Composition comprising astragalus membranaceus root extract for soothing, repairing, and moisturizing skin

Abstract

A composition comprising an Astragalus membranaceus root extract for soothing, repairing, and moisturizing skin. The composition comprises an Astragalus membranaceus root extract, glycerin, methyl propylene glycol, a soothing and repairing ingredient, an antioxidant, a thickener, and water, wherein in the cosmetic composition, the weight percentage of the Astragalus membranaceus root extract is 1%-2%. The composition has excellent soothing, repairing, and moisturizing functions, can improve the skin barrier function, and can effectively alleviate the sensitive skin problem.

Description

A composition containing Astragalus membranaceus root extract for soothing, repairing and moisturizing skin

[0001] Cross-reference to Related Applications

[0002] The present application is based on and claims priority to the application with CN application number 202411158230.0 and filing date of August 22, 2024, the contents of which are hereby incorporated by reference in their entirety. TECHNICAL FIELD

[0003] The present application belongs to the field of cosmetics, especially skin care products, and specifically relates to a composition containing Astragalus membranaceus root extract for soothing, repairing and moisturizing skin. BACKGROUND

[0004] In recent years, due to factors such as climate change, environmental pollution, social pressure, and the complexity of cosmetic types and ingredients, the incidence of sensitive skin is high, about 36%, and the prevention and relief of symptoms of sensitive skin are in urgent need of solutions. Sensitive skin is a high reaction state of the skin under physiological or pathological conditions, which is manifested as easy to appear burning, stinging, itching and tightness when stimulated by physical, chemical, mental and other factors. The causes of sensitive skin are mainly three: impaired skin barrier function, damaged nervous system, and inflammatory response. These three mechanisms of action all lead to impaired skin barrier function, so enhancing the skin barrier function can effectively relieve the symptoms of sensitive skin. Given the structural basis of the skin barrier, natural moisturizing factor, the skin barrier can be enhanced by focusing on moisturizing and hydrating. Assisted by microbial flora to protect the skin barrier, reduce inflammation and improve skin health, and then solve the problem of sensitive skin. There are many skin care products on the market with soothing, repairing and moisturizing effects, but the soothing, repairing and moisturizing ability of existing soothing, repairing and moisturizing skin care products is limited, and they cannot effectively relieve the problem of sensitive skin.

[0005] SUMMARY

[0006] In order to solve the problems existing in the prior art, the present application provides a cosmetic composition which has excellent soothing, repairing and moisturizing functions, can improve the skin barrier function, and effectively relieve the problem of sensitive skin.

[0007] Therefore, in the first aspect of the present application, the present application provides a cosmetic composition comprising:

[0008] Astragalus membranaceus root extract, glycerin, methylpropanediol, soothing and repairing ingredients, antioxidants, thickening agents and water, wherein the weight percentage of the Astragalus membranaceus root extract in the cosmetic composition is 1-2%.

[0009] In some embodiments, the cosmetic composition comprises (by weight percentage):

[0010] 1-10% (e.g., 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10%) of a soothing repair ingredient,

[0011] 0.1-10% (e.g., 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10%) of glycerin,

[0012] 1-10% (e.g., 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10%) of methylpropanediol,

[0013] 1-20% (e.g., 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, or 20%) of a soothing repair ingredient,

[0014] 0.01-1% (e.g., 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 1%) of an antioxidant,

[0015] 0.01-1% (e.g., 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 1%) of a thickening agent, and

[0016] a balance of water.

[0017] In some embodiments, the soothing repair ingredient is selected from a microbial fermentation product, a plant extract, an algal extract, a plant oil, or any combination thereof.

[0018] In some embodiments, the plant oil is present in the cosmetic composition in an amount of 0.1-0.3% by weight.

[0019] In some embodiments, the cosmetic composition further has one or more technical features selected from (i)-(iv) below:

[0020] (i) the microbial fermentation product is a Lactobacillus / soy milk fermentation product filtrate;

[0021] (ii) the plant extract is a Piper cubeba root extract;

[0022] (iii) the algal extract is a Phaeodactylum tricornutum extract;

[0023] (iv) the plant oil is a mixed lavandula oil.

[0024] In some embodiments, the cosmetic composition comprises (by weight percent):

[0025] 1-2% Membrane podocarpus roots extract,

[0026] 0.1-10% (such as 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10%) glycerin,

[0027] 1-10% (such as 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10%) methylpropanediol,

[0028] 1-5% (such as 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5%) lactobacillus / soybean ferment filtrate,

[0029] 1-5% (such as 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5%) kava root extract,

[0030] 1-5% (such as 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5%) phaeophycea extract,

[0031] 0.1-0.3% (such as 0.1%, 0.2%, or 0.3%) lavandula hybrida oil,

[0032] 0.01-1% (such as 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 1%) antioxidant,

[0033] 0.01-1% (such as 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 1%) thickening agent, and

[0034] balance water.

[0035] In some embodiments, the cosmetic composition comprises (by weight percent):

[0036] 1-2% Membrane podocarpus roots extract,

[0037] 1-3% glycerin,

[0038] 5-7% methylpropanediol,

[0039] 1-3% lactobacillus / soybean ferment filtrate,

[0040] 1-2% kava root extract,

[0041] 1-3% of Phaeodactylum tricornutum extract,

[0042] 0.1-0.3% of Lavandula hybrida oil,

[0043] 0.4-0.6% of antioxidant,

[0044] 0.1-0.3% of thickening agent, and

[0045] balance water.

[0046] In some embodiments, the cosmetic composition (by weight percent) comprises:

[0047] 2% of Astragalus membranaceus root extract,

[0048] 2% of glycerin,

[0049] 6% of methylpropanediol,

[0050] 2% of Lactobacillus ferment lysate,

[0051] 1.5% of Piper methysticum root extract,

[0052] 2% of Phaeodactylum tricornutum extract,

[0053] 0.1% of Lavandula hybrida oil,

[0054] 0.5% of antioxidant,

[0055] 0.2% of thickening agent, and

[0056] balance water;

[0057] Alternatively,

[0058] the cosmetic composition (by weight percent) comprises:

[0059] 1% of Astragalus membranaceus root extract,

[0060] 2% of glycerin,

[0061] 6% of methylpropanediol,

[0062] 2% of Lactobacillus ferment lysate,

[0063] 1.5% of Piper methysticum root extract,

[0064] 2% of Phaeodactylum tricornutum extract,

[0065] 0.1% of Lavandula hybrida oil,

[0066] 0.5% of antioxidant,

[0067] 0.2% of a thickening agent, and

[0068] balance water;

[0069] Alternatively,

[0070] The cosmetic composition (by weight percent) comprises:

[0071] 2% of a Membranae Radix Astragali Extract,

[0072] 2% of glycerin,

[0073] 6% of methylpropanediol,

[0074] 2% of a Lactobacillus Ferment Filtrate,

[0075] 1.5% of a Piper Methysticum Root Extract,

[0076] 2% of a Phaeophyceae Laminariales Extract,

[0077] 0.2% of a Lavandula Hybrida Oil,

[0078] 0.5% of an antioxidant,

[0079] 0.2% of a thickening agent, and

[0080] balance water;

[0081] Alternatively,

[0082] The cosmetic composition (by weight percent) comprises:

[0083] 2% of a Membranae Radix Astragali Extract,

[0084] 2% of glycerin,

[0085] 6% of methylpropanediol,

[0086] 2% of a Lactobacillus Ferment Filtrate,

[0087] 1.5% of a Piper Methysticum Root Extract,

[0088] 2% of a Phaeophyceae Laminariales Extract,

[0089] 0.3% of a Lavandula Hybrida Oil,

[0090] 0.5% of an antioxidant,

[0091] 0.2% of a thickening agent, and

[0092] balance water.

[0093] In some embodiments, the cosmetic composition has one or more technical features selected from (i)-(ii) below:

[0094] (i) the antioxidant is selected from the group consisting of butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), tertiary butylhydroquinone (TBHQ), propyl gallate, rubenite, para-hydroxyacetophenone, or any combination thereof;

[0095] Preferably, the antioxidant is para-hydroxyacetophenone.

[0096] (ii) the thickening agent is selected from the group consisting of xanthan gum, magnesium aluminum silicate, agar, carbomer, sodium alginate, or any combination thereof;

[0097] Preferably, the thickening agent is xanthan gum.

[0098] In some embodiments, the Astragalus membranaceus root extract is obtained by the following steps:

[0099] Boil Astragalus membranaceus in water twice, the first time for 1-10 hours (such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 hours, preferably 3 hours), and the second time for 1-10 hours (such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 hours, preferably 2 hours), and combine the decoctions; filter the combined decoction to obtain a filtrate; concentrate the filtrate, and add ethanol to a content of 60-80% (such as 60%, 65%, 70%, 75%, or 80%, preferably 70%), stir well, and stand still; recover the ethanol from the supernatant, and concentrate to a clear extract with a relative density of 1.31-1.33 (measured at 60°C); dry and pulverize to obtain the Astragalus membranaceus root extract.

[0100] As understood by one skilled in the art, “boil” means to bring to a boil after adding water to Astragalus membranaceus.

[0101] In some embodiments, the Lactobacillus / soybean milk fermentation product filtrate is obtained by the following steps: immerse soybeans in water, then grind, heat, and press to obtain soy milk; sterilize the soy milk, inoculate with a lactobacillus strain, and ferment at 35-40°C (such as 35°C, 36°C, 37°C, 38°C, 39°C, or 40°C, preferably 37°C) for 100-150 hours (such as 100, 110, 120, 130, 140, or 150 hours, preferably 120 hours) to obtain a non-diluted fermentation broth, which is sterilized and filtered to obtain the Lactobacillus / soybean milk fermentation product filtrate.

[0102] In some embodiments, the Piper methysticum root extract is obtained by the following steps: take the roots of Piper methysticum, cold soak with water and butylene glycol, and filter to obtain the Piper methysticum root extract.

[0103] As understood by those skilled in the art, "cold soak" refers to a soaking process carried out at room temperature or a lower temperature, which is usually used to extract effective components in cosmetics and is a commonly used mild extraction method that can avoid component damage or deterioration caused by high temperature.

[0104] In some embodiments, the Phaeodactylum tricornutum extract is obtained by culturing Phaeodactylum tricornutum in a closed photobioreactor, and then sequentially purifying, CO2 supercritical extraction, and diluting.

[0105] As understood by those skilled in the art, "purification" during the preparation of cosmetics mainly refers to removing or reducing microorganisms, impurities, and pollutants in the product during the production process through a series of measures to ensure the quality and safety of the product.

[0106] In some embodiments, the lavender oil is obtained by steam distillation of the inflorescences of lavender (a hybrid mixture of lavender and spike lavender).

[0107] In some embodiments, the cosmetic composition is a skin care product composition.

[0108] In a second aspect of the present application, the present application provides a cosmetic comprising the cosmetic composition of any of the technical solutions of the first aspect.

[0109] In some embodiments, the cosmetic is a skin care product.

[0110] In a third aspect of the present application, the present application provides a method for preparing the cosmetic composition of any of the technical solutions of the first aspect, which comprises:

[0111] Disperse the Astragalus membranaceus root extract, glycerol, and thickening agent in water, heat to 80-85°C (such as 80°C, 81°C, 82°C, 83°C, 84°C, or 85°C, preferably 83°C), and perform first mixing; after uniform mixing, cool to 30-50°C (30°C, 35°C, 40°C, 45°C, or 50°C, preferably 40°C) to obtain phase A;

[0112] Perform second mixing of the antioxidant and methylpropanediol, heat to 60-65°C (such as 60°C, 61°C, 62°C, 63°C, 64°C, or 65°C, preferably 60°C), and after uniform mixing, cool to 30-50°C (30°C, 35°C, 40°C, 45°C, or 50°C, preferably 40°C) to obtain phase B;

[0113] Perform third mixing of the soothing and repairing ingredients, and after uniform mixing, obtain phase C;

[0114] adding the B phase and the C phase into the A phase, performing a fourth mixing, and obtaining the cosmetic composition after mixing uniformly;

[0115] Preferably, the fourth mixing is performed at a stirring speed of 400-600 r / min (such as 400 r / min, 450 r / min, 500 r / min, 550 r / min or 600 r / min).

[0116] Preferably, the fourth mixing is performed for 20-30 min (such as 20 min, 25 min or 30 min).

[0117] In some embodiments, the weight percentages of the Astragalus membranaceus (Fisch.) Bunge root extract, the glycerin, the methylpropanediol, the soothing and repairing ingredient, the antioxidant, and the thickening agent are as described in any of the technical solutions of the first aspect. Beneficial effects

[0118] (1) In terms of improving skin barrier function, the present application utilizes Astragalus membranaceus (Fisch.) Bunge root extract containing astragalus polysaccharides. Polysaccharides have good film-forming properties, contain a large number of hydrophilic groups in their molecules, and have good water absorption and water retention properties through hydrogen bonding due to the presence of hydroxyl groups in the structure of astragalus polysaccharides. Polar groups such as carboxyl groups can also bind to water molecules through hydrogen bonds, increasing skin moisture and improving the moisturizing properties of the stratum corneum and skin barrier function. In combination with polyols (glycerin and methylpropanediol), the moisturizing properties can be synergistically enhanced. Lactobacillus / soybean milk fermentation product filtrate is beneficial for skin conditioning, increasing the abundance of resident skin bacteria, reducing the abundance of pathogenic bacteria such as Escherichia coli and Vibrio cholerae, and maintaining skin microecological balance, thereby improving skin barrier function.

[0119] (2) The experimental results show that Astragalus membranaceus (Fisch.) Bunge root extract not only acts as a moisturizing agent to provide moisturizing effects, but also has obvious effects on skin soothing and repairing.

[0120] (3) The present application achieves soothing effects through aromatherapy. Linalyl acetate in the lavender oil has a direct sedative effect on the central nervous system, and also has a nerve balance restoring effect, an anti-inflammatory effect, and a blood pressure lowering effect. Linalool has a sedative, anti-anxiety effect, and an immune regulating effect. In addition, experiments have found that the lavender oil (also known as lavender essential oil, which will not be described in detail below) not only has a soothing effect, but also has a repairing effect on the skin, especially a moisturizing effect.

[0121] (4) The inventors have found that when the Astragalus membranaceus (Fisch.) Bunge root extract in the skin care composition of the present application is within a specific weight ratio range (such as 1%-2%), the moisturizing, soothing and repairing effects are significantly improved, which are significantly better than other weight ratios.

[0122] (5) The inventors found that, in the skin care composition of the present application, the heterogenous lavender oil, when in a specific weight ratio range (e.g. 0.1%-0.3%), not only significantly improves the soothing and repairing effects, but also significantly improves the moisturizing effect, and is significantly superior to other weight ratios. DETAILED DESCRIPTION

[0123] The embodiments of the present application will be described in detail below with reference to the examples, but those skilled in the art will understand that the following examples are only used to illustrate the present application, and are not a limitation on the scope of the present application. According to the following detailed description of the preferred embodiments, various objects and advantages of the present application will become apparent to those skilled in the art.

[0124] In the present application, the amount of each component (%) refers to the weight percentage of each component in the total weight of the skin care composition.

[0125] The raw materials for preparing the composition in the examples belong to self-developed production or are commercially available, and the relevant information is as follows:

[0126] The Astragalus membranaceus Bge. root extract is obtained by the following steps: decocting Astragalus membranaceus Bge. twice with water, the first time for 3 hours and the second time for 2 hours, and combining the decoction; filtering the decoction to obtain a filtrate; concentrating the filtrate and adding ethanol to make the ethanol content 70%, stirring uniformly, and standing; recovering ethanol from the supernatant, concentrating into a clear paste with a relative density of 1.31-1.33 (measured at 60°C), drying and crushing to obtain the Astragalus membranaceus Bge. root extract.

[0127] The Lactobacillus / soybean milk fermentation product filtrate is obtained by the following steps: immersing soybeans in water, then grinding, heating, and pressing to obtain soy milk, sterilizing the soy milk, inoculating with a lactobacillus strain, and fermenting at 37°C for 120 hours to obtain an undiluted fermentation broth, and filtering to obtain the Lactobacillus / soybean milk fermentation product filtrate.

[0128] The Piper methysticum root extract is obtained by the following steps: taking Piper methysticum roots, cold-soaking with water and butanediol, and filtering to obtain the Piper methysticum root extract.

[0129] The Phaeodactylum tricornutum extract is obtained by the following steps: culturing Phaeodactylum tricornutum in a closed photobioreactor, and sequentially treating by purification, CO2 supercritical extraction, and dilution to obtain the Phaeodactylum tricornutum extract.

[0130] The heterogenous lavender oil is obtained by the following steps: taking the inflorescences of heterogenous lavender (a hybrid mixture of lavender and spike lavender), and distilling with water vapor to obtain the heterogenous lavender oil.

[0131] The present application will be further explained and described below with reference to specific examples.

[0132] Example 1-4: Preparation of skin care composition

[0133] Take Astragalus membranaceus root extract, glycerin, xanthan gum and disperse them in water, stir, heat to 83°C, mix evenly, and then cool to 40°C to obtain phase A. Take p-hydroxyacetophenone, methylpropanediol, stir, heat to 60°C, mix evenly, and then cool to 40°C to obtain phase B. Take Lactobacillus / soybean milk fermentation product filtrate, kava root extract, Phaeophyceae extract, and mixed lavender oil to obtain phase C. Add phase B and phase C to phase A, and stir evenly. The stirring parameters are: 500 r / min, stirring for 25 min, to obtain the skin care composition.

[0134] In addition, the inventors also simultaneously provided Comparative Examples 1-12, and simultaneously tested the skin care compositions prepared in Example 1-4 and Comparative Examples 1-12 for moisturizing, soothing, and repairing functions, as follows.

[0135] Comparative Example 1-6: Preparation of skin care composition

[0136] Take Astragalus membranaceus root extract, glycerin, xanthan gum and disperse them in water, stir, heat to 83°C, mix evenly, and then cool to 40°C to obtain phase A. Take p-hydroxyacetophenone, methylpropanediol, stir, heat to 60°C, mix evenly, and then cool to 40°C to obtain phase B. Take Lactobacillus / soybean milk fermentation product filtrate, kava root extract, Phaeophyceae extract, and mixed lavender oil to obtain phase C. Add phase B and phase C to phase A, and stir evenly. The stirring parameters are: 500 r / min, stirring for 25 min, to obtain the skin care composition.

[0137] Test Example 1A: Evaluation of moisturizing efficacy

[0138] Evaluation method: Select volunteers aged 20-40 years old (number of people is 30, of which 15 are 20-30 years old and 15 are 30-40 years old), and the test product is a skin care composition with different formulations. Apply the test product to the inner side of the volunteer's left and right arms, and after 20 minutes, rinse with clean water. Use a skin tester (manufacturer: Antsci; model: Skin FX) to measure the moisture value of the skin after using the skin care composition for 20 minutes, 1 hour, and 2 hours, to obtain the moisture increase value after 20 minutes, 1 hour, and 2 hours. The moisture increase value is the average value of the moisture increase value after the test is used.

[0139] Wherein:

[0140] Moisture increase value = skin moisture value after using the product - skin moisture value before using the product

[0141] The moisture increase value results are shown in the following table:

[0142] From the above comparative results, it can be seen that the moisturizing effect of the skin care product composition of the present application with the addition of Astragalus membranaceus root extract, glycerol and methylpropanediol is obviously greater than that of the skin care product composition with the addition of only one or two of them.

[0143] Test Example 1B: soothing efficacy test

[0144] Test method one:

[0145] The soothing efficacy of the product is verified by evaluating the improvement of the irritation state of skin redness, itching, stinging, etc.

[0146] Lactic acid stinging experiment and subjective evaluation: 50ul of 10% lactic acid solution was dropped on the nasolabial sulcus, and the subjects were asked about their subjective symptoms at 2.5min and 5min, and scored from 0 to 3, 0 for no stinging, 1 for mild stinging, 2 for moderate stinging, and 3 for severe stinging. Then the scores of the two inquiry points were added together as the lactic acid stinging experiment results, and the total score ≥3 was positive.

[0147] Test method: select volunteers aged 20-40 years old (number of people is 30, of which 20-30 years old 15 people, 30-40 years old 15 people; lactic acid stinging experiment total score ≥3 people), test product is different formula skin care product composition. The test site is the nasolabial sulcus. Before the test, the subjects uniformly clean the face, and use a lint-free absorbent dry paper towel to absorb the moisture. Then the subjects are subjected to subjective evaluation of lactic acid stinging experiment, record the initial value, and use VISIA7 skin image analyzer to collect images after lactic acid stinging experiment. The subjective evaluation after applying the test product is recorded as the measurement value, and the VISIA 7 skin image analyzer is used to collect images after applying the test product. The measurement value is the mean value of the measurement value of the subjects.

[0148] Test method two:

[0149] The soothing efficacy of the product is verified by measuring the change in the proportion of the red area of the skin.

[0150] Test method: select the age between 20-40 years old volunteers (number of 30 people, of which 20-30 years old 15 people, 30-40 years old 15 people), the test product is different formula of skin care composition. The test site is the face. Before the test, the subjects are uniformly cleaned face, and the no dust water absorption dry paper towel is used to dry the moisture. Then the VISIA7 skin image analyzer is used to measure the skin red area ratio, and the initial value is recorded. After using the sample for 14 days, 28 days, the skin red area ratio of each test area is measured, and the measured value is recorded. The statistical method of skin red area ratio is the average value of skin red area ratio after the use of the subjects.

[0151] The test method is as follows:

[0152] The test method is as follows:

[0153] From the soothing effect test data obtained by test method one and two, it can be seen that the soothing effect of the skin care composition of example 1, comparative example 1, comparative example 4 and comparative example 5 is greater than that of comparative example 2, comparative example 3 and comparative example 6. It is proved that the soothing effect of the skin care composition added with the root extract of membranous huangqi is obviously greater than that without the root extract of membranous huangqi, which proves that the root extract of membranous huangqi has soothing effect in addition to moisturizing effect.

[0154] Test example 1C: repair effect test

[0155] By measuring the change of transdermal water loss rate and hemoglobin content of the skin, the change of skin barrier function is reflected, and the repair effect of the product is verified.

[0156] Test method: select the age between 20-40 years old volunteers (number of 30 people, of which 20-30 years old 15 people, 30-40 years old 15 people), the test product is different formula of skin care composition. The test site is the face. Before the test, the subjects are uniformly cleaned face, and the no dust water absorption dry paper towel is used to dry the moisture. Then the VISIA7 skin image analyzer is used to measure the skin red area ratio, and the initial value is recorded. After using the sample for 14 days, 28 days, the skin red area ratio of each test area is measured, and the measured value is recorded. The statistical method of skin red area ratio is the average value of skin red area ratio after the use of the subjects.

[0157] The repair effect test results are as follows:

[0158] Transdermal water loss value table

[0159] Hemoglobin content determination table

[0160] From the repair efficacy test data, it can be known that in the skin care product composition of the present application, the skin care product composition (Example 1, Comparative Examples 1-6) added with Astragalus membranaceus root extract has obvious decrease in skin water loss value and hemoglobin content after 14 days and 28 days, and the decrease in skin water loss value and hemoglobin content after 28 days is greater than that after 14 days; while the skin care product composition (Comparative Examples 2, 3 and 6) without Astragalus membranaceus root extract only has slight decrease in skin water loss value and hemoglobin content after 14 days and 28 days, and the decrease is less than that of the skin care product composition added with Astragalus membranaceus root extract, indicating that the repair effect of the skin care product composition added with Astragalus membranaceus root extract is obviously greater than that without Astragalus membranaceus root extract, and indicating that Astragalus membranaceus root extract has repair effect in addition to moisturizing effect.

[0161] Comparative Examples 6-9: Preparation of skin care product composition

[0162] Astragalus membranaceus root extract, glycerol and xanthan gum were dispersed in water, stirred, heated to 83℃, and uniformly mixed and cooled to 40℃ to obtain phase A. p-Hydroxyacetophenone and methylpropanediol were stirred, heated to 60℃, and uniformly mixed and cooled to 40℃ to obtain phase B. Lactobacillus / soybean milk fermentation filtrate, Kava root extract, Phaeophyceae extract and mixed lavender oil were uniformly mixed to obtain phase C. Phase B and phase C were added to phase A, and stirred uniformly at a stirring parameter of 500 r / min for 25 min to obtain the skin care product composition.

[0163] Test Example 2A: Moisturizing efficacy evaluation

[0164] Evaluation method: 30 volunteers aged 20-40 years old were selected (15 volunteers aged 20-30 years old and 15 volunteers aged 30-40 years old), and the test product was a skin care product composition with different formulations. The test product was applied to the inner side of the volunteer's left and right arms, and after 20 minutes, the skin was washed clean with clean water. A skin tester (manufacturer: Antsci; model: Skin FX) was used to measure the moisture value of the skin after using the skin care product composition for 20 minutes, 1 hour and 2 hours, and the moisture increase value after 20 minutes, 1 hour and 2 hours was obtained. The moisture increase value was calculated as the average value of the moisture increase value after using the test product.

[0165] Wherein:

[0166] Moisture increase value = skin moisture measurement value after using product - skin moisture measurement value before using product

[0167] The moisture increase value results are shown in the following table:

[0168] From the above comparison results, it can be seen that the moisture increase value of the skin care composition of Example 1, Example 2, Comparative Example 7, Comparative Example 8, Comparative Example 9 in the skin care composition of the application is greater than that of Comparative Example 6, and the moisture increase value of Example 1, Example 2 is greater than that of Comparative Example 7, Comparative Example 8, Comparative Example 9. It is shown that the moisturizing effect of the skin care composition added with the root extract of Astragalus membranaceus is greater than that of the skin care composition without the root extract of Astragalus membranaceus, and the root extract of Astragalus membranaceus in the range of 1%-2% has more obvious moisturizing effect.

[0169] Test Example 2B: soothing efficacy test

[0170] Test method one:

[0171] By evaluating the improvement of skin redness, itching, stinging and other irritation, the soothing effect of the product is verified.

[0172] Lactic acid stinging test and subjective evaluation: take 50ul of 10% lactic acid solution, drop it on the nasolabial sulcus, ask the subjects for subjective symptoms at 2.5min and 5min, score 0-3, 0 for no stinging, 1 for mild stinging, 2 for moderate stinging, 3 for severe stinging, then add the scores of the two inquiry points, as the lactic acid stinging test result, total score≥3 is positive.

[0173] Test method: select volunteers aged 20-40 years old (number of people is 30, of which 20-30 years old 15 people, 30-40 years old 15 people; lactic acid stinging test total score≥3 people), test product is different formula skin care composition. The test site is the nasolabial sulcus. Before the test, the subjects uniformly clean the face, and use a lint-free absorbent dry paper towel to absorb the moisture. Then the subjects are subjected to subjective evaluation of lactic acid stinging test, the initial value is recorded, and VISIA7 skin image analyzer is used for image acquisition after lactic acid stinging test. The subjective evaluation after applying the test product is recorded as the measurement value, and VISIA 7 skin image analyzer is used for image acquisition after applying the test product. The measurement value is the mean value of the measurement value of the subjects.

[0174] Test method two:

[0175] By measuring the change of the area ratio of the red area of the skin, the soothing effect of the product is verified.

[0176] Test method: Select volunteers aged 20-40 years old (30 people, 15 of which are 20-30 years old and 15 of which are 30-40 years old), and the test product is a skin care composition with different formulations. The test site is the face. Before the test, the subjects uniformly clean the face and use a lint-free absorbent dry paper towel to absorb moisture. Then use the VISIA7 skin image analyzer to measure the area ratio of the red area of the skin, and record the initial value. After 14 days and 28 days of use of the sample, measure the area ratio of the red area of the skin in each test area, and record the measured value. The statistical method of the area ratio of the red area of the skin is the average value of the area ratio of the red area of the skin after the subjects use it.

[0177] The results of the soothing efficacy test according to Test Method I are as follows:

[0178] The results of the soothing efficacy test according to Test Method II are as follows:

[0179] From the soothing efficacy test data obtained by Test Methods I and II, it can be seen that the soothing effect of the skin care composition of Example 1, Example 2, Comparative Example 7, Comparative Example 8, and Comparative Example 9 is greater than that of Comparative Example 6, and the soothing effect of Example 1 and Example 2 is greater than that of Comparative Example 7, Comparative Example 8, and Comparative Example 9. It is shown that the soothing effect of the skin care composition added with the film-walled Astragalus root extract is greater than that of the skin care composition without the film-walled Astragalus root extract, and the film-walled Astragalus root extract is in the range of 1%-2%, and the soothing effect is more obvious.

[0180] Test Example 2C: Repairing Efficacy Test

[0181] By measuring the changes in transdermal water loss rate and hemoglobin content of the skin, the changes in skin barrier function are reflected, and the repairing efficacy of the product is verified.

[0182] Test method: select volunteers aged 20-40 years old (number of people is 30, of which 20-30 years old 15 people, 30-40 years old 15 people), the test product is different formula skin care composition. The test site is the inner side of the arm. Use low sensitization tape to paste the test area, press for 2 seconds, then slowly peel off, continuously tear off until visible erythema appears, respectively measure the transdermal water loss value and hemoglobin content of each test area with skin moisture tester (Corneometer@CM825), skin melanin and hemoglobin tester (Mexameter@MX18), record as initial value. After using sample for 14 days, 28 days, measure the transdermal water loss value and hemoglobin content of each test area, record as measurement value. The statistical method of transdermal water loss value is the average value of transdermal water loss value after use by the subject; the statistical method of hemoglobin content is the average value of hemoglobin content after use by the subject.

[0183] The repair effect test results are as follows:

[0184] Transdermal water loss value table

[0185] Hemoglobin content determination table

[0186] From the repair effect test data, it can be seen that in the skin care composition of the application, the skin moisture loss value and hemoglobin content of the skin care composition of example 1, example 2, comparative example 7, comparative example 8, comparative example 9 after 14 days, 28 days all appear obvious decrease, and the skin moisture loss value and hemoglobin content after 28 days decrease value is greater than the skin moisture loss value and hemoglobin content after 14 days; while the skin moisture loss value and hemoglobin content of the skin care composition of comparative example 6 after 14 days, 28 days only appear slightly decrease, and the decrease value is less than the decrease value of example 1, example 2, comparative example 7, comparative example 8, comparative example 9; and the decrease value of the skin moisture loss value and hemoglobin content of the skin care composition of example 1, example 2 after 14 days, 28 days is obviously greater than the decrease value of the skin moisture loss value and hemoglobin content of the skin care composition of comparative example 7, comparative example 8, comparative example 9 after 14 days, 28 days. It shows that the repair effect of the skin care composition added with Astragalus membranaceus root extract is greater than that of the skin care composition without Astragalus membranaceus root extract, and the repair effect is more obvious when the Astragalus membranaceus root extract is in the range of 1%-2%.

[0187] Comparative examples 10-12: preparation of skin care composition

[0188] Take the Astragalus membranaceus root extract, glycerin, xanthan gum, disperse in water, stir, heat to 83℃, mix evenly, cool to 40℃, get phase A. Take p-hydroxyacetophenone, methylpropylene glycol, stir, heat to 60℃, mix evenly, cool to 40℃, get phase B. Take Lactobacillus / soybean milk fermentation product filtrate, kava root extract, Ascophyllum nodosum extract, mixed lavender oil, mix evenly, get phase C. Add phase B, phase C to phase A, stir evenly, the stirring parameters are: 500r / min, stir for 25min, get the skin care product composition.

[0189] Test example 3A: evaluate the moisturizing effect

[0190] Evaluation method: select volunteers aged 20-40 years old (the number of people is 30, of which 15 are 20-30 years old and 15 are 30-40 years old), the test product is a skin care product composition with different formulations. The test product is applied to the inner side of the volunteer's left and right arm, and then washed off with clean water after 20 minutes. The skin moisture value after using the skin care product composition for 20 minutes, 1 hour and 2 hours is measured by a skin tester (manufacturer: Antsci; model: Skin FX), and the moisture increase value after 20 minutes, 1 hour and 2 hours is obtained. The moisture increase value is the average value of the moisture increase value after the test is used.

[0191] Among them:

[0192] Moisture increase value = skin moisture value after using the product - skin moisture value before using the product

[0193] The moisture increase value results are shown in the following table:

[0194] From the above comparison results, it can be seen that the moisture increase value of the skin care product composition of example 1, example 3, example 4, comparative example 11 and comparative example 12 is greater than that of comparative example 10, and the moisture increase value of example 1, example 3 and example 4 is greater than that of comparative example 11 and comparative example 12. It is shown that the moisturizing effect of the skin care product composition added with mixed lavender oil is greater than that of the skin care product composition without mixed lavender oil, and the moisturizing effect is more obvious when the mixed lavender oil is in the range of 0.1%-0.3%. Therefore, it can be concluded that the mixed lavender oil in the skin care product composition of the present application has a moisturizing effect completely different from its conventional effect (relieving, etc.).

[0195] Test example 3B: soothing effect test

[0196] Test method one:

[0197] The soothing effect of the product is verified by evaluating the improvement of skin redness, itching, stinging and other irritation.

[0198] Lactic acid stinging test and subjective evaluation: 50ul of 10% lactic acid solution was dropped on nasolabial sulcus, and the subjects were asked about their subjective symptoms at 2.5min and 5min, and the scores were graded from 0 to 3, 0 for no stinging, 1 for mild stinging, 2 for moderate stinging, and 3 for severe stinging. Then the scores of the two inquiry points were added together as the lactic acid stinging test results, and the total score ≥3 was positive.

[0199] Test method: Select volunteers aged 20-40 years old (number of people is 30, including 15 people aged 20-30 years old and 15 people aged 30-40 years old; the total score of lactic acid stinging test is ≥3), and the test product is a skin care composition with different formulations. The test site is the nasolabial sulcus. Before the test, the subjects uniformly clean their faces and use a lint-free absorbent dry paper towel to absorb the moisture. Then the subjects are subjected to subjective evaluation of the lactic acid stinging test, and the initial value is recorded. The VISIA7 skin image analyzer is used for image acquisition after the lactic acid stinging test. The subjective evaluation after applying the test product is recorded as the measurement value, and the VISIA7 skin image analyzer is used for image acquisition after applying the test product. The measurement value is the mean value of the measurement value of the subjects.

[0200] Test method two:

[0201] The soothing efficacy of the product is verified by measuring the change in the proportion of skin red area.

[0202] Test method: Select volunteers aged 20-40 years old (number of people is 30, including 15 people aged 20-30 years old and 15 people aged 30-40 years old), and the test product is a skin care composition with different formulations. The test site is the face. Before the test, the subjects uniformly clean their faces and use a lint-free absorbent dry paper towel to absorb the moisture. Then the VISIA7 skin image analyzer is used to measure the proportion of skin red area, and the initial value is recorded. After using sample 14 days and 28 days, the proportion of skin red area in each test area is measured, and the measurement value is recorded. The proportion of skin red area is the mean value of the proportion of skin red area after the subjects use it.

[0203] The soothing efficacy test results of test method one are as follows:

[0204] The soothing efficacy test results of test method two are as follows:

[0205] The soothing effect test data obtained by the test methods one and two show that the soothing effects of the skin care product compositions of Example 1, Example 3, Example 4, Comparative Example 11 and Comparative Example 12 are all greater than that of Comparative Example 10, and the soothing effects of Example 1, Example 3 and Example 4 are greater than those of Comparative Example 11 and Comparative Example 12. It is illustrated that the soothing effects of the skin care product compositions added with the mixed lavender oil are all greater than those of the skin care product compositions without the mixed lavender oil, and the soothing effects are more obvious when the mixed lavender oil is in the range of 0.1% to 0.3%.

[0206] Test Example 3C: Repairing effect test

[0207] The repairing effect of the product is verified by measuring the changes of the transdermal water loss rate and the hemoglobin content of the skin, reflecting the changes of the skin barrier function.

[0208] Test method: select volunteers aged 20-40 years old (the number of people is 30, of which 15 are 20-30 years old and 15 are 30-40 years old), and the test product is a skin care product composition with different formulations. The test site is the inner side of the arm. Use a low-sensitization adhesive tape to paste the test area, press for 2 seconds and then slowly peel off, continuously tear off until visible erythema appears. The transdermal water loss value and hemoglobin content of each test area are measured by a skin moisture tester (Corneometer@CM825), a skin melanin and hemoglobin tester (Mexameter@MX18), respectively, and recorded as the initial value. After using the sample for 14 days and 28 days, the transdermal water loss value and hemoglobin content of each test area are measured, and recorded as the measured value. The transdermal water loss value statistical method is the mean value of the transdermal water loss value after use by the subjects; the hemoglobin content statistical method is the mean value of the hemoglobin content after use by the subjects.

[0209] The repairing effect test results are as follows:

[0210] Transdermal water loss value table

[0211] Hemoglobin content determination table

[0212] It can be known from the repair effect test data that the skin care product composition of Example 1, Example 3, Example 4, Comparative Example 11 and Comparative Example 12 has obvious decrease in skin moisture loss value and hematin content after 14 days and 28 days, and the decrease in skin moisture loss value and hematin content after 28 days is greater than the decrease in skin moisture loss value and hematin content after 14 days; the skin care product composition of Comparative Example 10 has decrease in skin moisture loss value and hematin content after 14 days and 28 days, but the decrease is less than the decrease of Example 1, Example 3, Example 4, Comparative Example 11 and Comparative Example 12, and the decrease in skin moisture loss value and hematin content of the skin care product composition of Example 1, Example 3 and Example 4 after 14 days and 28 days is obviously greater than the decrease in skin moisture loss value and hematin content of the skin care product composition of Comparative Example 11 and Comparative Example 12 after 14 days and 28 days. It is illustrated that the repair effect of the skin care product composition added with the mixed lavender oil is greater than the repair effect of the skin care product composition without the mixed lavender oil, and the mixed lavender oil in the range of 0.1% to 0.3% has obvious improvement in repair effect, which illustrates that the mixed lavender oil has the repair effect in addition to the soothing effect.

[0213] Finally, it should be noted that for those skilled in the art to which the present application belongs, the architecture form can be flexible and variable without departing from the concept of the present application, and simple deduction or replacement should be regarded as belonging to the patent protection scope determined by the claims of the present application.

Claims

1. A cosmetic composition comprising: Membrane Astragalus membranaceus root extract, glycerin, methylpropanediol, soothing repair ingredients, antioxidants, thickening agents, and water, wherein, 1-2% of Membrane Pod Root Extract of Astragalus membranaceus (Fisch.) Bunge, 2. The cosmetic composition of claim 1, comprising: 1-2% of Membrane Pod Root Extract of Astragalus membranaceus (Fisch.) Bunge, 0.1-10% of glycerin, 1-10% of methylpropanediol, 1-20% of soothing repair ingredient, 0.01-1% of antioxidant, 0.01-1% of thickening agent, and balance of water.

3. The cosmetic composition according to any one of claims 1-2, wherein, The soothing repair ingredient is selected from microbial fermentation product, plant extract, algal extract, plant oil, or any combination thereof; Preferably, the cosmetic composition comprises 0.1-0.3% of the plant oil by weight.

4. The cosmetic composition according to claim 3, wherein, The cosmetic composition further has one or more technical features selected from (i)-(iv) below: (i) the microbial fermentation product is Lactobacillus / soybean milk fermentation product filtrate; (ii) the plant extract is Kava Root Extract; (iii) the algal extract is Phaeodactylum tricornutum extract; (iv) the plant oil is Lavandula hybrida oil.

5. The cosmetic composition of claim 4, comprising: 1-2% of Membrane Pod Root Extract of Astragalus membranaceus (Fisch.) Bunge, 0.1-10% of glycerin, 1-10% of methylpropanediol, 1-5% of Lactobacillus / soybean milk fermentation product filtrate, 1-5% of Kava Root Extract, 1-5% of Phaeodactylum tricornutum extract, 0.1-0.3% of Lavandula hybrida oil, 0.01-1% of antioxidant, 0.01-1% of thickening agent, and balance of water.

6. The cosmetic composition of any one of claims 4-5, comprising: 1-2% of Membrane Pod Root Extract of Astragalus membranaceus (Fisch.) Bunge, 1-3% of glycerin, 5-7% of methylpropanediol, 1-3% of Lactobacillus / soybean milk fermentation product filtrate, 1-2% of Kava Root Extract, 1-3% of Phaeodactylum tricornutum extract, 0.1-0.3% of Lavandula hybrida oil, 0.4-0.6% of antioxidant, 0.1-0.3% of thickening agent, and balance of water.

7. The cosmetic composition of any one of claims 4-6, comprising: 2% of Membrane Pod Root Extract of Astragalus membranaceus (Fisch.) Bunge, 2% of glycerin, 6% of methylpropanediol, 2% of Lactobacillus / soybean milk fermentation product filtrate, 1.5% of Kava Root Extract, 2% of Phaeodactylum tricornutum extract, 0.1% of Lavandula hybrida oil, 0.5% of antioxidant, 0.2% of thickening agent, and balance of water; or, comprising: 1% of Membrane Pod Root Extract of Astragalus membranaceus (Fisch.) Bunge, 2% of glycerin, 6% of methylpropanediol, 2% of Lactobacillus / soybean milk fermentation product filtrate, 1.5% of Kava Root Extract, 2% of Phaeodactylum tricornutum extract, 0.1% of Lavandula hybrida oil, 0.5% of antioxidant, 0.2% of thickening agent, and balance of water; or, comprising: 2% of Membrane Pod Root Extract of Astragalus membranaceus (Fisch.) Bunge, 2% of glycerin, 6% of methylpropanediol, 2% of Lactobacillus / soybean milk fermentation product filtrate, 1.5% of Kava Root Extract, 2% of Phaeodactylum tricornutum extract, 0.2% of Lavandula hybrida oil, 0.5% of an antioxidant, 0.2% of a thickening agent, and the balance water; or, it comprises: 2% of a membrane podocarpus root extract, 2% of glycerin, 6% of methyl propanediol, 2% of a lactobacillus / soybean milk fermentation product filtrate, 1.5% of a kava root extract, 2% of a trepoclonium fuciforme extract, 0.3% of a mixed lavender oil, 0.5% of an antioxidant, 0.2% of a thickening agent, and the balance water.

8. The cosmetic composition according to any one of claims 1-7, wherein, The cosmetic composition has one or more technical features selected from the following (i)-(ii): (i) the antioxidant is selected from butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), tertiary butylhydroquinone (TBHQ), propyl gallate, rubusoside, p-hydroxyacetophenone or any combination thereof; Preferably, the antioxidant is p-hydroxyacetophenone; (ii) the thickening agent is selected from xanthan gum, magnesium aluminum silicate, agar, carbomer, sodium alginate or any combination thereof; Preferably, the thickening agent is xanthan gum.

9. A cosmetic comprising the cosmetic composition of any one of claims 1-8.

10. A method for preparing the cosmetic composition of any one of claims 1-8, comprising: dispersing the membrane podocarpus root extract, glycerin and thickening agent in water, heating to 80-85°C (e.g. 83°C), carrying out a first mixing, cooling to 30-50°C (e.g. 40°C) after mixing is uniform, obtaining phase A; mixing the antioxidant and methyl propanediol, heating to 60-65°C (e.g. 60°C), cooling to 30-50°C (e.g. 40°C) after mixing is uniform, obtaining phase B; carrying out a third mixing of the soothing repair ingredients, obtaining phase C after mixing is uniform; adding the phase B and the phase C to the phase A, carrying out a fourth mixing, obtaining the cosmetic composition after mixing is uniform; Preferably, the fourth mixing is carried out at a stirring speed of 400-600 r / min (e.g. 500 r / min); Preferably, the fourth mixing is carried out for 20-30 min (e.g. 25 min).

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