Composition based on bamboo extract, selenium and polyphenols for improving hair and nail health

Abstract

The present invention relates to a composition comprising, as an active ingredient, a mixture comprising a bamboo shoot extract, polyphenols and selenium, and also to the uses thereof, in particular as a food supplement, pharmaceutical product, cosmetic product or nutrition product. This specific combination of ingredients is of particular interest for improving the quality of the hair and nails, in particular for improving the growth thereof and the strengthening thereof.

Description

Formula based on bamboo extract, selenium and polyphenols to improve hair and nail health technical field

[0001] The invention relates to a composition comprising, as an active ingredient, a mixture of bamboo shoot extract, polyphenols, and selenium, and its uses, in particular, as a food supplement, pharmaceutical product, cosmetic product, or nutritional product. This specific combination of ingredients is of particular interest for improving the quality of hair and nails, especially for enhancing their growth and strengthening. State of the art

[0002] The human body is composed of epidermal appendages, or skin appendages, such as nails and the pilosebaceous unit; the latter comprising the hair shaft and the hair follicle. These appendages are primarily composed of keratin, which is a component of the skin and all skin appendages (hair, nails, etc.), thus giving hair and nails their structure and ensuring their strength, shine, and hydration by preventing water evaporation. Finally, it also protects them from external aggressions.

[0003] Hair growth occurs at the level of the hair follicle, located at the junction of the deep dermis and the hypodermis. Its nutrition is ensured by the blood supply via small vessels that reach the hair shaft, thus providing the amino acids, minerals, vitamins, and growth hormones that the hair needs to maintain its health. Furthermore, the hair is naturally lubricated by sebum, which is produced by the sebaceous glands that surround the hair shaft.

[0004] Certain alterations can appear with age or due to extrinsic factors (environmental, mechanical, chemical, nutritional, etc.) or intrinsic factors (hormonal, oxidative stress, health status, etc.). These alterations can cause a decrease in hair density, accelerating hair loss, or making hair drier and more brittle. Various signaling pathways strongly regulate hair growth, resulting from a balance between inhibitory factors, which slow hair growth, and stimulatory factors, which promote it. The hair cycle is thus composed of three phases alternating between growth, rest, and shedding cycles, namely: respectively the anagen, catagen and telogen phases.

[0005] The anagen phase represents the hair growth period. It constitutes the longest part of the hair cycle, lasting from 2 to 8 years. Consequently, the vast majority of hairs on our heads are in the anagen phase. During this phase, metabolic activity in the hair bulb is intense, resulting in a high production of keratin and melanin. The mitotic activity of the matrix cells is also very significant, driving the growth of the hair shaft. The longer the anagen phase, the more the hair can grow and the longer it becomes.

[0006] Conversely, if the start of the anagen phase is delayed, it leads to disrupted regeneration. This largely explains the difference between individuals, some of whom can have very long hair while others do not. The anagen phase is followed for 2 to 3 weeks by a catagen phase, which is a transitional phase between the growth and resting phases, during which the hair stops growing.

[0007] Finally, the telogen phase represents the hair shedding phase, as it culminates, after a few months, in the death and expulsion of the hair, making way for a new follicle in the anagen phase. During this phase, the hair does not grow but remains attached to the follicle, while the dermal papilla at the base of the epidermis is in a resting phase. The bulb detaches and moves forward until it reaches the superficial part of the hair follicle, the infundibulum, causing the hair to fall out spontaneously. We lose 60 to 100 hairs per day in this way. Simultaneously, a new hair bud appears, allowing a new hair shaft to form. The old hair, at the end of the telogen phase, can thus coexist with the new hair at the beginning of the anagen phase, which is located beneath it. This explains why, when the hair falls out, it is very quickly replaced at the follicular opening.On average, in a person without hair disorders, approximately 85% of the follicles are in the anagen phase and 15% are in the telogen phase. Body hair and eyelashes follow the same life cycles but have a relatively short growth phase and a longer resting phase, preventing excessive and inappropriate growth.

[0008] Hair loss is a normal process that occurs when a hair reaches the end of its life cycle. However, losing more than 100 hairs per day is considered abnormal and excessive. Episodes of hair dystrophy can then manifest as hair loss, but also as thinning hair and Thinning, slowed growth, or dry and brittle hair. Abnormal hair loss can be due to excessive production of dihydrotestosterone (which can cause androgenic alopecia) or a dysregulation of signaling pathways involved in the hair cycle (tq pCatenin / Wnt, TGF-, FGF, Shh, BMP, Notch...), responsible for slowing down or stimulating hair growth.

[0009] Furthermore, hair damage is often accompanied by scalp problems, such as a disruption in sebum production. Sebum, which protects the cuticle and gives shine to the hair, may then be produced in insufficient quantities, leading to cuticle damage, lifting of the scales, and resulting in brittle, dry, and rough hair. Conversely, excessive sebum production makes the hair greasy and can promote the growth of microorganisms, the appearance of dandruff and itching on the scalp, as well as hair loss.

[0010] Finally, the health of hair depends largely on a sufficient supply of nutrients to the hair follicle. Insufficient nutrients slow hair growth, weaken its structure, and negatively impact the formation of new, healthy hair. Nails are also sensitive to insufficient nutrients, leading to slowed growth and making them soft or brittle.

[0011] Nutritional intake therefore has a significant impact on the health and beauty of hair and nails. Sufficient intake of nutrients, vitamins, proteins, and trace elements helps maintain healthy hair and nails.

[0012] Solutions, including pharmaceutical ones, exist to combat hair loss or promote hair nourishment and growth. For example, minoxidil and finasteride are pharmaceutical treatments used to slow hair loss and can also be effective, to a lesser extent, in improving regrowth. However, these treatments cause significant side effects (oily hair, dandruff, skin irritation, libido problems) and do not maintain their effectiveness after treatment is stopped.

[0013] It is also known for its many cosmetic solutions. Indeed, in our society, which places great importance on appearance, hair is a symbol of beauty and strength, and its condition has a significant impact on self-esteem and the image we project. Accelerated hair loss is perceived as a sign of Premature aging can significantly affect the quality of life of those affected. Therefore, consumers are particularly sensitive to the effectiveness of such products and are constantly seeking new solutions to improve and stimulate growth, promote protection, nutrition, or hydration, or combat or even prevent hair loss. This is the purpose of the present invention. Summary of the invention

[0014] To address this need, the invention proposes a composition based on natural ingredients, namely a specific mixture of polyphenols, bamboo extract, and selenium as the active ingredient, forming the present composition. This specific mixture thus meets the requirements of the present invention and provides improved efficacy compared to existing solutions, particularly with regard to the efficacy of each ingredient taken individually.

[0015] Thus, the invention relates to a composition comprising, as an active ingredient, a mixture consisting essentially of: a mixture of polyphenols, a bamboo extract, and selenium.

[0016] This specific mixture of polyphenols, bamboo extract, and selenium, as an active ingredient, forming the composition according to the present invention, thus makes it possible to prevent and combat hair loss and promote regrowth, fight against oxidation, by a synergistic action of said three ingredients, which makes it possible to improve the known effects of certain ingredients taken individually, in particular polyphenols.

[0017] Indeed, the inventors have demonstrated through efficacy results that this mixture of three specifically selected ingredients presents a synergistic effect, particularly for its antioxidant effect, compared to the efficacy of each of these ingredients alone.

[0018] Furthermore, in the context of the present invention, the inventors have advantageously selected a bamboo shoot extract as the bamboo extract, which further improves efficacy compared to a bamboo extract, in particular an extract obtained from the mature stem of bamboo.

[0019] Thus, the inventors specifically selected an extract from young shoots of Bamboo for its enhanced properties in combination with selenium and the polyphenol blend.

[0020] Bamboo extract, particularly from the species Bambusa vulgaris, is thus derived from young shoots and preferably constitutes at least 5% of the active ingredient, namely the mixture of three ingredients, the percentage being given as a percentage of the total dry weight of the active ingredient. More preferably, the extract of young bamboo shoots of Bambusa vulgaris constitutes between 5% and 85% of the active ingredient, the percentage being given as a percentage of the total dry weight of the active ingredient. Even more preferably, the extract of young bamboo shoots of Bambusa vulgaris constitutes between 6% and 30% of the active ingredient, the percentage being given as a percentage of the total dry weight of the active ingredient.

[0021] According to another aspect of the invention, the polyphenol mixture is preferably obtained from a natural ingredient, namely an extract selected from an extract of vine, grape, tea, apple, cocoa, red berries, maritime pine, drupe fruit or nuts, and combinations thereof. These are of particular interest given the polyphenols they contain.

[0022] Advantageously, the polyphenol mixture comprises at least flavanol monomers. Preferably, the composition according to the invention thus comprises at least 15% flavanol monomers, measured by HPLC-DAD, the percentages being given on a dry weight basis relative to the total dry weight of the active ingredient. Most preferably, the composition according to the invention comprises between 15% and 95% flavanol monomers, more preferably between 20% and 90% flavanol monomers, and even more preferably between 25% and 85% flavanol monomers measured by HPLC-DAD, the percentages being given on a dry weight basis relative to the total dry weight of the active ingredient.

[0023] According to another preferred object of the invention, the composition according to the invention comprising this specific mixture of three ingredients, as active ingredients, has a flavanol monomer / bamboo extract ratio of between 0.17 and 19. Preferably the ratio of flavanol monomers to bamboo extract is between 2 and 12.5.

[0024] According to another preferred embodiment, the mixture forming the composition according to the invention also comprises selenium. This may be in an organic form such as selenomethionine, selenocystin, selenocysteine, or in an inorganic form such as than selenate or selenite. According to one variation, selenium can also be derived from selenium-enriched yeast, selenium-rich microalgae such as spirulina, Brazil nuts, brown rice, cashew nuts, mushrooms, wheat germ, or sunflower seeds. Finally, according to another variation, selenium can be a mixture of different selenium sources, such as selenium in an inorganic and / or organic form and / or a source chosen from selenium-enriched yeast, selenium-rich microalgae such as spirulina, Brazil nuts, brown rice, cashew nuts, mushrooms, wheat germ, or sunflower seeds.

[0025] Preferably, said mixture forming the composition according to the invention comprises at least 80 ppm of selenium, by dry weight relative to the total dry weight of the active ingredient.

[0026] According to another preferred object, the composition according to the invention is in any form suitable for oral administration. This composition may be a nutritional, pharmaceutical, or nutricosmetic composition.

[0027] Most preferably, said composition according to the invention is a nutricosmetic composition comprising said mixture, as an active ingredient, as described according to one of the preceding embodiments, and comprising at least one acceptable excipient.

[0028] The said composition according to the invention is advantageously presented in the form of a tablet, capsule, powder, encapsulated powder, gummies, soft capsule, drink, etc.

[0029] According to another aspect of the invention, the composition according to the invention aims to improve and promote the health of hair and / or nails, in particular hair and / or nail growth, and / or stimulate hair follicle growth and / or combat hair loss in an individual not suffering from a hair loss-related disease, and / or improve antioxidant capacity. Furthermore, the present invention also relates to a non-therapeutic use of said composition according to any of the embodiments previously described for: - to improve, promote hair growth, and / or - stimulate hair follicle growth, and / or - combat hair loss, and / or - improve antioxidant capacity, and / or - to increase hair shine in an individual not suffering from a hair loss-related disease.

[0030] According to another aspect of the invention, the composition according to the invention also aims to improve and promote hair growth and / or stimulate hair follicle growth and / or combat hair loss in an individual suffering from at least one hair loss-related disease, particularly an individual suffering from alopecia. In this context, the present invention relates to a composition according to any of the embodiments described above, for its use in improving and promoting hair growth and / or stimulating hair follicle growth and / or combating hair loss in an individual suffering from at least one hair loss-related disease, preferably alopecia.

[0031] Other features and advantages will become apparent from the detailed description of the invention, the examples and figures that follow. Brief description of the Figures

[0032] [Fig. 1] represents the results on the antioxidant capacity of the ingredients of the composition according to the invention, alone or in combination, namely the mixture of polyphenols from green tea, bamboo shoot extract and selenium yeast comprising 0.22% selenium.

[0033] [Fig. 2] represents the results on the antioxidant capacity of the ingredients of the composition according to the invention, alone or in combination, namely the mixture of polyphenols from grape, bamboo shoot extract and selenium yeast comprising 0.22% selenium.

[0034] [Fig. 3] represents the results on the antioxidant capacity of the ingredients of the composition according to the invention, alone or in combination, namely the mixture of polyphenols from green tea, bamboo shoot extract and pure selenium.

[0035] [Fig. 4] represents the comparative results on the antioxidant capacity of the composition according to the invention comprising an extract of bamboo shoot or an extract from the bamboo stem, compared to polyphenols from green tea alone.

[0036] [Fig. 5] represents the results on the proliferation of human hair follicle cutaneous papilla cells (HFDPC) in the presence or absence of the composition according to the invention.

[0037] [Fig. 6] represents the results on hair density in the anagen phase over the entire scalp.

[0038] [Fig. 7] represents the results on hair volume after treatment with the composition according to the invention, evaluated by a panel of experts. Detailed description of the invention

[0039] Definition

[0040] For the purposes of this invention, "active ingredient" or "active principle" refers to a mixture comprising a flavanol monomer, a bamboo extract, and selenium, acting synergistically to exert its effects on hair and / or scalp cells, particularly on dermal papilla cells, whether these effects are cosmetic or therapeutic. This mixture of three specifically selected ingredients acts as an active ingredient, enabling and enhancing the aforementioned effects compared to each ingredient taken individually, including an antioxidant effect, an effect to combat hair loss, and an effect to promote hair regrowth.

[0041] For the purposes of this invention, "bamboo extract" means at least one molecule, preferably a set of molecules, obtained from a species of bamboo selected from the group consisting of Bambusa vulgaris, Bambusa breviflora, Bambusa textilis, Phyllostachys nigra, Aralia nudicaulis and Lophatherum gracile.

[0042] For the purposes of this invention, "bamboo shoot extract" refers to a specific part of the bamboo plant, namely the young shoots. These young shoots are the tips of new bamboo stems, also called "turions," which are just emerging from the soil. They are characterized by not yet being fully lignified and fibrous, unlike the mature bamboo stem. Advantageously, the bamboo shoot extract contains silicon in monomeric and / or oligomeric form.

[0043] For the purposes of this invention, an "acceptable excipient" is any compound that facilitates the formulation of the composition and does not alter the nature of the biological activity of the mixture forming the composition according to the invention. An acceptable excipient may be a solvent, buffer, saline solution, plasticizer, lubricant, dispersion medium, absorption retardant or enhancer, flow agent, isotonic agent, antioxidant, or chelating agent. For example, it may be a pharmaceutically acceptable excipient, chosen according to the desired pharmaceutical form and route of administration. The excipient will thus be selected based on the route of administration, for example, suitable for oral administration. It may also be a cosmetically acceptable excipient, that is, one that does not cause discomfort to the user, such as redness, tightness, or tingling. For example, the excipient can be chosen from among a preservative, an antioxidant, a stabilizer, or a spray-drying agent. Finally, it can be an excipient acceptable in nutritional products, such as dextrins, maltodextrin, sugars, glycerin, fats, vegetable waxes, vegetable oils, proteins, peptides, alginates, phospholipids, polyols (for example, sorbitol or maltitol), starch, cellulose, calcium or magnesium carbonate, or gum arabic.

[0044] Composition

[0045] The present invention therefore relates to a composition comprising, as an active ingredient, a mixture consisting essentially of: a mixture of polyphenols, a bamboo extract, and selenium.

[0046] This specific blend, comprising a mixture of polyphenols, bamboo extract, and selenium as the active ingredient, forms the composition according to the invention and helps prevent and combat hair loss while promoting regrowth. Furthermore, the synergistic effect of these three ingredients enhances the antioxidant capacity and overall effectiveness in preventing and combating hair loss compared to the effectiveness of each ingredient individually.

[0047] Furthermore, the present composition according to the invention is particularly suitable for promoting overall hair health, especially for hair weakened by extrinsic or intrinsic factors. Moreover, such a synergistic action from the mixture of polyphenols, bamboo extract, and selenium is surprising and unexpected in light of prior art knowledge, as no synergistic effect has been reported from the combination of these three ingredients.

[0048] Thus, the polyphenol blend, a carefully selected mixture of natural polyphenolic compounds, in combination with bamboo extract and selenium, promotes hair health. Several polyphenol blends are particularly suitable for inclusion in this formulation, including blends of polyphenols derived from natural ingredients such as vine, grape, tea, apple, cocoa, berries, maritime pine, drupe, or nut extracts, and combinations thereof. Preferably, the polyphenol blend is derived from from the vine or green tea, very preferably green tea.

[0049] The polyphenols derived from the vine, in particular from Vitis vinifera, especially from grape seeds and skins, are particularly rich in proanthocyanidins, resveratrol and flavonoids, thus helping to protect hair follicles from oxidative stress and promoting hair growth.

[0050] As an example, a grape seed extract rich in flavanol monomers, particularly suitable for inclusion in this composition, can be obtained using the following process: a. Hydroalcoholic extraction of dried grape seeds in a liquid heated to 60°C b. Liquid / solid separation c. Filtration through a suitable membrane, followed by permeate passing through an adsorption chromatography column d. Purification using an adsorption resin e. Washing with water f. Hydroethanolic elution g. Concentration under vacuum and dealcoholization h. Pasteurization of the concentrated aqueous extract i. Spray drying

[0051] Tea, particularly green tea, is especially rich in catechins, including epicatechol gallate (EGCG). EGCG has been studied in particular for its role in inhibiting the production of dihydrotestosterone (DHT), a hormone associated with hair loss, and for stimulating hair follicles.

[0052] A green tea extract rich in flavanol monomers can be obtained by the following process: a. Aqueous extraction of green tea leaves in water heated to 85°C b. Liquid / solid separation of the liquid tea leaf extract by filtration c. Vacuum concentration and dealcoholization d. Purification of the aqueous extract by chromatography on adsorption resin e. Washing with water f. Vacuum evaporation and concentration of the aqueous extract g. Pasteurization of the concentrated aqueous extract h. Drying of the purified extract by spray drying and sieving

[0053] Apple extracts also contain procyanidins, a type of condensed tannin. Procyanidins promote hair growth by prolonging the anagen phase of the hair growth cycle.

[0054] Cocoa is rich in flavanols, which have been linked to increased blood circulation. Improved blood circulation to the scalp is beneficial for the health of hair follicles and thus promotes hair growth.

[0055] Red fruits such as berries are also rich in anthocyanins and ellagic acid, having antioxidant properties of particular interest to help protect hair and scalp from environmental damage.

[0056] Maritime pine bark extract includes oligomeric proanthocyanidins (OPCs) that strengthen hair follicles and improve hair growth.

[0057] Extracts from drupes (such as plums, cherries and apricots) or nuts (such as almonds and walnuts) are rich in various polyphenols, including lactic acid and gallic acid, which again contribute to overall hair health.

[0058] Finally, a combination of these polyphenolic extracts is particularly preferred to obtain a broad-spectrum polyphenol blend that harnesses and enhances the benefits of each source. Thus, the specific polyphenols included in the blend can be tailored to target different aspects of hair health, such as promoting growth, strengthening, and protecting against oxidative damage.

[0059] Thus, the polyphenol mixture is preferentially obtained from a natural ingredient chosen from an extract of vine, grape, tea, apple, cocoa, red fruits, maritime pine, drupe fruits or nuts, and their combination.

[0060] Advantageously, the polyphenol mixture comprises at least flavanol monomers. Preferably, the composition according to the invention comprises at least 15% flavanol monomers measured by HPLC-DAD, the percentages being given on a dry weight basis relative to the total dry weight of the active ingredient. Most preferably, the composition according to the invention comprises between 15% and 95% flavanol monomers, more preferably between 20% and 90% flavanol monomers, and even more preferably between 25% and 85% flavanol monomers measured by HPLC-DAD, the percentages being given on a dry weight basis relative to the total dry weight of the active ingredient.

[0061] This mixture of polyphenols, particularly when it includes at least 15% of Flavanol monomers are advantageously selected for their ability to act in synergy with the other ingredients in the composition, particularly bamboo shoot extract and selenium, thus improving the overall effectiveness of the hair treatment, the antioxidant effect, and consequently promoting hair health, hair growth and limiting hair loss.

[0062] According to another object, the mixture forming the composition according to the invention also comprises a bamboo extract, preferably a bamboo shoot extract, but also selenium.

[0063] The extract of young bamboo shoots, in particular of the species Bambusa vulgaris, is particularly rich in bioactive compounds, thus contributing to improving the overall effectiveness of the active ingredient within the meaning of the present invention and participating in the synergistic effect observed by the inventors.

[0064] According to another preferred embodiment of the present invention, the bamboo (Bambusa vulgaris) extract is derived from young shoots and constitutes at least 5% of the active ingredient, the percentage being given as a percentage of the total dry weight of the active ingredient. More preferably, the Bambusa vulgaris young shoot extract constitutes between 5% and 85% of the active ingredient, the percentage being given as a percentage of the total dry weight of the active ingredient. Even more preferably, the Bambusa vulgaris young shoot extract constitutes between 6% and 30% of the active ingredient, the percentage being given as a percentage of the total dry weight of the active ingredient.

[0065] In a particularly preferred manner, the composition according to the invention comprising this specific mixture of three ingredients, as an active ingredient, has a flavanol monomer / bamboo extract ratio of between 0.17 and 19, even more preferably said ratio is between 2 and 12.5.

[0066] Finally, the third ingredient in the mixture is selenium. This can be obtained from several sources. In the context of the present invention, it can be in an organic form such as selenomethionine, selenocystin, or selenocysteine; or in an inorganic form such as selenate or selenite. According to one embodiment, the selenium can also be obtained from selenium-enriched yeast, selenium-enriched microalgae such as spirulina, Brazil nuts, brown rice, cashew nuts, mushrooms, wheat germ, or sunflower seeds. Finally, the selenium can also be a mixture of different sources of selenium, especially selenium in an inorganic and / or organic form and / or a source chosen from selenium-enriched yeast, selenium-enriched microalgae such as spirulina, Brazil nuts, brown rice, cashew nuts, mushrooms, wheat germ or sunflower seed.

[0067] Selenium is an essential trace element that plays a crucial role in various physiological processes in the human body, including hair health. Selenium is a component of selenoproteins, including glutathione peroxidases, which have antioxidant properties and help protect cells, including hair follicles, from oxidative damage. Thus, selenoproteins also contribute to hair structure and growth.

[0068] According to another preferred source, selenium, regardless of its origin or form, must represent at least 80 ppm of selenium, by dry weight relative to the total dry weight of the active ingredient. More preferably, between 80 ppm and 3800 ppm, by dry weight relative to the total dry weight of the active ingredient.

[0069] Thus, prior art does not teach us any synergistic effect associated with the combination of these three ingredients, thereby allowing us to address the disadvantages of prior art.

[0070] According to another embodiment of the present invention, the active ingredient can combine with the three ingredients described above a fourth ingredient, thus constituting a new active ingredient. This fourth ingredient, in combination with the three ingredients described above, can be a source of silica, advantageously in organic or inorganic form, particularly from horsetail, bamboo, nettle, or oats; or from a synthetic source such as Mesoporosil.

[0071] According to another preferred embodiment, the composition according to the invention is presented in a form suitable for oral administration. More preferably, said composition is a nutricosmetic composition.

[0072] Nutricosmetic compositions are nutritional supplements designed to promote the health and appearance of skin, hair, and nails through oral administration, as opposed to local administration, such as topical or scalp application. This nutricosmetic composition intended for oral administration includes an active ingredient, namely the specific blend mentioned above, which provides nutritional support that, in turn, improves the appearance of the skin, hair, and nails. cosmetics, particularly hair health. Thus, the said active ingredient, which consists of the specific mixture, namely a mixture of polyphenols, selenium and bamboo extract, acts synergistically to promote hair health, in particular to combat hair loss and / or promote regrowth.

[0073] This nutricosmetic composition is intended to be administered orally, and is specially designed to improve the health of hair and / or nails.

[0074] Furthermore, the nutricosmetic composition according to the invention advantageously comprises at least one acceptable excipient which facilitates the formulation and administration of said mixture of active ingredients.

[0075] These excipients may include the following: - Bulking agents such as microcrystalline cellulose, maltodextrin, and gum arabic, used to give volume and consistency to the composition. - Binders such as starch, gelatin or hydroxypropylcellulose, used to ensure the adhesion and formulation of ingredients in tablet or capsule form. - Disintegrants such as croscarmellose sodium or sodium starch glycolate, used to help the tablet or capsule break down in the digestive system. - Lubricating agents such as magnesium stearate or silicon dioxide, used to prevent ingredients from sticking to equipment during manufacturing. - Antioxidant agents such as ascorbic acid or tocopherols, used to prevent the oxidation of active ingredients. - Preservatives such as potassium sorbate or sodium benzoate, used to extend the shelf life of the composition.

[0076] In this context, where the active ingredient as defined in the present invention is combined with at least one acceptable excipient, the quantity of each ingredient, taken individually, for example bamboo extract, may be modified accordingly. Therefore, the quantities of each ingredient constituting the active ingredient are expressed as a percentage of the total dry weight of the active ingredient without any acceptable excipient or other carrier.

[0077] Thus, the composition according to the invention can be formulated in various dosage forms suitable for oral administration, in particular in the form of capsules, tablets, powders, soft capsules, gummies, etc.

[0078] Use

[0079] According to another aspect of the invention, the composition according to the invention is particularly suitable for treatment, especially for the health of hair and / or nails. Thus, the invention relates to a non-therapeutic use of the composition according to the invention to improve and promote hair growth, and / or stimulate hair follicle growth, and / or combat hair loss in an individual not suffering from a hair loss-related disease, and / or to improve antioxidant capacity.

[0080] Indeed, hair follicles are highly sensitive to oxidative stress due to their rapid cell turnover and exposure to various environmental factors. Oxidative stress can therefore impact hair fibers both before emergence (oxidative metabolism, smoking, UV radiation, and inflammation of microbial, polluting, or irritating origin) and after emergence (UV radiation, chemical attacks). These negative impacts on hair follicle cells can lead to premature aging, reduced hair growth, and hair loss, and can also disrupt the normal hair growth cycle. High levels of oxidative stress can induce a premature entry into the catagen phase and thus shorten the duration of the anagen phase.Damage to proteins caused by oxidative stress, particularly keratin, can also lead to increased hair porosity and greater fragility, making hair brittle and dull. Therefore, the composition according to the invention is particularly suitable for the aforementioned uses.

[0081] According to another object, the present composition is also suitable for an individual suffering from at least one hair loss-related condition, such as alopecia. Thus, the invention also relates to said composition according to the invention for its therapeutic use to improve and promote hair growth and / or stimulate hair follicle growth and / or combat hair loss in an individual suffering from at least one hair loss-related condition, preferably alopecia.

[0082] The invention is now illustrated by non-limiting examples of compositions according to the invention and by results. Examples

[0083] Example 1: Preparation of a grape seed extract rich in flavanol monomers.

[0084] The extract is obtained using the following process: - Hydroalcoholic extraction (70% water, 30% ethanol) of 40 kg of dried grape seeds in 400 kg of liquid heated to 60°C - Liquid / solid separation, then the aqueous extract is passed through a < 15kD membrane - The permeate was then passed through a C18 adsorption chromatographic column - Purification on adsorption resin 10 BV to 2 BV / h - Water wash 4 BV to 2 BV / h - Hydroethanolic elution with an 80% v / v solution at 1 Bv / h with 2 Bv - Vacuum concentration and dealcoholization under 60 mbar at 40°C - Pasteurization of the concentrated aqueous extract at a temperature of 85°C for 10 minutes - Spray drying

[0085] This grape seed extract thus obtained (El) comprises at least 95% total polyphenols (DO-280), of which 30% are flavanol monomers (measured by UHPLC-DAD), the quantities being given relative to the dry weight of the extract. It can be characterized in terms of polyphenols as described in Table 1 below.

[0086] Example 2: Preparation of a green tea extract rich in flavanol monomers.

[0087] The extract is obtained using the following process: - Aqueous extraction of 60 kg of green tea leaves in 600 L of water heated to 85°C for 2 hours - Liquid / solid separation of the liquid extract of tea leaves by filtration - Vacuum concentration and dealcoholization under 60 mbar at 40°C - Purification of the aqueous extract by chromatography on adsorption resin with ethanol elution using an 80% v / v solution - Water wash 4 BV to 2 BV / h - Evapoconcentration of ethanol under vacuum and concentration of the aqueous extract at a temperature preferably of 60°C and at a pressure below 90m bar - Pasteurization of the concentrated aqueous extract at a temperature of 85°C for 10 minutes - Drying of the purified extract by spray drying and sieving

[0088] Such a green tea extract thus obtained (E2) comprises at least 98% total polyphenols (DO-280), of which 80% are flavanol monomers (measured by UHPLC-DAD), the quantities being given relative to the dry weight of the extract. It can be characterized in terms of polyphenols as described in Table 1 below.

[0089] Example 3: Preparation of red grape marc extract.

[0090] The extract is obtained using the following process: - Hydroalcoholic extraction - Red grape marc extract (E3) - 10kg of dry pomace, hydro-alcoholic extraction at 30% V / V, solid-liquid ratio 1 / 10, agitation at 350 rpm at 80°C for 4 hours - Solid-liquid separation by 20 µm filtration - Concentration under vacuum at 40°C - Spray drying

[0091] The grape extract thus obtained (E3) is characterized in Table 1 below.

[0092] Example 4: Preparation of a purified red grape pomace extract.

[0093] The extract is obtained using the following process: - Extraction at 80°C for 4 hours at 50% v / v EtOh ratio 1 / 10 - 10kg of dry pomace, hydro-alcoholic extraction at 30% V / V, solid-liquid ratio 1 / 10, agitation at 350 rpm at 80°C for 4 hours - Solid-liquid separation by 20 µm filtration - Concentration under vacuum at 40°C - Purification on adsorption resin 20 BV to 2 BV / h - Water wash 4 BV to 2 BV / h - Hydroethanolic elution with an 80% v / v solution at 1 Bv / h with 2 Bv - Vacuum concentration and dealcoholization under 200 mbar at 40°C - Freeze-drying

[0094] The grape extract thus obtained (E4) is characterized in Table 1 below.

[0095] Example 5: Preparation of a white grape marc extract.

[0096] The extract is obtained using the following process: - 125kg of white grape pomace, hydro-alcoholic extraction at 30% V / V, solid-liquid ratio 1 / 10, agitation at 350 rpm at 80°C for 4 hours - Solid-liquid separation by 20 µm filtration - Vacuum concentration / dealcoholization at 100 mbar and 40°C - Vacuum concentration and dealcoholization at 200 mbar and 40°C - Spray drying.

[0097] The grape extract thus obtained (E5) is also characterized in Table 1 below.

[0098] [Table 1]

[0099] Example 6 according to the invention: Active ingredients according to the invention in powder form

[0100] Table 2 below presents several examples of an active ingredient according to the invention, comprising extracts obtained from the processes described previously in Examples 1 to 5 and characterized in Table 1 by simply mixing the different extracts in the quantities indicated. The percentages of each ingredient constituting the active ingredient are given as a dry weight of the total dry weight of the active ingredient.

[0101] [Table 2]

[0102] The various active ingredients thus obtained comprise at least 15% flavanol monomers, at least 5% bamboo extract, a flavanol monomer / bamboo extract ratio of between 0.17 and 19 and at least 80 ppm selenium, by dry weight of the total dry weight of the active ingredient.

[0103] The active ingredients are obtained by mixing the constituents under conventional conditions known to those skilled in the art. Alternatively, the active ingredient may be obtained from an extract or a mixture of extracts.

[0104] The active ingredients described in this example 6 in powder form can then serve as a basis for compositions described below.

[0105] Examples 7 according to the invention: Capsule-type composition

[0106] Several examples of capsule-type compositions including the active ingredient according to the invention are presented in Table 3 below.

[0107] [Table 3]

[0108] Other ingredients can thus be added to the composition according to the invention to complement the properties of the composition, however without additional effect, in particular synergistic effect on hair health, such as vitamins, minerals, amino acids and excipients such as magnesium stearate, calcium carbonate, sodium carbonate, acacia fiber, talc, maltodextrin, silicon dioxide, modified starches, cellulose, microcrystalline cellulose, cellulose gum, polysorbates.

[0109] The composition is obtained by mixing the constituents under standard conditions known to those skilled in the art, and then encapsulated under similarly standard conditions. The recommended dosage for the examples in Table 3 is 1 capsule per day.

[0110] Example 8 according to the invention: Tablet-type composition

[0111] Example 8 is a tablet-type composition comprising an active ingredient according to the invention, in particular described in Table 3, and the following excipients: sodium carboxymethyl starch, microcrystalline cellulose, gelatin, talc, magnesium stearate; as well as the following colorants: titanium dioxide, yellow iron oxide, red iron oxide; and the following film coating: glycerol, sodium lauryl sulfate, macrogol 6000, hypromellose, magnesium stearate.

[0112] The composition according to example 8 is obtained by mixing the constituents, and then the 1000mg tablet is prepared according to standard manufacturing conditions. known to those in the trade.

[0113] Example 9 according to the invention: Chewing gum type composition

[0114] Example 9 is a food supplement, in the form of chewing gum comprising an active ingredient according to the invention, in particular described in Table 3, as well as pectin, citric acid, sorbitol, water, and maltitol.

[0115] It is possible to add to the composition according to the invention other ingredients having their own efficacy (without effect on the hair within the meaning of the invention), such as vitamins, minerals, amino acids, aromas and colorants, as shown in the examples in Table 4 below.

[0116] The composition according to example 9 is obtained by mixing the constituents and then the chewing gum is manufactured according to the classic manufacturing conditions known to the person skilled in the art.

[0117] [Table 4]

[0118] Example 10 according to the invention: Capsule-type mole composition

[0119] Example 10 consists of the composition described in Example 6 in the form of a softgel capsule, containing 1g of fish oil rich in EPA / DHA and 12mg of lutein. The recommended dosage is 1 capsule per day.

[0120] Example 11 according to the invention: Composition in the form of a beverage

[0121] Example 11 is a 50 ml beverage containing the equivalent of 300 mg of the composition according to the invention. The mixture may consist of 250 mg of green tea extract, 40 mg of bamboo shoot extract, and 10 mg of selenium-enriched yeast (0.2% selenium). The extracts may be microencapsulated. Concentrated fruit juices, flavorings, colorings, preservatives, and sweeteners may be added to the composition. The recommended dosage is 1 to 2 shots of 25 ml per day.

[0122] Example 12 according to the invention: Composition in the form of yogurt

[0123] Example 12 is a yogurt-based formula, to be consumed 2 to 3 times a day. The composition is detailed in Table 5.

[0124] [Table 5]

[0125] Example 13 according to the invention: Dog kibble type composition

[0126] Example 13 is a kibble composition for a 20 kg dog fed 300 g of kibble per day, providing: - 6.6 mg per kg of body weight from a polyphenol source as described in Examples 1 to 3, - 462 pg of bamboo shoot extract per kg of body weight, and - 1.46 pg of selenium per kg of body weight.

[0127] Example 14 outside the scope of the invention: Composition with a bamboo leaf extract

[0128] This example is a composition comprising a green tea extract as characterized in Table 1 (E2), a bamboo leaf extract and selenium.

[0129] Examples 15 outside the scope of invention: Composition outside the scope of invention

[0130] Table 6 below presents several examples of composition including extracts from the processes described above in examples 1 and 2 or characterized in Table 1. The mixtures do not include at least one of the following four characteristics, namely: at least 15% flavanol monomers, at least 5% bamboo extract, a flavanol monomer / bamboo extract ratio between 0.17 and 19 and at least 80 ppm of selenium.

[0131] [Table 6]

[0132] Efficacy test

[0133] Efficacy test 1 - Evaluation of the effect of the composition according to the invention on the oxygen radical absorbing capacity (ORAC)

[0134] Reactive oxygen species, or free radicals, are molecules that can severely damage lipids, proteins, and DNA. They are generated by a multitude of endogenous and environmental factors, potentially leading to progressive damage to cellular structures, including hair. Oxidative stress can impact both pre-emergent hair fibers (oxidative metabolism, smoking, UV radiation, and inflammation of microbial, pollutant, or irritant origin) and post-emergent fibers (UV radiation, chemical aggressions). Although the body possesses endogenous antioxidant defense mechanisms, these diminish with age, potentially creating an imbalance between free radicals and antioxidant defenses. Thus, antioxidants present in food can play an important role in overall health, including hair health.

[0135] The objective of this test is therefore to compare the antioxidant capacity of the composition according to the invention with the ingredients taken individually or in combination two by two.

[0136] To this end, antioxidant activity was measured in vitro using a colorimetric reaction according to the ORAC method, an analytical method used to determine the antioxidant activity of natural substances found in dietary supplements, certain pharmaceutical agents, or food ingredients. The ORAC test, well-known to those skilled in the art, evaluates the ability of the antioxidants in a sample to limit the oxidation of fluorescein, a fluorescent probe sensitive to oxidation, by peroxide radicals generated by an oxidizing component, AAPH. The oxidation of fluorescein is accompanied by a decrease in fluorescence over time. Antioxidants inhibit the oxidation reaction and thus temporarily delay the decrease in fluorescence. The antioxidant potential is then determined by the difference in area under the curve between the sample being measured and an antioxidant control, Trolox®. One ORAC unit is equivalent to 1 mol of Trolox® per gram of sample.

[0137] In a 96-well black plate, the following quantities were loaded into each well: 25 µL of the sample to be tested, diluted solutions of Trolox for the standard range (between 5 and 30 pM) or APHA phosphate buffer for the blank (pH 7.5, 85 mM), and 150 µL of fluorescein (8 nM). After an incubation period of 15 min at 37°C, 25 µL of AAPH (153 mM) was added to each well to initiate the reaction. Subsequently, fluorescence was monitored for 20 min (excitation 485 nm, emission 520 nm). The resulting ORAC value is expressed as pmol Trolox / g extract.

[0138] Synergistic effects were assessed using a three-way analysis of variance (ANOVA), followed by a multiple comparison test (Tukey's high-stress test). Finally, a Mann-Whitney U test was performed to assess significance between two groups.

[0139] The results are presented in Fig. 1 and Fig. 2 as mean ± SEM. P values ​​< 0.05 denote statistical significance.

[0140] The results thus demonstrate that the mixture of polyphenols from green tea [Fig. 1] and grape [Fig. 2] possesses a fairly significant antioxidant capacity, unlike bamboo extract and selenium when tested alone, or together (bamboo + selenium). However, when the polyphenols from green tea or grape are added, the extract of Bamboo and selenium significantly increase effectiveness. The inventors observed a synergistic effect associated with the combination of these three ingredients.

[0141] The combination of the 3 ingredients (Example B in Table 2) thus increases the ORAC capacity of the green tea polyphenol + bamboo combination by 53% (Fig. 1; p<0.001), and that of the green tea polyphenol + selenium combination by 40% (Fig. 1; p<0.001). The same is true for the combination of the 3 ingredients (Example C in Table 2) when the polyphenols are derived from grapes, whose antioxidant capacity improves the ORAC capacity of the bamboo extract + polyphenol combination by 23% (Fig. 2; p<0.001) and that of the selenium-enriched yeast + polyphenol combination by 16.6% (Fig. 2; p<0.001).

[0142] These results therefore demonstrate a synergistic effect of the ingredients of the composition according to the invention on antioxidant capacity, as summarized in Table 7 below describing examples of formula calculations giving synergistic results on antioxidant capacity.

[0143] [Table 7]

[0144] Furthermore, the inventors demonstrated that the beneficial impact of selenium-enriched yeast on the antioxidant capacity of polyphenols observed in Figure 1 is indeed due to its selenium content. The same experiment was performed with pure selenium (<99.5%) as a replacement for the yeast. As with the selenium-enriched yeast, the results show that this alternative source of selenium significantly increases the antioxidant capacity of tea polyphenols (Fig. 3; p<0.05), and that the combination of selenium, bamboo extract, and polyphenols further increases the ORAC capacity of the latter (Fig. 3; p<0.001). These data demonstrate that selenium, regardless of its source, is at least partially responsible for the synergistic effect and not another compound in the yeast.

[0145] Efficacy test 2 - Antioxidant effect of the composition according to the invention compared to a composition not related to the invention

[0146] This trial, following the protocol described in trial 1, aims to demonstrate that the synergistic effect of the combination of the three ingredients depends on the part of the bamboo used, and not on its silica content as one might have expected from the teaching of the prior art. Indeed, we compared the ORAC capacity of tea polyphenols alone to the combination of tea polyphenols, selenium, and either bamboo shoot or bamboo stem extract, both standardized to 75% silica. Surprisingly, the inventors found that combining the ingredients with bamboo shoot extract significantly improves the ORAC capacity of the polyphenols alone (Fig. 4; p<0.001), whereas combining them with bamboo stem extract has no significant impact on the ORAC value compared to tea polyphenols.

[0147] These results described in Figure 4 demonstrate that the silica from bamboo, present in the same quantity in both extracts, is not responsible for the synergistic effect of the combination of the three ingredients, but for other molecules specific to the bamboo shoot.

[0148] Efficacy Test 3 - Evaluation of the effect of the composition according to the invention on proliferative efficacy on human hair follicle dermal papilla cells (HFDPC)

[0149] Hair regeneration originates from hair follicle stem cells (HFSCs) and dermal papilla cells (DPCs). The latter form the cellular layer around the bulb and are an important cell reservoir for hair regeneration.

[0150] The objective of this test was to compare the proliferation of dermal papilla cells from human follicles in the presence or absence of the composition according to the invention (Example B of Table 2).

[0151] To this end, primary cultures isolated from human scalp dermis (HFDPC) were incubated at 37°C with 5% CO2 in growth medium for HFDPC. The cells (6 cell replicas per condition) were exposed to the composition according to the invention, either with or without it, for 48 hours. At the end of this incubation period, the cell nuclei were immunolabeled with the fluorochrome DAPI and detected by epifluorescence microscopy, then counted using ImageJ software.

[0152] The results are expressed as the average number of cells per area (Fig. 5) and show the percentage increase in the number of cells compared to the control group (Table 8, below).

[0153] [Table 8]

[0154] The composition according to the invention, namely the combination of polyphenols, bamboo shoot extract, and selenium, significantly increases the number of cells per unit area compared to the untreated condition (p<0.001), thus improving cell density by 23%. These results demonstrate the effectiveness of the invention on the proliferation of cells responsible for hair growth in vitro.

[0155] Efficacy test 4 - Evaluation of the effect of the composition according to the invention on hair growth and health in women with acute telogen effluvium.

[0156] This study aims to evaluate the effectiveness of a composition according to the invention, namely consisting essentially of: Flavanol monomers: 73.2% Bamboo shoot extract: 6.4%, Selenium: 90.3 ppm, on hair growth, loss, density, elasticity and shine in healthy women with acute telogen effluvium (33 women), compared to a placebo group (31 women).

[0157] This composition is administered once a day at breakfast for 12 weeks (1 capsule of 280mg).

[0158] The main inclusion criteria are as follows: healthy Caucasian women aged 18 to 65 years with non-pathological transient acute telogen effluvium (duration less than 6 months) not taking any treatment for hair loss for at least 3 months before the study not taking any treatment (oral or topical) that may interfere with hair growth or loss for the duration of the study not dyeing hair for 4 weeks before the start of the study and for the duration of the study.

[0159] The main objective of this study is to confirm the improvement in hair growth using a phototrichogram (TrichoScan) after 84 days.

[0160] The secondary objectives of this study are to confirm: the improvement in hair growth using a phototrichogram (TrichoScan) after 42 days, Increased hair density and thickness after 42 and 84 days of supplementation, reduced hair loss after 42 and 84 days, improved hair length after 84 days, and increased hair shine and elasticity after 84 days.

[0161] The phototricogram aims to assess hair growth / loss. Digital photos were taken immediately after shaving a section of the scalp (0-1.8 cm) and again two days later. The two photos were compared using software capable of determining whether the hair was in the anagen (growth) or telogen (resting) phase.

[0162] The hair pulling test is used to assess hair loss. Gentle traction is applied to 60 hairs at 3 different locations on the scalp. If more than 10 hairs are pulled per test, the subject has telogen effluvium.

[0163] The spectrocolorimetry test aims to evaluate hair color and shine by assessing light reflection at a specific angle. The measurement (taken at 5 points near the hair roots) of light reflection is performed at an 8-degree angle of incidence relative to the perpendicular to the surface. The higher this value, the shinier the hair.

[0164] The results are presented in Table 9 for women receiving the composition according to the invention and Table 10 for women receiving the placebo, as well as [Fig. 6] and [Fig. 7], (*p<0.05; **p<0.01; ***p<0.001 vs. T0; sp<0.05; ss p<0.01; sss pc0.001 vs. Placebo).

[0165] [Table 9] 0166] [Table 10]

[0167] These results show that the composition according to the invention allows for an increase in hair density in the anagen phase [Fig. 6], an improvement in hair shine and volume [Fig. 7] as well as a reduction in hair loss, which shows that the composition according to the invention induces better hair health with promoted hair regrowth / regeneration.

[0168] Efficacy Test 5 - Evaluation of the effect of a composition according to the invention on the hair growth and health in women wishing to improve the growth and strength of their hair.

[0169] This real-life study aims to evaluate the effectiveness of a composition according to the invention on the growth, strength, density, hydration, shine and beauty of hair in 40 healthy women.

[0170] This composition according to the invention consists essentially of 26% monomers, 145ppm of selenium and 10.6% bamboo shoot extract, and is administered once a day in the morning for 3 months (1 capsule of 170mg).

[0171] The inclusion criteria are as follows: Healthy French women, aged 25 to 45 Being dissatisfied with the quality of their hair (having poor growth, strength, shine) Not to be pregnant, breastfeeding, menopausal or perimenopausal, or to have given birth less than 6 months ago Do not take any hair / nail products (treatments, dietary supplements, essential oils) for at least 2 months before the study. Do not follow long-term drug treatment Do not use any treatment (oral or topical) that may interfere with hair growth or loss for the duration of the study. Not suffering from psoriasis or scalp eczema Not having made any changes to your hair care routine in the 2 months preceding the study and throughout the entire duration of the study

[0172] The main objective of this study is to confirm the improvement in hair quality after 3 months of supplementation using satisfaction questionnaires.

[0173] To this end, questionnaires are completed at T0 (before the start of treatment), T30, T60 and T90 days in order to confirm: Improving hair growth Improving hair strength Improving hair density Improving hair shine Improving hair hydration Hair beauty enhancement The perceived effectiveness of the composition

[0174] The results are presented in Table 11 below.

[0175] [Table 11]

[0176] These results show that the composition according to the invention improves hair growth, strength, shine, density, hydration, and beauty after only one month of supplementation. These various parameters are further improved after three months of supplementation. Overall, 90% of women found the formula effective in improving the overall beauty of their hair after three months of supplementation.

Claims

Demands

1. Composition comprising, as an active ingredient, a combination of ingredients consisting essentially of: a mixture of polyphenols, a bamboo extract, and selenium

2. Composition according to the preceding claim, characterized in that the bamboo extract is a bamboo shoot extract.

3. Composition according to any one of the preceding claims, characterized in that the mixture of polyphenols is obtained from a natural ingredient selected from an extract of vine, grape, tea, apple, cocoa, red fruits, maritime pine, drupe fruits and nuts.

4. Composition according to any one of the preceding claims, characterized in that the mixture of polyphenols comprises at least flavanol monomers.

5. Composition according to the preceding claim, characterized in that the active ingredient consists essentially of: at least 15% flavanol monomers, measured by HPLC-DAD, and / or at least 5% bamboo shoot extract, and / or at least 80 ppm selenium, the quantities being given in dry weight relative to the total dry weight of the active ingredient.

6. Composition according to any one of claims 4 or 5, characterized in that the ratio of flavanol monomers and bamboo extract is between 0.17 and 19.

7. Composition according to any one of the preceding claims, characterized in that the composition is in a form suitable for oral administration.

8. Nutricosmetic composition comprising the composition according to one of the preceding claims and at least one acceptable excipient.

9. Non-therapeutic use of a composition according to any one of claims 1 to 8, to improve, promote hair growth, and / or stimulate hair follicle growth and / or increase the shine of hair in an individual not suffering from a hair loss-related disease.

10. Composition according to any one of claims 1 to 7, for its use in improving, promoting hair growth and / or stimulating hair follicle growth and / or combating hair loss, in an individual suffering from at least one hair loss-related disease.

11. Composition according to any one of claims 1 to 7, for its use for its antioxidant effects.

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