Detection of bacterial extracellular membrane vesicles in probiotic bacteria-based biofilm / food packaging

Abstract

The invention relates to a new composite formed with Prebiotic xylooligosaccharide, Probiotic Bifidobacterium Infantis and Postbiotic Sodium Alginate and Chitre, Bicarbonate, Glycerol, a powder form composite with unique properties and applications, after the production of probiotic bacteria-based food packaging, the extracellular membrane vesicles of the bacteria in the biofilm in solid form turning into live probiotic bacteria after the biofilm is dissolved in water.

Description

[0001] SPECIFICATION

[0002] DETECTION OF BACTERIAL EXTRACELLULAR MEMBRANE VESICLES IN PROBIOTIC BACTERIA-BASED BIOFILM / FOOD PACKAGING

[0003] Technical Field

[0004] The invention relates to the study of bacterial extracellular membrane vesicles in solid and dissolved form in biofilm / food packaging, in biodegradable material based on probiotic bacteria that can be used in the health, agriculture and food sectors, as well as reducing the carbon content and postbiotic characteristic bacterial extracellular membrane vesicles with medical support therapeutic properties.

[0005] Background of the Invention

[0006] In a world that is increasingly focused on sustainability, the intersection of biology and materials science is bringing about a remarkable innovation: Probiotic Bacteria- Based Biomaterials, combining the benefits of probiotic bacteria with biodegradable polymers to pave the way for a sustainable, environmentally friendly future.

[0007] At the same time, materials that benefit the environment are materials that are biodegradable biomaterials based on probiotic bacteria. At the heart of this technology, there is a synergy between beneficial probiotic microorganisms and biodegradable polymers such as starch and cellulose.

[0008] The production journey of these innovative materials starts with the careful selection of strains of probiotic bacteria that are compatible with biodegradable polymers. These strains are cultivated in sterile fermentation environments under controlled conditions of temperature, pH and oxygen levels. As they grow, they produce metabolites that enhance the properties of biodegradable polymers.

[0009] When the probiotic cultures reach the desired state, they are incorporated into the biodegradable polymer matrix, thus ensuring even distribution and functional improvement. Processing techniques such as injection molding are used to shape the final biomaterials. Probiotic bacteria-based biomaterials offer numerous advantages:

[0010] Biodegradability: These materials biodegrade, reducing the plastic waste load in landfills and oceans.

[0011] Functional Properties: Probiotic metabolites enhance material properties, making them suitable for a variety of applications.

[0012] Sustainability: The use of renewable polymers and probiotics promotes environmentally responsible production.

[0013] Health Benefits: Potential applications range from the creation of biofunctional materials that support human and environmental health. In Probiotic Bacteria-based biodegradable organic biofilm / food packaging, the potential presence of bacterial extracellular membrane vesicles of postbiotic character with supportive therapeutic properties, not only as packaging materials, but also as supplements, can lead to positive effects in the gut and support immune enhancement.

[0014] Frequent quality control and testing procedures ensure the viability, biodegradability and desired material properties of probiotic cultures. Microbiological analysis, biodegradability and mechanistic evaluations are essential components of the quality assurance process.

[0015] Probiotic Bacteria-Based Biomaterials represent an important step forward in the quest for a sustainable and environmentally friendly future. These materials have the potential to revolutionize industries from packaging to healthcare by offering a viable alternative to traditional plastics.

[0016] For a greener world, innovations in the production of biomaterials showcase the extraordinary possibilities that arise when harnessing the wisdom of nature for the betterment of society and the planet. Probiotic bacteria-based materials are not just a technological advance; they are a testament to the harmonious coexistence of science and nature, paving the way for a sustainable and healthier future for all.

[0017] Currently used petroleum-derived food packaging has a negative impact on the environment and human health. The collection and recycling of these products is a major problem in terms of time and cost.

[0018] The main advantages of biobased products lie in their sustainability, lower carbon footprint, biodegradability, reduced toxicity and gut / immune-boosting potential. By using renewable resources, biobased products contribute to the protection of ecosystems and minimize the depletion of scarce resources. Their production also tends to emit fewer greenhouse gases and thus has a positive impact on climate change.

[0019] The invention is based on making the probiotic bacteria-based polymer material to be produced with organic saccharide structures (starch, cellulose, etc.) into a biodegradable material and microscopic examination of the solid and water-soluble forms of the biofilm obtained with immersion oil.

[0020] The structural and characteristic features and all advantages of the invention will be clearly understood in the detailed description below, and therefore, the evaluation of the invention should be made in view of the detailed description.

[0021] Detailed Description of the Invention

[0022] In this detailed description, the invention is described in such a way that it has no limiting effect on the production of probiotic bacteria-based biomaterials.

[0023] The invention combines Probiotic Bifidobacterium Infantis, Xylooligosaccharide and Sodium Alginate with a material known as “Prebioticxylooligosaccharide, Probiotic Bifidobacterium Infantis and Postbiotic Sodium Alginate”, which is known as “Probiotic Biomposite (Probif)”, and organic material such as Chitre, Glycerin and Bicarbona. The components used in the production of the invention probiotic bacteria-based biomaterial are as follows; into the (1OOgr) material by weight;

[0024] • A new composite (in powder form) formed with Prebiotic xylooligosaccharide, Probiotic Bifidobacterium Infantis and Postbiotic Sodium Alginate in the range of 1-30%

[0025] • Bicarbonate in powder form in the range of 1 -40 %

[0026] • Chitre in powder form in the range of 1 -20%

[0027] • Glycerol in the range of 1-10% (in powder form) Biofilm is obtained after stirring the solution in a magnetic stirrer at 600 rpm for 90 minutes.

[0028] When the solid form of the biofilm obtained was examined under conventional microscopy with immersion oil at 10- 40-100 magnification, bacterial vesicles with a double membrane structure were detected, some of the vesicles tended to merge and were shown for the first time.

[0029] The solid biofilm structure was dissolved in 250 ml distilled water and when the sample was taken, it was determined that the probiotic Bifidobacterium Infantis strain in liquid form was alive and in branched chains.

[0030] The basis of the invention is a novel composite composed of Prebiotic xylooligosaccharide, Probiotic Bifidobacterium Infantis and Postbiotic Sodium Alginate, a mixture of Probiotic Bifidobacterium Infantis, Xylooligosaccharide and Sodium Alginate. This composition gives the material not only biodegradability but also postbiotic benefits with Probiotic and bacterial extracellular membrane vesicles, making it suitable for health and environmentally sensitive applications.

[0031] The glycerol in powder form is included in the material. Beyond the plasticizer role, it contributes to the flexibility and strength of the material.

[0032] Powdered Bicarbonate and Chitre contribute to the strength and flexibility of the biofilm formed.

[0033] A new composite Additive created with Prebiotic xylooligosaccharide, Probiotic Bifidobacterium Infantis and Postbiotic Sodium Alginate: A new composite composed of powdered Prebiotic xylooligosaccharide, Probiotic Bifidobacterium Infantis and Postbiotic Sodium Alginate is carefully mixed into the formulation. Varying concentrations offer flexibility in adjusting the probiotic content of the material.

[0034] Glycerol Increase: Glycerol in the form of powder is added to the blend. Incorporation contributes to the flexibility and strength of the material. Environmentally Friendly Packaging: Focusing on biodegradability, it is ideal for storage bags and reduces environmental impact.

[0035] Advances in Healthcare Services: The presence of bacterial extracellular membrane vesicles in Probiotic Bacteria-based biodegradable organic biofilm / food packaging, which have postbiotic properties in solid form, causes positive effects in the intestine and supports immune strengthening not only as packaging materials but also with their reinforcing properties. When the biofilm in which these vesicles are located is dissolved with water and similar liquids, the vesicles become active, alive and branched-chain, and probiotic bacteria can emerge and multiply in the intestine. When the packaging is disposed of in nature, it will make an important contribution to ecological sustainability with positive effects such as increasing the diversity of flora in places such as soil and sea, and destroying microplastics in soil and water.

[0036] By blending probiotic-containing biomaterials and precise production techniques, it is at the forefront of sustainable materialization. This invention not only offers an environmentally conscious alternative to conventional plastics, but also offers probiotic benefits to various industries, paving the way for a more sustainable, healthier future for both the environment and humanity.

[0037] All technical and other features mentioned in each claim are followed by a reference number and these reference numbers are used only to facilitate understanding of the claims, so they should not be regarded as limiting the scope of any of the items mentioned by these reference numbers for the purposes of illustration.

[0038] It is obvious that a person skilled in the art can also reveal the innovation in the invention by using similar embodiments and / or can apply this embodiment to other areas of similar purpose used in the relevant art. Therefore, it is obvious that such embodiments will lack the criterion of novelty and especially the criterion of exceeding the state of the art.

Claims

CLAIMSProbiotic bacteria-based biomaterial, wherein it comprises; (100gr)• A new composite formed with Prebiotic xylooligosaccharide, Probiotic Bifidobacterium Infantis and Postbiotic Sodium Alginate in the range of 1-30% (in powder form)• Bicarbonate in powder form in the range of 1 -40 %• Chitre in powder form in the range of 1 -20%• Glycerol in the range of 1-10% (in powder form)1. Probiotic bacteria-based biomaterial according to Claim 1 , wherein it contains a novel composite created with a Prebiotic xylooligosaccharide, Probiotic Bifidobacterium infantis and Postbiotic Sodium Alginate comprising Probiotic Bifidobacterium infantis, Xylooligosaccharide and Sodium alginate in a ratio optimized for probiotic activity and biodegradability.

2. Probiotic bacteria-based biomaterial according to Claim 1 , wherein it contains Chitre, Bicarbonate, Glycerol Monostearate which contribute to the flexibility and elasticity of the bioplastic material.

3. The production of probiotic bacteria-based food packaging; wherein• A new composite of prebiotic xylooligosaccharide, probiotic Bifidobacterium Infantis and postbiotic Sodium Alginate is incorporated in powder form and powdered Chitre, Bicarbonate Glycerol Monostearate is added,• A new composite formed with Prebiotic xylooligosaccharide, Probiotic Bifidobacterium Infantis and Postbiotic Sodium Alginate is blended with Chitre, Glycerol, Bicarbonate and mixed at 600 rpm in a magnetic mixer for 90 minutes. The solid form of the biofilm obtained is based on the detection of bacterial vesicles in double membrane structure and the tendency of some of these vesicles to merge when examined at 10-40-100 magnification in conventional Microscopy with immersion oil, Solid biofilm structure is based on the detection of Probiotic Bifidobacterium Infantis strain in liquid form in the form of live and branched chain when dissolved in 250 ml distilled water and sampled.