Reaction cup carrying device, photo-induced chemiluminescence detection system, and detection method

Abstract

A reaction cup carrying device, a photo-induced chemiluminescence detection system, and a detection method. The reaction cup carrying device comprises: a supporting mechanism (1) having an accommodation cavity (111); an incubation mechanism which comprises an incubation disc (21) rotatably provided in the accommodation cavity (111), wherein the incubation disc (21) can carry a plurality of reaction cups (100) in the circumferential direction thereof; and a photometric disc (71) which is rotatably provided in the accommodation cavity (111), wherein the photometric disc (71) can carry a plurality of reaction cups (100) in the circumferential direction thereof, the supporting mechanism (1) is provided with an excitation position and a detection position, and the excitation position and the detection position are located on the same circular path; one of the incubation disc (21) and the photometric disc (71) has an annular structure, and the other is arranged in the inner ring of the annular structure; the photometric disc (71) and the incubation disc (21) rotate independently around the same central axis. When samples have been incubated in the incubation disc, the reaction cups can be placed in the photometric disc by moving the reaction cups by a small distance to the inside or outside of the annular structure, thereby improving the detection efficiency and making the reaction cup carrying device have a rational and compact structure.

Description

Reaction cup support device, photo-induced chemiluminescence detection system and detection method

[0001] Citation of relevant applications

[0002] This application claims priority to Chinese patent application CN202410495277X, filed on April 23, 2024, the contents of which are incorporated herein by reference in their entirety and for all purposes. Technical Field

[0003] This application relates to the field of chemiluminescence immunoassay technology, and in particular to reaction cup support devices, photo-induced chemiluminescence detection systems, and detection methods. Background Technology

[0004] Chemiluminescence immunoassay (CLIA) is a technique that combines highly sensitive chemiluminescence assay with highly specific immunoreaction to detect and analyze various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins, and drugs.

[0005] Chemiluminescence immunoassay requires the assistance of a photo-induced chemiluminescence detection system. This system includes an optical detection mechanism for collecting and detecting light signals and an incubation mechanism for providing a constant temperature environment for the sample. After incubation in the incubation mechanism, the sample is transferred to the optical detection mechanism. However, the long distance between the incubation mechanism and the detection mechanism leads to low detection efficiency, and the photo-induced chemiluminescence detection system is not ideal and occupies a large space.

[0006] In addition, optical testing institutions may experience deviations in photometric values ​​after long-term use, necessitating the replacement of photometric components to ensure the accuracy of test results.

[0007] Furthermore, during optical inspection, the sample cannot be fixed and may shift during the inspection process, which can lead to deviations in the inspection results of the optical inspection agency.

[0008] Summary of the Invention

[0009] One object of this application is to provide a reaction cup support device to at least solve one of the above-mentioned technical problems.

[0010] To achieve the above objectives, the first aspect of this application provides a reaction cup support device, comprising:

[0011] Support mechanism with accommodating cavity;

[0012] Incubation mechanism, including an incubation tray rotatably disposed in the receiving cavity, the incubation tray being capable of holding multiple reaction cups along its circumference; and

[0013] The measuring optical disc is rotatably disposed in the receiving cavity. The measuring optical disc can carry multiple reaction cups along its circumference. The support mechanism is provided with an excitation position for receiving excitation light on the mixture in the reaction cup of the measuring optical disc and a detection position for detecting the mixture. The excitation position and the detection position are located on the same circumference.

[0014] One of the incubation disc and the measuring disc is a ring structure, and the other of the incubation disc and the measuring disc is located in the inner ring of the ring structure. The measuring disc and the incubation disc rotate independently around the same central axis.

[0015] Optionally, the reaction cup carrying device further includes a positioning mechanism disposed in the receiving cavity and used to keep the reaction cup on the measuring optical disc in the excitation position and / or detection position.

[0016] Optionally, the positioning mechanism includes a fixed block and a movable block. The fixed block is connected to the support mechanism, and the movable block is movably connected to the support mechanism to move closer to or further away from the fixed block. The reaction cup can move in and out of the fixed block and be sandwiched between the movable block and the fixed block.

[0017] Optionally, the positioning mechanism further includes a limiting block connected to the support mechanism. The limiting block has a guide groove, and the movable block is slidably disposed in the guide groove to move closer to or further away from the fixed block. The movable block and / or the fixed block have arc-shaped grooves for positioning the reaction cup.

[0018] Optionally, the positioning mechanism is an active support mechanism, including a driving component and a block connected to the driving component. The positioning mechanism is electrically connected to the obstacle sensor, and the block is moved by the driving component to achieve the positioning of the reaction cup.

[0019] Optionally, the incubation mechanism further includes an incubation reset detection unit, which is connected to the support mechanism and used to detect whether the incubation tray is at the zero position; and / or the incubation mechanism further includes an incubation step counting unit, which is connected to the support mechanism and used to detect the rotation angle of the incubation tray; and / or

[0020] The reaction cup support device further includes a photometric reset detection unit, which is connected to the support mechanism and is used to detect whether the photometric optical disc is at the zero position; and / or the reaction cup support device further includes a photometric step counting unit, which is connected to the support mechanism and is used to detect the rotation angle of the photometric optical disc.

[0021] Optionally, the reaction cup support device further includes a heating component and a temperature detection unit. The heating component is disposed on the support mechanism and is used to heat the sample in the receiving cavity. The temperature detection unit is used to detect the temperature in the receiving cavity.

[0022] Another objective of this application is to provide a photo-induced chemiluminescence detection system to at least solve one of the aforementioned technical problems.

[0023] To achieve this objective, the second aspect of this application adopts the following technical solution:

[0024] The photo-induced chemiluminescence detection system includes:

[0025] The aforementioned reaction cup support device; and

[0026] An optical detection mechanism is used to emit excitation light onto the mixture in the reaction vessel and to detect fluorescence in the mixture.

[0027] Optionally, the optical detection mechanism includes a photoexcitation component connected to the support mechanism, the photoexcitation component being used to emit excitation light onto the mixture within the reaction cup located at the excitation position.

[0028] Optionally, the optical detection mechanism further includes an optical detection component connected to the support mechanism. The optical detection component is used to detect fluorescence in the mixture in the reaction cup located at the detection position. The photoexcitation component and the optical detection component are arranged at intervals and located on the same circumferential trajectory.

[0029] Optionally, the optical inspection mechanism further includes a calibration module connected to the support mechanism, the calibration module being used to emit light of a preset intensity to the optical inspection mechanism to calibrate the optical inspection mechanism.

[0030] Another object of this application is to provide a detection method that at least solves one of the above-mentioned technical problems.

[0031] To achieve this objective, the third aspect of this application adopts the following technical solution:

[0032] The detection method, performed by the photo-induced chemiluminescence detection system, includes:

[0033] The incubation tray rotates to the first preset position, and the measuring disc rotates to the second preset position;

[0034] Transfer the preset reaction cups on the incubation tray to the test disc. The preset reaction cups contain the incubated samples.

[0035] The optical disc rotates to the third preset position so that the optical detection mechanism can detect the mixture in the preset reaction cup.

[0036] As can be seen from the above, the technical solution provided in this application, because one of the incubation tray and the measuring disc is a ring structure, and the other is located in the inner ring of the ring structure, allows the operator or automated transfer equipment to place the reaction cup in the measuring disc by simply gripping the reaction cup in the incubation tray and moving it a small distance inward or outward from the ring structure after the sample has been incubated in the incubation tray. This improves detection efficiency and makes the reaction cup carrying device structurally reasonable and compact. Furthermore, since the reaction cup on the measuring disc can rotate to the excitation and detection positions, rapid detection is achieved.

[0037] The photoexcitation component and the optical detection component are arranged at intervals and located on the same circumferential trajectory, so that the mixture can be irradiated by the excitation light in sequence and the fluorescence generated therefrom can be detected during the rotation of the measuring optical disc, thereby improving the detection efficiency.

[0038] The reaction cup carrier also includes a positioning mechanism, which is disposed in the receiving cavity and is used to keep the reaction cup on the measuring optical disc in the excitation position and / or detection position, so that the excitation light stably irradiates the mixture in the reaction cup, or the fluorescence in the reaction cup can be stably collected, thereby ensuring the accuracy of the detection results.

[0039] The optical inspection mechanism also includes a calibration module connected to the support mechanism. The calibration module is used to emit light of a preset intensity to the optical inspection mechanism to calibrate it, thereby avoiding deviations in photometric values ​​after long-term use of the optical inspection components and ensuring the accuracy of the inspection results. Attached Figure Description

[0040] Figure 1 is a partial cross-sectional view of the photo-induced chemiluminescence detection system provided in an embodiment of this application;

[0041] Figure 2 is a cross-sectional view of a partial reaction cup support device provided in an embodiment of this application;

[0042] Figure 3 is a schematic diagram of the positioning mechanism for fixing the reaction cup provided in an embodiment of this application;

[0043] Figure 4 is a schematic diagram of the photo-induced chemiluminescence detection system provided in an embodiment of this application;

[0044] Figure 5 is a cross-sectional view of the photo-induced chemiluminescence detection system provided in an embodiment of this application;

[0045] Figure 6 is a cross-sectional view of the photo-lasing component provided in an embodiment of this application;

[0046] Figure 7 is a cross-sectional view of the optical detection component provided in an embodiment of this application;

[0047] Figure 8 is a schematic diagram of the calibration module calibrating the optical detection component provided in an embodiment of this application;

[0048] Figure 9 is a cross-sectional view of the calibration module provided in an embodiment of this application.

[0049] In the diagram: 1. Support mechanism; 11. Receiving assembly; 111. Receiving cavity; 112. Disk body; 1121. Clearance hole; 113. Disk cover; 1131. Photometric cup gripping hole; 1132. Liquid injection hole; 1133. Incubation cup gripping hole; 1134. Cup adding hole; 1135. Excitation light hole; 1136. Photometric hole; 12. Support assembly; 121. Platform; 122. Support leg; 21. Incubation tray; 22. Incubation step counting unit; 23. Incubation reset detection unit; 24. Incubation code tooth disk; 241. Incubation code tooth; 25. Incubation drive assembly; 251. Incubation motor; 252. Incubation belt; 3. Photolysis assembly; 31. Photolysis sleeve; 311. Photolysis step hole; 32. Photolysis convex lens; 33. Lower photolysis pressure block; 34. 4. Optical Detection Module; 35. Upper Optical Excitation Block; 36. Optical Excitation Fixing Component; 4. Optical Detection Housing; 42. Filter; 43. Optical Plano-Convex Lens; 44. Photon Counter; 45. First Washer; 46. Filter Press; 47. Metering Press; 48. Metering Fixing Block; 5. Calibration Module; 51. Calibration Housing; 511. Cavity; 512. Top Wall; 513. Connecting Hole; 52. Attenuation Press; 53. Calibration Light Source; 54. Attenuator; 55. Calibration Board; 56. Second Washer; 6. Heating Module; 61. Heating Element; 62. Electrical Connector; 63. Fixing Plate; 71. Metering Optical Disc; 72. Metering Step Counting Unit; 73. Metering Reset Detection Unit; 74. Metering Code Gear Disc; 741. Photometric code teeth; 75. Photometric drive assembly; 751. Photometric motor; 752. Photometric belt; 8. Temperature detection unit; 9. Positioning mechanism; 91. Fixed block; 911. Arc groove; 92. Movable block; 93. Limiting block; 931. Guide groove; 94. Elastic element; 100. Reaction cup. Detailed Implementation

[0050] The technical solution of this application will be further described below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely for explaining this application and not for limiting it. Furthermore, it should be noted that, for ease of description, only the parts relevant to this application are shown in the accompanying drawings, not all of them.

[0051] This application defines certain directional terms. Unless otherwise stated, the directional terms used, such as "up," "down," "left," "right," "inner," and "outer," are used for ease of understanding and therefore do not constitute a limitation on the scope of protection of this application.

[0052] In this application, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Moreover, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0053] In the description of this application, unless otherwise expressly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0054] This embodiment provides a reaction cup support device, which can be used in photo-induced chemiluminescence detection, but is not limited thereto. As shown in Figures 1-5, the reaction cup support device provided in this embodiment includes a support mechanism 1, an incubation mechanism, and a photometric auxiliary mechanism. The support mechanism 1 is used to support the incubation mechanism and the photometric auxiliary mechanism.

[0055] The support mechanism 1 has a receiving cavity 111 to accommodate at least part of the incubation mechanism and at least part of the photometric auxiliary mechanism.

[0056] The incubation mechanism includes an incubation tray 21 rotatably disposed in the receiving cavity 111, the incubation tray 21 being capable of supporting a plurality of reaction cups 100 along its circumference. For example, the incubation tray 21 has a circular structure, and a plurality of first insertion holes are spaced apart on the same circumference of the incubation tray 21, the reaction cups 100 being inserted into the first insertion holes and thus disposed on the incubation tray 21.

[0057] To increase the capacity of the incubation tray 21, optionally, multiple rings of first insertion holes can be formed along the radial direction of the incubation tray 21, as shown in Figure 1. In this embodiment, two rings of first insertion holes are formed on the incubation tray 21. It is understood that in other embodiments, the incubation tray 21 may have only one ring of first insertion holes, or more than two rings of first insertion holes. The incubation tray 21 is not limited to a circular ring, but may also be a square ring, a polygonal ring, or other annular structures.

[0058] The first insertion hole extends along the axial direction of the incubation tray 21, and the outer extension of the reaction cup 100 is supported on the surface of the incubation tray 21. The distance between the outer wall of the reaction cup 100 in the first insertion hole and the hole wall of the first insertion hole is no more than 1 / 3 of the wall thickness of the reaction cup 100. This allows for rapid and uniform heating of the mixture in the reaction cup 100, while also facilitating the removal and placement of the reaction cup 100.

[0059] The photometric auxiliary mechanism includes a photometric optical disc 71, which is rotatably disposed in the receiving cavity 111. The photometric optical disc 71 can support multiple reaction cups 100 along its circumference. Exemplarily, the photometric optical disc 71 has a ring structure or a disc structure. Multiple second insertion holes are spaced apart on the same circumference of the photometric optical disc 71, and the reaction cups 100 can be inserted into the second insertion holes and thus disposed on the photometric optical disc 71. In this embodiment, a ring of second insertion holes is formed along the radial direction of the photometric optical disc 71.

[0060] The second socket extends along the axial direction of the measuring optical disc 71, and the outer extension of the reaction cup 100 is supported on the surface of the measuring optical disc 71. The distance between the outer wall of the reaction cup 100 in the second socket and the hole wall of the second socket is no more than 1 / 3 of the wall thickness of the reaction cup 100. This allows for rapid and uniform heating of the mixture in the reaction cup 100, while also facilitating the removal and placement of the reaction cup 100.

[0061] The measuring optical disc 71 is disposed in the inner ring of the annular structure, and the measuring optical disc 71 and the incubation disc 21 rotate independently around the same central axis. It is understood that in other optional embodiments, the measuring optical disc 71 may also be an annular structure, and the incubation disc 21 may be located in the inner ring of the annular structure.

[0062] Optionally, the incubation disc 21 and the light measuring disc 71 are rotatably connected to the same fixed axis (not shown in the figure). For example, an incubation sleeve (not shown in the figure) and a light measuring sleeve (not shown in the figure) are connected to the fixed axis. The incubation sleeve is rotatably connected to the fixed axis through a bearing (not shown in the figure), and the light measuring sleeve is rotatably connected to the fixed axis through another bearing (not shown in the figure), so that the incubation disc 21 and the light measuring disc 71 can rotate independently around the same central axis.

[0063] The support mechanism 1 is equipped with an excitation position and a detection position. The reaction cup 100 on the measuring optical disc 71 can rotate to the excitation position and the detection position. When the reaction cup 100 rotates to the excitation position, the mixture inside the reaction cup 100 can receive the excitation light; when the reaction cup 100 on the measuring optical disc 71 rotates to the detection position, the mixture inside the reaction cup 100 is detected. The excitation position and the detection position are on the same circumference.

[0064] For example, the circumference diameter of the excitation position and the detection position is the same as the circumference diameter of the second socket on the measuring disc 71, so that during the rotation of the measuring disc 71, the multiple reaction cups 100 on the measuring disc 71 can pass through the excitation position and the detection position in sequence, thereby enabling the mixture in the reaction cups 100 to be detected one by one and improving the detection efficiency.

[0065] Because one of the incubation tray 21 and the measuring disc 71 has a ring structure, and the other is located within the inner ring of the ring structure, after the sample (containing the reaction cup 100) has been incubated in the incubation tray 21, the operator or automated transfer equipment can easily place the reaction cup 100 into the measuring disc 71 by moving it a small distance inward or outward from the ring structure. This improves detection efficiency and makes the reaction cup support device structurally reasonable and compact. Furthermore, since the reaction cup 100 on the measuring disc 71 can rotate to the excitation and detection positions, rapid detection is achieved.

[0066] As shown in Figure 1, optionally, the support mechanism 1 may include a receiving component 11 and a support component 12. A receiving cavity 111 (as shown in Figure 5) is formed in the receiving component 11. The support component 12 is connected to the receiving component 11 and can support the receiving component 11.

[0067] Optionally, the receiving assembly 11 may include a disc body 112 and a disc cover 113. The disc body 112 has a receiving groove, and the disc cover 113 covers the opening of the receiving groove to form a receiving cavity 111. The disc body 112 and the disc cover 113 can prevent or reduce the light entering the receiving cavity 111, thereby preventing the light from affecting the detection results. For example, the disc body 112 and the disc cover 113 can be connected by bolts or other fasteners, or by snap-fit ​​or other means. This structure of the receiving assembly 11 facilitates the placement of the incubation disc 21 and the measuring disc 71 in the receiving groove. It is understood that during the detection process, the incubation disc 21 and the measuring disc 71 will rotate relative to the support mechanism 1, while the support mechanism 1 remains stationary, that is, the disc body 112 and the disc cover 113 remain stationary.

[0068] The support assembly 12 may include a tabletop 121 and legs 122. The disc 112 housing the assembly 11 is connected to the tabletop 121 by bolts or the like. The legs 122 are connected to the underside of the tabletop 121 to support it. There may be multiple legs 122, which are spaced apart circumferentially along the tabletop 121 to stably support it.

[0069] The support mechanism 1 also has a cup gripping position, and the reaction cups 100 on the measuring disc 71 and the incubation disc 21 can be rotated to the cup gripping position so that the reaction cups 100 are transferred from the incubation disc 21 to the measuring disc 71 at the cup gripping position.

[0070] Specifically, the cover 113 has an incubation cup gripping hole 1133 and a photometric cup gripping hole 1131 at the position corresponding to the cup gripping position. After the sample in the reaction cup 100 on the incubation tray 21 has completed incubation, the incubation tray 21 rotates, causing the reaction cup 100 to rotate below the incubation cup gripping position, that is, below the incubation cup gripping hole 1133. The operator or automated device grips the reaction cup 100 through the incubation cup gripping hole 1133. At the same time or before this, the second insertion hole on the photometric disc 71 that does not carry the reaction cup 100 rotates to the lower side of the photometric cup gripping hole 1131, so that the reaction cup 100 enters the second insertion hole through the photometric cup gripping hole 1131.

[0071] The cover 113 can also have an injection hole 1132. Tools such as injection guns can inject general liquid or other reagents into the reaction cup 100 located on the measuring disc 71 through the injection hole 1132, so that the reagents can be mixed with the sample in the reaction cup 100 to form a mixture.

[0072] An excitation aperture 1135 and a metering aperture 1136 can also be provided on the cover 113. The excitation aperture 1135 is located at the excitation position, and the metering aperture 1136 is located at the detection position.

[0073] After the mixture (containing the reaction cup 100) is detected at the detection position, it continues to rotate with the measuring optical disc 71. When the mixture (containing the reaction cup 100) that has completed the detection moves to the photometric cup grasping hole 1131 again, the operator or the automated device can also grasp the reaction cup 100 on the measuring optical disc 71 through the photometric cup grasping hole 1131 and then discard the reaction cup 100.

[0074] Optionally, the excitation aperture 1135, the metering aperture 1136, the liquid injection aperture 1132, and the metering cup aperture 1131 are on the same circumference, and the diameter of this circumference is the same as the diameter of the circumference containing the second insertion hole. This ensures that during the rotation of the measuring disc 71, each second insertion hole is directly opposite the excitation aperture 1135, the metering aperture 1136, the liquid injection aperture 1132, and the metering cup aperture 1131, respectively. Furthermore, when the measuring disc 71 stops rotating, the excitation aperture 1135, the metering aperture 1136, the liquid injection aperture 1132, and the metering cup aperture 1131 can each be directly opposite a first insertion hole. This allows for simultaneous operation of multiple reaction cups, improving detection efficiency.

[0075] For example, the photometric cup aperture 1131, the liquid injection aperture 1132, the excitation aperture 1135, and the photometric aperture 1136 are arranged sequentially along the rotation direction of the photometric disc 71. In this way, for the same reaction cup 100, when the photometric disc 71 rotates, the reaction cup 100 is aligned with the photometric cup aperture 1131, the liquid injection aperture 1132, the excitation aperture 1135, and the photometric aperture 1136 in sequence, and finally returns to the photometric cup aperture 1131. Thus, during the rotation of the photometric disc 71, the reaction cup 100 and the sample or mixture inside the reaction cup 100 are operated sequentially, ultimately realizing photo-induced chemiluminescence detection.

[0076] Optionally, a cup-adding hole 1134 may be provided on the cover 113, through which an operator or automated device adds the reaction cup 100 into the first insertion hole of the incubation tray 21. For example, the cup-adding hole 1134 and the incubation cup-grabbing hole 1133 are located on the same circumference, and the diameter of this circumference is the same as the diameter of the circumference containing the first insertion hole, so that multiple second insertion holes align with either the cup-adding hole 1134 or the incubation cup-grabbing hole 1133 during rotation. Furthermore, when the cup-adding hole 1134 aligns with a first insertion hole, the incubation cup-grabbing hole 1133 also aligns with a first insertion hole, allowing simultaneous operation on two reaction cups 100 and improving detection efficiency.

[0077] It is understood that each ring of the first insertion hole corresponds to a set of cup-adding holes 1134 and incubation cup-grabbing holes 1133. In this embodiment, there are two rings of the first insertion hole, so there are two sets of cup-adding holes 1134 and incubation cup-grabbing holes 1133.

[0078] As shown in Figures 2 and 3, the reaction cup support device further includes a positioning mechanism 9. The positioning mechanism 9 is disposed in the receiving cavity 111 and is used to maintain the reaction cup 100 on the measuring optical disc 71 at the excitation position and / or the detection position, so that the excitation light stably irradiates the mixture in the reaction cup 100, or the fluorescence in the reaction cup 100 can be stably collected, thereby ensuring the accuracy of the detection results. In this embodiment, positioning mechanisms 9 are provided at both the excitation position and the detection position, so that the reaction cup 100 is positioned at the excitation position and the detection position, respectively.

[0079] The positioning mechanism 9 may include a fixed block 91 and a movable block 92. The fixed block 91 is connected to the support mechanism 1, and the movable block 92 is movably connected to the support mechanism 1 to move closer to or further away from the fixed block 91. The reaction cup 100 can move in and out of the fixed block 91 and be sandwiched between the movable block 92, thereby ensuring that when the detection disk is not rotating, the reaction cup 100 is stably located in the excitation or detection position, and that when the detection disk rotates, the reaction cup 100 can continue to rotate with the detection disk. Specifically, both the fixed block 91 and the movable block 92 are connected to the disk cover 113.

[0080] In other alternative embodiments, the movable block 92 can also be an elastic block, such as an elastic block made of silicone, rubber or other materials. When the reaction cup 100 enters between the fixed block 91 and the movable block 92, the movable block 92 is squeezed and deformed by the reaction cup 100. The movable block 92 applies a reaction force to the reaction cup 100, so that the reaction cup 100 is stably located between the fixed block 91 and the movable block 92.

[0081] In other optional embodiments, the positioning mechanism can be an active support mechanism, meaning the positioning mechanism can actively move to position the reaction cup 100. The positioning mechanism includes a driving component and a block connected to the driving component. The driving component can be a cylinder, hydraulic cylinder, etc., and the block is a block connected to the driving component such as a cylinder or hydraulic cylinder. An obstacle sensor can detect whether the reaction cup 100 is in a predetermined position. The obstacle sensor can be electrically connected to the driving component. When it is detected that the reaction cup 100 has moved to the predetermined position as the measuring disc 71 rotates, the block is actively extended by the driving component to clamp the reaction cup 100 in the preset position, which is either an excitation position or a detection position. Furthermore, both the obstacle sensor and the driving component are electrically connected to a controller. The obstacle sensor transmits a signal to the controller, which controls the driving component to move, thereby moving the block. The controller can be a centralized or distributed controller. For example, the controller can be a single microcontroller or composed of multiple distributed microcontrollers. The microcontroller can run a control program to control the aforementioned components to achieve their functions.

[0082] To improve the stability of the movable block 92 during movement, the positioning mechanism 9 may optionally include a limiting block 93 connected to the support mechanism 1. The limiting block 93 has a guide groove 931, and the movable block 92 is slidably disposed in the guide groove 931. The guide groove 931 guides the movable block 92, allowing it to move closer to or away from the fixed block 91. Specifically, the guide groove 931 is a U-shaped groove with an opening facing the fixed block 91. The movable block 92 protrudes from the opening into the guide groove 931 to press against the side of the reaction cup 100.

[0083] Optionally, the movable block 92 and the limiting block 93 are connected by an elastic element 94. The elastic element 94 can drive the movable block 92 to move towards the side where the fixed block 91 is located, thereby clamping the reaction cup 100 between the movable block 92 and the fixed block 91. Optionally, the elastic element 94 is a spring, with one end connected to the bottom of the guide groove 931 and the other end connected to the movable block 92.

[0084] When the reaction cup 100 is not placed between the movable block 92 and the fixed block 91, the distance between the movable block 92 and the fixed block 91 is less than the diameter of the reaction cup 100. The detection plate drives the reaction cup 100 to rotate, and the reaction cup 100 gradually enters between the movable block 92 and the fixed block 91. The spring is compressed, and a force is applied to the movable block 92 through the spring. The reaction cup 100 is clamped between the fixed block 91 and the movable block 92.

[0085] When the reaction cup 100 is sandwiched between the fixed block 91 and the movable block 92, to improve the accuracy of the position of the reaction cup 100 and ensure the consistency of the relative positions of each reaction cup 100 with the movable block 92 and the fixed block 91, optionally, the movable block 92 and / or the fixed block 91 are provided with arc-shaped grooves 911 for positioning the reaction cup 100. The arc-shaped grooves 911 can position the reaction cup 100 so that the reaction cup 100 does not move after entering the arc-shaped grooves 911, and prevent the reaction cup 100 from sliding relative to the fixed block 91 or the movable block 92 due to compression after the detection plate stops rotating, which would cause the position of the reaction cup 100 to be inaccurate.

[0086] As shown in Figure 4, the incubation mechanism may further include an incubation reset detection unit 23, which is connected to the support mechanism 1 and is used to detect whether the incubation tray 21 is in the zero position. It is understood that the incubation tray 21 returns to the zero position every 360° rotation.

[0087] The incubation mechanism may also include an incubation step counting unit 22, which is connected to the support mechanism 1 and is used to detect the rotation angle of the incubation tray 21. The incubation reset detection unit 23 and the incubation step counting unit 22 can be photoelectric sensors, and can both be connected to the underside of the platform 121.

[0088] Specifically, the incubation mechanism may further include an incubation gear disk 24, which rotates synchronously with the incubation disk 21. For example, the incubation gear disk 24 is fixedly connected to the incubation bushing to rotate synchronously with the incubation disk 21. The incubation gear disk 24 may be located below the platform 121. The incubation gear disk 24 has an incubation reset baffle, and the incubation reset detection unit 23 detects whether the incubation disk 21 is at the zero position through the incubation reset baffle.

[0089] Specifically, when the incubation reset baffle is located between the receiving end and the transmitting end of the incubation reset detection unit 23, it indicates that the incubation disk 21 is reset, that is, the incubation disk 21 is in the zero position. For example, there is one incubation reset baffle, so that the incubation reset baffle enters and exits the incubation reset detection unit 23 once for every 360° rotation of the incubation disk 21.

[0090] The incubation code disk 24 may also have multiple incubation code teeth 241, which are evenly distributed along the circumference of the incubation code disk 24. The incubation step counting unit 22 detects the rotation angle of the incubation disk 21 through the incubation code teeth 241.

[0091] Specifically, when the incubation code tooth 241 can enter and exit between the receiving end and the transmitting end of the incubation reset detection unit 23, the number of rotation steps and rotation angle of the incubation disk 21 are obtained by detecting the number of times the incubation code tooth 241 enters and exits the incubation reset detection unit 23. Optionally, when the incubation code tooth disk 24 rotates, the incubation step counting unit 22 performs one count each time the incubation code tooth 241 enters between the receiving end and the transmitting end of the incubation reset detection unit 23.

[0092] Optionally, the number of incubation code teeth 241 is the same as the number of first sockets, and the incubation code teeth 241 and the first sockets are set in a one-to-one correspondence. By reasonably designing the relative position of the incubation code tooth disk 24 and the incubation disk 21, and reasonably designing the position of the photometer cup grasping hole 1131 and the cup adding hole 1134, when the incubation code teeth 241 enter the receiving end and the transmitting end of the incubation reset detection unit 23, the incubation cup grasping hole 1133 and the cup adding hole 1134 each correspond to a first socket. Then, the operator or the automated device performs corresponding operations on the positions of the incubation cup grasping hole 1133 and the cup adding hole 1134, such as grasping the reaction cup 100 corresponding to the incubation cup grasping hole 1133, or inserting the reaction cup 100 into the first socket through the cup adding hole 1134.

[0093] The photometering auxiliary mechanism may also include a photometering reset detection unit 73, which is connected to the support mechanism 1 and is used to detect whether the photometering disc 71 is in the zero position. It is understood that the photometering disc 71 returns to the zero position every 360° rotation.

[0094] The reaction cup support device also includes a photometric step counting unit 72, which is connected to the support mechanism 1 and is used to detect the rotation angle of the photometric optical disc 71. The photometric reset detection unit 73 and the photometric step counting unit 72 can be photoelectric sensors, and can both be connected to the underside of the platform 121.

[0095] Specifically, the photometering mechanism may also include a photometering code disk 74, which rotates synchronously with the photometering optical disk 71. For example, the photometering code disk 74 is fixedly connected to the photometering shaft sleeve to rotate synchronously with the photometering optical disk 71, and the photometering code disk 74 may be located below the table surface 121.

[0096] The photometer code disk 74 has a photometer reset baffle. The photometer reset detection unit 73 detects whether the photometer optical disk 71 is in the zero position through the photometer reset baffle. Specifically, when the photometer reset baffle is located between the receiving end and the transmitting end of the photometer reset detection unit 73, it indicates that the photometer optical disk 71 is reset, that is, the photometer optical disk 71 is in the zero position. For example, there is one photometer reset baffle, so that the photometer reset baffle enters and exits the incubation reset detection unit 23 once for every 360° rotation of the photometer optical disk 71.

[0097] The photometering code disk 74 may also have multiple photometering codes 741, which are evenly distributed along the circumference of the photometering code disk 74. The photometering step counting unit 72 detects the rotation angle and the number of rotation steps of the photometering disk 71 through the photometering codes 741. Specifically, when the photometering codes 741 can enter and exit between the receiving end and the transmitting end of the photoelectric sensor, the rotation angle of the photometering disk 71 is obtained by detecting the number of times the photometering codes 741 enter and exit the photoelectric sensor. Optionally, when the photometering code disk 74 rotates, the photometering step counting unit 72 performs one count each time the photometering codes 741 enters between the receiving end and the transmitting end of the photometering step counting unit 72.

[0098] Optionally, the number of photometric code teeth 741 is the same as the number of first sockets, and the photometric code teeth 741 and the first sockets are set in a one-to-one correspondence. By reasonably designing the relative positions of the photometric code tooth disk 74 and the photometric disk 71, and reasonably designing the positions of the excitation aperture 1135, the photometric aperture 1136, the liquid injection aperture 1132 and the photometric cup grasping aperture 1131, when the photometric code teeth 741 enter the receiving end and the transmitting end of the photometric step counting unit 72, the excitation aperture 1135, the photometric aperture 1136, the liquid injection aperture 1132 and the photometric cup grasping aperture 1131 each correspond to a second socket, thereby enabling the automated device or optical detection mechanism (described in detail below) to simultaneously operate the reaction cups 100 corresponding to the excitation aperture 1135, the photometric aperture 1136, the liquid injection aperture 1132 and the photometric cup grasping aperture 1131.

[0099] As shown in Figure 4, optionally, the incubation mechanism further includes an incubation drive assembly 25 connected to the support mechanism 1, which drives the incubation disk 21 to rotate. For example, the incubation drive assembly 25 can be connected to an incubation bushing so that by driving the incubation bushing to rotate, the incubation bushing drives the incubation gear disk 24 and the incubation disk 21 to rotate synchronously.

[0100] Specifically, the incubation drive assembly 25 includes an incubation motor 251, an incubation belt 252, an incubation drive pulley, and an incubation driven pulley. The incubation motor 251 is connected to the underside of the platform 121. The incubation drive pulley is connected to the output end of the incubation motor 251. The incubation driven pulley is fixed to the outside of the incubation bushing. The incubation belt 252 is sleeved on the incubation drive pulley and the incubation driven pulley. When the incubation motor 251 rotates, it drives the incubation drive pulley to rotate, which in turn drives the incubation belt 252 to rotate. The incubation belt 252 drives the incubation driven pulley to rotate. Since the incubation driven pulley is connected to the incubation bushing, the rotation of the incubation driven pulley drives the incubation bushing to rotate, which in turn drives the incubation gear disc 24 and the incubation disc 21 to rotate synchronously.

[0101] The metering auxiliary mechanism also includes a metering drive assembly 75 connected to the support mechanism 1. The metering drive assembly 75 is used to drive the metering optical disc 71 to rotate. For example, the metering drive assembly 75 can be connected to the metering bushing so that by driving the metering bushing to rotate, the metering gear disk 74 and the metering optical disc 71 can rotate synchronously. Specifically, the metering drive assembly 75 includes a metering motor 751, a metering belt 752, a metering drive pulley, and a metering driven pulley. The metering motor 751 is connected to the lower side of the platform 121, the metering drive pulley is connected to the output end of the metering motor 751, the metering driven pulley is fixed to the outside of the metering bushing, and the metering belt 752 is sleeved on the metering drive pulley and the metering driven pulley. The rotation of the photometer motor 751 can drive the photometer active pulley to rotate, which in turn drives the photometer belt 752 to rotate. The photometer belt 752 drives the photometer driven pulley to rotate. Since the photometer driven pulley is connected to the photometer bushing, the rotation of the photometer driven pulley drives the photometer bushing to rotate, which in turn drives the photometer code tooth disk 74 and the photometer optical disk 71 to rotate synchronously.

[0102] As shown in Figures 1 and 7, the reaction cup support device also includes a heating component 6 and a temperature detection unit 8. The heating component 6 is disposed on the support mechanism 1 and is used to heat the sample in the receiving cavity 111. The temperature detection unit 8 is used to detect the temperature in the receiving cavity 111.

[0103] Since the heating component 6 is mounted on the support mechanism 1, it will not rotate during the rotation of the incubation tray 21 and the measuring disc 71, thus facilitating the fixation of the heating component 6. In addition, during the rotation of the incubation tray 21, the reaction cup 100 located on the incubation tray 21 will generate turbulence in the air within the containment cavity 111, thereby further improving the temperature uniformity within the containment cavity 111.

[0104] Optionally, the heating assembly 6 includes a heating element 61 and an electrical connector 62. The electrical connector 62 is electrically connected to the heating element 61 and can be connected to a power source to supply power to the heating element 61, thereby causing the heating element 61 to heat up.

[0105] As shown in Figure 5, the heating element 61 is attached to the lower surface of the disk body 112. The heating element 61 is preferably located on the outside of the receiving cavity 111. The heat generated by the heating element 61 is conducted to the receiving cavity 111 through the disk body 112, thereby further improving the temperature uniformity within the disk body 112. Optionally, the heating assembly 6 may also include a fixing plate 63, which is disposed on the outer surface of the heating element 61 and connected to the disk body 112 by bolts or the like, thereby fixing the heating element 61.

[0106] As shown in Figure 1, a clearance hole 1121 may be provided on the side wall of the disk body 112, and the electrical connector 62 passes through the clearance hole 1121 and is then connected to the power supply.

[0107] Continuing with Figure 1, this embodiment also provides a photo-induced chemiluminescence detection system, which includes the aforementioned reaction cup support device and an optical detection mechanism. The optical detection mechanism is used to emit excitation light into the mixture within the reaction cup 100 and to detect fluorescence in the mixture. This embodiment integrates the optical detection mechanism and the reaction cup support device into a single structure, thereby further improving the compactness of the photo-induced chemiluminescence detection system.

[0108] Optionally, the optical detection mechanism includes a photoexcitation component 3 connected to the support mechanism 1. The photoexcitation component 3 is used to emit excitation light into the mixture within the reaction cup 100 located at the excitation position. In a specific embodiment, the photoexcitation component 3 is used to emit 680nm light, which can excite a specific substance in the mixture, causing the specific substance to fluoresce in a specific wavelength band.

[0109] In order to detect fluorescence, the optical detection mechanism also includes an optical detection component 4 connected to the support mechanism 1. The optical detection component 4 is used to detect the fluorescence in the mixture in the reaction cup 100 located at the detection position. The photoexcitation component 3 and the optical detection component 4 are arranged at intervals and located on the same circumferential trajectory, so that the mixture can be irradiated by the excitation light in sequence during the rotation of the measuring disc 71, and the fluorescence generated therefrom can be detected, thereby improving the detection efficiency.

[0110] In this embodiment, the photoexcitation assembly 3 and the optical detection assembly 4 are respectively connected to the disk cover 113. The photoexcitation assembly 3 is positioned opposite to the excitation aperture 1135 so that the excitation light emitted by the photoexcitation assembly 3 enters the mixture in the reaction cup 100 through the excitation aperture 1135. The optical detection assembly 4 is positioned opposite to the photometer aperture 1136 so that the optical detection assembly 4 collects the fluorescence generated by the mixture through the photometer aperture 1136. Since the disk cover 113 remains stationary during the detection process, the optical detection mechanism can also remain stationary, thereby ensuring the accuracy of the optical detection mechanism.

[0111] As shown in Figure 6, the photoexcitation assembly 3 includes a photoexcitation sleeve 31, a photoexcitation lens 32, a lower photoexcitation block 33, and a photoexciter 34. The photoexcitation sleeve 31 is connected to the cover 113 of the support mechanism 1. The photoexcitation sleeve 31 has a photoexcitation stepped hole 311, which is opposite to the excitation light hole 1135. The photoexcitation lens 32 is disposed in the photoexcitation stepped hole 311 and abuts against the stepped surface of the photoexcitation stepped hole 311, thereby supporting the photoexcitation lens 32 through the stepped surface.

[0112] The lower photoexcitation block 33 is disposed in the photoexcitation stepped hole 311 and presses against the photoexcitation convex lens 32 to fix the photoexcitation convex lens 32. That is, the photoexcitation convex lens 32 is fixed between the stepped surface of the photoexcitation stepped hole 311 and the lower photoexcitation block 33.

[0113] Optionally, the photo-convex lens 32 is a plano-convex lens, with the convex side of the plano-convex lens contacting the step surface to adapt to the step surface. The plane of the plano-convex lens is in contact with the lower photo-stimulating block 33, thereby allowing the lower photo-stimulating block 33 to stably press against the photo-convex lens 32.

[0114] The photoexciter 34 is disposed in the photoexcitation step hole 311 and is used to emit excitation light to the photoexcitation convex lens 32. Specifically, the photoexciter 34 can emit light at 680nm. The photoexciter 34 can abut against the upper end of the lower photoexcitation block 33, which is a hollow annular structure to allow light from the photoexciter 34 to pass through.

[0115] The photoexcitation assembly 3 may further include an upper photoexcitation block 35, which presses against the upper end of the photoexciter 34 to fix the photoexciter 34 between the upper photoexcitation block 35 and the lower photoexcitation block 33. Optionally, the upper photoexcitation block 35 and the photoexcitation sleeve 31 are connected by bolts or other connecting parts. Optionally, the upper photoexcitation block 35 has a hollow structure so that the wires of the photoexciter 34 pass through the upper photoexcitation block 35 to connect to the power supply, and so that the exciter 34 is accommodated within the hollow structure of the upper photoexcitation block 35.

[0116] The photo-lasing assembly 3 may also include a photo-lasing fixing member 36, which has a through hole opposite to the excitation light hole 1135. The photo-lasing sleeve 31 is disposed in the through hole. The photo-lasing fixing member 36 is connected to the receiving assembly 11. For example, the photo-lasing fixing member 36 is connected to the disk cover 113 by screws or the like, thereby fixing the photo-lasing assembly 3 to the disk cover 113.

[0117] As shown in Figure 7, the optical detection assembly 4 includes an optical detection housing 41 and a filter 42, an optical plano-convex mirror 43, and a photon counter 44, all disposed within the optical detection housing 41. The filter 42 is directly opposite the photometer aperture 1136. The photon counter 44 is used to detect the intensity of fluorescence. The filter 42 filters out other light in the mixture, allowing only the preset fluorescence to pass through. The optical plano-convex mirror 43 increases the width of the fluorescence light beam to improve the accuracy of the photon counter 44.

[0118] In this embodiment, the filter 42, the optical plano-convex mirror 43, and the photon counter 44 are arranged sequentially. Fluorescence in the detected mixture passes sequentially through the photometer aperture 1136, the filter 42, and the optical plano-convex mirror 43 into the photon counter 44. Alternatively, in other optional embodiments, the optical plano-convex mirror 43, the filter 42, and the photon counter 44 can also be arranged sequentially, with fluorescence passing sequentially through the photometer aperture 1136, the optical plano-convex mirror 43, and the filter 42 into the photon counter 44. In this embodiment, the filter 42 only allows fluorescence at 660nm to pass through. However, in other optional embodiments, the light allowed through the filter 42 is not limited to this.

[0119] The optical detection assembly 4 may also include a filter press 46, which is disposed on the side of the filter 42 away from the optical plano-convex mirror 43 to support the filter 42.

[0120] A through hole can be formed on the bottom wall of the optical detection housing 41. The through hole can be a stepped hole. The filter press 46, the filter 42 and the optical plano-convex mirror 43 are disposed in the through hole. The photon counter 44 is disposed inside the optical detection housing 41. Of course, in other optional embodiments, the filter 42 and the optical plano-convex mirror 43 can also be disposed inside the optical detection housing 41.

[0121] The optical detection assembly 4 may also include a photometering pressure block 47 and a photometering fixing block 48. The photometering pressure block 47 and the photometering fixing block 48 are disposed in the through hole of the bottom wall of the optical detection housing 41. The photometering pressure block 47 may be disposed above the photometering fixing block 48. The filter pressure plate 46, the filter 42 and the optical plano-convex lens 43 are sandwiched between the photometering pressure block 47 and the photometering fixing block 48.

[0122] Both the photometering pressure block 47 and the photometering fixing block 48 have stepped through holes to allow fluorescence to pass through the filter 42 and the optical plano-convex lens 43. Optionally, in this embodiment, the optical plano-convex lens 43 is disposed between the photon counter 44 and the filter 42, and the stepped surface of the photometering pressure block 47 abuts against the convex surface of the optical plano-convex lens 43 to limit the optical plano-convex lens 43. A first washer 45 is provided between the optical plano-convex lens 43 and the filter 42. The first washer 45 can be an O-ring washer, which allows for a gap between the filter 42 and the optical plano-convex lens 43, thereby adjusting the beam width of the fluorescence.

[0123] The filter press 46 is disposed on the lower side of the filter 42 and abuts against the stepped surface of the photometer fixing block 48, thereby fixing the optical plano-convex lens 43, the filter 42 and the filter press 46.

[0124] Optionally, the photometer fixing block 48 is provided with an external thread, and the through hole on the bottom wall of the optical detection housing 41 is provided with an internal thread. The photometer fixing block 48 is threadedly connected to the bottom wall, thereby fixing the photometer fixing block 48 to the optical detection housing 41, and fixing the filter 42 and the optical plano-convex lens 43.

[0125] As shown in Figure 5, the optical detection mechanism also includes a calibration module 5 connected to the support mechanism 1. The calibration module 5 is used to emit light of a preset intensity (hereinafter referred to as standard light) to the optical detection mechanism to calibrate the optical detection mechanism, thereby avoiding deviations in the photometric values ​​of the optical detection component 4 after long-term use and ensuring the accuracy of the detection results.

[0126] As shown in Figures 5 and 8, specifically, the optical detection mechanism is connected to the lower side of the stage 121 and located outside the receiving cavity 111. The optical detection mechanism and the optical detection component 4 are arranged facing each other in the vertical direction so that the standard light emitted by the calibration module 5 enters the optical detection component 4.

[0127] During the calibration process, the calibration module 5 emits standard light to the optical detection component 4 and adjusts the detection value of the optical detection component 4 so that the value is consistent with the value that should be detected (since the intensity of the standard light emitted by the calibration module 5 is a preset intensity, the value detected by the optical detection component 4 should also be a preset value), thus achieving the calibration of the optical detection component 4.

[0128] As shown in Figure 8, it is understandable that a reaction cup 100 can be placed between the optical detection component 4 and the calibration module 5 during calibration, but the reaction cup 100 needs to be unloaded to eliminate the influence of the reaction cup 100 on the detection results during the detection process. Of course, it is also understandable that the reaction cup 100 can be omitted between the optical detection component 4 and the calibration module 5, and the detection results can be corrected based on experience.

[0129] As shown in Figure 9, the calibration module 5 includes a calibration shell 51, a calibration light source 53, and an attenuator 54. The calibration shell 51 is connected to the support mechanism 1. The calibration light source 53 and the attenuator 54 are both disposed inside the calibration shell 51. The calibration light source 53 is used to generate light, and the attenuator 54 is used to reduce the intensity of the light. The support mechanism 1 has a calibration hole that is directly opposite to the attenuator 54. The light emitted by the calibration light source 53 can be received by the optical detection component 4 through the attenuator 54 and the calibration hole.

[0130] Optionally, the calibration housing 51 is connected to the lower side of the platform 121, and the calibration light source 53 is connected to the calibration board 55 to control the on / off state of the calibration light source 53. A cavity 511 is formed inside the calibration housing 51, and the calibration light source 53 is located at the bottom of the cavity 511. A stepped hole for fixing the attenuator 54 is formed on the top wall 512 of the calibration housing 51.

[0131] It is understandable that when calibrating the optical detection component 4, if the intensity of the standard light is not significantly different from the fluorescence intensity produced by the mixture, the accuracy of the calibration results can be improved. However, due to limitations in the amount of the mixture and the amount of the analyte in the mixture, the fluorescence intensity may be low. In this embodiment, the main function of the attenuator 54 is to reduce the intensity of light entering the optical detection component 4.

[0132] In addition, to further reduce the intensity of light entering the optical detection component 4, the calibration light source 53 and the attenuator 54 are offset in the vertical direction, thereby reducing the intensity of light from the calibration light source 53 entering the optical detection component 4.

[0133] In addition, the cavity 511 and the stepped hole on the top wall 512 of the calibration shell 51 are connected by a connecting hole 513. That is, a connecting hole 513 is also provided on the top wall 512 of the calibration shell 51. The diameter of the connecting hole 513 is smaller than the diameter of the stepped hole on the top wall 512 of the calibration shell 51, so as to further reduce the intensity of the light from the calibration light source 53 entering the optical detection component 4.

[0134] Optionally, a second washer 56 is provided at the bottom of the stepped hole on the top wall 512 of the calibration housing 51, and the attenuator 54 is disposed on the second washer 56. An attenuation block 52 is also provided on the upper side of the attenuator 54, thereby fixing the attenuator 54 in the stepped hole of the calibration housing 51. Optionally, the attenuation block 52 has a through hole to allow light to pass through. The outer periphery of the attenuation block 52 has an external thread, and the stepped hole on the top wall 512 of the calibration housing 51 has an internal thread, thereby allowing the attenuation block 52 and the calibration housing 51 to be connected by threads.

[0135] For example, the working process of the photo-induced chemiluminescence detection system provided in this embodiment is as follows:

[0136] S1. Heating component 6 heats the sample to bring the temperature inside the cavity 111 to the temperature required for incubating the sample.

[0137] S2. The incubation drive assembly 25 drives the incubation disk 21 and the incubation code disk 24 to rotate. When the incubation code teeth 241 on the incubation code disk 24 enter the incubation step counting unit 22, the first socket with no surface load is aligned with the cup-adding hole 1134. The reaction cup 100 containing the sample is inserted into the first socket by the operator or automated device. At the same time, the reaction cup 100 in one of the first sockets is aligned with the incubation cup-grabbing hole 1133. When aligned with the incubation cup-grabbing hole 1133... When the sample incubation in the reaction cup 100 is completed, the reaction cup 100 is removed by an operator or an automated device. Specifically, by reasonably designing the rotation speed of the incubation disc 21, the number of rotations of the incubation disc 21, and / or the spacing between the incubation cup gripping hole 1133 and the cup adding hole 1134, the sample incubation is completed when the reaction cup 100 is inserted into the first insertion hole through the incubation cup gripping hole 1133 and the reaction cup 100 rotates with the incubation disc 21 to the incubation cup gripping hole 1133.

[0138] S3. Step S3 can be performed synchronously with step S2. The photometric drive component 75 drives the photometric optical disc 71 and the photometric code tooth disc 74 to rotate, so that the unloaded second socket is aligned with the photometric cup grasping hole 1131 or the detected reaction cup 100 is aligned with the photometric cup grasping hole 1131.

[0139] S4. In step S2, the operator or automated device will take the reaction cup 100 taken from the incubation tray 21 and insert it into the second socket through the photometric cup grasping hole 1131 (it can be understood that if there is a reaction cup 100 in the second socket directly opposite the photometric cup grasping hole 1131, the reaction cup 100 will be taken out first).

[0140] S5 can be performed simultaneously with step S4. The operator or automated device drips reagents such as general liquid into the reaction cup 100, which is directly opposite the general liquid hole, through the liquid injection hole 1132, so that the sample in the reaction cup 100 forms a mixture; the photoexcitation component 3 emits excitation light into the reaction cup 100, which is directly opposite the excitation light hole 1135; the optical detection component 4 detects the mixture in the reaction cup 100, which is directly opposite the light metering hole 1136, through the light metering hole 1136.

[0141] The photo-induced chemiluminescence detection system provided in this embodiment can operate on multiple reaction cups 100 simultaneously, which can improve detection efficiency.

[0142] This embodiment also discloses a detection method, wherein the above-described photo-induced chemiluminescence detection system performs the detection method, which includes:

[0143] The incubation tray 21 rotates to a first preset position, and the photometric optical disc 71 rotates to a second preset position. Specifically, the first preset position is when the reaction cup 100 to be transferred after incubation on the incubation tray 21 is located in the incubation claw cup position, that is, the reaction cup 100 to be transferred after incubation on the incubation tray 21 is directly opposite the incubation claw cup hole 1133. The second preset position is when the unused second socket on the photometric optical disc 71 rotates to the claw cup position, or the tested reaction cup 100 rotates to the claw cup position, that is, the unused second socket on the photometric optical disc 71 is directly opposite the photometric claw cup hole 1131, or the tested reaction cup 100 is directly opposite the photometric claw cup hole 1131.

[0144] The preset reaction cup 100 on the incubation tray 21 is transferred to the photometric tray 71. The preset reaction cup 100 contains the incubated sample. That is, the operator or the automated device picks up the reaction cup 100 facing the incubation cup picking hole 1133 and puts it into the second socket facing the photometric cup picking hole 1131. If there is a detected reaction cup 100 in the second socket facing the photometric cup picking hole 1131, the reaction cup 100 is taken out first.

[0145] The measuring optical disc 71 rotates to a third preset position so that the optical detection mechanism can detect the mixture in the preset reaction cup 100. Specifically, the third preset position is directly below the optical detection mechanism. Furthermore, there are two third preset positions: the first is directly below the photoexcitation component 3, and the second is directly below the optical detection component 4. The measuring optical disc 71 rotates so that the reaction cup 100 on the measuring optical disc 71 sequentially enters the positioning mechanism 9 located below the excitation aperture 1135 (at this time, the reaction cup 100 is located in the first third preset position) and the positioning mechanism 9 located below the photometer aperture 1136 (at this time, the reaction cup 100 is located in the second third preset position).

[0146] The detection method may further include exciting the mixture; for example, the photoexcitation component 3 emits excitation light into the mixture within the reaction vessel 100 to excite fluorescent molecules within the mixture. Furthermore, the photoexcitation component 3 emits excitation light into the reaction vessel 100 located at a first and third predetermined position.

[0147] The detection method may also include detecting a mixture; for example, the optical detection component 4 detects the fluorescence intensity of the mixture within the reaction vessel 100. Furthermore, the optical detection component 4 detects the fluorescence intensity within the reaction vessel 100 located at a second or third preset position.

[0148] Although this application has been described in detail above with general descriptions, specific embodiments, and experiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of this application fall within the scope of protection claimed in this application.

Claims

1. A reaction cup support device, characterized in that, include: The support mechanism (1) has a receiving cavity (111); The incubation mechanism includes an incubation tray (21) rotatably disposed in the receiving cavity (111), the incubation tray (21) being capable of holding a plurality of reaction cups (100) along its circumference; and The measuring optical disc (71) is rotatably disposed in the receiving cavity (111). The measuring optical disc (71) can carry multiple reaction cups (100) along its circumference. The support mechanism (1) is provided with an excitation position for receiving excitation light in the reaction cups (100) of the measuring optical disc (71) and a detection position for detecting the mixture. The excitation position and the detection position are located on the same circumference. One of the incubation disc (21) and the measuring disc (71) is a ring structure, and the other of the incubation disc (21) and the measuring disc (71) is located in the inner ring of the ring structure. The measuring disc (71) and the incubation disc (21) rotate independently around the same central axis.

2. The reaction cup support device according to claim 1, characterized in that, The reaction cup support device further includes a positioning mechanism (9), which is disposed in the receiving cavity (111) and is used to keep the reaction cup (100) on the measuring optical disc (71) in the excitation position and / or detection position.

3. The reaction cup support device according to claim 2, characterized in that, The positioning mechanism (9) includes a fixed block (91) and a movable block (92). The fixed block (91) is connected to the support mechanism (1), and the movable block (92) is movably connected to the support mechanism (1) to move closer to or further away from the fixed block (91). The reaction cup (100) can move in and out of the fixed block (91) and be sandwiched between the movable block (92).

4. The reaction cup support device according to claim 2, characterized in that, The positioning mechanism is an active support mechanism, including a driving component and a block connected to the driving component. The positioning mechanism is electrically connected to an obstacle sensor, and the block is moved by the driving component to achieve the positioning of the reaction cup (100).

5. The reaction cup support device according to any one of claims 1-4, characterized in that, The incubation mechanism further includes an incubation reset detection unit (23), which is connected to the support mechanism (1) and is used to detect whether the incubation tray (21) is at the zero position; and / or the incubation mechanism further includes an incubation step counting unit (22), which is connected to the support mechanism (1) and is used to detect the rotation angle of the incubation tray (21); and / or The reaction cup support device further includes a photometric reset detection unit (73), which is connected to the support mechanism (1) and is used to detect whether the photometric optical disc (71) is at the zero position; and / or the reaction cup support device further includes a photometric step counting unit (72), which is connected to the support mechanism (1) and is used to detect the rotation angle of the photometric optical disc (71).

6. The reaction cup support device according to any one of claims 1-4, characterized in that, The incubation tray (21) has a first insertion hole extending axially along the incubation tray (21). The outer extension of the reaction cup (100) is supported on the surface of the incubation tray (21). The distance between the outer wall of the reaction cup (100) in the first insertion hole and the hole wall of the first insertion hole is not greater than 1 / 3 of the wall thickness of the reaction cup (100); and / or The measuring optical disc (71) is provided with a second insertion hole, which extends along the axial direction of the measuring optical disc (71). The outer extension of the reaction cup (100) is supported on the surface of the measuring optical disc (71). The distance between the outer wall of the reaction cup (100) in the second insertion hole and the hole wall of the second insertion hole is not greater than 1 / 3 of the wall thickness of the reaction cup (100).

7. A photo-induced chemiluminescence detection system, characterized in that, include: The reaction cup support device according to any one of claims 1-6; as well as An optical detection mechanism is used to emit excitation light into the mixture within the reaction vessel (100) and to detect fluorescence in the mixture.

8. The photo-induced chemiluminescence detection system according to claim 7, characterized in that, The optical detection mechanism includes a photoexcitation component (3) connected to the support mechanism (1), the photoexcitation component (3) being used to emit excitation light into the mixture in the reaction cup (100) located at the excitation position; The optical detection mechanism further includes an optical detection component (4) connected to the support mechanism (1). The optical detection component (4) is used to detect fluorescence in the mixture in the reaction cup (100) located at the detection position. The photoexcitation component (3) and the optical detection component (4) are arranged at intervals and located on the same circumferential trajectory.

9. The photo-induced chemiluminescence detection system according to claim 8, characterized in that, The optical detection mechanism also includes a calibration module (5) connected to the support mechanism (1). The calibration module (5) is used to emit light of a preset intensity to the optical detection mechanism to calibrate the optical detection mechanism.

10. A detection method, characterized in that, The photo-induced chemiluminescence detection system according to any one of claims 7-9 performs the detection method, the detection method comprising: The incubation tray (21) is rotated to the first preset position, and the measuring disc (71) is rotated to the second preset position; The preset reaction cup (100) on the incubation tray (21) is transferred to the test disc (71), and the preset reaction cup (100) contains the incubated sample; The optical disc (71) is rotated to the third preset position so that the optical detection mechanism can detect the mixture in the preset reaction cup (100).

Images