Reactive carbon species generating agent and method for producing same, composition with increased concentration of reactive carbon species, and method for increasing concentration of reactive carbon species

Abstract

The present invention provides a novel material that generates reactive carbon species. The present invention relates to a reactive carbon species generating agent comprising a yeast cell wall-treated product.

Description

Activated carbon species generator and method for producing the same, composition with increased concentration of activated carbon species, and method for increasing the concentration of activated carbon species

[0001] The present invention relates to an activated carbon species generator and a method for producing the same, as well as a composition having an increased concentration of activated carbon species and a method for increasing the concentration of activated carbon species.

[0002] Techniques for generating reactive oxygen species are known and are widely used in air purifiers such as nanoe and plasma cluster ions. However, no technology is known for industrially and inexpensively generating activated carbon species. It is known that activated carbon species (carbon radical species) are used in processes such as the cumene process, an industrial method for producing phenol. It is also known that organic molecular catalysts can be used to generate carbon radicals. Examples of such organic molecular catalysts include N-hydroxyphthalimide, benzophenone, and eosin Y (Chemistry and Education, Vol. 64, No. 10 (2016)). On the other hand, it is not known that yeast cell wall hydrolysates (JP 2023-173502 A) contain activated carbon species (carbon radical species), and no technology is known for increasing the amount of activated carbon species (carbon radical species) in yeast cell walls.

[0003] Japanese Patent Application Laid-Open No. 2023-173502

[0004] Chemistry and Education, Vol. 64, No. 10 (2016)

[0005] The present invention aims to provide a novel material that generates activated carbon species.

[0006] As a result of extensive research, the present inventors have discovered that activated carbon species are generated by subjecting yeast cell walls to a hydrothermal reaction treatment or the like, and have thus completed the present invention. The gist of the present invention is as follows: [1] A composition comprising a treated yeast cell wall product containing 3 μmol / L or more of activated carbon species. [2] The composition according to [1] above, wherein the treated yeast cell product is a hydrothermal reaction product of yeast cell walls. [3] The composition according to [1] or [2] above, further comprising an activated carbon species generation promoter. [4] The composition according to [3] above, wherein the activated carbon species generation promoter increases the concentration of activated carbon species in the composition by about 1.4 times or more. [5] The composition according to [3] or [4] above, wherein the activated carbon species generation promoter comprises divalent iron or trivalent iron ions and / or β-glucan. [6] The composition according to any one of [1] to [5] above, wherein the β-glucan content is 4 mass% or more and the divalent iron or trivalent iron ion concentration is 10 mM or more. [7] The composition according to any one of [1] to [5] above, wherein the β-glucan content is 8% by mass or more. [8] The composition according to any one of [1] to [5] above, wherein β-glucan is added in an amount of 5% by mass or more. [9] An activated carbon species generator comprising a treated yeast cell wall product.

[10] The activated carbon species generator according to [9] above, wherein the Fe(III)-complex is contained at 0.20 mmol / L or more.

[11] The activated carbon species generator according to [9] or

[10] above, wherein the Fe(III)-complex concentration is increased by 1.3 times or more compared to the Fe(III)-complex concentration in the treated yeast cell wall product.

[12] The activated carbon species generator according to any one of [9] to

[11] above, for generating activated carbon species having a polystyrene permeation effect.

[13] An activated carbon species generation promoter for increasing the concentration of activated carbon species in a treated yeast cell wall product, wherein the activated carbon species generator comprises divalent iron or trivalent iron ions and / or β-glucan.

[14] A method for producing the activated carbon species generator according to any one of [9] to

[12] above, which comprises mixing a treated yeast cell wall product with an activated carbon species generation promoter.

[15] A method for increasing the concentration of activated carbon species in a composition containing a treated yeast cell wall product, which comprises adding divalent iron or trivalent iron ions and / or β-glucan to the composition containing the treated yeast cell wall product.

[16] An anti-cancer composition containing the composition according to any one of [1] to [8] above as an active ingredient.

[17] The anticancer composition according to

[16] , which is used by directly contacting an affected area containing cancer cells.

[18] The anticancer composition according to

[16] or

[17] , which contains activated carbon species having a polystyrene permeability effect.

[0007] According to the present invention, a novel material that generates activated carbon species can be provided. Since this novel material generates activated carbon species, it can be used for cancer treatment and the like.

[0008] Table 1 shows electron spin resonance (ESR) spectra of hydrothermally treated yeast cell walls (YCW-H) (A), YCW-H with added Fe(II) (B), and YCW-H with added Fe(III) (C), along with a table (D) summarizing the RCS and Fe(III)-complex amounts. The figure shows that YCW-H dose-dependently inhibited the proliferation of MCF7 breast cancer cells. The figure also shows the inhibitory effect of adding Fe(II) or Fe(III) to YCW-H on the proliferation of MCF7 breast cancer cells (A), and the reduction in RCS (B) and the reduction in the inhibitory effect on MCF7 breast cancer cells (C) by adding EDTA to a mixture of Fe(III) and YCW-H. The figure also shows the results of breast cancer cell proliferation inhibition by adjacent treatment with YCW-H.

[0009] The treated yeast cell wall product used in the present invention is a product of treating yeast cell walls with a hydrothermal reaction or the like, and the type of treatment is not particularly limited. The treated yeast cell wall product is preferably a hydrothermal reaction product of yeast cell walls.

[0010] In the present invention, hydrothermal reaction treatment (superheated steam treatment) refers to a method in which superheated steam is generated by heating and pressurization, and the physical properties of an object are changed by the effect of the generated superheated steam. The yeast cell walls to be treated with hydrothermal reaction treatment or the like are not particularly limited. Examples of the yeast cell walls include dried yeast cell walls and yeast cell wall suspensions. The temperature at which superheated steam is generated is preferably 120°C or higher and 220°C or lower, more preferably 150°C or higher and 210°C or lower. The pressure at which superheated steam is generated is preferably 0.9 MPa or higher and 1.9 MPa or lower, more preferably 1.2 MPa or higher and 1.8 MPa or lower. In particular, hydrothermal reaction treatments performed at a pressure of 0.9 MPa to 1.9 MPa and a temperature of 120 ° C. to 220 ° C. are preferred, hydrothermal reaction treatments performed at a pressure of 0.9 MPa to 1.9 MPa and a temperature of 150 ° C. to 210 ° C. are more preferred, and hydrothermal reaction treatments performed at a pressure of 1.2 MPa to 1.8 MPa and a temperature of 150 ° C. to 210 ° C. are even more preferred. Hydrothermal reaction-treated yeast cell walls are also disclosed, for example, in WO 2013 / 094235 and can also be prepared according to the methods disclosed in WO 2013 / 094235 and the like.

[0011] Treated yeast cell walls can generate activated carbon species, and compositions containing treated yeast cell walls can contain a predetermined amount or more of activated carbon species. Therefore, one aspect of the present invention relates to an activated carbon species generator containing a treated yeast cell wall. The activated carbon species generator of the present invention preferably contains an Fe(III)-complex at 0.20 mmol / L or more, for example, 0.25 mmol / L or more, 0.29 mmol / L or more, 0.30 mmol / L or more, 1 mmol / L or more, or 10 mmol / L or more. Furthermore, the activated carbon species generator of the present invention is preferably intended to generate activated carbon species that have a polystyrene permeation effect. That is, the activated carbon species generator of the present invention can generate activated carbon species that can pass through a polystyrene wall (membrane (film)). The activated carbon species can permeate the polystyrene wall and move between compartments completely separated by the polystyrene wall. Another aspect of the present invention relates to a composition containing treated yeast cell walls, which contains a predetermined amount or more of activated carbon species. The amount of activated carbon species contained in the composition containing the treated yeast cell wall product is preferably 3 μmol / L or more, more preferably 3 μmol / L to 85 μmol / L, and even more preferably 3.5 μmol / L to 85 μmol / L. The amount of activated carbon species contained in the composition can be determined, for example, by analysis using electron spin resonance (ESR).

[0012] In the present invention, an activated carbon species generation promoter can be used to improve the active carbon species generation ability of a yeast cell wall-treated product. That is, an activated carbon species generation promoter can be further added to a composition containing the yeast cell wall-treated product of the present invention. Furthermore, the activated carbon species generator of the present invention can contain the activated carbon species generation promoter together with the yeast cell wall-treated product. For example, the activated carbon species generation promoter can increase the amount of activated carbon species contained in the composition containing the yeast cell wall-treated product by 1.4 times or more, 2 times or more, 3 times or more, 4 times or more, or 5 times or more compared to the amount of activated carbon species contained in the composition containing the yeast cell wall-treated product before the addition of the activated carbon species generation promoter. Therefore, it can be used to increase the concentration of activated carbon species in the yeast cell wall-treated product. Note that the active carbon species generation ability of the yeast cell wall-treated product may decrease over time depending on storage conditions. Even when used on a yeast cell wall-treated product with reduced active carbon species generation ability, the activated carbon species generation promoter can similarly improve the active carbon species generation ability of the yeast cell wall-treated product. When used on a yeast cell wall-treated product with reduced active carbon species generation ability, the activated carbon species generation promoter can also be referred to as an activated carbon species recovery agent. That is, the active carbon species recovery agent can increase the amount of activated carbon species contained in a composition containing a treated yeast cell wall product with reduced active carbon species generation ability by 1.4 times or more, 2 times or more, 3 times or more, 4 times or more, or 5 times or more compared to the amount before the addition of the active carbon species recovery agent. Furthermore, in the present invention, the active carbon species generation promoter can be used in an activated carbon species generator to increase the Fe(III)-complex concentration in the treated yeast cell wall product. That is, the active carbon species generation promoter can increase the Fe(III)-complex concentration in the activated carbon species generator containing the treated yeast cell wall product by 1.3 times or more, 1.6 times or more, 1.9 times or more, 2 times or more, 6.7 times or more, or 67 times or more compared to the Fe(III)-complex concentration in the treated yeast cell wall product.

[0013] The activated carbon species generation promoter is not particularly limited as long as it can improve the activated carbon species generation ability of the treated yeast cell wall product. Examples of the activated carbon species generation promoter include iron (Fe) ions and β-glucan. Examples of iron (Fe) ions include divalent iron ions and trivalent iron ions. Furthermore, the compound capable of supplying iron (Fe) ions is not particularly limited. Examples of compounds capable of supplying iron (Fe) ions include ferrous sulfate, ferrous sulfate heptahydrate, ferrous chloride, ferric sulfate, ferric sulfate hydrate, and ferric chloride. In the present invention, the activated carbon species generation promoter includes not only iron (Fe) ions but also compounds capable of supplying iron (Fe) ions. Preferably, the supply of trivalent iron ions is considered. The activated carbon species generation promoter preferably contains divalent iron or trivalent iron ions and / or β-glucan.

[0014] Treated yeast cell walls, such as hydrothermal reaction products of yeast cell walls, contain β-glucan. The use of β-glucan as an activated carbon species generation promoter in addition to the above can improve the ability to generate activated carbon species. For example, by adding β-glucan as an activated carbon species generation promoter, the β-glucan content of a composition containing a treated yeast cell wall is preferably 8% by mass or more, and may be 9% by mass or more, 10% by mass or more, 11% by mass or more, or 12% by mass or more. The upper limit of the content is approximately 63% by mass, and may be 58% by mass or less, 53% by mass or less, 48% by mass or less, or 43% by mass or less. The β-glucan content of a composition can be measured, for example, as follows. First, the amount of glucose in the composition (the amount of glucose (% by mass) in the composition before degradation) is measured, and then the glucan in the composition is degraded to glucose units and the amount of glucose in the composition after degradation (the amount of glucose (% by mass) in the composition after degradation) is measured. The amount of glucose can be measured, for example, by LC (liquid chromatography) analysis. The β-glucan content in the composition can be calculated from the measured glucose amount using the following formula: Glucan content (% by mass) = [Glucose amount (% by mass) in the composition after degradation - Glucose amount (% by mass) in the composition before degradation] × 0.9 Alternatively, the amount of β-glucan added as an activated carbon species generation promoter is preferably 5% by mass or more of the composition containing the treated yeast cell wall product, and may be 6% by mass or more, 7% by mass or more, 8% by mass or more, or 9% by mass or more. Furthermore, the upper limit of the amount added is approximately 60% by mass, and may be 55% by mass or less, 50% by mass or less, 45% by mass or less, or 40% by mass or less.

[0015] Treated yeast cell walls, such as hydrothermal reaction-treated yeast cell walls, contain iron (Fe) ions. The ability to generate activated carbon species can be improved by adding iron (Fe) ions or a compound capable of supplying iron (Fe) ions to the treated yeast cell walls as an activated carbon species generation promoter. For example, by adding iron (Fe) ions or a compound capable of supplying iron (Fe) ions as an activated carbon species generation promoter, the iron ion concentration of a composition containing a treated yeast cell wall product and having a β-glucan content of 4% by mass or more is preferably 10 mM or more, and may be 20 mM or more, 30 mM or more, or 40 mM or more. Furthermore, the iron (Fe) or iron (Fe) ion concentration of a composition containing a treated yeast cell wall product and having a β-glucan content of 4% by mass or more is preferably 10 mM or more, and may be 20 mM or more, 30 mM or more, or 40 mM or more. The amount of iron (Fe) ions in the composition can be analyzed by IPC emission spectroscopy or the like. Alternatively, the amount of iron (Fe) ions or a compound capable of supplying iron (Fe) ions added as an activated carbon species generation promoter is preferably 10 mM or more, and may be 20 mM or more, 30 mM or more, or 40 mM or more, relative to the composition containing the treated yeast cell wall product. Furthermore, the amount of divalent iron or trivalent iron ions added as an activated carbon species generation promoter is preferably 10 mM or more, and may be 20 mM or more, 30 mM or more, or 40 mM or more.

[0016] The composition containing the treated yeast cell wall product of the present invention can be prepared, for example, by incorporating the treated yeast cell wall product and an activated carbon species generation promoter into the composition. Furthermore, the activated carbon species generator of the present invention can be prepared, for example, by incorporating the treated yeast cell wall product and an activated carbon species generation promoter into the composition. For example, these can be prepared by mixing the hydrothermal reaction-treated yeast cell wall product with the activated carbon species generation promoter. Therefore, one aspect of the present invention relates to a method for producing an activated carbon species generator containing a treated yeast cell wall product, which includes mixing the treated yeast cell wall product with the activated carbon species generation promoter. The mixing method and the timing of mixing the hydrothermal reaction-treated yeast cell wall product with the activated carbon species generation promoter during the production process of the composition or the activated carbon species generator are not particularly limited and can be determined appropriately by those skilled in the art.

[0017] The composition of the present invention (i.e., a composition containing a treated yeast cell wall product containing 3 μmol / L or more of activated carbon species) or an activated carbon species generator can promote the death of cancer cells and / or inhibit the proliferation of cancer cells, and its mode of use is not particularly limited. For example, it can be directly contacted with an affected area containing cancer cells to kill or inhibit their proliferation. Furthermore, as long as it is possible to promote the death of cancer cells and / or inhibit their proliferation, a material may be interposed between the composition and the affected area. The action of the composition of the present invention (i.e., a composition containing a treated yeast cell wall product containing 3 μmol / L or more of activated carbon species) or an activated carbon species generator used as an anticancer composition can inhibit cancer cell adhesion, cytoskeleton formation, and induce apoptosis in cancer cells. As described above, the activated carbon species generator can generate activated carbon species that can pass through polystyrene walls (membranes (films)). Therefore, the anticancer composition of the present invention contains activated carbon species that have a polystyrene permeation effect. One aspect of the present invention provides a cancer treatment formulation or a cancer treatment device containing the composition of the present invention (i.e., a composition containing a treated yeast cell wall product containing 3 μmol / L or more of activated carbon species) or an activated carbon species generator. The dosage form of the cancer treatment formulation is not particularly limited, and can be, for example, an external preparation such as a patch. The specific form of the treatment device is also not particularly limited and can be appropriately determined by those skilled in the art. Another aspect of the present invention provides a cancer treatment formulation or a cancer treatment device that includes the composition of the present invention (i.e., a composition containing a treated yeast cell wall product containing 3 μmol / L or more of activated carbon species) or an activated carbon species generator and a container that contains the composition or activated carbon species generator. For cancer treatment, the exterior of the container may be brought into contact with an affected area containing a group of target cancer cells, for example. The material constituting the container is not particularly limited, as long as it is capable of promoting cancer cell death and / or inhibiting cancer cell proliferation through the action of the composition or activated carbon species generator in the container, and examples thereof include polyethylene, polystyrene, polyethylene terephthalate, and polypropylene.

[0018] As described above, the present invention provides a novel composition for treating cancer. Cancers that can be treated with the cancer treatment (anticancer) composition of this embodiment are not particularly limited, and examples include solid cancers such as breast cancer, cervical cancer, mesothelioma, small cell lung cancer, malignant melanoma, basal cell carcinoma, lymphoma, osteosarcoma, gastric cancer, and lung adenocarcinoma, and blood cancers such as acute leukemia.

[0019] [Preparation of a hydrothermal reaction product of yeast cell walls (hereinafter referred to as YCW-H (Yeast Cell Wall treated with Hydrothermal reaction))] After 143.6 g of distilled water was added to a magnetically stirred hydrothermal reaction vessel, 25.4 g of yeast cell walls (Asahi Group Foods Co., Ltd.) was added. The lid was closed and the contents were mixed by stirring, after which the temperature was raised. The contents were treated for 10 minutes under conditions of a pressure of 1.8 MPa and a temperature of 180°C to obtain YCW-H (a hydrothermal reaction product of yeast cell walls).

[0020] [Preparation of Comparative Composition] Distilled water was added to quartz porphyry powder (weight ratio of quartz porphyry powder to distilled water: 40:60), and gelation was performed in a wet ball mill (manufactured by Keiko Co., Ltd.). The resulting gelled quartz porphyry was mixed with YCW-H (weight ratio of quartz porphyry:YCW-H: 90:10) to obtain a comparative composition.

[0021] (Detection of RCS by ESR) To confirm the presence or absence of radical species in YCW-H, analysis was performed using electron spin resonance (ESR). A Bruker EMXplus (frequency: 9.44 GHz, output: 1 mW, X-band) was used as the instrument, and the measurement temperature was 20 K, the central magnetic field was 0.5050 T (5,050 G), the sweep magnetic field range was 1 T (10,000 G), the modulation magnetic field was 0.5 mT (5 G), and the time constant was 163.84 ms. Approximately 100 μL of YCW-H or a comparative composition was placed in a 5 mm diameter ESR quartz sample tube, rapidly cooled with liquid nitrogen, transferred to a low-temperature cavity, and measured at 20 K under a helium atmosphere. Win EPR (software supplied with Bruker) was used as the analysis software. A peak for reactive carbon species (hereinafter referred to as RCS (Reactive Carbon Species)) at g = 2.003 was observed in YCW-H (Figure 1A), indicating that RCS was generated from YCW-H. Furthermore, a peak for Fe(III)-complex at g = 4.25 was observed in YCW-H (Figure 1A), indicating that YCW-H contained Fe(III)-complex. Next, when Fe(II) (ferrous sulfate heptahydrate) or Fe(III) (ferric sulfate hydrate) was added to YCW-H to adjust the Fe concentration to 46.3 mM, the amount of RCS and the amount of Fe(III)-complex increased (Figures 1B-1D). The amount of Fe(III)-complex also increased with the addition of Fe(II). This suggests that the addition of Fe(II) may increase Fe(III). These results suggest that both Fe(II) and Fe(III) contribute to the RCS formation of YCW-H.

[0022] (Confirmation of RCS Passage Through Plastic Walls) To confirm the migration of radical species through plastic walls, electron spin resonance (ESR) analysis was performed. A Bruker EMXplus instrument (frequency: 9.44 GHz, output: 1 mW, X-band) was used. Measurements were performed at a measurement temperature of 20 K, a central magnetic field of 0.5050 T (5,050 G), a magnetic field sweep range of 1 T (10,000 G), a modulation magnetic field of 0.5 mT (5 G), and a time constant of 163.84 ms. Win EPR (software provided by Bruker) was used for analysis. Five mL of YCW-H was placed in a 15 mL polystyrene tube. A 5 mL polystyrene tube containing 0.5 mL of water containing the spin trapping agent N-tert-butyl-α-phenylnitrone (PBN) at a concentration of 20 mM was immersed in the solution for three days. After immersion, the PBN-added water in the 5 ml tube was subjected to ESR analysis. The ESR analysis results showed that the concentration of radical adducts with PBN increased from 0.43 μmol / L to 0.47 μmol / L. Furthermore, when Fe(III) (ferric sulfate hydrate) was added to YCW-H to adjust the Fe concentration to 46.3 mM, the concentration of radical adducts further increased to 0.51 μmol / L. These results suggest that radical species permeate the polystyrene wall, demonstrating their movement between completely separated compartments.

[0023] (Inhibition of proliferation of breast cancer MCF7 cells) MCF7 breast cancer cells were cultured in a 5% CO2 incubator at 37°C with YCW-H at a final concentration of 0-10% in medium (DMEM [Dulbecco's Modified Eagle's Medium] supplemented with 5% fetal bovine serum (FBS), 1% penicillin, and 1% streptomycin). The cells were then cultured for 5 days and counted. YCW-H inhibited the proliferation of MCF7 breast cancer cells in a dose-dependent manner, with an EC50 of 1.8% (Figure 2A). It is believed that RCS directly inhibited the proliferation of MCF7 breast cancer cells. Next, a proliferation assay (hereinafter referred to as "YCW-H adjacent treatment") was performed in a two-layer culture system in which a 3 cm inner dish was placed inside a 5 cm outer dish. YCW-H at concentrations ranging from 0% to 100% (diluted with distilled water) was filled into a 5-cm outer dish, and breast cancer cells MCF7 were cultured in a 3-cm inner dish (DMEM (Dulbecco's Modified Eagle's Medium) supplemented with 5% fetal bovine serum (FBS), 1% penicillin, and 1% streptomycin). A dose-dependent growth inhibition was observed, with an EC50 of 11.9% (Figure 2B). Next, a proliferation assay was performed using a triple-well dish (made of polystyrene) sealed with a cover glass. MCF7 breast cancer cells were cultured in three wells of the triple-well dish, and the outside of the culture wells was filled with a solution containing YCW-H or PBS (phosphate buffered saline) (Figure 2C). The "Close" in Figure 2D indicates that the culture wells were sealed with a cover glass, while the "Open" in Figure 2D indicates that the culture wells were not sealed with a cover glass. When YCW-H was loaded on the outside of the culture well, cell proliferation was strongly inhibited under "Open" conditions (Fig. 2E).

[0024] (Addition of iron increases RCS levels and improves breast cancer cell growth inhibitory effect, while addition of ethylenediaminetetraacetic acid (EDTA) reduces this effect.) The effects of adding iron and EDTA to YCW-H were evaluated. Adding Fe(II) or Fe(III) to YCW-H improved its growth inhibitory effect on breast cancer cells MCF7 (Figure 3A, adjacent treatment of YCW-H under open conditions). Next, adding EDTA to a mixture of Fe(III) and YCW-H reduced the RCS from 18 μmol / L to 9 μmol / L (Figure 3B). Furthermore, adding EDTA to YCW-H reduced its growth inhibitory effect on breast cancer cells MCF7 (Figure 3C). It is thought that RCS is generated when YCW-H and Fe react and bind, and that EDTA chelates Fe, inhibiting the binding between YCW-H and Fe, thereby suppressing the generation of RCS, resulting in a reduced growth inhibitory effect.

[0025] (Increase in RCS Amount and Inhibitory Effect on Breast Cancer Cell Proliferation by Addition of β-1,3-D-Glucan) When 5% by mass of β-1,3-D-glucan (derived from barley, Sigma) was added to YCW-H containing an RCS amount of 2.9 μmol / L (total glucan amount: 8.74% by mass), the RCS amount increased to 4.2 μmol / L. Furthermore, the inhibitory effect on the proliferation of breast cancer cells MCF7 was 77.9% for YCW-H containing the β-1,3-D-glucan, compared to 36.6% for the comparative composition (RCS amount: 0.3 μmol / L).

[0026] (Apoptosis Induction, Increase in Intracellular Reactive Oxygen Levels) The inhibition of breast cancer cell proliferation by YCW-H adjacent treatment was analyzed by flow cytometry using the ViaCount™ detection reagent to determine the percentage of live, dead, and apoptotic cells. Apoptosis is a highly regulated cell death process, whereas necrosis is caused by cytotoxicity. Apoptosis was observed in 25% of breast cancer cells compared with 11% in the control group. Overall, 50% of breast cancer cells died compared with 17% in the control group (Figure 4A). Furthermore, intracellular reactive oxygen species (ROS) levels are known to be closely related to apoptosis. We examined intracellular ROS levels following YCW-H adjacent treatment. While intracellular ROS levels were significantly elevated following YCW-H adjacent treatment, no increase in ROS levels was observed in the control group (Figure 4B).

Claims

1. A composition comprising a processed yeast cell wall containing activated carbon species at 3 μmol / L or more.

2. The composition according to claim 1, wherein the treated yeast cells are hydrothermally treated yeast cell walls.

3. The composition according to claim 1 or 2, further comprising an activated carbon species generation promoter.

4. The composition of claim 3, wherein the activated carbon species generation promoter increases the concentration of activated carbon species in the composition by at least about 1.4 times.

5. The composition according to claim 3 or 4, wherein the activated carbon species generation promoter comprises ferrous or ferric ions and / or β-glucan.

6. A composition according to any one of claims 1 to 5, having a β-glucan content of 4% by mass or more and a divalent iron or trivalent iron ion concentration of 10 mM or more.

7. The composition according to any one of claims 1 to 5, wherein the β-glucan content is 8% by mass or more.

8. The composition according to any one of claims 1 to 5, wherein β-glucan is added in an amount of 5% by mass or more.

9. An activated carbon species generator containing a yeast cell wall treatment.

10. The activated carbon species generator according to claim 9, which contains 0.20 mmol / L or more of an Fe(III) complex.

11. The activated carbon species generator according to claim 9 or 10, wherein the Fe(III)-complex concentration is increased by 1.3 times or more compared to the Fe(III)-complex concentration in the treated yeast cell wall.

12. The activated carbon species generator according to any one of claims 9 to 11, for generating activated carbon species having a polystyrene permeation effect.

13. An activated carbon species generation promoter for increasing the concentration of activated carbon species in treated yeast cell walls, comprising ferrous or ferric ions and / or β-glucan.

14. A method for producing an activated carbon species generator according to any one of claims 9 to 12, which comprises mixing a treated yeast cell wall product with an activated carbon species generation promoter.

15. A method for increasing the concentration of activated carbon species in a composition containing a treated yeast cell wall, comprising adding divalent iron or trivalent iron ions and / or β-glucan to the composition containing the treated yeast cell wall.

16. An anti-cancer composition containing the composition according to any one of claims 1 to 8 as an active ingredient.

17. The anti-cancer composition according to claim 16, which is used by directly contacting an affected area containing cancer cells.

18. An anti-cancer composition according to claim 16 or 17, comprising activated carbon species having a polystyrene permeation effect.

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