorectal adenomas;

[0015] The marker is A, and A comprises at least one of B, that is, it can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, or 45 of B;

[0016] The B is: ADGRE1, ADGRE5, AFM, APCS, BLNK, CABP2, CASP7, CCL25, CLIC5, COMP, ENPP2, F12, FASLG, FCER2, FCRL1, GAMT, GIP, HS3ST3B1, IGFBP1, IL4R, INPP1, ITPA, KAZN, KLK3, LPL, LSM1, LTA4H, MRPS16, NFASC, NOTCH3, NTF3, PAIP2B, PPY, PRAME, SERPING1, SOD3, SOST, SPINT3, STX7, SULT2A1, SWAP70, TEX101, TMPRSS15, YJU2, ZCCHC8.

[0017] Preferably, the A comprises any 1, 2, 3, 4, 5, 6, 7 or 8 of the Bs.

[0018] Preferably, the A comprises a single marker, and the single marker is any one of the B, such as ADGRE1, ADGRE5, AFM, APCS, BLNK, CABP2, CASP7, CCL25, CLIC5, COMP, ENPP2, F12, FASLG, FCER2, FCRL1, GAMT, GIP, HS3ST3B1, IGFBP1, IL4R, INPP1, ITPA, KAZN, KLK3, LPL, LSM1, LTA4H, MRPS16, NFASC, NOTCH3, NTF3, PAIP2B, PPY, PRAME, SERPING1, SOD3, SOST, SPINT3, STX7, SULT2A1, SWAP70, TEX101, TMPRSS15, YJU2, or ZCCHC8.

[0019] Preferably, the single marker is: SPINT3, TEX101, IGFBP1, STX7, APCS, KLK3, IL4R, SULT2A1, SERPING1, CLIC5, F12, LTA4H, SOST, CCL25, HS3ST3B1, FASLG, PPY, LPL, GIP, ADGRE1, TMPRSS15, FCRL1, COMP, LSM1, ENPP2, FCER2, NOTCH3, ADGRE5, CABP2, GAMT, BLNK, NTF3, CASP7, NFASC, or ITPA: wherein SPINT3, TEX101, IGFBP1, STX7, APCS, KLK3, IL4R, SULT2A1 , SERPING1, CLIC5, F12, LTA4H, SOST, CCL25, and HS3ST3B1 were increased relative to the reference level (corresponding to proteins with LogFC greater than 0 in Table 2), while the content, expression and / or activity of FASLG, PPY, LPL, GIP, ADGRE1, TMPRSS15, FCRL1, COMP, LSM1, ENPP2, FCER2, NOTCH3, ADGRE5, CABP2, GAMT, BLNK, NTF3, CASP7, NFASC, and ITPA were decreased relative to the reference level (corresponding to proteins with LogFC less than 0 in Table 2). The reference level represents the content, expression and / or activity of subjects of the same age group who do not have colorectal adenomas.

[0020] Preferably, the reference level represents the content, expression level and / or activity of healthy subjects of the same age group.

[0021] Preferably, the single marker is: TMPRSS15, IL4R, GIP, CASP7, PPY, SPINT3, FASLG, LPL, GAMT, FCRL1, or SULT2A1. Preferably, the single marker is: TMPRSS15, IL4R, GAMT, FCRL1, or SULT2A1. Preferably, the single marker is: TMPRSS15 or IL4R.

[0022] Preferably, the A includes a combination of 2 markers, and the combination of 2 markers is any 2 of the B.

[0023] Preferably, the two marker combinations are: IL4R and PPY; FCRL1 and GIP; GIP and IL4R; IL4R and TMPRSS15; GIP and ZCCHC8; INPP1 and TMPRSS15; IL4R and LPL; FASLG and GIP; GAMT and GIP; PPY and ZCCHC8; F12 and GIP; IL4R and SULT2A1; SULT2A1 and TMPRSS15; SWAP70 and TMPRSS15; GAMT and TMPRSS15; CLIC5 and SWAP70; GAMT and PPY; PPY and SULT2A1; CASP7 and TMPRSS15; IL4R and SWAP7 0;FASLG and TMPRSS15;CLIC5 and INPP1;PPY and SWAP70;PPY and SPINT3;GIP and HS3ST3B1;IGFBP1 and IL4R;IL4R and SOD3;CASP7 and GIP;CASP7 and GAMT;F12 and PPY;CCL25 and TMPRSS15;IL4R and NOTCH3;COMP and IL4R;INPP1 and PPY;SPINT3 and SWAP70;F12 and SWAP70;GIP and SULT2A1;FASLG and IL4R;IL4R and SPINT3;GIP and MRPS16;FASLG and SWAP70; CASP7 and CLIC5; FCRL1 and PPY; GIP and INPP1; ADGRE1 and GIP; FCRL1 and SWAP70; GIP and SPINT3; IL4R and INPP1; TMPRSS15 and ZCCHC8; GIP and SWAP70; ENPP2 and GIP; CASP7 and IL4R; ITPA and SPINT3; LSM1 and TMPRSS15; KLK3 and SWAP70; IL4R and NFASC; IGFBP1 and NTF3; CCL25 and PPY; LSM1 and PPY; GIP and TMPRSS15; GIP and KLK3; CCL25 and GIP; KAZN and KL K3; F12 and TMPRSS15; LTA4H and TMPRSS15; CASP7 and LPL; INPP1 and SOD3; FCRL1 and IGFBP1; KLK3 and PPY; GAMT and SPINT3; LTA4H and PPY; GAMT and IL4R; GIP and NFASC; INPP1 and STX7; APCS and GIP; BLNK and PPY; CASP7 and KLK3; STX7 and SWAP70; KLK3 and TMPRSS15; BLNK and GIP; KAZN and PPY; IGFBP1 and NFASC; IGFBP1 and INPP1; IL4R and STX7; FASLG and PPY;SPINT3 and TMPRSS15; FCRL1 and IL4R; ADGRE5 and APCS; KLK3 and LTA4H; FCRL1 and TMPRSS15; GIP and LSM1; NFASC and TMPRSS15; GIP and LTA4H; IGFBP1 and TMPRSS15; FASLG and LTA4H; GAMT and KLK3; or GIP and LPL.

[0024] Preferably, the A comprises a combination of 3 markers, and the combination of 3 markers is any 3 of the B.

[0025] Preferably, the three markers combination is: FCRL1, GIP and ZCCHC8; IL4R, INPP1 and PPY; IL4R, SULT2A1 and TMPRSS15; CLIC5, INPP1 and SOD3; F12, IL4R and PPY; FASLG, GIP and LTA4H; FASLG, GIP and IL4R; FCRL1, GIP and SWAP70; FASLG, GIP and ZCCHC8; ADGRE1, F12 and GIP; CCL25, FCRL1 and GIP; F12, PPY and SWAP70; GIP, SPINT3 and ZCCHC8; FCRL1, GIP and SULT2A1; IL4R , PPY and SULT2A1; CCL25, IL4R and PPY; IL4R, PPY and ZCCHC8; FCRL1, GIP and ITPA; F12, GIP and IL4R; F12, FCRL1 and GIP; FCRL1, GAMT and GIP; ADGRE1, GIP and ZCCHC8; CCL25, GIP and TMPRSS15; IL4R, NFASC and SULT2A1; IL4R, LPL and PPY; IL4R, INPP1 and TMPRSS15; FASLG, GIP and HS3ST3B1; CLIC5, INPP1 and TMPRSS15; CASP7, FCRL1 and GIP; G IP, IL4R and TMPRSS15; IL4R, PPY and SWAP70; FASLG, GIP and TMPRSS15; FASLG, FCRL1 and GIP; FASLG, GIP and SWAP70; COMP, IL4R and SPINT3; GIP, HS3ST3B1 and KLK3; FCRL1, GIP and KAZN; FASLG, SWAP70 and TMPRSS15; FCRL1, GIP and IL4R; FASLG, LTA4H and TMPRSS15; GIP, NFASC and SULT2A1; GIP, KLK3 and ZCCHC8; GIP, NTF3 and SULT2A1; KLK 3. LTA4H and PPY; FASLG, IL4R and PPY; CCL25, NFASC and PPY; GIP, IL4R and ZCCHC8; COMP, IL4R and SULT2A1; FCRL1, GIP and HS3ST3B1; LTA4H, NFASC and SULT2A1; FASLG, GIP and SULT2A1; F12, KLK3 and PPY; FASLG, LTA4H and PPY; FCRL1, GIP and SOST; IL4R, PPY and SOD3; CCL25, ENPP2 and GIP; CCL25, IL4R and TMPRSS15; ADGRE1, GIP and SULT2A1;GIP, IL4R, and SULT2A1; IL4R, NTF3, and SULT2A1; F12, GIP, and TMPRSS15; ADGRE5, FCRL1, and GIP; IL4R, NOTCH3, and SULT2A1; CCL25, FASLG, and TMPRSS15; F12, GIP, and SWAP70; CCL25, PPY, and SPINT3; CCL25, GIP, and NFASC; CCL25, NFASC, and TMPRSS15; CLIC5, INPP1, and SPINT3; FASLG, IL4R, and TMPRSS15; FCRL1, GIP, and STX7; PPY, SPINT3, and ZCCHC 8; CASP7, GIP and ZCCHC8; GIP, INPP1 and ZCCHC8; CLIC5, SWAP70 and TMPRSS15; COMP, IGFBP1 and IL4R; GIP, IL4R and KAZN; FASLG, GAMT and GIP; SULT2A1, SWAP70 and TMPRSS15; IL4R, SULT2A1 and SWAP70; BLNK, FCRL1 and GIP; CLIC5, INPP1 and LPL; IL4R, PPY and TMPRSS15; IL4R, PPY and SPINT3; GAMT, PPY and SULT2A1; GIP, NTF3 and ZCCHC8; LTA 4H, PPY and SULT2A1; ADGRE1, CCL25 and GIP; CLIC5, INPP1 and PPY; APCS, GIP and HS3ST3B1; IL4R, INPP1 and SPINT3; GIP, ITPA and SPINT3; ADGRE5, APCS and IL4R; GIP, LTA4H and ZCCHC8; IL4R, PPY and STX7; GIP, IL4R and SPINT3; FASLG, GIP and LSM1; PPY, SWAP70 and ZCCHC8; FCRL1, IGFBP1 and SWAP70; CASP7, CCL25 and GIP; BLNK, FASLG and GIP; A DGRE1, FASLG and GIP; F12, PPY and SULT2A1; FCER2, GIP and ZCCHC8; IL4R, KAZN and PPY; ADGRE5, GIP and ZCCHC8; IL4R, NFASC and PPY; INPP1, LPL and TMPRSS15; IL4R, SPINT3 and SWAP70; GIP, IL4R and INPP1; ADGRE1, FCRL1 and GIP; IL4R, INPP1 and SULT2A1; IL4R, SWAP70 and TMPRSS15; SERPING1, SWAP70 and TMPRSS15; KLK3, LTA4H and SWAP70;CASP7, F12 and GIP; IGFBP1, IL4R and NOTCH3; FASLG, IGFBP1 and SWAP70; CLIC5, PPY and SPINT3; GIP, KAZN and ZCCHC8; CASP7, FASLG and GIP; GIP, NOTCH3 and ZCCHC8; INPP1, PPY and SPINT3; F12, GAMT and GIP; FCRL1, GIP and IGFBP1; IL4R, STX7 and TMPRSS15; FCRL1, GIP and INPP1; NFASC, PPY and SULT2A1; ADGRE5, APCS and IGFBP1; APCS, COM P and IL4R; IL4R, KAZN and TMPRSS15; INPP1, PPY and SULT2A1; INPP1, PPY and ZCCHC8; APCS, FCRL1 and GIP; IGFBP1, NTF3 and SULT2A1; GAMT, IL4R and PPY; GIP, TMPRSS15 and ZCCHC8; FCRL1, GIP and SOD3; LTA4H, SULT2A1 and TMPRSS15; ADGRE5, IL4R and SULT2A1; CASP7, CLIC5 and INPP1; LTA4H, PPY and SPINT3; CLIC5, IL4R and INPP1; CLIC5, CO MP and SPINT3; FASLG, GIP and KAZN; KLK3, PPY and ZCCHC8; CABP2, FCRL1 and GIP; IL4R, KLK3 and PPY; CASP7, GIP and KLK3; ENPP2, GIP and ZCCHC8; CLIC5, FCRL1 and GIP; CLIC5, ITPA and SPINT3; CCL25, GIP and NOTCH3; PPY, SULT2A1 and ZCCHC8; GIP, TEX101 and ZCCHC8; IL4R, LTA4H and SULT2A1; PPY, SULT2A1 and SWAP70; CCL25, LPL and PPY; KLK3 , PPY and SWAP70; F12, SWAP70 and TMPRSS15; CASP7, FASLG and TMPRSS15; FASLG, GIP and SOD3; IL4R, INPP1 and LPL; GAMT, GIP and INPP1; IL4R, LTA4H and PPY; INPP1, LSM1 and PPY; FCRL1, GIP and NFASC; ADGRE1, GIP and IL4R; GAMT, SULT2A1 and TMPRSS15; CASP7, GIP and SPINT3; COMP, FASLG and IL4R; FCRL1, GIP and LSM1; GIP, HS3ST3B1 and NTF3;CLIC5, GAMT and INPP1; CCL25, GIP and IL4R; ADGRE1, GIP and HS3ST3B1; CCL25, PPY and TMPRSS15; CLIC5, COMP and IL4R; COMP, FCER2 and IL4R; CCL25, GIP and SPINT3; GIP, IL4R and LPL; F12, FASLG and GIP; FCRL1, GIP and LPL; GIP, LPL and ZCCHC8; GAMT, PPY and SWAP70; GAMT, IL4R and SWAP70; ADGRE5, F12 and GIP; GIP, SWAP70 and ZCCHC8; GIP, K AZN and KLK3; FASLG, LTA4H and SWAP70; FASLG, GIP and ITPA; GAMT, GIP and SULT2A1; CCL25, FCRL1 and PPY; FASLG, FCER2 and GIP; CLIC5, IGFBP1 and SWAP70; CLIC5, FASLG and SWAP70; IGFBP1, IL4R and PPY; COMP, IL4R and TMPRSS15; KLK3, SWAP70 and TMPRSS15; F12, GIP and ZCCHC8; GIP, SULT2A1 and SWAP70; CLIC5, FCRL1 and SWAP70; CASP7, G AMT and TMPRSS15; KLK3, STX7 and SWAP70; SPINT3, SWAP70 and TMPRSS15; FCRL1, IGFBP1 and PPY; F12, GIP and KLK3; F12, GIP and MRPS16; ENPP2, IL4R and PPY; F12, FCRL1 and SWAP70; CASP7, IL4R and TMPRSS15; INPP1, SULT2A1 and TMPRSS15; IGFBP1, INPP1 and PPY; FCRL1, GIP and TMPRSS15; GAMT, IL4R and TMPRSS15; CLIC5, INPP1 and STX7; CC L25, FASLG and GIP; GIP, IL4R and ITPA; PPY, STX7 and SWAP70; F12, PPY and SPINT3; ADGRE5, IL4R and PPY; GIP, IL4R and KLK3; FASLG, IL4R and SWAP70; IGFBP1, IL4R and SULT2A1; ADGRE5, GIP and SULT2A1; FCRL1, GIP and LTA4H; CABP2, GIP and TMPRSS15; F12, GIP and SOD3; COMP, IL4R and PPY; CLIC5, SPINT3 and TMPRSS15; CLIC5, COMP and INPP1;GAMT, GIP and ZCCHC8; IL4R, SPINT3 and TMPRSS15; GIP, HS3ST3B1 and ZCCHC8; FASLG, GIP and STX7; F12, IGFBP1 and SWAP70; APCS, IL4R and PPY; IL4R, PPY and TEX101; ENPP2, FCRL1 and GIP; ADGRE5, GIP and IL4R; INPP1, LTA4H and TMPRSS15; F12, IL4R and SWAP70; CLIC5, FASLG and GIP; GIP, KLK3 and SWAP70; GIP, PPY and ZCCHC8; ADGRE5, C CL25 and GIP; CCL25, STX7 and TMPRSS15; IGFBP1, NTF3 and SWAP70; CLIC5, IGFBP1 and INPP1; STX7, SWAP70 and TMPRSS15; CASP7, CLIC5 and SPINT3; GIP, IL4R and NOTCH3; CASP7, IL4R and SULT2A1; IL4R, STX7 and SWAP70; CCL25, KLK3 and PPY; ADGRE1, GIP and SPINT3; IGFBP1, IL4R and NTF3; LTA4H, SPINT3 and SWAP70; GIP, IGFBP1 and ZCCHC 8; IL4R, NOTCH3 and PPY; FASLG, LSM1 and PPY; CCL25, INPP1 and PPY; IGFBP1, PPY and ZCCHC8; F12, GIP and SPINT3; CASP7, GAMT and SULT2A1; CABP2, SWAP70 and TMPRSS15; FCRL1, GIP and MRPS16; FCRL1, PPY and SOST; COMP, GIP and IL4R; GIP, INPP1 and LTA4H; F12, FASLG and PPY; IL4R, INPP1 and STX7; GIP, KAZN and LTA4H; ENPP2, GIP and HS3ST 3B1; BLNK, F12 and PPY; LSM1, NFASC and SULT2A1; IL4R, SOD3 and TMPRSS15; F12, GIP and KAZN; ITPA, SPINT3 and SWAP70; GIP, IL4R and PPY; IL4R, SERPING1 and TMPRSS15; IGFBP1, IL4R and SWAP70; HS3ST3B1, IL4R and PPY; GIP, ITPA and SULT2A1; GIP, ITPA and ZCCHC8; LPL, SWAP70 and TMPRSS15; GIP, SOD3 and ZCCHC8; INPP1, LTA4H and PPY;FASLG, SULT2A1 and TMPRSS15; FASLG, SERPING1 and TMPRSS15; FASLG, LSM1 and TMPRSS15; CCL25, INPP1 and TMPRSS15; FASLG, PPY and ZCCHC8; CCL25, LPL and TMPRSS15; CASP7, LTA4H and SULT2A1; CASP7, GAMT and GIP; IL4R, LPL and SWAP70; CLIC5, IL4R and PPY; IGFBP1, SWAP70 and TMPRSS15; CASP7, SWAP70 and TMPRSS15; CASP7, GIP and IL4R F12, LPL and PPY; GIP, NFASC and ZCCHC8; F12, GIP and HS3ST3B1; GAMT, GIP and HS3ST3B1; CASP7, INPP1 and TMPRSS15; ADGRE5, APCS and GIP; F12, SPINT3 and SWAP70; APCS, SWAP70 and TMPRSS15; F12, IGFBP1 and PPY; PPY, SWAP70 and TEX101; PPY, SOST and SWAP70; CLIC5, FCRL1 and INPP1; COMP, F12 and IL4R; BLNK, CCL25 and PPY; FCRL1, PPY and ZCC HC8; CABP2, IL4R and SWAP70; GAMT, GIP and SPINT3; GAMT, PPY and SPINT3; CCL25, GIP and SWAP70; FCRL1, STX7 and SWAP70; HS3ST3B1, INPP1 and TMPRSS15; IL4R, LPL and SULT2A1; CABP2, GIP and HS3ST3B1; CLIC5, ITPA and SWAP70; GIP, IL4R and SWAP70; GIP, HS3ST3B1 and TMPRSS15; LPL, PPY and ZCCHC8; IL4R, ITPA and LPL; FASLG, GIP and NTF3; C ABP2, FASLG and GIP; GIP, STX7 and SWAP70; CCL25, ENPP2 and PPY; FCRL1, LTA4H and PPY; GIP, LTA4H and SULT2A1; FASLG, GAMT and TMPRSS15; F12, GIP and NTF3; CASP7, CCL25 and TMPRSS15; IGFBP1, IL4R and INPP1; GIP, KAZN and SPINT3; IGFBP1, INPP1 and TMPRSS15; FASLG, SOD3 and TMPRSS15; FASLG, INPP1 and TMPRSS15; CASP7, GIP and SULT2A1;FCRL1, GIP and NOTCH3; CABP2, COMP and IL4R; IL4R, LPL and TMPRSS15; GAMT, INPP1 and PPY; IGFBP1, NFASC and SULT2A1; GIP, SPINT3 and TMPRSS15; FASLG, GIP and SPINT3; CLIC5, FCRL1 and PPY; CLIC5, LPL and SWAP70; CASP7, IL4R and LPL; ADGRE5, F12 and PPY; GIP, IL4R and STX7; NTF3, SULT2A1 and SWAP70; GAMT, NFASC and SULT2A1; COMP, IL4R and LPL; LTA4H, SWAP70 and TMPRSS15; APCS, FASLG and GIP; CASP7, GIP and HS3ST3B1; GIP, HS3ST3B1 and SWAP70; CLIC5, F12 and SWAP70; CASP7, GIP and LTA4H; ADGRE5, FASLG and GIP; GAMT, IL4R and LPL; F12, FCER2 and GIP; IGFBP1, IL4R and LPL; APCS, GIP and IL4R; LTA4H, SULT2A1 and SWAP70; APCS, CLIC5 and GIP; F12, LSM1 and PPY; GAMT, GIP and IL 4R; GIP, LTA4H and TMPRSS15; F12, IGFBP1 and NTF3; F12, GIP and NFASC; LTA4H, SPINT3 and TMPRSS15; CLIC5, INPP1 and SWAP70; IL4R, INPP1 and SOD3; CLIC5, FASLG and TMPRSS15; CLIC5, GIP and SPINT3; IL4R, INPP1 and NOTCH3; APCS, F12 and GIP; CCL25, SWAP70 and TMPRSS15; NFASC, SULT2A1 and TMPRSS15; AFM, SWAP70 and TMPRSS15; IGFBP1 , LPL and NTF3; GAMT, GIP and TMPRSS15; HS3ST3B1, INPP1 and PPY; CABP2, IL4R and INPP1; GIP, SPINT3 and SWAP70; HS3ST3B1, IL4R and TMPRSS15; CLIC5, KLK3 and SWAP70; IL4R, INPP1 and KAZN; FCRL1, SWAP70 and TMPRSS15; IL4R, KLK3 and TMPRSS15; CASP7, GIP and INPP1; LTA4H, PPY and ZCCHC8; IL4R, ITPA and TMPRSS15; FASLG, GIP and MRPS16;PPY, SPINT3 and SWAP70; FASLG, GIP and KLK3; IL4R, ITPA and SPINT3; IL4R, LTA4H and SWAP70; COMP, IL4R and INPP1; F12, IL4R and TMPRSS15; FCRL1, PPY and SWAP70; CCL25, LSM1 and TMPRSS15; F12, FCRL1 and PPY; FCER2, IL4R and SULT2A1; GAMT, KLK3 and PPY; GIP, KLK3 and TMPRSS15; IL4R, LTA4H and TMPRSS15; GIP, KLK3 and LTA4H; FASLG, I NPP1 and PPY; CASP7, SPINT3 and TMPRSS15; GIP, HS3ST3B1 and SPINT3; F12, IGFBP1 and NFASC; IL4R, NOTCH3 and TMPRSS15; IL4R, LSM1 and TMPRSS15; CLIC5, SULT2A1 and SWAP70; FASLG, GIP and SERPING1; FASLG, IL4R and INPP1; CLIC5, GIP and ZCCHC8; FCRL1, GIP and PPY; INPP1, PPY and SWAP70; CLIC5, SPINT3 and SWAP70; APCS, GIP and SWAP 70; FCRL1, GIP and KLK3; IL4R, INPP1 and SWAP70; GIP, NOTCH3 and SULT2A1; F12, INPP1 and PPY; CLIC5, IL4R and NOTCH3; LTA4H, PPY and SWAP70; ADGRE1, GAMT and GIP; GIP, HS3ST3B1 and IL4R; CASP7, GIP and TMPRSS15; COMP, GAMT and IL4R; CLIC5, GIP and SWAP70; CLIC5, GIP and INPP1; INPP1, MRPS16 and TMPRSS15; GIP, SULT2A1 and TMPRSS1 5; FCRL1, GIP and SERPING1; F12, NFASC and PPY; ENPP2, F12 and GIP; FCRL1, IL4R and PPY; INPP1, SOST and TMPRSS15; INPP1, SWAP70 and TMPRSS15; GIP, INPP1 and STX7; FCRL1, IGFBP1 and SULT2A1; FCRL1, LTA4H and SWAP70; CLIC5, F12 and PPY; ADGRE5, APCS and GAMT; IGFBP1, PPY and SWAP70; CASP7, MRPS16 and SULT2A1; GIP, IL4R and NFASC;IGFBP1, IL4R and TMPRSS15; APCS, PPY and ZCCHC8; GIP, ITPA and KLK3; IGFBP1, SPINT3 and SWAP70; CLIC5, NFASC and SWAP70; CASP7, GAMT and KLK3; IL4R, NOTCH3 and STX7; FASLG, FCER2 and IL4R; ADGRE5, APCS and TMPRSS15; GIP, ITPA and SWAP70; GAMT, INPP1 and STX7; GAMT, STX7 and TMPRSS15; INPP1, KLK3 and PPY; GIP, HS3ST3B1 and INPP1; F1 2. GIP and LSM1; IGFBP1, IL4R and NFASC; F12, INPP1 and TMPRSS15; CCL25, IL4R and LPL; GAMT, PPY and ZCCHC8; MRPS16, PPY and SPINT3; IL4R, ITPA and SULT2A1; CLIC5, FCER2 and SWAP70; CASP7, CLIC5 and FCRL1; FASLG, SERPING1 and SWAP70; GAMT, SPINT3 and TMPRSS15; COMP, FCRL1 and IGFBP1; F12, IGFBP1 and SOD3; ADGRE5, GIP and HS3ST3B1; F CER2, GIP and HS3ST3B1; CABP2, PPY and SWAP70; FCRL1, PPY and SULT2A1; CCL25, LTA4H and PPY; CASP7, FASLG and LTA4H; CLIC5, INPP1 and ZCCHC8; F12, IL4R and NOTCH3; FASLG, ITPA and TMPRSS15; FASLG, IGFBP1 and IL4R; CLIC5, INPP1 and NOTCH3; ADGRE5, APCS and LSM1; CASP7, CLIC5 and GIP; GIP, LSM1 and ZCCHC8; CABP2, GIP and SWAP70; FASL G, IL4R and ITPA; CLIC5, F12 and GIP; IL4R, KLK3 and SWAP70; CLIC5, PPY and SWAP70; FASLG, IL4R and LPL; GIP, IGFBP1 and IL4R; GIP, IGFBP1 and SWAP70; PPY, SWAP70 and TMPRSS15; FCER2, IL4R and SWAP70; CCL25, LSM1 and PPY; CLIC5, PPY and ZCCHC8; CCL25, F12 and GIP; GAMT, IL4R and INPP1; SWAP70, TMPRSS15 and ZCCHC8; GIP, INPP1 and SULT2A1;GAMT, ITPA and SPINT3; APCS, PPY and SWAP70; LSM1, SULT2A1 and TMPRSS15; ADGRE5, F12 and TMPRSS15; ADGRE1, IL4R and LPL; BLNK, CLIC5 and INPP1; IL4R, PPY and SERPING1; BLNK, F12 and GIP; CCL25, GAMT and GIP; CASP7, GIP and SWAP70; FCER2, SWAP70 and TMPRSS15; F12, NTF3 and SWAP70; F12, NTF3 and PPY; CASP7, LTA4H and PPY; ADGRE5, F12 and IL4R; IL4R, KAZN and SULT2A1; F12, FASLG and TMPRSS15; CASP7, PPY and SPINT3; CASP7, CLIC5 and NOTCH3; FASLG, IL4R and SULT2A1; ADGRE1, GIP and STX7; IL4R, SOST and TMPRSS15; IL4R, MRPS16 and SULT2A1; GIP, HS3ST3B1 and LSM1; IL4R, NFASC and TMPRSS15; SPINT3, STX7 and SWAP70; APCS, FCRL1 and PPY; NFASC, PPY and SPINT3; CLIC5, G P and HS3ST3B1; FCRL1, LTA4H and SULT2A1; CASP7, GAMT and LTA4H; GIP, SWAP70 and TMPRSS15; GIP, HS3ST3B1 and NFASC; NTF3, PPY and ZCCHC8; IL4R, LPL and LTA4H; INPP1, LTA4H and STX7; BLNK, KLK3 and PPY; GAMT, GIP and ITPA; GIP, HS3ST3B1 and ITPA; INPP1, PPY and STX7; INPP1, LTA4H and SOD3; ADGRE5, GIP and SERPING1; ENPP2, GIP and KAZN; F ASLG, GIP and NOTCH3; ENPP2, GIP and IL4R; CLIC5, FASLG and SOD3; NFASC, SERPING1 and SULT2A1; HS3ST3B1, LSM1 and TMPRSS15; GIP, PPY and SPINT3; FASLG, HS3ST3B1 and TMPRSS15; ADGRE5, FASLG and IL4R; COMP, IL4R and STX7; FASLG, PPY and SWAP70; CASP7, GIP and MRPS16; INPP1, LSM1 and STX7; CASP7, IL4R and SPINT3; FASLG, GIP and INPP1;CCL25, GIP, and STX7; CCL25, KLK3, and TMPRSS15; GAMT, IL4R, and SULT2A1; CABP2, IL4R, and PPY; CASP7, GAMT, and SPINT3; CASP7, CLIC5, and COMP; FCRL1, ITPA, and SWAP70; INPP1, TMPRSS15, and ZCCHC8; IGFBP1, IL4R, and SOD3; GIP, STX7, and ZCCHC8; CCL25, GIP, and ZCCHC8; APCS, IL4R, and SWAP70; CLIC5, NFASC, and SULT2A1; FASLG, LSM1, and SWAP70 ; CLIC5, TMPRSS15 and ZCCHC8; FCRL1, GAMT and PPY; FASLG, GIP and IGFBP1; KAZN, SPINT3 and TMPRSS15; ADGRE5, FCRL1 and IGFBP1; CASP7, GIP and KAZN; GIP, SERPING1 and ZCCHC8; CABP2, GIP and ZCCHC8; CASP7, CCL25 and LPL; FASLG, IGFBP1 and TMPRSS15; IGFBP1, STX7 and SWAP70; F12, KLK3 and SWAP70; LTA4H, NTF3 and SULT2A1; CCL25, N OTCH3 and PPY; CASP7, CLIC5 and SWAP70; CLIC5, SOD3 and SWAP70; FASLG, KAZN and TMPRSS15; FCER2, PPY and ZCCHC8; APCS, IGFBP1 and SWAP70; GIP, INPP1 and SOD3; ADGRE5, FASLG and TMPRSS15; AFM, FCRL1 and GIP; FASLG, LSM1 and SOD3; INPP1, SOD3 and TMPRSS15; GIP, LTA4H and SWAP70; APCS, GAMT and GIP; CCL25, PPY and SWAP70; CLIC5, IL4 R and SPINT3; APCS, CCL25 and PPY; IL4R, KAZN and SWAP70; CLIC5, PPY and SULT2A1; CCL25, GIP and KLK3; FCER2, IL4R and PPY; APCS, IL4R and NOTCH3; CABP2, F12 and GIP; BLNK, CASP7 and CLIC5; GAMT, SWAP70 and TMPRSS15; INPP1, LSM1 and TMPRSS15; CLIC5, NTF3 and SWAP70; IGFBP1, ITPA and LPL; FCRL1, GIP and SPINT3; GIP, MRPS16 and SULT2A1;CASP7, GAMT, and LPL; IL4R, TMPRSS15, and ZCCHC8; GIP, SULT2A1, and ZCCHC8; HS3ST3B1, LSM1, and PPY; NTF3, SPINT3, and SWAP70; IL4R, ITPA, and PPY; GIP, MRPS16, and ZCCHC8; CASP7, PPY, and ZCCHC8; CASP7, GIP, and LPL; GIP, HS3ST3B1, and NOTCH3; LSM1, LTA4H, and PPY; CLIC5, IL4R, and SWAP70; GIP, LSM1, and SULT2A1; CABP2, IL4R, and TMPRSS15 ; CASP7, IL4R and PPY; ADGRE1, GIP and KLK3; F12, GIP and SULT2A1; GAMT, GIP and KLK3; GIP, INPP1 and TMPRSS15; CASP7, SULT2A1 and TMPRSS15; GIP, LTA4H and SPINT3; GIP, SOST and ZCCHC8; STX7, SULT2A1 and SWAP70; FCER2, GIP and IL4R; CASP7, ENPP2 and GIP; GAMT, LTA4H and TMPRSS15; CLIC5, GAMT and SWAP70; or IL4R, NTF3 and SWAP70. ;

[0026] Preferably, the A comprises a combination of 4 markers, and the combination of 4 markers is any 4 of the B. Preferably, the combination of 4 markers is: IL4R, PPY, SULT2A1 and NFASC; GIP, F12, HS3ST3B1 and NTF3; GIP, CCL25, ADGRE5 and APCS; IL4R, PPY, CCL25 and LPL; PPY, FCRL1, CCL25 and NFASC; GIP, IL4R, ADGRE5 and APCS; GIP, FCRL1, ZCCHC8 and F12; GIP, TMPRSS15, FASLG and CCL25; GIP, FCRL1, F12 and ITPA; PPY, ZCCHC8, CCL25 and NFASC; GIP, FCRL1 1. CCL25 and APCS; PPY, SPINT3, CCL25 and NFASC; GIP, ADGRE5, HS3ST3B1 and APCS; IL4R, PPY, CCL25 and APCS; GIP, TMPRSS15, FASLG and ZCCHC8; GIP, TMPRSS15, CCL25 and APCS; GIP, IL4R, SULT2A1 and NTF3; GIP, FCRL1, ZCCHC8 and SPINT3; GIP, CCL25, CASP7 and NFASC; GIP, SWAP70, FCRL1 and F12; GIP, PPY, ZCCHC8 and S PINT3; GIP, FCRL1, ZCCHC8, and CCL25; IL4R, PPY, CCL25, and NFASC; GIP, ZCCHC8, SPINT3, and ADGRE5; GIP, FASLG, ZCCHC8, and CCL25; GIP, FASLG, CCL25, and NFASC; GIP, CCL25, STX7, and ADGRE5; GIP, SULT2A1, FCRL1, and ZCCHC8; PPY, ZCCHC8, CCL25, and KLK3; GIP, FASLG, FCRL1, and ZCCHC8; GIP, TMPRSS15, CCL25, and CASP7 ;PPY, CCL25, STX7 and NFASC;TMPRSS15, FASLG, CCL25 and LTA4H;IL4R, PPY, SULT2A1 and NTF3;GIP, ZCCHC8, CCL25 and NFASC;GIP, FCRL1, ZCCHC8 and ADGRE5;GIP, FCRL1, CCL25 and CASP7;SULT2A1, IGFBP1, LTA4H and NFASC;PPY, CCL25, KLK3 and NFASC;GIP, ZCCHC8, SPINT3 and CCL25;GIP, FASLG, ZCCHC8 and ADGRE5;GIP, FCRL1, F12 and CASP7; IL4R, PPY, SPINT3 and F12; IL4R, PPY, SULT2A1 and F12; GIP, IL4R, SULT2A1 and NFASC; GIP, ZCCHC8, CCL25 and CASP7; GIP, ZCCHC8, SPINT3 and NTF3; IL4R, PPY, CCL25 and STX7; GIP, IL4R, FCRL1 and ZCCHC8; IL4R, IGFBP1, ADGRE5 and APCS; PPY, SULT2A1, CCL25 and NFASC; GIP, IL4R, CCL25 and NFASC; IL4R, PPY, ZCCHC8 and SPINT3; GIP, ZCCHC8, SPINT3 and ADGRE1; GIP, CCL25, CASP7 and APCS; IL4R, PPY, F12 and ADGRE5; GIP, ZCCHC8, HS3ST3B1 and ADGRE1; IL4R, IGFBP1, COMP and APCS; GIP, F12, CCL25 and NFASC; PPY, CCL25, NFASC and APCS; GIP, FCRL1, CCL25 and STX7; GIP, SULT2A1, LTA4H and NTF3; IL4R, PPY, FASLG and F12; GIP, SULT2A 1, FCRL1 and NFASC; IL4R, PPY, SULT2A1 and IGFBP1; IL4R, PPY, F12 and HS3ST3B1; GIP, FCRL1, CCL25 and NFASC; GIP, FASLG, ZCCHC8 and STX7; GIP, ZCCHC8, F12 and NTF3; GIP, F12, HS3ST3B1 and ADGRE1; GIP, CCL25, NFASC and APCS; PPY, ZCCHC8, SPINT3 and CCL25; GIP, FCRL1, F12 and ADGRE1; GIP, SWAP70, FASLG and ZCCHC8; GIP, FCRL 1, F12 and HS3ST3B1; GIP, CCL25, ADGRE1 and APCS; GIP, FASLG, ZCCHC8 and NTF3; GIP, PPY, ZCCHC8 and CCL25; GIP, FASLG, ZCCHC8 and HS3ST3B1; GIP, TMPRSS15, CCL25 and STX7; GIP, IL4R, FCRL1 and F12; GIP, SPINT3, CCL25 and CASP7; GIP, CCL25, HS3ST3B1 and APCS; TMPRSS15, SULT2A1, INPP1 and LTA4H; PPY, CCL25, ADGRE5 and APCS;IL4R, PPY, CCL25 and KLK3; GIP, PPY, FCRL1 and ZCCHC8; IL4R, PPY, SULT2A1 and COMP; GIP, SULT2A1, F12 and NTF3; GIP, ZCCHC8, CCL25 and ADGRE1; GIP, FCRL1, STX7 and ITPA; IL4R, PPY, F12 and GAMT; IL4R, PPY, SULT2A1 and ZCCHC8; GIP, HS3ST3B1, ADGRE1 and APCS; GIP, FCRL1, ZCCHC8 and STX7; GIP, SULT2A1, LTA4H and NFASC; GIP, INPP1, ZCCHC8 and SPINT3; GIP, IL4R, ZCCHC8 and SPINT3; GIP, SWAP70, FCRL1 and ZCCHC8; IL4R, PPY, SPINT3 and COMP; IL4R, TMPRSS15, SULT2A1 and COMP; GIP, ZCCHC8, HS3ST3B1 and NTF3; GIP, IL4R, F12 and KAZN; IL4R, PPY, CCL25 and ADGRE5; IL4R, PPY, INPP1 and CCL25; IL4R, PPY, SWAP70 and F12; GIP, FCRL1, F12 and GAMT; GIP, IN PP1, ZCCHC8 and LTA4H; TMPRSS15, FASLG, INPP1 and LTA4H; GIP, ZCCHC8, CCL25 and KLK3; GIP, FASLG, ZCCHC8 and CASP7; GIP, FCRL1, ZCCHC8 and CLIC5; IL4R, PPY, INPP1 and LTA4H; PPY, F12, CCL25 and NFASC; PPY, SULT2A1, LTA4H and NFASC; TMPRSS15, FASLG, SULT2A1 and LTA4H; INPP1, F12, CLIC5 and SOD3; TMPRSS15, CCL25, CASP 7 and LPL; IL4R, PPY, FASLG and CCL25; SULT2A1, IGFBP1, LTA4H and NTF3; IL4R, PPY, F12 and SOD3; INPP1, CLIC5, GAMT and SOD3; IL4R, PPY, ADGRE5 and APCS; PPY, CCL25, NFASC and KAZN; PPY, INPP1, ZCCHC8 and LTA4H; IL4R, PPY, LTA4H and STX7; PPY, TMPRSS15, FASLG and CCL25; IL4R, PPY, FCRL1 and CCL25; PPY, F12, HS3ST3B1 and NTF3;IL4R, PPY, INPP1 and STX7; PPY, ZCCHC8, SPINT3 and NFASC; PPY, TMPRSS15, CCL25 and NFASC; IL4R, PPY, SULT2A1 and INPP1; IL4R, SPINT3, CLIC5 and COMP; IL4R, PPY, INPP1 and SPINT3; SULT2A1, F12, IGFBP1 and NTF3; PPY, SULT2A1, F12 and NTF3; PPY, INPP1, ZCCHC8 and SPINT3; TMPRSS15, INPP1, F12 and CLIC5; IL4R, SPINT3, I FBP1 and COMP; TMPRSS15, SWAP70, FASLG and CLIC5; INPP1, CLIC5, COMP and SOD3; INPP1, SPINT3, CLIC5 and COMP; PPY, ZCCHC8, SPINT3 and CLIC5; PPY, SWAP70, F12 and CLIC5; INPP1, CLIC5, SOD3 and ITPA; PPY, INPP1, ZCCHC8 and CLIC5; PPY, INPP1, LTA4H and KLK3; PPY, SULT2A1, NFASC and NTF3; PPY, CCL25, NFASC and LPL; PPY, ZCC HC8, LTA4H, and KLK3; SWAP70, FCRL1, IGFBP1, and ITPA; TMPRSS15, SULT2A1, LTA4H, and NFASC; TMPRSS15, SWAP70, F12, and CLIC5; TMPRSS15, SWAP70, SULT2A1, and LTA4H; INPP1, SPINT3, CLIC5, and SOD3; PPY, CCL25, CASP7, and NFASC; SWAP70, SPINT3, IGFBP1, and STX7; PPY, F12, CCL25, and ADGRE5; TMPRSS15, SULT2A1, LTA4H, and GAMT ;SULT2A1, IGFBP1, NTF3 and ITPA; TMPRSS15, SWAP70, FASLG and LTA4H; SULT2A1, ZCCHC8, IGFBP1 and NTF3; INPP1, CLIC5, KLK3 and COMP; INPP1, CLIC5, LPL and HS3ST3B1; SPINT3, F12, CLIC5 and COMP; PPY, ZCCHC8, SPINT3 and NTF3; SWAP70, FCRL1, IGFBP1 and STX7; TMPRSS15, SWAP70, FASLG and ZCCHC8; IL4R, PPY, INPP1 and F12;IL4R, PPY, SWAP70 and STX7; TMPRSS15, INPP1, SPINT3 and CLIC5; SWAP70, IGFBP1, STX7 and ITPA; TMPRSS15, INPP1, CLIC5 and SOD3; TMPRSS15, CCL25, ADGRE5 and APCS; FCRL1, IGFBP1, STX7 and ITPA; TMPRSS15, FCRL1, CCL25 and STX7; TMPRSS15, SWAP70, CCL25 and STX7; TMPRSS15, SWAP70, FASLG and SULT2A1; TMPRSS15, SWAP7 0, FASLG and IGFBP1; TMPRSS15, SWAP70, CCL25 and LPL; SULT2A1, INPP1, IGFBP1 and LTA4H; SWAP70, CLIC5, IGFBP1 and STX7; INPP1, CLIC5, COMP and LPL; INPP1, CLIC5, LPL and SOD3; GIP, IL4R, CCL25 and STX7; GIP, TMPRSS15, FASLG and F12; GIP, TMPRSS15, CCL25 and KLK3; GIP, FCRL1, F12 and CCL25; GIP, FCRL1, ZCCHC8 and KAZN; GIP , FCRL1, F12 and CLIC5; GIP, FASLG, FCRL1 and F12; GIP, CCL25, CASP7 and STX7; GIP, FCRL1, ZCCHC8 and LPL; GIP, SWAP70, CCL25 and APCS; GIP, TMPRSS15, ZCCHC8 and CCL25; GIP, FCRL1, INPP1 and ZCCHC8; GIP, TMPRSS15, SPINT3 and CCL25; GIP, IL4R, HS3ST3B1 and APCS; GIP, NFASC, HS3ST3B1 and APCS; GIP, FCRL1, ZCCHC8 and CASP7; GIP, CCL25, CASP7 and KLK3; GIP, IL4R, SPINT3 and HS3ST3B1; GIP, ZCCHC8, SPINT3 and CASP7; GIP, HS3ST3B1, NTF3 and APCS; GIP, ZCCHC8, KLK3 and ADGRE1; GIP, IL4R, TMPRSS15 and FASLG; GIP, F12, CCL25 and ADGRE5; GIP, FASLG, CCL25 and CASP7; GIP, CCL25, CASP7 and LPL; GIP, FASLG, F12 and ADGRE1; GIP, FCRL1, ZCCHC8 and NFASC;GIP, TMPRSS15, CCL25 and NFASC; GIP, SULT2A1, GAMT and NTF3; GIP, SPINT3, CCL25 and ITPA; GIP, IL4R, SULT2A1 and ADGRE1; GIP, F12, ADGRE5 and HS3ST3B1; GIP, ZCCHC8, LTA4H and CASP7; IL4R, PPY, F12 and NFASC; GIP, FCRL1, F12 and ADGRE5; GIP, ZCCHC8, F12 and ADGRE1; GIP, ZCCHC8, CCL25 and NTF3; GIP, SULT2A1, F12 and NFASC; GIP , IL4R, STX7 and ADGRE5; GIP, IL4R, CCL25 and LPL; IL4R, PPY, TMPRSS15 and CCL25; GIP, F12, GAMT and NTF3; GIP, IL4R, FASLG and F12; GIP, SPINT3, CCL25 and NTF3; IL4R, PPY, SWAP70 and CCL25; GIP, IL4R, ADGRE5 and KAZN; GIP, ZCCHC8, STX7 and ADGRE1; GIP, SULT2A1, FCRL1 and F12; GIP, F12, CLIC5 and GAMT; GIP, SULT2A1, ZCCHC8 and NTF3 ; GIP, PPY, FASLG and ZCCHC8; GIP, F12, CCL25 and NTF3; GIP, ZCCHC8, ADGRE5 and APCS; GIP, IL4R, FASLG and ZCCHC8; GIP, FASLG, ZCCHC8 and SPINT3; GIP, FCRL1, ZCCHC8 and GAMT; GIP, FCRL1, ZCCHC8 and APCS; IL4R, PPY, SULT2A1 and GAMT; GIP, FASLG, INPP1 and LTA4H; GIP, FCRL1, CCL25 and ITPA; GIP, FASLG, CCL25 and KAZN; GIP, TMPRSS 15, CCL25 and NTF3; GIP, FCRL1, SPINT3 and CCL25; GIP, IL4R, FASLG and SOD3; GIP, IL4R, COMP and APCS; GIP, PPY, CCL25 and APCS; GIP, FASLG, CCL25 and GAMT; GIP, CCL25, NFASC and KAZN; GIP, IL4R, FASLG and ADGRE5; GIP, CCL25, STX7 and NFASC; GIP, IL4R, SWAP70 and STX7; GIP, TMPRSS15, CCL25 and ADGRE5; GIP, CCL25, NFASC and ADGRE1;IL4R, PPY, F12, and NTF3; GIP, FASLG, ZCCHC8, and LTA4H; GIP, FCRL1, ADGRE5, and APCS; GIP, IL4R, SULT2A1, and COMP; GIP, SWAP70, F12, and GAMT; GIP, TMPRSS15, SWAP70, and FASLG; GIP, IGFBP1, ADGRE5, and APCS; GIP, IL4R, FASLG, and GAMT; GIP, F12, GAMT, and HS3ST3B1; GIP, ZCCHC8, SPINT3, and HS3ST3B1; IL4R, TMPRSS15, SULT2A1, and LTA4 H; GIP, FASLG, HS3ST3B1 and APCS; GIP, F12, HS3ST3B1 and KAZN; GIP, ZCCHC8, SPINT3 and ITPA; GIP, FASLG, F12 and GAMT; GIP, PPY, SPINT3 and CCL25; IL4R, PPY, INPP1 and ZCCHC8; IL4R, PPY, ZCCHC8 and F12; GIP, FASLG, FCRL1 and CCL25; IL4R, PPY, F12 and COMP; GIP, FCRL1, ZCCHC8 and LTA4H; GIP, SWAP70, FASLG and LTA4H; IL4R, PPY, FA SLG and SULT2A1; GIP, IL4R, FASLG and KAZN; GIP, SPINT3, CCL25 and NFASC; GIP, FCRL1, ZCCHC8 and IGFBP1; IL4R, PPY, F12 and CLIC5; GIP, F12, CASP7 and HS3ST3B1; PPY, SPINT3, CCL25 and CASP7; GIP, GAMT, HS3ST3B1 and APCS; GIP, SWAP70, ZCCHC8 and SPINT3; GIP, F12, NFASC and HS3ST3B1; GIP, FCRL1, GAMT and STX7; GIP, FCRL1, APCS and ITPA; IL4R, SPINT3, CCL25 and COMP; GIP, CCL25, STX7 and ADGRE1; GIP, F12, GAMT and ADGRE5; IL4R, SPINT3, LTA4H and COMP; IL4R, PPY, CLIC5 and CCL25; GIP, FCRL1, F12 and KAZN; IL4R, TMPRSS15, CCL25 and LPL; IL4R, PPY, ZCCHC8 and CCL25; IL4R, SULT2A1, LTA4H and NFASC; IL4R, PPY, SPINT3 and IGFBP1; GIP, SULT2A1, NFASC and NTF3;GIP, FASLG, F12 and HS3ST3B1; GIP, FCRL1, CCL25 and ADGRE5; GIP, FCRL1, SPINT3 and ITPA; IL4R, PPY, CCL25 and COMP; GIP, TMPRSS15, F12 and CCL25; GIP, FCRL1, ZCCHC8 and ADGRE1; GIP, SULT2A1, FCRL1 and LTA4H; GIP, SWAP70, FCRL1 and APCS; IL4R, PPY, TMPRSS15 and SULT2A1; GIP, FCRL1, ZCCHC8 and KLK3; IL4R, PPY, SULT2A1 and APCS; GIP, ZCCHC8, CCL25 and ADGRE5; GIP, FCRL1, ZCCHC8 and NTF3; IL4R, CCL25, COMP and APCS; GIP, IL4R, FASLG and IGFBP1; GIP, F12, KLK3 and ITPA; GIP, SWAP70, FASLG and FCRL1; GIP, PPY, CCL25 and NFASC; GIP, IL4R, TMPRSS15 and F12; GIP, CCL25, KLK3 and NFASC; GIP, CCL25, LPL and ADGRE1; GIP, SWAP70, CCL25 and KLK3; GIP, IL 4R, SPINT3 and KAZN; GIP, IL4R, FCRL1 and COMP; IL4R, PPY, SULT2A1 and LTA4H; PPY, CLIC5, CCL25 and NFASC; IL4R, SULT2A1, IGFBP1 and NTF3; GIP, SWAP70, FCRL1 and STX7; IL4R, PPY, CCL25 and GAMT; GIP, ZCCHC8, NFASC and HS3ST3B1; GIP, CCL25, NFASC and HS3ST3B1; GIP, F12, NTF3 and ITPA; GIP, ZCCHC8, SPINT3 and NFASC; IL4R, P PY, F12 and LTA4H; GIP, IL4R, PPY and CCL25; GIP, SPINT3, CCL25 and KAZN; PPY, ZCCHC8, SPINT3 and LTA4H; GIP, SPINT3, CCL25 and STX7; GIP, FCRL1, F12 and STX7; GIP, FASLG, CASP7 and STX7; GIP, PPY, CCL25 and KLK3; PPY, ZCCHC8, SPINT3 and IGFBP1; GIP, CCL25, STX7 and ITPA; GIP, FASLG, ZCCHC8 and KLK3; GIP, FASLG, CCL25 and ITPA;IL4R, PPY, F12 and KLK3; GIP, FASLG, CCL25 and LTA4H; IL4R, TMPRSS15, CCL25 and COMP; GIP, FASLG, ZCCHC8 and NFASC; GIP, F12, ADGRE1 and ITPA; GIP, FASLG, SPINT3 and CASP7; IL4R, PPY, SULT2A1 and STX7; GIP, FASLG, F12 and CASP7; IL4R, PPY, F12 and ADGRE1; GIP, TMPRSS15, ZCCHC8 and SPINT3; GIP, CLIC5, ADGRE5 and APCS; GIP, IL4R , SPINT3 and CCL25; IL4R, PPY, INPP1 and KAZN; GIP, FASLG, CCL25 and NTF3; IL4R, SULT2A1, COMP and NTF3; IL4R, PPY, SPINT3 and CCL25; GIP, IL4R, NFASC and APCS; IL4R, PPY, SULT2A1 and HS3ST3B1; IL4R, SULT2A1, IGFBP1 and COMP; GIP, IL4R, FASLG and HS3ST3B1; GIP, CCL25, NTF3 and APCS; GIP, IL4R, F12 and ITPA; PPY, SPINT3, F12 and CC L25; GIP, ZCCHC8, CASP7 and KLK3; PPY, SULT2A1, INPP1 and LTA4H; IL4R, PPY, SULT2A1 and ADGRE5; GIP, FCRL1, CCL25 and LPL; GIP, SULT2A1, CCL25 and NFASC; IL4R, PPY, SPINT3 and KAZN; IL4R, PPY, SULT2A1 and CCL25; GIP, INPP1, SPINT3 and ITPA; IL4R, PPY, FASLG and INPP1; PPY, INPP1, SPINT3 and CLIC5; GIP, SWAP70, FASLG and STX7; GIP, CCL25, LTA4H and NFASC; GIP, FASLG, INPP1 and ZCCHC8; GIP, SWAP70, SPINT3 and CCL25; GIP, ZCCHC8, LTA4H and KLK3; IL4R, PPY, INPP1 and CLIC5; GIP, FCRL1, HS3ST3B1 and APCS; GIP, F12, GAMT and ITPA; GIP, FASLG, ZCCHC8 and ADGRE1; IL4R, TMPRSS15, SULT2A1 and NTF3; IL4R, PPY, SULT2A1 and KAZN; GIP, IGFBP1, CCL25 and NFASC;GIP, FASLG, CCL25 and APCS; PPY, SWAP70, SPINT3 and F12; GIP, IL4R, TMPRSS15 and SULT2A1; GIP, F12, GAMT and CASP7; GIP, FCRL1, ZCCHC8 and COMP; GIP, F12, CLIC5 and NFASC; IL4R, PPY, INPP1 and APCS; GIP, INPP1, LTA4H and CASP7; GIP, ZCCHC8, SPINT3 and GAMT; GIP, FCRL1, F12 and IGFBP1; GIP, HS3ST3B1, APCS and ITPA; GIP, SWAP7 0, KLK3 and STX7; GIP, ZCCHC8, CASP7 and HS3ST3B1; GIP, TMPRSS15, INPP1 and CCL25; IL4R, PPY, STX7 and ADGRE5; IL4R, TMPRSS15, FASLG and CCL25; GIP, FCRL1, F12 and LTA4H; GIP, FCRL1, IGFBP1 and ITPA; GIP, IL4R, LTA4H and KAZN; GIP, TMPRSS15, FCRL1 and CCL25; TMPRSS15, CCL25, STX7 and NFASC; IL4R, PPY, SPINT3 and LTA4H; GIP , F12, CLIC5 and KLK3; GIP, FASLG, FCRL1 and ITPA; GIP, IL4R, FASLG and CCL25; GIP, SPINT3, F12 and ITPA; IL4R, PPY, SWAP70 and FASLG; GIP, SWAP70, STX7 and ADGRE1; PPY, INPP1, CCL25 and KLK3; IL4R, PPY, F12 and IGFBP1; IL4R, PPY, SULT2A1 and ITPA; GIP, IL4R, CCL25 and APCS; IL4R, PPY, F12 and APCS; GIP, CCL25, NFASC and ITPA; GIP , FCRL1, INPP1 and CCL25; GIP, SPINT3, CCL25 and HS3ST3B1; GIP, IL4R, FCRL1 and STX7; GIP, SWAP70, FCRL1 and CCL25; IL4R, PPY, STX7 and KAZN; GIP, SWAP70, CASP7 and STX7; PPY, SPINT3, F12 and CLIC5; GIP, TMPRSS15, FCRL1 and ZCCHC8; SULT2A1, INPP1, LTA4H and CASP7; PPY, INPP1, ZCCHC8 and IGFBP1; GIP, IL4R, SULT2A1 and FCRL1;GIP, FCRL1, ZCCHC8 and HS3ST3B1; GIP, FASLG, ZCCHC8 and COMP; GIP, F12, CLIC5 and ITPA; IL4R, PPY, COMP and APCS; IL4R, PPY, CCL25 and HS3ST3B1; GIP, IL4R, F12 and HS3ST3B1; GIP, FASLG, GAMT and CASP7; GIP, F12, CCL25 and KAZN; IL4R, PPY, SULT2A1 and SPINT3; GIP, IL4R, F12 and ADGRE1; IL4R, PPY, F12 and KAZN; GIP, IL4R, F12 and ADGRE5; PPY, SWAP70, SPINT3 and CCL25; GIP, PPY, ZCCHC8 and LTA4H; GIP, IL4R, STX7 and ADGRE1; GIP, F12, KLK3 and HS3ST3B1; GIP, IL4R, ZCCHC8 and KAZN; IL4R, PPY, LTA4H and LPL; GIP, IL4R, SPINT3 and ITPA; IL4R, TMPRSS15, SULT2A1 and INPP1; GIP, FASLG, CCL25 and STX7; GIP, FCRL1, F12 and NTF3; PPY, ZCCHC8, CCL25 and LPL; GIP, F12, STX7 and ADGRE1; GIP, ZCCHC8, F12 and CASP7; GIP, IL4R, KLK3 and KAZN; PPY, ZCCHC8, ADGRE5 and APCS; GIP, IL4R, ZCCHC8 and ADGRE5; IL4R, PPY, STX7 and COMP; GIP, SULT2A1, FCRL1 and ITPA; GIP, SULT2A1, STX7 and NFASC; GIP, ZCCHC8, KLK3 and HS3ST3B1; GIP, CCL25, LTA4H and CASP7; IL4R, PPY, SPINT3 and HS3ST3B1; IL4 R, PPY, INPP1 and LPL; GIP, FASLG, LTA4H and CASP7; PPY, INPP1, SPINT3 and CCL25; INPP1, CLIC5, CASP7 and LPL; GIP, TMPRSS15, FASLG and CASP7; GIP, FCRL1, NFASC and HS3ST3B1; PPY, INPP1, CLIC5 and GAMT; IL4R, SULT2A1, COMP and APCS; GIP, CASP7, KLK3 and HS3ST3B1; IL4R, PPY, TMPRSS15 and F12; GIP, IL4R, SULT2A1 and ADGRE5;GIP, SWAP70, FASLG and HS3ST3B1; GIP, ZCCHC8, KLK3 and NTF3; GIP, SULT2A1, FCRL1 and GAMT; GIP, PPY, FCRL1 and F12; GIP, FASLG, CASP7 and HS3ST3B1; GIP, ZCCHC8, F12 and ADGRE5; IL4R, SULT2A1, SPINT3 and COMP; IL4R, TMPRSS15, CCL25 and ADGRE5; GIP, FCRL1, CCL25 and KAZN; GIP, GAMT, ADGRE5 and APCS; GIP, FCRL1, STX7 and HS3ST 3B1; SWAP70, SPINT3, IGFBP1 and LTA4H; PPY, SWAP70, F12 and KLK3; GIP, LTA4H, ADGRE5 and APCS; PPY, ZCCHC8, IGFBP1 and NTF3; GIP, CCL25, COMP and APCS; GIP, F12, CLIC5 and CASP7; PPY, INPP1, CCL25 and LTA4H; GIP, ZCCHC8, IGFBP1 and NTF3; GIP, CCL25, NFASC and LPL; GIP, F12, KAZN and ADGRE1; PPY, TMPRSS15, CCL25 and KLK3; GIP, I L4R, FASLG and SULT2A1; GIP, CASP7, HS3ST3B1 and APCS; GIP, F12, GAMT and ADGRE1; GIP, TMPRSS15, CCL25 and LPL; IL4R, PPY, ZCCHC8 and KLK3; GIP, F12, HS3ST3B1 and APCS; GIP, CCL25, HS3ST3B1 and NTF3; GIP, FCRL1, CCL25 and NTF3; GIP, IL4R, INPP1 and ZCCHC8; SWAP70, SPINT3, CLIC5 and ITPA; GIP, ZCCHC8, F12 and CLIC5; GIP, ZCCHC 8. CASP7 and ADGRE1; GIP, CCL25, KLK3 and ITPA; GIP, F12, HS3ST3B1 and ITPA; GIP, INPP1, ZCCHC8 and KLK3; GIP, SPINT3, CLIC5 and KAZN; IL4R, PPY, F12 and LPL; GIP, CCL25, GAMT and STX7; SWAP70, F12, IGFBP1 and NTF3; GIP, FCRL1, CLIC5 and CCL25; GIP, FASLG, ZCCHC8 and IGFBP1; SPINT3, CLIC5, GAMT and COMP; GIP, F12, LTA4H and CASP7;GIP, FCRL1, ZCCHC8 and ITPA; GIP, ZCCHC8, SPINT3 and F12; IL4R, SWAP70, IGFBP1 and APCS; IL4R, PPY, ZCCHC8 and LTA4H; GIP, FCRL1, INPP1 and F12; PPY, INPP1, CCL25 and STX7; PPY, SWAP70, F12 and IGFBP1; GIP, ZCCHC8, LTA4H and NFASC; GIP, FCRL1, CCL25 and COMP; IL4R, PPY, SWAP70 and SULT2A1; GIP, ZCCHC8, SPINT3 and LTA4H; GI P, PPY, ZCCHC8 and KLK3; GIP, ZCCHC8, LPL and ADGRE1; GIP, SPINT3, CLIC5 and ITPA; GIP, IL4R, F12 and GAMT; GIP, SWAP70, ZCCHC8 and CASP7; GIP, SWAP70, FASLG and F12; GIP, F12, CLIC5 and HS3ST3B1; GIP, SULT2A1, HS3ST3B1 and NTF3; IL4R, PPY, CCL25 and SOD3; GIP, ZCCHC8, CCL25 and COMP; PPY, SULT2A1, F12 and NFASC; GIP, STX7, HS3ST3B1 and ADGRE1; GIP, IL4R, FASLG and LTA4H; PPY, FASLG, INPP1 and LTA4H; GIP, SWAP70, FCRL1 and CLIC5; PPY, SWAP70, CCL25 and KLK3; IL4R, PPY, SPINT3 and KLK3; PPY, INPP1, F12 and LTA4H; GIP, F12, GAMT and NFASC; IL4R, SULT2A1, NFASC and ITPA; GIP, IL4R, HS3ST3B1 and ADGRE1; GIP, SPINT3, CASP7 and HS3ST3B1; GIP, GAMT , HS3ST3B1 and NTF3; GIP, ADGRE5, APCS and ITPA; GIP, F12, CLIC5 and ADGRE1; IL4R, PPY, IGFBP1 and STX7; GIP, F12, ADGRE5 and APCS; GIP, F12, CCL25 and ITPA; IL4R, PPY, STX7 and NFASC; GIP, ZCCHC8, CLIC5 and ADGRE1; GIP, ZCCHC8, LTA4H and NTF3; PPY, INPP1, LTA4H and HS3ST3B1; IL4R, PPY, CLIC5 and STX7; IL4R, PPY, INPP1 and GAMT;GIP, IL4R, FCRL1 and CCL25; GIP, IL4R, FASLG and COMP; IL4R, PPY, FASLG and ZCCHC8; GIP, TMPRSS15, F12 and GAMT; PPY, ZCCHC8, F12 and NTF3; PPY, SWAP70, ZCCHC8 and SPINT3; PPY, SULT2A1, ZCCHC8 and NTF3; PPY, FASLG, ZCCHC8 and LTA4H; GIP, CCL25, APCS and ITPA; PPY, ZCCHC8, KLK3 and NTF3; IL4R, IGFBP1, NTF3 and APCS; IL4R, P PY, CCL25 and LTA4H; GIP, IL4R, F12 and NTF3; GIP, F12, CLIC5 and SOD3; GIP, GAMT, KLK3 and HS3ST3B1; SWAP70, IGFBP1, KLK3 and STX7; GIP, TMPRSS15, SWAP70 and F12; GIP, FASLG, FCRL1 and CASP7; GIP, FASLG, ZCCHC8 and GAMT; IL4R, PPY, IGFBP1 and COMP; IL4R, PPY, SPINT3 and GAMT; IL4R, PPY, F12 and STX7; PPY, F12, GAMT and NTF3; IL 4R, TMPRSS15, SULT2A1 and NFASC; GIP, SWAP70, FCRL1 and IGFBP1; PPY, CCL25, CASP7 and KLK3; IL4R, SULT2A1, STX7 and NFASC; GIP, CCL25, GAMT and CASP7; PPY, SPINT3, F12 and NTF3; GIP, FASLG, F12 and SOD3; IL4R, PPY, FASLG and LTA4H; GIP, IL4R, ZCCHC8 and F12; GIP, IL4R, STX7 and ITPA; GIP, ZCCHC8, ADGRE1 and APCS; GIP, FASL G, ZCCHC8 and F12; GIP, INPP1, F12 and CLIC5; SWAP70, IGFBP1, LTA4H and KLK3; TMPRSS15, SULT2A1, IGFBP1 and NFASC; PPY, CCL25, NFASC and HS3ST3B1; IL4R, PPY, SULT2A1 and SOD3; IL4R, PPY, ZCCHC8 and NTF3; SWAP70, SPINT3, CLIC5 and STX7; GIP, FASLG, F12 and ITPA; PPY, SPINT3, F12 and LTA4H; IL4R, TMPRSS15, SULT2A1 and F12;GIP, SPINT3, F12 and ADGRE1; IGFBP1, STX7, ADGRE5 and APCS; GIP, FASLG, CCL25 and ADGRE5; GIP, SWAP70, SULT2A1 and FCRL1; GIP, SPINT3, F12 and HS3ST3B1; GIP, IL4R, FASLG and CASP7; GIP, CCL25, STX7 and NTF3; IL4R, PPY, F12 and CCL25; GIP, IL4R, CCL25 and KAZN; PPY, SULT2A1, CLIC5 and NFASC; GIP, SPINT3, CCL25 and ADGRE5; IL4R, PPY, IGFBP1 and LPL; PPY, ZCCHC8, CCL25 and NTF3; PPY, SULT2A1, F12 and LTA4H; GIP, FCRL1, F12 and SOD3; IL4R, SULT2A1, LTA4H and COMP; GIP, SWAP70, FCRL1 and ITPA; IL4R, SULT2A1, NFASC and NTF3; IL4R, TMPRSS15, SULT2A1 and HS3ST3B1; GIP, ZCCHC8, LTA4H and ADGRE5; GIP, SPINT3, F12 and GAMT; GIP, ZCCHC8, SPINT3 and KAZN; GIP, IL4R, INPP1 and GAMT; GIP, TMPRSS15, CCL25 and KAZN; GIP, IL4R, TMPRSS15 and CCL25; PPY, ZCCHC8, CLIC5 and NFASC; GIP, SWAP70, F12 and HS3ST3B1; GIP, FASLG, CCL25 and ADGRE1; PPY, ZCCHC8, CCL25 and APCS; IL4R, PPY, ZCCHC8 and ADGRE5; GIP, ZCCHC8, SPINT3 and STX7; GIP, IL4R, F12 and LPL; GIP, TMPRSS15, F12 and A DGRE1; GIP, IL4R, SWAP70 and SULT2A1; GIP, SWAP70, SPINT3 and STX7; IL4R, PPY, SPINT3 and NTF3; GIP, F12, CASP7 and ITPA; GIP, ZCCHC8, CCL25 and LPL; IL4R, TMPRSS15, CCL25 and NFASC; PPY, INPP1, GAMT and STX7; GIP, ZCCHC8, CASP7 and STX7; GIP, IL4R, SWAP70 and FASLG; GIP, INPP1, CCL25 and STX7; GIP, IL4R, CCL25 and ADGRE5;PPY, INPP1, CCL25 and NFASC; IL4R, PPY, IGFBP1 and APCS; GIP, FASLG, SULT2A1 and ZCCHC8; IL4R, TMPRSS15, SULT2A1 and IGFBP1; PPY, FCRL1, INPP1 and CLIC5; GIP, SWAP70, SPINT3 and F12; TMPRSS15, SWAP70, FASLG and STX7; PPY, TMPRSS15, CCL25 and APCS; PPY, SPINT3, CCL25 and STX7; IL4R, PPY, SWAP70 and ZCCHC8; PPY, INPP1 PP1, CCL25 and LPL; TMPRSS15, FASLG, CCL25 and CASP7; GIP, CASP7, HS3ST3B1 and NTF3; IL4R, PPY, SULT2A1 and ADGRE1; GIP, SWAP70, FASLG and CCL25; IL4R, PPY, CCL25 and KAZN; GIP, TMPRSS15, F12 and HS3ST3B1; GIP, SPINT3, HS3ST3B1 and NTF3; GIP, TMPRSS15, ZCCHC8 and CASP7; GIP, F12, CCL25 and HS3ST3B1; GIP, FASLG, FC RL1 and GAMT; GIP, INPP1, CCL25 and ADGRE1; GIP, ZCCHC8, KLK3 and ADGRE5; GIP, SULT2A1, LTA4H and GAMT; GIP, SULT2A1, ZCCHC8 and ADGRE1; GIP, SULT2A1, FCRL1 and CASP7; IL4R, PPY, ZCCHC8 and APCS; GIP, SPINT3, CCL25 and COMP; PPY, CCL25, GAMT and KLK3; GIP, PPY, F12 and HS3ST3B1; GIP, SULT2A1, INPP1 and LTA4H; GIP, FCRL 1. CCL25 and GAMT; GIP, IL4R, SWAP70 and APCS; GIP, IL4R, FCRL1 and ADGRE5; FCRL1, CCL25, ADGRE5 and APCS; GIP, PPY, CCL25 and STX7; GIP, TMPRSS15, FASLG and LTA4H; IL4R, IGFBP1, STX7 and ADGRE5; GIP, CCL25, ADGRE5 and KAZN; TMPRSS15, INPP1, CCL25 and LTA4H; GIP, TMPRSS15, CCL25 and SOD3; GIP, ZCCHC8, ADGRE1 and SOD3;GIP, TMPRSS15, FASLG, and SULT2A1; PPY, IGFBP1, ADGRE5, and APCS; GIP, IL4R, FASLG, and ADGRE1; GIP, IL4R, F12, and SOD3; GIP, ZCCHC8, CASP7, and NFASC; PPY, F12, GAMT, and HS3ST3B1; GIP, IL4R, PPY, and SULT2A1; IL4R, ADGRE5, and APCS and ITPA; GIP, CLIC5, HS3ST3B1, and APCS; GIP, ZCCHC8, CCL25, and STX7; GIP, SULT2A1, FCRL1, and STX 7; INPP1, ZCCHC8, CLIC5 and COMP; TMPRSS15, INPP1, CLIC5 and CCL25; GIP, CCL25, KLK3 and KAZN; GIP, SWAP70, F12 and NTF3; GIP, F12, GAMT and KLK3; GIP, INPP1, ZCCHC8 and CLIC5; GIP, FCRL1, F12 and NFASC; IL4R, INPP1, CLIC5 and COMP; PPY, INPP1, CLIC5 and CCL25; PPY, F12, CCL25 and KLK3; IL4R, TMPRSS15, INPP1 and LTA4H; GIP , SWAP70, FASLG and CLIC5; SWAP70, IGFBP1, LTA4H and STX7; PPY, FCRL1, INPP1 and CCL25; PPY, FASLG, CCL25 and NFASC; GIP, IL4R, SPINT3 and COMP; GIP, FASLG, ZCCHC8 and ITPA; SPINT3, CLIC5, COMP and ITPA; GIP, SWAP70, ZCCHC8 and KLK3; GIP, IL4R, SWAP70 and FCRL1; GIP, FCRL1, STX7 and KAZN; GIP, SWAP70, ZCCHC8 and ADGRE1; P PY, CCL25, STX7 and ADGRE5; PPY, SPINT3, CCL25 and HS3ST3B1; INPP1, CLIC5, GAMT and COMP; GIP, TMPRSS15, FCRL1 and F12; PPY, CCL25, KLK3 and KAZN; GIP, IL4R, LPL and HS3ST3B1; GIP, LTA4H, HS3ST3B1 and APCS; GIP, F12, CCL25 and ADGRE1; IL4R, TMPRSS15, SULT2A1 and GAMT; GIP, CCL25, CASP7 and NTF3; GIP, FCRL1, F12 and KLK3;GIP, FCRL1, SPINT3 and F12; IL4R, PPY, FCRL1 and F12; PPY, SWAP70, ZCCHC8 and CCL25; IL4R, SULT2A1, ADGRE5 and APCS; GIP, ZCCHC8, LTA4H and ADGRE1; SPINT3, F12, CLIC5 and ITPA; PPY, ZCCHC8, SPINT3 and HS3ST3B1; TMPRSS15, IGFBP1, CCL25 and NFASC; TMPRSS15, INPP1, CLIC5 and GAMT; PPY, SULT2A1, ZCCHC8 and NFASC; IL4R, CLIC 5. COMP and APCS; GIP, IGFBP1, NTF3 and APCS; TMPRSS15, SWAP70, SULT2A1 and FCRL1; GIP, FCRL1, F12 and LPL; PPY, INPP1, ZCCHC8 and CCL25; TMPRSS15, FASLG, INPP1 and CLIC5; GIP, CCL25, CASP7 and ADGRE1; GIP, CCL25, CASP7 and COMP; GIP, F12, CLIC5 and ADGRE5; GIP, SWAP70, STX7 and HS3ST3B1; IGFBP1, ADGRE5, APCS and ITPA; GIP, F CRL1, F12 and APCS; GIP, IL4R, FASLG and LPL; GIP, SPINT3, GAMT and ITPA; IL4R, PPY, FASLG and SPINT3; GIP, IL4R, FCRL1 and LPL; GIP, SULT2A1, ZCCHC8 and NFASC; GIP, F12, CCL25 and CASP7; GIP, FCRL1, STX7 and NTF3; PPY, SULT2A1, LTA4H and GAMT; GIP, SULT2A1, F12 and GAMT; GIP, SWAP70, HS3ST3B1 and APCS; IL4R, PPY, SPINT3 and STX7; GIP, IL4R, CCL25 and ADGRE1; GIP, ZCCHC8, CASP7 and NTF3; GIP, IL4R, FASLG and STX7; GAMT, ADGRE5, HS3ST3B1 and APCS; PPY, F12, CLIC5 and SOD3; IL4R, PPY, SPINT3 and ITPA; IGFBP1, ADGRE5, APCS and SOD3; GIP, ZCCHC8, KLK3 and COMP; GIP, GAMT, STX7 and ADGRE1; TMPRSS15, F12, CLIC5 and ADGRE5; GIP, TMPRSS15, SULT2A1 and ZCCHC8;TMPRSS15, CLIC5, CCL25, and NFASC; PPY, SWAP70, CCL25, and STX7; TMPRSS15, CCL25, STX7, and ADGRE5; GIP, STX7, ADGRE1, and ITPA; SWAP70, IGFBP1, LTA4H, and APCS; GIP, FCRL1, CASP7, and ITPA; PPY, ZCCHC8, HS3ST3B1, and NTF3; GIP, ZCCHC8, KLK3, and STX7; GIP, IL4R, PPY, and F12; IL4R, SULT2A1, GAMT, and NFASC; IL4R, F12, and IGFBP1 and COMP; SWAP70, F12, CLIC5 and IGFBP1; IL4R, F12, CLIC5 and COMP; GIP, F12, CASP7 and ADGRE1; INPP1, ZCCHC8, CLIC5 and LPL; IL4R, PPY, SPINT3 and ADGRE5; IL4R, PPY, CCL25 and NTF3; GIP, SWAP70, ZCCHC8 and CCL25; PPY, SWAP70, SPINT3 and LTA4H; GIP, FASLG, LTA4H and GAMT; GIP, SPINT3, STX7 and ITPA; IL4R, PPY, SULT2A1 and LP L; GIP, FCRL1, CLIC5 and APCS; PPY, TMPRSS15, ZCCHC8 and CCL25; GIP, FCRL1, GAMT and HS3ST3B1; GIP, FASLG, ZCCHC8 and SOD3; GIP, FASLG, CCL25 and SOD3; IL4R, PPY, CLIC5 and COMP; PPY, FCRL1, ZCCHC8 and F12; GIP, GAMT, STX7 and HS3ST3B1; GIP, FASLG, SULT2A1 and LTA4H; IL4R, CCL25, CASP7 and LPL; PPY, SPINT3, CCL25 and NTF 3; IL4R, SULT2A1, FCRL1 and COMP; GIP, FASLG, FCRL1 and KAZN; GIP, SWAP70, F12 and ADGRE1; GIP, PPY, ZCCHC8 and F12; PPY, INPP1, F12 and CLIC5; GIP, SPINT3, HS3ST3B1 and ITPA; SPINT3, CLIC5, STX7 and ITPA; PPY, IGFBP1, CCL25 and NFASC; IL4R, FASLG, IGFBP1 and SOD3; IL4R, PPY, SULT2A1 and FCRL1; GIP, FASLG, F12 and ADGRE5;IL4R, PPY, FASLG, and ADGRE5; PPY, FCRL1, F12, and CLIC5; INPP1, CLIC5, NFASC, and SOD3; IL4R, PPY, ZCCHC8, and LPL; IL4R, TMPRSS15, SPINT3, and COMP; GIP, FCRL1, F12, and COMP; TMPRSS15, INPP1, LTA4H, and CASP7; IL4R, SPINT3, CASP7, and COMP; GIP, F12, CLIC5, and KAZN; GIP, ZCCHC8, SPINT3, and COMP; GIP, TMPRSS15, SWAP70, and CCL25; PPY, CCL25, KLK3, and COMP; PPY, ZCCHC8, IGFBP1, and KLK3; or IL4R, TMPRSS15, SWAP70, and FASLG.

[0027] Preferably, the four markers are: IL4R, PPY, SULT2A1 and NFASC; GIP, F12, HS3ST3B1 and NTF3; GIP, CCL25, ADGRE5 and APCS; IL4R, PPY, CCL25 and LPL; PPY, FCRL1, CCL25 and NFASC; GIP, IL4R, ADGRE5 and APCS; GIP, FCRL1, ZCCHC8 and F12; GIP, TMPRSS15, FASLG and CCL25; GIP, FCRL1, F12 and ITPA; PPY, ZCCHC8, CCL25 and NFASC; GIP, FCRL1, CCL25 and AP CS; PPY, SPINT3, CCL25 and NFASC; GIP, ADGRE5, HS3ST3B1 and APCS; IL4R, PPY, CCL25 and APCS; GIP, TMPRSS15, FASLG and ZCCHC8; GIP, TMPRSS15, CCL25 and APCS; GIP, IL4R, SULT2A1 and NTF3; GIP, FCRL1, ZCCHC8 and SPINT3; GIP, CCL25, CASP7 and NFASC; GIP, SWAP70, FCRL1 and F12; GIP, PPY, ZCCHC8 and SPINT3; GIP, FCRL1, ZCCH C8 and CCL25; IL4R, PPY, CCL25 and NFASC; GIP, ZCCHC8, SPINT3 and ADGRE5; GIP, FASLG, ZCCHC8 and CCL25; GIP, FASLG, CCL25 and NFASC; GIP, CCL25, STX7 and ADGRE5; GIP, SULT2A1, FCRL1 and ZCCHC8; PPY, ZCCHC8, CCL25 and KLK3; GIP, FASLG, FCRL1 and ZCCHC8; GIP, TMPRSS15, CCL25 and CASP7; PPY, CCL25, STX7 and NFASC; TMPRSS15 , FASLG, CCL25 and LTA4H; IL4R, PPY, SULT2A1 and NTF3; GIP, ZCCHC8, CCL25 and NFASC; GIP, FCRL1, ZCCHC8 and ADGRE5; GIP, FCRL1, CCL25 and CASP7; SULT2A1, IGFBP1, LTA4H and NFASC; PPY, CCL25, KLK3 and NFASC; GIP, ZCCHC8, SPINT3 and CCL25; GIP, FASLG, ZCCHC8 and ADGRE5; GIP, FCRL1, F12 and CASP7; IL4R, PPY, SPINT3 and F12;IL4R, PPY, SULT2A1, and F12; GIP, IL4R, SULT2A1, and NFASC; GIP, ZCCHC8, CCL25, and CASP7; GIP, ZCCHC8, SPINT3, and NTF3; IL4R, PPY, CCL25, and STX7; GIP, IL4R, FCRL1, and ZCCHC8; IL4R, IGFBP1, ADGRE5, and APCS; PPY, SULT2A1, CCL25, and NFASC; GIP, IL4R, CCL25, and NFASC; IL4R, PPY, ZCCHC8, and SPINT3; GIP, ZCCHC8, SPINT3, and ADGRE5 E1; GIP, CCL25, CASP7 and APCS; IL4R, PPY, F12 and ADGRE5; GIP, ZCCHC8, HS3ST3B1 and ADGRE1; IL4R, IGFBP1, COMP and APCS; GIP, F12, CCL25 and NFASC; PPY, CCL25, NFASC and APCS; GIP, FCRL1, CCL25 and STX7; GIP, SULT2A1, LTA4H and NTF3; IL4R, PPY, FASLG and F12; GIP, SULT2A1, FCRL1 and NFASC; IL4R, PPY, SULT2A1 and IGFBP1; IL 4R, PPY, F12 and HS3ST3B1; GIP, FCRL1, CCL25 and NFASC; GIP, FASLG, ZCCHC8 and STX7; GIP, ZCCHC8, F12 and NTF3; GIP, F12, HS3ST3B1 and ADGRE1; GIP, CCL25, NFASC and APCS; PPY, ZCCHC8, SPINT3 and CCL25; GIP, FCRL1, F12 and ADGRE1; GIP, SWAP70, FASLG and ZCCHC8; GIP, FCRL1, F12 and HS3ST3B1; GIP, CCL25, ADGRE1 and APCS; GIP, FASLG, ZCCHC8 and NTF3; GIP, PPY, ZCCHC8 and CCL25; GIP, FASLG, ZCCHC8 and HS3ST3B1; GIP, TMPRSS15, CCL25 and STX7; GIP, IL4R, FCRL1 and F12; GIP, SPINT3, CCL25 and CASP7; GIP, CCL25, HS3ST3B1 and APCS; TMPRSS15, SULT2A1, INPP1 and LTA4H; PPY, CCL25, ADGRE5 and APCS; IL4R, PPY, CCL25 and KLK3; GIP, PPY, FCRL1 and ZCCHC8;IL4R, PPY, SULT2A1, and COMP; GIP, SULT2A1, F12, and NTF3; GIP, ZCCHC8, CCL25, and ADGRE1; GIP, FCRL1, STX7, and ITPA; IL4R, PPY, F12, and GAMT; IL4R, PPY, SULT2A1, and ZCCHC8; GIP, HS3ST3B1, ADGRE1, and APCS; GIP, FCRL1, ZCCHC8, and STX7; GIP, SULT2A1, LTA4H, and NFASC; GIP, INPP1, ZCCHC8, and SPINT3; GIP, IL4R, ZCCHC8, and SPINT3 ; GIP, SWAP70, FCRL1 and ZCCHC8; IL4R, PPY, SPINT3 and COMP; IL4R, TMPRSS15, SULT2A1 and COMP; GIP, ZCCHC8, HS3ST3B1 and NTF3; GIP, IL4R, F12 and KAZN; IL4R, PPY, CCL25 and ADGRE5; IL4R, PPY, INPP1 and CCL25; IL4R, PPY, SWAP70 and F12; GIP, FCRL1, F12 and GAMT; GIP, INPP1, ZCCHC8 and LTA4H; TMPRSS15, FASLG, INPP1 and LTA4H ; GIP, ZCCHC8, CCL25 and KLK3; GIP, FASLG, ZCCHC8 and CASP7; GIP, FCRL1, ZCCHC8 and CLIC5; IL4R, PPY, INPP1 and LTA4H; PPY, F12, CCL25 and NFASC; PPY, SULT2A1, LTA4H and NFASC; TMPRSS15, FASLG, SULT2A1 and LTA4H; INPP1, F12, CLIC5 and SOD3; TMPRSS15, CCL25, CASP7 and LPL; IL4R, PPY, FASLG and CCL25; SULT2A1, IGFBP1, L TA4H and NTF3; IL4R, PPY, F12 and SOD3; INPP1, CLIC5, GAMT and SOD3; IL4R, PPY, ADGRE5 and APCS; PPY, CCL25, NFASC and KAZN; PPY, INPP1, ZCCHC8 and LTA4H; IL4R, PPY, LTA4H and STX7; PPY, TMPRSS15, FASLG and CCL25; IL4R, PPY, FCRL1 and CCL25; PPY, F12, HS3ST3B1 and NTF3; IL4R, PPY, INPP1 and STX7; PPY, ZCCHC8, SPINT3 and NFASC;PPY, TMPRSS15, CCL25 and NFASC; IL4R, PPY, SULT2A1 and INPP1; IL4R, SPINT3, CLIC5 and COMP; IL4R, PPY, INPP1 and SPINT3; SULT2A1, F12, IGFBP1 and NTF3; PPY, SULT2A1, F12 and NTF3; PPY, INPP1, ZCCHC8 and SPINT3; TMPRSS15, INPP1, F12 and CLIC5; IL4R, SPINT3, IGFBP1 and COMP; TMPRSS15, SWAP70, FASLG and CLIC5; INPP1, C LIC5, COMP and SOD3; INPP1, SPINT3, CLIC5 and COMP; PPY, ZCCHC8, SPINT3 and CLIC5; PPY, SWAP70, F12 and CLIC5; INPP1, CLIC5, SOD3 and ITPA; PPY, INPP1, ZCCHC8 and CLIC5; PPY, INPP1, LTA4H and KLK3; PPY, SULT2A1, NFASC and NTF3; PPY, CCL25, NFASC and LPL; PPY, ZCCHC8, LTA4H and KLK3; SWAP70, FCRL1, IGFBP1 and ITPA; TMPRSS1 5. SULT2A1, LTA4H and NFASC; TMPRSS15, SWAP70, F12 and CLIC5; TMPRSS15, SWAP70, SULT2A1 and LTA4H; INPP1, SPINT3, CLIC5 and SOD3; PPY, CCL25, CASP7 and NFASC; SWAP70, SPINT3, IGFBP1 and STX7; PPY, F12, CCL25 and ADGRE5; TMPRSS15, SULT2A1, LTA4H and GAMT; SULT2A1, IGFBP1, NTF3 and ITPA; TMPRSS15, SWAP70, FASLG and LTA4H; SULT2A1, ZCCHC8, IGFBP1 and NTF3; INPP1, CLIC5, KLK3 and COMP; INPP1, CLIC5, LPL and HS3ST3B1; SPINT3, F12, CLIC5 and COMP; PPY, ZCCHC8, SPINT3 and NTF3; SWAP70, FCRL1, IGFBP1 and STX7; TMPRSS15, SWAP70, FASLG and ZCCHC8; IL4R, PPY, INPP1 and F12; IL4R, PPY, SWAP70 and STX7; TMPRSS15, INPP1, SPINT3 and CLIC5;SWAP70, IGFBP1, STX7 and ITPA; TMPRSS15, INPP1, CLIC5 and SOD3; TMPRSS15, CCL25, ADGRE5 and APCS; FCRL1, IGFBP1, STX7 and ITPA; TMPRSS15, FCRL1, CCL25 and STX7; TMPRSS15, SWAP70, CCL25 and STX7; TMPRSS15, SWAP70, FASLG and SULT2A1; TMPRSS15, SWAP70, FASLG and IGFBP1; TMPRSS15, SWAP70, CCL25 and LPL; SULT2A1, INPP1, IGFBP1 and LTA4H; SWAP70, CLIC5, IGFBP1 and STX7; INPP1, CLIC5, COMP and LPL; or INPP1, CLIC5, LPL and SOD3; that is, the above 185 4 Any one of the markers combination. ;

[0028] Preferably, the A comprises a combination of 5 markers, and the combination of 5 markers is any five of the combinations in B.

[0029] Preferably, the 5 markers are combined into a1) and a2):

[0030] a1) the 4 markers combination (preferably any one of the 185 4 markers combinations mentioned above);

[0031] a2) any one of the above B;

[0032] The a2) is different from any one marker in the combination of the four markers in a1).

[0033] Preferably, the five markers are in combination: IL4R, PPY, SULT2A1, F12, and NTF3; IL4R, PPY, SPINT3, COMP, and CLIC5; IL4R, PPY, SPINT3, COMP, and F12; IL4R, PPY, SPINT3, F12, and COMP; PPY, ZCCHC8, SPINT3, CCL25, and NFASC; PPY, ZCCHC8, SPINT3, NTF3, and F12; PPY, F12, HS3ST3B1, NTF3, and SERPING1; IL4R, PPY, SPINT3, COMP, and ITPA; IL4R, PPY, INPP1, LTA4 H and STX7; IL4R, SPINT3, CLIC5, COMP and PPY; GIP, F12, HS3ST3B1, ADGRE1 and NTF3; IL4R, PPY, SULT2A1, COMP and APCS; IL4R, PPY, CCL25, STX7 and ENPP2; GIP, F12, HS3ST3B1, NTF3 and ADGRE1; PPY, SPINT3, CCL25, NFASC and SERPING1; IL4R, PPY, SULT2A1, NTF3 and ZCCHC8; IL4R, PPY, SPINT3, COMP and SERPING1; IL4R, PPY, CC L25, STX7 and NOTCH3; IL4R, PPY, INPP1, LTA4H and ZCCHC8; SULT2A1, IGFBP1, NTF3, ITPA and F12; IL4R, PPY, SULT2A1, NTF3 and F12; PPY, CCL25, KLK3, NFASC and ZCCHC8; GIP, ADGRE5, HS3ST3B1, APCS and LSM1; PPY, CCL25, KLK3, NFASC and STX7; PPY, CCL25, STX7, NFASC and LSM1; PPY, SULT2A1, F12, NTF3 and IL4R; PPY, CCL25, KLK3 K3, NFASC and LSM1; PPY, SPINT3, CCL25, NFASC and ZCCHC8; IL4R, PPY, SPINT3, F12 and SWAP70; IL4R, PPY, SPINT3, COMP and CCL25; IL4R, PPY, SULT2A1, NTF3 and SERPING1; PPY, ZCCHC8, SPINT3, NTF3 and SERPING1; IL4R, PPY, SULT2A1, NFASC and CCL25; IL4R, IGFBP1, COMP, APCS and PPY; GIP, ADGRE5, HS3ST3B1, APCS and CCL25;PPY, SULT2A1, CCL25, NFASC and IL4R; IL4R, PPY, CCL25, NFASC and SULT2A1; IL4R, PPY, SPINT3, COMP and LTA4H; IL4R, PPY, CCL25, NFASC and SPINT3; PPY, SULT2A1, NFASC, NTF3 and IL4R; IL4R, PPY, SPINT3, COMP and STX7; IL4R, PPY, CCL25, STX7 and NFASC; SULT2A1, F12, IGFBP1, NTF3 and PPY; IL4R, PPY, LTA4H, STX7 and SULT2A1; GIP, ZCCHC8, F12, NTF3, and SPINT3; PPY, CCL25, STX7, NFASC, and IL4R; IL4R, PPY, SULT2A1, ZCCHC8, and NTF3; GIP, F12, HS3ST3B1, NTF3, and ZCCHC8; IL4R, PPY, SULT2A1, NTF3, and STX7; IL4R, PPY, SULT2A1, COMP, and F12; GIP, TMPRSS15, FASLG, CCL25, and ZCCHC8; IL4R, PPY, SULT2A1, NFASC, and F12; GIP, CCL25, ADGRE5, APCS, and HS3S T3B1; PPY, ZCCHC8, SPINT3, CLIC5 and COMP; PPY, F12, HS3ST3B1, NTF3 and SULT2A1; IL4R, PPY, SULT2A1, INPP1 and LTA4H; PPY, ZCCHC8, SPINT3, CLIC5 and NTF3; IL 4R, PPY, SPINT3, F12 and NTF3; PPY, INPP1, ZCCHC8, LTA4H and SPINT3; IL4R, PPY, SULT2A1, NTF3 and IGFBP1; PPY, SPINT3, CCL25, NFASC and LSM1; IL4R, PPY, INPP1, LTA4H and SULT2A1; IL4R, PPY, SPINT3, COMP and SOST; PPY, ZCCHC8, CCL25, NFASC and KLK3; GIP, TMPRSS15, CCL25, CASP7 and ENPP2; PPY, ZCCHC8, SPINT3, NFASC and CCL25; PPY, INPP1, LTA4H, KLK3 and ZCCHC8; TMPRSS15, SWAP70, CCL25, STX7 and KLK3; GIP, FASLG, ZCCHC8, CCL25 and ADGRE1; IL4R, PPY, SULT2A1, COMP and SERPING1;GIP, IL4R, SULT2A1, NTF3, and F12; IL4R, PPY, CCL25, STX7, and LPL; SWAP70, IGFBP1, STX7, ITPA, and SPINT3; IL4R, PPY, ADGRE5, APCS, and SERPING1; IL4R, SPINT3, CLIC5, COMP, and ITPA; GIP, HS3ST3B1, ADGRE1, APCS, and NTF3; GIP, ADGRE5, HS3ST3B1, APCS, and IL4R; IL4R, PPY, INPP1, STX7, and LTA4H; GIP, F12, HS3ST3B1, NTF3, and A PCS; PPY, CCL25, NFASC, APCS and CABP2; PPY, INPP1, ZCCHC8, LTA4H and KLK3; PPY, FCRL1, CCL25, NFASC and SERPING1; IL4R, PPY, SULT2A1, COMP and SPINT3; GIP, SULT2A1, F12, NTF3 and NFASC; SWAP70, SPINT3, IGFBP1, STX7 and ITPA; SPINT3, F12, CLIC5, COMP and PPY; PPY, FCRL1, CCL25, NFASC and LSM1; TMPRSS15, SULT2A1, IN PP1, LTA4H and IL4R; IL4R, PPY, SULT2A1, NTF3 and ITPA; IL4R, PPY, LTA4H, STX7 and NOTCH3; PPY, SWAP70, F12, CLIC5 and SPINT3; IL4R, PPY, F12, HS3ST3B1 and SULT2A1; GIP, CCL25, CASP7, NFASC and SPINT3; GIP, FCRL1, ZCCHC8, F12 and FASLG; IL4R, PPY, ZCCHC8, SPINT3 and COMP; SWAP70, CLIC5, IGFBP1, STX7 and KLK3; GIP, FCRL 1. CCL25, NFASC, and CABP2; IL4R, TMPRSS15, SULT2A1, COMP, and APCS; GIP, IL4R, ADGRE5, APCS, and HS3ST3B1; PPY, F12, CCL25, NFASC, and IL4R; GIP, ZCCHC8, CCL25, ADGRE1, and KLK3; TMPRSS15, CCL25, ADGRE5, APCS, and GIP; GIP, IL4R, ZCCHC8, SPINT3, and PPY; GIP, SPINT3, CCL25, CASP7, and ITPA; GIP, PPY, ZCCHC8, SPINT3, and NTF3;GIP, FASLG, FCRL1, ZCCHC8, and CCL25; TMPRSS15, SULT2A1, LTA4H, NFASC, and INPP1; INPP1, SPINT3, CLIC5, COMP, and IL4R; IL4R, PPY, FCRL1, CCL25, and SERPING1; PPY, ZCCHC8, LTA4H, KLK3, and INPP1; GIP, INPP1, ZCCHC8, LTA4H, and PPY; GIP, FASLG, CCL25, NFASC, and TMPRSS15; GIP, SWAP70, FCRL1, F12, and CCL25; PPY, TMPRSS1 5, CCL25, NFASC and SPINT3; TMPRSS15, SWAP70, FASLG, IGFBP1 and STX7; TMPRSS15, CCL25, CASP7, LPL and IL4R; PPY, TMPRSS15, FASLG, CCL25 and ZCCHC8; SWAP70, FCRL1, IGFBP1, STX7 and ITPA; SULT2A1, ZCCHC8, IGFBP1, NTF3 and PPY; GIP, CCL25, STX7, ADGRE5 and KLK3; GIP, FCRL1, F12, ADGRE1 and FASLG; GIP, PPY, FCRL1, ZC CHC8 and SPINT3; SULT2A1, INPP1, IGFBP1, LTA4H and TMPRSS15; TMPRSS15, FASLG, INPP1, LTA4H and SULT2A1; GIP, IL4R, CCL25, NFASC and KLK3; GIP, SULT2A1, FCRL1, ZCCHC8 and F12; GIP, F12, CCL25, NFASC and FCRL1; GIP, SWAP70, FASLG, ZCCHC8 and FCER2; FCRL1, IGFBP1, STX7, ITPA and SWAP70; TMPRSS15, FCRL1, CCL25, STX 7 and NOTCH3; TMPRSS15, SWAP70, SULT2A1, LTA4H and IL4R; GIP, FCRL1, STX7, ITPA and SWAP70; TMPRSS15, INPP1, SPINT3, CLIC5 and COMP; INPP1, CLIC5, SOD3, ITPA and SPINT3; INPP1, CLIC5, KLK3, COMP and IL4R; TMPRSS15, SWAP70, F12, CLIC5 and SPINT3; SULT2A1, IGFBP1, LTA4H, NTF3 and PPY; INPP1, F12, CLIC5, SOD3 and PPY;TMPRSS15, INPP1, CLIC5, SOD3, and LTA4H; GIP, IL4R, FCRL1, ZCCHC8, and FASLG; GIP, IL4R, F12, KAZN, and ADGRE1; INPP1, CLIC5, COMP, SOD3, and APCS; INPP1, CLIC5, LPL, HS3ST3B1, and ITPA; TMPRSS15, SWAP70, CCL25, STX7, and SPINT3; IL4R, PPY, SULT2A1, INPP1, and STX7; IL4R, PPY, SULT2A1, NFASC, and LSM1; GIP, ADGRE5, HS3ST3B 1. APCS and F12; SWAP70, SPINT3, IGFBP1, STX7 and PPY; PPY, SPINT3, CCL25, NFASC and F12; IL4R, PPY, SWAP70, STX7 and SERPING1; PPY, CCL25, STX7, NFASC and SERPING1; IL4R, PPY, CCL25, APCS and COMP; PPY, ZCCHC8, SPINT3, NTF3 and CLIC5; PPY, CCL25, STX7, NFASC and KLK3; IL4R, PPY, SWAP70, STX7 and IGFBP1; PPY, CCL25, CASP 7, NFASC and SPINT3; IL4R, PPY, LTA4H, STX7 and INPP1; PPY, CCL25, KLK3, NFASC and SERPING1; PPY, SULT2A1, F12, NTF3 and SPINT3; PPY, SULT2A1, F12, NTF3 and IGFBP1; PPY, SPINT3, CCL25, NFASC and IGFBP1; TMPRSS15, SULT2A1, INPP1, LTA4H and STX7; IL4R, IGFBP1, COMP, APCS and SOD3; IL4R, PPY, SPINT3, COMP and INPP1; PPY, Z CCHC8, SPINT3, NTF3 and CCL25; IL4R, PPY, CCL25, STX7 and SERPING1; IL4R, PPY, CCL25, NFASC and CABP2; IL4R, PPY, SULT2A1, F12 and LTA4H; GIP, SULT2A1, F12, NTF3 and ADGRE1; GIP, F12, HS3ST3B1, NTF3 and SULT2A1; GIP, F12, HS3ST3B1, ADGRE1 and NOTCH3; PPY, CCL25, NFASC, APCS and LSM1; PPY, ZCCHC8, SPINT3, NTF3 and IL4R;IL4R, PPY, SULT2A1, IGFBP1, and COMP; GIP, F12, HS3ST3B1, ADGRE1, and APCS; IL4R, SPINT3, CLIC5, COMP, and F12; IL4R, PPY, SULT2A1, F12, and COMP; GIP, F12, HS3ST3B1, NTF3, and ITPA; GIP, ZCCHC8, SPINT3, NTF3, and F12; IL4R, PPY, SPINT3, F12, and ADGRE5; PPY, INPP1, LTA4H, KLK3, and STX7; GIP, F12, HS3ST3B1, ADGRE1, and MRPS1 6; GIP, CCL25, HS3ST3B1, APCS and ADGRE5; PPY, SPINT3, CCL25, NFASC and MRPS16; SPINT3, F12, CLIC5, COMP and IL4R; GIP, F12, HS3ST3B1, NTF3 and LPL; IL4R, PPY, SULT2A1, COMP and ITPA; PPY, ZCCHC8, SPINT3, NTF3 and STX7; PPY, ZCCHC8, CCL25, NFASC and SPINT3; GIP, ADGRE5, HS3ST3B1, APCS and FASLG; SULT2A1, F12, IGFB P1, NTF3 and ITPA; GIP, TMPRSS15, FASLG, ZCCHC8 and CCL25; IL4R, PPY, INPP1, LTA4H and F12; IL4R, SPINT3, CLIC5, COMP and GIP; IL4R, PPY, SULT2A1, COMP and CABP2; IL4R, PPY, ZCCHC8, SPINT3 and INPP1; GIP, F12, HS3ST3B1, ADGRE1 and YJU2; GIP, F12, HS3ST3B1, NTF3 and ENPP2; GIP, SULT2A1, F12, NTF3 and HS3ST3B1; IL4R, PP Y, SPINT3, F12 and MRPS16; PPY, INPP1, ZCCHC8, SPINT3 and LTA4H; IL4R, SPINT3, CLIC5, COMP and SULT2A1; IL4R, PPY, FCRL1, CCL25 and NFASC; GIP, F12, HS3ST3B1, NTF3 and SPINT3; IL4R, PPY, SPINT3, COMP and MRPS16; IL4R, PPY, INPP1, LTA4H and CLIC5; PPY, F12, HS3ST3B1, NTF3 and IL4R; IL4R, SPINT3, CLIC5, COMP and LTA4H;GIP, ZCCHC8, CCL25, ADGRE1, and FASLG; IL4R, PPY, CCL25, NFASC, and APCS; IL4R, PPY, SULT2A1, NTF3, and NFASC; IL4R, PPY, SULT2A1, F12, and NFASC; GIP, TMPRSS15, FASLG, CCL25, and CASP7; IL4R, PPY, SULT2A1, NTF3, and LSM1; IL4R, PPY, CCL25, KLK3, and NFASC; IL4R, PPY, SULT2A1, NTF3, and APCS; IL4R, PPY, SPINT3, COMP, and APC S; IL4R, PPY, SULT2A1, COMP and LTA4H; IL4R, PPY, SULT2A1, COMP and ENPP2; GIP, CCL25, ADGRE5, APCS and TMPRSS15; GIP, HS3ST3B1, ADGRE1, APCS and F12; IL4R, PPY, SULT2A1, NTF3 and ADGRE5; IL4R, PPY, SPINT3, COMP and CABP2; IL4R, PPY, SULT2A1, NTF3 and CABP2; IL4R, PPY, SULT2A1, F12 and ADGRE1; PPY, CCL25, KLK3, N FASC and IL4R; GIP, CCL25, ADGRE5, APCS and NTF3; IL4R, PPY, SULT2A1, IGFBP1 and ITPA; IL4R, PPY, INPP1, SPINT3 and COMP; IL4R, PPY, SULT2A1, F12 and ITPA; GIP, FASLG, ZCCHC8, CCL25 and TMPRSS15; PPY, SULT2A1, LTA4H, NFASC and LSM1; IL4R, IGFBP1, COMP, APCS and SULT2A1; IL4R, PPY, SULT2A1, COMP and IGFBP1; GIP, F12 , HS3ST3B1, ADGRE1 and STX7; IL4R, PPY, INPP1, LTA4H and COMP; PPY, F12, HS3ST3B1, NTF3 and SPINT3; IL4R, PPY, SWAP70, STX7 and CLIC5; SPINT3, F12, CLIC5, COMP and GIP; GIP, ADGRE5, HS3ST3B1, APCS and LTA4H; GIP, ZCCHC8, SPINT3, NTF3 and CCL25; GIP, ZCCHC8, F12, NTF3 and KLK3; PPY, INPP1, ZCCHC8, LTA4H and TMPRSS15;PPY, SPINT3, CCL25, NFASC, and ITPA; IL4R, SPINT3, CLIC5, COMP, and TMPRSS15; PPY, CCL25, NFASC, APCS, and ZCCHC8; IL4R, SPINT3, CLIC5, COMP, and STX7; IL4R, SPINT3, CLIC5, COMP, and AFM; PPY, SULT2A1, F12, NTF3, and SERPING1; IL4R, IGFBP1, ADGRE5, APCS, and COMP; IL4R, PPY, CCL25, KLK3, and COMP; GIP, F12, HS3ST3B1, A DGRE1 and LSM1; IL4R, PPY, SPINT3, F12 and NOTCH3; PPY, CCL25, NFASC, APCS and IL4R; IL4R, PPY, F12, HS3ST3B1 and NTF3; PPY, SULT2A1, CCL25, NFASC and SERPING1; IL4R, PPY, ADGRE5, APCS and HS3ST3B1; IL4R, PPY, CCL25, NFASC and YJU2; IL4R, PPY, CCL25, NFASC and KLK3; IL4R, PPY, SULT2A1, NTF3 and BLNK; PPY, INPP1, ZCC HC8, LTA4H, and LSM1; IL4R, PPY, SULT2A1, NFASC, and SERPING1; IL4R, PPY, SULT2A1, NTF3, and LTA4H; GIP, ADGRE5, HS3ST3B1, APCS, and SPINT3; IL4R, PPY, SPINT3, COMP, and ADGRE5; GIP, ZCCHC8, SPINT3, ADGRE1, and F12; SULT2A1, F12, IGFBP1, NTF3, and TMPRSS15; IL4R, PPY, SULT2A1, F12, and HS3ST3B1; PPY, CCL25, KLK3, NFA SC and BLNK; GIP, ZCCHC8, SPINT3, CCL25 and NTF3; PPY, ZCCHC8, SPINT3, NTF3 and HS3ST3B1; IL4R, PPY, SULT2A1, INPP1 and ITPA; GIP, ADGRE5, HS3ST3B1, APCS and CABP2; GIP, ADGRE5, HS3ST3B1, APCS and NFASC; GIP, HS3ST3B1, ADGRE1, APCS and CCL25; IL4R, PPY, F12, HS3ST3B1 and ENPP2; IL4R, PPY, SWAP70, STX7 and SPINT3;PPY, INPP1, LTA4H, KLK3 and F12; IL4R, PPY, SWAP70, STX7 and SULT2A1; IL4R, SPINT3, IGFBP1, COMP and ITPA; IL4R, PPY, INPP1, LTA4H and NOTCH3; GIP, ADGRE5, HS3ST3B1, APCS and ZCCHC8; PPY, SULT2A1, CCL25, NFASC and SPINT3; IL4R, PPY, ADGRE5, APCS and COMP; GIP, HS3ST3B1, ADGRE1, APCS and ITPA; IL4R, PPY, SULT2A1, NT F3 and COMP; PPY, ZCCHC8, SPINT3, NFASC and F12; GIP, ZCCHC8, SPINT3, ADGRE1 and MRPS16; IL4R, PPY, SPINT3, COMP and SULT2A1; IL4R, PPY, CCL25, STX7 and SPINT3; PPY, ZCCHC8, SPINT3, NFASC and CLIC5; PPY, SULT2A1, F12, NTF3 and GAMT; IL4R, PPY, SULT2A1, NTF3 and ENPP2; IL4R, PPY, SULT2A1, ZCCHC8 and SPINT3; PPY, FCRL1, CCL25, NFASC and SPINT3; GIP, F12, HS3ST3B1, NTF3 and LSM1; GIP, F12, HS3ST3B1, NTF3 and CABP2; IL4R, PPY, SWAP70, STX7 and LTA4H; SPINT3, F12, CLIC5, COMP and TMPRSS15; IL4R, SPINT3, CLIC5, COMP and NTF3; IL4R, PPY, INPP1, LTA4H and ITPA; SPINT3, F12, CLIC5, COMP and ITPA; SPINT3, F12, CLIC5, COMP and CASP7; GIP, ZCCH C8, HS3ST3B1, NTF3 and F12; IL4R, PPY, SULT2A1, NFASC and ITPA; IL4R, PPY, SPINT3, COMP and AFM; IL4R, PPY, SULT2A1, NFASC and ZCCHC8; GIP, F12, HS3ST3B1, ADGRE1 and ZCCHC8; PPY, ZCCHC8, CCL25, NFASC and GIP; IL4R, PPY, SPINT3, F12 and ZCCHC8; GIP, HS3ST3B1, ADGRE1, APCS and ADGRE5; IL4R, IGFBP1, COMP, APCS and LTA4H;PPY, ZCCHC8, CCL25, NFASC and IL4R; IL4R, PPY, CCL25, NFASC and SERPING1; GIP, ADGRE5, HS3ST3B1, APCS and ITPA; IL4R, PPY, INPP1, LTA4H and SERPING1; IL4R, PPY, SULT2A1, COMP and MRPS16; GIP, F12, HS3ST3B1, ADGRE1 and IL4R; PPY, ZCCHC8, SPINT3, CCL25 and NOTCH3; GIP, ADGRE5, HS3ST3B1, APCS and SERPING1; GIP, HS 3ST3B1, ADGRE1, APCS, and SWAP70; IL4R, PPY, INPP1, SPINT3, and STX7; IL4R, PPY, INPP1, LTA4H, and YJU2; PPY, SPINT3, CCL25, NFASC, and SOST; IL4R, PPY, SPINT3, COMP, and ZCCHC8; PPY, CCL25, NFASC, APCS, and SERPING1; IL4R, PPY, INPP1, CCL25, and SERPING1; GIP, ZCCHC8, SPINT3, NTF3, and STX7; IL4R, PPY, SULT2A1, F12, and SWA P70; PPY, INPP1, ZCCHC8, LTA4H, and IL4R; IL4R, PPY, ZCCHC8, SPINT3, and NOTCH3; PPY, ZCCHC8, SPINT3, CCL25, and NTF3; IL4R, PPY, SULT2A1, NTF3, and CLIC5; GIP, ZCCHC8, CCL25, KLK3, and NFASC; PPY, CCL25, NFASC, APCS, and NOTCH3; IL4R, PPY, SWAP70, STX7, and AFM; PPY, ZCCHC8, CCL25, NFASC, and SERPING1; GIP, F12, HS3ST3B 1. NTF3 and KLK3; IL4R, PPY, CCL25, NFASC and ENPP2; IL4R, PPY, SULT2A1, INPP1 and MRPS16; PPY, F12, HS3ST3B1, NTF3 and ZCCHC8; IL4R, TMPRSS15, SULT2A1, COMP and ADGRE1; IL4R, PPY, INPP1, STX7 and SERPING1; PPY, ZCCHC8, SPINT3, CLIC5 and MRPS16; IL4R, IGFBP1, ADGRE5, APCS and PPY; IL4R, PPY, SULT2A1, COMP and STX7;IL4R, PPY, SULT2A1, F12 and ENPP2; IL4R, PPY, CCL25, LPL and STX7; IL4R, PPY, SULT2A1, COMP and NFASC; PPY, CCL25, NFASC, LPL and SERPING1; PPY, F12, HS3ST3B1, NTF3 and SWAP70; GIP, F12, HS3ST3B1, ADGRE1 and FASLG; IL4R, TMPRSS15, SULT2A1, COMP and PPY; IL4R, PPY, SULT2A1, NTF3 and GIP; GIP, ZCCHC8, SPINT3, NTF3 and HS3ST3B1; IL4R, PPY, CCL25, LPL and NFASC; IL4R, PPY, SWAP70, F12 and SPINT3; IL4R, PPY, INPP1, STX7 and IGFBP1; IL4R, PPY, SULT2A1, NFASC and APCS; IL4R, PPY, CCL25, NFASC and LSM1; IL4R, PPY, SULT2A1, NFASC and STX7; IL4R, SPINT3, CLIC5, COMP and LPL; PPY, CCL25, NFASC, LPL and IL4R; IL4R, PPY, F12, ADG RE5 and SERPING1; GIP, F12, HS3ST3B1, ADGRE1 and BLNK; IL4R, PPY, SPINT3, COMP and NTF3; IL4R, PPY, CCL25, NFASC and STX7; PPY, SULT2A1, F12, NTF3 and ZCCHC8; IL4R, PPY, SULT2A1, NFASC and LTA4H; PPY, SULT2A1, CCL25, NFASC and APCS; PPY, SPINT3, CCL25, NFASC and LTA4H; PPY, CCL25, KLK3, NFASC and IGFBP1; PPY, CC L25, NFASC, KAZN and KLK3; PPY, ZCCHC8, SPINT3, CLIC5 and INPP1; PPY, SPINT3, CCL25, NFASC and CASP7; GIP, CCL25, ADGRE1, APCS and HS3ST3B1; IL4R, PPY, SPINT3, COMP and ENPP2; PPY, ZCCHC8, SPINT3, CLIC5 and ITPA; IL4R, PPY, F12, GAMT and NTF3; IL4R, PPY, SPINT3, COMP and YJU2; PPY, SPINT3, CCL25, NFASC and STX7;SWAP70, CLIC5, IGFBP1, STX7 and SPINT3; IL4R, PPY, ADGRE5, APCS and SULT2A1; PPY, F12, HS3ST3B1, NTF3 and SOST; PPY, ZCCHC8, SPINT3, CCL25 and INPP1; IL4R, PPY, SULT2A1, ZCCHC8 and INPP1; IL4R, PPY, F12, GAMT and SULT2A1; IL4R, PPY, ZCCHC8, SPINT3 and PAIP2B; PPY, CCL25, KLK3, NFASC and SPINT3; IL4R, PPY, ADGRE5, APCS and CCL25; IL4R, PPY, ADGRE5, APCS, and GIP; PPY, ZCCHC8, CCL25, NFASC, and IGFBP1; IL4R, PPY, SULT2A1, NTF3, and INPP1; GIP, SULT2A1, F12, NTF3, and TMPRSS15; IL4R, PPY, CCL25, APCS, and ENPP2; SWAP70, CLIC5, IGFBP1, STX7, and APCS; GIP, ZCCHC8, HS3ST3B1, ADGRE1, and STX7; GIP, TMPRSS15, FASLG, ZCCHC8, and F12; or GIP, F12, HS3ST3B1, NTF3, and CCL25.

[0034] Preferably, the five markers are in combination: IL4R, PPY, SULT2A1, F12, and NTF3; IL4R, PPY, SPINT3, COMP, and CLIC5; IL4R, PPY, SPINT3, COMP, and F12; IL4R, PPY, SPINT3, F12, and COMP; PPY, ZCCHC8, SPINT3, CCL25, and NFASC; PPY, ZCCHC8, SPINT3, NTF3, and F12; PPY, F12, HS3ST3B1, NTF3, and SERPING1; IL4R, PPY, SPINT3, COMP, and ITPA; IL4R, PPY, INPP1, LTA4 H and STX7; IL4R, SPINT3, CLIC5, COMP and PPY; GIP, F12, HS3ST3B1, ADGRE1 and NTF3; IL4R, PPY, SULT2A1, COMP and APCS; IL4R, PPY, CCL25, STX7 and ENPP2; GIP, F12, HS3ST3B1, NTF3 and ADGRE1; PPY, SPINT3, CCL25, NFASC and SERPING1; IL4R, PPY, SULT2A1, NTF3 and ZCCHC8; IL4R, PPY, SPINT3, COMP and SERPING1; IL4R, PPY, CC L25, STX7 and NOTCH3; IL4R, PPY, INPP1, LTA4H and ZCCHC8; SULT2A1, IGFBP1, NTF3, ITPA and F12; IL4R, PPY, SULT2A1, NTF3 and F12; PPY, CCL25, KLK3, NFASC and ZCCHC8; GIP, ADGRE5, HS3ST3B1, APCS and LSM1; PPY, CCL25, KLK3, NFASC and STX7; PPY, CCL25, STX7, NFASC and LSM1; PPY, SULT2A1, F12, NTF3 and IL4R; PPY, CCL25, KLK3 K3, NFASC and LSM1; PPY, SPINT3, CCL25, NFASC and ZCCHC8; IL4R, PPY, SPINT3, F12 and SWAP70; IL4R, PPY, SPINT3, COMP and CCL25; IL4R, PPY, SULT2A1, NTF3 and SERPING1; PPY, ZCCHC8, SPINT3, NTF3 and SERPING1; IL4R, PPY, SULT2A1, NFASC and CCL25; IL4R, IGFBP1, COMP, APCS and PPY; GIP, ADGRE5, HS3ST3B1, APCS and CCL25;PPY, SULT2A1, CCL25, NFASC and IL4R; IL4R, PPY, CCL25, NFASC and SULT2A1; IL4R, PPY, SPINT3, COMP and LTA4H; IL4R, PPY, CCL25, NFASC and SPINT3; PPY, SULT2A1, NFASC, NTF3 and IL4R; IL4R, PPY, SPINT3, COMP and STX7; IL4R, PPY, CCL25, STX7 and NFASC; SULT2A1, F12, IGFBP1, NTF3 and PPY; IL4R, PPY, LTA4H, STX7 and SULT2A1; GIP, ZCCHC8, F12, NTF3, and SPINT3; PPY, CCL25, STX7, NFASC, and IL4R; IL4R, PPY, SULT2A1, ZCCHC8, and NTF3; GIP, F12, HS3ST3B1, NTF3, and ZCCHC8; IL4R, PPY, SULT2A1, NTF3, and STX7; IL4R, PPY, SULT2A1, COMP, and F12; GIP, TMPRSS15, FASLG, CCL25, and ZCCHC8; IL4R, PPY, SULT2A1, NFASC, and F12; GIP, CCL25, ADGRE5, APCS, and HS3S T3B1; PPY, ZCCHC8, SPINT3, CLIC5 and COMP; PPY, F12, HS3ST3B1, NTF3 and SULT2A1; IL4R, PPY, SULT2A1, INPP1 and LTA4H; PPY, ZCCHC8, SPINT3, CLIC5 and NTF3; IL 4R, PPY, SPINT3, F12 and NTF3; PPY, INPP1, ZCCHC8, LTA4H and SPINT3; IL4R, PPY, SULT2A1, NTF3 and IGFBP1; PPY, SPINT3, CCL25, NFASC and LSM1; IL4R, PPY, INPP1, LTA4H and SULT2A1; IL4R, PPY, SPINT3, COMP and SOST; PPY, ZCCHC8, CCL25, NFASC and KLK3; GIP, TMPRSS15, CCL25, CASP7 and ENPP2; PPY, ZCCHC8, SPINT3, NFASC and CCL25; PPY, INPP1, LTA4H, KLK3 and ZCCHC8; TMPRSS15, SWAP70, CCL25, STX7 and KLK3; GIP, FASLG, ZCCHC8, CCL25 and ADGRE1; IL4R, PPY, SULT2A1, COMP and SERPING1;GIP, IL4R, SULT2A1, NTF3, and F12; IL4R, PPY, CCL25, STX7, and LPL; SWAP70, IGFBP1, STX7, ITPA, and SPINT3; IL4R, PPY, ADGRE5, APCS, and SERPING1; IL4R, SPINT3, CLIC5, COMP, and ITPA; GIP, HS3ST3B1, ADGRE1, APCS, and NTF3; GIP, ADGRE5, HS3ST3B1, APCS, and IL4R; IL4R, PPY, INPP1, STX7, and LTA4H; GIP, F12, HS3ST3B1, NTF3, and A PCS; PPY, CCL25, NFASC, APCS and CABP2; PPY, INPP1, ZCCHC8, LTA4H and KLK3; PPY, FCRL1, CCL25, NFASC and SERPING1; IL4R, PPY, SULT2A1, COMP and SPINT3; GIP, SULT2A1, F12, NTF3 and NFASC; SWAP70, SPINT3, IGFBP1, STX7 and ITPA; SPINT3, F12, CLIC5, COMP and PPY; PPY, FCRL1, CCL25, NFASC and LSM1; TMPRSS15, SULT2A1, IN PP1, LTA4H and IL4R; IL4R, PPY, SULT2A1, NTF3 and ITPA; IL4R, PPY, LTA4H, STX7 and NOTCH3; PPY, SWAP70, F12, CLIC5 and SPINT3; IL4R, PPY, F12, HS3ST3B1 and SULT2A1; GIP, CCL25, CASP7, NFASC and SPINT3; GIP, FCRL1, ZCCHC8, F12 and FASLG; IL4R, PPY, ZCCHC8, SPINT3 and COMP; SWAP70, CLIC5, IGFBP1, STX7 and KLK3; GIP, FCRL 1. CCL25, NFASC, and CABP2; IL4R, TMPRSS15, SULT2A1, COMP, and APCS; GIP, IL4R, ADGRE5, APCS, and HS3ST3B1; PPY, F12, CCL25, NFASC, and IL4R; GIP, ZCCHC8, CCL25, ADGRE1, and KLK3; TMPRSS15, CCL25, ADGRE5, APCS, and GIP; GIP, IL4R, ZCCHC8, SPINT3, and PPY; GIP, SPINT3, CCL25, CASP7, and ITPA; GIP, PPY, ZCCHC8, SPINT3, and NTF3;GIP, FASLG, FCRL1, ZCCHC8, and CCL25; TMPRSS15, SULT2A1, LTA4H, NFASC, and INPP1; INPP1, SPINT3, CLIC5, COMP, and IL4R; IL4R, PPY, FCRL1, CCL25, and SERPING1; PPY, ZCCHC8, LTA4H, KLK3, and INPP1; GIP, INPP1, ZCCHC8, LTA4H, and PPY; GIP, FASLG, CCL25, NFASC, and TMPRSS15; GIP, SWAP70, FCRL1, F12, and CCL25; PPY, TMPRSS1 5, CCL25, NFASC and SPINT3; TMPRSS15, SWAP70, FASLG, IGFBP1 and STX7; TMPRSS15, CCL25, CASP7, LPL and IL4R; PPY, TMPRSS15, FASLG, CCL25 and ZCCHC8; SWAP70, FCRL1, IGFBP1, STX7 and ITPA; SULT2A1, ZCCHC8, IGFBP1, NTF3 and PPY; GIP, CCL25, STX7, ADGRE5 and KLK3; GIP, FCRL1, F12, ADGRE1 and FASLG; GIP, PPY, FCRL1, ZC CHC8 and SPINT3; SULT2A1, INPP1, IGFBP1, LTA4H and TMPRSS15; TMPRSS15, FASLG, INPP1, LTA4H and SULT2A1; GIP, IL4R, CCL25, NFASC and KLK3; GIP, SULT2A1, FCRL1, ZCCHC8 and F12; GIP, F12, CCL25, NFASC and FCRL1; GIP, SWAP70, FASLG, ZCCHC8 and FCER2; FCRL1, IGFBP1, STX7, ITPA and SWAP70; TMPRSS15, FCRL1, CCL25, STX 7 and NOTCH3; TMPRSS15, SWAP70, SULT2A1, LTA4H and IL4R; GIP, FCRL1, STX7, ITPA and SWAP70; TMPRSS15, INPP1, SPINT3, CLIC5 and COMP; INPP1, CLIC5, SOD3, ITPA and SPINT3; INPP1, CLIC5, KLK3, COMP and IL4R; TMPRSS15, SWAP70, F12, CLIC5 and SPINT3; SULT2A1, IGFBP1, LTA4H, NTF3 and PPY; INPP1, F12, CLIC5, SOD3 and PPY;TMPRSS15, INPP1, CLIC5, SOD3, and LTA4H; GIP, IL4R, FCRL1, ZCCHC8, and FASLG; GIP, IL4R, F12, KAZN, and ADGRE1; INPP1, CLIC5, COMP, SOD3, and APCS; or INPP1, CLIC5, LPL, HS3ST3B1, and ITPA; i.e., any one of the 143 5-marker combinations mentioned above.

[0035] Preferably, the A includes a combination of 6 markers, and the combination of 6 markers is any six of the combinations in B.

[0036] Preferably, the 6 markers are combined into b1) and b2):

[0037] b1) the 5-marker combination (preferably any one of the 143 5-marker combinations mentioned above);

[0038] b2) any one of the above B;

[0039] The b2) is different from any one marker in the combination of 5 markers in b1).

[0040] Preferably, the six markers are: IL4R, PPY, SULT2A1, COMP, SERPING1 and APCS; PPY, F12, HS3ST3B1, NTF3, SERPING1 and SWAP70; IL4R, PPY, SPINT3, COMP, STX7 and LTA4H; IL4R, IGFBP1, COMP, APCS, PPY and FCER2; PPY, F12, HS3ST3B1, NTF3, SERPING1 and LTA4H; SULT2A1, F12, IGFBP1, NTF3, PPY and YJU2; IL4R, PPY, SULT2A1, COMP, F12 and APCS; IL4R, PPY, LTA4H, STX7, SULT2A1 and CCL25; PPY, CCL25, STX7, NFASC, LSM1 and SERPING1; SPINT3, F12, CLIC5, COMP, PPY and IL4R; GIP, F12, HS3ST3B1, ADGRE1, NTF3 and SWAP70; IL4R, PPY, CCL25, STX7, ENPP2 and CLIC5; IL4R, SPINT3, CLIC5, COMP, PPY and APCS; GIP, F12, HS3ST3B1, ADGRE1, NTF3 and YJU2; PPY, F12, HS3ST3B1, NTF3, SULT2A1 and YJU2; IL4R, PPY, SULT2A1, NTF3, F12 and APCS; PPY, ZCCHC8, SPINT3, CLIC5, COMP and APCS; IL4R, PPY, SPINT3, COMP, CCL25 and APCS; IL4R, PPY, SPINT3, COMP, CLIC5 and APCS; IL4R, PPY, SPINT3, F12, COMP and APCS; PPY, CCL25, STX7, NFASC, LSM1 and ENPP2; PPY, CCL25, KLK3, NFASC, S TX7 and ADGRE5; IL4R, PPY, CCL25, STX7, ENPP2 and LTA4H; IL4R, PPY, SULT2A1, COMP, SPINT3 and APCS; SULT2A1, F12, IGFBP1, NTF3, PPY and LTA4H; PPY, F12, HS3ST3B1, NTF3, SULT2A1 and CABP2; IL4R, PPY, CCL25, STX7, NFASC and LTA4H; SULT2A1, F12, IGFBP1, NTF3, PPY and ENPP2; IL4R, PPY, SPINT3, COMP, ITPA and APCS;IL4R, SPINT3, CLIC5, COMP, PPY, and SULT2A1; GIP, F12, HS3ST3B1, ADGRE1, NTF3, and IL4R; PPY, CCL25, STX7, NFASC, LSM1, and FCER2; IL4R, PPY, SPINT3, COMP, SERPING1, and APCS; IL4R, SPINT3, CLIC5, COMP, PPY, and FCRL1; IL4R, PPY, SPINT3, COMP, SERPING1, and SULT2A1; PPY, F12, HS3ST3B1, NTF3, SERPING1, and YJU2; IL4R , PPY, CCL25, STX7, NOTCH3 and LTA4H; IL4R, PPY, SULT2A1, NTF3, STX7 and CABP2; PPY, CCL25, STX7, NFASC, LSM1 and LTA4H; SPINT3, F12, CLIC5, COMP, PPY and SERPING1; PPY, CCL25, STX7, NFASC, IL4R and SERPING1; IL4R, PPY, SULT2A1, COMP, SERPING1 and FCER2; SULT2A1, F12, IGFBP1, NTF3, PPY and HS3ST3B1; SULT2A1, F1 2. IGFBP1, NTF3, PPY, and IL4R; GIP, F12, HS3ST3B1, NTF3, ZCCHC8, and YJU2; GIP, ADGRE5, HS3ST3B1, APCS, CCL25, and FCRL1; IL4R, PPY, SULT2A1, COMP, F12, and FCER2; IL4R, PPY, LTA4H, STX7, SULT2A1, and SERPING1; PPY, SULT2A1, CCL25, NFASC, IL4R, and APCS; PPY, ZCCHC8, SPINT3, NFASC, CCL25, and APCS; PPY, ZCCHC8, SPIN T3, NFASC, CCL25 and APCS; IL4R, PPY, SPINT3, COMP, STX7 and CABP2; IL4R, PPY, SPINT3, COMP, F12 and FCER2; IL4R, PPY, SULT2A1, NTF3, STX7 and SERPING1; IL4R, PPY, SULT2A1, NFASC, CCL25 and APCS; PPY, CCL25, STX7, NFASC, LSM1 and NOTCH3; IL4R, PPY, SULT2A1, COMP, F12 and LTA4H; IL4R, PPY, SPINT3, COMP, F12 and APCS;IL4R, PPY, SPINT3, COMP, CLIC5 and SERPING1; IL4R, IGFBP1, COMP, APCS, PPY and ENPP2; PPY, CCL25, STX7, NFASC, IL4R and HS3ST3B1; IL4R, PPY, SPINT3, COMP, STX7 and APCS; IL4R, PPY, CCL25, STX7, NFASC and HS3ST3B1; GIP, F12, HS3ST3B1, ADGRE1, NTF3 and ENPP2; PPY, F12, HS3ST3B1, NTF3, SERPING1 and NOTCH3; GIP, F12, HS3ST3B1, NTF3, ADGRE1, and LTA4H; IL4R, PPY, SPINT3, COMP, F12, and CABP2; PPY, F12, HS3ST3B1, NTF3, SERPING1, and ADGRE5; PPY, F12, HS3ST3B1, NTF3, SULT2A1, and ENPP2; IL4R, PPY, SPINT3, COMP, F12, and CCL25; IL4R, PPY, CCL25, NFASC, SULT2A1, and APCS; PPY, F12, HS3ST3B1, NTF3, SULT2A1, and SERPING 1; IL4R, PPY, SULT2A1, NTF3, IGFBP1, and APCS; IL4R, PPY, CCL25, STX7, NOTCH3, and ENPP2; FCRL1, IGFBP1, STX7, ITPA, SWAP70, and SERPING1; IL4R, PPY, CCL25, STX7, NFASC, and ENPP2; SPINT3, F12, CLIC5, COMP, PPY, and APCS; PPY, F12, HS3ST3B1, NTF3, SULT2A1, and IGFBP1; PPY, F12, HS3ST3B1, NTF3, SERPING1, and CAS P7; PPY, CCL25, STX7, NFASC, IL4R and LTA4H; IL4R, PPY, INPP1, STX7, LTA4H and SERPING1; IL4R, PPY, CCL25, STX7, NFASC and ADGRE5; SWAP70, IGFBP1, STX7, ITPA, SPINT3 and LTA4H; IL4R, PPY, SULT2A1, NTF3, SERPING1 and BLNK; PPY, CCL25, STX7, NFASC, LSM1 and SWAP70; IL4R, PPY, SPINT3, COMP, CCL25 and LTA4H;SULT2A1, F12, IGFBP1, NTF3, PPY and SWAP70; IL4R, PPY, CCL25, STX7, LPL and LTA4H; PPY, SPINT3, CCL25, NFASC, ZCCHC8 and FCRL1; SPINT3, F12, CLIC5, COMP, PPY and CABP2; PPY, F12, HS3ST3B1, NTF3, SULT2A1 and SWAP70; PPY, FCRL1, CCL25, NFASC, LSM1 and APCS; IL4R, PPY, SULT2A1, COMP, SERPING1 and FCRL1; PPY, F 12, HS3ST3B1, NTF3, SULT2A1, and PRAME; PPY, CCL25, STX7, NFASC, LSM1, and F12; GIP, F12, HS3ST3B1, ADGRE1, NTF3, and APCS; IL4R, PPY, SPINT3, COMP, ITPA, and FCER2; IL4R, PPY, SPINT3, COMP, STX7, and SERPING1; IL4R, SPINT3, CLIC5, COMP, PPY, and LTA4H; PPY, CCL25, STX7, NFASC, LSM1, and SULT2A1; IL4R, SPINT3, CLI C5, COMP, ITPA, and APCS; IL4R, IGFBP1, COMP, APCS, PPY, and LTA4H; IL4R, PPY, CCL25, STX7, ENPP2, and SERPING1; IL4R, IGFBP1, COMP, APCS, PPY, and YJU2; IL4R, PPY, ZCCHC8, SPINT3, COMP, and CCL25; IL4R, PPY, SPINT3, F12, NTF3, and LTA4H; PPY, SPINT3, CCL25, NFASC, LSM1, and APCS; SWAP70, IGFBP1, STX7, ITPA, and SPINT3 and SERPING1; GIP, F12, HS3ST3B1, ADGRE1, NTF3 and SERPING1; PPY, CCL25, STX7, NFASC, LSM1 and ADGRE5; SULT2A1, F12, IGFBP1, NTF3, PPY and ADGRE5; PPY, CCL25, STX7, NFASC, LSM1 and APCS; IL4R, PPY, INPP1, LTA4H, STX7 and CCL25; PPY, CCL25, STX7, NFASC, IL4R and ENPP2; IL4R, PPY, SULT2A1, NTF3, ZCCHC8 and APCS;PPY, CCL25, KLK3, NFASC, STX7 and ENPP2; IL4R, PPY, SPINT3, COMP, STX7 and PRAME; SPINT3, F12, CLIC5, COMP, PPY and FCRL1; PPY, F12, HS3ST3B1, NTF3, SERPING1 and IL4R; TMPRSS15, FCRL1, CCL25, STX7, NOTCH3 and PRAME; PPY, SULT2A1, F12, NTF3, IL4R and APCS; IL4R, PPY, SPINT3, COMP, CCL25 and SULT2A1; IL4R, PPY, CCL25, STX7, ENPP2, and NOTCH3; IL4R, IGFBP1, COMP, APCS, PPY, and SWAP70; IL4R, PPY, CCL25, STX7, NFASC, and YJU2; PPY, CCL25, STX7, NFASC, IL4R, and PRAME; IL4R, PPY, SPINT3, COMP, F12, and SULT2A1; IL4R, PPY, LTA4H, STX7, NOTCH3, and ENPP2; IL4R, PPY, SPINT3, COMP, CLIC5, and FCRL1; PPY, F12, HS3ST3B1, NT F3, SULT2A1 and ADGRE5; PPY, ZCCHC8, SPINT3, NTF3, F12 and APCS; IL4R, PPY, SPINT3, F12, COMP and PRAME; TMPRSS15, FCRL1, CCL25, STX7, NOTCH3 and IGFBP1; GIP, F12, HS3ST3B1, NTF3, APCS and NOTCH3; IL4R, PPY, LTA4H, STX7, NOTCH3 and ADGRE5; IL4R, PPY, SULT2A1, NFASC, CCL25 and FCRL1; IL4R, PPY, LTA4H, STX 7. NOTCH3 and GIP; IL4R, IGFBP1, COMP, APCS, PPY and ADGRE5; IL4R, PPY, SULT2A1, NTF3, F12 and LTA4H; IL4R, PPY, CCL25, STX7, ENPP2 and APCS; IL4R, PPY, CCL25, STX7, NOTCH3 and IGFBP1; SPINT3, F12, CLIC5, COMP, PPY and LTA4H; PPY, CCL25, KLK3, NFASC, STX7 and HS3ST3B1; IL4R, PPY, SPINT3, COMP, CLIC5 and LTA4H;IL4R, SPINT3, CLIC5, COMP, PPY, and FCER2; PPY, FCRL1, CCL25, NFASC, LSM1, and FCER2; PPY, CCL25, NFASC, APCS, CABP2, and FCRL1; IL4R, PPY, SPINT3, F12, COMP, and SERPING1; IL4R, PPY, SPINT3, F12, NTF3, and YJU2; IL4R, PPY, CCL25, STX7, ENPP2, and GIP; PPY, SULT2A1, F12, NTF3, IL4R, and SWAP70; IL4R, PPY, SPINT3, F12, CO MP and FCER2; PPY, ZCCHC8, SPINT3, CLIC5, NTF3 and APCS; IL4R, PPY, SPINT3, COMP, F12 and LTA4H; IL4R, PPY, INPP1, STX7, LTA4H and CCL25; IL4R, PPY, INPP1, LTA4H, STX7 and PRAME; IL4R, PPY, LTA4H, STX7, SULT2A1 and ADGRE5; IL4R, PPY, SULT2A1, NTF3, ZCCHC8 and LTA4H; IL4R, PPY, SULT2A1, COMP, F12 and SERPING1; PPY, ZC CHC8, SPINT3, NTF3, F12, and IL4R; IL4R, PPY, SPINT3, COMP, ITPA, and FCRL1; PPY, SULT2A1, NFASC, NTF3, IL4R, and APCS; PPY, ZCCHC8, SPINT3, NTF3, F12, and ENPP2; GIP, F12, HS3ST3B1, NTF3, ADGRE1, and SWAP70; GIP, F12, HS3ST3B1, NTF3, ADGRE1, and YJU2; IL4R, PPY, SULT2A1, COMP, F12, and PRAME; PPY, SPINT3, CCL25, and NFASC , ZCCHC8 and APCS; GIP, F12, HS3ST3B1, NTF3, ADGRE1 and APCS; IL4R, PPY, CCL25, STX7, NFASC and APCS; GIP, F12, HS3ST3B1, ADGRE1, NTF3 and NOTCH3; PPY, CCL25, STX7, NFASC, LSM1 and YJU2; IL4R, PPY, CCL25, NFASC, SULT2A1 and FCRL1; SULT2A1, IGFBP1, NTF3, ITPA, F12 and SWAP70; IL4R, PPY, INPP1, STX7, LTA4H and IGFBP1;GIP, F12, HS3ST3B1, NTF3, ADGRE1 and ENPP2; IL4R, PPY, SPINT3, COMP, ITPA and ADGRE5; IL4R, PPY, SULT2A1, COMP, APCS and ADGRE5; PPY, F12, HS3ST3B1, NTF3, SERPING1 and CABP2; PPY, SULT2A1, F12, NTF3, IL4R and YJU2; IL4R, PPY, SPINT3, COMP, ITPA and SULT2A1; GIP, F12, HS3ST3B1, ADGRE1, NTF3 and ADGRE5; GIP, F1 2, HS3ST3B1, NTF3, ADGRE1 and FCRL1; IL4R, PPY, SPINT3, F12, NTF3 and APCS; IL4R, PPY, SPINT3, COMP, ITPA and NFASC; PPY, ZCCHC8, SPINT3, NTF3, F12 and SERPING1; GIP, CCL25, ADGRE5, APCS, HS3ST3B1 and FCRL1; IL4R, IGFBP1, COMP, APCS, PPY and PRAME; GIP, F12, HS3ST3B1, ADGRE1, NTF3 and FCRL1; SULT2A1, F12, IGF BP1, NTF3, PPY, and PRAME; IL4R, PPY, SPINT3, COMP, LTA4H, and APCS; PPY, CCL25, STX7, NFASC, IL4R, and SWAP70; IL4R, PPY, LTA4H, STX7, SULT2A1, and GIP; IL4R, PPY, SULT2A1, COMP, APCS, and FCRL1; IL4R, PPY, LTA4H, STX7, SULT2A1, and CABP2; IL4R, PPY, F12, HS3ST3B1, SULT2A1, and IGFBP1; IL4R, PPY, SULT2A1, NTF3, SERP ING1 and APCS; IL4R, PPY, SULT2A1, COMP, SPINT3 and ENPP2; IL4R, PPY, SPINT3, COMP, STX7 and CCL25; GIP, F12, HS3ST3B1, NTF3, APCS and CABP2; IL4R, PPY, SPINT3, COMP, CLIC5 and SULT2A1; PPY, ZCCHC8, SPINT3, NTF3, F12 and ADGRE5; GIP, F12, HS3ST3B1, NTF3, ADGRE1 and PRAME; IL4R, IGFBP1, COMP, APCS, PPY and NOTCH3;PPY, SULT2A1, NFASC, NTF3, IL4R and HS3ST3B1; GIP, F12, HS3ST3B1, NTF3, APCS and SWAP70; IL4R, IGFBP1, COMP, APCS, PPY and SULT2A1; IL4R, PPY, SPINT3, COMP, CLIC5 and ADGRE5; IL4R, PPY, SULT2A1, COMP, SERPING1 and NOTCH3; IL4R, IGFBP1, COMP, APCS, PPY and CABP2; IL4R, PPY, SPINT3, COMP, F12 and NOTCH3; IL4R, P PY, F12, HS3ST3B1, SULT2A1, and CABP2; IL4R, PPY, CCL25, STX7, ENPP2, and HS3ST3B1; IL4R, PPY, SULT2A1, NTF3, ITPA, and APCS; IL4R, PPY, SULT2A1, ZCCHC8, NTF3, and APCS; PPY, ZCCHC8, SPINT3, NTF3, F12, and YJU2; IL4R, PPY, SPINT3, COMP, LTA4H, and ADGRE5; PPY, CCL25, KLK3, NFASC, STX7, and NOTCH3; IL4R, PPY, SPINT3, F1 2. COMP and LTA4H; PPY, ZCCHC8, SPINT3, CLIC5, COMP and IL4R; IL4R, PPY, CCL25, STX7, NFASC and TMPRSS15; GIP, F12, HS3ST3B1, ADGRE1, NTF3 and CABP2; IL4R, PPY, INPP1, LTA4H, STX7 and GIP; PPY, FCRL1, CCL25, NFASC, LSM1 and LTA4H; IL4R, PPY, SULT2A1, NTF3, IGFBP1 and ADGRE5; GIP, ADGRE5, HS3ST3B1, APCS, LSM1 and F CRL1; PPY, ZCCHC8, SPINT3, CCL25, NFASC, and SULT2A1; PPY, CCL25, STX7, NFASC, IL4R, and YJU2; GIP, FCRL1, STX7, ITPA, SWAP70, and IGFBP1; IL4R, PPY, SULT2A1, NTF3, IGFBP1, and SWAP70; PPY, CCL25, STX7, NFASC, IL4R, and CABP2; TMPRSS15, SWAP70, CCL25, STX7, KLK3, and ADGRE5; PPY, CCL25, KLK3, NFASC, ZCCHC8, and APCS;IL4R, PPY, SULT2A1, NTF3, IGFBP1, and HS3ST3B1; IL4R, PPY, SULT2A1, COMP, F12, and FCRL1; PPY, CCL25, KLK3, NFASC, LSM1, and APCS; PPY, CCL25, STX7, NFASC, IL4R, and CLIC5; IL4R, PPY, SPINT3, COMP, CCL25, and ENPP2; IL4R, PPY, LTA4H, STX7, SULT2A1, and IGFBP1; GIP, F12, HS3ST3B1, NTF3, APCS, and SULT2A1; or SPINT3, F12, CLIC5, COMP, PPY, and YJU2.

[0041] Preferably, the six markers are: IL4R, PPY, SULT2A1, COMP, SERPING1 and APCS; PPY, F12, HS3ST3B1, NTF3, SERPING1 and SWAP70; IL4R, PPY, SPINT3, COMP, STX7 and LTA4H; IL4R, IGFBP1, COMP, APCS, PPY and FCER2; PPY, F12, HS3ST3B1, NTF3, SERPING1 and LTA4H; SULT2A1, F12, IGFBP1, NTF3, PPY and YJU2; IL4R, PPY, SULT2A1, COMP, F12 and APCS; IL4R, PPY, LTA4H, STX7, SULT2A1 and CCL25; PPY, CCL25, STX7, NFASC, LSM1 and SERPING1; SPINT3, F12, CLIC5, COMP, PPY and IL4R; GIP, F12, HS3ST3B1, ADGRE1, NTF3 and SWAP70; IL4R, PPY, CCL25, STX7, ENPP2 and CLIC5; IL4R, SPINT3, CLIC5, COMP, PPY and APCS; GIP, F12, HS3ST3B1, ADGRE1, NTF3 and YJU2; PPY, F12, HS3ST3B1, NTF3, SULT2A1 and YJU2; IL4R, PPY, SULT2A1, NTF3, F12 and APCS; PPY, ZCCHC8, SPINT3, CLIC5, COMP and APCS; IL4R, PPY, SPINT3, COMP, CCL25 and APCS; IL4R, PPY, SPINT3, COMP, CLIC5 and APCS; IL4R, PPY, SPINT3, F12, COMP and APCS; PPY, CCL25, STX7, NFASC, LSM1 and ENPP2; PPY, CCL25, KLK3, NFASC, S TX7 and ADGRE5; IL4R, PPY, CCL25, STX7, ENPP2 and LTA4H; IL4R, PPY, SULT2A1, COMP, SPINT3 and APCS; SULT2A1, F12, IGFBP1, NTF3, PPY and LTA4H; PPY, F12, HS3ST3B1, NTF3, SULT2A1 and CABP2; IL4R, PPY, CCL25, STX7, NFASC and LTA4H; SULT2A1, F12, IGFBP1, NTF3, PPY and ENPP2; IL4R, PPY, SPINT3, COMP, ITPA and APCS;IL4R, SPINT3, CLIC5, COMP, PPY, and SULT2A1; GIP, F12, HS3ST3B1, ADGRE1, NTF3, and IL4R; PPY, CCL25, STX7, NFASC, LSM1, and FCER2; IL4R, PPY, SPINT3, COMP, SERPING1, and APCS; IL4R, SPINT3, CLIC5, COMP, PPY, and FCRL1; IL4R, PPY, SPINT3, COMP, SERPING1, and SULT2A1; PPY, F12, HS3ST3B1, NTF3, SERPING1, and YJU2; IL4R , PPY, CCL25, STX7, NOTCH3 and LTA4H; IL4R, PPY, SULT2A1, NTF3, STX7 and CABP2; PPY, CCL25, STX7, NFASC, LSM1 and LTA4H; SPINT3, F12, CLIC5, COMP, PPY and SERPING1; PPY, CCL25, STX7, NFASC, IL4R and SERPING1; IL4R, PPY, SULT2A1, COMP, SERPING1 and FCER2; SULT2A1, F12, IGFBP1, NTF3, PPY and HS3ST3B1; SULT2A1, F1 2. IGFBP1, NTF3, PPY, and IL4R; GIP, F12, HS3ST3B1, NTF3, ZCCHC8, and YJU2; GIP, ADGRE5, HS3ST3B1, APCS, CCL25, and FCRL1; IL4R, PPY, SULT2A1, COMP, F12, and FCER2; IL4R, PPY, LTA4H, STX7, SULT2A1, and SERPING1; PPY, SULT2A1, CCL25, NFASC, IL4R, and APCS; PPY, ZCCHC8, SPINT3, NFASC, CCL25, and APCS; PPY, ZCCHC8, SPIN T3, NFASC, CCL25 and APCS; IL4R, PPY, SPINT3, COMP, STX7 and CABP2; IL4R, PPY, SPINT3, COMP, F12 and FCER2; IL4R, PPY, SULT2A1, NTF3, STX7 and SERPING1; IL4R, PPY, SULT2A1, NFASC, CCL25 and APCS; PPY, CCL25, STX7, NFASC, LSM1 and NOTCH3; IL4R, PPY, SULT2A1, COMP, F12 and LTA4H; IL4R, PPY, SPINT3, COMP, F12 and APCS;IL4R, PPY, SPINT3, COMP, CLIC5 and SERPING1; IL4R, IGFBP1, COMP, APCS, PPY and ENPP2; PPY, CCL25, STX7, NFASC, IL4R and HS3ST3B1; IL4R, PPY, SPINT3, COMP, STX7 and APCS; IL4R, PPY, CCL25, STX7, NFASC and HS3ST3B1; GIP, F12, HS3ST3B1, ADGRE1, NTF3 and ENPP2; PPY, F12, HS3ST3B1, NTF3, SERPING1 and NOTCH3; GIP, F12, HS3ST3B1, NTF3, ADGRE1, and LTA4H; IL4R, PPY, SPINT3, COMP, F12, and CABP2; PPY, F12, HS3ST3B1, NTF3, SERPING1, and ADGRE5; PPY, F12, HS3ST3B1, NTF3, SULT2A1, and ENPP2; IL4R, PPY, SPINT3, COMP, F12, and CCL25; IL4R, PPY, CCL25, NFASC, SULT2A1, and APCS; PPY, F12, HS3ST3B1, NTF3, SULT2A1, and SERPING 1; IL4R, PPY, SULT2A1, NTF3, IGFBP1, and APCS; IL4R, PPY, CCL25, STX7, NOTCH3, and ENPP2; FCRL1, IGFBP1, STX7, ITPA, SWAP70, and SERPING1; IL4R, PPY, CCL25, STX7, NFASC, and ENPP2; SPINT3, F12, CLIC5, COMP, PPY, and APCS; PPY, F12, HS3ST3B1, NTF3, SULT2A1, and IGFBP1; PPY, F12, HS3ST3B1, NTF3, SERPING1, and CAS P7; PPY, CCL25, STX7, NFASC, IL4R and LTA4H; IL4R, PPY, INPP1, STX7, LTA4H and SERPING1; IL4R, PPY, CCL25, STX7, NFASC and ADGRE5; SWAP70, IGFBP1, STX7, ITPA, SPINT3 and LTA4H; IL4R, PPY, SULT2A1, NTF3, SERPING1 and BLNK; PPY, CCL25, STX7, NFASC, LSM1 and SWAP70; IL4R, PPY, SPINT3, COMP, CCL25 and LTA4H;SULT2A1, F12, IGFBP1, NTF3, PPY and SWAP70; IL4R, PPY, CCL25, STX7, LPL and LTA4H; PPY, SPINT3, CCL25, NFASC, ZCCHC8 and FCRL1; SPINT3, F12, CLIC5, COMP, PPY and CABP2; PPY, F12, HS3ST3B1, NTF3, SULT2A1 and SWAP70; PPY, FCRL1, CCL25, NFASC, LSM1 and APCS; IL4R, PPY, SULT2A1, COMP, SERPING1 and FCRL1; PPY, F 12, HS3ST3B1, NTF3, SULT2A1, and PRAME; PPY, CCL25, STX7, NFASC, LSM1, and F12; GIP, F12, HS3ST3B1, ADGRE1, NTF3, and APCS; IL4R, PPY, SPINT3, COMP, ITPA, and FCER2; IL4R, PPY, SPINT3, COMP, STX7, and SERPING1; IL4R, SPINT3, CLIC5, COMP, PPY, and LTA4H; PPY, CCL25, STX7, NFASC, LSM1, and SULT2A1; IL4R, SPINT3, CLI C5, COMP, ITPA, and APCS; IL4R, IGFBP1, COMP, APCS, PPY, and LTA4H; IL4R, PPY, CCL25, STX7, ENPP2, and SERPING1; IL4R, IGFBP1, COMP, APCS, PPY, and YJU2; IL4R, PPY, ZCCHC8, SPINT3, COMP, and CCL25; IL4R, PPY, SPINT3, F12, NTF3, and LTA4H; PPY, SPINT3, CCL25, NFASC, LSM1, and APCS; SWAP70, IGFBP1, STX7, ITPA, and SPINT3 and SERPING1; GIP, F12, HS3ST3B1, ADGRE1, NTF3 and SERPING1; PPY, CCL25, STX7, NFASC, LSM1 and ADGRE5; SULT2A1, F12, IGFBP1, NTF3, PPY and ADGRE5; PPY, CCL25, STX7, NFASC, LSM1 and APCS; IL4R, PPY, INPP1, LTA4H, STX7 and CCL25; PPY, CCL25, STX7, NFASC, IL4R and ENPP2; IL4R, PPY, SULT2A1, NTF3, ZCCHC8 and APCS;PPY, CCL25, KLK3, NFASC, STX7 and ENPP2; IL4R, PPY, SPINT3, COMP, STX7 and PRAME; SPINT3, F12, CLIC5, COMP, PPY and FCRL1; PPY, F12, HS3ST3B1, NTF3, SERPING1 and IL4R; TMPRSS15, FCRL1, CCL25, STX7, NOTCH3 and PRAME; PPY, SULT2A1, F12, NTF3, IL4R and APCS; IL4R, PPY, SPINT3, COMP, CCL25 and SULT2A1; IL4R, PPY, CCL25, STX7, ENPP2, and NOTCH3; IL4R, IGFBP1, COMP, APCS, PPY, and SWAP70; IL4R, PPY, CCL25, STX7, NFASC, and YJU2; PPY, CCL25, STX7, NFASC, IL4R, and PRAME; IL4R, PPY, SPINT3, COMP, F12, and SULT2A1; IL4R, PPY, LTA4H, STX7, NOTCH3, and ENPP2; IL4R, PPY, SPINT3, COMP, CLIC5, and FCRL1; PPY, F12, HS3ST3B1, NT F3, SULT2A1 and ADGRE5; PPY, ZCCHC8, SPINT3, NTF3, F12 and APCS; IL4R, PPY, SPINT3, F12, COMP and PRAME; TMPRSS15, FCRL1, CCL25, STX7, NOTCH3 and IGFBP1; GIP, F12, HS3ST3B1, NTF3, APCS and NOTCH3; IL4R, PPY, LTA4H, STX7, NOTCH3 and ADGRE5; IL4R, PPY, SULT2A1, NFASC, CCL25 and FCRL1; IL4R, PPY, LTA4H, STX 7. NOTCH3 and GIP; IL4R, IGFBP1, COMP, APCS, PPY and ADGRE5; IL4R, PPY, SULT2A1, NTF3, F12 and LTA4H; IL4R, PPY, CCL25, STX7, ENPP2 and APCS; IL4R, PPY, CCL25, STX7, NOTCH3 and IGFBP1; SPINT3, F12, CLIC5, COMP, PPY and LTA4H; PPY, CCL25, KLK3, NFASC, STX7 and HS3ST3B1; IL4R, PPY, SPINT3, COMP, CLIC5 and LTA4H;IL4R, SPINT3, CLIC5, COMP, PPY, and FCER2; PPY, FCRL1, CCL25, NFASC, LSM1, and FCER2; PPY, CCL25, NFASC, APCS, CABP2, and FCRL1; IL4R, PPY, SPINT3, F12, COMP, and SERPING1; IL4R, PPY, SPINT3, F12, NTF3, and YJU2; IL4R, PPY, CCL25, STX7, ENPP2, and GIP; PPY, SULT2A1, F12, NTF3, IL4R, and SWAP70; IL4R, PPY, SPINT3, F12, COMP, and FCER2; PPY, ZCCHC8, SPINT3, CLIC5, NTF3, and APCS; IL4R, PPY, SPINT3, COMP, F12 and LTA4H; IL4R, PPY, INPP1, STX7, LTA4H, and CCL25; IL4R, PPY, INPP1, LTA4H, STX7, and PRAME; IL4R, PPY, LTA4H, STX7, SULT2A1, and ADGRE5; IL4R, PPY, SULT2A1, NTF3, ZCCHC8, and LTA4H; IL4R, PPY, SULT2A1, COMP, F12, and SERPING1; PPY, ZCCHC8, SPINT3, NTF3, F12, and IL4R; IL4R, PPY, SPINT3, COMP, ITPA, and FCRL1; PPY, SULT2A1, NFASC, NTF3, IL4R, and APCS; or PPY, ZCCHC8, SPINT3, NTF3, F12, and ENPP2; i.e., any one of the above 163 combinations of 6 markers.

[0042] Preferably, the A comprises a combination of 7 markers, and the combination of 7 markers is any seven of the combinations in B.

[0043] Preferably, the 7 markers are combined into c1) and c2):

[0044] c1) the 6 markers combination (preferably any one of the 163 6 markers combinations mentioned above);

[0045] c2) any one of the above B;

[0046] The c2) is different from any one marker in the combination of 6 markers in c1).

[0047] Preferably, the 7 marker combination is: IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1 and ADGRE5; PPY, SULT2A1, F12, NTF3, IL4R, SWAP70 and LTA4H; IL4R, PPY, LTA4H, STX7, SULT2A1, SERPING1 and HS3ST3B1; IL4R, PPY, SULT2A1, COMP, SERPING1, APCS and SOST; PPY, CCL25, STX7, NFASC, LSM1, SERPING1 and APCS; IL4R, PPY, SPINT3, COMP, S TX7, SERPING1, and ENPP2; IL4R, PPY, SPINT3, COMP, F12, LTA4H, and HS3ST3B1; PPY, CCL25, STX7, NFASC, LSM1, ENPP2, and NOTCH3; IL4R, PPY, SULT2A1, COMP, F12, SERPING1, and KAZN; IL4R, PPY, INPP1, STX7, LTA4H, SERPING1, and KAZN; IL4R, PPY, SPINT3, COMP, CLIC5, APCS, and LTA4H; PPY, F12, SULT2A1, IGFBP1, and NTF 3. IL4R and ENPP2; IL4R, PPY, SPINT3, F12, COMP, PRAME and TEX101; IL4R, PPY, SPINT3, COMP, CLIC5, SERPING1 and TEX101; IL4R, PPY, SULT2A1, NTF3, F12, APCS and TEX101; IL4R, PPY, CCL25, STX7, NFASC, YJU2 and ITPA; IL4R, PPY, SPINT3, COMP, STX7, LTA4H and CCL25; IL4R, PPY, CCL25, STX7, ENPP2, CLIC5 and KAZN ;IL4R, PPY, SPINT3, COMP, SERPING1, APCS and ITPA;IL4R, PPY, LTA4H, STX7, NOTCH3, ENPP2 and CCL25;IL4R, PPY, LTA4H, STX7, NOTCH3, ENPP2 and CCL25;IL4R, PPY, SPINT3, COMP, F12, SULT2A1 and HS3ST3B1;IL4R, PPY, SPINT3, COMP, F12, SULT2A1 and HS3ST3B1;PPY, CCL25, STX7, NFASC, IL4R, SERPING1 and APCS;GIP, F12, HS3ST3B1, ADGRE1, NTF3, APCS, and KAZN; PPY, F12, SPINT3, CLIC5, COMP, IL4R, and NTF3; IL4R, PPY, SULT2A1, NTF3, F12, LTA4H, and HS3ST3B1; IL4R, PPY, SPINT3, COMP, F12, CCL25, and TEX101; GIP, F12, HS3ST3B1, ADGRE1, NTF3, YJU2, and PRAME; IL4R, PPY, COMP, APCS, IGFBP1, ENPP2, and ITPA; IL4R, PPY, CCL25, ST X7, ENPP2, SERPING1 and NOTCH3; IL4R, PPY, SPINT3, F12, COMP, SERPING1 and PRAME; IL4R, PPY, INPP1, LTA4H, STX7, PRAME and KAZN; PPY, CCL25, STX7, NFASC, LSM1, FCER2 and APCS; PPY, CCL25, STX7, NFASC, LSM1, F12 and APCS; IL4R, PPY, SPINT3, F12, COMP, APCS and ADGRE5; IL4R, SPINT3, CLIC5, COMP, PPY, FCER2 and KAZN; IL4R, SPINT3, CLIC5, COMP, PPY, APCS and ADGRE5; IL4R, PPY, CCL25, STX7, NFASC, HS3ST3B1 and KAZN; IL4R, PPY, COMP, APCS, IGFBP1, FCER2 and LTA4H; IL4R, PPY, SPINT3, COMP, F12, CABP2 and KAZN; PPY, SULT2A1, F12, NTF3, IL4R, APCS and KAZN; PPY, F12, SPINT3, CLIC5, COMP, APCS and NTF3; IL4R, SPINT3, CLIC5, COMP, PPY, LTA4H and HS3ST3B1; IL4R, PPY, SPINT3, COMP, SERPING1, SULT2A1 and APCS; IL4R, PPY, SPINT3, COMP, STX7, APCS and CLIC5; IL4R, PPY, SPINT3, F12, NTF3, LTA4H and CCL25; PPY, ZCCHC8, SPINT3, NTF3, F12, IL4R and LTA4H; IL4R, PPY, SPINT3, COMP, ITPA, FCER2 and LTA4H; IL4R, PPY, INPP1, LTA4H, STX7, CCL25 and KAZN;IL4R, PPY, CCL25, STX7, ENPP2, APCS and ITPA; IL4R, PPY, CCL25, STX7, NOTCH3, IGFBP1 and ENPP2; IL4R, PPY, CCL25, STX7, ENPP2, NOTCH3 and SOST; IL4R, PPY, SPINT3, F12, COMP, FCER2 and NTF3; IL4R, SPINT3, CLIC5, COMP, ITPA, APCS and ADGRE5; PPY, F12, HS3ST3B1, NTF3, SERPING1, LTA4H and CCL25; PPY, CCL25, STX7, NFASC, LSM1, APCS, and SOST; IL4R, PPY, LTA4H, STX7, NOTCH3, GIP, and TEX101; IL4R, PPY, CCL25, STX7, NOTCH3, ENPP2, and HS3ST3B1; IL4R, PPY, LTA4H, STX7, SULT2A1, CCL25, and PRAME; IL4R, PPY, SULT2A1, NTF3, IGFBP1, APCS, and KAZN; PPY, CCL25, STX7, NFASC, LSM1, NOTCH3, and APCS; GIP, F12, HS3ST3B1, ADG RE1, NTF3, SWAP70, and ITPA; GIP, F12, HS3ST3B1, ADGRE1, NTF3, IL4R, and ENPP2; PPY, ZCCHC8, SPINT3, NTF3, F12, ENPP2, and SOST; PPY, F12, HS3ST3B1, NTF3, SERPING1, IL4R, and CCL25; IL4R, PPY, COMP, APCS, IGFBP1, LTA4H, and SOST; IL4R, PPY, COMP, APCS, IGFBP1, ADGRE5, and CCL25; IL4R, SPINT3, CLIC5, COMP, PPY , SULT2A1 and ITPA; IL4R, PPY, SPINT3, COMP, STX7, PRAME and ENPP2; PPY, F12, HS3ST3B1, NTF3, SULT2A1, SERPING1 and SOST; PPY, CCL25, STX7, NFASC, LSM1, ADGRE5 and APCS; PPY, CCL25, STX7, NFASC, LSM1, LTA4H and ITPA; IL4R, PPY, SPINT3, COMP, F12, FCER2 and LTA4H; IL4R, PPY, CCL25, STX7, LPL, LTA4H and ITPA;PPY, CCL25, STX7, NFASC, IL4R, ENPP2 and KAZN; IL4R, PPY, ZCCHC8, SPINT3, COMP, CCL25 and ITPA; IL4R, PPY, SPINT3, COMP, F12, APCS and SULT2A1; IL4R, PPY, CCL25, STX7, ENPP2, LTA4H and KAZN; PPY, F12, HS3ST3B1, NTF3, SERPING1, NOTCH3 and ITPA; IL4R, PPY, LTA4H, STX7, SULT2A1, ADGRE5 and KAZN; PPY, F12, HS3ST3B1, NTF3, SULT2A1, CABP2 and ENPP2; GIP, F12, HS3ST3B1, NTF3, ADGRE1, LTA4H and KAZN; IL4R, PPY, SPINT3, COMP, CLIC5, FCRL1 and HS3ST3B1; GIP, F 12. HS3ST3B1, ADGRE1, NTF3, SERPING1 and LTA4H; IL4R, PPY, SPINT3, COMP, CCL25, LTA4H and ITPA; IL4R, PPY, LTA4H, STX7, NOTCH3, ADGRE5 and PRAME; IL4R , PPY, SULT2A1, COMP, SERPING1, FCRL1 and ENPP2; IL4R, PPY, COMP, APCS, IGFBP1, SWAP70 and CLIC5; IL4R, PPY, SPINT3, COMP, CCL25, SULT2A1 and LTA4H; IL4R, PPY, CCL25, STX7, NFASC, ENPP2 and APCS; PPY, F12, SULT2A1, IGFBP1, NTF3, SWAP70 and ADGRE5; IL4R, PPY, SULT2A1, COMP, SERPING1, FCER2 and ENPP2; I L4R, PPY, CCL25, STX7, NOTCH3, LTA4H and HS3ST3B1; PPY, F12, HS3ST3B1, NTF3, SULT2A1, IGFBP1 and APCS; IL4R, PPY, SULT2A1, COMP, SPINT3, APCS and LTA4 H; GIP, F12, HS3ST3B1, NTF3, APCS, NOTCH3 and ITPA; GIP, F12, HS3ST3B1, NTF3, ZCCHC8, YJU2 and ITPA; PPY, F12, HS3ST3B1, NTF3, SERPING1, YJU2 and CCL25;IL4R, PPY, CCL25, STX7, NFASC, LTA4H and KAZN; IL4R, PPY, CCL25, NFASC, SULT2A1, APCS and LTA4H; PPY, CCL25, STX7, NFASC, IL4R, HS3ST3B1 and APCS; GIP, AD GRE5, HS3ST3B1, APCS, CCL25, FCRL1 and PRAME; GIP, F12, HS3ST3B1, ADGRE1, NTF3, ENPP2 and SULT2A1; IL4R, PPY, SULT2A1, NTF3, ZCCHC8, APCS and LTA4H; PPY, F12, SPINT3, CLIC5, COMP, CABP2, and NTF3; PPY, SULT2A1, CCL25, NFASC, IL4R, APCS, and HS3ST3B1; IL4R, PPY, CCL25, STX7, ENPP2, GIP, and TEX101; IL4R, PPY, SULT2A1, COMP, F12, APCS, and ADGRE5; IL4R, PPY, SULT2A1, NFASC, CCL25, APCS, and ADGRE5; IL4R, PPY, SULT2A1, NTF3, STX7, CABP2, and HS3ST3B1; PPY, F12, SULT2A 1, IGFBP1, NTF3, HS3ST3B1, and ENPP2; PPY, F12, SULT2A1, IGFBP1, NTF3, YJU2, and CCL25; IL4R, PPY, SULT2A1, NTF3, SERPING1, BLNK, and ADGRE5; PPY, F12, SPINT3, CLIC5, COMP, YJU2, and KAZN; IL4R, PPY, SPINT3, COMP, ITPA, FCRL1, and ENPP2; PPY, CCL25, STX7, NFASC, IL4R, PRAME, and APCS; IL4R, PPY, SPINT3, COMP, ITPA , APCS and LTA4H; IL4R, PPY, SPINT3, COMP, CLIC5, LTA4H and PRAME; IL4R, PPY, SPINT3, F12, NTF3, YJU2 and HS3ST3B1; IL4R, PPY, COMP, APCS, IGFBP1, YJU2 and KAZN; IL4R, PPY, SULT2A1, COMP, F12, LTA4H and NTF3; PPY, F12, SULT2A1, IGFBP1, NTF3, NOTCH3 and ITPA; SWAP70, IGFBP1, STX7, ITPA, SPINT3, SERPING1 and SOST;PPY, SULT2A1, NFASC, NTF3, IL4R, APCS and HS3ST3B1; PPY, CCL25, STX7, NFASC, IL4R, LTA4H and PRAME; PPY, F12, SPINT3, CLIC5, COMP, SERPING1 and TEX101; IL4R, PPY, SPINT3, COMP, CCL25, APCS and ADGRE5; IL4R, SPINT3, CLIC5, COMP, PPY, FCRL1 and KAZN; PPY, F12, SPINT3, CLIC5, COMP, LTA4H and TEX101; PPY, F12, SULT2A1, IGFBP1, NTF3, ADGRE5, and PRAME; PPY, CCL25, STX7, NFASC, LSM1, SULT2A1, and ENPP2; PPY, F12, SULT2A1, IGFBP1, NTF3, LTA4H, and HS3ST3B1; PPY, F12, HS3ST3B1, NTF3, SERPING1, ADGRE5, and SOST; IL4R, PPY, INPP1, STX7, LTA4H, CCL25, and APCS; IL4R, PPY, SPINT3, COMP, STX7, CABP2, and HS3ST3B1; I L4R, PPY, CCL25, STX7, NFASC, ADGRE5, and HS3ST3B1; PPY, SULT2A1, F12, NTF3, IL4R, SWAP70, and SOST; PPY, CCL25, STX7, NFASC, IL4R, SERPING1, and NOTCH3; IL4R, PPY, SPINT3, COMP, STX7, SERPING1, and LTA4H; PPY, CCL25, STX7, NFASC, LSM1, SERPING1, and LTA4H; PPY, F12, SULT2A1, IGFBP1, NTF3, IL4R, and LTA4H; IL4R, PPY, SPINT3, COMP, STX7, SERPING1, and PRAME; IL4R, PPY, SPINT3, COMP, CLIC5, APCS, and ADGRE5; PPY, CCL25, STX7, NFASC, IL4R, SERPING1, and SOST; IL4R, PPY, CCL25, STX7, ENPP2, SERPING1, and NTF3; IL4R, PPY, SPINT3, COMP, CLIC5, SERPING1, and LTA4H; IL4R, PPY, SPINT3, COMP, STX7, SERPING1, and APCS;IL4R, PPY, SPINT3, COMP, STX7, SERPING1, and HS3ST3B1; IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, and KAZN; IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, and SOST; PPY, F12, SULT2A1, IGFBP1, NTF3, IL4R, and APCS; IL4R, PPY, CCL25, STX7, NOTCH3, IGFBP1, and NTF3; IL4R, PPY, SPINT3, COMP, F12, CCL25, and ITPA; PPY, CCL2 5, STX7, NFASC, LSM1, SERPING1, and ENPP2; IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, and HS3ST3B1; IL4R, PPY, SPINT3, COMP, STX7, SERPING1, and ADGRE5; IL4R, PPY, SPINT3, COMP, STX7, LTA4H, and HS3ST3B1; IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, and TEX101; IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, and CCL25; P PY, CCL25, STX7, NFASC, LSM1, SERPING1, and ADGRE5; IL4R, PPY, SPINT3, COMP, SERPING1, APCS, and SOST; PPY, F12, SULT2A1, IGFBP1, NTF3, IL4R, and KAZN; IL4R, PPY, SULT2A1, COMP, SERPING1, APCS, and ADGRE5; IL4R, PPY, SPINT3, COMP, STX7, SERPING1, and TEX101; IL4R, PPY, LTA4H, STX7, SULT2A1, SERPING1, and TEX101 ;IL4R, PPY, INPP1, STX7, LTA4H, SERPING1, and ENPP2;IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, and NOTCH3;PPY, CCL25, STX7, NFASC, IL4R, SERPING1, and ADGRE5;IL4R, PPY, SPINT3, F12, COMP, PRAME, and ENPP2;IL4R, PPY, SPINT3, COMP, CLIC5, SERPING1, and SOST;IL4R, PPY, CCL25, STX7, ENPP2, SERPING1, and ADGRE5;PPY, F12, SULT2A1, IGFBP1, NTF3, IL4R and HS3ST3B1; IL4R, PPY, SPINT3, COMP, STX7, SERPING1 and KAZN; IL4R, PPY, SULT2A1, NTF3, F12, APCS and KAZN; PPY, CCL25, STX7, NFASC, LSM1, SERPING1 and NOTCH3; IL4R, PPY, SULT2A1, NTF3, F12, APCS and SOST; IL4R, PPY, SPINT3, COMP, F12, LTA4H and KAZN; GIP, F12, HS3ST3B1, A DGRE1, NTF3, IL4R and ITPA; IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1 and ENPP2; IL4R, PPY, SPINT3, COMP, F12, CCL25 and HS3ST3B1; GIP, F12, HS3ST3B1, ADGRE1, NTF3, APCS and ITPA; PPY, F12, SULT2A1, IGFBP1, NTF3, IL4R and TEX101; IL4R, PPY, SPINT3, COMP, CLIC5, SERPING1 and ITPA; IL4R, PPY, SPINT3, COMP, STX7, S ERPING1 and CCL25; GIP, F12, HS3ST3B1, ADGRE1, NTF3, SERPING1 and APCS; IL4R, PPY, SPINT3, COMP, SERPING1, SULT2A1 and LTA4H; IL4R, PPY, SPINT3, F12, COMP, SERPING1 and CCL25; IL4R, PPY, SULT2A1, COMP, F12, APCS and KAZN; IL4R, PPY, CCL25, STX7, NOTCH3, IGFBP1 and HS3ST3B1; IL4R, PPY, SPINT3, COMP, CLIC5, APC S and TEX101; IL4R, PPY, CCL25, STX7, ENPP2, SERPING1 and ITPA; PPY, F12, SPINT3, CLIC5, COMP, IL4R and HS3ST3B1; IL4R, PPY, SULT2A1, NTF3, IGFBP1, APCS and CCL25; PPY, CCL25, STX7, NFASC, LSM1, SERPING1 and PRAME; IL4R, PPY, INPP1, STX7, LTA4H, SERPING1 and ADGRE5; IL4R, PPY, SPINT3, COMP, F12, SULT2A1 and PRAME;IL4R, SPINT3, CLIC5, COMP, PPY, FCER2, and ITPA; IL4R, SPINT3, CLIC5, COMP, PPY, APCS, and CCL25; IL4R, PPY, SULT2A1, COMP, SERPING1, APCS, and LTA4H; PPY, F12, SULT2A1, IGFBP1, NTF3, IL4R, and ITPA; IL4R, PPY, SPINT3, COMP, STX7, PRAME, and NTF3; IL4R, PPY, SPINT3, COMP, F12, APCS, and KAZN; IL4R, SPINT3, CLIC 5, COMP, PPY, LTA4H and CCL25; IL4R, PPY, COMP, APCS, IGFBP1, YJU2 and ITPA; IL4R, PPY, SPINT3, COMP, F12, CCL25 and APCS; PPY, F12, SPINT3, CLIC5, COMP, IL4R and ITPA; PPY, SULT2A1, F12, NTF3, IL4R, SWAP70 and ADGRE5; PPY, CCL25, STX7, NFASC, LSM1, SERPING1 and KAZN; IL4R, PPY, SULT2A1, NTF3, IGFBP1, APCS and HS3ST3B1; IL4R, PPY, SULT2A1, COMP, F12, SERPING1 and ITPA; IL4R, PPY, LTA4H, STX7, SULT2A1, SERPING1 and KAZN; IL4R, PPY, SULT2A1, COMP, F12, SERPI NG1 and HS3ST3B1; IL4R, PPY, INPP1, STX7, LTA4H, SERPING1 and ITPA; IL4R, PPY, SPINT3, COMP, CLIC5, SERPING1 and APCS; IL4R, PPY, COMP, APCS, IGFBP1, AD GRE5 and HS3ST3B1; IL4R, PPY, SPINT3, COMP, CLIC5, APCS and ENPP2; PPY, F12, SULT2A1, IGFBP1, NTF3, IL4R and SOST; PPY, F12, SPINT3, CLIC5, COMP, APCS and HS3ST3B1; IL4R, PPY, SPINT3, COMP, STX7, SERPING1 and ITPA; IL4R, PPY, SPINT3, F12, COMP, PRAME and KAZN; GIP, F12, HS3ST3B1, NTF3, ZCCHC8, YJU2 and KAZN;IL4R, PPY, SPINT3, COMP, STX7, SERPING1, and CASP7; IL4R, PPY, CCL25, STX7, ENPP2, NOTCH3, and TEX101; IL4R, PPY, SPINT3, COMP, SERPING1, SULT2A1, and NTF3; IL4R, PPY, ZCCHC8, SPINT3, COMP, CCL25, and ADGRE5; IL4R, SPINT3, CLIC5, COMP, PPY, APCS, and NTF3; IL4R, PPY, CCL25, STX7, NFASC, HS3ST3B1, and ENPP2; IL4R , PPY, SPINT3, COMP, F12, SULT2A1 and KAZN; IL4R, PPY, CCL25, STX7, ENPP2, APCS and TEX101; IL4R, PPY, SPINT3, COMP, F12, CABP2 and HS3ST3B1; IL4R, PPY, INPP1, STX7, LTA4H, SERPING1 and CCL25; IL4R, SPINT3, CLIC5, COMP, PPY, APCS and LTA4H; IL4R, PPY, SPINT3, COMP, F12, APCS and HS3ST3B1; GIP, F12, HS3ST3B1, A DGRE1, NTF3, YJU2 and ITPA; IL4R, PPY, CCL25, STX7, ENPP2, SERPING1 and APCS; IL4R, PPY, SPINT3, COMP, F12, SULT2A1 and CCL25; IL4R, PPY, CCL25, STX7, NOTCH3, ENPP2 and APCS; PPY, F12, HS3ST3B1, NTF3, SULT2A1, CABP2 and SOST; IL4R, PPY, SULT2A1, COMP, SERPING1, APCS and CCL25; IL4R, PPY, CCL25, STX7, ENPP2, A PCS and HS3ST3B1; IL4R, PPY, SPINT3, F12, COMP, SERPING1 and ENPP2; PPY, CCL25, STX7, NFASC, LSM1, ENPP2 and HS3ST3B1; IL4R, PPY, CCL25, STX7, LPL, LTA4H and ENPP2; IL4R, PPY, SPINT3, COMP, CLIC5, SERPING1 and KAZN; PPY, F12, HS3ST3B1, NTF3, SERPING1, IL4R and KAZN; PPY, F12, SPINT3, CLIC5, COMP, IL4R and SOD3;IL4R, PPY, SPINT3, F12, COMP, FCER2 and KAZN; PPY, CCL25, STX7, NFASC, IL4R, SERPING1 and LTA4H; IL4R, PPY, COMP, APCS, IGFBP1, ENPP2 and LTA4H; IL4R, PP Y, SPINT3, F12, COMP, APCS and KAZN; IL4R, PPY, SPINT3, COMP, SERPING1, APCS and ADGRE5; IL4R, PPY, SULT2A1, COMP, SPINT3, APCS and ITPA; IL4R, PPY, SPINT 3, COMP, F12, APCS and NTF3; IL4R, SPINT3, CLIC5, COMP, PPY, FCRL1 and ITPA; IL4R, PPY, SPINT3, COMP, CLIC5, APCS and ITPA; PPY, CCL25, STX7, NFASC, LSM1, FCER2 and NOTCH3; PPY, F12, SPINT3, CLIC5, COMP, APCS and TEX101; PPY, CCL25, STX7, NFASC, LSM1, SERPING1 and SOST; IL4R, PPY, SPINT3, COMP, F12, CCL25 and CL IC5; IL4R, PPY, SULT2A1, NTF3, F12, LTA4H, and TEX101; GIP, F12, HS3ST3B1, ADGRE1, NTF3, SERPING1, and CCL25; IL4R, PPY, LTA4H, STX7, SULT2A1, SERPING1, and NOTCH3; IL4R, PPY, CCL25, STX7, NOTCH3, IGFBP1, and APCS; PPY, CCL25, STX7, NFASC, LSM1, APCS, and ADGRE5; IL4R, PPY, CCL25, STX7, ENPP2, SERPING1, and SO ST; IL4R, PPY, SPINT3, COMP, SERPING1, APCS and CASP7; PPY, F12, HS3ST3B1, NTF3, SULT2A1, IGFBP1 and PRAME; IL4R, PPY, LTA4H, STX7, NOTCH3, ADGRE5 and TEX101; IL4R, PPY, SPINT3, F12, COMP, SERPING1 and ITPA; IL4R, PPY, INPP1, STX7, LTA4H, SERPING1 and PRAME; IL4R, PPY, COMP, APCS, IGFBP1, ADGRE5 and SOD3;IL4R, PPY, CCL25, STX7, ENPP2, SERPING1 and KAZN; IL4R, PPY, CCL25, STX7, NFASC, YJU2 and TEX101; IL4R, PPY, SPINT3, COMP, SERPING1, APCS and HS3ST3B1; IL4R, PPY, SULT2A1, NTF3, IGFBP1, APCS and SOST; IL4R, PPY, SULT2A1, NTF3, IGFBP1, APCS and ADGRE5; PPY, F12, SULT2A1, IGFBP1, NTF3, HS3ST3B1 and ITPA; PPY, F12, SPINT3, CLIC5, COMP, IL4R and CCL25; IL4R, PPY, SPINT3, COMP, F12, FCER2 and HS3ST3B1; IL4R, PPY, SPINT3, COMP, F12, LTA4H and NTF3; IL4R, PPY, SPINT3, COMP, F12, LTA4H and SOD3; IL4R, PPY, SPINT3, COMP, CLIC5, SERPING1 and HS3ST3B1; IL4R, PPY, SPINT3, COMP, SERPING1, APCS and ENPP2; PPY, F12, SULT2A1, I GFBP1, NTF3, SWAP70, and PRAME; IL4R, PPY, SPINT3, COMP, STX7, LTA4H, and ITPA; IL4R, PPY, CCL25, STX7, NFASC, ENPP2, and HS3ST3B1; IL4R, PPY, SPINT3, COMP, F12, APCS, and ITPA; PPY, F12, HS3ST3B1, NTF3, SERPING1, IL4R, and ADGRE5; GIP, F12, HS3ST3B1, ADGRE1, NTF3, SWAP70, and ENPP2; IL4R, PPY, SPINT3, COMP, SERPI NG1, APCS and CCL25; IL4R, PPY, SPINT3, COMP, SERPING1, SULT2A1 and KAZN; IL4R, PPY, ZCCHC8, SPINT3, COMP, CCL25 and NTF3; PPY, ZCCHC8, SPINT3, NTF3, F12, IL4R and KAZN; IL4R, PPY, SULT2A1, COMP, F12, SERPING1 and PRAME; PPY, F12, SPINT3, CLIC5, COMP, IL4R and KAZN; IL4R, PPY, SPINT3, COMP, CLIC5, APCS and CCL25;IL4R, PPY, COMP, APCS, IGFBP1, LTA4H and HS3ST3B1; IL4R, PPY, SPINT3, COMP, STX7, SERPING1 and CLIC5; IL4R, PPY, SPINT3, COMP, CLIC5, APCS and KAZN; IL4R, PPY, SPINT3, F12, COMP, APCS and PRAME; PPY, SULT2A1, F12, NTF3, IL4R, APCS and ITPA; PPY, F12, HS3ST3B1, NTF3, SULT2A1, IGFBP1 and KAZN; IL4R, PPY, SPINT3, COMP, CCL25, LTA4H, and NTF3; IL4R, PPY, SPINT3, COMP, F12, LTA4H, and ADGRE5; GIP, F12, HS3ST3B1, NTF3, APCS, NOTCH3, and CCL25; IL4R, SPINT3, CLIC5, COMP, ITPA, APCS, and SOST; IL4R, PPY, CCL25, STX7, ENPP2, SERPING1, and TEX101; PPY, F12, SULT2A1, IGFBP1, NTF3, IL4R, and PRAME; IL4R, PPY, SPINT3, C OMP, F12, APCS and CASP7; IL4R, PPY, SPINT3, COMP, F12, CCL25 and KAZN; IL4R, PPY, SPINT3, COMP, F12, LTA4H and SOST; GIP, F12, HS3ST3B1, NTF3, APCS, NOTCH3 and PRAME; IL4R, PPY, SPINT3, COMP, CLIC5, SERPING1 and PRAME; IL4R, PPY, SPINT3, COMP, F12, CABP2 and NTF3; IL4R, PPY, ZCCHC8, SPINT3, COMP, CCL25 and HS3ST3B1; IL4R, PPY, SPINT3, F12, COMP, SERPING1, and LTA4H; IL4R, PPY, SPINT3, COMP, ITPA, FCER2, and ENPP2; IL4R, PPY, SPINT3, COMP, F12, CCL25, and ADGRE5; IL4R, PPY, SULT2A1, COMP, F12, SERPING1, and LTA4H; IL4R, PPY, SULT2A1, NFASC, CCL25, APCS, and KAZN; PPY, SULT2A1, F12, NTF3, IL4R, APCS, and ADGRE5;IL4R, SPINT3, CLIC5, COMP, PPY, FCER2, and PRAME; IL4R, PPY, SULT2A1, COMP, F12, APCS, and TEX101; IL4R, PPY, SPINT3, COMP, STX7, LTA4H, and CLIC5; IL4R, PPY, SPINT3, F12, COMP, PRAME, and NTF3; IL4R, PPY, COMP, APCS, IGFBP1, FCER2, and STX7; IL4R, PPY, SPINT3, F12, COMP, FCER2, and PRAME; IL4R, PPY, CCL25, STX7, NOTCH3, IGFBP1, and SOST; IL4R, PPY, SPINT3, COMP, SERPING1, APCS, and LTA4H; or IL4R, PPY, COMP, APCS, IGFBP1, ENPP2, and KAZN. ;

[0048] Preferably, the 7 marker combination is: IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1 and ADGRE5; PPY, SULT2A1, F12, NTF3, IL4R, SWAP70 and LTA4H; IL4R, PPY, LTA4H, STX7, SULT2A1, SERPING1 and HS3ST3B1; IL4R, PPY, SULT2A1, COMP, SERPING1, APCS and SOST; PPY, CCL25, STX7, NFASC, LSM1, SERPING1 and APCS; IL4R, PPY, SPINT3, COMP, S TX7, SERPING1, and ENPP2; IL4R, PPY, SPINT3, COMP, F12, LTA4H, and HS3ST3B1; PPY, CCL25, STX7, NFASC, LSM1, ENPP2, and NOTCH3; IL4R, PPY, SULT2A1, COMP, F12, SERPING1, and KAZN; IL4R, PPY, INPP1, STX7, LTA4H, SERPING1, and KAZN; IL4R, PPY, SPINT3, COMP, CLIC5, APCS, and LTA4H; PPY, F12, SULT2A1, IGFBP1, and NTF 3. IL4R and ENPP2; IL4R, PPY, SPINT3, F12, COMP, PRAME and TEX101; IL4R, PPY, SPINT3, COMP, CLIC5, SERPING1 and TEX101; IL4R, PPY, SULT2A1, NTF3, F12, APCS and TEX101; IL4R, PPY, CCL25, STX7, NFASC, YJU2 and ITPA; IL4R, PPY, SPINT3, COMP, STX7, LTA4H and CCL25; IL4R, PPY, CCL25, STX7, ENPP2, CLIC5 and KAZN ;IL4R, PPY, SPINT3, COMP, SERPING1, APCS and ITPA;IL4R, PPY, LTA4H, STX7, NOTCH3, ENPP2 and CCL25;IL4R, PPY, LTA4H, STX7, NOTCH3, ENPP2 and CCL25;IL4R, PPY, SPINT3, COMP, F12, SULT2A1 and HS3ST3B1;IL4R, PPY, SPINT3, COMP, F12, SULT2A1 and HS3ST3B1;PPY, CCL25, STX7, NFASC, IL4R, SERPING1 and APCS;GIP, F12, HS3ST3B1, ADGRE1, NTF3, APCS, and KAZN; PPY, F12, SPINT3, CLIC5, COMP, IL4R, and NTF3; IL4R, PPY, SULT2A1, NTF3, F12, LTA4H, and HS3ST3B1; IL4R, PPY, SPINT3, COMP, F12, CCL25, and TEX101; GIP, F12, HS3ST3B1, ADGRE1, NTF3, YJU2, and PRAME; IL4R, PPY, COMP, APCS, IGFBP1, ENPP2, and ITPA; IL4R, PPY, CCL25, ST X7, ENPP2, SERPING1 and NOTCH3; IL4R, PPY, SPINT3, F12, COMP, SERPING1 and PRAME; IL4R, PPY, INPP1, LTA4H, STX7, PRAME and KAZN; PPY, CCL25, STX7, NFASC, LSM1, FCER2 and APCS; PPY, CCL25, STX7, NFASC, LSM1, F12 and APCS; IL4R, PPY, SPINT3, F12, COMP, APCS and ADGRE5; IL4R, SPINT3, CLIC5, COMP, PPY, FCER2 and KAZN; IL4R, SPINT3, CLIC5, COMP, PPY, APCS and ADGRE5; IL4R, PPY, CCL25, STX7, NFASC, HS3ST3B1 and KAZN; IL4R, PPY, COMP, APCS, IGFBP1, FCER2 and LTA4H; IL4R, PPY, SPINT3, COMP, F12, CABP2 and KAZN; PPY, SULT2A1, F12, NTF3, IL4R, APCS and KAZN; PPY, F12, SPINT3, CLIC5, COMP, APCS and NTF3; IL4R, SPINT3, CLIC5, COMP, PPY, LTA4H and HS3ST3B1; IL4R, PPY, SPINT3, COMP, SERPING1, SULT2A1 and APCS; IL4R, PPY, SPINT3, COMP, STX7, APCS and CLIC5; IL4R, PPY, SPINT3, F12, NTF3, LTA4H and CCL25; PPY, ZCCHC8, SPINT3, NTF3, F12, IL4R and LTA4H; IL4R, PPY, SPINT3, COMP, ITPA, FCER2 and LTA4H; IL4R, PPY, INPP1, LTA4H, STX7, CCL25 and KAZN;IL4R, PPY, CCL25, STX7, ENPP2, APCS and ITPA; IL4R, PPY, CCL25, STX7, NOTCH3, IGFBP1 and ENPP2; IL4R, PPY, CCL25, STX7, ENPP2, NOTCH3 and SOST; IL4R, PPY, SPINT3, F12, COMP, FCER2 and NTF3; IL4R, SPINT3, CLIC5, COMP, ITPA, APCS and ADGRE5; PPY, F12, HS3ST3B1, NTF3, SERPING1, LTA4H and CCL25; PPY, CCL25, STX7, NFASC, LSM1, APCS, and SOST; IL4R, PPY, LTA4H, STX7, NOTCH3, GIP, and TEX101; IL4R, PPY, CCL25, STX7, NOTCH3, ENPP2, and HS3ST3B1; IL4R, PPY, LTA4H, STX7, SULT2A1, CCL25, and PRAME; IL4R, PPY, SULT2A1, NTF3, IGFBP1, APCS, and KAZN; PPY, CCL25, STX7, NFASC, LSM1, NOTCH3, and APCS; GIP, F12, HS3ST3B1, ADG RE1, NTF3, SWAP70, and ITPA; GIP, F12, HS3ST3B1, ADGRE1, NTF3, IL4R, and ENPP2; PPY, ZCCHC8, SPINT3, NTF3, F12, ENPP2, and SOST; PPY, F12, HS3ST3B1, NTF3, SERPING1, IL4R, and CCL25; IL4R, PPY, COMP, APCS, IGFBP1, LTA4H, and SOST; IL4R, PPY, COMP, APCS, IGFBP1, ADGRE5, and CCL25; IL4R, SPINT3, CLIC5, COMP, PPY , SULT2A1 and ITPA; IL4R, PPY, SPINT3, COMP, STX7, PRAME and ENPP2; PPY, F12, HS3ST3B1, NTF3, SULT2A1, SERPING1 and SOST; PPY, CCL25, STX7, NFASC, LSM1, ADGRE5 and APCS; PPY, CCL25, STX7, NFASC, LSM1, LTA4H and ITPA; IL4R, PPY, SPINT3, COMP, F12, FCER2 and LTA4H; IL4R, PPY, CCL25, STX7, LPL, LTA4H and ITPA;PPY, CCL25, STX7, NFASC, IL4R, ENPP2 and KAZN; IL4R, PPY, ZCCHC8, SPINT3, COMP, CCL25 and ITPA; IL4R, PPY, SPINT3, COMP, F12, APCS and SULT2A1; IL4R, PPY, CCL25, STX7, ENPP2, LTA4H and KAZN; PPY, F12, HS3ST3B1, NTF3, SERPING1, NOTCH3 and ITPA; IL4R, PPY, LTA4H, STX7, SULT2A1, ADGRE5 and KAZN; PPY, F12, HS3ST3B1, NTF3, SULT2A1, CABP2 and ENPP2; GIP, F12, HS3ST3B1, NTF3, ADGRE1, LTA4H and KAZN; IL4R, PPY, SPINT3, COMP, CLIC5, FCRL1 and HS3ST3B1; GIP, F 12. HS3ST3B1, ADGRE1, NTF3, SERPING1 and LTA4H; IL4R, PPY, SPINT3, COMP, CCL25, LTA4H and ITPA; IL4R, PPY, LTA4H, STX7, NOTCH3, ADGRE5 and PRAME; IL4R , PPY, SULT2A1, COMP, SERPING1, FCRL1 and ENPP2; IL4R, PPY, COMP, APCS, IGFBP1, SWAP70 and CLIC5; IL4R, PPY, SPINT3, COMP, CCL25, SULT2A1 and LTA4H; IL4R, PPY, CCL25, STX7, NFASC, ENPP2 and APCS; PPY, F12, SULT2A1, IGFBP1, NTF3, SWAP70 and ADGRE5; IL4R, PPY, SULT2A1, COMP, SERPING1, FCER2 and ENPP2; I L4R, PPY, CCL25, STX7, NOTCH3, LTA4H and HS3ST3B1; PPY, F12, HS3ST3B1, NTF3, SULT2A1, IGFBP1 and APCS; IL4R, PPY, SULT2A1, COMP, SPINT3, APCS and LTA4 H; GIP, F12, HS3ST3B1, NTF3, APCS, NOTCH3 and ITPA; GIP, F12, HS3ST3B1, NTF3, ZCCHC8, YJU2 and ITPA; PPY, F12, HS3ST3B1, NTF3, SERPING1, YJU2 and CCL25;IL4R, PPY, CCL25, STX7, NFASC, LTA4H and KAZN; IL4R, PPY, CCL25, NFASC, SULT2A1, APCS and LTA4H; PPY, CCL25, STX7, NFASC, IL4R, HS3ST3B1 and APCS; GIP, AD GRE5, HS3ST3B1, APCS, CCL25, FCRL1 and PRAME; GIP, F12, HS3ST3B1, ADGRE1, NTF3, ENPP2 and SULT2A1; IL4R, PPY, SULT2A1, NTF3, ZCCHC8, APCS and LTA4H; PPY, F12, SPINT3, CLIC5, COMP, CABP2, and NTF3; PPY, SULT2A1, CCL25, NFASC, IL4R, APCS, and HS3ST3B1; IL4R, PPY, CCL25, STX7, ENPP2, GIP, and TEX101; IL4R, PPY, SULT2A1, COMP, F12, APCS, and ADGRE5; IL4R, PPY, SULT2A1, NFASC, CCL25, APCS, and ADGRE5; IL4R, PPY, SULT2A1, NTF3, STX7, CABP2, and HS3ST3B1; PPY, F12, SULT2A 1, IGFBP1, NTF3, HS3ST3B1, and ENPP2; PPY, F12, SULT2A1, IGFBP1, NTF3, YJU2, and CCL25; IL4R, PPY, SULT2A1, NTF3, SERPING1, BLNK, and ADGRE5; PPY, F12, SPINT3, CLIC5, COMP, YJU2, and KAZN; IL4R, PPY, SPINT3, COMP, ITPA, FCRL1, and ENPP2; PPY, CCL25, STX7, NFASC, IL4R, PRAME, and APCS; IL4R, PPY, SPINT3, COMP, ITPA , APCS and LTA4H; IL4R, PPY, SPINT3, COMP, CLIC5, LTA4H and PRAME; IL4R, PPY, SPINT3, F12, NTF3, YJU2 and HS3ST3B1; IL4R, PPY, COMP, APCS, IGFBP1, YJU2 and KAZN; IL4R, PPY, SULT2A1, COMP, F12, LTA4H and NTF3; PPY, F12, SULT2A1, IGFBP1, NTF3, NOTCH3 and ITPA; SWAP70, IGFBP1, STX7, ITPA, SPINT3, SERPING1 and SOST;PPY, SULT2A1, NFASC, NTF3, IL4R, APCS and HS3ST3B1; PPY, CCL25, STX7, NFASC, IL4R, LTA4H and PRAME; PPY, F12, SPINT3, CLIC5, COMP, SERPING1 and TEX101; IL4R, PPY, SPINT3, COMP, CCL25, APCS and ADGRE5; IL4R, SPINT3, CLIC5, COMP, PPY, FCRL1 and KAZN; PPY, F12, SPINT3, CLIC5, COMP, LTA4H and TEX101; PPY, F12, SULT2A1, IGFBP1, NTF3, ADGRE5 and PRAME; PPY, CCL25, STX7, NFASC, LSM1, SULT2A1 and ENPP2; PPY, F12, SULT2A1, IGFBP1, NTF3, LTA4H and HS3ST3B1; PPY, F12, HS3ST3B1, NTF3, SERPING1, ADGRE5 and SOST; IL4R, PPY, INPP1, STX7, LTA4H, CCL25 and APCS; IL4R, PPY, SPINT3, COMP, STX7, CABP2 and HS3ST3B1; IL4R, PPY, CCL25, STX7, NFASC, ADGRE5 and HS3ST3B1; that is, any one of the above 137 7-marker combinations.

[0049] Preferably, the A includes a combination of 8 markers, and the combination of 8 markers is any eight of the combinations in the B.

[0050] Preferably, the 8 markers are combined into d1) and d2):

[0051] d1) the 7 markers combination (preferably any one of the 137 7 markers combinations mentioned above);

[0052] d2) any one of the above B;

[0053] The d2) is different from any one marker in the combination of 7 markers in d1).

[0054] Preferably, the 8 marker combination is: IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, ADGRE5 and LSM1; IL4R, PPY, CCL25, STX7, NFASC, ENPP2, APCS and LSM1; IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, ADGRE5 and CABP2; IL4R, PPY, LTA4H, STX7, NOTCH3, ENPP2, CCL25 and LSM1; PPY, CCL25, STX7, NFASC, IL4R, ENPP2, KAZN and LSM1; IL4R , PPY, CCL25, STX7, NOTCH3, IGFBP1, ENPP2, and LSM1; IL4R, PPY, CCL25, STX7, ENPP2, SERPING1, NOTCH3, and LSM1; GIP, F12, HS3ST3B1, NTF3, ADGRE1, LTA4H, KAZN, and LSM1; IL4R, PPY, SPINT3, COMP, STX7, SERPING1, ENPP2, and LSM1; PPY, CCL25, STX7, NFASC, IL4R, LTA4H, PRAME, and LSM1; PPY, F12, HS3ST3B1, N TF3, SERPING1, ADGRE5, SOST and KAZN; GIP, F12, HS3ST3B1, ADGRE1, NTF3, APCS, KAZN and LSM1; GIP, F12, HS3ST3B1, ADGRE1, NTF3, APCS, KAZN and CABP2; P PY, F12, SULT2A1, IGFBP1, NTF3, LTA4H, HS3ST3B1 and LSM1; IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, ADGRE5 and INPP1; IL4R, PPY, SPINT3, F12, NTF3 , LTA4H, CCL25 and STX7; IL4R, PPY, SPINT3, COMP, CLIC5, APCS, LTA4H and ADGRE5; IL4R, PPY, CCL25, STX7, NOTCH3, IGFBP1, ENPP2 and CABP2; IL4R, PPY, SULT2A1, COMP, F12, LTA4H, NTF3 and LSM1; PPY, CCL25, STX7, NFASC, IL4R, PRAME, APCS and LSM1; PPY, F12, HS3ST3B1, NTF3, SERPING1, ADGRE5, SOST and LSM1;PPY, CCL25, STX7, NFASC, LSM1, F12, APCS, and FCRL1; IL4R, PPY, SPINT3, COMP, CLIC5, SERPING1, TEX101, and STX7; PPY, CCL25, STX7, NFASC, LSM1, ADGRE5, APCS, and CABP2; PPY, SULT2A1, F12, NTF3, IL4R, SWAP70, LTA4H, and LSM1; PPY, F12, HS3ST3B1, NTF3, SERPING1, NOTCH3, ITPA, and LSM1; PPY, F12, SPINT3, CLIC5, CO MP, IL4R, NTF3 and CABP2; PPY, F12, SULT2A1, IGFBP1, NTF3, IL4R, ENPP2 and LSM1; PPY, CCL25, STX7, NFASC, IL4R, HS3ST3B1, APCS and LSM1; PPY, F12, SPINT3, CLIC5, COMP, IL4R, NTF3 and LSM1; IL4R, PPY, CCL25, STX7, NOTCH3, IGFBP1, ENPP2 and INPP1; PPY, SULT2A1, NFASC, NTF3, IL4R, APCS, HS3ST3B1 and CABP2; GIP, F1 2, HS3ST3B1, ADGRE1, NTF3, SERPING1, LTA4H and LSM1; PPY, SULT2A1, NFASC, NTF3, IL4R, APCS, HS3ST3B1 and LSM1; IL4R, PPY, LTA4H, STX7, NOTCH3, ENPP2, CCL25 and FASLG; IL4R, PPY, SPINT3, F12, NTF3, YJU2, HS3ST3B1 and SOST; IL4R, PPY, COMP, APCS, IGFBP1, ADGRE5, CCL25 and LSM1; IL4R, PPY, CCL25, STX7, ENPP2, SERPING1, NOTCH3 and INPP1; IL4R, PPY, SPINT3, F12, NTF3, YJU2, HS3ST3B1 and STX7; IL4R, PPY, SPINT3, F12, NTF3, YJU2, HS3ST3B1 and ADGRE1; IL4R, SPINT3, CLIC5, COMP, PPY, APCS, ADGRE5 and CASP7; IL4R, SPINT3, CLIC5, COMP, PPY, APCS, ADGRE5 and LSM1; GIP, F12, HS3ST3B1, ADGRE1, NTF3, ENPP2, SULT2A1 and LSM1;IL4R, PPY, CCL25, STX7, NFASC, LTA4H, KAZN and INPP1; GIP, ADGRE5, HS3ST3B1, APCS, CCL25, FCRL1, PRAME and LSM1; PPY, F12, SPINT3, CLIC5, COMP, IL4R, N TF3 and SWAP70; PPY, F12, HS3ST3B1, NTF3, SULT2A1, SERPING1, SOST and LSM1; IL4R, PPY, CCL25, STX7, NOTCH3, LTA4H, HS3ST3B1 and LSM1; PPY, F12, SPINT3, CL IC5, COMP, IL4R, NTF3, and ADGRE5; IL4R, PPY, SPINT3, COMP, ITPA, APCS, LTA4H, and LSM1; IL4R, PPY, CCL25, NFASC, SULT2A1, APCS, LTA4H, and LSM1; IL4R, PPY, SULT2A1, COMP, F12, LTA4H, NTF3, and KAZN; IL4R, PPY, SULT2A1, NTF3, IGFBP1, APCS, KAZN, and LSM1; IL4R, PPY, SPINT3, COMP, STX7, APCS, CLIC5, and LSM1; IL4R, PP Y, SPINT3, F12, NTF3, YJU2, HS3ST3B1, and ADGRE5; GIP, F12, HS3ST3B1, NTF3, ADGRE1, LTA4H, KAZN, and CABP2; PPY, F12, SULT2A1, IGFBP1, NTF3, IL4R, ENPP2, and CABP2; IL4R, PPY, CCL25, STX7, NFASC, ENPP2, APCS, and CABP2; IL4R, PPY, SULT2A1, COMP, F12, APCS, ADGRE5, and LSM1; IL4R, PPY, SULT2A1, NFASC, CCL25, AP CS, ADGRE5 and LSM1; PPY, F12, HS3ST3B1, NTF3, SULT2A1, SERPING1, SOST and SWAP70; IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, ADGRE5 and FCRL1; IL4R, PPY, SPINT3, COMP, CLIC5, APCS, LTA4H and LSM1; PPY, CCL25, STX7, NFASC, IL4R, SERPING1, APCS and LSM1; GIP, F12, HS3ST3B1, NTF3, APCS, NOTCH3, ITPA and CABP2;IL4R, PPY, SPINT3, F12, COMP, SERPING1, PRAME and AFM; PPY, F12, SPINT3, CLIC5, COMP, IL4R, NTF3 and INPP1; PPY, CCL25, STX7, NFASC, LSM1, NOTCH3, APCS with FASLG; PPY, F12, SULT2A1, IGFBP1, NTF3, SWAP70, ADGRE5, and STX7; IL4R, SPINT3, CLIC5, COMP, PPY, SULT2A1, ITPA, and LSM1; IL4R, PPY, SULT2A1, NTF3, F12 , LTA4H, HS3ST3B1 and LSM1; GIP, ADGRE5, HS3ST3B1, APCS, CCL25, FCRL1, PRAME and SOD3; PPY, CCL25, STX7, NFASC, IL4R, HS3ST3B1, APCS and CABP2; PPY, CCL25 , STX7, NFASC, LSM1, NOTCH3, APCS and CABP2; IL4R, PPY, CCL25, STX7, ENPP2, SERPING1, NOTCH3 and CABP2; PPY, F12, HS3ST3B1, NTF3, SERPING1, ADGRE5, SOS T and FASLG; IL4R, PPY, SPINT3, F12, COMP, FCER2, NTF3 and LSM1; PPY, SULT2A1, CCL25, NFASC, IL4R, APCS, HS3ST3B1 and LSM1; IL4R, PPY, SPINT3, COMP, CCL25, LTA4H, ITPA and NTF3; PPY, F12, SPINT3, CLIC5, COMP, APCS, NTF3 and STX7; IL4R, PPY, COMP, APCS, IGFBP1, SWAP70, CLIC5 and AFM; PPY, F12, SPINT3, CLIC5, COM P, CABP2, NTF3 and FCRL1; PPY, F12, HS3ST3B1, NTF3, SERPING1, ADGRE5, SOST and CABP2; IL4R, PPY, SPINT3, F12, COMP, SERPING1, PRAME and INPP1; GIP, F12, HS3ST3B1, ADGRE1, NTF3, ENPP2, SULT2A1 and CABP2; IL4R, PPY, SULT2A1, COMP, F12, LTA4H, NTF3 and CABP2; IL4R, PPY, SPINT3, COMP, F12, FCER2, LTA4H and STX7;IL4R, PPY, SPINT3, COMP, STX7, SERPING1, ENPP2 and F12; IL4R, SPINT3, CLIC5, COMP, PPY, APCS, ADGRE5 and CABP2; IL4R, PPY, SPINT3, COMP, F12, LTA4H , HS3ST3B1 and ADGRE5; IL4R, PPY, CCL25, STX7, NOTCH3, ENPP2, HS3ST3B1 and LSM1; IL4R, PPY, SPINT3, F12, NTF3, LTA4H, CCL25 and ZCCHC8; IL4R, PPY, CCL 25, STX7, ENPP2, NOTCH3, SOST, and LSM1; PPY, SULT2A1, NFASC, NTF3, IL4R, APCS, HS3ST3B1, and FCRL1; PPY, ZCCHC8, SPINT3, NTF3, F12, IL4R, LTA4H, and LSM1; IL4R, PPY, CCL25, STX7, NFASC, HS3ST3B1, KAZN, and LSM1; PPY, CCL25, STX7, NFASC, IL4R, SERPING1, APCS, and CABP2; IL4R, PPY, CCL25, NFASC, SULT2A1 , APCS, LTA4H and CABP2; PPY, CCL25, STX7, NFASC, LSM1, ADGRE5, APCS and FASLG; PPY, F12, SPINT3, CLIC5, COMP, IL4R, NTF3 and ADGRE1; PPY, F12, HS3ST3B1, NTF3, SULT2A1, CABP2, ENPP2 and LSM1; GIP, F12, HS3ST3B1, ADGRE1, NTF3, SWAP70, ITPA and LSM1; IL4R, PPY, SULT2A1, NTF3, F12, LTA4H, HS3ST3B1 and C ABP2; GIP, F12, HS3ST3B1, ADGRE1, NTF3, SWAP70, ITPA, and CABP2; IL4R, PPY, SULT2A1, NTF3, STX7, CABP2, HS3ST3B1, and ZCCHC8; PPY, CCL25, STX7, NFASC, LSM1, ADGRE5, APCS, and INPP1; IL4R, PPY, SULT2A1, COMP, F12, SERPING1, KAZN, and INPP1; PPY, F12, HS3ST3B1, NTF3, SERPING1, ADGRE5, SOST, and SWAP70;GIP, F12, HS3ST3B1, ADGRE1, NTF3, APCS, KAZN and SOD3; PPY, SULT2A1, CCL25, NFASC, IL4R, APCS, HS3ST3B1 and KAZN; PPY, CCL25, STX7, NFASC, LSM1, SERPING1, APCS and CABP2; GIP, F12, HS3ST3B1, ADGRE1, NTF3, SERPING1, LTA4H and KAZN; PPY, F12, SPINT3, CLIC5, COMP, CABP2, NTF3 and STX7; IL4R, PPY, SPINT3, F 12, COMP, SERPING1, PRAME and CABP2; PPY, F12, SPINT3, CLIC5, COMP, YJU2, KAZN and SOST; IL4R, PPY, SPINT3, COMP, CCL25, APCS, ADGRE5 and STX7; PPY, SULT2A1, F12, NTF3, IL4R, SWAP70, LTA4H and CABP2; GIP, F12, HS3ST3B1, NTF3, APCS, NOTCH3, ITPA and LSM1; PPY, F12, SULT2A1, IGFBP1, NTF3, SWAP70, ADGRE5 and L SM1; GIP, F12, HS3ST3B1, ADGRE1, NTF3, YJU2, PRAME, and LSM1; PPY, CCL25, STX7, NFASC, LSM1, NOTCH3, APCS, and FCRL1; PPY, F12, SULT2A1, IGFBP1, NTF3, LTA4H, HS3ST3B1, and STX7; IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, ADGRE5, and ITPA; IL4R, PPY, SPINT3, COMP, STX7, PRAME, ENPP2, and F12; IL4R, PPY, SULT2A 1, COMP, F12, LTA4H, NTF3 and ZCCHC8; IL4R, PPY, CCL25, NFASC, SULT2A1, APCS, LTA4H and INPP1; IL4R, PPY, SPINT3, F12, NTF3, LTA4H, CCL25 and AFM; GIP, F12, HS3ST3B1, NTF3, APCS, NOTCH3, ITPA and PPY; PPY, F12, SPINT3, CLIC5, COMP, APCS, NTF3 and ADGRE5; PPY, CCL25, STX7, NFASC, LSM1, SERPING1, APCS and SOD3;IL4R, PPY, COMP, APCS, IGFBP1, SWAP70, CLIC5 and LSM1; IL4R, PPY, SULT2A1, NTF3, F12, LTA4H, HS3ST3B1 and ZCCHC8; IL4R, PPY, COMP, APCS, IGFBP1, FCER2, L TA4H and LSM1; PPY, SULT2A1, CCL25, NFASC, IL4R, APCS, HS3ST3B1 and CABP2; PPY, ZCCHC8, SPINT3, NTF3, F12, IL4R, LTA4H and STX7; PPY, F12, SPINT3, CLIC5, CO MP, APCS, NTF3 and CABP2; PPY, F12, SPINT3, CLIC5, COMP, CABP2, NTF3 and SOST; PPY, CCL25, STX7, NFASC, IL4R, SERPING1, APCS and FCRL1; IL4R, PPY, SULT2A1, COMP, F12, LTA4H, NTF3 and INPP1; IL4R, PPY, SPINT3, COMP, CCL25, APCS, ADGRE5 and LSM1; PPY, SULT2A1, NFASC, NTF3, IL4R, APCS, HS3ST3B1 and ADGRE5; IL4R, SP INT3, CLIC5, COMP, PPY, FCRL1, KAZN, and ADGRE1; PPY, CCL25, STX7, NFASC, LSM1, NOTCH3, APCS, and SWAP70; PPY, F12, HS3ST3B1, NTF3, SERPING1, NOTCH3, ITPA, and CABP2; PPY, F12, HS3ST3B1, NTF3, SULT2A1, IGFBP1, APCS, and LSM1; IL4R, PPY, CCL25, STX7, ENPP2, SERPING1, NOTCH3, and KAZN; IL4R, PPY, SPINT3, COMP, F12 , SULT2A1, HS3ST3B1 and ADGRE1; PPY, SULT2A1, CCL25, NFASC, IL4R, APCS, HS3ST3B1 and INPP1; GIP, F12, HS3ST3B1, NTF3, APCS, NOTCH3, ITPA and KAZN; GIP, F12, HS3ST3B1, ADGRE1, NTF3, YJU2, PRAME and SOST; IL4R, PPY, LTA4H, STX7, NOTCH3, ENPP2, CCL25 and CABP2; PPY, F12, SPINT3, CLIC5, COMP, APCS, NTF3 and ADGRE1;IL4R, PPY, SULT2A1, COMP, F12, LTA4H, NTF3 and ITPA; IL4R, PPY, SPINT3, COMP, STX7, APCS, CLIC5 and CABP2; IL4R, SPINT3, CLIC5, COMP, PPY, FCRL1, KAZN and ADGRE5; IL4R, SPINT3, CLIC5, COMP, PPY, FCRL1, KAZN and ADGRE5; IL4R, SPINT3, CLIC5, COMP, PPY, SULT2A1, ITPA and INPP1; IL4R, PPY, SULT2A1, NTF3, ZC CHC8, APCS, LTA4H and CABP2; IL4R, PPY, COMP, APCS, IGFBP1, SWAP70, CLIC5 and STX7; PPY, F12, SPINT3, CLIC5, COMP, IL4R, NTF3 and ITPA; IL4R, PPY, SPINT3, COMP, ITPA, FCRL1, ENPP2 and ADGRE5; IL4R, SPINT3, CLIC5, COMP, PPY, SULT2A1, ITPA and STX7; PPY, ZCCHC8, SPINT3, NTF3, F12, ENPP2, SOST and STX7; IL4R, PPY, SPINT3, F12, COMP, APCS, ADGRE5 and LSM1; IL4R, PPY, SPINT3, COMP, SERPING1, SULT2A1, APCS and LSM1; IL4R, PPY, SPINT3, COMP, STX7, APCS, CLIC5 and AFM; PPY, CCL25, STX7, NFASC, LSM1, NOTCH3, APCS and INPP1; IL4R, SPINT3, CLIC5, COMP, PPY, LTA4H, HS3ST3B1 and STX7; SWAP70, IGFBP1, STX7, ITPA, SPIN T3, SERPING1, SOST and PPY; PPY, F12, SPINT3, CLIC5, COMP, YJU2, KAZN and STX7; IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, ADGRE5 and AFM; IL4R, PPY, SPINT3, COMP, F12, CABP2, KAZN and ADGRE5; IL4R, PPY, SPINT3, F12, COMP, SERPING1, PRAME and ITPA; PPY, F12, SULT2A1, IGFBP1, NTF3, LTA4H, HS3ST3B1 and INPP1;PPY, SULT2A1, CCL25, NFASC, IL4R, APCS, HS3ST3B1 and SOST; IL4R, PPY, SPINT3, COMP, STX7, APCS, CLIC5 and F12; IL4R, PPY, ZCCHC8, SPINT3, COMP, CC L25, ITPA and ADGRE5; IL4R, PPY, SULT2A1, COMP, F12, LTA4H, NTF3 and AFM; PPY, SULT2A1, NFASC, NTF3, IL4R, APCS, HS3ST3B1 and ADGRE1; IL4R, PPY, CCL25 , STX7, ENPP2, LTA4H, KAZN and F12; PPY, CCL25, STX7, NFASC, IL4R, ENPP2, KAZN and CABP2; IL4R, PPY, SPINT3, COMP, STX7, APCS, CLIC5 and ADGRE5; IL4R, PPY, CCL25, STX7, ENPP2, SERPING1, NOTCH3 and FCRL1; GIP, F12, HS3ST3B1, ADGRE1, NTF3, SWAP70, ITPA and ADGRE5; IL4R, PPY, CCL25, STX7, NFASC, LTA 4H, KAZN and LSM1; PPY, F12, SPINT3, CLIC5, COMP, LTA4H, TEX101 and STX7; IL4R, PPY, CCL25, STX7, NFASC, ADGRE5, HS3ST3B1 and LSM1; PPY, CCL25, STX7, NFASC, IL4R, HS3ST3B1, APCS and FCRL1; GIP, F12, HS3ST3B1, ADGRE1, NTF3, SERPING1, LTA4H and SOST; IL4R, PPY, SPINT3, COMP, SERPING1, SULT2A1, AP CS and FCRL1; IL4R, PPY, COMP, APCS, IGFBP1, FCER2, LTA4H and INPP1; IL4R, PPY, SULT2A1, COMP, F12, LTA4H, NTF3 and ADGRE1; IL4R, PPY, LTA4H, STX7, NOTCH3, ENPP2, CCL25 and FCRL1; IL4R, PPY, SULT2A1, NTF3, STX7, CABP2, HS3ST3B1 and SERPING1; PPY, CCL25, STX7, NFASC, LSM1, SERPING1, APCS and INPP1;PPY, CCL25, STX7, NFASC, LSM1, LTA4H, ITPA, and SERPING1; IL4R, PPY, SPINT3, F12, NTF3, LTA4H, CCL25, and ADGRE1; IL4R, PPY, INPP1, STX7, LTA4H, SERPING1, KAZN, and LSM1; PPY, F12, SULT2A1, IGFBP1, NTF3, YJU2, CCL25, and ADGRE1; IL4R, PPY, SPINT3, COMP, CCL25, APCS, ADGRE5, and CABP2; IL4R, PPY, COMP, and APC S, IGFBP1, SWAP70, CLIC5, and CABP2; IL4R, PPY, SULT2A1, NFASC, CCL25, APCS, ADGRE5, and CABP2; PPY, F12, SULT2A1, IGFBP1, NTF3, HS3ST3B1, ENPP2, and LSM1; IL4R, PPY, SPINT3, COMP, STX7, LTA4H, CCL25, and ADGRE5; IL4R, SPINT3, CLIC5, COMP, PPY, LTA4H, HS3ST3B1, and SOST; PPY, CCL25, STX7, NFASC, IL4R, SE RPING1, APCS and SWAP70; IL4R, PPY, SULT2A1, COMP, SPINT3, APCS, LTA4H and ADGRE1; GIP, F12, HS3ST3B1, NTF3, APCS, NOTCH3, ITPA and LTA4H; GIP, ADGRE5 , HS3ST3B1, APCS, CCL25, FCRL1, PRAME and SOST; IL4R, PPY, CCL25, STX7, NFASC, ADGRE5, HS3ST3B1 and SERPING1; PPY, CCL25, STX7, NFASC, LSM1, SERPING 1. APCS and FASLG; PPY, F12, SPINT3, CLIC5, COMP, IL4R, NTF3 and FASLG; GIP, ADGRE5, HS3ST3B1, APCS, CCL25, FCRL1, PRAME and FASLG; IL4R, PPY, CCL25, STX7, ENPP2, NOTCH3, SOST and CABP2; IL4R, SPINT3, CLIC5, COMP, ITPA, APCS, ADGRE5 and LSM1; GIP, F12, HS3ST3B1, ADGRE1, NTF3, SERPING1, LTA4H and INPP1;IL4R, PPY, SPINT3, COMP, SERPING1, APCS, ITPA, and FASLG; IL4R, PPY, SPINT3, COMP, F12, CCL25, TEX101, and CABP2; IL4R, PPY, SULT2A1, COMP, SERPING1, APCS, SOST, and LSM1; IL4R, PPY, COMP, APCS, IGFBP1, FCER2, LTA4H, and CABP2; GIP, F12, HS3ST3B1, ADGRE1, NTF3, SERPING1, LTA4H, and SWAP70; PPY, SULT2A1, F12 , NTF3, IL4R, APCS, KAZN and LSM1; PPY, F12, SULT2A1, IGFBP1, NTF3, IL4R, ENPP2 and SOD3; IL4R, PPY, SPINT3, COMP, CLIC5, FCRL1, HS3ST3B1 and AFM; IL4R, PPY, SPINT3, F12, COMP, PRAME, TEX101 and ADGRE5; PPY, CCL25, STX7, NFASC, LSM1, SERPING1, APCS and FCRL1; IL4R, PPY, SULT2A1, NTF3, STX7, CABP2, HS3ST3B 1 and SOST; IL4R, PPY, SPINT3, COMP, CLIC5, APCS, LTA4H and STX7; IL4R, PPY, SULT2A1, COMP, F12, LTA4H, NTF3 and FCRL1; IL4R, PPY, SPINT3, COMP, F12, CCL25, TEX101 and ADGRE5; GIP, F12, HS3ST3B1, ADGRE1, NTF3, SWAP70, ITPA and FASLG; IL4R, PPY, SPINT3, COMP, STX7, APCS, CLIC5 and SERPING1; IL4R, PPY, SPINT3, F12, NTF3, LTA4H, CCL25, and SERPING1; IL4R, PPY, SULT2A1, NTF3, ZCCHC8, APCS, LTA4H, and INPP1; IL4R, PPY, SPINT3, COMP, CLIC5, LTA4H, PRAME, and STX7; GIP, F12, HS3ST3B1, NTF3, APCS, NOTCH3, ITPA, and COMP; PPY, CCL25, STX7, NFASC, LSM1, APCS, SOST, and INPP1; PPY, F12, SPINT3, CLIC5, COMP, IL4R, NTF3, and STX7;IL4R, PPY, SPINT3, COMP, CCL25, APCS, ADGRE5 and F12; IL4R, PPY, SULT2A1, NTF3, IGFBP1, APCS, KAZN and ADGRE1; IL4R, PPY, SULT2A1, NTF3, F12, LTA4H, HS3ST3B1 and SOST; SWAP70, IGFBP1, STX7, ITPA, SPINT3, SERPING1, SOST and ADGRE1; PPY, F12, SULT2A1, IGFBP1, NTF3, LTA4H, HS3ST3B1 and ITPA; IL4R, PPY, COMP, APCS, IGFBP1, FCER2, LTA4H, and ADGRE5; IL4R, PPY, SPINT3, COMP, STX7, SERPING1, ENPP2, and ADGRE1; IL4R, PPY, SPINT3, COMP, CLIC5, FCRL1, HS3ST3B1, and STX7; IL4R, PPY, SPINT3, F12, COMP, FCER2, NTF3, and SOST; GIP, F12, HS3ST3B1, ADGRE1, NTF3, APCS, KAZN, and FASLG; IL4R, PPY, SPINT3, COMP, F12, CABP2, KAZN, and SE RPING1; PPY, F12, HS3ST3B1, NTF3, SERPING1, IL4R, CCL25, and SOST; IL4R, PPY, SPINT3, COMP, F12, CCL25, TEX101, and FCRL1; IL4R, PPY, SPINT3, COMP, CCL25, LTA4H, ITPA, and CASP7; PPY, ZCCHC8, SPINT3, NTF3, F12, ENPP2, SOST, and CABP2; PPY, CCL25, STX7, NFASC, LSM1, SERPING1, APCS, and KAZN; IL4R, PPY, SPINT3, COMP, P, F12, CCL25, TEX101 and STX7; IL4R, PPY, SPINT3, F12, COMP, SERPING1, PRAME and LSM1; IL4R, PPY, SPINT3, COMP, CLIC5, APCS, LTA4H and ADGRE1; IL4R, PPY, SU LT2A1, COMP, SPINT3, APCS, LTA4H and CABP2; IL4R, PPY, SPINT3, COMP, CLIC5, APCS, LTA4H and CABP2; IL4R, PPY, SPINT3, COMP, CCL25, APCS, ADGRE5 and ADGRE1;PPY, ZCCHC8, SPINT3, NTF3, F12, IL4R, LTA4H, and ADGRE1; IL4R, SPINT3, CLIC5, COMP, PPY, FCRL1, KAZN, and TEX101; IL4R, PPY, INPP1, LTA4H, STX7, PRAME, KAZN, and LSM1; PPY, CCL25, STX7, NFASC, IL4R, HS3ST3B1, APCS, and SWAP70; IL4R, PPY, CCL25, STX7, ENPP2, SERPING1, NOTCH3, and HS3ST3B1; IL4R, PPY, SULT2A1, NT F3, ZCCHC8, APCS, LTA4H and LSM1; IL4R, PPY, CCL25, STX7, ENPP2, SERPING1, NOTCH3 and ITPA; IL4R, PPY, SPINT3, COMP, CLIC5, LTA4H, PRAME and ADGRE5; IL4R, P PY, SULT2A1, NTF3, F12, LTA4H, HS3ST3B1 and FCRL1; IL4R, PPY, SULT2A1, NTF3, STX7, CABP2, HS3ST3B1 and LSM1; IL4R, PPY, SPINT3, COMP, STX7, SERPING1, EN PP2 and CABP2; IL4R, PPY, SPINT3, COMP, F12, APCS, SULT2A1 and CABP2; IL4R, PPY, CCL25, STX7, NFASC, LTA4H, KAZN and FCRL1; PPY, CCL25, STX7, NFASC, LSM1, FCER2, APCS and FASLG; PPY, CCL25, STX7, NFASC, LSM1, F12, APCS and FASLG; IL4R, PPY, LTA4H, STX7, SULT2A1, SERPING1, HS3ST3B1 and SWAP70; IL4R, SPINT3, CLI C5, COMP, ITPA, APCS, ADGRE5 and CASP7; PPY, F12, SPINT3, CLIC5, COMP, APCS, NTF3 and LSM1; GIP, F12, HS3ST3B1, NTF3, ADGRE1, LTA4H, KAZN and STX7; PPY, CCL25, STX7, NFASC, IL4R, ENPP2, KAZN and FCRL1; PPY, F12, SPINT3, CLIC5, COMP, LTA4H, TEX101 and ADGRE1; IL4R, PPY, SPINT3, F12, NTF3, YJU2, HS3ST3B1 and LSM1;IL4R, PPY, COMP, APCS, IGFBP1, ADGRE5, CCL25, and CABP2; GIP, F12, HS3ST3B1, ADGRE1, NTF3, APCS, KAZN, and SOST; PPY, CCL25, STX7, NFASC, LSM1, SULT2A1, ENPP2, and SWAP70; IL4R, PPY, SPINT3, COMP, STX7, CABP2, HS3ST3B1, and F12; IL4R, PPY, COMP, APCS, IGFBP1, FCER2, LTA4H, and ZCCHC8; PPY, CCL25, STX7 , NFASC, LSM1, SERPING1, APCS and AFM; IL4R, PPY, SPINT3, COMP, F12, LTA4H, HS3ST3B1 and SOST; PPY, ZCCHC8, SPINT3, NTF3, F12, ENPP2, SOST and ADGRE5; IL4R, PPY, SPINT3, F12, COMP, SERPING1, PRAME and FASLG; IL4R, PPY, SPINT3, COMP, F12, APCS, SULT2A1 and ADGRE5; PPY, CCL25, STX7, NFASC, LSM1, ADG RE5, APCS and ADGRE1; IL4R, PPY, SULT2A1, COMP, F12, APCS, ADGRE5 and CABP2; PPY, CCL25, STX7, NFASC, LSM1, ENPP2, NOTCH3 and SERPING1; PPY, CCL25, STX7, NFASC, IL4R, PRAME, APCS and SERPING1; GIP, F12, HS3ST3B1, ADGRE1, NTF3, YJU2, PRAME and CABP2; IL4R, SPINT3, CLIC5, COMP, PPY, SULT2A1, ITPA and CABP2; PPY, F12, HS3ST3B1, NTF3, SERPING1, LTA4H, CCL25, and SOD3; IL4R, PPY, SULT2A1, COMP, F12, SERPING1, KAZN, and SWAP70; IL4R, SPINT3, CLIC5, COMP, ITPA, APCS, ADGRE5, and ENPP2; GIP, F12, HS3ST3B1, NTF3, ADGRE1, LTA4H, KAZN, and INPP1; IL4R, PPY, SPINT3, COMP, STX7, CABP2, HS3ST3B1, and ADGRE1;IL4R, PPY, CCL25, STX7, ENPP2, NOTCH3, SOST, and FASLG; IL4R, PPY, SPINT3, COMP, CCL25, SULT2A1, LTA4H, and ADGRE5; PPY, F12, SULT2A1, IGFBP1, NTF3, HS3ST3B1, ENPP2, and SOST; or IL4R, PPY, SPINT3, COMP, CCL25, LTA4H, ITPA, and ADGRE1.

[0055] Preferably, the A further includes at least one of G, the union of the G and the A (A excluding G) is the B, and the G and the A (A excluding G) have no intersection (for example: the A (A excluding G) includes IL4R, then G is B that only does not include IL4R (that is, the union of G and IL4R is B); the A (A excluding G) includes TMPRSS15, then G is B that only does not include TMPRSS15; the A (A excluding G) includes TMPRSS15 and IL4R, then G is B that only does not include TMPRSS15 and IL4R).

[0056] Preferably, the test sample of the product is selected from at least one of body fluids, tissues, cells, and excretions of the subject to be tested; more preferably, it is body fluid.

[0057] Preferably, the body fluid comprises at least one of blood, lymph, pleural effusion, cerebrospinal fluid, joint fluid, ascites, saliva, lymph, and body fluid; further comprises blood.

[0058] Preferably, the tissue comprises intestinal mucosal tissue.

[0059] Preferably, the blood includes at least one of serum, plasma, dried blood spots, and whole blood; more preferably, it is serum.

[0060] Preferably, the excreta comprises at least one of urine, feces, tears and sweat.

[0061] Preferably, the test sample is selected from the serum of the subject to be tested.

[0062] Preferably, the subject to be tested includes mammals, such as humans, non-human primates (such as gorillas and apes), rodents (such as rats, mice, and guinea pigs), pets (such as cats and dogs), and livestock (such as horses, cows, sheep, pigs, and rabbits).

[0063] Preferably, the subject to be tested includes humans.

[0064] Preferably, the substance for detecting a marker comprises a substance for quantitatively detecting a marker.

[0065] Preferably, the substance for detecting a marker includes a substance for detecting a marker at a gene level and / or a protein level.

[0066] Preferably, the substance comprises a substance for one or more detection techniques or methods selected from the group consisting of immunohistochemistry, Western blotting, Northern blotting, PCR, biochip, nucleic acid sequencing, amino acid sequencing, high performance liquid chromatography, capillary gel electrophoresis, near infrared spectroscopy, mass spectrometry, surface plasmon resonance technology, immuno-PCR technology, and biotin-avidin technology.

[0067] Preferably, the immunohistochemistry method comprises at least one of enzyme-linked immunosorbent assay, immunofluorescence assay, radioimmunoassay, immunocoprecipitation, immunochemiluminescence, colloidal gold immunoassay, fluorescence immunochromatography, complement fixation analysis, flow cytometry fluorescence resolution, and single molecule detection technology; further comprises enzyme-linked immunosorbent assay; and further comprises double antibody sandwich method.

[0068] Preferably, the substance for detecting the marker is selected from one or more of: a substance specific to the marker, a marker-specific probe, a gene chip, a protein chip, and a PCR primer.

[0069] Preferably, the substance specific for the marker comprises any one of h1) to h4):

[0070] h1) an antibody that specifically binds to the marker;

[0071] h2) a ligand protein or polypeptide that specifically binds to the marker;

[0072] h3) non-protein compounds that specifically recognize markers;

[0073] h4) Nucleic acid aptamers that specifically recognize markers.

[0074] Preferably, the antibody comprises at least one of a polyclonal antibody, a monoclonal antibody, a single-chain antibody, a functional antibody fragment, an antibody Fab region, a nanobody, a chimeric antibody, and a multispecific antibody.

[0075] Preferably, the product comprises at least one of a reagent, a kit, a test paper, a chip, a device, and a system.

[0076] Preferably, the product also contains substances for detecting other markers used for screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas, for example, the markers may be TOP1, C19ORF43, etc. disclosed in CN111455053A.

[0077] Preferably, the product further comprises auxiliary detection reagents for gene expression levels, and the auxiliary detection reagents for gene expression levels include: a reaction reagent for visualizing the amplicon corresponding to the primer, an RNA extraction reagent, a reverse transcription reagent, a cDNA amplification reagent, a standard substance for preparing a standard curve, and at least one of a positive control substance.

[0078] Preferably, the product further comprises an auxiliary detection reagent for protein expression, and the auxiliary detection reagent for protein expression comprises at least one of a color developer, a blocking solution, an antibody diluent, a washing buffer, a color stop solution, a standard substance for preparing a standard curve, and a positive control substance.

[0079] Preferably, the colorectal adenoma comprises colorectal advanced adenoma and / or colorectal non-advanced adenoma; more preferably, it is colorectal advanced adenoma.

[0080] Preferably, the diagnosis is to distinguish between a subject suffering from colorectal adenoma and a healthy subject.

[0081] In the second aspect of the present invention, a marker combination is provided, which comprises at least two of B described in the first aspect of the present invention, and can be 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, or 45 of B.

[0082] Preferably, the marker combination comprises:

[0083] e1)TMPRSS15; and

[0084] e2) any one of the above B;

[0085] Any one of the markers in e2) is different from that in e1).

[0086] Preferably, the marker combination comprises:

[0087] f1)IL4R; and

[0088] f2) any one of the above B;

[0089] Any marker in f2) is different from that in f1).

[0090] Preferably, the marker combination comprises:

[0091] g1) TMPRSS15 and IL4R.

[0092] Preferably, the marker combination further comprises at least one of J, the union of J and g1) is B, and J has no intersection with g1).

[0093] Preferably, the marker combination comprises any 2, 3, 4, 5, 6, 7, or 8 of the Bs.

[0094] Preferably, the marker combination is a 2-markers combination, a 3-markers combination, a 4-markers combination, a 5-markers combination, a 6-markers combination, a 7-markers combination or an 8-markers combination in the first aspect of the present invention.

[0095] Preferably, the marker combination is used for screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenoma.

[0096] Preferably, the marker combination is derived from at least one of body fluids, tissues, cells, and excretions of the subject to be tested; more preferably, body fluids.

[0097] Preferably, the subject to be detected, body fluid, tissue, cell, or excrement is the subject to be detected, body fluid, tissue, cell, or excrement in the first aspect of the present invention.

[0098] Preferably, the colorectal adenoma comprises colorectal advanced adenoma and / or colorectal non-advanced adenoma; more preferably, it is colorectal advanced adenoma.

[0099] Preferably, the diagnosis is to distinguish between a subject suffering from colorectal adenoma and a healthy subject.

[0100] The third aspect of the present invention is to provide a product comprising substances for detecting the marker combination of the second aspect of the present invention.

[0101] Preferably, the product is used for screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas.

[0102] Preferably, the substance for detecting the marker combination comprises a substance for quantitatively detecting the marker combination.

[0103] Preferably, the substance for detecting the marker combination comprises a substance for detecting the marker combination at the gene level and / or protein level.

[0104] Preferably, the substance comprises a substance for one or more detection techniques or methods selected from the group consisting of immunohistochemistry, Western blotting, Northern blotting, PCR, biochip, nucleic acid sequencing, amino acid sequencing, high performance liquid chromatography, capillary gel electrophoresis, near infrared spectroscopy, mass spectrometry, surface plasmon resonance technology, immuno-PCR technology, and biotin-avidin technology.

[0105] Preferably, the immunohistochemistry method comprises at least one of enzyme-linked immunosorbent assay, immunofluorescence assay, radioimmunoassay, immunocoprecipitation, immunochemiluminescence, colloidal gold immunoassay, fluorescence immunochromatography, complement fixation analysis, flow cytometry fluorescence resolution, and single molecule detection technology; further comprises enzyme-linked immunosorbent assay; and further comprises double antibody sandwich method.

[0106] Preferably, the substance for detecting the marker combination is selected from one or more of: a substance specific to the marker, a marker-specific probe, a gene chip, a protein chip, and a PCR primer.

[0107] Preferably, the substance specific for the marker comprises any one of h1) to h4):

[0108] h1) an antibody that specifically binds to the marker;

[0109] h2) a ligand protein or polypeptide that specifically binds to the marker;

[0110] h3) non-protein compounds that specifically recognize markers;

[0111] h4) Nucleic acid aptamers that specifically recognize markers.

[0112] Preferably, the antibody comprises at least one of a polyclonal antibody, a monoclonal antibody, a single-chain antibody, a functional antibody fragment, an antibody Fab region, a nanobody, a chimeric antibody, and a multispecific antibody.

[0113] Preferably, the product comprises at least one of a reagent, a kit, a test paper, a chip, a device, and a system.

[0114] Preferably, the product also contains substances for detecting other markers used for screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas, for example, the markers may be TOP1, C19ORF43, etc. disclosed in CN111455053A.

[0115] Preferably, the product further comprises auxiliary detection reagents for gene expression levels, and the auxiliary detection reagents for gene expression levels include: a reaction reagent for visualizing the amplicon corresponding to the primer, an RNA extraction reagent, a reverse transcription reagent, a cDNA amplification reagent, a standard substance for preparing a standard curve, and at least one of a positive control substance.

[0116] Preferably, the product further comprises an auxiliary detection reagent for protein expression, and the auxiliary detection reagent for protein expression comprises at least one of a color developer, a blocking solution, an antibody diluent, a washing buffer, a color stop solution, a standard substance for preparing a standard curve, and a positive control substance.

[0117] Preferably, the colorectal adenoma comprises colorectal advanced adenoma and / or colorectal non-advanced adenoma; more preferably, it is colorectal advanced adenoma.

[0118] Preferably, the diagnosis is to distinguish between a subject suffering from colorectal adenoma and a healthy subject.

[0119] The fourth aspect of the present invention is to provide a method for constructing a model, which comprises the step of constructing a model using the markers in the first aspect of the present invention.

[0120] Preferably, the method comprises the step of model building using the marker combination according to the second aspect of the present invention.

[0121] Preferably, the model is used for screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenoma.

[0122] Preferably, the algorithm for constructing the model includes at least one of logarithmic regression, linear discriminant analysis, eigengene linear discriminant analysis, support vector machine, random forest (RandomRorest), recursive partitioning tree, XGBoost, ShrunkenCentroids, StepAIC, Kth-Nearest Neighbor, Boosting, neural network, Bayesian network, and hidden Markov model; further includes at least one of random forest (RandomRorest) and XGBoost.

[0123] Preferably, the colorectal adenoma comprises colorectal advanced adenoma and / or colorectal non-advanced adenoma; more preferably, it is colorectal advanced adenoma.

[0124] Preferably, the diagnosis is to distinguish between a subject suffering from colorectal adenoma and a healthy subject.

[0125] A fifth aspect of the present invention is to provide a device or system comprising:

[0126] Detection module: used to detect the content, activity and / or expression of the marker in the first aspect of the present invention in the test sample; and

[0127] Analysis module: used to input the data acquired by the detection module into the model constructed according to the construction method of the fourth aspect of the present invention for analysis.

[0128] Preferably, the device or system is used for screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas.

[0129] Preferably, the detection module is used to detect the content, activity and / or expression level of the marker combination according to the second aspect of the present invention in a test sample.

[0130] Preferably, the test sample is the test sample in the first aspect of the present invention.

[0131] Preferably, the device or system further comprises: an output module for outputting information according to the analysis results obtained by the analysis module.

[0132] Preferably, the colorectal adenoma comprises colorectal advanced adenoma and / or colorectal non-advanced adenoma; more preferably, it is colorectal advanced adenoma.

[0133] Preferably, the diagnosis is to distinguish between a subject suffering from colorectal adenoma and a healthy subject.

[0134] A sixth aspect of the present invention provides a computer device, comprising a memory and a processor, wherein the memory stores a program, and when the processor executes the program, it implements any one of the following methods i1)-i2):

[0135] i1) The method for constructing the model according to the fourth aspect of the present invention;

[0136] i2)

[0137] S1: obtaining the content, activity and / or expression of the marker in the first aspect of the present invention in the test sample; and

[0138] S2: Input the data acquired in S1 into the model constructed according to the construction method of the fourth aspect of the present invention for analysis.

[0139] Preferably, S1 in i2) is to obtain the content, activity and / or expression level of the marker combination in the second aspect of the present invention in the test sample.

[0140] Preferably, the test sample is the test sample in the first aspect of the present invention.

[0141] Preferably, i2) further includes the following step: outputting information based on the obtained analysis results.

[0142] The seventh aspect of the present invention provides a computer-storable medium, wherein the computer-readable storage medium includes a stored computer program; wherein, when the computer program is running, the computer-readable storage medium is controlled to implement any one of methods i1)-i2) in the sixth aspect of the present invention.

[0143] The eighth aspect of the present invention provides a method for constructing a colorectal adenoma model, comprising adjusting the content, expression and / or activity of the markers of the first aspect of the present invention in the model:

[0144] When the content, expression level and / or activity of the marker in the test subject with colorectal adenoma is increased relative to that in the test subject without colorectal adenoma (preferably a healthy test subject), increasing the content, expression level and / or activity of the marker in the model;

[0145] When the content, expression level and / or activity of the marker in the subject suffering from colorectal adenoma is reduced relative to the subject not suffering from colorectal adenoma (preferably a healthy subject), the content, expression level and / or activity of the marker in the model is reduced.

[0146] Preferably, the model is an animal model.

[0147] Preferably, the animal is a rat, a mouse, or a guinea pig.

[0148] Preferably, the colorectal adenoma comprises colorectal advanced adenoma and / or colorectal non-advanced adenoma; more preferably, it is colorectal advanced adenoma.

[0149] The ninth aspect of the present invention provides a method for screening, diagnosing, monitoring, evaluating efficacy, or evaluating prognosis of colorectal adenomas, comprising the step of detecting the content, expression level and / or activity of the marker in the first aspect of the present invention in a test sample.

[0150] Preferably, the method comprises the step of detecting the content, expression level and / or activity of the marker combination in the second aspect of the present invention in the test sample.

[0151] Preferably, the test sample is the test sample in the first aspect of the present invention.

[0152] Preferably, the product of the first aspect of the present invention is used to detect the content, expression and / or activity of the marker of the first aspect of the present invention in a test sample.

[0153] Preferably, the product of the third aspect of the present invention is used to detect the content, expression level and / or activity of the marker combination of the second aspect of the present invention in a test sample.

[0154] Preferably, the method further comprises the step of inputting the data on the content, expression level and / or activity of the marker in the first aspect of the present invention or the combination of markers in the second aspect of the present invention in the obtained test sample into the model constructed according to the construction method of the fourth aspect of the present invention for analysis.

[0155] Preferably, the method further comprises the step of: outputting information according to the obtained analysis results.

[0156] Preferably, the colorectal adenoma comprises colorectal advanced adenoma and / or colorectal non-advanced adenoma; further, it is colorectal advanced adenoma.

[0157] Preferably, the diagnosis is to distinguish between a subject suffering from colorectal adenoma and a healthy subject.

[0158] The beneficial effects of the present invention are:

[0159] The present invention discloses for the first time ADGRE1, ADGRE5, AFM, APCS, BLNK, CABP2, CASP7, CCL25, CLIC5, COMP, ENPP2, F12, FASLG, FCER2, FCRL1, GAMT, GIP, HS3ST3B1, IGFBP1, IL4R, INPP1, ITPA, KAZN, KLK3, LPL, LSM1, LTA4H, MRPS16, NFASC, NOTCH3, NTF 3. One or more of PAIP2B, PPY, PRAME, SERPING1, SOD3, SOST, SPINT3, STX7, SULT2A1, SWAP70, TEX101, TMPRSS15, YJU2, and ZCCHC8 are used as markers for screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas. Screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas can be achieved by detecting the content, expression level and / or activity of the above markers. BRIEF DESCRIPTION OF THE DRAWINGS

[0160] Figure 1 shows the AUC comparison results of some single-marker RandomForest models and XGBboost models.

[0161] FIG2 is a ROC curve diagram of some 2-marker combinations (IL4R and PPY; FCRL1 and GIP).

[0162] Figure 3 is a graph showing the AUC comparison between the RandomForest model and the XGBboost model for some 2-markers combinations.

[0163] Figure 4 is the ROC curve diagram of some three-marker combinations (FCRL1, GIP and ZCCHC8; IL4R, INPP1 and PPY; IL4R, SULT2A1 and TMPRSS15), where A represents FCRL1, GIP and ZCCHC8; IL4R, INPP1 and PPY, and B represents IL4R, SULT2A1 and TMPRSS15.

[0164] Figure 5 is a graph showing the AUC comparison between the RandomForest model and the XGBboost model for some 3-markers combinations.

[0165] FIG6 is a ROC curve diagram of a partial 4-marker combination (IL4R, PPY, SULT2A1 and NFASC; GIP, F12, HS3ST3B1 and NTF3).

[0166] Figure 7 is a graph showing the AUC comparison between the RandomForest model and the XGBboost model for some 4-markers combinations.

[0167] FIG8 is a ROC curve diagram of a partial 5-marker combination (IL4R, PPY, SULT2A1, F12 and NTF3; GIP, F12, HS3ST3B1, ADGRE1 and NTF3).

[0168] Figure 9 is a graph showing the AUC comparison between the RandomForest model and the XGBboost model for some 5-markers combinations.

[0169] Figure 10 is a ROC curve diagram of some 6-markers combinations (IL4R, PPY, SULT2A1, COMP, SERPING1 and APCS; GIP, F12, HS3ST3B1, NTF3, ADGRE1 and SWAP70).

[0170] Figure 11 is a graph showing the AUC comparison between the RandomForest model and the XGBboost model for some 6-marker combinations.

[0171] Figure 12 is a ROC curve diagram of a partial 7-marker combination (IL4R, PPY, SULT2A1, STX7, NTF3, SERPING1 and ADGRE5; GIP, F12, HS3ST3B1, NTF3, ADGRE1, APCS and KAZN).

[0172] FIG13 is a graph showing the AUC comparison between the RandomForest model and the XGBboost model for some 7 marker combinations.

[0173] Figure 14 is a ROC curve diagram of some 8 marker combinations (IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, ADGRE5 and LSM1; GIP, F12, HS3ST3B1, NTF3, ADGRE1, LTA4H, KAZN and LSM1; PPY, CCL25, STX7, NFASC, IL4R, ENPP2, KAZN and LSM1), where A represents IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, ADGRE5 and LSM1; B represents GIP, F12, HS3ST3B1, NTF3, ADGRE1, LTA4H, KAZN and LSM1; PPY, CCL25, STX7, NFASC, IL4R, ENPP2, KAZN and LSM1.

[0174] FIG15 is a graph showing the AUC comparison between the RandomForest model and the XGBboost model for some 8-marker combinations. DETAILED DESCRIPTION

[0175] The present invention is further described in detail below through specific examples.

[0176] It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0177] The experimental methods in the following examples, where specific conditions are not specified, were generally performed under conventional conditions or the conditions recommended by the manufacturers. The materials and reagents used in these examples were commercially available unless otherwise specified.

[0178] The information of the proteins involved in the following examples is shown in Table 1.

[0179] Table 1 Protein information

[0180] Note: Ensembl ID is the gene search ID included in the Ensembl database; Uniprot ID is the protein search ID included in the Swiss-Prot sub-library of UniProt protein database.

[0181] Example 1 Diagnostic markers for colorectal adenoma

[0182] 1. Clinical Sample Collection

[0183] This example collected 39 patients diagnosed with colorectal advanced adenoma in the hospital and 40 healthy people as controls. Fresh blood samples were collected before surgery in the hospital, and the protein expression levels in the blood were detected by the protein detection olink platform as markers for modeling and prediction. According to the clinical progression of the patient's disease, the patient group was divided into two groups: 39 cases in the colorectal advanced adenoma group and 40 cases in the healthy person group. All patients with colorectal advanced adenoma enrolled in the group had new diseases and had no other serious diseases or cancers. They had not received surgery or other treatments at the time of sampling. The age range of the colorectal advanced adenoma group in the experimental group was 21-84 years old, with an average age of 55.2 years old and a male-to-female ratio of 28:11; the age range of the control group (healthy people) was 22-77 years old, with an average age of 51.5 and a male-to-female ratio of 9:31.

[0184] Sample inclusion criteria:

[0185] Inclusion criteria for the experimental group: (1) patients with confirmed preoperative advanced colorectal adenoma; (2) aged 18-80 years, regardless of gender; (3) no history of malignant tumors in other parts of the body; (4) no previous treatment with any other form of anti-tumor therapy before resection; (5) no history of blood transfusion in the past three years.

[0186] Sample exclusion criteria: (1) patients diagnosed with colorectal cancer who have completed surgical treatment or received radiotherapy, chemotherapy, and targeted drug treatment; (2) patients with other non-colorectal cancer tumors, or patients with colorectal metastasis from other tumors.

[0187] Clinical manifestations: 1) Experimental group: patients diagnosed with adenomas by colonoscopy who met one or more of the following criteria: a) diameter >10 mm; b) villous components; c) severe dysplasia or high-grade intraepithelial neoplasia. Some patients experienced bloody stools, colorectal cramping, anemia, weakness, and fatigue. 2) Control group: healthy subjects without symptoms such as intestinal discomfort.

[0188] In this example, the experimental and control groups were patients who visited the Sixth Affiliated Hospital of Sun Yat-sen University between April and August 2023 and were recruited in strict accordance with the above inclusion criteria. A total of 39 patients diagnosed with advanced colorectal adenomas and 40 healthy individuals were recruited. All specimen collections were approved by the Institutional Review Board of the Sixth Affiliated Hospital of Sun Yat-sen University with signed informed consent from the subjects' families.

[0189] 2. Blood Sample Processing

[0190] 5 mL of peripheral venous blood was collected from the patients and healthy controls using EDTA anticoagulant purple-top tubes, centrifuged for 10 minutes, and the upper plasma was collected and stored vertically in a -80°C refrigerator.

[0191] 3. Protein Detection

[0192] In this example, the inventors performed plasma proteomic testing on 79 plasma samples using the proximity extension technology PEA (Proximity Extension Assay) platform. Specifically, in the test, 3072 protein-specific antibodies were individually labeled with unique PEA oligonucleotide probes. Each antibody was labeled with two independent and complementary sequences. These bound antibodies were mixed into eight independent 384-well plates, each of which included three protein replicates for inter-plate quality control. During the test, 79 samples were randomly distributed on three different 96-well plates and incubated with antibodies labeled with PEA probes at +4°C overnight. After the immune reaction, the extension and pre-amplification mixture was added to the incubated samples for PCR amplification at room temperature. The PCR amplification products were then mixed before adding the individual sample index sequence, and then a second PCR amplification was performed. After sample mixing, the generated library was magnetic bead purified and quality controlled on a Bioanalyzer. Finally, sequencing was performed using an Illumina NovaSeq 6000 instrument, using two S1 flow cells with a read length of 2 × 50 bases. Counts of known barcode sequences were then converted to normalized protein expression units (NPX) through a quality control and normalization process, resulting in a total of 2,884 proteins being quantified.

[0193] 4. Construction of a colorectal adenoma protein marker screening model

[0194] The inventors used the XGBoost machine learning method to evaluate potential proteins that can distinguish colorectal adenomas from healthy people. To avoid bias in sample selection for machine learning modeling, 100 random modeling runs were performed using the Bootstrap method. In each modeling run, 70% (56 cases) of 39 samples of advanced colorectal adenomas and 40 healthy controls were selected as the training set (for screening single markers and the construction of marker combination models), and the remaining 30% (23 cases) were used as the test set (for validating the diagnostic efficacy of the markers and marker combinations screened from the training set).

[0195] (1) The independent predictive ability of each protein for colorectal adenoma was evaluated by AUC (area under the curve), sensitivity, and specificity. The mean and confidence interval were calculated 100 times. The predictive efficacy of the proteins was ranked, and proteins with an AUC greater than 0.7 were screened out.

[0196] (2) The XGBoost machine learning algorithm with no restriction on the number of proteins was used to build a model, and a model with high classification accuracy was screened out. The weight and frequency of each protein in the model were statistically analyzed, thereby identifying four key proteins, GIP, IL4R, PPY, and SPINT3, for use in predictive model construction.

[0197] (3) Among the 2884 proteins, the XGBoost machine learning method was used to evaluate the combination of each protein with one of GIP, IL4R, PPY, and SPINT3. AUC, sensitivity, and specificity were evaluated, and protein combinations with AUC greater than 0.80 were selected.

[0198] After the above three steps, the proteins obtained in each step were combined to obtain 45 markers: ADGRE1, ADGRE5, AFM, APCS, BLNK, CABP2, CASP7, CCL25, CLIC5, COMP, ENPP2, F12, FASLG, FCER2, FCRL1, GAMT, GIP, HS3ST3B1, IGFBP1, IL4R, INPP1, ITPA, KAZN, KLK3, LPL, LSM1, LTA4H, MRPS16, NFASC, NOTCH3, NTF3, PAIP2B, PPY, PRAME, SERPING1, SOD3, SOST, SPINT3, STX7, SULT2A1, SWAP70, TEX101, TMPRSS15, YJU2, and ZCCHC8; among them, 35 proteins showed significant differences, as shown in Table 2 (wherein, in Table 2: p is the t-test difference P value, p.adj is the t-test difference corrected P value, Mean_AP is the average expression value of the protein after standardization in colorectal adenoma, Mean_Healthy is the average expression value of the protein after standardization in healthy controls, and LogFC is the fold difference of the average expression values ​​between the two groups of samples (when LogFC is greater than 0, it indicates that the protein is highly expressed in colorectal adenoma patients; when LogFC is less than 0, it indicates that the protein is lowly expressed in colorectal adenoma patients), the same below); the prediction efficacy AUC values ​​of 45 proteins ranged from 0.54 to 0.7642, of which the prediction efficacy AUC values ​​of 35 proteins with significant differences ranged from 0.6188 to 0.7642; among them, the prediction efficacy AUC of 11 proteins was greater than 0.7, as shown in Table 3 (wherein, Mean_AUC in Table 3 represents the average AUC of 100 training classifications of the protein combination in the XGBoost machine learning model, sd_AUC is the standard deviation of the AUC of 100 training classifications, and Median_AUC is the median AUC of 100 training classifications, the same below). It can be seen that the above 45 markers can be used to diagnose colorectal adenomas.

[0199] Table 2 35 proteins with significant differences in the training set

[0200] Table 3: 11 proteins with prediction efficiency AUC greater than 0.7 in the test set

[0201] Furthermore, in order to verify the accuracy and stability of the above-selected markers, the inventors used the RandomForest machine learning method to perform modeling and prediction analysis on the 10 markers with better performance selected above. The modeling method is as follows: 70% (56 cases) of 39 colorectal adenoma samples and 40 healthy controls were randomly selected as training sets, and the remaining 30% (23 cases) were used as test sets. The model was trained 100 times, and then the average AUC of the 100 models was statistically compared with that of XGBboost. The results are shown in Figure 1: The AUC consistency of the RandomForest model and the XGBboost model is very good: the average AUC of the 100 random models is basically the same, and the standard deviation SD (black line) of the AUC of the 100 random models is also very close, that is, the stability is also very close; indicating the interchangeability of the model algorithm in the implementation scheme.

[0202] Among the 45 protein markers mentioned above, the combination marker examples are as follows. Combinations of, but not limited to, 2, 3, 4, 5, 6, 7, and 8 proteins are used, and the AUC, sensitivity, and specificity of each combination are evaluated using the XGBoost machine learning method. The Random Forest machine learning method is used to further verify the combinations with the best results, as follows:

[0203] Two-marker combinations are combinations of any two of the 45 markers mentioned above, totaling 990 combinations, such as IL4R and PPY; FCRL1 and GIP; and GIP and IL4R. The predictive efficacy of these 990 two-marker combinations ranged from 0.55 to 0.8582 (due to the large number of combinations, the AUC values ​​for each are not listed individually; only the AUC range for these 990 combinations is provided to demonstrate the diagnostic efficacy of these 990 two-marker combinations). Table 4 lists some two-marker combinations with AUCs greater than 0.80, with AUCs ranging from 0.8004 to 0.8582. The receiver operating characteristic (ROC) curves for some of these two-marker combinations (IL4R and PPY; FCRL1 and GIP) are shown in Figure 2. Consequently, these 990 two-marker combinations can be used to diagnose colorectal adenomas.

[0204] The AUC comparison results of the RandomForest model and the XGBboost model are shown in Figure 3. The AUC consistency of the RandomForest model and the XGBboost model is very good: the average AUC of the 100 random models is basically the same, and the standard deviation SD (black line) of the AUC of the 100 random models is also very close, that is, the stability is also very close, which shows the interchangeability of the model algorithms in the implementation scheme.

[0205] Table 4. Some 2-marker combinations with prediction effectiveness AUC greater than 0.80 in the test set

[0206] 3-marker combinations: any combination of three of the above 45 markers, totaling 14,190 combinations, such as FCRL1, GIP, and ZCCHC8; IL4R, INPP1, and PPY; IL4R, SULT2A1, and TMPRSS15; etc. The predictive efficacy AUC values ​​of these 14,190 3-marker combinations ranged from 0.5375 to 0.9161. (Due to the large number of combinations, the AUC values ​​of the 3-marker combinations are not listed one by one. Only the AUC value range of the 14,190 3-marker combinations is given to demonstrate the diagnostic efficacy of the above 14,190 3-marker combinations). Among them, some 3-marker combinations with AUC greater than or equal to 0.85 have a total of 14,190 combinations. The marker combinations are shown in Table 5 (where Mean_AUC represents the mean AUC of the protein combination across 100 training classifications in the XGBoost machine learning model, sd_AUC represents the standard deviation of the AUC across 100 training classifications, Median_AUC represents the median AUC across 100 training classifications, specificity represents the mean specificity across 100 training classifications, sensitivity represents the mean sensitivity across 100 training classifications, accuracy represents the mean accuracy across 100 training classifications, PPV represents the positive predictive value, and NPV represents the negative predictive value; the same applies below). AUC values ​​ranged from 0.85 to 0.9161, sensitivity values ​​ranged from 0.6041 to 0.9423, and specificity values ​​ranged from 0.6044 to 0.9446. Receiver operating characteristic (ROC) curves for some three marker combinations (FCRL1, GIP, and ZCCHC8; IL4R, INPP1, and PPY; and IL4R, SULT2A1, and TMPRSS15) are shown in Figures 4A and 4B. It can be seen that the above 14190 3-marker combinations can be used to diagnose colorectal adenomas.

[0207] The AUC comparison results of the RandomForest model and the XGBboost model are shown in Figure 5. The AUC consistency of the RandomForest model and the XGBboost model is very good: the average AUC of the 100 random models is basically the same, and the standard deviation SD (black line) of the AUC of the 100 random models is also very close, that is, the stability is also very close, which shows the interchangeability of the model algorithms in the implementation scheme.

[0208] Table 5: Some 3-marker combinations with predictive efficacy AUC greater than or equal to 0.85 in the test set

[0209] 4-marker combinations: any combination of four of the above 45 markers, totaling 148,995 combinations, such as IL4R, PPY, SULT2A1, and NFASC; GIP, F12, HS3ST3B1, and NTF3; GIP, TMPRSS15, CCL25, and APCS; etc. The predictive efficacy AUC values ​​of these 148,995 4-marker combinations range from 0.6865 to 0.9425. (Due to the large number of combinations, the AUC values ​​of the 4-marker combinations are not listed one by one. Only the AUC value range of the 148,995 4-marker combinations is given to demonstrate the diagnostic efficacy of the above 148,995 4-marker combinations). Among them, some 4-marker combinations with AUC greater than or equal to 0.90 have a total of 4 marker combinations with AUC greater than or equal to 0.90. The marker combinations are shown in Table 6. The AUC values ​​ranged from 0.900 to 0.9425, the sensitivity ranged from 0.6683 to 0.9372, and the specificity ranged from 0.6854 to 0.9516. The ROC curves for some four-marker combinations (IL4R, PPY, SULT2A1, and NFASC; GIP, F12, HS3ST3B1, and NTF3) are shown in Figure 6. As can be seen, the 148,995 four-marker combinations described above can be used to diagnose colorectal adenomas.

[0210] The AUC comparison results of the RandomForest model and the XGBboost model are shown in Figure 7. The AUC consistency of the RandomForest model and the XGBboost model is very good: the average AUC of the 100 random models is basically the same, and the standard deviation SD (black line) of the AUC of the 100 random models is also very close, that is, the stability is also very close, which shows the interchangeability of the model algorithms in the implementation scheme.

[0211] Table 6 Four marker combinations with prediction efficacy AUC greater than or equal to 0.90 in the test set

[0212] 5-marker combinations: Among the combinations with a 4-marker AUC greater than or equal to 0.9, and with three duplicates, the combination with the largest AUC was retained and the other combinations were eliminated, resulting in a total of 185 combinations retained (the 4-marker combinations marked in italics in Table 6 are any of the 185 4-marker combinations in the Summary of the Invention). The remaining 41 markers (i.e., 45 proteins minus the 4 proteins in the 4-marker combination) consist of 185×(45-4)=7585 5-marker combinations, such as IL4R, PPY, SULT2A1, F12, and NTF3; IL4R, PPY, SPINT3, COMP, and CLIC5; PPY, ZCCHC8, SPINT3, CCL25, and NFASC; etc. The predictive efficacy AUC values ​​of these 7585 5-marker combinations range from 0.8465 to 0.965. Due to the large number of combinations, the AUC values ​​of the 5-marker combinations are not listed one by one. Only the range values ​​of the AUC values ​​of the 7585 5-marker combinations are given to demonstrate the diagnostic effect of the above 7585 5-marker combinations); among them, some 5-marker combinations with AUC greater than or equal to 0.94 have a 5-marker combination with an AUC greater than or equal to 0.94. The marker combinations are shown in Table 7. Their AUC values ​​ranged from 0.94 to 0.965, their sensitivities from 0.7564 to 0.9464, and their specificities from 0.7556 to 0.9575. Some five-marker combinations with AUCs greater than 0.90 and less than 0.94, which were used to form six-marker combinations from the remaining 40 markers (i.e., 45 proteins minus the five proteins in the five-marker combination), are shown in Table 8. The receiver operating characteristic (ROC) curves for some of the five-marker combinations (IL4R, PPY, SULT2A1, F12, and NTF3; GIP, F12, HS3ST3B1, ADGRE1, and NTF3) are shown in Figure 8. Consequently, the 7585 five-marker combinations described above can be used to diagnose colorectal adenomas.

[0213] The AUC comparison results of the RandomForest model and the XGBboost model are shown in Figure 9. The AUC consistency of the RandomForest model and the XGBboost model is very good: the average AUC of the 100 random models is basically the same, and the standard deviation SD (black line) of the AUC of the 100 random models is also very close, that is, the stability is also very close, which shows the interchangeability of the model algorithms in the implementation scheme.

[0214] Table 7: Some 5-marker combinations with prediction effectiveness AUC greater than or equal to 0.94 in the test set

[0215] Table 8: Some of the 5 marker combinations with AUC greater than 0.90 and less than 0.94 used to form the 6 marker combination with the remaining 40 markers (i.e., 45 proteins minus the 5 proteins in the 5 marker combination)

[0216] 6-marker combinations: Among the combinations with a 5-marker AUC greater than 0.9, among the combinations with 4 duplicates, the combination with the largest AUC was retained and the other combinations were eliminated, resulting in a total of 143 combinations retained (the 5-marker combinations marked in italics in Table 7 and the 5-marker combinations in Table 8, i.e., the 143 5-marker combinations in the Summary of the Invention). The remaining 40 markers (i.e., 45 proteins minus the 5 proteins in the 5-marker combination) consist of 143×(45-5)=5720 6-marker combinations, such as IL4R, PPY, SULT2A1, COMP, SERPING1, and APCS; PPY, F12, HS3ST3B1, NTF3, SERPING1, and SWAP70; IL4R, PPY, SPINT3, COMP, STX7, and LTA4H; etc. The predictive efficacy AUC values ​​of these 5720 6-marker combinations range from 0.8915 to 0.9633. Due to the large number of combinations, the AUC values ​​of the 6-marker combinations are not listed one by one. Only the range values ​​of the AUC values ​​of the 5720 6-marker combinations are given to demonstrate the diagnostic effect of the above 5720 6-marker combinations); among them, some 6 markers with AUC greater than or equal to 0.95 have a total of 10 markers. The marker combinations are shown in Table 9. AUC values ​​ranged from 0.95 to 0.9633, sensitivities from 0.7830 to 0.9474, and specificities from 0.7864 to 0.9406. Receiver operating characteristic (ROC) curves for some six-marker combinations (IL4R, PPY, SULT2A1, COMP, SERPING1, and APCS; GIP, F12, HS3ST3B1, NTF3, ADGRE1, and SWAP70) are shown in Figure 10. Consequently, the 5720 six-marker combinations described above can be used to diagnose colorectal adenomas.

[0217] The AUC comparison results of the RandomForest model and the XGBboost model are shown in Figure 11. The AUC consistency of the RandomForest model and the XGBboost model is very good: the average AUC of the 100 random models is basically the same, and the standard deviation SD (black line) of the AUC of the 100 random models is also very close, that is, the stability is also very close, which shows the interchangeability of the model algorithms in the implementation scheme.

[0218] Table 9 Six marker combinations with prediction efficacy AUC greater than or equal to 0.95 in the test set

[0219] 7-marker combinations: Among the combinations with 6-marker AUC greater than or equal to 0.95, and with 5 repeated combinations, the combination with the largest AUC was retained and the other combinations were eliminated, resulting in a total of 163 combinations retained (the 6-marker combinations marked in italics in Table 9 and the 163 6-marker combinations in the Summary of the Invention). The remaining 39 markers (i.e., 45 proteins minus the 6 proteins in the 6-marker combination) comprise 163×(45-6)=6357 7-marker combinations, such as IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, and ADGRE5; PPY, SULT2A1, F12, NTF3, IL4R, SWAP70, and LTA4H; IL4R, PPY, LTA4H, STX7, SULT2A1, SERPING1, and HS3ST3B1; etc.; the predictive efficacy AUC values ​​of these 6357 7-marker combinations range from 0.8943 to 0.9733. (Due to the large number of combinations, the AUC values ​​of the 7-marker combinations are not listed one by one. Only the range of the AUC values ​​of the 6357 7-marker combinations is given to demonstrate the diagnostic efficacy of the above 6357 7-marker combinations); among them, some 7-marker combinations with AUC greater than or equal to 0.96 have a maximum predictive efficacy of 0.96. The marker combinations are shown in Table 10. AUC values ​​ranged from 0.96 to 0.9733, sensitivities from 0.8246 to 0.9568, and specificities from 0.8110 to 0.9245. The 7-marker combinations with AUCs greater than 0.95 and less than 0.96, which were used to form the 8-marker combinations from the remaining 38 markers (i.e., 45 proteins minus the 7 proteins in the 7-marker combination), are shown in Table 11. The receiver operating characteristic (ROC) curves for some of the 7-marker combinations (IL4R, PPY, SULT2A1, STX7, NTF3, SERPING1, and ADGRE5; GIP, F12, HS3ST3B1, NTF3, ADGRE1, APCS, and KAZN) are shown in Figure 12. As can be seen, the 6357 7-marker combinations described above can be used to diagnose colorectal adenomas.

[0220] The AUC comparison results of the RandomForest model and the XGBboost model are shown in Figure 13. The AUC consistency of the RandomForest model and the XGBboost model is very good: the average AUC of the 100 random models is basically the same, and the standard deviation SD (black line) of the AUC of the 100 random models is also very close, that is, the stability is also very close, which shows the interchangeability of the model algorithms in the implementation scheme.

[0221] Table 10: Some 7 marker combinations with prediction efficacy AUC greater than or equal to 0.96 in the test set

[0222] Table 11: Some 7 marker combinations with AUC greater than 0.95 and less than 0.96 for use with the remaining 38 markers (i.e., 45 proteins minus the 7 proteins in the 7 marker combination) to form the 8 marker combination

[0223] 8-marker combinations: Among the combinations with 7-marker AUC greater than 0.95 and with 6 duplicates, the combination with the largest AUC was retained and the other combinations were eliminated, resulting in a total of 137 combinations retained (the 7-marker combinations marked in italics in Table 10 and the 7-marker combinations in Table 11). The remaining 38 markers (i.e., 45 proteins minus the 7 proteins in the 7-marker combination) consist of 137×(45-7)=5206 8-marker combinations, such as IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, ADGRE5, and LSM1; GIP, F12, HS3ST3B1, NTF3, ADGRE1, LTA4H, KAZN, and LSM1; PPY, CCL25, STX7, NFASC, IL4R, ENPP2, KAZN, and LSM1; the predictive efficacy AUC values ​​of these 5206 8-marker combinations ranged from 0.9064 to 0.9762. (Due to the large number of combinations, the AUC values ​​of the 8-marker combinations are not listed one by one. Only the range of the AUC values ​​of the 5206 8-marker combinations is given to illustrate the above 5206 8-marker combinations.) The diagnostic efficacy of the 8-marker combination was evaluated. Table 12 shows some 8-marker combinations with an AUC greater than or equal to 0.95. The AUC values ​​ranged from 0.95 to 0.9762, the sensitivity ranged from 0.7878 to 0.9397, and the specificity ranged from 0.7830 to 0.9510. The receiver operating characteristic (ROC) curves for some 8-marker combinations (IL4R, PPY, SULT2A1, NTF3, STX7, SERPING1, ADGRE5, and LSM1; GIP, F12, HS3ST3B1, NTF3, ADGRE1, LTA4H, KAZN, and LSM1; and PPY, CCL25, STX7, NFASC, IL4R, ENPP2, KAZN, and LSM1) are shown in Figures 14A and 14B. Consequently, the 5206 8-marker combinations described above can be used to diagnose colorectal adenomas.

[0224] The AUC comparison results of the RandomForest model and the XGBboost model are shown in Figure 15. The AUC consistency of the RandomForest model and the XGBboost model is very good: the average AUC of the 100 random models is basically the same, and the standard deviation SD (black line) of the AUC of the 100 random models is also very close, that is, the stability is also very close, which shows the interchangeability of the model algorithms in the implementation scheme.

[0225] Table 12: Some 8-marker combinations with prediction effectiveness AUC greater than or equal to 0.95 in the test set

[0226] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be considered as equivalent replacement methods and are included in the scope of protection of the present invention.

Claims

1. Application of detection marker substances in product preparation: The product is used for: screening, diagnosis, monitoring, efficacy evaluation, or prognosis evaluation of colorectal adenomas; The marker is A, and A comprises at least one of B; The B is: TMPRSS15, ADGRE1, ADGRE5, AFM, APCS, BLNK, CABP2, CASP7, CCL25, CLIC5, COMP, ENPP2, F12, FASLG, FCER2, FCRL1, GAMT, GIP, HS3ST3B1, IGFBP1, IL4R, INPP1, I TPA, KAZN, KLK3, LPL, LSM1, LTA4H, MRPS16, NFASC, NOTCH3, NTF3, PAIP2B, PPY, PRAME, SERPING1, SOD3, SOST, SPINT3, STX7, SULT2A1, SWAP70, TEX101, YJU2, ZCCHC8.

2. The use according to claim 1, characterized in that: Said A comprises any 1, 2, 3, 4, 5, 6, 7 or 8 of said B; Preferably, said A comprises a single marker, and said single marker is any one of said B; Preferably, the single marker is: TMPRSS15, SPINT3, TEX101, IGFBP1, STX7, APCS, KLK3, IL4R, SULT2A1, SERPING1, CLIC5, F12, LTA4H, SOST, CCL25, HS3ST3B1, FASLG, PPY, LPL, GIP, ADGRE1, FCRL1, COMP, LSM1, ENPP2, FCER2, NOTCH3, ADGRE5, CABP2, GAMT, BLNK, NTF3, CASP7, NFASC, or ITPA.

3. The use according to claim 1, characterized in that: Said A comprises a combination of 2 markers, and said combination of 2 markers is any 2 of said B; Preferably, said A comprises a combination of 3 markers, and said combination of 3 markers is any 3 of said B; Preferably, said A comprises a combination of 4 markers, and said combination of 4 markers is any 4 of said B; Preferably, said A comprises a combination of 5 markers, and said combination of 5 markers is any five of said B; Preferably, the 5 markers are combined into a1) and a2): a1) the combination of the four markers; a2) any one of the above B; a2) is different from any one of the markers in the combination of 4 markers in a1); Preferably, said A comprises a combination of 6 markers, and said combination of 6 markers is any six of said B; Preferably, the 6 markers are combined into b1) and b2): b1) the 5 markers combination; b2) any one of the above B; b2) is different from any one of the markers in the combination of 5 markers in b1); Preferably, said A comprises a combination of 7 markers, and said combination of 7 markers is any seven of said B; Preferably, the 7 markers are combined into c1) and c2): c1) the 6 markers combination; c2) any one of the above B; Said c2) is different from any one of the markers in the combination of 6 markers in c1); Preferably, said A comprises an 8-markers combination, and said 8-markers combination is any eight of said B; Preferably, the 8 markers are combined into d1) and d2): d1) the 7 markers combination; d2) any one of the above B; The d2) is different from any one of the markers in the 7 markers combination in d1).

4. The use according to any one of claims 1 to 3, characterized in that: Said A further comprises at least one of G, the union of said G and said A in any one of claims 1 to 3 is said B, and The G has no intersection with the A in any one of claims 1 to 3; Preferably, the test sample of the product is selected from at least one of body fluids, tissues, cells, and excretions of the subject to be tested; Preferably, the body fluid comprises at least one of blood, lymph, pleural effusion, cerebrospinal fluid, joint fluid, ascites, saliva, lymph, and body fluid; Preferably, the tissue comprises intestinal mucosal tissue; Preferably, the blood comprises at least one of serum, plasma, dried blood spots, and whole blood; Preferably, the excreta comprises at least one of urine, feces, tears, and sweat; Preferably, the subject to be tested comprises a mammal; Preferably, the substance for detecting a marker comprises a substance for detecting a marker at the gene level and / or protein level; Preferably, the substance comprises a substance for one or more detection techniques or methods selected from the group consisting of immunohistochemistry, Western blotting, Northern blotting, polymerase chain reaction (PCR), biochip method, nucleic acid sequencing, amino acid sequencing, high performance liquid chromatography, capillary gel electrophoresis, near infrared spectroscopy, mass spectrometry, surface plasmon resonance technology, immuno-PCR technology, and biotin-avidin technology; Preferably, the immunohistochemistry method comprises at least one of enzyme-linked immunosorbent assay, immunofluorescence assay, radioimmunoassay, immunoprecipitation, immunochemiluminescence, colloidal gold immunoassay, fluorescence immunochromatography, complement fixation assay, flow cytometry fluorescence resolution, and single molecule detection technology; Preferably, the substance for detecting the marker is selected from: one or more of a substance specific to the marker, a marker-specific probe, a gene chip, a protein chip, and a PCR primer; Preferably, the substance specific for the marker comprises any one of h1) to h4): h1) an antibody that specifically binds to the marker; h2) a ligand protein or polypeptide that specifically binds to the marker; h3) non-protein compounds that specifically recognize markers; h4) nucleic acid aptamers that specifically recognize markers; Preferably, the antibody comprises at least one of a polyclonal antibody, a monoclonal antibody, a single-chain antibody, a functional antibody fragment, an antibody Fab region, a nanobody, a chimeric antibody, and a multispecific antibody; Preferably, the product comprises at least one of a reagent, a kit, a test paper, a chip, a device, and a system; Preferably, the product further comprises substances for detecting other markers used for screening, diagnosis, monitoring, efficacy evaluation, or prognostic assessment of colorectal adenomas; Preferably, the product further comprises auxiliary detection reagents for gene expression, and the auxiliary detection reagents for gene expression include: a reaction reagent for visualizing the amplicon corresponding to the primer, an RNA extraction reagent, a reverse transcription reagent, a cDNA amplification reagent, a standard substance for preparing a standard curve, and at least one of a positive control substance; Preferably, the product further comprises an auxiliary detection reagent for protein expression, and the auxiliary detection reagent for protein expression comprises at least one of a color developer, a blocking solution, an antibody diluent, a washing buffer, a color stop solution, a standard substance for preparing a standard curve, and a positive control substance. 5 . A marker combination comprising at least two of the Bs according to any one of claims 1 to 4 .

6. The marker combination according to claim 5, characterized in that: The marker combination comprises any 2, 3, 4, 5, 6, 7 or 8 of the B in any one of claims 1 to 4; Preferably, the marker combination comprises: e1)TMPRSS15; and e2) any one of the above B; Any one of the markers in e2) is different from that in e1); Preferably, the marker combination comprises: f1)IL4R; and f2) any one of the above B; Any one of the markers in f2) is different from f1); Preferably, the marker combination comprises: g1) TMPRSS15 and IL4R; Further preferably, the marker combination further comprises at least one of J, the union of J and g1) is B, and J has no intersection with g1); Preferably, the marker combination is A described in any one of claims 3-4.

7. A product comprising a substance for detecting the marker combination according to any one of claims 5-6.

8. The product according to claim 7, characterized in that: The substance for detecting the marker combination includes a substance for detecting the marker combination at the gene level and / or protein level; Preferably, the substance comprises a substance for one or more detection techniques or methods selected from the group consisting of immunohistochemistry, Western blotting, Northern blotting, PCR, biochip, nucleic acid sequencing, amino acid sequencing, high performance liquid chromatography, capillary gel electrophoresis, near infrared spectroscopy, mass spectrometry, surface plasmon resonance, immuno-PCR, and biotin-avidin technology; Preferably, the immunohistochemistry method comprises at least one of enzyme-linked immunosorbent assay, immunofluorescence assay, radioimmunoassay, immunoprecipitation, immunochemiluminescence, colloidal gold immunoassay, fluorescence immunochromatography, complement fixation assay, flow cytometry fluorescence resolution, and single molecule detection technology; Preferably, the substance for detecting the marker combination is selected from one or more of: a substance specific to the marker, a marker-specific probe, a gene chip, a protein chip, and a PCR primer; Preferably, the substance specific for the marker comprises any one of h1) to h4): h1) an antibody that specifically binds to the marker; h2) a ligand protein or polypeptide that specifically binds to the marker; h3) non-protein compounds that specifically recognize markers; h4) nucleic acid aptamers that specifically recognize markers; Preferably, the antibody comprises at least one of a polyclonal antibody, a monoclonal antibody, a single-chain antibody, a functional antibody fragment, an antibody Fab region, a nanobody, a chimeric antibody, and a multispecific antibody; Preferably, the product comprises at least one of a reagent, a kit, a test paper, a chip, a device, and a system; Preferably, the product further comprises substances for detecting other markers used for screening, diagnosis, monitoring, efficacy evaluation, or prognostic assessment of colorectal adenomas; Preferably, the product further comprises auxiliary detection reagents for gene expression, and the auxiliary detection reagents for gene expression include: a reaction reagent for visualizing the amplicon corresponding to the primer, an RNA extraction reagent, a reverse transcription reagent, a cDNA amplification reagent, a standard substance for preparing a standard curve, and at least one of a positive control substance; Preferably, the product further comprises an auxiliary detection reagent for protein expression, and the auxiliary detection reagent for protein expression comprises at least one of a color developer, a blocking solution, an antibody diluent, a washing buffer, a color stop solution, a standard substance for preparing a standard curve, and a positive control substance.

9. A method for constructing a model, comprising the step of constructing a model using the markers according to any one of claims 1 to 4.

10. The construction method according to claim 9, characterized in that: The method comprises the step of constructing a model using the marker combination according to claim 5 or 6; Preferably, the algorithm for constructing the model includes at least one of logarithmic regression, linear discriminant analysis, eigengene linear discriminant analysis, support vector machine, random forest, recursive partitioning tree, XGBoost, ShrunkenCentroids, StepAIC, Kth-NearestNeighbor, Boosting, neural network, Bayesian network, and hidden Markov model.

11. A device or system comprising: Detection module: used to detect the content, activity and / or expression of the marker described in any one of claims 1 to 4 in the test sample; and Analysis module: used to input the data obtained by the detection module into the model constructed according to the construction method according to claim 9 or 10 for analysis.

12. The device or system according to claim 11, characterized in that: The detection module is used to detect the content, activity and / or expression of the marker combination according to claim 5 or 6 in the test sample; Preferably, the test sample is the test sample described in claim 4; Preferably, the device or system further comprises: an output module for outputting information according to the analysis results obtained by the analysis module.

13. A computer device comprising a memory and a processor, wherein the memory stores a program, and when the processor executes the program, it implements any one of the following methods i1)-i2): i1) A method for constructing a model according to claim 9 or 10; i2) S1: Obtaining the content, activity and / or expression of the marker according to any one of claims 1 to 4 in the test sample; and S2: Input the data acquired in S1 into the model constructed according to the construction method according to claim 9 or 10 for analysis.

14. The computer device according to claim 13, wherein: i2) S1 is to obtain the content, activity and / or expression of the marker combination according to claim 5 or 6 in the test sample; Preferably, the test sample is the test sample described in claim 4; Preferably, i2) further includes the following step: outputting information based on the obtained analysis results.

15. A computer readable storage medium comprising a stored computer program; wherein: When the computer program is executed, the computer-readable storage medium is controlled to implement any one of the methods i1)-i2) in claim 13 or 14.

16. A method for screening, diagnosing, monitoring, evaluating therapeutic efficacy, or evaluating prognosis of colorectal adenoma, comprising the step of detecting the content, expression level and / or activity of the marker described in any one of claims 1 to 4 in a test sample.

Images