Cosmetic compositions and methods of moisturizing skin

Abstract

Disclosed are cosmetic compositions including an aquaporin 3 stimulator, optionally a calcium ion delivery agent, an osmolytic transport stimulator, a lipid mixture, and hyaluronic acid or a salt thereof. Also disclosed are methods of moisturizing skin by applying the cosmetic compositions to the skin.

Description

Cosmetic Compositions and Methods of Moisturizing Skin CROSS REFERENCE TO RELATED APPLICATIONS

[0001] The present application claims priority to and the benefit of U.S. App. No. 63 / 660,827, filed June 17, 2024, the entire contents of which are hereby incorporated by reference in their entirety.BACKGROUND

[0002] Cosmetic compositions can be used to moisturize skin. How well a cosmetic composition moisturizes skin may depend on a variety of factors such as, for example, the specific components of the cosmetic composition, the method of moisturizing the skin with the cosmetic composition, and / or differences between skin types. There is a continuing need for cosmetic compositions with improved skin moisturizing properties. The inventors unexpectedly discovered that the cosmetic compositions disclosed herein, which contain an aquaporin 3 stimulator, a calcium ion delivery agent, an osmolytic transport stimulator, a lipid mixture, and hyaluronic acid or a salt thereof provide an enhanced moisturizing effect when applied to skin.SUMMARY

[0003] Disclosed are compositions that include an aquaporin 3 stimulator, a calcium ion delivery agent, an osmolytic transport stimulator, a lipid mixture, and hyaluronic acid or a salt thereof. Disclosed are compositions that include an aquaporin 3 stimulator, a calcium ion delivery agent, an osmolytic transport stimulator, a lipid mixture, hyaluronic acid or a salt thereof..

[0004] For example, the composition may comprise an aquaporin 3 (AQP3) stimulator, optionally a calcium ion delivery agent, an osmolytic transport stimulator, a lipid mixture, and hyaluronic acid or a salt thereof. The calcium ion delivery agent may comprise hydroxyapatite. In various implementations, the AQP3 stimulator is selected from the group consisting of haematococcus pluvialis extract, isosorbide dicaprylate, or a combination thereof. In certain aspects, the AQP3 stimulator is haematococcus pluvialis extract and the composition (e.g., cosmetic, topical) comprises from 0.01 weight percent to 1 weight percent haematococcus pluvialis extract by total weight of the composition (e.g., cosmetic, topical), and / or isosorbide dicaprylate in an amount from 0.5 weight percent to 2.5 weight percent. In some embodiments, the the lipid mixture comprises two or morelipids chosen from squalane, euphorbia cerifera wax, butyrospennum parkii buter, beta-sitosterol, oleic acid, glyceryl behenate, polyglycervl-4 isostearate, lecithin, a ceramide, tocopherol, ethylhexyl palmitate, stearic acid, and trihydroxystearin. In various implementations, the lipid mixture comprises lecithin, ethylhexyl palmitate, and trihydroxy stearin. In some embodiments, tire lipid mixture comprises squalane, euphorbia cerifera wax, butyrospennum parkii buter, beta-sitosterol, oleic acid, glyceryl behenate, polyglyceryl-4 isostearate, lecithin, a ceramide, tocopherol, ethylhexyl palmitate, stearic acid, and trihydroxystearin. In certain embodiments, the the osmolytic transport stimulator is hibiscus sabdariffa fruit extract, trehalose, or a combination thereof. For example, the composition may comprise from 0.001 weight percent to 0.03 weight percent hibiscus sabdariffa fruit extract by total weight of the composition (e.g.. cosmetic, topical) and / or 0.001 weight percent to 0.1 weight percent trehalose by weight of the composition. In certain instances, the composition (e g., cosmetic, topical) comprises from 0.001 weight percent to 0.1 weight percent hyaluronic acid or a salt thereof by total weight of the composition (e.g.. cosmetic, topical). In some embodiments, the composition further comprises isododecane. In certain aspects, the composition further comprises cetyl alcohol. In some embodiments, the composition further comprises glyceryl stearate citrate, ceteaiyl alcohol, and glyceryl caprylate. In some embodiments, the composition further comprises dimethicone, erythritol, polysilicone-11, bis-digly ceryl polyacyladipate-2, phenoxyethanol, ethylhexylglycerin, collagen amino acids, or a combination thereof. In various aspects, the composition is a cream or a scrum.

[0005] In various implementations, the composition comprises at least three components selected from 1,2-Hexanediol. Caprylhydroxamic Acid. Erythritol, Ethylhexyl Palmitate. Glucomannan. Glycerin, Glyceryl Oleate Citrate, Haematococcus Pluvialis Extract, Hibiscus Sabdariffa Fruit Extract, Hydrolyzed Haematococcus Pluvialis Extract, Hydroxyapatite, PPG-24-Glyccrcth-24, Propanediol. Sodium Hyaluronate, Sodium Polyacryloyldimethyl Taurate, Trihydroxystearin, Trisodium Ethylenediamine Disuccinate, and Water. In various implementations, the composition comprises at least three components selected from 1,2-Hexanediol, Beta-Sitosterol, Bis-Diglyceryl Polyacyladipate-2, Butylene Glycol, Butyrospermum Parkii (Shea) Buter, Carbomer, Ceramide NG, Ceramide NP, Cetearyl Alcohol, Cet l Alcohol, Dimethicone, Disodium EDTA. Erythritol.Ethylhexy l Palmitate, Ethy lhexylglycerin, Euphorbia Cerifera (Candelilla) Wax, Glucomannan, Glycerin, Glyceryl Behenate, Glyceryl Caprylate, Glyceryl Stearate, Glyceryl Stearate Citrate, Haematococcus Pluvialis Extract, Hibiscus Sabdariffa Fruit Extract, Hydrolyzed Haematococcus Pluvialis Extract, Hydroxyapatite, Isododecane, Isosorbide Dicaprylate, Lecithin, Oleic Acid, Phenoxyethanol, Phenyl Trimethicone, Polyglyceryl-4 Isostearate, Polysilicone-11, Propanediol, Sodium Acrylates Copolymer, Sodium Hyaluronate, Sodium Hydroxide, Squalane, Stearic Acid, Tocopherol. Tocopheryl Acetate. Trihydroxystearin. Water, and Xanthan Gum. In various implementations, the composition comprises at least three components selected from 1,2-Hexanediol, Allantoin, Butyrospennum Parkii (Shea) Butter, Caprylyl Glycol. Ceramide AP. Ceramide EOP, Ceramide NP. Cetearyl Alcohol, Cetearyl Glucoside, Cetyl Alcohol, Cholesterol, Citric Acid, Dihydroxy Methylchromone. Dimethicone, Ethyl Linoleate, Ethyl Linolenate, Ethyl Oleate, Ethyl Palmitate, Ethyl Stearate, Fructose, Glucose, Glycerin, Glycery l Stearate, Glyceryl Stearate SE, Glycosphingolipids, Helianthus Annuus (Sunflower) Seed Wax, Hydrogenated Lecithin, Hydrogenated Polydecene. Hydroxyapatite. Isopropyl Palmitate, Isosorbide Dicaprylate. Jojoba Oil / Macadainia Seed Oil Esters, Maltose, Pcntacrvtliritvl Tetra-di-t-butyl Hydroxyhydrocinnamate, Pentylene Glycol, Phenylpropanol, Phytosphingosine, Phytosterols. Phytosteryl Macadamiate, Polysorbate 20. Propanediol, Retinol, Sodium Chloride, Sodium Hyaluronate, Sodium Hydroxide, Sodium Lactate, Sodium PCA, Squalene, Stearic Acid, Tetraacetylphytosphingosine, Tocopherol, Trehalose, Urea, Water, and Xanthan Guin. In various implementations, the composition comprises at least three components selected from 1,2-Hexanediol, Caprylhydroxamic Acid, Cetearyl Olivate. Cetyl Alcohol, Dimer Dilinoleyl Dimer Dilinoleate, Dimethicone, Erythritol, Glycerin, Haematococcus Pluvialis Extract, Hibiscus Sabdariffa Fruit Extract, Hydrolyzed Haematococcus Pluvialis Extract, Isopropyl Palmitate, Palmitic Acid, Polyacrylate Crosspolymer-6, Propanediol, Sodium Hyaluronate. Sorbitan Olivate, Stearic Acid, and Water. In various implementations, more than 90% or more than 95% or more than 99% or more than 99.5% of the composition by weight comprises three or more of the indicated components.

[0006] Also disclosed are methods of moisturizing skin, comprising applying a disclosed composition to the skin.DETAILED DESCRIPTION

[0007] Unless otherwise indicated, all parts and percentages are by weight. As used herein, the term “about” refers to plus or minus 10% of the indicated value. Unless otherw ise stated or made clear by context, weight percentages are provided based on the total amount of the composition in which they arc described. As used herein, the singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise. The word “or” is the inclusive or and is used synonymously with “and / or” such that “A or B” includes embodiments having only A, embodiments having only B, and embodiments having both A and B.

[0008] Compositions

[0009] Disclosed are compositions such as cosmetic compositions and / or topical compositions that may include an aquaporin 3 stimulator, an optional calcium ion delivery agent, an osmolytic transport stimulator, a lipid mixture, and hyaluronic acid or a salt thereof. In contrast to existing cosmetic compositions that passively trap or collect moisture so that skin feels hydrated, the disclosed composition provide an active mechanism to functionally provide moisture from within cells. The compositions can also transport moisture, promote water homeostasis and improve epidermal barrier function via water channeling and tire use of osmolytes to provide moisturization to the skin surface.

[0010] Aquaporin 3 stimulators

[0011] Aquaporin 3 (AQP3) stimulators can increase the amount AQP3 expression or upregulate AQP3 expression when applied to the skin. Haematococcus pluvialis extract and isosorbide dicaprylate are examples of AQP3 stimulators. Haematococcus pluvialis extract can help stimulate collagen product and / or improve skin properties. In some embodiments, the cosmetic composition comprises from 0.01 w eight percent to 1 weight AQP3 stimulators by total weight of the cosmetic composition. In some embodiments, the cosmetic composition comprises from 0.01 weight percent to 1 weight percent haematococcus pluvialis extract by total weight of the cosmetic composition. For example, in some embodiments, the cosmetic composition includes from 0.01 w eight percent to 0.2 w eight percent haematococcus pluvialis extract by total weight of the cosmetic composition. In someembodiments, the cosmetic composition includes from about 0.04 weight percent to about 0.15 weight percent haematococcus pluvialis extract by total weight of the cosmetic composition.

[0012] Isosorbide dicaprylate improves hydration and epidermal barrier function in human skin by upregulating expression of AQP3. In some embodiments, the cosmetic composition includes from 0.1 weight percent to 5 weight percent isosorbidc dicaprylatc by total weight of the cosmetic composition. For example, in some embodiments, the cosmetic composition includes from 1 weight percent to 3 weight percent isosorbide dicaprylate by total weight of the cosmetic composition. In some embodiments, the cosmetic composition includes from about 2 weight percent isosorbide dicaprylate by total weight of the cosmetic composition.

[0013] In some embodiments, the AQP3 stimulator includes a combination of isosorbide dicaprylate and haematococcus pluvialis extract. For example, in some embodiments the cosmetic composition contains from 0.01 weight percent to 1 weight percent haematococcus pluvialis extract and from 0.1 weight percent to 5 weight percent isosorbide dicaprylate by total weight of the cosmetic composition. In some embodiments, the cosmetic composition includes from 0.01 weight percent to 0.2 weight percent haematococcus pluvialis extract and from 1 weight percent to 3 weight percent isosorbide dicaprylate by total weight of the cosmetic composition. In some embodiments, the cosmetic composition includes from 0.04 weight percent to 0.8 weight percent haematococcus pluvialis extract and about 2 3 weight percent isosorbide dicaprylate by total weight of the cosmetic composition.

[0014] In some embodiments, the AQP3 stimulator, for example, haematococcus pluvialis extract is added in combination with one or more collagen precursors, for example, collagen amino acids, collagen peptides, or hydrolyzed haematococcus pluvialis extract. In some embodiments, the cosmetic composition contains from 0.1 weight percent to 5 weight percent collagen precursors by total weight of the cosmetic composition.

[0015] Calcium ion delivery agents

[0016] Calcium ion delivery agents can help balance the electrolyte content of the skin. In some embodiments, the calcium delivery agent releases an effective amount of calcium ions over a period of time such as, for example, from 30 minutes to 72 hours (or any subrange contained therein). Suchcalcium delivery agents may improve the moisturizing effect of the cosmetic composition by providing a trans epidermal calcium gradient. In some embodiments, the calcium ion delivery agent includes hydroxyapatite. In some embodiments, the cosmetic composition includes from 0.01 weight percent to 1 weight percent hydroxyapatite by total weight of the cosmetic composition. For example, in some embodiments, the cosmetic composition includes from 0.05 weight percent to 0.5 weight percent hydroxyapatite by total weight of the cosmetic composition. In some embodiments, the cosmetic composition includes from about 0.1 weight percent to about 0.3 weight percent hydroxyapatite by total weight of the cosmetic composition.

[0017] Osmolytic transport stimulators

[0018] Osmolytic transport stimulators can stimulate organic osmolytes cellular intake when applied to the skin. Hibiscus sabdariffa fruit extract and betaine are examples of osmolytic transport stimulators. Hibiscus sabdariffa fruit extract can improve skin moisture by balancing osmolyte and / or osmolyte transport properties within the skin. For example, hibiscus sabdariffa fruit extract may contain osmolytes, such as betaine, that may improve skin moisture by restoring water content and / or reducing water loss from the skin. In some embodiments, the cosmetic composition includes from 0.001 weight percent to 0.03 weight percent of osmolytic transport stimulators by total weight of the cosmetic composition. In some embodiments, the cosmetic composition includes from 0.001 weight percent to 0.03 weight percent hibiscus sabdariffa fruit extract by total weight of the cosmetic composition. In some embodiments, the cosmetic composition includes from 0.001 weight percent to 0.03 weight percent of betaine by total weight of the cosmetic composition.

[0019] Lipids

[0020] Lipids can help improve skin moisture by, for example, reducing water loss from the skin and / or improving cell membrane physiology. Different lipids can have different effects on the skin and mixtures of different lipids may provide synergistic results. In some embodiments, the lipid mixture includes two or more lipids chosen from squalane, euphorbia cerifera wax, butyrospermum parkii butter, beta-sitosterol, oleic acid, glyceryl behenate, polyglyceryl-4 isostearate, lecithin, a ceramide, tocopherol, ethylhexyl palmitate, stearic acid, and trihydroxystearin.

[0021] In some embodiments, the lipid mixture comprises three or more lipids chosen from squalane, euphorbia cerifera wax, butyrospennum parkii butter, beta-sitosterol, oleic acid, glyceryl behenate, polyglyceryl-4 isostearate, lecithin, a sphingolipid such as a ceramide, cholesterol (esters), phospholipids, tocopherol, ethylhexyl palmitate, stearic acid, and trihydroxystearin.

[0022] In some embodiments, the lipid mixture also includes lecithin, ethylhexyl palmitate, and trihydroxy stearin.

[0023] In some embodiments, the lipid mixture also includes squalane, euphorbia cerifera wax, butyrospermum parkii butter, beta-sitosterol, oleic acid, glyceryl behenate, polyglyceryl-4 isostearate, lecithin, a ceramide, tocopherol, ethylhexyl pahnitate, stearic acid, and trihydroxystearin.

[0024] In some embodiments, the cosmetic composition includes from 0.01 weight percent to 0.1 weight percent lecithin, from 0.1 weight percent to 5 weight percent ethylhexyl palmitate, and from 0.001 weight percent to 0.1 weight percent trihydroxy stearin by total weight of the cosmetic composition.

[0025] In some embodiments, the cosmetic composition includes from 0.1 weight percent to 0.5 weight percent squalane, from 0.01 weight percent to 0.1 weight percent euphorbia cerifera wax, from 0.01 weight percent to 2 weight percent butyrospermum parkii butter, from 0.01 weight percent to 0.1 weight percent beta-sitosterol, from 0.01 weight percent to 0.1 weight percent oleic acid, from 0.01 weight percent to 0.1 weight percent glyceryl behenate, from 0.01 weight percent to 0.05 weight percent polyglyceryl-4 isostearate, from 0.01 weight percent to 0.1 weight percent lecithin, from 0.1 weight percent to 5 weight percent ethylhexyl palmitate, from 0.1 weight percent to 3 weight percent stearic acid, and from 0.001 weight percent to 0.1 weight percent trihydroxystearin by total weight of the cosmetic composition.

[0026] Hyaluronic acid or a sali thereof

[0027] The hyaluronic acid or a salt thereof may be a free hyaluronic acid, a crosslinked hyaluronic acid derivative, and / or salts of the free acid or derivative thereof. Acceptable salts include, for example, alkali metal salts such as potassium hyaluronate and sodium hyaluronate. For example, in some embodiments, the hyaluronic acid or a salt thereof is sodium hyaluronate.

[0028] The hyaluronic acid or a salt thereof can have any particular molecular weight, can be linear, crosslinked, or crosslinked with other components. For example, hyaluronic acid or a salt thereof can a molecular weight of from about 1 kDa to about 20,000 kDa. In some embodiments, the hyaluronic acid or a salt thereof has a molecular weight of about 1 kDa to about 20,000 kDa, for example, about 1 kDa to about 10,000, about 1 kDa to about 5,000 kDa, about 1 kDa to about 1,000 kDa, about 1 kDa to about 100 kDa, about 25 kDa to about 100 kDa, about 25 kDa to about 50 kDa, or about 50 kDa or less. In some embodiments, the hyaluronic acid or a salt thereof has a molecular weight of about 10 kDa to about 40 kDa, for example, about 1 kDa to about 20 kDa, or about 3 kDa to about 10 kDa. In some embodiments, the hyaluronic acid or a salt thereof has a molecular weight of about 500 kDa to about 2000 kDa, for example about 600 kDa to about 1000 kDa. In some embodiments, the hyaluronic acid or a salt thereof has a molecular weight of 10 kDa to 40 kDa. Hyaluronic acids or salts thereof with different molecular weights can be obtained by, for example, selective production methods that generate particular molecular weight ranges or by controlled hydrolysis of higher molecular weight hyaluronic acids or salts thereof. In some embodiments, the cosmetic composition includes from 0.001 weight percent to 0.1 hyaluronic acid or a salt thereof by total weight of the cosmetic composition. For example, in some embodiments, the cosmetic composition includes from 0.001 weight percent to 0.1 sodium hyaluronate by total weight of the cosmetic composition.

[0029] In exemplary embodiments, at least a portion of the hyaluronic acid or a salt thereof is crosslinked with konjac glucomannan in the form of dehydrated Ultra Filling Spheres™ (from BASF), as disclosed in US2015 / 0283055A1, the entire contents of which are incorporated by reference. Ultra Filling Spheres™ have been shown to penetrate the epidermis, absorbing water to smooth fine lines and deep wrinkles, improve elasticity, and provide long term hydration.

[0030] Ultra Filling Spheres™ contain hyaluronic acid or a salt thereof as a combination of about 97.5 weight percent HA and about 2.5 weight percent glucomannan, for example konjac glucomannan, dispersed at a concentration of about 0.2 weight percent in ethylhexyl palmitate (about 97.8 weight percent of the total Ultra Filling Spheres™ formulation) and trihydroxy stearin (about 2.0weight percent of the total Ultra Filling Spheres™ formulation). The sodium hyaluronate present in the Ultra Filling Spheres™ typically has a molecular weight of 10 kDa to 40 kDa.

[0031] In some embodiments of the invention, the cosmetic composition contains about 0.1 weight percent to about 5.0 weight percent Ultra Filling Spheres™. In some embodiments of the invention, the cosmetic compositions contain about 0.3 weight percent, about 0.5 weight percent, about 1.0 weight percent, about 2.0 weight percent, about 3.0 weight percent, about 4.0 weight percent, about 5.0 weight percent, about 6.0 weight percent, about 7.0 weight percent, about 8.0 weight percent, about 9.0 weight percent, or about 10.0 weight percent Ultra Filling Spheres™. In an exemplary embodiment, the cosmetic composition contains about 0.5 weight percent Ultra Filling Spheres™. In an exemplary embodiment, the cosmetic composition contains about 2.0 weight percent Ultra Filling Spheres™.

[0032] While one can use Ultra Filling Spheres™, other microspheres containing hyaluronic acid or a salt thereof are suitable for use in the methods and compositions according to the invention. Alternative HA microspheres include, but are not limited to Hyaluronic Filling Spheres® also supplied by BASF, which use a hygroscopic material to make a sphere, but do not include konjac glucomannan. Other Hyaluronic Filling Spheres technology is based on the use of cross-linked spheres.

[0033] In some embodiments, the amount of hyaluronic acid or a salt thereof in the cosmetic composition contained in the Ultra Filling Spheres™ may range from about 0.5% to about 50% of the total amount of Ultra Filling Spheres™. In some embodiments, the amount of hyaluronic acid or a salt thereof in the cosmetic composition contained in the Ultra Filling Spheres™ may range from about 1.5% to about 2% of the total amount of hyaluronic acid or a salt thereof. In some embodiments, the amount of hyaluronic acid or a salt thereof in the cosmetic composition contained in the Ultra Filling Spheres™ may range from about 25% to about 35% of the total amount of hyaluronic acid or a salt thereof, for example about 30% of the total amount of hyaluronic acid or a salt thereof.

[0034] Additional components

[0035] The cosmetic composition comprising an AQP3 stimulator, optionally a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof can include additional components. The additional components can be active components that improve one or more skin property when applied to the skin alone or in combination in the cosmetic composition. The additional components can have synergistic effects when combined with an AQP3 stimulator, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof. The additional components can improve physical properties of the cosmetic composition and / or other properties of the cosmetic composition such as, for example, visual appeal, smell, etc.. Such additional components include, for example, emulsifiers (e.g., nonionic, cationic, anionic, and / or polymeric emulsifiers), rheology modifiers, humectants, surfactants (e.g., non-ionic. cationic, and / or anionic surfactants), emollients. pH modifiers and / or buffers, antimicrobial agents, preservatives, antioxidants, film forming agents, colorants, fragrances, essential oils, etc.

[0036] In some embodiments, the cosmetic composition also includes isododecane. For example, in some embodiments, the cosmetic composition includes from 1 weight percent to 10 weight percent isododecane by total weight of the cosmetic composition.

[0037] In some embodiments, the cosmetic composition also includes one or more fatty acid esters. In some embodiments, one or more fatty acid esters include shea butter esters, e.g., shea butter ethyl esters, jojoba esters, or a combination thereof. In some embodiments, the cosmetic composition includes from 1 weight percent to 10 weight percent fatty acid esters by total weight of the cosmetic composition.

[0038] In some embodiments, the cosmetic composition also includes butylene glycol. For example, in some embodiments, the cosmetic composition includes from 1 weight percent to 10 weight percent butylene glycol by total weight of the cosmetic composition.

[0039] In some embodiments, the cosmetic composition also includes phenyl trimethicone. For example, in some embodiments, the cosmetic composition includes from 1 weight percent to 10 weight percent phenyl trimethicone by total weight of the cosmetic composition.

[0040] In some embodiments, the cosmetic composition also includes cetyl alcohol. For example, in some embodiments, the cosmetic composition includes from 1 weight percent to 10 weight percent cetyl alcohol by total weight of the cosmetic composition.

[0041] In some embodiments, the cosmetic composition also includes glyceryl stearate citrate, cetearyl alcohol, and glyceryl caprylate. For example, in some embodiments, the cosmetic composition includes from 0.1 weight percent to 5 weight percent glycery l stearate citrate, from 0.1 weight percent to 3 weight percent cetearyl alcohol, and from 0.1 weight percent to 3 weight percent glyceryl caprylate by total weight of the cosmetic composition.

[0042] In some embodiments, the cosmetic composition also includes glycery l stearate. For example, in some embodiments, the cosmetic composition includes from 0.1 weight percent to 5 weight percent glyceryl stearate by total weight of the cosmetic composition.

[0043] In some embodiments, the cosmetic composition also includes dimethicone. For example, in some embodiments, the cosmetic composition includes from 0.1 weight percent to 5 weight percent dimethicone by total weight of the cosmetic composition.

[0044] In some embodiments, the cosmetic composition also includes erythritol. For example, in some embodiments, the cosmetic composition includes from 0.01 weight percent to 0.5 weight percent ery thritol by total weight of the cosmetic composition.

[0045] In some embodiments, the cosmetic composition also includes polysilicone-11. For example, in some embodiments, the cosmetic composition includes from 0.01 weight percent to 0.5 weight percent polysilicone-11 by total weight of the cosmetic composition.

[0046] In some embodiments, the cosmetic composition also includes bis-diglycery 1 polyacyladipate- 2. For example, in some embodiments, the cosmetic composition includes from 0.1 weight percent to 5 weight percent bis-diglyceryl polyacyladipate-2 by total weight of the cosmetic composition.

[0047] In some embodiments, the cosmetic composition also includes phenoxyethanol and ethylhexylglycerin. For example, in some embodiments, the cosmetic composition includes from 0.1 weight percent to 2 weight percent phenoxyethanol and from 0.01 weight percent to 0.2 weight percent ethylhexylglycerin by total weight of the cosmetic composition.

[0048] In some embodiments, the cosmetic composition also includes collagen amino acids, collagen peptides, and / or collagen precursors. In some embodiments, the cosmetic composition also includes collagen amino acids, collagen peptides, and / or collagen precursors as hydrolyzed haematococcus pluvialis extract. In some embodiments, the cosmetic composition also includes collagen amino acids and / or collagen peptides.

[0049] In some embodiments, the cosmetic composition also includes tocopheryl acetate. For example, in some embodiments, the cosmetic composition includes from 0.01 weight percent to 0.5 weight percent tocopheryl acetate by total weight of the cosmetic composition.

[0050] In some embodiments, the cosmetic composition also includes caprylhydroxamic acid. For example, in some embodiments, the cosmetic composition includes from 0.01 weight percent to 0.5 weight percent caprylhydroxamic acid by total weight of the cosmetic composition.

[0051] In some embodiments, the cosmetic composition also includes PPG-24-Glycereth-24. For example, in some embodiments, the cosmetic composition includes from 1 weight percent to 5 weight percent PPG-24-Glycereth-24 by total weight of the cosmetic composition.

[0052] In some embodiments, the cosmetic composition also includes sodium polyacryloyldimethyl taurate. For example, in some embodiments, the cosmetic composition includes from 0.1 weight percent to 2 weight percent sodium polyacn loyldimethyl taurate by total weight of the cosmetic composition.

[0053] The cosmetic composition can comprise various amounts of water. For example, in some embodiments, the cosmetic composition includes at least 60 weight percent water by total weight of the cosmetic composition. In some embodiments, the cosmetic composition includes at least 85 weight percent water by total weight of the cosmetic composition. In some embodiments, the cosmetic composition includes from 60 weight percent to 90 weight percent water by total weight of the cosmetic composition.

[0054] The cosmetic composition can independently comprise various combinations and amounts of the components disclosed herein. For example, in some embodiments, the cosmetic composition includes water, isododecane, butylene glycol phenyl trimethicone, 1,2-hexanediol, sodiumhyaluronate, cety l alcohol, glyceryl stearate citrate, cetear l alcohol, glycery l caprylate, glycery l stearate, dimethicone, isosorbide dicaprylate, glycerin, erythritol, hibiscus sabdariffa fruit extract, poly silicone- 11. butyrospermum parkii butter, bis-digly ceryl polyacyladipate-2. stearic acid, phenoxyethanol, ethylhexylglycerin, glycerin, propanediol, collagen amino acids, haernatococcus pluvialis extract, squalane, euphorbia cerifera wax, beta-sitosterol, oleic acid, glyceryl behenate, polyglyceryl-4 isostearate, lecithin, ceramide NG, ceramide NP, tocopherol, cthyllicxyl palmitate, trihydroxy' stearin, sodium hyaluronate, glucomannan, sodium hydroxide, sodium acrylates copolymer, carbomer, xanthan gum, hydroxyapatite, tocopheryl acetate, and disodium EDTA.

[0055] In some embodiments, the cosmetic composition consists essentially of water, isododecane, butylene glycol phenyl trimethicone, 1,2-hexanediol. sodium hyaluronate, cetyl alcohol, glyceryl stearate citrate, cetearyl alcohol, glyceryl caprylate, glyceryl stearate, dimethicone, isosorbide dicaprylate, glycerin, erythritol, hibiscus sabdariffa fruit extract, polysilicone-11, butyrospermum parkii butter, bis-diglycer I polyacyladipate-2. stearic acid, phenoxyethanol, ethylhexylglycerin, glycerin, propanediol, collagen amino acids, haernatococcus pluvialis extract, squalane, euphorbia cerifera wax, beta-sitosterol, oleic acid, glyceryl behenate, polyglyceryl-4 isostearate, lecithin, ceramide NG, ceramide NP, tocopherol, ethylhexyl palmitate, trihydroxystearin, sodium hyaluronate, glucomannan, sodium hydroxide, sodium acrylates copolymer, carbomer, xanthan gum, hydroxyapatite, tocopheryl acetate, and disodium EDTA.

[0056] In some embodiments, the cosmetic composition consists of water, isododecane, butylene glycol phenyl trimethicone, 1,2-hexanediol, sodimn hyaluronate, cetyl alcohol, glycery l stearate citrate, cetearyl alcohol, glyceryl caprylate, glyceryl stearate, dimethicone, isosorbide dicaprylate, glycerin, erythritol, hibiscus sabdariffa fruit extract, polysilicone-11, butyrospermum parkii butter, bis-diglyccryl polyacyladipate-2, stearic acid, phenoxyethanol, ethylhexylglycerin, glycerin, propanediol, collagen amino acids, haernatococcus pluvialis extract, squalane, euphorbia cerifera wax, beta-sitosterol, oleic acid, glycery l behenate, polyglyceryl-4 isostearate, lecithin, ceramide NG. ceramide NP, tocopherol, ethylhexyl palmitate, trihydroxy stearin, sodium hyaluronate, glucomannan, sodium hydroxide, sodium acr lates copolymer, carbomer, xanthan gmn, hydroxy apatite, tocophery l acetate, and disodium EDTA.

[0057] In some embodiments, the cosmetic composition includes water, propanediol, 1,2-hexanediol, caprylhydroxamic acid, PPG-24-Glycereth-24, glycerin, erythritol, hibiscus sabdariffa fruit extract, ethylhexyl palmitate, trihydroxystearin, sodium hyaluronate, glucomannan, propanediol, collagen amino acids, haematococcus pluvialis extract, sodium poly acryloyldimethyl taurate, hydroxyapatite, sodium acry lates copolymer, lecithin, and disodium EDTA.

[0058] For example, in some embodiments, the cosmetic composition consists essentially of water, propanediol. 1,2-hexanedioL caprylhydroxamic acid. PPG-24-Glycereth-24, glycerin, erythritol, hibiscus sabdariffa fruit extract, ethylhexyl palmitate, trihydroxy stearin, sodium hyaluronate, glucomannan, propanediol, collagen amino acids, haematococcus pluvialis extract, sodium polyacryloyldimethyl taurate. hydroxyapatite, sodium acrylates copolymer, lecithin, and disodium EDTA.

[0059] In some embodiments, the cosmetic composition consists essentially of water, propanediol, 1,2-hcxancdiol, caprylhydroxamic acid, PPG-24-Glyccrcth-24, glycerin, ery thritol, hibiscus sabdariffa fruit extract, ethylhexyl palmitate, trihydroxystearin, sodium hyaluronate, glucomannan, propanediol, collagen amino acids, haematococcus pluvialis extract, sodium polyacryloyldimethyl taurate, hydroxyapatite, sodium acrylates copolymer, lecithin, and disodium EDTA.

[0060] Each component of the cosmetic composition and its respective amount in the cosmetic composition can be chosen such that the cosmetic composition is effective to improve one or more skin property.

[0061] For example, in some embodiments, the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to increase skin hydration by as determined by corneometry.

[0062] In some embodiments, the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to increase skin hydration by 13% to 150% compared to baseline as determined by corneometry’.

[0063] In some embodiments, the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to provide a statistically significant increase in skin hydration with a p-value of less than 0.05 compared to baseline as determined by comeometry.

[0064] In some embodiments, the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to increase skin conductance.

[0065] In some embodiments, the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to increase skin conductance by 100% to 600% compared to baseline.

[0066] In some embodiments, the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to provide a statistically significant increase in skin conductance with a p-value of less than 0.05 compared to baseline.

[0067] In some embodiments, the haematococcus pluvialis extract, a calcium ion delivery7agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to decrease trans epidermal water loss.

[0068] In some embodiments, the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to decrease trans epidermal water loss by 7% to 20% compared to baseline.

[0069] In some embodiments, the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to provide a statistically significant decrease in trans epidermal water with a p- value of less than 0.05 compared to baseline.

[0070] Formulations

[0071] The composition can be formulated in various forms such as, for example, a cream or a serum to facilitate application of the composition to the skin. In various implementations, the composition is a topical composition. In some embodiments, the composition is a cosmetic composition.

[0072] For example, the composition can comprise water-soluble solvents such as, for example, alcohols such as ethanol propanol, isopropanol, glycerin, and mixtures thereof. The cosmetic composition can comprise water immiscible solvents such as oils and waxes. As used herein, an oil is a water insoluble solvent such as mineral oil. vegetable oils and silicone oils, such as dimethicone and cyclomethicone. In some embodiments, the cosmetic composition includes water and / or water soluble solvents, and oils and / or water immiscible solvents.

[0073] The composition can be an emulsion, such as an oil-in-water emulsion or water-in-oil emulsion. The oil in the emulsion may be a carbon or hydrocarbon-based oil or a silicone based oil, i.e. a silicone emulsion. The cosmetic compositions can also be a solution, for example an aqueous solution, or a suspension in water or oil.

[0074] In some embodiments, the cosmetic composition is an oil-in-water emulsion containing about 60 wt% - 90 wt% water and water-soluble components and about 10 wt% to about 40 wt% components forming a water immiscible or oil phase.

[0075] In some embodiment embodiments, the composition is a serum or cream. In some embodiments, the serums has a light low viscosity, non-greasy finish and high concentrations of active ingredients.

[0076] Methods

[0077] Disclosed are methods of moisturizing skin, wherein the methods include applying a cosmetic composition disclosed herein to the skin.

[0078] In some embodiments, the cosmetic composition is applied to the skin at an amount and frequency that is effective to improve one or more skin properties. For example, in some embodiments, the method includes applying an effective amount of the cosmetic composition to the skin once per day. In some embodiments, the method includes applying an effective amount of the cosmetic composition to the skin twice per day. In some embodiments, the method includes applyingan effective amount of the cosmetic composition to the skin three times per day. In some embodiments, the method includes applying an effective amount of the cosmetic composition to the skin four or more times per day.

[0079] In some embodiments, applying the cosmetic composition to the skin is effective to increase hydration of tire skin by as determined by comeometry .

[0080] In some embodiments, applying the cosmetic composition to the skin is effective to increase hydration of the skin by 13% to 150% compared to baseline as determined by comeometry.

[0081] In some embodiments, applying the cosmetic composition to the skin is effective to statistically significant increase hydration of the skin with a p-value of less than 0.05 compared to baseline as determined by comeometry.

[0082] In some embodiments, applying the cosmetic composition to the skin is effective to increase conductance of the skin.

[0083] In some embodiments, applying the cosmetic composition to the skin is effective to increase conductance of the skin by 100% to 600% compared to baseline.

[0084] In some embodiments, applying the cosmetic composition to the skin is effective to statistically significant increase conductance of the skin with a p-value of less than 0.05 compared to baseline.

[0085] In some embodiments, applying the cosmetic composition to the skin is effective to decrease trans epidermal water loss of the skin.

[0086] In some embodiments, applying the cosmetic composition to the skin is effective to decrease trans epidermal water loss of the skin by 7% to 20% compared to baseline.

[0087] In some embodiments, applying the cosmetic composition to the skin is effective to statistically significant decrease trans epidermal water of the skin with a p-value of less than 0.05 compared to baseline.

[0088] The cosmetic composition can be applied to any skin type. For example, in some embodiments, applying the cosmetic composition to the skin is effective for each Fitzpatrick skin typeII-VI.

[0089] The following are additional nonlimiting embodiments of the disclosure:

[0090] 1. A cosmetic composition comprising haematococcus pluvialis extract, optionally a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof.

[0091] 2. The cosmetic composition according to embodiment 1, wherein the composition comprises isosorbide dicaprylate.

[0092] 3. The cosmetic composition according to embodiment 1 or 2, wherein the cosmetic composition comprises from 0.1 weight percent to 5 weight percent isosorbide dicaprylate by total weight of the cosmetic composition.

[0093] 4. The cosmetic composition according to any one of embodiments 1 to 3, w herein the cosmetic composition comprises from 1 weight percent to 3 weight percent isosorbide dicaprylate by total weight of the cosmetic composition.

[0094] 5. The cosmetic composition according to any one of embodiments 1 to 4, wherein the cosmetic composition comprises about 2 weight percent isosorbidc dicaprylatc by total w eight of the cosmetic composition.

[0095] 6. The cosmetic composition according to any one of embodiments 1 to 5.wherein the composition further comprises a glucomannan.

[0096] 7. The cosmetic composition according to embodiment 6, wherein glucomannanis is konjac glucomannan.

[0097] 9. The cosmetic composition according to embodiment 6 or 7, wherein the hyaluronic acid or a salt thereof is crosslinked with the glucomannan.

[0098] The cosmetic composition according to any one of embodiments 1 to 6, 8 wherein the calcium ion delivery agent comprises hydroxyapatite.

[0099] 10. The cosmetic composition according to any one of embodiments 1 to 9, wherein the cosmetic composition comprises from 0.01 weight percent to 1 w eight percent hydroxyapatite by total weight of the cosmetic composition.

[0100] 11. The cosmetic composition according to any one of embodiments 1 to 10, wherein the cosmetic composition comprises from 0.05 weight percent to 0.5 weight percent hydroxyapatite by total weight of the cosmetic composition.

[0101] 12. The cosmetic composition according to any one of embodiments 1 to 11, wherein the cosmetic composition comprises from about 0.1 weight percent to about 0.3 weight percent hydroxyapatite by total weight of the cosmetic composition.

[0102] 13. The cosmetic composition according to any one of embodiments 1 to 12. wherein the cosmetic composition comprises from 0.01 weight percent to 1 weight percent haematococcus pluvialis extract by total weight of the cosmetic composition.

[0103] 14. The cosmetic composition according to any one of embodiments 1 to 13, wherein the cosmetic composition comprises from 0.01 weight percent to 0.2 weight percent haematococcus pluvialis extract by total weight of the cosmetic composition.

[0104] 15. The cosmetic composition according to any one of embodiments 1 to 14, wherein the cosmetic composition comprises from about 0.04 weight percent to about 0.15 weight percent haematococcus pluvialis extract by total weight of the cosmetic composition.

[0105] 16. The cosmetic composition according to any one of embodiments 1 to 15, wherein the lipid mixture comprises two or more lipids chosen from squalane, euphorbia cerifera wax, butyrospermum parkii butter, beta-sitosterol, oleic acid, glyceryl behenate, polyglyceryl-4 isostearate, lecithin, a ceramide, tocopherol, ethylhexyl palmitate, stearic acid, and trihydroxystearin.

[0106] 17. The cosmetic composition according to any one of embodiments 1 to 16, wherein the lipid mixture comprises three or more lipids chosen from squalane, euphorbia cerifera wax, butyrospermum parkii butter, beta-sitosterol, oleic acid, glyceryl behenate, polyglyceryl-4 isostearate, lecithin, a ceramide, tocopherol, ethylhexyl palmitate, stearic acid, and trihydroxystearin.

[0107] 18. The cosmetic composition according to any one of embodiments 1 to 17, wherein the lipid mixture comprises lecithin, ethylhexyl palmitate, and trihydroxy stearin.

[0108] 19. The cosmetic composition according to any one of embodiments 1 to 18, wherein the lipid mixture comprises squalane, euphorbia cerifera wax. butyrospermum parkii butter, beta-sitosterol,oleic acid, glycery l behenate, poly gly eery 1-4 isostearate, lecithin, a ceramide, tocopherol, ethylhexyl palmitate, stearic acid, and trihydroxystearin.

[0109] 20. The cosmetic composition according to any one of embodiments 1 to 19, wherein the cosmetic composition comprises from 0.01 weight percent to 0.1 weight percent lecithin, from 0.1 weight percent to 5 weight percent ethylhexyl palmitate, and from 0.001 weight percent to 0.1 weight percent trihydroxystearin by total weight of the cosmetic composition.

[0110] 21. The cosmetic composition according to any one of embodiments 1 to 20, wherein the cosmetic composition comprises from 0.1 weight percent to 0.5 weight percent squalane, from 0.01 weight percent to 0.1 weight percent euphorbia cerifera wax, from 0.01 weight percent to 2 weight percent butyrospermum parkii butter, from 0.01 weight percent to 0.1 weight percent beta-sitosterol, from 0.01 weight percent to 0.1 weight percent oleic acid, from 0.01 weight percent to 0.1 weight percent glyceryl behenate, from 0.01 weight percent to 0.05 weight percent polyglyceiyl-4 isostearate, from 0.01 weight percent to 0.1 weight percent lecithin, from 0.1 weight percent to 5 weight percent ethylhexyl palmitate, from 0.1 weight percent to 3 weight percent stearic acid, and from 0.001 weight percent to 0.1 weight percent trihydroxy stearin by total weight of the cosmetic composition.

[0111] 22. The cosmetic composition according to any one of embodiments 1 to 21, wherein the cosmetic composition comprises from 0.001 weight percent to 0.03 weight percent hibiscus sabdariffa fruit extract by total weight of the cosmetic composition.

[0112] 23. The cosmetic composition according to any one of embodiments 1 to 22, wherein the cosmetic composition comprises from 0.001 weight percent to 0.1 weight percent hyaluronic acid or a salt thereof by total weight of the cosmetic composition.

[0113] 24. The cosmetic composition according to any one of embodiments 1 to 23. wherein the cosmetic composition comprises from 0.001 weight percent to 0.1 weight percent sodium hyaluronate by total weight of the cosmetic composition.

[0114] 25. The cosmetic composition according to any one of embodiments 1 to 24, wherein the cosmetic composition comprises isododecane.

[0115] 26. The cosmetic composition according to any one of embodiments 1 to 25, wherein the cosmetic composition comprises from 1 weight percent to 10 weight percent isododecane by total weight of the cosmetic composition.

[0116] 27. The cosmetic composition according to any one of embodiments 1 to 26, wherein the cosmetic composition comprises butylene glycol.

[0117] 28. The cosmetic composition according to any one of embodiments 1 to 27, wherein the cosmetic composition comprises from 1 weight percent to 10 weight percent butylene glycol by total weight of the cosmetic composition.

[0118] 29. The cosmetic composition according to any one of embodiments 1 to 28, wherein the cosmetic composition comprises phenyl trimethicone.

[0119] 30. The cosmetic composition according to any one of embodiments 1 to 29, wherein the cosmetic composition comprises from 1 weight percent to 10 weight percent phenyl trimethicone by total weight of the cosmetic composition.

[0120] 31. The cosmetic composition according to any one of embodiments 1 to 30, wherein the cosmetic composition comprises cetyl alcohol.

[0121] 32. The cosmetic composition according to any one of embodiments 1 to 31, wherein the cosmetic composition comprises from 1 weight percent to 10 weight percent cetyl alcohol by total weight of the cosmetic composition.

[0122] 33. The cosmetic composition according to any one of embodiments 1 to 32, wherein the cosmetic composition comprises glyceryl stearate citrate, cetearyl alcohol, and glycery l caprylate.

[0123] 34. The cosmetic composition according to any one of embodiments 1 to 33, wherein the cosmetic composition comprises from 0.1 weight percent to 5 weight percent glyceryl stearate citrate, from 0.1 weight percent to 3 weight percent cetearyl alcohol, and from 0.1 weight percent to 3 weight percent glyceryl caprylate by total weight of the cosmetic composition.

[0124] 35. The cosmetic composition according to any one of embodiments 1 to 34, wherein the cosmetic composition comprises glycery l stearate.

[0125] 36. The cosmetic composition according to any one of embodiments 1 to 35, wherein the cosmetic composition comprises from 0.1 weight percent to 5 weight percent glyceryl stearate by total weight of the cosmetic composition.

[0126] 37. The cosmetic composition according to any one of embodiments 1 to 36, wherein the cosmetic composition comprises dimethicone.

[0127] 38. The cosmetic composition according to any one of embodiments 1 to 37, wherein the cosmetic composition comprises from 0.1 weight percent to 5 weight percent dimethicone by total weight of the cosmetic composition.

[0128] 39. The cosmetic composition according to any one of embodiments 1 to 38, wherein the cosmetic composition comprises erythritol.

[0129] 40. The cosmetic composition according to any one of embodiments 1 to 39, wherein the cosmetic composition comprises from 0.01 weight percent to 0.5 weight percent erythritol by total weight of the cosmetic composition.

[0130] 41. The cosmetic composition according to any one of embodiments 1 to 40, wherein the cosmetic composition comprises polysilicone-11.

[0131] 42. The cosmetic composition according to any one of embodiments 1 to 41, wherein the cosmetic composition comprises from 0.01 weight percent to 0.5 weight percent polysilicone-11 by total weight of the cosmetic composition.

[0132] 43. The cosmetic composition according to any one of embodiments 1 to 42, wherein the cosmetic composition comprises bi s-digly ceryl polyacyladipate-2.

[0133] 44. The cosmetic composition according to any one of embodiments 1 to 43, wherein the cosmetic composition comprises from 0.1 weight percent to 5 weight percent bis-diglyccr I polyacyladipate-2 by total weight of the cosmetic composition.

[0134] 45. The cosmetic composition according to any one of embodiments 1 to 44. wherein the cosmetic composition comprises phenoxyethanol and ethylhexylglycerin.

[0135] 46. The cosmetic composition according to any one of embodiments 1 to 45, wherein the cosmetic composition comprises from 0.1 weight percent to 2 weight percent phenoxyethanol andfrom 0.01 weight percent to 0.2 weight percent ethylhexylglycerin by total weight of the cosmetic composition.

[0136] 47. The cosmetic composition according to any one of embodiments 1 to 46, wherein the cosmetic composition comprises collagen amino acids.

[0137] 48. The cosmetic composition according to any one of embodiments 1 to 47, wherein the cosmetic composition comprises from 0.1 weight percent to 5 weight percent collagen amino acids by total weight of the cosmetic composition.

[0138] 49. The cosmetic composition according to any one of embodiments 1 to 48, wherein the cosmetic composition comprises tocopheryl acetate.

[0139] 50. The cosmetic composition according to any one of embodiments 1 to 49, wherein the cosmetic composition comprises from 0.01 weight percent to 0.5 weight percent tocopheryl acetate by total weight of the cosmetic composition.

[0140] 51. The cosmetic composition according to any one of embodiments 1 to 50, wherein the cosmetic composition comprises caprylhydroxamic acid.

[0141] 52. The cosmetic composition according to any one of embodiments 1 to 51. wherein the cosmetic composition comprises from 0.01 weight percent to 0.5 weight percent caprylhydroxamic acid by total weight of the cosmetic composition.

[0142] 53. The cosmetic composition according to any one of embodiments 1 to 52, wherein the cosmetic composition comprises PPG-24-Glycereth-24.

[0143] 54. The cosmetic composition according to any one of embodiments 1 to 53, wherein the cosmetic composition comprises from 1 weight percent to 5 weight percent PPG-24-Glycereth-24 by total weight of the cosmetic composition.

[0144] 55. The cosmetic composition according to any one of embodiments 1 to 54, wherein the cosmetic composition comprises sodium polyacryloyldimethyl taurate.

[0145] 56. The cosmetic composition according to any one of embodiments 1 to 55, wherein the cosmetic composition comprises from 0.1 weight percent to 2 weight percent sodium polyacryloyldimethyl taurate by total weight of the cosmetic composition.

[0146] 57. The cosmetic composition according to any one of embodiments 1 to 56, wherein the cosmetic composition comprises at least 60 weight percent water by total weight of the cosmetic composition.

[0147] 58. The cosmetic composition according to any one of embodiments 1 to 57, wherein the cosmetic composition comprises at least 85 weight percent water by total weight of the cosmetic composition.

[0148] 59. The cosmetic composition according to any one of embodiments 1 to 58. wherein the cosmetic composition comprises from 60 weight percent to 90 weight percent water by total weight of the cosmetic composition.

[0149] 60. The cosmetic composition according to any one of embodiments 1 to 59, wherein the cosmetic composition is a cream.

[0150] 61. The cosmetic composition according to any one of embodiments 1 to 59, wherein the cosmetic composition is a serum.

[0151] 62. The cosmetic composition according to any one of embodiments 1 to 61, wherein the cosmetic composition consists essentially of water, isododecane, butylene glycol phenyl trimethicone, 1.2-hexanediol, sodium hyaluronate, cetyl alcohol, glyceryl stearate citrate, cetearyl alcohol, glyceryl caprylate, glyceryl stearate, dimethicone, isosorbide dicaprylate, glycerin, erythritol, hibiscus sabdariffa fruit extract, po ly silicone- 11, butyrospermum parkii butter, bis-diglyceryl polyacyladipate- 2, stearic acid, phenoxyethanol, ethylhexylglycerin, glycerin, propanediol, collagen amino acids, haematococcus pluvialis extract, squalane, euphorbia cerifera wax, beta-sitosterol, oleic acid, glyceryl behenate, polyglyceryl-4 isostearate, lecithin, ceramide NG, ceramide NP, tocopherol, ethylhexyl palmitate, trihydroxystearin, sodium hyaluronate, glucomannan, sodium hydroxide, sodium acr lates copolymer, carbomer, xanthan gum, hydroxyapatite, tocopheryl acetate, and disodium EDTA.

[0152] 63. The cosmetic composition according to any one of embodiments 1 to 62, wherein the cosmetic composition consists of water, isododecane, butylene glycol phenyl trimethicone, 1,2- hexanediol, sodium hyaluronate, cetyl alcohol, glyceryl stearate citrate, cetearyl alcohol, glyceryl caprylate, glyceryl stearate, dimethicone, isosorbide dicaprylate, glycerin, erythritol, hibiscussabdariffa fruit extract, po ly silicone- 11, biityrospcrinum parkii butter, bis-diglyceryl polyacyladipate- 2, stearic acid, phenoxyethanol, ethylhexylglycerin, glycerin, propanediol, collagen amino acids, haematococcus pluvialis extract, squalane, euphorbia cerifera wax. beta-sitosterol, oleic acid, glyceryl behenate, poly gly eery 1-4 isostearate, lecithin, ceramide NG, ceramide NP, tocopherol, ethylhexyl palmitate, trihydroxystearin, sodium hyaluronate, glucomannan, sodium hydroxide, sodium acry lates copolymer, carbomer, xanthan gum. hydroxyapatite, tocophcr l acetate, and disodium EDTA.

[0153] 64. The cosmetic composition according to any one of embodiments 1 to 61, wherein the cosmetic composition consists essentially of water, propanediol, 1,2-hexanediol, caprylhydroxamic acid, PPG-24-Glycereth-24, glycerin, erythritol, hibiscus sabdariffa fruit extract, ethylhexyl palmitate, trihydroxyrstearin, sodium hyaluronate, glucomannan, propanediol, collagen amino acids, haematococcus pluvialis extract, sodium polyacryloyldimethyl taurate, hydroxyapatite, sodium acry lates copolymer, lecithin, and disodium EDTA.

[0154] 65. The cosmetic composition according to any one of embodiments 1 to 61, wherein the cosmetic composition consists of water, propanediol. 1.2-hexanediol, caprylhydroxamic acid. PPG- 24-Glycereth-24, glycerin, erythritol, hibiscus sabdariffa fruit extract, ethylhexyl palmitate, trihydroxy' stearin, sodium hyaluronate, glucomannan, propanediol, collagen amino acids, haematococcus pluvialis extract, sodium polyacryloyldimethyl taurate, hydroxy apatite, sodium acry lates copolymer, lecithin, and disodium EDTA.

[0155] 66. The cosmetic composition according to any one of embodiments 1 to 65, wherein the haematococcus pluvialis extract, a calcium ion delivery' agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to increase skin hydration by as determined by comeometry.

[0156] 67. The cosmetic composition according to any one of embodiments 1 to 66, wherein the haematococcus pluvialis extract, a calcium ion delivery' agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to increase skin hydration by 13% to 150% compared to baseline as determined by corneometry.

[0157] 68. The cosmetic composition according to any one of embodiments 1 to 67, wherein the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to provide a statistically significant increase in skin hydration with a p-value of less than 0.05 compared to baseline as determined by comcomctry.

[0158] 69. The cosmetic composition according to any one of embodiments 1 to 68, wherein the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to increase skin conductance.

[0159] 70. The cosmetic composition according to any one of embodiments 1 to 69, wherein the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to increase skin conductance by 100% to 600% compared to baseline.

[0160] 71. The cosmetic composition according to any one of embodiments 1 to 70, wherein the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to provide a statistically significant increase in skin conductance with a p-value of less than 0.05 compared to baseline.

[0161] 72. The cosmetic composition according to any one of embodiments 1 to 71, wherein the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to decrease trans epidermal water loss.

[0162] 73. The cosmetic composition according to any one of embodiments 1 to 72, wherein the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipid mixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to decrease trans epidermal water loss by 7% to 20% compared to baseline.

[0163] 74. The cosmetic composition according to any one of embodiments 1 to 73, wherein the haematococcus pluvialis extract, a calcium ion delivery agent, hibiscus sabdariffa fruit extract, a lipidmixture, and hyaluronic acid or a salt thereof are chosen in amounts effective to provide a statistically significant decrease in trans epidermal water with a p-value of less than 0.05 compared to baseline.

[0164] 75. A method of moisturizing skin comprising applying a cosmetic composition according to any one of embodiments 1 to 74 to the skin.

[0165] 76. The method according to embodiment 75, wherein applying the cosmetic composition to the skin is effective to increase hydration of the skin by as determined by comeometry.

[0166] 77. The method according to embodiment 75 or 76, wherein applying the cosmetic composition to the skin is effective to increase hydration of tire skin by 13% to 150% compared to baseline as determined by comeometry.

[0167] 78. The method according to any one of embodiments 75 to 77. wherein applying the cosmetic composition to the skin is effective to statistically significant increase hydration of the skin with a p-value of less than 0.05 compared to baseline as determined by comeometry.

[0168] 79. The method according to any one of embodiments 75 to 78, wherein applying the cosmetic composition to the skin is effective to increase conductance of the skin.

[0169] 80. The method according to any one of embodiments 75 to 79. wherein applying the cosmetic composition to the skin is effective to increase conductance of the skin by 100% to 600% compared to baseline.

[0170] 81. The method according to any one of embodiments 75 to 80. wherein applying the cosmetic composition to the skin is effective to statistically significant increase conductance of the skin with a p-value of less than 0.05 compared to baseline.

[0171] 82. The method according to any one of embodiments 75 to 81, wherein applying the cosmetic composition to the skin is effective to decrease trans epidermal water loss of the skin.

[0172] 83. The method according to any one of embodiments 75 to 82. wherein applying the cosmetic composition to the skin is effective to decrease trans epidermal water loss of the skin by 7% to 20% compared to baseline.

[0173] 84. The method according to any one of embodiments 75 to 83, wherein applying the cosmetic composition to the skin is effective to statistically significant decrease trans epidermal water of the skin with a p-value of less than 0.05 compared to baseline.

[0174] 85. The method according to any one of embodiments 75 to 84, wherein the method is effective for each Fitzpatrick skin type II- VI.

[0175] 86. The method according to any one of embodiments 75 to 85. wherein the skin is free from clinically significant skin pathologies.

[0176] 87. The method according to any one of embodiments 75 to 86, wherein the skin is free from acne, active atopic dermatitis, eczema, and psoriasis.

[0177] 88. The method according to any one of embodiments 75 to 87, wherein the skin is free from cancer.

[0178] EXAMPLES

[0179] The invention is illustrated by the following non-limiting examples

[0180] Example Moisturizer Formulation as used in the Clinical Efficacy Study 1

[0181] Example Serum Formulation as used in the Clinical Efficacy Study 1

[0182] Clinical Efficacy Study 1

[0183] Twenty-one (21) female subjects aged 25 to 65 having a Fitzpatrick skin type II- VI completed the study. The study did not include subject with acne, active atopic dermatitis, eczema, psoriasis, or a treatment history for cancer.

[0184] During the 7-day washout period only neutral, non-moisturizing cleanser was used. No moisturizer was permitted.

[0185] At baseline, test sites of 5 X 5 cm were marked on the legs, then 0.2 5ml of each test product was applied to tire assigned test site with one site reserved as the untreated control. In addition, a test site was treated with the combination of Moisturizer applied first and allow to dry. followed by Serum.

[0186] At Baseline. 30 minutes post-application, and hours 8, 24, 48, and 72 hours triplicate Comeometer measurements were taken on the center of each test site (adjacent locations), triplicate Skicon measurements were taken on the center of each test site (adjacent locations) and a single Tewameter measurement were performed on the center of each test site on the subject’s left and right legs.

[0187] Results

[0188] Comeometer®1

[0189] *Values different from baseline (p<0.05)

[0190] Each subject had an average baseline Comeometer® measurement < 40 a.u.

[0191] Skin Conductance (SkiCon)

[0192] * Values statistically different from baseline (p<0.05).

[0193] Tewameter® (Trans Epidermal Water Loss)

[0194] * Values different from baseline

[0195] Example 2

[0196] Four different formulations were constructed. Fonnulation 1, a serum, comprised 1.2-Hexanediol, Capr lhydroxamic Acid, Erythritol, Ethylhexyd Palmitate, Glucomannan, Glycerin,Glycery l Oleate Citrate, Haematococcus Pluvialis Extract, Hibiscus Sabdariffa Fruit Extract,Hydrolyzed Haematococcus Pluvialis Extract, Hydroxyapatite, PPG-24-Glycereth-24, Propanediol, Sodium Hyaluronate. Sodium Polyacryloyldimethyl Taurate, Trihydroxystearin. Trisodium Ethylenediamine Disuccinate, and Water. Formulation 2, a moisturizer, comprised 1,2-Hexanediol, Beta-Sitosterol, Bis-Digly ceryl Polyacyladipate-2. Butylene Glycol, Butyrospennum Parkii (Shea) Butter, Carbomer, Ceramide NG, Ceramide NP, Cetearyl Alcohol, Cety l Alcohol, Dimethicone, Disodium EDTA, Erythritol, Ethylhexyl Palmitate. Ethylhexylglycerin, Euphorbia Cerifera (Candelilla) Wax, Glucomannan. Glycerin, Glyceryl Behenate. Glyceryl Caprylate, Glyceryl Stearate, Glyceryl Stearate Citrate, Haematococcus Pluvialis Extract, Hibiscus Sabdariffa Fruit Extract, Hydrolyzed Haematococcus Pluvialis Extract, Hydroxyapatite, Isododecane, Isosorbide Dicaprylate, Lecithin, Oleic Acid, Phenoxyethanol, Phenyl Trimethicone. Polyglyceryl-4 Isostearate. Polysilicone- 11, Propanediol. Sodium Acrylates Copolymer, Sodium Hyaluronate, Sodium Hydroxide, Squalane, Stearic Acid, Tocopherol, Tocopheryl Acetate, Trihydroxystearin, Water, and Xanthan Gum. Formulation 3 comprised 1.2-Hexanediol, Allantoin. Butyrospennum Parkii (Shea) Butter. Caprylyl Glycol, Ceramide AP, Ceramide EOP, Ceramide NP, Cetearyl Alcohol, Cetearyl Glucoside. Cetyl Alcohol, Cholesterol, Citric Acid, Dihydroxy Methylchromone, Dimethicone. Ethyl Linoleate, Ethyl Linolenate, Ethyl Oleate, Ethyl Palmitate, Ethyl Stearate, Fructose, Glucose, Glycerin, Glyceryl Stearate. Glyceryl Stearate SE, Glycosphingolipids, Helianthus Annuus (Sunflower) Seed Wax. Hydrogenated Lecithin, Hydrogenated Poly decene, Hydroxyapatite, Isopropyl Palmitate, Isosorbide Dicapry late, Jojoba Oil / Macadamia Seed Oil Esters, Maltose, Pcntacrythrityl Tctra-di-t-butyl Hydroxyhydrocinnamate. Pentylene Glycol, Phenylpropanol. Phytosphingosine. Phytosterols, Phytosteryl Macadamiate, Polysorbate 20, Propanediol, Retinol, Sodium Chloride, Sodium Hyaluronate, Sodium Hydroxide, Sodium Lactate, Sodium PCA, Squalene, Stearic Acid, Tetraacetylphytosphingosine. Tocopherol. Trehalose, Urea, Water, and Xanthan Gum. Formulation 4 comprised 1.2-Hexanediol, Caprvlhydroxamic Acid, Cetearyl Olivate. Cetyl Alcohol, Dimer Dilinoleyl Dimer Dilinoleate, Dimethicone, Erythritol. Glycerin, Haematococcus Pluvialis Extract. Hibiscus Sabdariffa Fruit Extract, Hydrolyzed Haematococcus Pluvialis Extract, Isopropyl Palmitate, Palmitic Acid, Polyacrylate Crosspolymer-6, Propanediol, Sodium Hyaluronate. Sorbitan Olivate,Stearic Acid, and Water.

[0197] Weight Percentages of AQP3 stimulator, calcium ion delivery agent, osmolytic delivery agent, and hyaluronic acid (or salt thereof) for Formulations 1-4.

[0198] Example 3

[0199] Formulation 3 was tested in a clinical study to test the effect of a cosmetic product on the level of hydration on the upper layers of the epidermis using a Comeometer® CM825, and the effect on skin barrier function by determining the transepidennal water loss (TEWL), using a Tewameter® TM HEX. Formulation 3 was applied once and measurements were taken at 15 minutes. 4 horns, 8 days, 24 hours, 48 hours. 72 hours and 100 hours following application. The clinical study was taken on 30 female subjects aged 18 to 65 years of age having an average baseline Corneometer® CM825 measurement of < 40 on legs (which may be characterized as dry and / or very dry ).

[0200] Each participant had 2 mg / cm2of Formulation 3 applied to an area of 4 cm *7 cm and, following rest of each subject for 30 minutes in an environment with 45 ± 5% relative humidity and a temperature of 21 ± 1°C. measurements were performed at each time point.

[0201] Results

[0202] Transepidermal water loss (TEWL): mean and standard error of the mean as measured by theTewameter® TM HexDifferences of the transepidermal water loss over time A(Tx-TO)Variation percentages of the transepidermal water loss between the control area and the treated area, at different measurement times

[0203] Electric capacitance: mean and standard error of the mean as measured by theComeometer®CM8250204] Differences of the electric capacitance over time A(Tx-TO)

[0205] Variation percentages of the moisturizing degree between the control area and the treated area, at different measurement timesExample 4Formulation 4 was tested in a clinical study involving the lower legs of 32 healthy, adult female subjects between the ages of 18-70 years old. Skin hydration and transepidermal water loss were measured over a 100 hour period similar to as described in Example 3.ResultsMeasured Skin Hydration via Comeometer (positive differences indicates the test product site had better skin hydration that control (untreated) site*p<0.05Measured Trans Epidermal Water Loss (negative differences indicates the test product site had better skin barrier function that control (untreated) site*p<0.05, NS=Not Significant

[0206] All examples presented are representative and non-limiting. The above-described embodiments may be modified or varied, without departing from the disclosure, as appreciated by those skilled in the art.

Claims

CLAIMS1. A cosmetic composition comprising an aquaporin 3 (AQP3) stimulator, optionally a calcium ion delivery agent, an osmolytic transport stimulator, a lipid mixture, and hyaluronic acid or a salt thereof.

2. The cosmetic composition according to claim 1, wherein the calcium ion delivery agent comprises hydroxy apatite.

3. The cosmetic composition according to claim 1 or 2, wherein the AQP3 stimulator is selected from the group consisting of haematococcus pluvialis extract, isosorbide dicaprylate, or a combination thereof.

4. The cosmetic composition according to claim 1 or 2, wherein the AQP3 stimulator is haematococcus pluvialis extract and the cosmetic composition comprises from 0.01 weight percent to 1 weight percent haematococcus pluvialis extract by total weight of the cosmetic composition, and / or isosorbide dicaprylate in an amount from 0.5 weight percent to 2.5 weight percent.

5. The cosmetic composition according to any one of claims 1-4, wherein the lipid mixture comprises two or more lipids chosen from squalane, euphorbia cerifera wax, butyrospennum parkii butter, beta-sitosterol, oleic acid, glyceryl behenate, polyglyceryl-4 isostearate, lecithin, a ceramide, tocopherol, ethylhexyl palmitate, stearic acid, and trihydroxystearin.

6. The cosmetic composition according to claim 5, wherein the lipid mixture comprises lecithin, ethyl hex l palmitate, and trihydroxy stearin.

7. The cosmetic composition according to claim 5, wherein the lipid mixture comprises squalane, euphorbia cerifera wax, butyrospennum parkii butter, beta-sitosterol, oleic acid, glycerylbehenate, polyglyceryl-4 isostearate, lecithin, a ceramide, tocopherol, ethylhexyl palmitate, stearic acid, and trihydroxystearin.

8. The cosmetic composition according to any one of claims 1-7, wherein the osmolytic transport stimulator is hibiscus sabdariffa fruit extract, trehalose, or a combination thereof.

9. The cosmetic composition according to claim 8, wherein the composition comprises from 0.001 weight percent to 0.03 weight percent hibiscus sabdariffa fruit extract by total weight of the cosmetic composition and / or 0.001 weight percent to 0.1 weight percent trehalose by weight of the composition.

10. The cosmetic composition according to any one of claims 1-9, wherein the cosmetic composition comprises from 0.001 weight percent to 0.1 weight percent hyaluronic acid or a salt thereof by total weight of the cosmetic composition.

11. The cosmetic composition according to any one of claims 1-10. wherein the cosmetic composition further comprises isododecane.

12. The cosmetic composition according to any one of claims 1-11, wherein the cosmetic composition further comprises cetyl alcohol.

13. The cosmetic composition according to any one of claims 1-12, wherein the cosmetic composition further comprises glyceryl stearate citrate, ceteary 1 alcohol, and glycery l capry late.

14. The cosmetic composition according to any one of claims 1-14, wherein the cosmetic composition further comprises dimethicone.

15. The cosmetic composition according to any one of claims 1-15. wherein the cosmetic composition further comprises erythritol.

16. The cosmetic composition according to any one of claims 1-15. wherein the cosmetic composition further comprises polysilicone-11.

17. The cosmetic composition according to any one of claims 1-16, wherein the cosmetic composition further comprises bis-diglyceryl polyacyladipate-2.

18. The cosmetic composition according to any one of claims 1-18, wherein the cosmetic further composition comprises phenoxyethanol and ethylhexylglycerin.

19. The cosmetic composition according to any one of claims 1-18, wherein the cosmetic composition further comprises collagen amino acids.

20. The cosmetic composition according to any one of claims 1-19, wherein the cosmetic composition is a cream or a serum.

21. A method of moisturizing skin, the method comprising applying a cosmetic composition according to any one of claims 1-20 to the skin.

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