Fat cutting filter, fat cutting and filtering method, and fat cutting and filtering device

Abstract

A fat cutting filter, a fat cutting and filtering method, and a fat cutting and filtering device. The fat cutting filter comprises: a housing (100), the interior of the housing (100) being a treatment cavity (130), and the housing (100) being provided with a first interface (111) and a second interface (121) both communicated with the treatment cavity (130); and a cutting member (200) located in the treatment cavity (130) and provided between the first interface (111) and the second interface (121), the cutting member (200) comprising a rigid wire mesh (210) for fat cutting, and the wire diameter of the rigid wire mesh (210) ranging from 0.05 mm to 1 mm.

Description

Fat-cutting filter, fat-cutting filtering method and fat-cutting filtering device

[0001] Related applications

[0002] The present application claims priority to the Chinese patent application No. 202411254785.5, filed on September 6, 2024, entitled “Fat-cutting filter, fat-cutting filtering method and fat-cutting filtering device”, the content of which is incorporated herein by reference in its entirety. TECHNICAL FIELD

[0003] The present application relates to the technical field of medical devices, in particular to a fat-cutting filter, a fat-cutting filtering method and a fat-cutting filtering device. BACKGROUND

[0004] Vascular stromal fraction (SVF) is derived from mature adipose tissue, and is a group of heterogeneous cell populations containing adipose-derived stem cells (ADSCs), endothelial progenitor cells (EPCs), hematopoietic stem cells (HPCs), etc. Studies have reported that ADSCs obtained by adherent culture of SVF in vitro can differentiate into osteoblasts, chondrocytes, adipocytes, etc. under different induction environments; and literature shows that SVF can secrete a large amount of pro-angiogenic factors, and at the same time can differentiate into endothelial cells to participate in vascular sprouting.

[0005] Autologous fat transplantation refers to the transplantation of autologous adipose tissue obtained from a donor area by surgical incision or negative pressure aspiration, etc. after processing in vitro, including rinsing, cutting, centrifugation, purification, adding autologous stem cells or allogeneic cytokines, drugs, etc. to achieve the purpose of tissue transplantation technology to increase tissue volume, promote tissue regeneration or improve tissue texture, etc.

[0006] Adipose tissue has been considered an endocrine organ due to its role in energy regulation, inflammatory response, and immune response. In addition, it also has multi-potential and multi-functional cells, and such multi-functional cells exist in the vascular stromal fraction (SVF) of adipose tissue.

[0007] The vascular stromal fraction (SVF) in adipose tissue is a group of multi-phase cell populations obtained after filtering and centrifuging mature adipocytes from adipose tissue. Because SVF is easy to extract from adipose tissue and contains abundant, highly plastic, and multi-directional differentiation adipose tissue-derived mesenchymal stem cells (ADSCs), SVF can be directly used to treat inflammation and pain of osteoarthritis, and can also be used to improve facial fat atrophy, etc. At present, many clinical trials are actively recruiting volunteers to evaluate the effect of SVF treatment on related diseases, so it is of great significance to reveal the cell composition of SVF and elucidate its corresponding biological functions.

[0008] SVF cells represent a heterogeneous population of cells that surround adipocytes in adipose tissue. SVF obtained from human breast and visceral adipose tissue contains endothelial cells, non-characteristic stromal cells, blood cells, and tissue macrophages. In addition, SVF is also a source of hematopoietic progenitor cells and endothelial stem cells.

[0009] SVF is usually prepared by enzymatic decomposition, mechanical separation, etc. The traditional enzyme digestion method is mainly completed by manual operation, which is carried out in an open environment through a centrifuge tube and a pipetting device. The operation steps are complex, the quality of the operator is required to be high, the cell loss is large during the operation process, and there is a risk of pollution. The mechanical method is favored by the clinic because of its simple operation and rapid preparation. The current market mainly uses mechanical separation method to extract adipose tissue from patients or animals, and then uses cutting and filtering combined with centrifugation to obtain adipose cells and separate SVF from adipose tissue.

[0010] For example, the adipose tissue is obtained by suction with a liposuction needle or a liposuction device, and then transferred to a cutting and crushing device for crushing. After crushing, it is transferred to a centrifuge tube for centrifugal separation, so as to obtain the effective material that needs to be collected, mainly the cell cluster-SVF (adipose-derived stem cells, endothelial progenitor cells, etc.) and extracellular matrix, which play an important role in tissue regeneration.

[0011] When the manual cutting and filtering method is used, a cutting filter containing one or more cutting filters is used. Two syringes are connected at both ends of the filter for repeated pushing and pulling. During the process of passing through the pores of the cutting filter, the mature adipocytes with large volume are broken due to the extrusion and shearing effect, so as to complete the cutting and filtering, and the SVF cells with small volume are not damaged.

[0012] This mechanical cutting and centrifugation removes most of the lipids and other useless components (such as swelling liquid) in the fat. This preparation method is simple and easy to operate, and does not need to use any chemical drugs or animal-derived preparations, so as to obtain SVF by mechanical force treatment.

[0013] However, in actual application, the cutting process is time-consuming and laborious, the cutting efficiency is low, the effect is poor, and even the treatment fails due to the problems of too much fascia in fat, large fat particles, or the problems of small filter hole diameter, irregular hole type and low porosity, etc. The shape of the pore is not ideal, which affects the use, and the unqualified product rate is high, and the processing cost is high. Although the large-diameter filter screen saves labor, the quality of fat cutting and crushing is sacrificed.

[0014] On the other hand, the fat particle size of the target is difficult to cut at one time due to the cutting resistance, so it needs to be cut through different aperture filter screens one or more times, the operation is complex and difficult, and because the cavity volume of the cutter is large, more fat will be left in the cutter, and the fat yield after cutting is low.

[0015] The cutting screen on the market at present mainly adopts irregular mesh design, the purpose of which is to improve the cutting performance, but the irregular shape design of the cutting screen will lead to greater processing difficulty (chemical etching, laser processing, etc.), higher processing quality control difficulty (the shape of the processed holes is unstable), unstable filter screen quality, affecting the cutting efficiency, and the cutting device is complex to process and has high processing cost. SUMMARY

[0016] The application provides a fat cutting filter with simple structure and high fat cutting utilization rate.

[0017] The fat cutting filter provided by the application comprises a shell, the inside of the shell comprises a treatment cavity, the shell is provided with a first interface and a second interface which are in communication with the treatment cavity; a cutting member is located in the treatment cavity and between the first interface and the second interface, the cutting member comprises a rigid wire screen for cutting fat, and the wire diameter of the rigid wire screen is 0.05mm-1mm.

[0018] The following also provides several optional modes, but not as an additional limitation to the above general scheme, just a further supplement or preferred, without technical or logical contradiction, each optional mode can be combined with the above general scheme alone, and can also be combined between multiple optional modes.

[0019] In one of the embodiments, the cutting member further comprises a support member, and the rigid wire screen is tightly fixed to the support member.

[0020] In one of the embodiments, the porosity of the rigid wire screen is greater than or equal to 80%. The porosity=(s1 / s)×100%, wherein s1 is the open area of the rigid wire screen, and s is the total area of the rigid wire screen.

[0021] In one of the embodiments, the porosity of the rigid wire screen is greater than or equal to 90%.

[0022] In one of the embodiments, the wire diameter of the rigid wire screen is 0.05mm-0.5mm.

[0023] In one of the embodiments, the ratio of the pore diameter to the wire diameter of the rigid wire screen is 8:1-1:1.

[0024] In one embodiment, the wire diameter of the rigid wire mesh is 0.15mm-0.50mm, and the ratio of the hole diameter to the wire diameter of the rigid wire mesh is 5:1-1:1.

[0025] In one embodiment, the rigid wire mesh is a woven mesh, and the weaving manner of the woven mesh is plain weaving or twist weaving.

[0026] In one embodiment, the hole diameter of the rigid wire mesh is 300μm-3000μm.

[0027] In one embodiment, the hole diameter of the rigid wire mesh is 300μm-2500μm.

[0028] In one embodiment, the wire diameter of the rigid wire mesh is 0.15mm-0.30mm, and the hole diameter is 500μm-1500μm.

[0029] In one embodiment, the mesh hole shape of the rigid wire mesh is polygonal.

[0030] In one embodiment, the polygon is triangular, quadrangular or hexagonal.

[0031] In one embodiment, the rigid wire mesh is woven by rigid wires, and the cross-sectional shape of the rigid wire is circular, elliptical or rectangular.

[0032] In one embodiment, the cutting member comprises a layer of rigid wire mesh or multiple layers of rigid wire mesh arranged at intervals, and the hole diameters of the multiple layers of rigid wire mesh are arranged in sequence.

[0033] In one embodiment, the cutting member comprises multiple layers of rigid wire mesh arranged at intervals along the first direction, and the adjacent two layers of rigid wire mesh are arranged in at least one of the following manners: a) the mesh hole shapes of each layer of rigid wire mesh are different; b) the hole diameters of each layer of rigid wire mesh are different; c) the wire diameters of each layer of rigid wire mesh are different; d) along the first direction, the rigid wires of each layer of rigid wire mesh extend in different directions.

[0034] In one embodiment, the hole diameter of one of the adjacent two layers of rigid wire mesh is defined as D1, the hole diameter of the other is defined as D2, and the distance between the adjacent two layers of mesh is defined as L, and D1, D2 and L satisfy the following relationship: 0.5(D1+D2)≤L≤3(D1+D2).

[0035] In one embodiment, D1, D2 and L satisfy the following relationship: 0.6(D1+D2)≤L≤2.5(D1+D2).

[0036] In one embodiment, D1, D2 and L satisfy the following relationship: 0.8(D1+D2)≤L≤1.5(D1+D2).

[0037] The application also provides a fat cutting filter, comprising: a shell, an inner part of the shell comprising a processing cavity, the shell being provided with a first interface and a second interface communicating with the processing cavity; a cutting member located in the processing cavity and between the first interface and the second interface, at least a part of the cutting member being a working area for cutting and filtering fat, the working area having a porosity of 80% or more.

[0038] In one embodiment, the cutting member comprises: a support ring, which is configured separately or is a part of the shell; a rigid wire mesh fixed in the support ring and providing the working area, the wire diameter of the rigid wire mesh being 0.15mm-0.30mm, and the pore diameter being 500μm-1500μm.

[0039] In one embodiment, the working area is one.

[0040] In one embodiment, the working area is a plurality of working areas arranged at intervals, and the adjacent working areas are reinforced parts fixed in the support ring.

[0041] The application also provides a processing method of a fat cutting filter, comprising:

[0042] providing a rigid wire, pre-encoding the rigid wire to obtain a rigid wire mesh;

[0043] providing a support ring, cutting the rigid wire mesh according to a predetermined shape, and tightly fixing the cut rigid wire mesh to the support ring;

[0044] providing a shell, the shell being provided with a first interface and a second interface, and the cutting member being encapsulated in the shell and located between the first interface and the second interface.

[0045] The application also provides a fat cutting and filtering method, comprising: driving fat tissue to be processed through a predetermined channel; pre-arranging a rigid wire mesh in the predetermined channel, and cutting the fat tissue through the wire of the rigid wire mesh, the wire diameter of the rigid wire mesh being 0.05mm-1mm; along the flow direction of the fat tissue, the rigid wire mesh is one layer or a plurality of layers arranged at intervals, and the adjacent two layers of the plurality of layers of the rigid wire mesh are arranged in at least one of the following ways: a) the mesh hole shape of each rigid wire mesh is different; b) the pore diameter of each rigid wire mesh is different; c) the wire diameter of each rigid wire mesh is different; d) along a first direction, the rigid wire of each rigid wire mesh extends in different directions.

[0046] In one embodiment, the fat tissue passes through the rigid wire mesh along a single flow direction or reciprocally in two directions.

[0047] The application further provides a fat cutting filter device, comprising: a fat cutting filter according to the application; and a driving device in communication with the first interface and the second interface, which drives the fat tissue to be processed to pass through the cutting member.

[0048] Compared with the related art, the application has the following beneficial effects.

[0049] 1) The fat cutting filter according to the application has a cutting filter screen made of a rigid wire screen woven by superfine wire, the pore size of the cutting filter screen can be customized as required, the porosity of the cutting filter screen can reach more than 90%, the mesh of the cutting filter screen is more regular, the pore size is more uniform, and the cutting efficiency is higher, so that the problem of fat cutting filter blockage can be effectively solved; meanwhile, the weaving process is simpler than the traditional chemical etching and laser processing process, and has a processing cost advantage. In addition, the fat cutting filter with multiple layers of rigid wire screens can make fat cutting more simple and efficient, and improve the utilization rate and total amount of the cutting product.

[0050] 2) The SVF product after cutting by the fat cutting filter according to the application has more excellent physical properties and biological indexes: lower free oil and free water content; higher total amount of fat tissue cells and cell survival rate, higher content of adipose-derived stem cells, and better potential for promoting tissue repair and regeneration.

[0051] The details of one or more embodiments of the application are presented in the following drawings and description. Other features, objects, and advantages of the application will become apparent from the description, drawings, and claims. BRIEF DESCRIPTION OF DRAWINGS

[0052] In order to more clearly illustrate the technical solutions in the embodiments of the application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description only some embodiments of the application, and for those skilled in the art, other drawings can also be obtained without creative labor on the basis of these drawings.

[0053] Fig. 1 is a structural schematic diagram of a fat cutting filter according to an embodiment of the application.

[0054] Fig. 2 is a structural schematic diagram of the fat cutting filter in Fig. 1 from another angle.

[0055] Fig. 3 is a structural schematic diagram of a fat cutting filter (single-layer cutting member) according to an embodiment of the application.

[0056] Fig. 4 is an enlarged view of part A in Fig. 3.

[0057] Fig. 5 is a structural schematic diagram of a fat cutting filter (multi-layer cutting member) according to another embodiment of the application.

[0058] Fig. 6 is an exploded view of the multi-layer cutting member of Fig. 5.

[0059] Fig. 7 is a sectional view of the fat cutting filter of Fig. 5.

[0060] Fig. 8 is a schematic view of a multi-layer rigid wire screen arrangement.

[0061] Fig. 9 is a B-B sectional view of Fig. 8.

[0062] Fig. 10 is a schematic view of a fat cutting filter device according to an embodiment of the present application.

[0063] Fig. 11 is a schematic view of a fat cutting filter device according to another embodiment of the present application.

[0064] Fig. 12 is a comparison chart of fat content after cutting according to Test Example 1 of the present application.

[0065] Fig. 13 is a comparison chart of free oil and free water after cutting according to Test Example 2 of the present application.

[0066] Fig. 14 is a comparison chart of SVF volume after cutting according to Test Example 3 of the present application.

[0067] The reference signs of the elements are as follows: 10, fat cutting filter; 100, housing; 110, first half housing; 111, first interface; 120, second half housing; 121, second interface; 122, positioning member; 130, processing cavity; 200, cutting member; 200a, first cutting member; 200b, second cutting member; 200c, third cutting member; 210, rigid wire screen; 211, rigid wire; 212, mesh; 210a, warp; 210b, weft; 220, support ring; 230, working area; 300, retainer; 400, driving device; 410, pipeline; 420, first pump body; 430, second pump body. DETAILED DESCRIPTION

[0068] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0069] It is to be noted that when a component is referred to as being "on" or "disposed on" another component, it can be directly on the other component or intervening components can also be present. When a component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components can also be present. The terms "vertical", "horizontal", "upper", "lower", "left", "right", and similar terms as used in the description of the present application are for the purpose of illustration only and do not indicate the only position of the embodiment.

[0070] In addition, the terms "first", "second", and the like, are used merely as labels for convenience and do not imply or constitute a description of relative importance or a limitation on the number of features to which the label is applied. Thus, a feature defined with "first", "second" can include at least one of the features explicitly or implicitly. In the description of the present application, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise explicitly specified and limited.

[0071] In the present application, unless otherwise explicitly specified and limited, the "on", "under", "above" and "over" of the first feature to the second feature can be that the first feature is in direct contact with the second feature, or the first feature is indirectly in contact with the second feature through an intermediate medium. Moreover, the "on", "above" and "over" of the first feature to the second feature can be that the first feature is directly above or obliquely above the second feature, or only indicates that the first feature is higher than the second feature in horizontal height (or in a use state, or in a certain drawing perspective). The "under", "below" and "under" of the first feature to the second feature can be that the first feature is directly below or obliquely below the second feature, or only indicates that the first feature is lower than the second feature in horizontal height (or in a use state, or in a certain drawing perspective).

[0072] Unless otherwise defined, all technical and scientific terms used in the specification of the present application have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs. The terms used in the specification of the present application are only for the purpose of describing the specific embodiments and are not intended to limit the present application. The term "and / or" used in the specification of the present application includes any and all combinations of one or more of the associated listed items.

[0073] In the present application, the first direction is the direction from the first interface 111 to the second interface 121.

[0074] Referring to FIGS. 1-7, an embodiment of the present application provides a fat cutting filter, which includes a shell 100 and a cutting member 200. In order to facilitate processing and assembly, the shell 100 can adopt a split buckle fixing manner, for example, the shell 100 includes a first half shell 110 and a second half shell 120, which can be fixed by ultrasonic welding to enclose a processing cavity inside the shell 100 for cutting and filtering fat tissue.

[0075] In order to communicate with external pipelines or devices, the shell 100 is provided with a first interface 111 and a second interface 121 which communicate with the processing cavity, and each interface is arranged on the corresponding half shell.

[0076] The shell 100 has a middle part and two opposite ends, the middle part extends at the same diameter, and the shape of the two ends gradually converges until forming a tubular interface, i.e. the first interface 111 and the second interface 121 respectively, and the specific structure can adopt a luer joint or the like to facilitate quick assembly.

[0077] The cutting member 200 is located in the processing cavity, and is located in the middle part of the shell 100. The cutting member 200 is located between the first interface 111 and the second interface 121, that is, the fat tissue entering and exiting through each interface flows through the cutting member 200 to be cut and filtered. As one of the improvements of the present application, the cutting member 200 adopts a rigid wire mesh, wherein the "rigidity" is not strictly limited to the elastic modulus of the material. As a mesh structure, at least the mesh surface is not prone to concave and convex deformation during use, and the mesh hole shape and size remain stable to ensure the cutting and filtering effect. Each embodiment below takes the rigid wire mesh 210 as an example. In order to ensure the "rigidity" and the cutting effect, the rigid wire can be made of stainless steel or the like, and after being tightened, the mesh structure as a whole has high strength and is not prone to deformation under normal use conditions. The rigid wire mesh 210 is composed of rigid wires 211. From the perspective of the local action position, the rigid wire 211 applies linear cutting to the fat tissue, that is, using a wire as a blade. As a whole, the cutting member 200 can obtain greater porosity, and the fat cutting and filtering are not prone to blockage, and the operation is labor-saving.

[0078] In one embodiment, in order to maintain the shape and spatial posture of the rigid wire 211, the cutting member 200 further includes a support member, and the rigid wire 211 is tightly fixed to the support member. The support member can be a support ring 220, and the support ring 220 is not strictly limited to a circular ring or a circumferentially closed ring. The rigid wire 211 is tightly fixed in the support ring 220. This embodiment takes a circumferentially closed circular ring as an example, which can obtain uniform mechanical properties in the circumferential direction and a larger area / perimeter ratio.

[0079] The support ring 220 can be a separate component installed in the housing 100, or can be a part of the housing 100 directly as the support ring 220. For the same cutting element, at least a part is a working area for cutting and filtering fat, that is, the rigid wire 211 is exposed to the working area. The working area can be continuously distributed, that is, the working area is the rigid wire 211, or the working area is spaced apart, that is, the rigid wire is distributed in multiple areas, and the adjacent working areas are reinforced parts fixed to the support ring 220. Not only can the structural strength be ensured, but different working areas can also be designed to be in different flow channels, that is, the fat tissue passes through different working areas at different stages. The outer periphery of the support ring 220 is in sealing cooperation with the inner wall of the housing 100, so as to ensure that the fat tissue flows through the working area.

[0080] For the weaving principle of the rigid wire mesh 210, the related art can be combined. In some embodiments, the weaving method adopted is plain weaving or twist weaving. Plain weaving is a weaving method in which warp and weft are staggered. After being tightened, the warp and weft overlap each other to limit the position of the intersection of the warp and weft, so as to maintain a stable mesh shape and size. Twist weaving also includes common warp knitting. Adjacent warp wires are woven two by two to form a grid structure. Through the rigid wire mesh 210, a larger porosity and a more uniform pore size can be obtained, so that the size of the cut fat tissue is more uniform. The weaving method is relatively simple to process, low in cost, and can ensure the cutting effect and implement efficient fat cutting.

[0081] Since the wire cutting method is adopted, the porosity of the entire rigid wire mesh 210 can be much higher than that of the cutting and filtering components of the related art. In the present application, the porosity of the rigid wire mesh 210 can be greater than or equal to 80%. Further, it can be greater than or equal to 90%.

[0082] In one embodiment, in order to balance the structural strength of the rigid wire mesh 210 and the cutting and filtering effect on the fat tissue, the wire diameter of the rigid wire mesh 210, that is, the diameter of the rigid wire 211, is 0.05 mm to 1 mm. In one embodiment, the diameter of the rigid wire 211 is 0.05 mm to 0.5 mm.

[0083] Related to this is the pore size of the rigid wire mesh 210, that is, the size of the mesh hole 212. In one embodiment, the size of the mesh hole 212 is 300 μm to 3000 μm. In one embodiment, the size of the mesh hole 212 is 300 μm to 2500 μm.

[0084] The cross-sectional shape of the rigid wire 211 can be circular, oval, or rectangular. When the cross-section is non-circular, the wire diameter in the above can be understood as the diameter of an equivalent circular shape.

[0085] The shape of the mesh hole 212 is polygonal, such as triangular, quadrilateral (for example, parallelogram, and for example, rectangular or rhombic), hexagonal, and the like. The hole diameter of the mesh hole 212 in the foregoing can be understood as the side length of an equilateral square.

[0086] In some embodiments below, further optimization is made for the diameter of the rigid wire 211 and the hole diameter of the mesh hole 212, which can take into account the cutting and filtering effects, and reduce the blockage, for example, the ratio of the hole diameter of the rigid wire mesh 210 to the wire diameter is 8:1-1:1. In some embodiments, the wire diameter of the rigid wire mesh 210 is 0.15mm-0.50mm, and the ratio of the hole diameter of the rigid wire mesh 210 to the wire diameter is 5:1-1:1.

[0087] In some embodiments, the diameter of the rigid wire 211 is 0.15mm-0.30mm, and the hole diameter of the mesh hole 212 is 500μm-1500μm.

[0088] Referring to FIG. 3, in an embodiment, the cutting member 200 is a layer, which can be clamped and positioned in the shell 100 in combination with the positioning member 122 in the shell 100. The positioning member 122 can be a plurality of positioning columns or the like, and if necessary, positioning holes can be formed on the support ring 220 for the positioning member 122 to pass through to facilitate cooperation with each other.

[0089] Referring to FIGS. 5-7, in an embodiment, the cutting member 200 is a plurality of layers arranged at intervals, i.e., a plurality of rigid wire meshes, which can further improve the fat utilization rate, increase the product yield, and reduce the free oil and free water content, and the target size performance after cutting is better. The multi-layer cutting member 200 can be kept in a relative positional relationship by the retainer 300 and in combination with the positioning member 122 in the shell 100, and can also be directly processed as a pre-assembled body, i.e., the multi-layer cutting member is pre-fixed to each other by the respective support rings 220, and then the whole is packaged in the shell 100.

[0090] When a multi-layer (for example, 2-6 layers) structure is used, the hole diameters of the respective rigid wire meshes 210 are arranged in sequence, which can make the biological index performance of the product more excellent, the simulation clinical performance more optimal, the fat stem cell content higher, and the total cell number and cell survival rate higher. For example, as shown in the figure, three layers of rigid wire meshes arranged at intervals are provided, which are a first cutting member 200a, a second cutting member 200b, and a third cutting member 200c, and the hole diameters of the three layers are sequentially smaller, and the wire diameters can also be sequentially smaller. When used, the adipose tissue can be cut and filtered through the mesh holes from large to small in sequence to obtain the required cutting product.

[0091] When a multi-layer rigid wire mesh is used, in order to improve the shunt and cutting effect, the mesh hole shapes of the two adjacent rigid wire meshes can be different, for example, one is square and the other is hexagonal, and on this basis, further combination of different hole diameters and / or wire diameters can be made.

[0092] Referring to FIG. 8, taking two adjacent rigid wire meshes as an example, the pore size and wire diameter of the first cutting member 200a are both larger than those of the second cutting member 200b. Moreover, the rigid wires of the two adjacent rigid wire meshes can have different extension trends, for example, the extension trends of the rigid wires of the first cutting member 200a and the second cutting member 200b have an included angle of about 45°, and under the viewing angle in the figure, the two rigid wires are obliquely intersected to form a complex spatial network.

[0093] As shown in FIG. 8, the rigid wire mesh includes warp lines 210a and weft lines 210b that are perpendicularly intersected, and the extension directions of the warp lines 210a (or the weft lines 210b) of the two adjacent rigid wire meshes have an included angle of 30°-60°. That is, along the first direction, the included angle of the orthographic projection of the warp lines 210a (or the weft lines 210b) of the two adjacent rigid wire meshes is 30°-60°. In the system of multiple rigid wire meshes, the mesh holes are arranged staggeredly, which can further change the flow form of the fat tissue to be cut and can more efficiently cut the fat.

[0094] Research shows that, in combination with the application scenarios of the present application, the spacing between the two adjacent meshes has certain influence on the cutting effect and the cutting filtration resistance. Taking the first cutting member 200a and the second cutting member 200b as an example in combination with FIG. 9, the pore size of one is defined as D1, the pore size of the other is defined as D2, and the spacing therebetween is defined as L, and D1, D2 and L satisfy the following relationship: 0.5(D1+D2)≤L≤6(D1+D2). In some embodiments, D1, D2 and L satisfy the following relationship: (D1+D2)≤L≤1.5(D1+D2). The appropriate spacing can not only obtain better fluid distribution but also reduce blockage.

[0095] Based on the above embodiments, an embodiment of the present application further provides a processing method of a fat cutting filter, which includes: providing a rigid wire, pre-encoding the rigid wire to obtain a rigid wire mesh; providing a support ring, cutting the rigid wire mesh according to a predetermined shape, and tightly fixing the cut rigid wire mesh on the support ring, for example, the support ring can be distributed in pairs, and the same pair of support rings clamps and fixes the edges of the rigid wire mesh; providing a shell, the shell has a first interface and a second interface, and the cutting member is encapsulated in the shell and located between the first interface and the second interface.

[0096] Referring to FIG. 10, an embodiment of the present application further provides a fat cutting and filtering device, which includes the fat cutting and filtering filter of the above embodiments and a driving device 400, the driving device 400 is in communication with the first interface and the second interface on the shell 100 through a pipeline 410, and drives the fat tissue to be processed to pass through the cutting member.

[0097] The driving device 400 can adopt various modes such as a manual pump or an electric pump. In the embodiment, the driving device 400 is a set, which drives the fat tissue to pass through the fat cutting filter in a single flow direction. Of course, according to the type of the driving device 400, the fluid can be reversed.

[0098] Referring to FIG. 11, the application also provides a fat cutting filter device in an embodiment. The difference from the above embodiments is that the driving device 400 is two sets, which are a first pump body 420 and a second pump body 430, for example, a manual syringe and the like. The two sets of driving devices 400 are alternately pushed to make the fat tissue pass through the fat cutting filter in a bidirectional reciprocating manner. The fat cutting is completed in another syringe after the reciprocating pushing for multiple times (odd times).

[0099] Based on the above embodiments, the application also provides a fat cutting filtering method in an embodiment, which includes: driving the fat tissue to be processed to pass through a predetermined channel, which can be understood as a channel inside the shell and connected between the first interface and the second interface; arranging a rigid wire in the predetermined channel in advance, and cutting the fat tissue through the rigid wire. The rigid wire can be arranged in the predetermined channel in various combinations, for example, the rigid wire mesh adopted in the above embodiments. Along the flow direction of the fat tissue, the rigid wire includes one or more units. In the same unit (i.e., corresponding to a layer of rigid wire mesh), the rigid wires are arranged alternately and constitute a mesh hole allowing the fat tissue to pass through. The wire diameter and the hole diameter of the rigid wire can be combined with the above embodiments.

[0100] In some embodiments, the predetermined channel can also be a channel arranged between a plurality of fat cutting filters.

[0101] After passing through the fat cutting filter, the fat tissue is cut into uniform fat particles, and the free oil can be controlled within 10%. According to the cutting filter mesh with a single different hole diameter or the combination thereof, the free oil and free water can be controlled at different levels to meet different clinical demand scenarios (the treatment of osteoarthritis needs to be controlled below 5%, and the medical filling can be appropriately relaxed to below 10%).

[0102] The total number of cells in the fat tissue prepared by the fat cutting filter of the application can reach 1x10 6 cells / mL or more, the cell survival rate can reach 60% or more, and the fat stem cell content can reach 40% or more.

[0103] In addition, the free oil, free water, and the total number of cells, cell survival rate, and fat stem cell content can be regulated by the arrangement and combination of cutting filter meshes with different hole diameters, such as the combination of 1500 μm and 1000 μm or the combination of 1500 μm, 1000 μm, and 500 μm.

[0104] Further provided below are multiple embodiments and experimental examples to illustrate the use of the fat cutting filter of the present application and the corresponding effects.

[0105] Embodiment 1

[0106] The fat tissue is cut through the fat cutting filter with only one cutting member (as shown in Fig. 3) in the manner shown in Fig. 9, and the fat tissue is repeatedly pushed through the fat cutting filter by a syringe for cutting and filtering.

[0107] Embodiment 2

[0108] The fat tissue is cut through multiple fat cutting filters in sequence, and each fat cutting filter has only one cutting member (as shown in Fig. 3), but the pore sizes of the rigid wire meshes in the multiple fat cutting filters are arranged in sequence from large to small.

[0109] Embodiment 3

[0110] The fat tissue is cut through the same fat cutting filter with multiple cutting members (as shown in Fig. 5), and the pore sizes of the rigid wire meshes are arranged in sequence from large to small, and the fat tissue is repeatedly pushed through the fat cutting filter by a syringe for cutting and filtering.

[0111] Embodiment 4

[0112] The fat cutting filter of Embodiment 1 is changed in wire diameter and pore size of the rigid wire mesh, and the cut fat tissue is tested in free oil and free water content.

[0113] Experimental Example 1: Test the fat particle size of different samples

[0114] The fat particle tissue is dispersed in a phosphate buffer solution (PBS) and then loaded into a sample tank for particle size analysis, and the particle size distribution result is obtained by laser scattering particle size distribution testing, as shown in Fig. 12. The symbols in the figure are as follows:

[0115] Embodiment 1: The fat cutting filter described in Embodiment 1 is used to cut fat, and the wire diameter of the rigid wire mesh is 0.3-0.5 mm and the pore size is 1500 μm.

[0116] Embodiment 2: The multiple fat cutting filters described in Embodiment 2 are used to cut fat, and the pore sizes of the rigid wire meshes of the fat cutting filters are 1500 μm, 1000 μm and 500 μm in sequence, and the wire diameters are 0.3-0.5 mm, 0.2-0.3 mm and 0.05-0.2 mm in sequence.

[0117] Example 3: fat was cut by the fat cutting filter as described in Example 3 above, the pore size of each layer of rigid wire mesh was 1500 μm, 1000 μm and 500 μm, and the wire diameter was 0.3-0.5 mm, 0.2-0.3 mm and 0.05-0.2 mm, respectively.

[0118] Non-mesh: a commercially available non-mesh cutting filter (pore size of 500-600 μm) was used.

[0119] Test Example 2: test the free oil, free water content and SVF yield in different samples

[0120] The fat cutting filters of Examples 1-3 above were used to cut fat, respectively, and the test results of free oil, free water content in each sample after cutting are shown in Figure 13, and the test results of SVF yield are shown in Figure 14, wherein.

[0121] The pore size of the cutting filter mesh (i.e. rigid wire mesh) of Example 1 was 500 μm.

[0122] Example 2 used three single-layer fat cutting filters, and the pore size of the cutting filter mesh was 1500 μm, 1000 μm and 500 μm, respectively.

[0123] Example 3 used the same fat cutting filter with three layers of cutting filter mesh, and the pore size of the three layers of cutting filter mesh was 1500 μm, 1000 μm and 500 μm, respectively.

[0124] The wire diameter was controlled at 0.15 mm.

[0125] Test Example 3: total cell number per unit volume, cell survival rate, adipose-derived stem cells in SVF product

[0126] Trypan blue staining: live cells will not be stained blue by trypan blue, while dead cells will be stained light blue. After trypan blue staining, the cell survival rate can be quantitatively compared by direct counting under a microscope or counting after taking pictures under a microscope.

[0127] Taking a fat cutting filter with only one cutting piece as an example, the total cell number per unit volume and cell survival rate in the SVF product obtained after cutting were tested, and the results are shown in Table 1.

[0128] Table 1

[0129] Table 2 shows the results of cell biology tests of SVF products prepared by using the fat cutting filters described in the above examples (wherein the wire diameter of the rigid wire mesh was 0.15 mm).

[0130] Table 2

[0131] Any combination of the technical features in the above-described embodiments can be made, and for the sake of brevity, not all possible combinations are described, however, it is to be understood that the application encompasses all such possible combinations. Technical features in different embodiments can be embodied in the same figure, and it can be considered that the figure also discloses a combination of the embodiments involved.

[0132] The above-described embodiments only express several implementation manners of the present application, and the description is relatively specific and detailed, but it should not be understood as a limitation on the patent application scope. It should be noted that, for ordinary skilled persons in the art, several modifications and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the patent protection scope of the present application should be subject to the appended claims.

Claims

1. A fat-cut filter, characterized by, The shell comprises a processing cavity in the interior, a first interface and a second interface communicating with the processing cavity; The cutting member is located in the processing cavity and between the first interface and the second interface; the cutting member comprises a rigid wire mesh for cutting fat, and the wire diameter of the rigid wire mesh is 0.05mm-1mm. The ratio of the aperture diameter to the wire diameter of the rigid wire mesh is 8:1-1:

1.

2. The fat-cutting filter according to claim 1, wherein, The wire diameter of the rigid wire mesh is 0.15mm-0.50mm, and the ratio of the aperture diameter to the wire diameter of the rigid wire mesh is 5:1-1:

1.

3. The fat-cutting filter of claim 1, wherein, The rigid wire mesh is a woven mesh, the weaving mode of the woven mesh is plain weaving or twist weaving, and the mesh hole shape of the rigid wire mesh is polygonal.

4. The fat-cutting filter of claim 1, wherein, The cutting member comprises one layer of rigid wire mesh or multiple layers of rigid wire mesh arranged at intervals.

5. Fat-cut filter according to any one of claims 1-4, characterized in that, The cutting member comprises multiple layers of rigid wire mesh arranged at intervals along a first direction, and adjacent two layers of the multiple layers of rigid wire mesh adopt at least one of the following configuration modes:

6. The fat-cutting filter according to any one of claims 1 to 4, wherein, a) the mesh hole shape of each rigid wire mesh is different; b) the aperture diameter of each rigid wire mesh is different; c) the wire diameter of each rigid wire mesh is different; d) along the first direction, the rigid wire of each rigid wire mesh extends in different directions. Along the first direction, the aperture diameter of each layer of the multiple layers of rigid wire mesh gradually decreases, and the wire diameter of each layer of the multiple layers of rigid wire mesh gradually decreases.

7. The fat-cutting filter according to claim 6, wherein, The aperture diameter of one of the adjacent two layers of the rigid wire mesh is defined as D1, the aperture diameter of the other is defined as D2, and the distance between the adjacent two layers of the mesh is defined as L, D1, D2 and L satisfy the following relationship: 0.5(D1+D2)≤L≤3(D1+D2).

8. The fat-cutting filter of claim 6, wherein, The number of layers of the rigid wire mesh is 2-6.

9. The fat-cutting filter according to claim 5 or 6, wherein, The cross-sectional shape of the rigid wire is circular, elliptical or rectangular.

10. The fat-cutting filter of claim 1, wherein, The shell further comprises a positioning member for clamping the cutting member.

11. The fat-cutting filter of claim 1, wherein, The cutting member comprises:

12. The fat-cutting filter of claim 1, wherein, At least a part of the cutting member is a working area for cutting and filtering fat, and the porosity of the working area is greater than or equal to 80%. The cutting member comprises:

13. The fat-cutting filter of claim 12, wherein, A support ring, which is configured separately or is part of the shell; A rigid wire mesh fixed in the support ring and providing the working area, the wire diameter of the rigid wire mesh is 0.15mm-0.30mm, and the aperture diameter is 500μm-1500μm. The fat cutting filter comprises the fat cutting filter according to any one of claims 1-13; 14. A fat-cutting filter apparatus, characterized by, A driving device communicating with the first interface and the second interface, and driving the fat tissue to be processed to pass through the cutting member. The driving device comprises: Driving the fat tissue to be processed to pass through a predetermined channel; 15. A fat-cutting filtration method, characterized by, A rigid wire mesh is arranged in the predetermined channel in advance, and the fat tissue is cut by the wire of the rigid wire mesh according to any one of claims 1-14, and the wire diameter of the rigid wire mesh is 0.05mm-1mm; Along the flow direction of the fat tissue, the rigid wire mesh is one or multiple layers of rigid wire meshes arranged at intervals. ​ ​

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