Composition for alleviating geriatric body odor using lysimachia davurica extract

Abstract

Disclosed in the present invention is a composition for alleviating geriatric body odor using a Lysimachia davurica extract which removes 2-nonenal, which is a substance that causes geriatric body odor, and inhibits the production of 2-nonenal from palmitoleic acid.

Description

Composition for improving senile body odor using millet grass extract

[0001] The present invention relates to a composition for improving senile body odor using an extract of Lysimachia davurica (or Lysimachia vulgaris var. davurica R. Kunth).

[0002] Lipid peroxidation in tissues is the breakdown of polyunsaturated fatty acids, which are membrane components, by reactive oxygen species and is directly related to drug toxicity, liver damage, and aging, and is also associated with the development of many diseases such as atherosclerosis, diabetes, cancer, and rheumatoid arthritis.

[0003] Lipid peroxidation generates various reactive substances, and aldehydes are the major reactive substances produced by this lipid peroxidation, causing modifications to various proteins and disrupting cellular functions. Representative examples of these aldehydes include 2-alkenal, 4-hydroxy-2-alkenal, and 4-oxo-2-alkenal (Uchida K. (2000) Free Radic. Biol. Med. 28, 1685-1696; Marnett LJ, Riggins JN, West JD (2003) J. Clin. Invest. 111, 583-593).

[0004] 2-alkenal is a highly reactive aldehyde containing two electrophilic reaction centers. 2-alkenal primarily reacts with the SH group of cysteine, the ε-amino group of lysine, and the imidazole group of histidine, which are amino acids found in proteins (Esterbauer H., Schaur RJ, Zollner H. (1991) Free Radic. Biol. Med. 11, 81-128; Uchida K. (2000) Free Radic. Biol. Med. 28, 1685-1696).

[0005] Among 2-alkenals, 2-nonenal, an unsaturated aldehyde, is a substance that causes a characteristic unpleasant odor of old age. 2-nonenal is also called 3-hexyl-2-propenal, 3-hexyl acrolein, heptylideneacetaldehyde, β-hexylacrolein, α-nonenyl aldehyde, nonylenic aldehyde, or trans-2-nonenal, and is produced from the oxidative decomposition of unsaturated fatty acids, such as palmitoleic acid (or 9-hexadecenoic acid), by reactive oxygen species. 2-nonenal is produced as the expression or function of enzymes that remove reactive oxygen species, which are the cause of 2-nonenal production, weakens due to aging, and forms age-related body odor. This is supported by reports showing that the amount of 2-nonenal increases in the body odor of people over 40 years of age, based on the analysis of body odor components from subjects' shirts using gas chromatography and mass spectrometry (Esterbauer H., Schaur RJ, Zollner H. (1991) Free Radic. Biol. Med. 11, 81-128; Haze S., Gozu Y., Nakamura S., Kohno Y., Sawano K., Ohta H., Yamazaki K. (2001) J. Invest. Dermatol. 116, 520-524).

[0006] Methods for deodorizing 2-nonenal have been proposed, such as inhibiting the oxidation of palmitoleic acid to inhibit the formation of 2-nonenal, removing the 2-nonenal component itself, or utilizing a masking effect by fragrances.

[0007] The present invention discloses the inhibitory effect of 2-nonenal production and the removal effect of the 2-nonenal component itself by millet grass extract.

[0008] The objective of the present invention is to provide a composition for improving senile body odor using millet grass extract.

[0009] Other objects or specific objects of the present invention will be presented below.

[0010] The present invention is completed by confirming that, as confirmed in the examples below, the millet extract removes 2-nonenal, a substance causing senile body odor, in a concentration-dependent manner, and also inhibits the production of 2-nonenal from palmitoleic acid in a concentration-dependent manner.

[0011] Considering the above, the present invention can be understood as a composition for improving senile body odor or 2-nonenal, comprising millet grass extract as an active ingredient.

[0012] In this specification, "millet extract" refers to an extract obtained by leaching the stem, leaf, seed, root, flower, etc. of millet to be extracted using water, a lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.), methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixture thereof, an extract obtained using a supercritical extraction solvent such as carbon dioxide or pentane, or a fraction obtained by fractionating the extract. The extraction method may be any method such as cold maceration, reflux, heating, ultrasonic radiation, or supercritical extraction, taking into account the polarity of the active substance, the degree of extraction, and the degree of preservation. The term "fractionated extract" includes fractions obtained by suspending an extract in a specific solvent and then mixing and settling it with a solvent of different polarity, as well as fractions obtained by adsorbing the crude extract onto a column packed with silica gel or the like and using a hydrophobic solvent, a hydrophilic solvent, or a mixture thereof as the mobile phase. Furthermore, the term "extract" includes concentrated liquid extracts or solid extracts from which the extraction solvent has been removed by methods such as freeze-drying, vacuum drying, hot air drying, or spray drying. Preferably, it refers to an extract obtained using water (including hot water), ethanol, or a mixture thereof (containing 10 to 50% ethanol) as the extraction solvent.

[0013] In addition, the term "active ingredient" in this specification means an ingredient that exhibits the intended activity alone or can exhibit activity in combination with an additive that is inactive itself.

[0014] The composition of the present invention may include the active ingredient in any amount (effective amount) as long as it can exhibit the intended activity of removing or suppressing senile body odor, depending on the product, use, formulation, etc., and the typical effective amount will be determined within the range of 0.001 weight % to 99.9 weight % based on the total weight of the composition. Here, "effective amount" refers to the amount of the active ingredient included in the composition of the present invention that can exhibit the intended effect, such as the effect of removing or suppressing senile body odor, when the composition of the present invention is used on a person to whom it is applied. Such an effective amount may be determined experimentally within the ordinary capacity of a person skilled in the art.

[0015] In addition to the active ingredient, the composition of the present invention may further include any compound or natural extract known in the art to have the said activity and whose safety has already been verified, in order to enhance convenience of use through the addition of similar activities such as skin whitening improvement, skin wrinkle improvement, skin moisturization improvement, skin barrier improvement, skin itching improvement activity, hair loss improvement activity, and skin protection activity (such as inhibition of skin damage caused by ultraviolet rays).

[0016] These compounds or extracts include compounds or extracts listed in pharmacopoeias of various countries (in Korea, the "Korean Pharmacopoeia," "Standards and Test Methods for Quasi-drugs," etc.), functional cosmetics of various countries (in Korea, the "Standards and Test Methods for Functional Cosmetics" notified by the Ministry of Food and Drug Safety), compounds or extracts that have received product approval in accordance with the laws of various countries governing the manufacture and sale of pharmaceuticals (in Korea, the "Pharmaceutical Affairs Act"), and compounds or extracts whose functionality has been recognized in accordance with the laws of various countries governing the manufacture and sale of functional cosmetics (in Korea, the "Cosmetics Act").

[0017] Examples of such ingredients include arbutin, niacinamide, ascorbyl glucoside, alpha-bisabolol, and oil-soluble licorice (Glycyrrhiza) extract, which are recognized as skin whitening ingredients in the Cosmetic Code of Korea under the Korean Cosmetic Act (Notification of the Ministry of Food and Drug Safety, “Standards and Test Methods for Functional Cosmetics”); retinol, retinyl palmitate, adenosine, and polyethoxylated amide, which are recognized as skin wrinkle improvement ingredients in the same Korean Cosmetic Code; drometrizole, drometrizole trisiloxane, digalloyl trioleate, dimethicodiethylbenzalmalonate, and diethylhexyl butamidotriazone, which are recognized as UV protection ingredients in the same Korean Cosmetic Code; and dexpanthenol, biotin, L-menthol, and zinc pyrithione, which are recognized as hair loss symptom improvement ingredients in the same Korean Cosmetic Code.

[0018] The composition of the present invention may be understood as a topical skin composition in terms of its mode of use. If understood as a topical skin composition, the topical skin composition may take on any product classification in terms of legal, intended use, or formulation. Specifically, in terms of legal product classification, it may take on any product classification, such as non-functional general cosmetics, functional cosmetics, or pharmaceutical compositions such as quasi-drugs. If it is a functional cosmetic, it may be a cosmetic having any intended use, such as whitening function, wrinkle improvement function, skin moisturizing function, skin barrier improvement function, or skin itching improvement function. In terms of formulation, it may be any form such as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, oil, or spray.

[0019] The composition of the present invention can be understood as a cosmetic composition in specific embodiments.

[0020] When the composition of the present invention is identified as a cosmetic composition, the product form may take any product form. Specifically, it may take the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, etc. In terms of specific product forms, it may be formulations such as softening lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, or powder.

[0021] The cosmetic composition of the present invention may include, in addition to the active ingredient, ingredients commonly used in cosmetic compositions, such as stabilizers, solubilizers, surfactants, vitamins, colorants, and fragrances, and a carrier.

[0022] In the case where the formulation of the present invention is a paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.

[0023] In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane / butane, or dimethyl ether.

[0024] When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent, or an emulsifying agent is used as a carrier component, specifically, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, etc. may be used.

[0025] In the case where the formulation of the present invention is a suspension, liquid diluents such as water, ethanol, or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, etc. may be used as carrier components.

[0026] In the case where the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester, etc. may be used as a carrier component.

[0027] The cosmetic composition of the present invention can be prepared according to the method of preparing cosmetic compositions commonly practiced in the art, except that it includes an active ingredient that exhibits activity in removing senile body odor.

[0028] The composition of the present invention can be understood as a soap composition in other specific embodiments.

[0029] When the external skin preparation composition of the present invention is identified as a soap composition, the soap composition of the present invention may be prepared by including the active ingredient in a soap base, and may be prepared by including a skin moisturizer, an emulsifier, a water softener, etc., as an additive.

[0030] As the above soap base, vegetable oils such as palm oil, palm oil, soybean oil, castor oil, olive oil, palm kernel oil, etc., or animal oils such as beef tallow, lard, lamb fat, fish oil, etc., may be used; as the above skin moisturizer, glycerin, erythritol, polyethylene glycol, propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol, etc., may be used; as the above emulsifier, natural oils, waxes, fatty alcohols, hydrocarbons, natural plant extracts, etc., may be used; and as the above water softener, tetrasodium EDTA, etc., may be used.

[0031] The soap composition of the present invention may also additionally include, as additives, antimicrobial agents, dispersants, antifoaming agents, solvents, anti-scale agents, corrosion inhibitors, fragrances, colorants, metal ion chelating agents, antioxidants, preservatives, etc. These additives are known in the art, and any of the known ones may be used.

[0032] In the soap composition of the present invention, the soap base or additive may be included in amounts generally used in the art, and the soap base may generally be added in an amount of 99.9% by weight to 10.0% by weight based on the total content of the soap composition, and the additive may be added in an amount of 0.01% by weight to 20.00% by weight.

[0033] The composition of the present invention may be understood as a pharmaceutical composition in another specific embodiment. If the composition of the present invention is understood as a pharmaceutical composition, the pharmaceutical composition may be legally classified as a pharmaceutical composition or a quasi-pharmaceutical composition. Depending on the country, such as Korea and Japan, articles used for the purpose of diagnosing, treating, improving, alleviating, managing, or preventing diseases in humans or animals that have a milder effect than pharmaceuticals are classified separately as quasi-pharmaceuticals. In the present invention, the term "pharmaceutical composition" implies that, in countries with such legal classifications, such quasi-pharmaceuticals are included in addition to pharmaceuticals.

[0034] The pharmaceutical composition of the present invention may be prepared as an oral or parenteral formulation according to the route of administration by conventional methods known in the art, including a pharmaceutically acceptable carrier in addition to the active ingredient. The route of administration may be any suitable route including a local route, an oral route, an intravenous route, an intramuscular route, and direct absorption through mucosal tissues, and may also be used in combination of two or more routes. An example of a combination of two or more routes is a case where two or more formulations of drugs according to the route of administration are combined, for example, one drug is administered first via the intravenous route and another drug is administered second via the local route.

[0035] Pharmaceutically acceptable carriers are known in the art according to the route of administration or dosage form, and specifically, one may refer to the pharmacopoeias of each country, including the "Korean Pharmacopoeia."

[0036] When the pharmaceutical composition of the present invention is prepared as an oral formulation, it may be prepared in the form of powder, granules, tablets, pills, coated tablets, capsules, liquids, gels, syrups, suspensions, wafers, etc., in accordance with methods known in the art together with a suitable carrier. Examples of suitable carriers include sugars such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol, and xylitol; starches such as corn starch, potato starch, and wheat starch; celluloses such as cellulose, methylcellulose, ethylcellulose, sodium carboxymethylcellulose, and hydroxypropylmethylcellulose; polyvinylpyrrolidone; water; methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate; mineral oil; malt; gelatin; talc; polyols; vegetable oils; ethanol; glycerol, etc. When formulating, appropriate binders, lubricants, disintegrants, coloring agents, diluents, etc. may be included as needed. Suitable binders include starch, magnesium aluminum silicate, starch ferrite, gelatin, methylcellulose, sodium carboxymethylcellulose, polyvinylpyrrolidone, glucose, corn sweeteners, sodium alginate, polyethylene glycol, wax, etc. Suitable lubricants include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, silica, talcum, stearic acid, its magnesium and calcium salts, polyethylene glycol, etc. Suitable disintegrants include starch, methylcellulose, agar, bentonite, xanthan gum, starch, alginic acid, or its sodium salt, etc. In addition, diluents include lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, glycine, etc.

[0037] When the pharmaceutical composition of the present invention is prepared as a parenteral formulation, it may be formulated in the form of an injectable, transdermal, nasal inhalant, and suppository according to methods known in the art with a suitable carrier. When formulated as an injectable, an aqueous isotonic solution or suspension may be used as a suitable carrier; specifically, isotonic solutions such as PBS (phosphate buffered saline) containing triethanolamine, sterile water for injection, or 5% dextrose may be used. When formulated as a transdermal formulation, it may be formulated in the form of an ointment, cream, lotion, gel, topical solution, paste, liniment, aerosol, etc. In the case of nasal inhalers, they can be formulated in the form of an aerosol spray using suitable propellants such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, and carbon dioxide, and when formulated as suppositories, the carriers may include Witepsol, Tween 61, polyethylene glycols, cocoa starch, laurin starch, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearate, and sorbitan fatty acid esters.

[0038] Specific formulations of pharmaceutical compositions are known in the art, and reference may be made to literature such as [Remington's Pharmaceutical Sciences (19th ed., 1995)]. The said literature is to be considered part of this specification.

[0039] A preferred dosage of the pharmaceutical composition of the present invention may be in the range of 0.001 mg / kg to 10 g / kg per day, preferably 0.001 mg / kg to 1 g / kg, depending on the patient's condition, body weight, gender, age, severity of the patient, and route of administration. Administration may be administered once a day or divided into several doses. Such dosages shall not be construed as limiting the scope of the present invention in any aspect.

[0040] The pharmaceutical composition of the present invention may be prepared in topical formulations, such as creams, lotions, ointments (semi-solid external medicines), microemulsions, gels, pastes, etc., by including a pharmaceutically acceptable carrier in addition to the active ingredient.

[0041] The composition of the present invention can be understood as a food composition in specific embodiments.

[0042] The food composition of the present invention can be manufactured in any form, for example, as beverages such as tea, juice, carbonated drinks, and isotonic drinks; processed dairy products such as milk and yogurt; chewing gums; rice cakes, Korean confectionery, bread, cookies, and noodles; and health functional food preparations such as tablets, capsules, pills, granules, liquids, powders, flakes, pastes, syrups, gels, jellies, and bars.

[0043] In addition, the food composition of the present invention may have any product classification in terms of legal and functional classification, as long as it complies with the regulations in effect at the time of manufacture and distribution. For example, it may be a health functional food under the Korean "Act on Health Functional Foods," or a confectionery, legume, tea, beverage, special dietary food, etc., according to each food type in the Food Code of the Korean "Food Sanitation Act" (Notification of the Ministry of Food and Drug Safety "Standards and Specifications for Food").

[0044] The food composition of the present invention may include food additives in addition to its active ingredients. Food additives can generally be understood as substances added to, mixed with, or permeated into food during the manufacture, processing, or preservation of food; since they are consumed daily and over a long period of time along with food, their safety must be guaranteed. Food additive codes in accordance with the laws of each country governing the manufacture and distribution of food (the "Food Sanitation Act" in Korea) restrictively define food additives with guaranteed safety in terms of composition or function. In the Korean Food Additive Code (Notification of the Ministry of Food and Drug Safety, "Standards and Specifications for Food Additives"), food additives are classified and defined in terms of composition into chemically synthesized products, natural additives, and mixed preparations; and in terms of function, these food additives are classified into sweeteners, flavoring agents, preservatives, emulsifiers, acidulants, thickeners, etc.

[0045] Sweeteners are used to impart a suitable sweetness to food, and both natural and synthetic sweeteners can be used in the composition of the present invention. Preferably, natural sweeteners are used, and examples of natural sweeteners include corn syrup solids, honey, sucrose, fructose, lactose, maltose, etc.

[0046] Flavoring agents can be used to improve taste or aroma, and both natural and synthetic ones may be used. Preferably, natural ones are used. When natural ones are used, nutritional enhancement can be achieved in addition to flavor. Natural flavoring agents may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, etc., or from green tea leaves, Solomon's seal, bamboo leaves, cinnamon, chrysanthemum leaves, jasmine, etc. Additionally, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, ginkgo, etc., may be used. Natural flavoring agents may be liquid concentrates or solid extracts. In some cases, synthetic flavoring agents may be used, and synthetic flavoring agents may include esters, alcohols, aldehydes, terpenes, etc.

[0047] Calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), etc. may be used as preservatives, and acacia gum, carboxymethylcellulose, xanthan gum, pectin, etc. may be used as emulsifiers, and acetic acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, etc. may be used as acidifiers. Acidifiers may be added to the food composition to achieve an appropriate acidity, in addition to for the purpose of enhancing flavor, for the purpose of inhibiting the growth of microorganisms.

[0048] As thickening agents, suspending agents, settling agents, gel-forming agents, puffing agents, etc. may be used.

[0049] In addition to the food additives described above, the food composition of the present invention may include physiologically active substances or minerals known in the art and whose stability as food additives is guaranteed, for the purpose of supplementing and reinforcing functionality and nutritional value.

[0050] Examples of such physiologically active substances include catechins contained in green tea, vitamins such as vitamin B1, vitamin C, vitamin E, and vitamin B12, tocopherol, dibenzoylthiamine, etc., and examples of minerals include calcium preparations such as calcium citrate, magnesium preparations such as magnesium stearate, iron preparations such as iron citrate, chromium chloride, potassium iodide, selenium, germanium, vanadium, zinc, etc.

[0051] The food composition of the present invention may include the food additives described above in an appropriate amount to achieve the purpose of their addition, depending on the product type.

[0052] Regarding other food additives that may be included in the food composition of the present invention, reference may be made to the food codes or food additive codes of each country.

[0053] As described above, according to the present invention, a composition for improving senile body odor using millet extract can be provided.

[0054] The composition of the present invention can be commercialized into products such as cosmetics, quasi-drugs, and soaps for the purpose of removing elderly body odor.

[0055] Figure 1 shows the results of removing 2-nonenal, a substance that causes senile body odor, using millet extract.

[0056] Figure 2 shows the results of the inhibitory effect of millet grass extract on the production of 2-nonenal, a substance that causes senile body odor.

[0057] The present invention will be described below with reference to examples. However, the scope of the present invention is not limited to these examples.

[0058]

[0059] <Example> Effect of Millet Extract on Removing Elderly Body Odor

[0060] 1. Preparation of Millet grass extract

[0061] To obtain the millet extract, 1 L of distilled water was added to 50 g of the dried whole plant of millet with moisture removed, and the mixture was heated at 100°C for 3 hours. The extract was filtered, concentrated under reduced pressure, and freeze-dried using a freeze-dryer at -40°C to 40°C for 72 hours to obtain 7.6 g (15.2%) of the millet hot water extract.

[0062] In addition, 1 L of 30% ethanol was added to 50 g of dried millet grass whole plant and heated at 60°C for 3 hours to extract, and the obtained extract was filtered and concentrated under reduced pressure and then freeze-dried to obtain 6.2 g (12.4%) of millet grass 30% ethanol extract.

[0063] 2. Experiment on the effectiveness of removing elderly body odor

[0064] 2.1 Experiment on 2-Nonenal Removal Effect

[0065] To evaluate the deodorizing power against 2-nonenal, a major causative substance of elderly body odor, under physiological conditions, an experiment was conducted as follows by adding hot water and 30% ethanol extracts of *Scutellaria baicalensis*. 0.1 mM 2-nonenal was added to 1 mL of phosphate buffer solution (pH 7.4) containing hot water and 30% ethanol extracts of *Scutellaria baicalensis* at concentrations of 0.5, 1, and 10 mg / mL, and the mixture was stirred for 24 hours in a water bath maintained at 37°C. Afterward, the reaction mixture was filtered, and the deodorizing power against elderly odor was confirmed by evaluating the 2-nonenal removal effect through HPLC analysis according to the following conditions.

[0066] HPLC analysis conditionsSystemWaters HPLC system e2695ColumnYMC Pack pro C18 (4.6mm × 250mm, 5μm)Flow rate1.0mL / minDetectorPDA (226nm)Injection vol.20μLColumn temp.30℃Mobile phaseA: 0.1% Formic acid in AcetonitrileB: 0.1% Formic acid in water0 - 30 min, 10 - 100% A; 40 min, 100% A

[0067] The results are shown in Table 2 and Figure 1. Referring to Table 2 and Figure 1, it was confirmed that when hot water extracts and 30% ethanol extracts of *Scutellaria baicalensis* at concentrations of 0.5, 1, and 10 mg / mL were reacted with 2-nonenal, the deodorizing power was improved, with lower levels of 2-nonenal detected in proportion to the treatment concentration of the extract. The deodorizing effect of 2-nonenal was higher in the 30% ethanol extract than in the hot water extract, and at 10 mg / mL treatment, the hot water extract and 30% ethanol extract showed 2-nonenal removal effects of 51.65% and 76.77%, respectively.

[0068] 2-Nonenal Removal Effect 0.5 mg / mL 1 mg / mL 10 mg / mL Deodorizing Power (%) Standard Deviation Deodorizing Power (%) Standard Deviation Deodorizing Power (%) Standard Deviation Millet flower hot water extract 13.13 1.28 24.18 0.62 51.65 0.38 Millet flower 30% ethanol extract 34.88 2.944 1.23 1.00 76.77 2.74

[0069] 2.2 Experiment on the inhibitory effect of 2-nonenal production

[0070] The effect of adding millet extract to the production stage of 2-nonenal, a major causative substance of senile body odor, by heating fatty acids was investigated.

[0071] 100 mg of palmitoleic acid as a fatty acid, 500 mg of cellulose powder, and 1.25 mL of 0.12 M phosphate buffer solution (pH 6.0) were placed in a 20 mL glass vial for headspace. 1 mL of 1% extract of *Scutellaria baicalensis* was added for the test group, and 1 mL of distilled water was added for the control group. After sealing the vial lid, the vials were heat-treated at 105°C for 1 hour and cooled to room temperature. Subsequently, the concentration of 2-nonenal was measured using a mass spectrometer with a gas chromatograph equipped with a headspace sample injector according to the conditions in Table 3. The volatile aldehyde 2-nonenal was used as the standard sample, and the concentration of 2-nonenal was calculated based on the peak areas of the standard sample, the test group, and the control group.

[0072] Analysis conditionsHeadspaceAgilent 7697AGCAgilent 8890 GCOven temp.80℃Inlet225℃, Split (10:1)Loop temp.90℃ColumnDB-WAX UI (30m×0.25mm, 0.25μm)Transfer line temp.100℃Oven temp.80℃ (5min) → 5℃ / min → 200℃ (5min)Vial equilibration time5minInjection vol.1μLInjection duration0.5minColumn flow1mL / min (He)GC cycle time41minMSAgilent 7010B Triple Quadrupole GC / MSSource temp.230℃MS1 Quad temp.150℃MS2 Quad temp.150℃Scan typeSIM mode (m / z 55, 83, 111)

[0073] The results are shown in Table 4 and Figure 2. Referring to Table 4 and Figure 2, it was confirmed that the production of 2-nonenal was inhibited when treated with the *Scutellaria baicalensis* extract compared to the control group. The concentration of 2-nonenal produced from palmitoleic acid was 6.48 μg / mL in the control group, and when treated with the *Scutellaria baicalensis* hot water extract and 30% ethanol extract, the concentration of 2-nonenal decreased to 4.08 μg / mL and 2.32 μg / mL, respectively, demonstrating a deodorizing effect. The inhibitory effect on the production of 2-nonenal was 39.60% and 68.87%, which was higher in the 30% ethanol extract than in the hot water extract.

[0074] 2-Nonenal Production Inhibitory Effect Sample 2-Nonenal Concentration (μg / mL) Deodorizing Power (%) Control Group 6.48 - Millet Starch Hot Water Extract 4.08 39.60 Millet Starch 30% Ethanol Extract 2.32 68.87

Claims

1. A composition for improving senile body odor comprising millet grass extract as an active ingredient.

2. In Paragraph 1, The above-mentioned millet extract is a composition in which the millet extract is a water, ethanol, or mixed solvent extract thereof.

3. In Paragraph 1 or 2, The above composition is a composition that is a topical skin preparation composition.

4. In Paragraph 1 or 2, The above composition is a composition that is a cosmetic composition or a soap composition.

5. In Paragraph 1 or 2, The above composition is a pharmaceutical composition.

6. In Paragraph 1 or 2, The above composition is a food composition.

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