Composition of natural product lime essential oil and silver nitrate, and use thereof

Abstract

A composition of the natural product lime essential oil and silver nitrate. The use of high-concentration lime essential oil alone has an inhibitory effect on Escherichia coli BW25113, but the inhibitory effect on Escherichia coli BW25113 of AgNO3 used along is not significant. By means of combining the natural product lime essential oil with silver nitrate, it is found that the bactericidal effect on Escherichia coli BW25113 is significant, and the synergistic effect of lime essential oil and silver nitrate can reduce the use of high-concentration lime essential oil while ensuring the bactericidal effect. The composition can replace the use of antibiotics while exerting bacteriostatic effects more efficiently. The described effect is also found to be applicable for inhibiting Staphylococcus aureus and drug-resistant Escherichia coli. In addition, a deodorization effect against aqueous ammonia indicates that the combination of lime essential oil and silver nitrate enhances a deodorization effect, the usage amount of the two components is low, it is safe and effective, the raw materials are easily available, and there are relatively good application prospects in the preparation of antibacterial and deodorizing products.

Description

Composition of natural product lime essential oil and silver nitrate and application thereof TECHNICAL FIELD

[0001] The present application belongs to the technical field of bacteriostatic and deodorant products, and specifically discloses a composition of natural product lime essential oil and silver nitrate and application thereof. BACKGROUND

[0002] Lime essential oil is distilled from lime berries. The smell is quite strong, bitter and sweet, and yellowish. The main components of lime essential oil are terpinene, coriander oil alcohol, and terpineol, which have the effects of anti-anaemia, antibacterial, and antiviral.

[0003] Some studies have shown that due to different mechanisms of different essential oil components in playing a bacteriostatic effect, the combined use of multiple essential oil components can play a synergistic effect, and antagonism may also occur. The development of composite preparations of essential oils combined with other types has become a major research direction. Silver ions can strongly attract the sulfhydryl group (-SH) on the protease in the bacterial body, rapidly combine with it, make the protease lose activity, and cause the death of bacteria. At present, the state is committed to the implementation of the policy of reducing the use of antibiotics, and there are not many reported synergistic studies of essential oils combined with compounds for sterilization. Therefore, the research on compounds that can have a combined bactericidal effect with lime essential oil has good application prospects.

[0004] In addition, environments that need to be disinfected, such as livestock, factories, sewage treatment, landfills, hospitals, and the like, also face the need for deodorization. However, the existing chemical deodorants have high use cost, large use amount, and high environmental risk. Therefore, the development of green, low-risk, and low-cost deodorants also has strong practical significance. TECHNICAL PROBLEM

[0005] The purpose of the present application is to overcome the defects and deficiencies existing in the prior art, and to provide a combination of natural product lime essential oil and silver nitrate.

[0006] The second purpose of the present application is to provide the use of the above combination in the field of bacteriostasis and deodorization. TECHNICAL SOLUTION

[0007] The purpose of the present application is achieved by the following technical solutions:

[0008] A bacteriostatic and deodorizing composition is composed of lime essential oil and silver nitrate.

[0009] Preferably, in the essential oil compound, the concentration of lime essential oil is 1%-5%, and the concentration of silver nitrate is 0.25-0.5 μg / mL.

[0010] The present application also provides the use of the bacteriostatic and deodorizing composition in the preparation of bacteriostatic and deodorizing products.

[0011] Preferably, the bacteriostatic object of the product is foodborne bacteria, such as Escherichia coli or / and Staphylococcus aureus.

[0012] More preferably, the Escherichia coli is drug-resistant Escherichia coli.

[0013] Preferably, the deodorizing object of the product is ammonia or aqueous ammonia.

[0014] Preferably, the product is in the form of a solid, a liquid, a pump spray or a semi-solid gel.

[0015] The application also provides a bacteriostatic and deodorizing product comprising the bacteriostatic and deodorizing composition.

[0016] Preferably, the solvent of the bacteriostatic and deodorizing product is water.

[0017] More preferably, the concentration of the lime essential oil is 1% to 5%. The above-mentioned deodorizing product is preferably in the form of a solution, which can be directly sprayed for convenient use. Advantages

[0018] Compared with the prior art, the application has the following advantages:

[0019] The application provides a combination of the natural product lime essential oil and silver nitrate, and it is found that the combination of lime essential oil and silver nitrate has a significant bactericidal effect on Escherichia coli BW 25113. The synergy of lime essential oil and silver nitrate can reduce the use of high-concentration lime essential oil while ensuring the bactericidal effect. The above-mentioned effect is also applicable to the inhibition of Staphylococcus aureus and drug-resistant Escherichia coli. The composition of the application has a more efficient bacteriostatic effect and can replace the use of antibiotics. The deodorizing effect on aqueous ammonia shows that the combination of lime essential oil and silver nitrate enhances the deodorizing effect, the use amount of the two components is low, it is safe and effective, the raw materials are easy to obtain, and it has a good application prospect in the preparation of bacteriostatic and deodorizing products. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 is a statistical result graph of antibacterial in Example 2.

[0021] Figure 2 is a statistical result graph of antibacterial in Example 3.

[0022] Figure 3 is a statistical result graph of antibacterial in Example 4. BEST MODE FOR CARRYING OUT THE INVENTION

[0023] The following specific embodiments of the application will fully and comprehensively describe the technical solutions of the application. It should be pointed out that the provided embodiments only represent a part of the application, not all. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the application.

[0024] The test method used in the embodiments of the present application is a conventional method unless otherwise specified; the materials, reagents, etc. used are commercially available reagents and materials unless otherwise specified, and the commercially available raw materials are used after removing insoluble impurities, etc. by a general filtration method.

[0025] The lemongrass essential oil (LM) used is a product of Guangzhou Wenyuan Essential Oil Co., Ltd.; and the silver nitrate (AgNO3) is a product of the brand of Macklin, MW 169.87.

[0026] Determination principle: if the reduced bacterial amount of the experimental group is reduced by more than 0.5 log value on the basis of the bacterial amount reduction of the lemongrass essential oil treatment alone and the silver nitrate treatment alone, it indicates that the lemongrass essential oil and the silver nitrate have a synergistic antibacterial effect.

[0027] Example 1 MIC determination of lemongrass essential oil and silver nitrate on Escherichia coli BW 25113 and Staphylococcus aureus ATCC 29213

[0028] 1. Experimental materials:

[0029] (1) Test: cool the high-pressure sterilized MH broth for standby use.

[0030] (2) Lemongrass essential oil: Guangzhou Wenyuan Essential Oil Co., Ltd.; silver nitrate: Macklin brand, MW 169.87.

[0031] Test strains: Escherichia coli wild strain BW 25113; Staphylococcus aureus quality control strain ATCC 29213.

[0032] 2. Preparation work before the test:

[0033] (1) Subculture Escherichia coli wild strain BW 25113 and Staphylococcus aureus quality control strain ATCC 29213 on agar medium, and culture to an appropriate size.

[0034] (2) Weigh 0.1 g of silver nitrate powder in a centrifuge tube, add 10 mL of sterile water to dissolve, the silver nitrate concentration is 10 mg / mL, vortex to mix and filter, and the filtrate is physically sterilized through a filter membrane with a diameter of 0.22 μm, and is divided into sterilized centrifuge tubes and stored in a -20℃ refrigerator.

[0035] 3. MIC determination experiment:

[0036] (1) Inoculate Escherichia coli BW 25113 and Staphylococcus aureus ATCC 29213 in a centrifuge tube containing 4 mL of MH broth, and place it in a 37℃ shaking bed at 180 rpm for 4 h, then take out the centrifuge tube;

[0037] (2) Dilute the incubated bacteria to 100 times, about 10 6 CFU / mL, standby;

[0038] (3) Take a sterile 96-well plate, add 180 μL of MH broth medium to the first well, and add 100 μL of MH broth medium to the second to twelfth wells;

[0039] (4) Add 20 μL of lime essential oil or 20 μL of silver nitrate (640 μg / mL) to the first well, mix evenly, and then suck 100 μL into the second well, and so on. Suck 100 μL from the tenth well and discard;

[0040] (5) Add 100 μL of diluted bacteria to the first to eleventh wells, and add 200 μL of MH broth to the twelfth well;

[0041] (6) The final concentration of lime essential oil in the 1-10 wells of the 96-well plate is 5%, 2.5%, 1.25%, 0.625%, 0.3125%, 0.156%, 0.078%, 0.039%, 0.0195%, and 0.00975%, respectively. The eleventh well is a growth control, and the twelfth well is a blank control;

[0042] (7) The final concentration of silver nitrate in the 1-10 wells of the 96-well plate is 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125, and 0.0625 μg / mL, respectively. The eleventh well is a growth control, and the twelfth well is a blank control;

[0043] (8) Set 3 rows of repeats for each drug;

[0044] (9) Place the inoculated 96-well plate in a 37°C incubator for 16-18 hours, and read the results.

[0045] The results are shown in Table 1. The MIC value of lime essential oil for BW 25113 is >5%, and the MIC value of silver nitrate is 2 μg / mL. The MIC value of lime essential oil for ATCC 29213 is >5%, and the MIC value of silver nitrate is 2 μg / mL.

[0046] Table 1 MIC of lime essential oil and silver nitrate on Escherichia coli and Staphylococcus aureus

[0047] Example 2 Evaluation of the combined bactericidal effect of lime essential oil and silver nitrate on Escherichia coli BW 25113

[0048] 1. Experimental materials:

[0049] (1) Test: Cool the high-pressure sterilized LB broth, PBS buffer, and ultrapure water for standby use.

[0050] ((2) Lemon essential oil: Guangzhou Yuyuan Essential Oil Co., Ltd.; Silver nitrate: Macklin brand, MW 169.87

[0051] Test strain: Escherichia coli wild strain BW 25113 (laboratory preservation).

[0052] 2. Preparation before the test:

[0053] (1) The wild strain of Escherichia coli BW 25113 was subcultured on agar medium and cultured to an appropriate size.

[0054] (2) 0.1 g of silver nitrate powder was weighed on a precision balance in a centrifuge tube, 10 mL of sterile water was added for dissolution, the silver nitrate concentration was 10 mg / mL, vortex mixing was performed, and filtration was performed. The filtrate was physically sterilized through a filter membrane with a diameter of 0.22 μm, and was divided into sterilized centrifuge tubes and stored in a -20°C refrigerator.

[0055] 3. Bactericidal experiment:

[0056] (1) Inoculate Escherichia coli BW 25113 single colony in a centrifuge tube containing 20 mL of LB broth, and place it in a 37°C shaking bed at 180 rpm for 3 h. Then take out the centrifuge tube.

[0057] (2) Centrifuge the cultured bacterial solution at 5500 rpm for 3 min, resuspend with an equal volume of PBS buffer, and dilute 10 times. The bacterial amount is 10 7 CFU / mL, ready for use.

[0058] (3) The system used in this experiment is 1 mL, and the bacterial solution is the bacterial solution in (2):

[0059] A) Control group: 0.7 mL PBS + 0.2 mL PBS + 0.1 mL bacterial solution;

[0060] B) Single drug group: 0.7 mL PBS + 0.1 mL A / B drug + 0.1 mL PBS + 0.1 mL bacterial solution;

[0061] C) Combined group: 0.7 mL bacterial solution + 0.1 mL A drug + 0.1 mL B drug + 0.1 mL bacterial solution.

[0062] (4) This test sets up 9 groups:

[0063] E. coli BW25113: ① control group; ② single lemon essential oil group: 1 / 2 MIC (2.5%) lemon essential oil; ③ single lemon essential oil group: 1 / 4 MIC (1.25%) lemon essential oil; ④ single silver nitrate group: 0.25 μg / mL silver nitrate; ⑤ single silver nitrate group: 0.5 μg / mL silver nitrate; ⑥ combination group: 1 / 4 MIC lemon essential oil + 0.25 μg / mL silver nitrate; ⑦ combination group: 1 / 4 MIC lemon essential oil + 0.5 μg / mL silver nitrate; ⑧ combination group: 1 / 2 MIC lemon essential oil + 0.25 μg / mL silver nitrate; ⑨ combination group: 1 / 2 MIC lemon essential oil + 0.5 μg / mL silver nitrate;

[0064] (5) After the system is prepared, it is cultured at 37°C, 180 rpm shaking bed for 1h.

[0065] (6) At 1h, 100 μL of bacterial solution is taken from each tube and added to a 2ml centrifuge tube containing 900 μL of PBS buffer for ten-fold gradient dilution. After dilution, 25 μL is taken and dropped on LB agar medium, which is incubated in a 37°C incubator for 16-18h for colony counting. The experimental results are statistically analyzed after three biological repeats.

[0066] The results are shown in Table 2 and Figure 1. In the blank control group, the bacteria grew normally, indicating that E. coli BW25113 could grow normally under the experimental conditions. In the single drug group, E. coli BW25113 did not significantly decrease compared with the single AgNO3 group, indicating that the single AgNO3 group had no obvious inhibitory effect on E. coli BW25113. Similarly, the results also showed that the use of high-concentration lemon essential oil alone had a significant inhibitory effect on E. coli BW25113. The results of the combination group showed that the synergistic effect of lemon essential oil and silver nitrate had a significant bactericidal effect on E. coli BW25113.

[0067] Table 2 Bactericidal effect of lemon essential oil and silver nitrate combination on E. coli wild strain BW25113

[0068] Example 3 Evaluation of the combined bactericidal effect of lemon essential oil and silver nitrate on Staphylococcus aureus ATCC 29213

[0069] 1. Experimental materials:

[0070] (1) Test: LB broth, PBS buffer, and ultrapure water sterilized by high pressure were cooled for standby.

[0071] (2) Lemon essential oil: Guangzhou Wenyuan Essential Oil Co., Ltd.; Silver nitrate: brand of MacLin, MW 169.87

[0072] Test strain: Staphylococcus aureus ATCC 29213 (laboratory preservation).

[0073] 2. Preparation before the test:

[0074] (1) Staphylococcus aureus ATCC 29213 was subcultured on agar medium and cultured to an appropriate size.

[0075] (2) 0.1 g of silver nitrate powder was weighed on a precision balance in a centrifuge tube, 10 mL of sterile water was added for dissolution, the silver nitrate concentration was 10 mg / mL, vortex mixing was performed, and filtration was performed. The filtrate was physically sterilized through a filter membrane with a diameter of 0.22 μm, and was divided into sterilized centrifuge tubes and stored in a -20°C freezer.

[0076] 3. Bactericidal experiment:

[0077] (1) Staphylococcus aureus ATCC 29213 single colony was inoculated in a centrifuge tube containing 20 mL of LB broth, and was placed in a 37°C shaker at 180 rpm for 3 h. The centrifuge tube was removed.

[0078] (2) The cultured bacterial solution was centrifuged at 5500 rpm for 3 min, and was resuspended with an equal volume of PBS buffer and diluted 10 times. The bacterial amount was 10 7 CFU / mL, ready for use.

[0079] (3) The system used in this experiment was 1 mL, and the bacterial solution was added as follows:

[0080] A) Control group: 0.7 mL PBS + 0.2 mL PBS + 0.1 mL bacterial solution;

[0081] B) Single drug group: 0.7 mL PBS + 0.1 mL A / B drug + 0.1 mL PBS + 0.1 mL bacterial solution;

[0082] C) Combined group: 0.7 mL bacterial solution + 0.1 mL A drug + 0.1 mL B drug + 0.1 mL bacterial solution.

[0083] (4) This test set up 9 groups:

[0084] Staphylococcus aureus ATCC 29213: ① control group; ② single lemon essential oil group: 1 / 2 MIC (2.5%) lemon essential oil; ③ single lemon essential oil group: 1 / 4 MIC (1.25%) lemon essential oil; ④ single silver nitrate group: 0.25 μg / mL silver nitrate; ⑤ single silver nitrate group: 0.5 μg / mL silver nitrate; ⑥ combination group: 1 / 4 MIC lemon essential oil + 0.25 μg / mL silver nitrate; ⑦ combination group: 1 / 4 MIC lemon essential oil + 0.5 μg / mL silver nitrate; ⑧ combination group: 1 / 2 MIC lemon essential oil + 0.25 μg / mL silver nitrate; ⑨ combination group: 1 / 2 MIC lemon essential oil + 0.5 μg / mL silver nitrate;

[0085] (5) After the system is prepared, it is cultured at 37°C for 1 h with 180 rpm shaking bed.

[0086] (6) At 1 h, 100 μL of bacterial solution is taken from each tube and added to a 2 ml centrifuge tube containing 900 μL of PBS buffer for ten-fold gradient dilution. After dilution, 25 μL is taken and dropped on LB agar medium, which is incubated in a 37°C incubator for 16-18 h for colony counting. The experimental results are statistically analyzed after three biological repetitions.

[0087] The results are shown in Table 3 and FIG. 2. In the blank control group, the bacteria grew normally, indicating that Staphylococcus aureus ATCC 29213 could grow normally under the experimental conditions. In the single drug group, Staphylococcus aureus ATCC 29213 did not significantly decrease compared with the single AgNO3 group, indicating that the single AgNO3 group had no obvious inhibitory effect on Staphylococcus aureus ATCC 29213. Similarly, the results also showed that the single use of lemon essential oil had an obvious inhibitory effect on Staphylococcus aureus ATCC 29213. The results of the combination group showed that the synergistic effect of lemon essential oil and silver nitrate had a significant bactericidal effect on Staphylococcus aureus ATCC 29213.

[0088] Table 3 Bactericidal effect of lemon essential oil and silver nitrate combination on Staphylococcus aureus quality control strain ATCC 29213

[0089] Example 4 Evaluation of the combined bactericidal effect of lemon essential oil and silver nitrate on multi-drug resistant Escherichia coli NP109

[0090] 1. Experimental materials:

[0091] (1) Test: Cool the high-pressure sterilized LB broth, PBS buffer, and ultrapure water for standby use.

[0092] (2) Lemon essential oil: Guangzhou Wenyuan Essential Oil Co., Ltd.

[0093] Silver nitrate: brand of McKin, MW169.87

[0094] Test strain: Escherichia coli multi-drug resistant bacteria NP109 (laboratory preservation).

[0095] 2, Preparation before the test:

[0096] (1) Escherichia coli multi-drug resistant bacteria NP109 was subcultured on agar medium and cultured to the appropriate size.

[0097] (2) 0.1 g of silver nitrate powder was weighed on a precision balance in a centrifuge tube, 10 mL of sterile water was added for dissolution, the concentration of silver nitrate was 10 mg / mL, vortex mixing was performed, and the filtrate was filtered through a filter membrane with a diameter of 0.22 μm for physical sterilization, and was divided into sterile centrifuge tubes and stored in a -20℃ freezer.

[0098] 3, Bactericidal experiment:

[0099] (1) Escherichia coli multi-drug resistant bacteria NP109 single colony was inoculated in a centrifuge tube containing 20 mL of LB broth and placed in a 37℃ shaking bed at 180 rpm for 3h, then the centrifuge tube was taken out.

[0100] (2) The cultured bacterial solution was centrifuged at 5500 rpm for 3 min, resuspended with PBS buffer solution in equal volume and diluted 10 times, the bacterial amount was 10 7 CFU / mL, ready for use.

[0101] (3) The system used in this experiment was 1 mL, and the bacterial solution was added as follows:

[0102] A) Control group: 0.7 mL PBS + 0.2 mL PBS + 0.1 mL bacterial solution;

[0103] B) Single drug group: 0.7 mL PBS + 0.1 mL A / B drug + 0.1 mL PBS + 0.1 mL bacterial solution;

[0104] C) Combined group: 0.7 mL bacterial solution + 0.1 mL A drug + 0.1 mL B drug + 0.1 mL bacterial solution.

[0105] (4) This test sets 9 groups:

[0106] E. coli drug-resistant bacteria NP109: ① control group; ② single lemon essential oil group: 1 / 2 MIC (2.5%) lemon essential oil; ③ single lemon essential oil group: 1 / 4 MIC (1.25%) lemon essential oil; ④ single silver nitrate group: 0.25 μg / mL silver nitrate; ⑤ single silver nitrate group: 0.5 μg / mL silver nitrate; ⑥ combination group: 1 / 4 MIC lemon essential oil + 0.25 μg / mL silver nitrate; ⑦ combination group: 1 / 4 MIC lemon essential oil + 0.5 μg / mL silver nitrate; ⑧ combination group: 1 / 2 MIC lemon essential oil + 0.25 μg / mL silver nitrate; ⑨ combination group: 1 / 2 MIC lemon essential oil + 0.5 μg / mL silver nitrate;

[0107] (5) After the system is prepared, it is cultured at 37°C, 180 rpm shaking bed for 1h.

[0108] (6) At 1h, 100 μL of bacterial solution was taken from each tube and added to a 2ml centrifuge tube containing 900 μL of PBS buffer for ten-fold gradient dilution. After dilution, 25 μL was taken and dropped on LB agar medium, which was incubated in a 37°C incubator for 16-18h for colony counting. The experimental results were statistically analyzed after three biological repeats.

[0109] The results are shown in Table 4 and Figure 3. In the blank control group, the bacteria grew normally, indicating that under the experimental conditions, the E. coli multi-drug resistant bacteria NP109 could grow normally. In the single drug group, the E. coli multi-drug resistant bacteria NP109 did not significantly decrease compared with the single essential oil group, indicating that the single essential oil group had no obvious inhibitory effect on the E. coli multi-drug resistant bacteria NP109. Similarly, the results also showed that the use of AgNO3 alone had a significant inhibitory effect on the E. coli multi-drug resistant bacteria NP109. The results of the combination group showed that the synergistic effect of lemon essential oil and silver nitrate had a significant bactericidal effect on the E. coli multi-drug resistant bacteria NP109.

[0110] Table 4 Bactericidal effect of lemon essential oil and silver nitrate combination on E. coli multi-drug resistant bacteria NP109

[0111] Example 5 Deodorization effect evaluation of lemon essential oil combined with silver nitrate

[0112] 1. Experimental materials:

[0113] (1) Test: Prepare a portable gas detector and a closed aluminum box for standby.

[0114] (2) Lemon essential oil: Guangzhou Source Essential Oil Co., Ltd.; Silver nitrate: Macklin brand, MW 169.87

[0115] Test simulated farm odor: ammonia water.

[0116] 2. Preparation before the experiment:

[0117] (1) The portable gas detector was adjusted and self-checked.

[0118] 3. Deodorization experiment:

[0119] (1) Turn on the gas detector and place it in the detection box after adjustment. Wait for 3 minutes to ensure the stability of the instrument.

[0120] (2) The dose of ammonia used in this experiment is 10 μL, which is dropped on the fixed point of the detection box: control group: 10 μL of ammonia.

[0121] (3) This experiment sets 9 groups:

[0122] Ammonia: ① control group; ② single lime essential oil group: 1 / 2 MIC (2.5%) lime essential oil; ③ single lime essential oil group: 1 / 4 MIC (1.25%) lime essential oil; ④ single silver nitrate group: 0.25 μg / mL silver nitrate; ⑤ single silver nitrate group: 0.5 μg / mL silver nitrate; ⑥ combined drug group: 1 / 2 MIC lime essential oil + 0.25 μg / mL silver nitrate; ⑦ combined drug group: 1 / 2 MIC lime essential oil + 0.5 μg / mL silver nitrate; ⑧ combined drug group: 1 / 4 MIC lime essential oil + 0.25 μg / mL silver nitrate; ⑨ combined drug group: 1 / 4 MIC lime essential oil + 0.5 μg / mL silver nitrate;

[0123] (4) After liquid dropping, start detection and record the detection value every 3 minutes;

[0124] (5) Result determination: 0-19 is below the warning line, no alarm; 20-49 is low-level alarm; 50-100 is high-level alarm. (This value directly represents the concentration data value of ammonia degradation)

[0125] Table 5 Deodorization effect of lime essential oil and silver nitrate combined on ammonia

[0126] The results are shown in Table 5. The detection value of the blank control group is 100, indicating that the odor detection is stable under the experimental conditions. The results of single drug group and combined group show that the single and combined effects of lime essential oil and silver nitrate on ammonia deodorization are significant, but the combined effect on ammonia deodorization is better.

[0127] Obviously, the above embodiments of the present application are only examples for clearly illustrating the technical solutions of the present application, and are not intended to limit the specific embodiments of the present application. Any modification, equivalent replacement and improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A bacteriostatic deodorant composition characterized in that, The lime essential oil and silver nitrate.

2. The bacteriostatic deodorant composition of claim 1, wherein, The concentration of the lime essential oil in the essential oil complex is 1%-5%, and the concentration of the silver nitrate is 0.25-0.5 μg / mL.

3. Use of the bacteriostatic deodorant composition of claim 1 or 2 in the preparation of a bacteriostatic deodorant product.

4. Use according to claim 3, characterized in that, The bacteriostatic object of the product is Escherichia coli, multi-drug resistant Escherichia coli or / and Staphylococcus aureus.

5. Use according to claim 3, characterized in that, The deodorant object of the product is ammonia gas or ammonia water.

6. Use according to claim 3, characterized in that, The form of the product is solid, liquid, pump spray or semi-solid gel.

7. A bacteriostatic deodorant product characterized in that, The bacteriostatic deodorant composition of claim 1 or 2.

8. The bacteriostatic deodorant product of claim 7, wherein, The solvent of the bacteriostatic deodorant product is water.

9. The bacteriostatic deodorant product of claim 8, wherein, The concentration of the lime essential oil is 1-5%.

10. A method of bacteriostatic deodorization, characterized by, The bacteriostatic deodorant product of claim 9 is directly sprayed in the form of a dilute solution.

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