Composition for promoting hair growth or preventing hair loss, containing hemp stem extract as active ingredient

Abstract

The present invention relates to a composition for promoting hair growth or preventing hair loss, containing a hemp stem extract as an active ingredient. More specifically, the hemp stem extract of the present invention has an excellent effect of inducing proliferation of dermal papilla cells, and thus can be effectively used in a cosmetic composition, a quasi-drug, a pharmaceutical composition, or a health functional food composition for promoting hair growth, preventing hair loss, or promoting hair regrowth.

Description

Composition for promoting hair growth or preventing hair loss containing hemp stem extract as an active ingredient

[0001] The present invention relates to a composition for promoting hair growth or preventing hair loss, containing a hemp stem extract as an active ingredient.

[0002]

[0003] This research was supported by the Basic Research Project of the Korea Institute of Oriental Medicine, funded by the Ministry of Science and ICT (Project Number: KSN2412013).

[0004] The human body has approximately 100,000 to 150,000 hairs, and each hair undergoes a different hair cycle, repeating the growth phase (anagen), regression phase (catagen), and resting phase (telogen), generating, growing, and shedding hairs. As a result, approximately 15% of all hairs are in the resting and catagen phases, and an average of 50 to 100 hairs are normally shed and regenerated each day. This hair growth cycle repeats in cycles of approximately 3 to 6 years.

[0005] In general, hair loss refers to a condition in which the growth phase of the hair cycle becomes shorter due to various reasons, and the proportion of hair in the catagen and telogen phases increases relatively, resulting in more hair loss than average. Causes of hair loss include genetic androgen hormones, nutritional disorders due to local blood flow disorders, autonomic nervous system disorders, autoimmune diseases, and endocrine disorders, but the exact cause is still unknown. Finasteride, the most representative hair loss prevention drug, is a drug that inhibits the activity of 5α-reductase, which produces dihydrotestosterone (DHT), a substance that directly causes hair loss. However, finasteride can cause fetal malformations in pregnant women, and side effects such as decreased libido, erectile dysfunction, and ejaculation disorders have been reported with long-term use, so caution is required when taking it. Minoxidil-containing hair growth products, which are widely used, are also FDA-approved as over-the-counter drugs. However, they cannot be administered to women and have side effects such as skin irritation, itching, redness, and worsening hair loss. Therefore, there is a need to develop a substance that is safe for both men and women, has no side effects, and has hair growth and hair loss prevention effects.

[0006] The root of the hair follicle, from which hair grows, is called the hair bulb, and the hollow area beneath it is called the dermal papilla. The dermal papilla is the most important part of hair development. It contains blood vessels and nerves, and dermal papilla cells exist. They supply nutrients to the hair matrix cells located directly above them, promoting hair growth. The number of these dermal papilla cells is determined at birth, and as the dermal papilla cells disappear and their number decreases, the number of hairs also decreases.

[0007] Meanwhile, hemp, also called cannabis (Cannabis sativa L.), is an annual herbaceous plant belonging to the hemp family. The taproot extends 30-40 cm underground, but the lateral roots do not develop vigorously, so they are easily pulled out. Its height is about 3 m in temperate zones, but it grows up to 6 m in tropical regions. The stem is vertically grooved, and endophytic fibers are formed within the parenchyma, which are bast fibers. The leaves are palmate in shape, and are made up of 5-9 leaflets, which are opposite at the bottom of the stem and alternate at the top.

[0008] Prior art related to hair or hair loss includes Korean Patent No. 2295754, which discloses a "composition for strengthening hair roots, promoting hair growth, or preventing hair loss containing a soybean germ extract," and Korean Patent No. 1769402, which discloses a "composition for promoting hair growth or preventing hair loss containing a hemp stem extract as an active ingredient." However, the "composition for promoting hair growth or preventing hair loss containing a hemp stem extract as an active ingredient" of the present invention has not yet been disclosed.

[0009] The present invention was derived from the above-mentioned needs, and it was confirmed that the hemp stem extract, which is an effective ingredient of the present invention, has an excellent effect of inducing proliferation of dermal papilla cells, and it was confirmed that the hair regeneration rate and the rate of anagen hair follicles in hair-removed mice were increased compared to the untreated group, thereby completing the present invention.

[0010] In order to solve the above problem, the present invention provides a cosmetic composition for promoting hair growth or preventing hair loss containing a hemp stem extract as an active ingredient.

[0011] In addition, the present invention provides a pharmaceutical product for promoting hair growth or preventing hair loss containing a hemp stem extract as an active ingredient.

[0012] In addition, the present invention provides a pharmaceutical composition for preventing hair loss or promoting hair growth containing a hemp stem extract as an active ingredient.

[0013] In addition, the present invention provides a health functional food composition for promoting hair growth or preventing hair loss, containing a hemp stem extract as an active ingredient.

[0014] The present invention relates to a composition for promoting hair growth or preventing hair loss, which contains a hemp stem extract as an active ingredient. The hemp stem extract, which is an active ingredient of the present invention, has an excellent effect of inducing proliferation of dermal papilla cells and an excellent effect of promoting hair growth in hair-removed mice.

[0015] Figure 1 shows the results of confirming the effect of inducing proliferation of human hair follicle cells by treatment with hemp stem ethanol extract (A) or hemp stem hot water extract (B). **, ***, **** indicate that the proliferation of human hair follicle cells in the hemp stem ethanol extract or hemp stem hot water extract treatment group was statistically significantly increased compared to the untreated group; ** is p<0.01, *** is p<0.001, and **** is p<0.0001.

[0016] Figure 2 shows the results of examining the hair regeneration area ratio (A) and body weight change (B) after removing hair from the back of mice in the telogen hair growth cycle and orally administering hemp stem ethanol extract for 14 days. **, *** indicate that the hair regeneration area ratio of the hemp stem ethanol extract administration group or the 3% minoxidil administration group increased statistically significantly compared to the untreated group; ** is p<0.01, and *** is p<0.001.

[0017] Figure 3 shows the results of histological analysis of the skin of the area where hair grew on days 1, 7, and 14 after removing hair from the back of mice in the telogen hair growth cycle and orally administering hemp stem ethanol extract for 14 days, staining it with H&E, and confirming the ratio of hair follicles in the anagen phase on day 14. *** indicates that the ratio of hair follicles in the anagen phase increased statistically significantly in the hemp stem ethanol extract administration group or the 3% minoxidil administration group compared to the untreated group, p<0.001.

[0018] The present invention relates to a cosmetic composition for promoting hair growth or preventing hair loss, containing a hemp stem extract as an active ingredient.

[0019] The extraction solvent of the above hemp stem extract is preferably selected from among water, C1~C4 lower alcohol, or a mixture thereof, but is not limited thereto.

[0020] The hemp stem extract of the present invention may promote the growth of hair follicle cells, but is not limited thereto.

[0021] In addition, the cosmetic composition may be manufactured in the form of a hair tonic, a hair conditioner, a hair essence, a hair lotion, a hair nutrition lotion, a hair shampoo, a hair rinse, a hair treatment, a hair cream, a hair nutrition cream, a hair moisture cream, a hair massage cream, a hair wax, a hair aerosol, a hair pack, a hair nutrition pack, a hair soap, a hair cleansing foam, a hair drying agent, a hair preservation treatment agent, a hair dye, a hair waving agent, a hair bleaching agent, a hair gel, a hair glaze, a hair dressing agent, a hair lacquer, a hair moisturizer, a hair mousse, or a hair spray, but is not limited thereto.

[0022] In addition, the present invention relates to a pharmaceutical product for promoting hair growth or preventing hair loss containing a hemp stem extract as an active ingredient.

[0023] The over-the-counter drug for preventing hair loss or promoting hair growth of the present invention may be manufactured in the form of a cream, gel, patch, ointment, paste, lotion, liquid, emulsion, powder or spray, but is not limited thereto.

[0024] When the formulation of the present invention is a cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.

[0025] When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and particularly in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane / butane or dimethyl ether may be additionally included.

[0026] When the formulation of the present invention is a liquid or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

[0027] In addition, the present invention relates to a pharmaceutical composition for preventing hair loss or promoting hair growth containing a hemp stem extract as an active ingredient.

[0028] The pharmaceutical composition according to the present invention can be formulated and used in the form of oral formulations such as capsules, powders, granules, tablets, suspensions, emulsions, syrups, aerosols, external preparations, and sterile injection solutions, respectively, according to conventional methods.

[0029] The pharmaceutical composition according to the present invention may further comprise a pharmaceutically acceptable carrier, excipient or diluent.

[0030] Carriers, excipients and diluents that may be included in the pharmaceutical composition of the present invention include various compounds or mixtures including lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

[0031] When formulated, they are usually prepared using diluents or excipients such as fillers, bulking agents, binders, wetting agents, disintegrating agents, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, and capsules, and these solid preparations are prepared by mixing the pharmaceutical composition with at least one excipient, such as starch, calcium carbonate, sucrose or lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, oral solutions, emulsions, and syrups, and in addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, and suppositories. Non-aqueous solvents and suspending agents can include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. Suppository bases can include withepsol, macrogol, Tween 61, cocoa butter, laurin butter, and glycerogelatin.

[0032] The appropriate dosage of the pharmaceutical composition of the present invention can be prescribed in various ways depending on factors such as the formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate, and reaction sensitivity.

[0033] The pharmaceutical composition of the present invention can be administered orally or parenterally, and in the case of parenteral administration, it can be administered by topical application to the skin, intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc.

[0034] In addition, the present invention relates to a health functional food composition for promoting hair growth or preventing hair loss, which contains a hemp stem extract as an active ingredient.

[0035] The above health functional food composition may be manufactured in the form of powder, granules, pills, tablets, capsules, candy, syrup or beverage, but is not limited thereto.

[0036] When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as is or used together with other foods or food ingredients, and may be used appropriately according to a conventional method. The amount of the active ingredient may be appropriately used depending on the purpose of use (prevention or improvement). Generally, when manufacturing a food or beverage, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for health purposes, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount greater than the above range.

[0037] There are no specific restrictions on the types of the above health functional foods. Examples of foods to which the above health functional food composition can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea drinks, alcoholic beverages, and vitamin complexes, and include all health foods in the conventional sense.

[0038] In addition, the health functional food composition of the present invention can be manufactured into a food, particularly a functional food. The functional food of the present invention includes ingredients commonly added during food manufacturing, such as proteins, carbohydrates, fats, nutrients, and seasonings. For example, when manufactured into a drink, a natural carbohydrate or flavoring agent may be included as an additional ingredient in addition to the active ingredient. The natural carbohydrate is preferably a monosaccharide (e.g., glucose, fructose, etc.), a disaccharide (e.g., maltose, sucrose, etc.), an oligosaccharide, a polysaccharide (e.g., dextrin, cyclodextrin, etc.), or a sugar alcohol (e.g., xylitol, sorbitol, erythritol, etc.). The flavoring agent can be a natural flavoring agent (e.g., thaumatin, stevia extract, etc.) or a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).

[0039] In addition to the above health functional food composition, various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. may be further contained. The ratio of these added components is not particularly important, but is generally selected within the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health functional food composition of the present invention.

[0040]

[0041] Hereinafter, the present invention will be described in more detail using manufacturing examples and examples. It will be apparent to those skilled in the art that these manufacturing examples and examples are intended solely to illustrate the present invention more specifically and that the scope of the present invention is not limited by them.

[0042]

[0043] Example 1. Preparation of hemp stem extract

[0044] After crushing the dried hemp stems using a crusher, 3 L of 70% (v / v) ethanol was added to 1 kg of the crushed hemp stems and extracted by heating to 75°C. The extracted solution was filtered under reduced pressure through a Buchner funnel lined with filter paper after removing the solid content using a microfilter (0.45 μm), and the filtrate was concentrated under reduced pressure to obtain a hemp ethanol extract.

[0045] In addition, 10 times the amount of distilled water was added to the crushed hemp stem, hot water extraction was performed at 100°C for 180 minutes, and after the first filtering with 0.45 μm, the second filtering with 0.22 μm was performed to remove the precipitate, thereby obtaining a hemp hot water extract.

[0046] Afterwards, the hemp ethanol extract or hemp hot water extract was freeze-dried at -80°C and stored at -20°C for use in the experiment.

[0047]

[0048] Example 2. Effect of hemp stem extract on the proliferation of human hair follicle cells

[0049] Human dermal papilla cells were purchased from ABM (Applied Biological Materials, Canada). Human dermal papilla cells were seeded at 5 × 10 in a 48-well plate dish. 4After inoculating cells / well and culturing at 37°C for 2 days, the cells were treated with 0, 2.5, 5, 10, and 20 μg / mL of hemp stem ethanol extract or 0, 2.5, 5, and 10 μg / mL of hemp stem water extract, respectively, and cultured for 3 days. Afterwards, cell viability was measured using the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.

[0050] As a result, as disclosed in Fig. 1, it was confirmed that the proliferation of human hair follicle cells increased by treatment with hemp stem ethanol extract or hemp stem hot water extract. In particular, it was confirmed that the proliferation of human hair follicle cells increased in a concentration-dependent manner by treatment with hemp stem ethanol extract, and it was confirmed that 20 ㎍ / ㎖ of hemp stem ethanol extract increased the proliferation of human hair follicle cells by approximately 32.7% compared to the untreated group.

[0051]

[0052] Example 3. Effect of administration of hemp stem extract on hair growth promotion in mice.

[0053] An animal experiment was conducted to confirm the efficacy of hemp stem ethanol extract on promoting hair growth. Specifically, 6-week-old C57BL / 6 female mice in the telogen phase of the hair growth cycle were subjected to hair removal on the backs of mice in a 2 cm × 3.5 cm (width × height) area using a depilator and depilatory cream, and after a one-day rest period, the mice were orally administered 100 mg / kg or 300 mg / kg of hemp stem ethanol extract suspended in saline solution daily for 14 days. An untreated group was used as a control group, and 3% (v / v) minoxidil, a hair loss treatment, was orally administered instead of the hemp stem ethanol extract and used as a positive control group.

[0054]

[0055] 3-1. Analysis of hair regeneration area ratio

[0056] After oral administration of hemp stem ethanol extract, body weight was measured 14 days later, and the hair growth condition was visually confirmed. Using the Image J program, the total area of ​​the mouse and the area where hair was regenerated were measured, and the hair regeneration area ratio (%) was calculated by substituting the results into the equation below.

[0057] Hair regeneration area ratio (%) = [Hair regeneration area / Total mouse area] × 100

[0058] As a result, as disclosed in Fig. 2A, it was confirmed that the hair regeneration area ratio of the hemp stem ethanol extract administration group significantly increased compared to the untreated group. In particular, the hair regeneration area ratio of the 100 mg / kg hemp stem ethanol extract administration group increased by approximately 55.2% compared to the untreated group, the hair regeneration area ratio of the 300 mg / kg hemp stem ethanol extract administration group increased by approximately 83.3% compared to the untreated group, and the hair regeneration area ratio of the 3% (v / v) minoxidil treatment group increased by approximately 74.0% compared to the untreated group.

[0059] Additionally, as disclosed in Figure 2B, it was confirmed that there was almost no change in the body weight of mice due to administration of hemp stem ethanol extract.

[0060]

[0061] 3-2. Analysis of the proportion of hair follicles in the growth phase (anagen)

[0062] For histological analysis, the skin of the area where hair regenerated was removed 1, 7, and 14 days after oral administration of hemp stem ethanol extract, fixed with formalin, dehydrated stepwise and deparaffinized, stained with H&E (Hematoxylin & Eosin), and observed for changes in hair follicle tissue under an optical microscope.

[0063] As a result, as disclosed in Fig. 3, it was confirmed that the number, size, and depth of hair follicles in the scalp increased compared to the untreated group by administration of hemp stem ethanol extract. In particular, on the 14th day, the proportion of hair follicles in the anagen phase in the group administered 100 mg / kg of hemp stem ethanol extract increased by approximately 23.5% compared to the untreated group, and the proportion of hair follicles in the anagen phase in the group administered 300 mg / kg of hemp stem ethanol extract increased by approximately 64.7% compared to the untreated group.

[0064]

[0065] Based on the above results, it was found that the hemp stem extract of the present invention has an excellent effect of inducing proliferation of hair follicle cells, and exhibits hair growth promotion efficacy similar to that of minoxidil, a hair loss treatment agent, while exhibiting no toxicity.

Claims

1. A cosmetic composition for promoting hair growth or preventing hair loss containing hemp stem extract as an active ingredient.

2. A cosmetic composition for promoting hair growth or preventing hair loss, characterized in that the extraction solvent of the hemp stem extract in paragraph 1 is water, a lower alcohol of C1 to C4, or a mixture thereof.

3. A cosmetic composition for promoting hair growth or preventing hair loss, characterized in that the hemp stem extract in paragraph 1 promotes the growth of hair papilla cells.

4. A cosmetic composition for promoting hair growth or preventing hair loss, characterized in that the composition in claim 1 is manufactured in the form of a hair tonic, a hair conditioner, a hair essence, a hair lotion, a hair nutrition lotion, a hair shampoo, a hair rinse, a hair treatment, a hair cream, a hair nutrition cream, a hair moisture cream, a hair massage cream, a hair wax, a hair aerosol, a hair pack, a hair nutrition pack, a hair soap, a hair cleansing foam, a hair drying agent, a hair preservation treatment agent, a hair dye, a hair waving agent, a hair bleaching agent, a hair gel, a hair glaze, a hair dressing agent, a hair moisturizer, a hair mousse or a hair spray.

5. A non-prescription drug for promoting hair growth or preventing hair loss containing hemp stem extract as an active ingredient.

6. A non-prescription drug for promoting hair growth or preventing hair loss, characterized in that the non-prescription drug in the 5th paragraph is manufactured in the form of a cream, gel, patch, ointment, warning agent, lotion, liquid, emulsion, powder or spray.

7. A pharmaceutical composition for preventing hair loss or promoting hair growth containing hemp stem extract as an active ingredient.

8. A pharmaceutical composition for preventing hair loss or promoting hair growth, characterized in that the composition is prepared in any one formulation selected from among capsules, powders, granules, tablets, suspensions, emulsions, syrups, and aerosols in accordance with paragraph 7.

9. A health functional food composition for promoting hair growth or preventing hair loss containing hemp stem extract as an active ingredient.

10. A health functional food composition for promoting hair growth or preventing hair loss, characterized in that the composition is manufactured in any one formulation selected from powder, granules, pills, tablets, capsules, candy, syrup, and beverage in the 9th paragraph.

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