Dual-optical detection apparatus and detection method

Abstract

The present application relates to a dual-optical detection apparatus and a detection method. The dual-optical detection apparatus comprises: a light source apparatus, used for emitting excitation light; a function switching apparatus, capable of switching between a fluorescence detection state and a chemiluminescence detection state; and a detection apparatus, used for detecting fluorescence and chemiluminescence in a reaction solution. A zoomable light-collection apparatus is located between the function switching apparatus and the detection apparatus, and the zoomable light-collection apparatus can change a light-collection diameter thereof. The dual-optical detection apparatus can perform fluorescence detection and chemiluminescence detection, so that required detection can be selected according to requirements, thereby reducing the cost of a device, reducing the space occupied by the device, and facilitating rapid detection by an inspector. The zoomable light-collection apparatus can effectively improve the accuracy of a detection result.

Description

A dual-optical detection device and detection method Technical Field

[0001] This application relates to the field of in vitro diagnostic technology, and in particular to a dual-optical detection device and detection method. Background Technology

[0002] In the field of clinical testing and analysis, many tests can be rapidly performed using fluorescence detection and chemiluminescence detection technologies. The principle of fluorescence detection is that antigen-antibody binding produces a fluorescent substance. This substance emits fluorescence under the action of a special substrate solution, and this fluorescence can be quantitatively detected and analyzed under specific optical paths, thereby achieving the detection and analysis of the target analyte.

[0003] Chemiluminescent immunoassay is a novel labeled immunoassay technique that combines chemiluminescence or bioluminescence systems with an immune reaction to detect trace amounts of antigens or antibodies. Its detection principle is similar to radioimmunoassay and enzyme immunoassay, but the difference lies in using luminescent substances instead of radioactive nuclides or enzymes as labels, and directly measuring the amount of light emitted by the luminescent substance itself.

[0004] In practical applications, it is often necessary to select chemiluminescence immunoassay or fluorescence immunoassay to detect samples based on specific needs and occasions. Existing dual-optical detection devices can perform both fluorescence detection and chemiluminescence detection. However, when the content of the analyte is low, users cannot accurately determine whether the analyte is present, resulting in inaccurate detection results.

[0005] Summary of the Invention

[0006] One objective of this application is to provide a dual-optical detection device to at least solve one of the aforementioned technical problems.

[0007] To achieve the above objectives, the first aspect of this application provides a dual optical detection device, comprising:

[0008] A light source device used to emit excitation light;

[0009] The function switching device can switch between fluorescence detection mode and chemiluminescence detection mode;

[0010] A detection device used to detect fluorescence and chemiluminescence in the reaction solution.

[0011] A zoom light-collecting device is located between the function switching device and the detection device, and the zoom light-collecting device can change its light-collecting diameter.

[0012] Optionally, the zoomable light-collecting device can change its light-collecting diameter according to the intensity of the fluorescence or the chemiluminescence; and / or the zoomable light-collecting device can focus the fluorescence or the chemiluminescence onto the detection device; or

[0013] The zoom light-collecting device adjusts its light-collecting diameter according to the type of luminescent liquid; or

[0014] The zoom light-collecting device adjusts its light-collecting diameter according to the fluorescent material; or

[0015] The zoom and light-gathering device is connected to the function switching device.

[0016] Optionally, when the intensity of the fluorescence or chemiluminescence exceeds a minimum preset value, the zoom light-collecting device increases its light-collecting diameter; and / or when the intensity of the fluorescence or chemiluminescence exceeds a maximum preset value, the zoom light-collecting device decreases its light-collecting diameter.

[0017] If the luminescent liquid has a high efficiency in generating chemiluminescence, the zoom light-collecting device reduces its light-collecting diameter; if the luminescent liquid has a low efficiency in generating chemiluminescence, the zoom light-collecting device increases its light-collecting diameter; or

[0018] If the fluorescent material has a high fluorescence quantum yield, the zoom light-collecting device reduces its light-collecting diameter; if the fluorescent material has a low fluorescence quantum yield, the zoom light-collecting device increases its light-collecting diameter.

[0019] Optionally, the zoom light-collecting device includes a first housing, multiple lenses, and a driving component. The multiple lenses are disposed within the first housing and spaced apart along the transmission directions of the fluorescence and the chemiluminescence. The number of driving components is at least one, and the driving component is connected to the lenses to adjust the spacing between at least two adjacent lenses. The multiple lenses can focus the fluorescence and the chemiluminescence onto the detection device.

[0020] Optionally, the plurality of lenses includes at least one lens group, the lens group including a first convex lens, a first concave lens and a second convex lens arranged in sequence, wherein the first convex lens is disposed close to the detection device, the second convex lens is disposed close to the function switching device, and the distance between the first concave lens and the first convex lens and the second convex lens is adjustable.

[0021] Optionally, at least two sets of the lens groups are provided, wherein in two adjacent sets of the lens groups, the second convex lens of one set of the lens groups is the first convex lens of the other set of the lens groups.

[0022] Optionally, the function switching device includes at least two optical channels and a working position;

[0023] Each of the optical channels can be located at the working position, so that at least one of the optical channels can transmit the chemiluminescence to the detection device, so that the function switching device is in the chemiluminescence detection state, and so that at least one of the optical channels can transmit the excitation light of a preset wavelength to the reaction solution and the fluorescence to the detection device, so that the function switching device is in the fluorescence detection state.

[0024] Optionally, at least one of the optical channels includes a filter and a dichroic mirror, wherein the filter allows excitation light of a preset wavelength to enter the optical channel, and the dichroic mirror allows fluorescence of the preset wavelength to pass through, so that the fluorescence enters the detection device.

[0025] Optionally, the function switching device includes a second housing and a mounting bracket. The mounting bracket includes the at least two optical channels. The mounting bracket is movably disposed on the second housing so that each optical channel can be located at the working position. The optical channel located at the working position can transmit the excitation light and fluorescence of a preset wavelength or can transmit the chemiluminescence.

[0026] Optionally, the mounting bracket is rotatably disposed within the second housing, and the at least two optical channels are spaced apart along the rotation center of the mounting bracket; and / or

[0027] The second housing includes a first light inlet, a first light outlet, and a first light outlet. The optical channel includes a second light inlet, a second light outlet, and a second light outlet. When the optical channel is in the working position, the first light inlet and the second light inlet are directly opposite each other, and the first light inlet and the second light outlet are directly opposite each other. The excitation light enters the second housing through the first light inlet, and the excitation light of a preset wavelength exits the second housing through the first light outlet. The fluorescence and the chemiluminescence enter the second housing through the first light outlet and exit the second housing through the first light outlet.

[0028] Optionally, the mounting frame includes a frame body rotatably connected to the second housing, and the optical channel further includes an optical tube detachably connected to the frame body, wherein the at least one optical tube is provided with the filter and the dichroic mirror.

[0029] Optionally, the function switching device further includes a first drive mechanism, which is connected to the second housing and the mounting bracket and drives the mounting bracket to rotate.

[0030] Optionally, the second light outlet and the second light inlet / outlet are located on the same straight line, and the second light inlet is perpendicular to the second light outlet;

[0031] The filter is disposed at the second light inlet, and the dichroic mirror is disposed at the light channel, and is at a 45° angle to both the second light inlet and the second light outlet.

[0032] Optionally, the light source device includes a light source and an incident optical fiber, the incident optical fiber being used to transmit the excitation light emitted by the light source to the function switching device; and / or

[0033] The detection device includes a detector and a receiving optical fiber. The receiving optical fiber is used to transmit the fluorescence and chemiluminescence output by the function switching device to the detector, and the detector is used to detect the fluorescence and chemiluminescence; and / or

[0034] The dual-optical detection device also includes a second driving mechanism, which drives the detection box containing the reaction liquid to move in three-dimensional space.

[0035] Optionally, the dual optical detection device further includes an apochromatic focusing device, which is located downstream of the function switching device. The apochromatic focusing device is used to focus the beams formed by the excitation light of different wavelengths onto the same test site.

[0036] Another objective of this application is to provide a detection method to at least solve one of the aforementioned technical problems.

[0037] To achieve this objective, the second aspect of this application adopts the following technical solution:

[0038] A detection method, wherein the dual optical detection device performs the detection method, the detection method comprising the following steps:

[0039] Depending on the detection method required by the reaction solution in the detection box, switch the function switching device to the corresponding fluorescence detection state or chemiluminescence detection state;

[0040] The zoom light-collecting device changes its light-collecting diameter according to the intensity of the detected fluorescence or chemiluminescence; or the zoom light-collecting device changes its light-collecting diameter according to the type of luminescent liquid; or the zoom light-collecting device changes its light-collecting diameter according to the type of fluorescent substance.

[0041] The reaction solution was tested.

[0042] As can be seen from the above, the technical solution provided in this application, when fluorescence detection is required, the function switching device switches to the fluorescence detection state, the light source device generates excitation light, and the function switching device can transmit the excitation light of a preset wavelength to the detection box. When the reaction liquid in the detection box contains the substance to be detected, the reaction liquid generates fluorescent substance, and the fluorescent substance emits fluorescence under the action of the substrate liquid. The function switching device can transmit the fluorescence generated by the reaction liquid in the detection box to the detection device, so that the detection device can detect the fluorescence, that is, the fluorescence is quantitatively detected and analyzed, and fluorescence detection is realized.

[0043] When chemiluminescence detection is required, the function switching device switches to the chemiluminescence detection state. When the reaction solution in the detection box contains the substance to be detected, the substance to be detected emits chemiluminescence. The function switching device can transmit the chemiluminescence emitted by the reaction solution to the detection device so that the detection device can detect the chemiluminescence. The chemiluminescence is quantitatively detected and analyzed, thus realizing chemiluminescence detection.

[0044] A zoomable light-collecting device can change its light-collecting diameter. Therefore, if the content of the analyte is low and it is impossible to definitively determine whether the analyte is present, the zoomable light-collecting device can be used to change its light-collecting diameter to obtain a stronger light signal and determine whether the analyte is present. For example, if increasing the light-collecting diameter of the zoomable light-collecting device allows the detection device to obtain a stronger light signal corresponding to the analyte, then the analyte is present. If increasing the light-collecting diameter of the zoomable light-collecting device does not allow the detection device to detect the light signal corresponding to the analyte, then the reaction solution does not contain the analyte. Alternatively, if increasing the light-collecting diameter of the zoomable light-collecting device does not change the light signal intensity, then it may be an interference signal, and the reaction solution does not contain the analyte.

[0045] The dual-optical detection device can perform both fluorescence and chemiluminescence detection, allowing inspectors to select the required detection method as needed, reducing equipment costs and space requirements, and facilitating rapid testing. At the same time, the zoomable light-collecting device can effectively improve the accuracy of the detection results, especially when the content of the analyte is low. Attached Figure Description

[0046] Figure 1 is a schematic diagram of the dual optical detection device provided in an embodiment of this application;

[0047] Figure 2 is a schematic diagram of the structure of a partial function switching device (removing a set of optical channels) provided in an embodiment of this application;

[0048] Figure 3 is an exploded view of the function switching device (removing a set of optical channels) provided in an embodiment of this application;

[0049] Figure 4 is a cross-sectional view of the function switching device provided in an embodiment of this application;

[0050] Figure 5 is a schematic diagram of the optical path inside the dual optical detection device provided in the embodiment of this application;

[0051] Figure 6 is a schematic diagram of the structure of a partial function switching device (removing a set of optical channels) provided in an embodiment of this application;

[0052] Figure 7 is a magnified view of part A in Figure 3;

[0053] Figure 8 is a schematic diagram of the structure of the light tube provided in the embodiment of this application.

[0054] Figure 9a is a schematic diagram of the apochromatic focusing device and detection box provided in an embodiment of this application;

[0055] Figure 9b is a schematic diagram of the apochromatic focusing device provided in an embodiment of this application;

[0056] Figure 10 is a schematic diagram of the zoom light-collecting device provided in an embodiment of this application;

[0057] Figure 11a is a schematic diagram of multiple lenses (the first concave lens is located in the first position) provided in an embodiment of this application;

[0058] Figure 11b is a schematic diagram of multiple lenses (the first concave lens is located in the second position) provided in an embodiment of this application;

[0059] Figure 11c is a schematic diagram of multiple lenses (the first concave lens is located in the third position) provided in an embodiment of this application;

[0060] Figure 12 is a perspective view of the zoom light-gathering device provided in the embodiment of this application.

[0061] In the diagram: 1. Bracket; 2. Light source device; 21. Light source; 22. Incident optical fiber; 3. Function switching device; 31. Second housing; 311. First light inlet; 312. First light inlet / outlet; 313. First light outlet; 315. Lower housing; 316. Top cover; 317. Observation window; 318. Sealing cover; 32. Mounting bracket; 321. Optical channel; 3211. Filter; 3212. Dichroic mirror; 3213. Second light inlet; 3234. Second light inlet / outlet; 3235. Second light outlet; 3236. Optical tube; 3237. Limiting block; 322. Frame; 3221. Limiting platform; 3222. Shaft hole; 323. Baffle; 324. Bolt; 33. First drive mechanism; 331. First drive unit; 332. Driven gear; 34. Fixed shaft; 4. Detection device; 41. Detector; 42. Receiving optical fiber; 5. Zooming light receiving device; 51. First housing; 52. Lens; 521. Lens group; 524. First convex lens; 525. First concave lens; 526. Second convex lens; 53. Driving component; 6. Apochromatic focusing device; 61. Biconvex lens; 62. Concave lens; 63. Plano-convex lens; 7. Excitation light; 8. Fluorescence; 10. Detection box; Detailed Implementation

[0062] The technical solution of this application will be further described below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely for explaining this application and not for limiting it. Furthermore, it should be noted that, for ease of description, only the parts relevant to this application are shown in the accompanying drawings, not all of them.

[0063] This application defines certain directional terms. Unless otherwise stated, the directional terms used, such as "up," "down," "left," "right," "inner," and "outer," are used for ease of understanding and therefore do not constitute a limitation on the scope of protection of this application.

[0064] In this application, unless otherwise expressly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature being directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature being directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0065] In the description of this application, unless otherwise expressly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0066] This embodiment provides a dual-optical detection device for fluorescence and chemiluminescence detection of exosomes such as blood, nasal swabs, and pharyngeal swabs, thereby reducing equipment costs, minimizing equipment space requirements, and facilitating rapid testing by examiners.

[0067] As shown in Figure 1, the dual-optical detection device provided in this embodiment includes a light source device 2, a function switching device 3, a zoomable light-collecting device 5, and a detection device 4. The light source device 2 emits excitation light 7, and the function switching device 3 can switch between fluorescence detection and chemiluminescence detection states. The detection device 4 detects fluorescence 8 and chemiluminescence in the reaction solution. The zoomable light-collecting device 5 is located between the function switching device 3 and the detection device 4, and its light-collecting diameter can be changed.

[0068] When the function switching device 3 is switched to the fluorescence detection state, it can transmit the excitation light 7 of the preset wavelength in the excitation light 7 to the reaction liquid in the detection box 10, and can transmit the fluorescence 8 generated by the reaction liquid in the detection box 10 to the detection device 4. When the function switching device 3 is switched to the chemiluminescence detection state, it can transmit the chemiluminescence generated by the reaction liquid to the detection device 4.

[0069] When fluorescence detection is required, the function switching device 3 switches to fluorescence detection mode. The light source device 2 generates excitation light 7, and the function switching device 3 can transmit the excitation light 7 of a preset wavelength to the detection box 10. When the reaction solution in the detection box 10 contains the substance to be detected, the reaction solution emits fluorescence 8. The function switching device 3 can transmit the fluorescence 8 generated by the reaction solution in the detection box 10 to the detection device 4, so that the detection device 4 can detect the fluorescence 8, that is, the fluorescence 8 is quantitatively detected and analyzed, thus realizing fluorescence detection. For example, in fluorescence detection technology, the analyte and the fluorescent substance are mixed to form a reaction solution. The analyte may be attached with a marker, which can bind to the fluorescent substance. When the reaction solution is irradiated with excitation light of a specific wavelength, the fluorescent substance can absorb the excitation light and emit fluorescence 8 under the action of the absorbed excitation light, thereby realizing fluorescence detection. When chemiluminescence detection is required, the function switching device 3 switches to the chemiluminescence detection state. When the reaction solution in the detection box 10 contains the substance to be detected, the substance to be detected emits chemiluminescence. The function switching device 3 can transmit the chemiluminescence emitted by the reaction solution to the detection device 4, so that the detection device 4 can detect the chemiluminescence. The chemiluminescence is quantitatively detected and analyzed, thus realizing chemiluminescence detection. The zoom light-collecting device 5 can change its light-collecting diameter. Therefore, if the content of the analyte is low and it is not possible to clearly determine whether the analyte is present, the zoom light-collecting device 5 can be used to change its light-collecting diameter to obtain a stronger light signal and determine whether the analyte is present. For example, if the detection device 4 can obtain a stronger light signal corresponding to the analyte after the zoom light receiving device 5 increases its light receiving diameter, then it can be determined that the analyte is present. If the detection device 4 still cannot detect the light signal corresponding to the analyte after the zoom light receiving device 5 increases its light receiving diameter, it means that the reaction liquid does not contain the analyte. Or, if the light signal remains at its original intensity after the zoom light receiving device 5 increases its light receiving diameter, it may be an interference signal, and the reaction liquid does not contain the analyte.

[0070] The dual-optical detection device provided in this embodiment can perform both fluorescence and chemiluminescence detection, allowing inspectors to select the required detection method as needed, reducing equipment costs and space requirements, and facilitating rapid testing. Simultaneously, the zoomable light-collecting device 5 effectively improves the accuracy of the detection results, especially when the content of the analyte is low.

[0071] Optionally, the dual-optical detection device may further include a bracket 1, a light source device 2, a function switching device 3, and a detection device 4, all connected to the bracket 1. For example, the light source device 2, the function switching device 3, and the detection device 4 may be connected to the bracket 1 by welding, gluing, threaded connection, or other methods. The bracket 1 can support the detection box 10 containing the reaction liquid, so that the reaction liquid inside the detection box 10 can be detected.

[0072] In this embodiment, a reaction solution is formed by adding a substrate solution, a diluent, and other reagents to the exosomes. The reaction solution is then injected into the detection box 10, and the detection box 10 is placed on the support 1. The reaction solution can then be detected by fluorescence or chemiluminescence using a dual optical detection device.

[0073] Optionally, the light source device 2 may include a light source 21 and an incident optical fiber 22, the incident optical fiber 22 being used to transmit the excitation light 7 emitted by the light source 21 to the function switching device 3. For example, the light source 21 is full-spectrum light, thereby providing excitation light 7 of various wavelengths. One end of the incident optical fiber 22 may be connected to the light source 21, and the other end may be connected to the function switching device 3, thereby realizing the transmission of the excitation light 7.

[0074] For example, the detection device 4 may include a detector 41 and a receiving optical fiber 42. The receiving optical fiber 42 is used to transmit the fluorescence 8 and chemiluminescence output by the function switching device 3 to the detector 41, and the detector 41 is used to detect the fluorescence 8 and chemiluminescence. Optionally, one end of the receiving optical fiber 42 may be connected to the detector 41, and the other end may be connected to the function switching device 3, thereby realizing the transmission of fluorescence 8 and chemiluminescence. The detector 41 may include a silicon photomultiplier tube, a photonic detector 41, or a photomultiplier tube. The detector 41 using a silicon photomultiplier tube, a photonic detector, or a photomultiplier tube has high sensitivity, and during detection, it achieves ultrafast and high-sensitivity detection of optical signals at the millisecond level.

[0075] As shown in Figures 2-5, the function switching device 3 may include at least two optical channels 321 and a working position. Each optical channel 321 can be located in the working position, so that at least one optical channel 321 can transmit chemiluminescence to the detection device 4, putting the function switching device 3 into a chemiluminescence detection state, and at least one optical channel 321 can transmit excitation light 7 of a preset wavelength to the reaction solution and fluorescence 8 to the detection device 4, putting the function switching device 3 into a fluorescence detection state. That is, at least one optical channel 321 can transmit excitation light 7 and fluorescence 8 of a preset wavelength. When the optical channel 321 that can transmit excitation light 7 and fluorescence 8 of a preset wavelength is located in the working position, the function switching device 3 is in a fluorescence detection state; at least one optical channel 321 can transmit chemiluminescence. When the optical channel 321 that can transmit chemiluminescence is located in the working position, the function switching device 3 is in a chemiluminescence detection state, thereby enabling the function switching device 3 to perform fluorescence detection and chemiluminescence detection.

[0076] Optionally, although the function switching device 3 may include at least two optical channels 321, the same optical channel 321 can perform both fluorescence detection and chemiluminescence detection, as long as it can transmit chemiluminescence and fluorescence 8, and can transmit excitation light 7. Of course, in other optional embodiments, the same optical channel 321 may only perform fluorescence detection or only perform chemiluminescence detection. The type of detection that the same optical channel 321 can perform depends on the wavelength of the light allowed to pass through the optical channel 321. For example, the optical channel 321 allows light with a wavelength of λ1 to pass through in order to transmit light with a wavelength of λ1 to the detection device 4. When the wavelengths of both chemiluminescence and fluorescence 8 are λ1, then the optical channel 321 can perform both fluorescence detection and chemiluminescence detection.

[0077] Detector 41 cannot identify the wavelength of light. During fluorescence detection, the type of fluorescence 8 produced by the substance to be detected is specific, that is, the wavelength of fluorescence 8 is specific. To ensure that a light channel 321 transmits only fluorescence 8 of a preset wavelength, and thus allows detector 41 to detect only the intensity of fluorescence 8 produced by the substance to be detected, optionally, at least one light channel 321 may include a filter 3211 and a dichroic mirror 3212. The filter 3211 allows excitation light 7 of a preset wavelength to enter the light channel 321, and then the light channel 321 transmits the excitation light 7 of the preset wavelength to the reaction liquid in the detection box 10. The dichroic mirror 3212 allows fluorescence 8 of the preset wavelength to pass through, so that fluorescence 8 enters the detection device 4. At the same time, the dichroic mirror 3212 blocks the excitation light 7 reflected back by the reaction liquid from passing through the dichroic mirror 3212, thereby preventing the excitation light 7 from being transmitted to the detection device 4, reducing noise, and ensuring the accuracy of the detection results.

[0078] In this embodiment, multiple optical channels 321 can be set, and different wavelengths of excitation light 7 and / or different wavelengths of fluorescence 8 are transmitted in different optical channels 321, so that the dual optical detection device can detect different objects to be detected.

[0079] Optionally, at least one optical channel 321 can transmit chemiluminescence of a preset wavelength to the detection device 4, thus also transmitting the chemiluminescence to be detected to the detection device 4. Optionally, in at least one optical channel 321, a dichroic mirror 3212 or a filter 3211 is provided on the path of chemiluminescence transmission to allow only one preset wavelength of chemiluminescence to be transmitted to the detection device 4.

[0080] In other optional embodiments, at least one optical channel 321 can transmit chemiluminescence of all wavelengths to the detection device 4. When the function switching device 3 needs to switch to the chemiluminescence detection state, chemiluminescence detection is performed through the optical channel 321. For chemiluminescence detection, it is not necessary to excite the reaction liquid with excitation light 7. When the reaction liquid contains the analyte, it can generate self-luminescence of a specific wavelength under the action of the luminescent liquid, i.e., chemiluminescence. Therefore, the optical channel 321 only needs to transmit chemiluminescence. Thus, it is not necessary to filter the excitation light 7 with a dichroic mirror 3212, nor is it necessary to transmit the excitation light 7 with a preset wavelength with a filter 3211. Therefore, at least one optical channel 321 can transmit chemiluminescence of all wavelengths to the detection device 4.

[0081] In this embodiment, six optical channels 321 are provided, but the number of optical channels 321 is not limited to this; there may be more than six or less than six.

[0082] As shown in Figures 2 and 3, exemplarily, the function switching device 3 may include a second housing 31 and a mounting bracket 32. Optionally, the working position is located within the second housing 31, and the mounting bracket 32 ​​may include at least two optical channels 321. The mounting bracket 32 ​​is movably disposed on the second housing 31 so that each optical channel 321 can be located in the working position. The optical channel 321 in the working position can transmit fluorescence 8 and excitation light 7 of a preset wavelength or can transmit chemiluminescence. By moving the mounting bracket 32, the optical channels 321 are switched. Thus, when the optical channel 321 transmitting excitation light 7 of a preset wavelength and fluorescence 8 is in the working position, the function switching device 3 is in fluorescence detection state; when the optical channel 321 transmitting chemiluminescence is in the working position, the function switching device 3 is in chemiluminescence detection state.

[0083] Optionally, the mounting bracket 32 ​​is rotatably disposed within the second housing 31, and at least two optical channels 321 are spaced apart along the rotation center of the mounting bracket 32. The working position is located on the rotation path of the optical channel 321. When the mounting bracket 32 ​​rotates around the rotation center, each optical channel 321 can be located at the working position.

[0084] Of course, in other alternative embodiments, the optical channel 321 can also be positioned at and away from the working position by linear movement. For example, multiple optical channels 321 are arranged circumferentially. The multiple optical channels 321 can be located on regular patterns, such as on the same circle or polygons such as quadrilaterals or pentagons, or they can be located on irregular patterns. The working position is located inside the pattern formed by the multiple optical channels 321, such as the approximate center position of the pattern, so that each optical channel 321 is located at the working position and there is no interference between them when the optical channels 321 move.

[0085] As shown in Figure 4, optionally, the second housing 31 may include a first light inlet 311, a first light outlet 312, and a first light outlet 313. The light channel 321 may include a second light inlet 3213, a second light outlet 3234, and a second light outlet 3235. When the light channel 321 is in the working position, the first light inlet 311 and the second light inlet 3213 are directly opposite each other, and the first light inlet 311 and the second light outlet 3235 are directly opposite each other. Excitation light 7 enters the second housing 31 through the first light inlet 311, and excitation light 7 with a preset wavelength is emitted from the second housing 31 through the first light outlet 312. Fluorescence 8 and chemiluminescence enter the second housing 31 through the first light outlet 312 and are emitted from the second housing 31 through the first light outlet 313, thereby realizing the transmission of light.

[0086] As shown in Figures 1 and 4, optionally, the incident fiber 22 is connected to the first light inlet 311 so that the excitation light 7 enters the second housing 31 and the optical channel 321 through the first light inlet 311. The receiving fiber 42 is connected to the first light outlet 313 so that the fluorescence 8 and chemiluminescence in the optical channel 321 enter the receiving fiber 42 through the first light outlet 313.

[0087] Optionally, the second light-emitting port 3235 and the second light-in / out port 3234 are located on the same straight line, and the second light-in port 3213 is perpendicular to the second light-emitting port 3235. This facilitates light entering the second housing 31 and the light channel 321, and also facilitates light exiting from the second housing 31 and the light channel 321. It is understood that the first light-emitting port 313 and the first light-in / out port 312 are also located on the same straight line, and the first light-in port 311 is also perpendicular to the first light-emitting port 313.

[0088] As shown in Figures 4 and 5, further, a filter 3211 is disposed at the second light inlet 3213, and a dichroic mirror 3212 is disposed at the light channel 321, forming a 45° angle with both the second light inlet 3213 and the second light outlet 3234. After passing through the filter 3211, the excitation light 7 is filtered into monochromatic excitation light of a preset wavelength. After reflection by the dichroic mirror 3212, the transmission direction of the preset wavelength excitation light 7 rotates by 90°, allowing it to be transmitted to the second light outlet 3234. Fluorescence 8 and chemiluminescence enter the light channel 321 through the first light outlet 312 and the second light outlet 3234, and after linear transmission (passing through the dichroic mirror 3212), are transmitted to the first light outlet 313 and the second light outlet 3235. Simultaneously, the excitation light 7 cannot pass through the dichroic mirror 3212 and is reflected by it, thus avoiding noise pollution.

[0089] Optionally, as shown in Figures 3-6, the mounting frame 32 may include a frame 322, which is rotatably connected to the second housing 31. The optical channel 321 may also include an optical tube 3236. In this embodiment, the second light outlet 3235, the second light inlet 3213, and the second light inlet / outlet 3234 are all located on the optical tube 3236. The optical tube 3236 is detachably connected to the frame 322, and at least one optical tube 3236 contains a filter 3211 and a dichroic mirror 3212 (the filter 3211 and dichroic mirror 3212 are omitted in Figures 2, 3, 6, and 7). When the required optical channel 321 is not available in the function switching device 3, the required optical channel 321 can be provided by replacing the optical tube 3236 with the required filter 3211 and dichroic mirror 3212.

[0090] As shown in Figure 7, optionally, the light tube 3236 can be roughly rectangular.

[0091] As shown in Figures 6-8, the optical tube 3236 can be detachably connected to the frame 322 via bolts 324 or other connecting parts. As shown in Figures 7 and 8, the optical channel 321 may also include a limiting block 3237, which is connected to the outer side of the optical tube 3236. Specifically, limiting blocks 3237 are provided on opposite sides of the optical tube frame 322. A limiting platform 3221 is provided on the frame 322, and the limiting blocks 3237 can be placed on the limiting platform 3221.

[0092] The mounting bracket 32 ​​may also include a baffle 323, which can be detachably connected to the bracket body 322 by bolts 324. The baffle 323 can stop the limiting block 3237, thereby preventing the limiting block 3237 from detaching from the limiting platform 3221. There can be one or more baffles 323, as long as they can fix the limiting block 3237 to the limiting platform 3221. In this embodiment, one limiting block 3237 is provided with two baffles 323.

[0093] Of course, in other alternative embodiments, the light tube 3236 can be detachably connected to the frame 322 by snap-fit, such as a snap-fit ​​protrusion on the light tube 3236 and a snap-fit ​​groove on the frame 322, which can snap-fit ​​with the snap-fit ​​protrusion.

[0094] As shown in Figures 2-4, the function switching device 3 may also include a first drive mechanism 33, which is connected to the second housing 31 and the mounting bracket 32 ​​and drives the mounting bracket 32 ​​to rotate.

[0095] For example, the first drive mechanism 33 may include a first drive unit 331, a driving gear (not shown in the figure), and a driven gear 332. The first drive unit 331 is connected to the second housing 31. The first drive unit 331 may be a stepper motor or a motor, etc. The driving gear is connected to the output end of the first drive unit 331, and the driven gear 332 meshes with the driving gear. The first drive unit 331 drives the driving gear to rotate, thereby the driving gear 332 drives the driven gear 332 to rotate. The mounting bracket 32 ​​is connected to the driven gear 332, and thus rotates with the driven gear 332. For example, the bracket body 322 is connected to the driven gear 332.

[0096] As shown in Figure 4, optionally, the mounting bracket 32 ​​has a shaft hole 3222, specifically, the shaft hole 3222 is formed in the bracket body 322. As shown in Figure 3, a fixed shaft 34 is connected to the second housing 31, the fixed shaft 34 passes through the shaft hole 3222, and a rolling bearing is provided between the shaft hole 3222 and the fixed shaft 34. When the mounting bracket 32 ​​rotates, the mounting bracket 32 ​​rotates around the fixed shaft 34. The fixed shaft 34 can improve the stability of the rotation of the mounting bracket 32, thereby improving the detection accuracy.

[0097] As shown in Figure 3, optionally, an observation window 317 may be provided on the second housing 31. A sealing cover 318 is detachably connected to the observation window 317. When it is necessary to check whether there is a fault inside the second housing 31, the sealing cover 318 can be opened. After the inspection is completed, the sealing cover 318 can be closed to prevent dust from entering the second housing 31.

[0098] The second housing 31 may include a lower housing 315 and an upper cover 316, with the upper cover 316 covering the lower housing 315. A fixed shaft 34 may be connected to the second housing 31, and a mounting bracket 32 ​​may be accommodated within the lower housing 315.

[0099] For example, the first light inlet 311 and the first light outlet 312 are provided on the lower housing 315, and the first light outlet 313 is provided on the upper cover 316.

[0100] In actual testing, users select appropriate luminescent liquids or fluorescent substances based on different detection targets. The wavelength ranges of chemiluminescence or fluorescence 8 produced by different types of reagents are also different. After determining the detection target, the user can determine the luminescent liquid or fluorescent substance and the wavelength of fluorescence 8 or chemiluminescence. Then, the function switching device 3 will select the corresponding optical channel 321 according to the wavelength range selected by the user, and use the optimal wavelength excitation light 7 as the experimental light to irradiate the reaction solution. The filter 3211, dichroic mirror 3212, etc., realize the selection and control of the optimal excitation light 7, while avoiding interference from stray light.

[0101] As shown in Figures 1, 9a, and 9b, optionally, the dual-optical detection device may further include an apochromatic focusing device 6. The apochromatic focusing device 6 is located between the function switching device 3 and the detection box 10. For example, the apochromatic focusing device 6 is connected to the function switching device 3, such as at the first light inlet / outlet port 312 of the second housing 31, with the light inlet end of the apochromatic focusing device 6 communicating with the first light inlet / outlet port 312. The detection box 10 may be located below the apochromatic focusing device 6.

[0102] The apochromatic focusing device 6 is used to focus the excitation light 7 onto the reaction liquid. This ensures that when switching between different optical channels 321, the beams formed by excitation light 7 of different wavelengths are all focused on the same test point (as shown in Figure 9b, the three lines represent three beams of different wavelengths). This minimizes differences in chromatic aberration, astigmatism, field curvature, and other indicators between the reaction liquids, ensuring that the testing environment remains unchanged after the function switching device 3 switches to different functions. This achieves compatibility between different testing systems, providing a hardware foundation for the repeatability of the dual-optical detection device and thus improving detection accuracy. For example, the same test point specifically refers to a test point at a consistent height from the surface of the reaction liquid.

[0103] Chromatic aberration refers to the fact that light of different wavelengths converges at different distances after passing through a conventional convex lens. The longer the wavelength, the longer the focal length, and the shorter the wavelength, the shorter the focal length. As shown in Figure 9b, the apochromatic focusing device 6 can focus three wavelengths (red, green, and blue) of light onto a single plane. Therefore, after passing through the apochromatic device 6, the beams formed by excitation light 7 of different wavelengths will all converge at the same test point.

[0104] For example, the apochromatic focusing device 6 includes a biconvex lens 61, a concave lens 62, and a plano-convex lens 63 arranged sequentially away from the function switching device 3. The convex surface of the plano-convex lens 63 faces the function switching device 3. The biconvex lens 61 and the plano-convex lens 63 are made of the same material. The refractive index of the concave lens 62 is higher than that of the biconvex lens 61 and the plano-convex lens 63. The biconvex lens 61 and the plano-convex lens 63 are made of low-dispersion materials such as fluorite, crown glass, AD glass, ED glass, or UD glass.

[0105] As shown in Figures 2 and 10-12, optionally, the dual optical detection device may further include a zoom light-receiving device 5, which may be located between the function switching device 3 and the detection device 4. For example, the zoom light-receiving device 5 is connected to the function switching device 3. For instance, the zoom light-receiving device 5 is connected to the first light-emitting port 313 of the second housing 31, and the light-inlet end of the zoom light-receiving device 5 is connected to the first light-emitting port 313. The receiving optical fiber 42 is connected to the light-emitting end of the zoom light-receiving device 5.

[0106] The zoom-collecting device 5 can change the collecting diameter of its collecting side according to the light intensity of the fluorescence 8 or chemiluminescence. As mentioned above, in this embodiment, when the content of the analyte is low, if the zoom-collecting device 5 increases the collecting diameter, thereby increasing the collecting area, the detection device 4 can obtain a stronger light signal corresponding to the analyte, thus confirming the presence of the analyte. If the detection device 4 still cannot detect the light signal corresponding to the analyte after the zoom-collecting device 5 increases the collecting diameter, it indicates that the reaction solution does not contain the analyte. Alternatively, if the light signal remains at its original intensity after the zoom-collecting device 5 increases the collecting diameter, it may be an interference signal, and the reaction solution does not contain the analyte. When the zoom-collecting device 5 changes the collecting diameter of its collecting side according to the light intensity of the fluorescence 8 or chemiluminescence, it can be used to detect whether a specific analyte is present, or to detect the concentration of the analyte. The concentration of the analyte can be calculated by combining the collecting diameter with the concentration.

[0107] Of course, in other optional embodiments, the light-collecting diameter of the zoom light-collecting device 5 can be adjusted to the maximum before detection begins. When the light intensity that the detection device 4 can obtain is too high, the light-collecting diameter of the zoom light-collecting device 5 can be reduced. In this way, when the content of the substance to be detected is low, the detection device 4 can accurately detect the light signal.

[0108] Optionally, when the light intensity of fluorescence 8 or chemiluminescence exceeds the minimum preset value, the zoom light-collecting device 5 increases its light-collecting diameter to determine whether it contains the analyte to be detected.

[0109] When the light intensity of fluorescence 8 or chemiluminescence exceeds the maximum preset value, the zoom light receiving device 5 reduces its light receiving diameter to prevent the light intensity value of fluorescence 8 or chemiluminescence from being too high, so that the detection device 4 cannot detect the result.

[0110] In other optional embodiments, for chemiluminescent immunoassay, the zoomable light-collecting device 5 adjusts its collecting diameter according to the type of luminescent liquid. The luminescent liquid used for a specific analyte is determined, and the efficiency of the chemiluminescence produced by that specific luminescent liquid is known. Therefore, when adjusting the collecting diameter of the zoomable light-collecting device 5 according to the luminescent liquid, the zoomable light-collecting device 5 can be adjusted before detection. That is, for a specific analyte or luminescent liquid, the collecting diameter of the zoomable light-collecting device 5 is determined. This allows the relationship between the light intensity value detected by the detector 4 and the concentration of the analyte to be determined in advance, thus making the concentration calculation of the analyte more convenient.

[0111] Specifically, if the luminescent liquid has a high efficiency in producing chemiluminescence, the zoom light-collecting device 5 reduces its light-collecting diameter; if the luminescent liquid has a low efficiency in producing chemiluminescence, the zoom light-collecting device 5 increases its light-collecting diameter. This effectively avoids the situation where the concentration of the analyte is low, and the low efficiency of the luminescent liquid in producing chemiluminescence leads to the inability to detect the concentration of the analyte or to determine whether the analyte is present.

[0112] For fluorescence detection technology, the zoom light-collecting device 5 adjusts its light-collecting diameter according to the type of fluorescent substance.

[0113] The fluorescent material used for a specific analyte is known, and the fluorescence quantum yield (Yf, which is the ratio of the number of photons emitted by the fluorescent material after absorption to the number of photons absorbed by the excitation light) of the specific fluorescent material is known. Therefore, when adjusting the collecting diameter of the zoom collecting device 5 according to the fluorescent material, the zoom collecting device 5 can be adjusted before detection. That is, for a specific analyte or fluorescent material, the collecting diameter of the zoom collecting device 5 is determined. In this way, the relationship between the light intensity value detected by the detection device 4 and the concentration of the analyte can be determined in advance, thus making the concentration calculation of the analyte more convenient.

[0114] Specifically, if the fluorescence quantum yield of the fluorescent substance is high, the zoom light-collecting device 5 reduces its light-collecting diameter; if the fluorescence quantum yield of the fluorescent substance is low, the zoom light-collecting device 5 increases its light-collecting diameter, thereby effectively avoiding the inability to detect the concentration of the analyte or determine whether the analyte is present when the concentration of the analyte is low due to the low fluorescence quantum yield of the fluorescent substance.

[0115] It is understandable that the light collection diameter refers to the diameter of the light (fluorescence 8 or chemiluminescence) incident on the zoom light collection device 5, which can enter the detection device 4 within a certain diameter range, with the central axis of the zoom light collection device 5 as the center.

[0116] For example, as shown in Figure 11a, at the incident end of the zoom light receiving device 5, light within a diameter range of D1 can enter the zoom light receiving device 5; as shown in Figure 11b, light within a diameter range of D2 can enter the zoom light receiving device 5; as shown in Figure 11c, light within a diameter range of D3 can enter the zoom light receiving device 5, wherein D1 < D2 < D3.

[0117] As shown in Figures 10 and 11, exemplarily, the zoomable light-collecting device 5 may include a first housing 51, multiple lenses 52, and a drive unit 53. The multiple lenses 52 are disposed within the first housing 51 and spaced apart along the transmission directions of fluorescence 8 and chemiluminescence. The number of drive units 53 is at least one, and the drive unit 53 is connected to the lenses 52 to adjust the spacing between at least two adjacent lenses 52. The multiple lenses 52 can focus fluorescence 8 and chemiluminescence onto the detection device 4. Optionally, the number of drive units 53 can be set as needed, as long as the focusing of fluorescence 8 and chemiluminescence onto the detection device 4 can be achieved by adjusting the spacing between the lenses 52. The drive unit 53 may be a linear motor.

[0118] As shown in Figures 11a-11c, by way of example, the plurality of lenses 52 may include at least one lens group 521. The lens group 521 includes a first convex lens 524, a first concave lens 525 and a second convex lens 526 arranged in sequence. The first convex lens 524 is disposed near the detection device 4 and the second convex lens 526 is disposed near the function switching device 3. The distance between the first concave lens 525 and the first convex lens 524 and the second convex lens 526 is adjustable. Specifically, the driving member is connected to the first concave lens 525 to adjust the position of the first concave lens 525. As shown in Figures 11a-11c, moving the first concave lens 525 toward the side where the first concave lens 525 is located, or in other words, moving the first concave lens 525 toward the side where the detection device 4 is located, can increase the light-gathering diameter of the zoom light-gathering device 5. Conversely, moving the first concave lens 525 toward the side where the second convex lens 526 is located, or in other words, moving the first concave lens 525 toward the side where the function switching device 3 is located, can decrease the light-gathering diameter of the zoom light-gathering device 5. That is, the first concave lens 525 gradually moves closer to the first convex lens 524, making D1 < D2 < D3.

[0119] The more lens groups 521 there are, the larger the adjustable range of the light-gathering diameter. For example, when the range of the light-gathering diameter that can be adjusted by one lens group 521 is too small, two or more lens groups 521 can be set.

[0120] In two adjacent lens groups 521, the second convex lens 526 of one lens group 521 serves as the first convex lens 524 of the other lens group 521. The first concave lens 525 in each lens group 521 is connected to the drive unit 53 to synchronously adjust its position. For example, one drive unit 53 simultaneously drives multiple first concave lenses 525 to move, achieving synchronous movement of the multiple first concave lenses 525. By using at least two lens groups 521, the adjustment range of the light-gathering diameter of the zoom light-gathering device 5 is increased, further improving the detection accuracy of the zoom light-gathering device 5.

[0121] For example, as shown in Figure 12, the zoom light-gathering device 5 is provided with two sets of lens groups 521. The lens group 521 located on the upper side of Figure 12 includes a first convex lens 524, a first concave lens 525, and a second convex lens 526 arranged in sequence. The lens group 521 located on the lower side of Figure 12 also includes a first convex lens 524, a first concave lens 525, and a second convex lens 526 arranged in sequence. Furthermore, the second convex lens 526 of the upper lens group 521 and the first convex lens 524 of the lower lens group 521 are the same convex lens. That is, the two adjacent lens groups 521 share a single convex lens, thereby reducing the number of parts in the zoom light-gathering device 5 and lowering its cost. Both sets of first concave lenses 525 are connected to the output end of the drive unit 53 and are driven synchronously by the drive unit 53.

[0122] The number and arrangement of the multiple lenses 52 are not limited to this, as long as the light-gathering diameter of the zoom light-gathering device 5 can be changed.

[0123] Optionally, the dual optical detection device may also include a controller electrically connected to the function switching device 3, thereby allowing the user to select different optical channels 321 as needed. The zoom receiving device 5 and the detection device 4 may also be communicatively connected to the controller, allowing the controller to adjust the receiving diameter of the zoom receiving device 5 based on the type of luminescent liquid, the type of fluorescent substance, or the light intensity detected by the detector 41. The user may also input the wavelength of excitation light 7, fluorescence 8, or chemiluminescence into the controller, which can then switch the optical channel 321 of the function switching device 3 according to the wavelength of excitation light 7, fluorescence 8, or chemiluminescence.

[0124] For example, in the actual detection process, the controller can use an algorithm to control the linear motor connected to the lens 52 to make fine adjustments based on the type of luminescent liquid, the type of fluorescent substance, or the intensity of light, so as to achieve fully automatic zooming and the purpose of focusing light on the detector 41 in different diameter ranges.

[0125] The dual-optical detection device may further include a second driving mechanism connected to the support 1. This second driving mechanism drives the detection box 10 to move in three-dimensional space. On one hand, the detection box 10 may include multiple holes, each containing an independent reaction liquid. The second driving mechanism drives the detection box 10 to move horizontally, allowing for individual detection of the reaction liquid within each hole. On the other hand, when the liquid level of the reaction liquid within the holes changes, the second driving mechanism can also drive the detection box 10 to move vertically, ensuring that the distance between the liquid level of each reaction liquid and the apochromatic focusing device 6 remains consistent. This, in turn, ensures that the position of the excitation light 7 entering the reaction liquid remains consistent, guaranteeing the accuracy of the detection results. The second driving mechanism can be any driving structure that enables three-dimensional movement, such as a robotic arm or a three-dimensional movement driving structure formed by sequentially connecting three linear driving components.

[0126] Optionally, the dual optical detection device may also include a liquid level detection element, which can be connected to the controller. The liquid level detection element can be any structure capable of detecting liquid level, such as an image acquisition element.

[0127] The liquid level detection device and the second drive mechanism can both be electrically connected to the controller, so that the controller controls the second drive mechanism to raise or lower the height of the detection box 10 according to the detection result of the liquid level detection device.

[0128] Regarding the controller: In this embodiment, the controller can be a centralized or distributed controller. For example, the controller can be a single microcontroller or a combination of multiple distributed microcontrollers. The microcontroller can run a control program to control the above-mentioned components to achieve their functions.

[0129] As shown in Figure 5, the dual-optical detection device can detect exosome-related indicators using a combination of chemiluminescence and fluorescence optical detection methods. When the operator requires fluorescence detection, the device enters fluorescence detection mode, placing a sample tube containing the reaction solution in the corresponding hole of the detection box 10. The second drive mechanism of the dual-optical detection device moves the detection box 10 to the corresponding detection position. The reaction solution is aligned with the focal point of the apochromatic focusing device 6. At this time, the excitation light 7 emitted from the light source 21 is redirected 90° downwards through the incident fiber 22 and the dichroic mirror 3212 and enters the sample tube. The reaction solution generates an excitation reaction and emits reaction light (i.e., fluorescence 8) upwards. The reaction light is transmitted to the receiving fiber 42 and enters the detector 41. After processing, the relevant indicator values ​​are obtained. When the testing personnel have a need for chemiluminescence detection, the system enters the chemiluminescence detection state. The sample tube containing the reaction solution is placed in the corresponding hole of the detection box 10. The second drive mechanism of the dual optical detection device moves the detection box 10 to the corresponding detection position. The reaction solution focuses on the apochromatic focusing device 6. At this time, the apochromatic focusing device 6 detects the light signal. The reaction light is transmitted to the receiving optical fiber 42 and enters the detector 41. After processing, the relevant index values ​​are obtained. The number and method of the test samples can be freely selected by the user, which can realize one-time loading and multiple tests.

[0130] Specifically, after absorbing excitation light 7, the reaction solution generates emitted light, namely fluorescence 8. This emitted light passes through an apochromatic focusing device 6 and a channel switching device to reach a zoom-collecting device 5. The zoom-collecting device 5 amplifies and focuses the self-emitting light into the receiving optical fiber 42. Finally, the fluorescence 8 signal is transmitted to the detector 41 through the optical fiber, thereby reflecting the target concentration in the exosome reaction solution.

[0131] When the detection system is set to chemiluminescence detection mode (excitation light 7 is removed in Figure 5, and fluorescence 8 is replaced by chemiluminescence), the function switching device 3 automatically switches to the optical channel 321 corresponding to chemiluminescence detection according to the user's instruction. This optical channel 321 may or may not include a specific dichroic mirror 3212 and filter 3211, or it may include one of the dichroic mirror 3212 and filter 3211, as long as it can transmit chemiluminescence of a specific wavelength. After receiving the chemiluminescence detection instruction, the dual optical detection device checks whether the apochromatic focusing device 6 is compatible with the surface of the reaction liquid, and then adjusts the height of the detection box 10 so that the chemiluminescence can be focused onto the optical channel 321 after passing through the apochromatic focusing device 6. After being focused by the apochromatic focusing device 6, the chemiluminescence light is transmitted through the optical channel 321 of the function switching device 3 to the zoom light receiving device 5. After receiving the light energy signal, the zoom light receiving device 5 adjusts the displacement of the first concave lens 525 to amplify and focus the light signal into the receiving optical fiber 42. Finally, the light signal from the reaction liquid is transmitted to the detector 41 through the receiving optical fiber 42.

[0132] In this embodiment, the zoom light-collecting device 5, the function switching device 3, and the apochromatic focusing device 6 form a spatial optical path detection system. Together with the light source 21 and the detector 41, it realizes the detection of two optical signals, chemiluminescence and fluorescence 8, ensuring that the detection positions of the sample optical signals collected by different optical channels 321 are consistent. The zoom light-collecting device 5 can automatically change the light-collecting diameter to ensure the maximum area of ​​light collection range.

[0133] The dual-optical detection device can perform multi-sample dual-mode detection, enabling the detection of chemiluminescence and fluorescence signals separately with a single sample loading. The dual-optical detection device is highly integrated, realizing two optical detection functions in one system space. The dual-optical detection device has a high degree of automation and good compatibility with samples.

[0134] This embodiment also provides a detection method, which is performed by the aforementioned dual-optical detection device. The detection method may include the following steps:

[0135] S1. According to the detection method required by the reaction solution in the detection box 10, switch the function switching device 3 to the corresponding fluorescence detection state or chemiluminescence detection state.

[0136] S2, the zoom light-collecting device 5 changes its light-collecting diameter according to the intensity of the detected fluorescence 8 or chemiluminescence; or the zoom light-collecting device 5 changes its light-collecting diameter according to the type of luminescent liquid; or the zoom light-collecting device 5 changes its light-collecting diameter according to the type of fluorescent substance.

[0137] S3. Test the reaction solution.

[0138] The detection method can perform dual-mode detection, detecting chemiluminescence and fluorescence signals separately. The zoom-collecting device 5 adjusts its collecting diameter according to the intensity of the detected fluorescence or chemiluminescence, the type of luminescent liquid, or the type of fluorescent substance. This ensures that the detection device 4 can receive an appropriate light signal regardless of whether the light signal is weak or too strong, thereby improving the accuracy of the detection results.

[0139] Optionally, before step S1, the method may further include: placing the detection box 10 on the bracket 1.

[0140] Although this application has been described in detail above with general descriptions, specific embodiments, and experiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of this application fall within the scope of protection claimed in this application.

Claims

1. A dual optical detection device, characterized in that, include: A light source device (2) is used to emit excitation light (7); The function switching device (3) can switch between fluorescence detection state and chemiluminescence detection state; The detection device (4) is used to detect fluorescence (8) and chemiluminescence in the reaction solution; The zoom light-collecting device (5) is located between the function switching device (3) and the detection device (4), and the zoom light-collecting device (5) can change its light-collecting diameter.

2. The dual optical detection device according to claim 1, characterized in that, The zoom light-collecting device (5) can change its light-collecting diameter according to the intensity of the fluorescence (8) or the chemiluminescence; or The zoom light-collecting device (5) adjusts its light-collecting diameter according to the type of luminescent liquid; or The zoom light-collecting device (5) adjusts its light-collecting diameter according to the type of fluorescent material; or The zoom-focusing light-collecting device (5) can focus the fluorescence (8) or the chemiluminescence onto the detection device (4); or The zoom light-receiving device (5) is connected to the function switching device (3).

3. The dual optical detection device according to claim 2, characterized in that, When the intensity of the fluorescence (8) or the chemiluminescence exceeds a minimum preset value, the zoom light-collecting device (5) increases its light-collecting diameter; and / or when the intensity of the fluorescence (8) or the chemiluminescence exceeds a maximum preset value, the zoom light-collecting device (5) decreases its light-collecting diameter; or If the luminescent liquid has a high efficiency in generating the chemiluminescence, the zoom light-collecting device (5) reduces its light-collecting diameter; if the luminescent liquid has a low efficiency in generating the chemiluminescence, the zoom light-collecting device (5) increases its light-collecting diameter; or If the fluorescence quantum yield of the fluorescent material is high, the zoom light-collecting device (5) reduces its light-collecting diameter; If the fluorescence quantum yield of the fluorescent material is low, then the zoom receiver The light receiving device (5) increases its light receiving diameter.

4. The dual optical detection device according to any one of claims 2-3, characterized in that, The zoom light-collecting device (5) includes a first housing (51), a plurality of lenses (52) and a driving member. The plurality of lenses (52) are disposed in the first housing (51) and spaced apart along the transmission direction of the fluorescence (8) and the chemiluminescence. The number of driving members is at least one. The driving member is connected to the lens (52) to adjust the spacing between at least two adjacent lenses (52). The plurality of lenses (52) can focus the fluorescence (8) and the chemiluminescence onto the detection device (4).

5. The dual optical detection device according to claim 4, characterized in that, The plurality of lenses (52) includes at least one lens group (521), the lens group (521) including a first convex lens (524), a first concave lens (525) and a second convex lens (526) arranged in sequence, wherein the first convex lens (524) is disposed close to the detection device (4) and the second convex lens (526) is disposed close to the function switching device (3), and the distance between the first concave lens (525) and the first convex lens (524) and the second convex lens (526) is adjustable.

6. The dual optical detection device according to claim 5, characterized in that, At least two sets of the lens groups (521) are provided, wherein in two adjacent sets of the lens groups (521), the second convex lens (526) of one set of the lens groups (521) is the first convex lens (524) of the other set of the lens groups (521).

7. The dual optical detection device according to any one of claims 2-3, characterized in that, The function switching device (3) includes at least two optical channels (321) and a working position; Each of the optical channels (321) can be located at the working position so that at least one of the optical channels (321) can transmit the chemiluminescence to the detection device (4), so that the function switching device (3) is in the chemiluminescence detection state, and so that at least one of the optical channels (321) can transmit the excitation light (7) of a preset wavelength to the reaction solution and transmit the fluorescence (8) to the detection device (4), so that the function switching device (3) is in the fluorescence detection state.

8. The dual optical detection device according to claim 7, characterized in that, At least one of the optical channels (321) includes a filter (3211) and a dichroic mirror (3212). The filter (3211) allows excitation light (7) of a preset wavelength to enter the optical channel (321), and the dichroic mirror (3212) allows fluorescence (8) of a preset wavelength to pass through, so that the fluorescence (8) enters the detection device (4).

9. The dual optical detection device according to claim 8, characterized in that, The function switching device (3) includes a second housing (31) and a mounting bracket (32). The mounting bracket (32) includes at least two optical channels (321). The mounting bracket (32) is movably disposed on the second housing (31) so that each optical channel (321) can be located at the working position. The optical channel (321) located at the working position can transmit the excitation light (7) and the fluorescence (8) of a preset wavelength or can transmit the chemiluminescence.

10. The dual optical detection device according to claim 9, characterized in that, The mounting bracket (32) is rotatably disposed within the second housing (31), and the at least two optical channels (321) are spaced apart along the rotation center of the mounting bracket (32); and / or The second housing (31) includes a first light inlet (311), a first light outlet (312), and a first light outlet (313). The optical channel (321) includes a second light inlet (3213), a second light outlet (3234), and a second light outlet (3235). When the optical channel (321) is in the working position, the first light inlet (311) and the second light inlet (3213) are directly opposite each other. The first light inlet (311) and the second light outlet (3235) are directly opposite each other. The excitation light (7) enters the second housing (31) through the first light inlet (311). The excitation light (7) of the preset wavelength is emitted out of the second housing (31) through the first light inlet / outlet (312). The fluorescence (8) and the chemiluminescence enter the second housing (31) through the first light inlet / outlet (312) and are emitted out of the second housing (31) through the first light outlet (313).

11. The dual optical detection device according to claim 10, characterized in that, The mounting frame (32) includes a frame (322) which is rotatably connected to the second housing (31). The optical channel (321) also includes an optical tube (3236) which is detachably connected to the frame (322). The at least one optical tube (3236) is provided with the filter (3211) and the dichroic mirror (3212).

12. The dual optical detection device according to claim 11, characterized in that, The function switching device (3) further includes a first drive mechanism (33), which is connected to the second housing (31) and the mounting bracket (32) and drives the mounting bracket (32) to rotate.

13. The dual optical detection device according to claim 10, characterized in that, The second light outlet (3235) and the second light inlet / outlet (3234) are located on the same straight line, and the second light inlet (3213) is perpendicular to the second light outlet (3235); The filter (3211) is disposed at the second light inlet (3213), and the dichroic mirror (3212) is disposed at the light channel (321), and is at a 45° angle to both the second light inlet (3213) and the second light outlet (3234).

14. The dual optical detection device according to claim 1, characterized in that, The light source device (2) includes a light source (21) and an incident optical fiber (22), the incident optical fiber (22) being used to transmit the excitation light (7) emitted by the light source (21) to the function switching device (3); and / or The detection device (4) includes a detector (41) and a receiving optical fiber (42). The receiving optical fiber (42) is used to transmit the fluorescence (8) and chemiluminescence output by the function switching device (3) to the detector (41). The detector (41) is used to detect the fluorescence (8) and the chemiluminescence; and / or The dual optical detection device also includes a second driving mechanism, which drives the detection box (10) containing the reaction liquid to move in three-dimensional space.

15. The dual optical detection device according to claim 1, characterized in that, The dual optical detection device also includes an apochromatic focusing device (6), which is located downstream of the function switching device (3). The apochromatic focusing device (6) is used to focus the beams formed by the excitation light (7) of different wavelengths onto the same test site.

16. A detection method, characterized in that, The dual-optical detection device according to any one of claims 1-15 performs the detection method, the detection method comprising the following steps: According to the detection method required by the reaction liquid in the detection box (10), switch the function switching device (3) to the corresponding fluorescence detection state or chemiluminescence detection state; The zoom light-collecting device (5) changes its light-collecting diameter according to the intensity of the detected fluorescence (8) or chemiluminescence; or the zoom light-collecting device (5) changes its light-collecting diameter according to the type of luminescent liquid; or the zoom light-collecting device (5) changes its light-collecting diameter according to the type of fluorescent substance. The reaction solution was tested.

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