Clamp for transfer of biological tissue

Abstract

A clamp for the transfer of biological tissue. The clamp comprises: a first clamping portion, which has at least one carrier rod clamping slot, a carrier rod being gripped in the carrier rod clamping slot, and biological tissue is carried in an end of the carrier rod; and a second clamping portion, which has at least one cannula clamping slot, a cannula being gripped in the cannula clamping slot, wherein the end of the carrier rod is opposite an end of the cannula, and the first clamping portion moves in an axial direction, such that the biological tissue carried in the carrier rod is transferred into the cannula.

Description

Clamp for biological tissue transfer TECHNICAL FIELD

[0001] The utility model relates to the field of bioengineering technology, especially a clamp for biological tissue transfer. BACKGROUND

[0002] Although cryopreservation techniques, particularly vitrification, have achieved remarkable success in human medicine and are widely used in reproductive medicine, their application in the livestock industry has been greatly limited. This limitation is mainly influenced by factors such as cost, expertise, and ranch environment. First, vitrification requires expensive equipment and expertise, which may not be practical for the livestock industry. Specifically, embryos after thawing need to be transferred to recipient cows as soon as possible, and embryo technicians need to use mouth pipettes to transfer embryos in the carrier to the straw under the assistance of a microscope, which requires meticulous training and a professional laboratory environment. In the ranch environment, there is usually a lack of personnel with these professional skills, so it is difficult to apply this technology. In addition, ranches are usually located in remote areas, lacking the necessary infrastructure and equipment such as microscopes and freezing equipment, making the application of the technology more difficult.

[0003] In the prior art, embryo transfer requires skilled embryo technicians to operate, and after thawing, it needs to be transferred to the recipient cow as soon as possible, and the ranch environment is poor, which will lead to a decrease in the success rate of operation. In addition, the manual transfer and straw filling operation of the embryo is complicated, inefficient, and also causes unavoidable mistakes.

[0004] SUMMARY

[0005] The main purpose of the utility model is to provide a clamp for biological tissue transfer to solve the problems of complex manual embryo transfer technique operation and low success rate in the prior art.

[0006] According to the utility model embodiment, a clamp for biological tissue transfer is provided, which comprises: a first clamping part, the first clamping part has at least one carrier clamping groove, the carrier clamping groove clamps a carrier, and the end of the carrier carries biological tissue; a second clamping part, the second clamping part has at least one sleeve clamping groove, the sleeve clamping groove clamps a sleeve; wherein the end of the carrier and the end of the sleeve are opposite, the first clamping part moves along the axial direction, and the biological tissue carried in the carrier is transferred to the sleeve.

[0007] The clamp further comprises: a docking part, the docking part is detachably arranged between the first clamping part and the second clamping part, the docking part has at least one positioning through hole, the carrier passes through the positioning through hole so that the end of the carrier and the end of the sleeve are opposite.

[0008] The positioning through hole comprises a carrier rod positioning hole opened towards the first clamping part and a sleeve positioning hole opened towards the second clamping part, the carrier rod positioning hole and the sleeve positioning hole are communicated, and the carrier rod positioning hole and the sleeve positioning hole are gradually expanded from inside to outside in a trumpet shape.

[0009] An opening is arranged above the carrier rod positioning hole.

[0010] The clamp further comprises one or more carrier rod supporting frames, and each carrier rod supporting frame carries a plurality of carrier rods.

[0011] The first clamping part further comprises a locking structure for locking the carrier rod in the carrier rod clamping groove.

[0012] The clamp further comprises a sliding block arranged below the first clamping part, and the sliding block drives the first clamping part to move axially.

[0013] The height of the sleeve clamping groove and the carrier rod clamping groove is equal.

[0014] The carrier rod is provided with a groove near the front end, and the groove carries at least a liquid containing biological tissue.

[0015] The biological tissue comprises an embryo or an ovum.

[0016] According to the technical scheme of the utility model, the first clamping part for clamping the carrier rod carrying biological tissue and the second clamping part for clamping the sleeve are arranged, and the end of the carrier rod and the end of the sleeve are opposite to each other, when the first clamping part moves axially relative to the second clamping part, the carrier rod and the sleeve can be connected and the biological tissue in the carrier rod can be transferred to the sleeve, and the operation of transferring biological tissue such as an embryo is simple and the success rate is high. BRIEF DESCRIPTION OF DRAWINGS

[0017] Fig. 1 is a schematic view of a clamp according to an embodiment of the utility model;

[0018] Fig. 2 is a schematic view of a first clamping part according to an embodiment of the utility model;

[0019] Fig. 3 is a schematic view of a carrier rod according to an embodiment of the utility model;

[0020] Fig. 4 is a schematic view of a clamp according to another embodiment of the utility model;

[0021] Figs. 5A and 5B are schematic views of a connecting part according to an embodiment of the utility model;

[0022] Fig. 6 is a schematic view of a clamp according to still another embodiment of the utility model;

[0023] Fig. 7 is a schematic view of a carrier bar support frame carrying a carrier bar according to an embodiment of the present application. DETAILED DESCRIPTION

[0024] In order to make the objectives, technical solutions and advantages of the present application clearer, the technical solutions of the present application will be described below in detail with reference to the embodiments of the present application and the corresponding drawings. Obviously, the described embodiments are only some of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0025] The technical solutions provided by the embodiments of the present application will be described in detail below with reference to the drawings.

[0026] Referring to Fig. 1, a clamp 1 for biological tissue transfer according to an embodiment of the present application comprises a clamp body 10, a first clamping portion 11 and a second clamping portion 12. The first clamping portion 11 and the second clamping portion 12 are oppositely arranged on the clamp body 10, wherein the first clamping portion 11 is used to clamp and fix a carrier bar 20, and the second clamping portion 12 is used to clamp and fix a sleeve 30.

[0027] With reference to Figs. 1 and 2, the first clamping portion 11 is installed close to one end of the clamp body 10. The first clamping portion 11 is used to clamp the carrier bar 20 and can drive the carrier bar 20 to move axially relative to the clamp body 10. Specifically, the first clamping portion 11 is defined with one or more clamping grooves 111, which can also be referred to as carrier bar clamping grooves. The carrier bar 20 can be clamped in the clamping grooves 111 and locked in the clamping grooves 111 by a locking structure 112, wherein the locking structure 112 can be a pin shaft. With continued reference to the drawings, a sliding block 113 is fixedly connected below the first clamping portion 11. The sliding block 113 can slide axially on the clamp body 10, so that the sliding block 113 can drive the first clamping portion 11 and the carrier bar 20 clamped thereby to reciprocate axially on the clamp body 10.

[0028] Referring to Fig. 3, the carrier bar 20 is generally in the shape of a long strip, comprising a handle 21, a carrier bar body 22 and a front end sheet 23. The handle 21 is a position directly contacted and operated by an operator's hand. The front end sheet 23 has at least one groove 231, for example, two or more grooves can be provided. The groove 231 is used to hold biological materials or biological tissues such as embryos, ova or cells and related solutions for protecting these biological materials or biological tissues. When the carrier bar 20 is fixed, the position close to the handle 21 is clamped in the clamping groove 111.

[0029] Continuing to refer to FIG. 1, a second clamping portion 12 for clamping and fixing the sleeve 30 is arranged at the other end of the clamp body 10. The second clamping portion 12 comprises one or more clamping grooves, which can also be referred to as sleeve clamping grooves. The height of the clamping grooves of the second clamping portion 12 is the same as that of the carrier rod clamping grooves of the first clamping portion 11, so that the sleeve 30 and the carrier rod 20 are in the same horizontal position and the end portions are opposite to each other, facilitating the butt joint or contact of the sleeve 30 and the carrier rod 20. The sleeve 30 has a volume of 0.25-0.5 mL, which can be one of a straw, a centrifuge tube, and a test tube, is made of a biocompatible material, and can be used for cryopreservation of oocyte and embryo samples or for transferring embryos or oocytes into target animals. In the embodiment, the second clamping portion 12 is fixedly connected with the clamp body 10 or is integrally formed with the clamp body 10. When the carrier rod 20 (clamped by the first clamping portion 11) moves axially relative to the clamp body 10, the sleeve 30 (clamped by the second clamping portion 12) is fixed relative to the clamp body 10. It should be noted that the axial movement can be reciprocating movement, shaking, rocking, or movement, and the carrier rod 20 and the sleeve 30 always remain in contact during the above-mentioned movement.

[0030] With reference to FIGS. 4, 5A, and 5B, in some embodiments of the utility model, in order to facilitate the butt joint of the carrier rod 20 and the sleeve 30 and accurately align them, a butt joint portion 13 can also be arranged between the first clamping portion 11 and the second clamping portion 12. The butt joint portion 13 is a detachable component for disassembly, cleaning, and disinfection. It comprises a horn-shaped portion that defines the butt joint position of the carrier rod 20 and the sleeve 30. The carrier rod 20 and the sleeve 30 approach the butt joint portion 13 from both sides of the butt joint portion 13. The horn-shaped portion of the butt joint portion 13 is open at both ends and expands outward from the inside, which receives and guides the carrier rod 20 and the sleeve 30, facilitating the alignment of the end portions of the carrier rod 20 and the sleeve 30. Specifically, at least one carrier rod positioning hole 131 and at least one sleeve positioning hole 132 are respectively arranged on both sides of the butt joint portion 13. The carrier rod positioning hole 131 is arranged towards the first clamping portion 11, and the sleeve positioning hole 132 is arranged towards the second clamping portion 12. The carrier rod positioning hole 131 and the sleeve positioning hole 132 gradually expand outward from the inside in a horn shape. Correspondingly, the carrier rod positioning hole 131 and the sleeve positioning hole 132 communicate with each other to form a positioning through hole that penetrates through the butt joint portion 13, so as to facilitate the butt joint of the carrier rod 20 and the sleeve 30, i.e., the carrier rod 20 passes through the carrier rod positioning hole 131 to contact or insert into the sleeve 30 positioned through the sleeve positioning hole 132. In order to facilitate the observation of the butt joint of the carrier rod 20 and the sleeve 30, an opening can also be arranged above the carrier rod positioning hole 131.

[0031] In combination with reference to FIGS. 6 and 7, in some embodiments of the present application, the clamp 1 can further comprise one or more carrier rod support frames 40. The carrier rod support frame 40 has a groove body capable of simultaneously carrying one or more carrier rods. The carrier rod support frame 40 is arranged on the clamp body 10 and can be mounted between the first clamping portion 11 and the butt joint portion 13 or between the first clamping portion 11 and the second clamping portion 12. The carrier rod support frame 40 can allow multiple carrier rods to be clamped and fixed by the first clamping portion 11, and can allow multiple carrier rods to be moved together more conveniently, thereby improving work efficiency. It should be noted that the biological tissue transfer device according to the embodiments of the present application can comprise one or more carrier rod support frames 40, and each carrier rod support frame 40 can be used to carry multiple carrier rods, for example, 1, 2, or 3 carrier rods.

[0032] In the embodiments of the present application, the first clamping portion 11 can move axially by electromagnetic force, mechanical force, friction force, etc., to detach the biological tissue such as embryos carried in the groove of the carrier rod 20 into the sleeve 30. Specifically, the clamp 1 can further comprise a driving device and a reset device (not shown in the figure) for realizing the axial reciprocating motion of the first clamping portion 11. The driving device can accelerate the movement of the sliding block 113, thereby driving the first clamping portion 11 to move in a direction away from the second clamping portion 12. In practice, the driving device can be selected as an electromagnet. The reset device is used to generate a reset action. In practice, the reset device can be selected as a motor and a spring to realize the automatic reset function. In this way, through the cooperation of the driving device and the reset device, the sliding block 113 can perform axial reciprocating motion, thereby driving the carrier rod 20 clamped by the first clamping portion 11 to also perform axial reciprocating motion. The number of reciprocating motions can be 1 to 100 times, or 1-10 times. In addition, the front end stroke of the carrier rod is 0.01mm-30mm, or 0.01mm-5.5mm. Further, in some embodiments of the present application, the clamp can further comprise a sensor (not shown in the figure) for detecting the axial movement of the sliding block 113 relative to the clamp body 10. Through the sensor, it can be determined whether the sliding block 113 has completed one axial movement and reset to the original position.

[0033] According to the embodiments of the present application, by arranging the first clamping portion and the second clamping portion opposite to each other, the first clamping portion clamping the carrier rod carrying the biological tissue can move axially relative to the second clamping portion clamping the sleeve, thereby automatically transferring the biological tissue in the carrier rod into the sleeve. The transfer operation of biological tissue such as embryos by the clamp is simple and has a high success rate, and is particularly suitable for application in a pasture environment (for example, cattle). Further, by arranging the butt joint portion between the first clamping portion and the second clamping portion, the carrier rod and the sleeve can be accurately aligned, thereby further improving the success rate of biological tissue transfer.

[0034] While the present disclosure has been described in detail with respect to particular embodiments, it will be understood that various changes and modifications can be made therein without departing from the spirit and scope of the embodiments. Accordingly, it is intended that the present application cover all such modifications and changes as fall within the scope of the application.

Claims

1. A clamp for biological tissue transfer, characterized by, The application relates to a biological tissue transfer device, comprising: a first clamping part provided with at least one carrier rod clamping groove in which a carrier rod is clamped, and the end of the carrier rod is loaded with biological tissue; a second clamping part provided with at least one sleeve clamping groove in which a sleeve is clamped; wherein the end of the carrier rod and the end of the sleeve are opposite to each other, and the first clamping part moves in the axial direction to transfer the biological tissue loaded in the carrier rod into the sleeve.

2. The clamp of claim 1, wherein The application further comprises: a docking part which is detachably arranged between the first clamping part and the second clamping part, and the docking part is provided with at least one positioning through hole through which the carrier rod passes to make the end of the carrier rod and the end of the sleeve opposite to each other.

3. The clamp of claim 2, wherein The positioning through hole comprises a carrier rod positioning hole which is arranged towards the first clamping part and a sleeve positioning hole which is arranged towards the second clamping part, and the carrier rod positioning hole and the sleeve positioning hole are communicated, and the carrier rod positioning hole and the sleeve positioning hole are gradually expanded from inside to outside in a trumpet shape.

4. The clamp of claim 3, wherein An opening is arranged above the carrier rod positioning hole.

5. The clamp of claim 1, wherein The application further comprises: one or more carrier rod supporting frames, and each carrier rod supporting frame carries a plurality of carrier rods.

6. The clamp of claim 1, wherein The first clamping part further comprises a locking structure which locks the carrier rod in the carrier rod clamping groove.

7. The clamp of claim 1, wherein The application further comprises: a sliding block which is arranged below the first clamping part and drives the first clamping part to move in the axial direction.

8. The clamp of claim 1, wherein The height of the sleeve clamping groove and the height of the carrier rod clamping groove are equal.

9. The clamp of claim 1, wherein The carrier rod is provided with a groove near the front end, and the groove is loaded with at least a liquid containing biological tissue.

10. The clamp of claim 1, wherein The biological tissue comprises an embryo or an ovum. ​

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