A GABA-lysine calcium supplement preparation for promoting calcium absorption

Abstract

The present invention discloses a GABA-lysine calcium supplement preparation for promoting calcium absorption, belonging to the technical field of calcium supplements. The preparation comprises a calcium-source modified microsphere component, an absorption-promoting inclusion component, a calcium-directed deposition component, and a synergistic enhancement component. The calcium-source modified microsphere component is formed by compounding algal calcium, dicalcium phosphate, chitosan, and a phosphorylation reagent, serving as the core calcium source. Surface phosphorylation modification enables the formation of hydrophilic microsphere particles. The absorption-promoting inclusion component is composed of GABA, L-lysine, casein phosphopeptide, and carboxymethyl-β-cyclodextrin, which enhances intestinal calcium absorption. Through functional matching between the microsphere component and the inclusion component, the calcium source maintains structural stability while fully interacting with absorption-promoting ingredients, thereby improving calcium absorption efficiency.

Description

[0001] DESCRIPTION

[0002] A GABA-Lysine Calcium Supplement Preparation for Promoting Calcium Absorption

[0003] Technical Field

[0004] The present invention relates to the technical field of calcium supplement preparations, and particularly to a GABA-lysine calcium supplement preparation for promoting calcium absorption.

[0005] Background Art

[0006] Calcium is an indispensable macronutrient element for the human body and participates in many key physiological activities such as bone formation, nerve conduction, and muscle contraction. The human body cannot synthesize calcium on its own and must obtain it through diet or exogenous supplements. There is a definite need for calcium supplementation during physiological stages such as skeletal development in adolescents, nutritional supply during pregnancy, and bone calcium loss in middle-aged and elderly individuals. With the enhancement of public health awareness, calcium supplement preparations have become a research hotspot in the food and pharmaceutical fields, and market demand for calcium supplementation products suitable for all populations and having complete functions continues to increase.

[0007] However, existing calcium supplement preparations lack systematic and targeted component design. No dedicated modified calcium-source core material is provided, and calcium-source raw materials are merely simply compounded without structural modification, making it difficult to form a stable particulate structure. The absorption-promoting ingredients are not subjected to inclusion treatment and therefore cannot form dedicated absorption-promoting functional materials, which easily leads to the loss of active ingredients. There is no dedicated functional component for bone-directed calcium deposition, and only conventional nutritional ingredients are simply added, making it impossible to realize the directed action of calcium. In addition, there is no rational basis for the addition ratio DESCRIPTION of antioxidant ingredients, and the various components of the preparation lack an effective mode of combination, making it difficult to form an integrated structure and preventing the establishment of a synergistic mechanism among the components.

[0008] Summary of the Invention

[0009] The purpose of the present invention is to overcome the deficiencies of the prior art by providing a GABA-lysine calcium supplement preparation for promoting calcium absorption. In the present invention, algal calcium, dicalcium phosphate, chitosan, and a phosphorylation reagent are compounded to form the calcium-source modified microsphere component, which serves as the basic calcium-source core material of the preparation. Surface phosphorylation modification enables this component to form a hydrophilic microsphere-grade particulate structure. Meanwhile, GABA, L-lysine, casein phosphopeptide, and carboxymethyl- P-cyclodextrin are combined to form a composite inclusion compound serving as the core absorption-promoting material for intestinal calcium absorption. Functional matching between the calcium-source modified microsphere component and the absorption-promoting inclusion component allows the calcium source to maintain a stable structure while fully contacting the absorption-promoting ingredients, thereby establishing the basic conditions for intestinal calcium absorption.

[0010] In order to solve the above technical problems, the present invention provides the following technical solution. In one aspect, a GABA-lysine calcium supplement preparation for promoting calcium absorption is provided, and the preparation comprises, by parts by weight: a calcium-source modified microsphere component: 60-80 parts; an absorption-promoting inclusion component: 15-25 parts; a calcium-directed deposition component: 3-8 parts; a synergistic enhancement component: 0.05-0.15 part. DESCRIPTION

[0011] Further, the calcium-source modified microsphere component is a compound of algal calcium, dicalcium phosphate, chitosan, and a phosphorylation reagent, wherein the mass ratio of algal calcium, dicalcium phosphate, chitosan, and the phosphorylation reagent in the calcium-source modified microsphere component is 80:18-22:9-11:2.8-3.2, and the calcium-source modified microsphere component is a hydrophilic composite calcium-source microsphere prepared by surface phosphorylation modification and having a microsphere-grade particulate structure, serving as the basic calcium-source core material of the preparation.

[0012] Further, the absorption-promoting inclusion component is a composite inclusion compound of GABA, L-lysine, casein phosphopeptide, and carboxymethyl-P-cyclodextrin, with an inclusion rate of >95%, and serves as the core absorption-promoting material for efficient intestinal calcium absorption, wherein the molar ratio of GABA to L-lysine is 1:1.5, the addition amount of casein phosphopeptide is 4.8-5.2% of the total mass of GABA and L-lysine, and the mass ratio of the mixture of GABA, L-lysine, and casein phosphopeptide to carboxymethyl-P-cyclodextrin is 0.8-1.2: 1.8-2.2.

[0013] Further, the calcium-directed deposition component is a composition of K2MK7 and natural VD2, in a uniform fat-soluble liquid structure, and serves as a functional material for directed skeletal deposition of calcium, wherein K2MK7 and natural VD2 are extracted from natto and Agaricus bisporus, respectively, and the mass ratio of K2MK7 to natural VD2 is 0.8-1.2:18-22.

[0014] Further, the synergistic enhancement component is natural vitamin E, and the addition amount thereof is 0.08-0.12% of the total mass of the calcium-source modified microsphere component, the absorption-promoting inclusion component, and the calcium-directed deposition component. This synergistic enhancement component serves as an antioxidant protective material for the active ingredients.

[0015] Further, the preparation is prepared from the calcium-source modified DESCRIPTION microsphere component, the absorption-promoting inclusion component, the calcium-directed deposition component, and the synergistic enhancement component to form an integrated composite microsphere structure. Gum arabic binder is additionally added during preparation, and the addition amount of the gum arabic binder is 1.8-2.2% of the total mass of the calcium-directed deposition component and the absorption-promoting inclusion component. The gum arabic binder is used to enhance the adsorption and bonding effect among the components, thereby ensuring the integrity of the integrated composite microsphere structure.

[0016] In another aspect, a method for preparing the GABA-lysine calcium supplement preparation for promoting calcium absorption is provided, comprising: preparing calcium-source microspheres: mixing algal calcium, dicalcium phosphate, chitosan, and a phosphorylation reagent, preparing basic calcium-source microspheres by an emulsification-crosslinking method, and subjecting the basic calcium-source microspheres to surface phosphorylation modification to obtain the calcium-source modified microsphere component; preparing a composite inclusion compound: mixing GABA, L-lysine, casein phosphopeptide, and carboxymethyl-P-cyclodextrin, and forming an inclusion structure by a saturated aqueous solution method to obtain the absorption-promoting inclusion component; preparing a deposition component: extracting K2MK7 from natto and natural VD2 from Agaricus bisporus, mixing K2MK7 with natural VD2 and then purifying the mixture to obtain the calcium-directed deposition component; preparing an inclusion suspension: mixing the calcium-directed deposition component with the absorption-promoting inclusion component, adding gum arabic binder, and continuously stirring to form a uniform, particle-free inclusion suspension; DESCRIPTION preparing composite microspheres: immersing the calcium-source modified microsphere component into the inclusion suspension for adsorption treatment, adding the synergistic enhancement component, stirring and mixing uniformly, and subjecting the mixture to a drying process to obtain the integrated composite microsphere preparation.

[0017] Further, in the preparation of the calcium-source microspheres, algal calcium, dicalcium phosphate, chitosan, and the phosphorylation reagent are mixed and then added to deionized water to prepare a uniform suspension, and the basic calcium-source microspheres having a particle size of 50-80 pm are formed in an emulsification apparatus by an emulsification-crosslinking method. The surface phosphorylation modification treatment comprises placing the formed basic calcium-source microspheres in a phosphorylation reagent system for directional surface modification, continuously stirring at 300-500 r / min for 1-2 h during modification, and after completion, washing with deionized water and drying at low temperature to obtain the hydrophilic calcium-source modified microsphere component.

[0018] Further, in the preparation of the composite inclusion compound, carboxymethyl-P-cyclodextrin is added to deionized water to prepare a saturated aqueous solution, and GABA, L-lysine, and casein phosphopeptide are then added. The saturated aqueous solution method is employed in a constant-temperature environment of 40-50°C with continuous stirring at 200-400 r / min for 2-3 h, followed by suction filtration and drying to obtain the absorption-promoting inclusion component having an inclusion rate of >95%.

[0019] Further, in the preparation of the deposition component, natto and Agaricus bisporus are separately pulverized, and K2MK7 and natural VD2 are separately extracted by a supercritical CO2 extraction method under an extraction pressure of 30-40 MPa, an extraction temperature of 45-55°C, and an extraction time of 2-3 h. K2MK7 and natural VD2 are then mixed and DESCRIPTION subjected to chromatographic purification to obtain the calcium-directed deposition component having a purity of >98%.

[0020] Further, in the preparation of the inclusion suspension, the calcium-directed deposition component is mixed with the absorption-promoting inclusion component, gum arabic binder is added, and the mixture is continuously stirred at 400-600 r / min until the system becomes uniform and free of solid particles, thereby obtaining the inclusion suspension.

[0021] Further, in the preparation of the composite microspheres, an ultrasonic adsorption method is employed for the adsorption treatment, wherein the calcium-source modified microspheres are immersed in the inclusion suspension and adsorbed for 30 min under an ultrasonic power of 200-300 W. After the synergistic enhancement component is added and mixed uniformly, spray drying is carried out under an inlet air temperature of 160-170°C and an outlet air temperature of 70-80 °C to obtain the integrated composite microsphere preparation having a uniform particle size.

[0022] Compared with the prior art, the GABA-lysine calcium supplement preparation for promoting calcium absorption of the present invention has the following beneficial effects:

[0023] I. In the present invention, algal calcium, dicalcium phosphate, chitosan, and a phosphorylation reagent are compounded to form the calcium-source modified microsphere component, which serves as the basic calcium-source core material of the preparation. Surface phosphorylation modification enables this component to form a hydrophilic microsphere-grade particulate structure. Meanwhile, GABA, L-lysine, casein phosphopeptide, and carboxymethyl-P-cyclodextrin are combined to form a composite inclusion compound serving as the core absorption-promoting material for intestinal calcium absorption. Functional DESCRIPTION matching between the calcium- source modified microsphere component and the absorption-promoting inclusion component allows the calcium source to maintain a stable structure while fully contacting the absorption-promoting ingredients, thereby establishing the basic conditions for intestinal calcium absorption.

[0024] II. In the present invention, effective ingredients extracted from natto and Agaricus bisporus are used to form the calcium-directed deposition component, which serves as a functional material for directed skeletal deposition of calcium. Natural vitamin E is used as the synergistic enhancement component to provide antioxidant protection for various active ingredients in the preparation. Gum arabic binder is additionally added as an auxiliary ingredient, so that the calcium-source modified microsphere component, the absorption-promoting inclusion component, the calcium-directed deposition component, and the synergistic enhancement component are closely combined to form a stable integrated composite microsphere structure. By virtue of this structure, the various functional components realize physical adsorption and molecular bonding, ensuring the orderly exertion of their functions and achieving full-process functional synergy of calcium from absorption to deposition.

[0025] Other advantages, objectives, and features of the present invention will be set forth in part in the following specification and in part will be apparent to those skilled in the art upon examination of the following description, or may be learned from the practice of the present invention.

[0026] Brief Description of the Drawings

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the DESCRIPTION present invention, and those of ordinary skill in the art can also obtain other drawings according to these drawings without creative efforts.

[0028] FIG. 1 is a flow chart of the preparation method of the GABA-lysine calcium supplement preparation for promoting calcium absorption;

[0029] FIG. 2 is a framework diagram of the preparation method of the GABA-lysine calcium supplement preparation for promoting calcium absorption;

[0030] FIG. 3 is a flow chart of preparing the calcium-source microspheres in the preparation method of the GABA-lysine calcium supplement preparation for promoting calcium absorption.

[0031] Detailed Description of the Invention

[0032] In order to further illustrate the technical means adopted by the present invention for achieving the intended invention objectives and the effects thereof, the specific embodiments, structures, features, and effects of the present invention are described in detail below with reference to the drawings and preferred embodiments.

[0033] Example 1:

[0034] Raw material preparation (in parts by weight): calcium-source modified microsphere component: 79.9 parts; absorption-promoting inclusion component: 15 parts; calcium-directed deposition component: 5 parts; synergistic enhancement component: 0.1 part.

[0035] Raw material specifications:

[0036] Calcium-source modified microsphere component: algal calcium, dicalcium phosphate, chitosan, and a phosphorylation reagent, all of which are conventional industrial edible-grade raw materials with a purity of >98%; algal calcium, dicalcium phosphate, chitosan, and the phosphorylation reagent are compounded in a mass ratio of 80:20:10:3; DESCRIPTION

[0037] Absorption-promoting inclusion component: y-aminobutyric acid (GABA), L-lysine, casein phosphopeptide (CPP), and carboxymethyl-P-cyclodextrin, all of which are food-additive-grade raw materials with active ingredient contents of >99%; wherein the molar ratio of GABA to L-lysine is 1:1.5, the amount of casein phosphopeptide is 5% of the total mass of GABA and L-lysine, and the mass ratio of the mixture of GABA, L-lysine, and casein phosphopeptide to carboxymethyl-P-cyclodextrin is 1:2;

[0038] Calcium-directed deposition component: natto and Agaricus bisporus, which are natural edible raw materials, from which K2MK7 and natural VD2 are respectively extracted, in a mass ratio of 1:20;

[0039] Synergistic enhancement component: natural vitamin E with a purity of >98%, added in an amount of 0.1% of the total mass of the calcium-source modified microsphere component, the absorption-promoting inclusion component, and the calcium-directed deposition component;

[0040] Auxiliary raw material: gum arabic binder, used as a binding auxiliary in preparation of the preparation, added in an amount of 2% of the total mass of the calcium-directed deposition component and the absorption-promoting inclusion component.

[0041] Preparation method:

[0042] Algal calcium, dicalcium phosphate, chitosan, and the phosphorylation reagent are mixed in a mass ratio of 80:20: 10:3, added to deionized water to prepare a uniform suspension, and subjected to microsphere formation in an emulsification apparatus by an emulsification-crosslinking method to obtain the basic calcium-source microspheres. The formed basic calcium-source microspheres are then placed in a phosphorylation reagent system for directional surface modification, continuously stirred at 400 r / min for 1.5 h during modification, and after completion, washed with deionized water and dried at low temperature to obtain the hydrophilic calcium-source modified microsphere component, as shown in FIG. 1. DESCRIPTION

[0043] Carboxymethyl-P-cyclodextrin is added to deionized water to prepare a saturated aqueous solution, and then GABA and L-lysine mixed at a molar ratio of 1:1.5 are added, together with casein phosphopeptide in an amount of 5% of the total mass of GABA and L-lysine. The mass ratio of the mixture of GABA, L-lysine, and casein phosphopeptide to carboxymethyl-P-cyclodextrin is 1:2. The saturated aqueous solution method is employed in a constant-temperature environment of 45°C with continuous stirring at 300 r / min for 2.5 h, followed by suction filtration and drying to obtain the absorption-promoting inclusion component, as shown in FIG. 2.

[0044] Natto and Agaricus bisporus are separately pulverized, and K2MK7 and natural VD2 are separately extracted by a supercritical CO2 extraction method under an extraction pressure of 35 MPa, an extraction temperature of 50°C, and an extraction time of 2.5 h. The extracted K2MK7 and natural VD2 are mixed in a mass ratio of 1:20 and chromatographically purified to obtain the calcium-directed deposition component.

[0045] The calcium-directed deposition component is mixed with the absorption-promoting inclusion component, and gum arabic binder in an amount of 2% of the total mass of the two components is added. The mixture is continuously stirred at 500 r / min until the system becomes uniform and free of solid particles, thereby obtaining the inclusion suspension.

[0046] The calcium-source modified microsphere component is immersed in the inclusion suspension and subjected to adsorption for 30 min by an ultrasonic adsorption method under an ultrasonic power of 250 W. Natural vitamin E is then added as the synergistic enhancement component. After sufficient stirring and uniform mixing, spray drying is carried out under an inlet air temperature of 165 °C and an outlet air temperature of 75 °C to obtain the integrated composite microsphere preparation.

[0047] Test results:

[0048] Multiple indicators of the prepared integrated composite microsphere io DESCRIPTION preparation were tested:

[0049] Particle size and uniformity: the overall microsphere particle size of the preparation is 60-90 pm; the microspheres are regular in morphology and uniform in particle size, with no adhesion or breakage;

[0050] Structural integrity: after treatment in a simulated gastric acid environment for 2 h, the integrated composite microsphere preparation shows no structural damage and no component shedding. After transfer to simulated intestinal fluid, it can slowly swell and realize the orderly release of components while maintaining a stable structure;

[0051] Component retention rate: the retention rates of the core active ingredients GABA, L-lysine, K2MK7, and natural VD2 are all >98.5%, and the preparation process does not cause obvious damage to the active ingredients;

[0052] Dispersibility in intestinal fluid: in simulated intestinal fluid, the preparation can be completely dispersed within 3 min, with no calcium-source aggregation, and the contact area between the microspheres and the intestinal fluid meets the requirement;

[0053] Component binding property: after high-speed centrifugation at 8000 r / min for 10 min, no stratification or precipitation occurs. The calcium-source modified microspheres, the absorption-promoting inclusion component, and the calcium-directed deposition component are tightly bound in the system, and the integrated structure is stable;

[0054] Preliminary stability at room temperature: after sealed storage at room temperature for 30 d, the contents of the core active ingredients show no significant decrease, the retention rates are all >97%, and no oxidative deterioration occurs.

[0055] Example 2:

[0056] Raw material preparation (in parts by weight): calcium-source modified microsphere component: 74.9 parts; i i DESCRIPTION absorption-promoting inclusion component: 20 parts; calcium-directed deposition component: 5 parts; synergistic enhancement component: 0.1 part.

[0057] Raw material specifications:

[0058] Calcium-source modified microsphere component: algal calcium, dicalcium phosphate, chitosan, and a phosphorylation reagent, all of which are conventional industrial edible-grade raw materials with a purity of >98%; algal calcium, dicalcium phosphate, chitosan, and the phosphorylation reagent are compounded in a mass ratio of 80:20:10:3;

[0059] Absorption-promoting inclusion component: y-aminobutyric acid (GABA), L-lysine, casein phosphopeptide (CPP), and carboxymethyl-P-cyclodextrin, all of which are food-additive-grade raw materials with active ingredient contents of >99%; wherein the molar ratio of GABA to L-lysine is 1:1.5, the amount of casein phosphopeptide is 5% of the total mass of GABA and L-lysine, and the mass ratio of the mixture of GABA, L-lysine, and casein phosphopeptide to carboxymethyl-P-cyclodextrin is 1:2;

[0060] Calcium-directed deposition component: natto and Agaricus bisporus, which are natural edible raw materials, from which K2MK7 and natural VD2 are respectively extracted, in a mass ratio of 1:20;

[0061] Synergistic enhancement component: natural vitamin E with a purity of >98%, added in an amount of 0.1% of the total mass of the calcium-source modified microsphere component, the absorption-promoting inclusion component, and the calcium-directed deposition component;

[0062] Auxiliary raw material: gum arabic binder, used as a binding auxiliary in preparation of the preparation, added in an amount of 2% of the total mass of the calcium-directed deposition component and the absorption-promoting inclusion component.

[0063] Preparation method:

[0064] Algal calcium, dicalcium phosphate, chitosan, and the phosphorylation DESCRIPTION reagent are mixed in a mass ratio of 80:20: 10:3, added to deionized water to prepare a uniform suspension, and subjected to microsphere formation in an emulsification apparatus by an emulsification-crosslinking method to obtain the basic calcium-source microspheres. The formed basic calcium-source microspheres are then placed in a phosphorylation reagent system for directional surface modification, continuously stirred at 400 r / min for 1.5 h during modification, and after completion, washed with deionized water and dried at low temperature to obtain the hydrophilic calcium-source modified microsphere component, as shown in FIG. 3.

[0065] Carboxymethyl-P-cyclodextrin is added to deionized water to prepare a saturated aqueous solution, and then GABA and L-lysine mixed at a molar ratio of 1:1.5 are added, together with casein phosphopeptide in an amount of 5% of the total mass of GABA and L-lysine. The mass ratio of the mixture of GABA, L-lysine, and casein phosphopeptide to carboxymethyl-P-cyclodextrin is 1:2. The saturated aqueous solution method is employed in a constant-temperature environment of 45°C with continuous stirring at 300 r / min for 2.5 h, followed by suction filtration and drying to obtain the absorption-promoting inclusion component.

[0066] Natto and Agaricus bisporus are separately pulverized, and K2MK7 and natural VD2 are separately extracted by a supercritical CO2 extraction method under an extraction pressure of 35 MPa, an extraction temperature of 50°C, and an extraction time of 2.5 h. The extracted K2MK7 and natural VD2 are mixed in a mass ratio of 1:20 and chromatographically purified to obtain the calcium-directed deposition component.

[0067] The calcium-directed deposition component is mixed with the absorption-promoting inclusion component, and gum arabic binder in an amount of 2% of the total mass of the two components is added. The mixture is continuously stirred at 500 r / min until the system becomes uniform and free of solid particles, thereby obtaining the inclusion suspension. DESCRIPTION

[0068] The calcium-source modified microsphere component is immersed in the inclusion suspension and subjected to adsorption for 30 min by an ultrasonic adsorption method under an ultrasonic power of 250 W. Natural vitamin E is then added as the synergistic enhancement component. After sufficient stirring and uniform mixing, spray drying is carried out under an inlet air temperature of 165 °C and an outlet air temperature of 75 °C to obtain the integrated composite microsphere preparation.

[0069] Preparation evaluation:

[0070] Multiple indicators of the prepared integrated composite microsphere preparation were tested:

[0071] Particle size and uniformity: the overall microsphere particle size of the preparation is 55-85 pm; the microspheres are regular and rounded in morphology and uniform in particle size, with no adhesion or breakage;

[0072] Structural integrity: after treatment in a simulated gastric acid environment for 2 h, the integrated composite microsphere preparation shows no structural damage and no component dissolution or shedding. After transfer to simulated intestinal fluid, the swelling rate is moderate, making it possible to realize the synchronous and orderly release of the absorption-promoting component and the calcium source;

[0073] Component retention rate: the retention rate of absorption-promoting core active ingredients such as GABA and L-lysine is >99.0%, and the retention rates of K2MK7 and natural VD2 are >98.8%. The preparation process does not cause damage to the various active ingredients;

[0074] Dispersibility in intestinal fluid: in simulated intestinal fluid, the preparation can be completely and uniformly dispersed within 2 min, with no calcium-source aggregation or caking, thereby maximizing the contact area between the microspheres and the intestinal fluid;

[0075] Component binding property: after high-speed centrifugation at 8000 r / min for 10 min, no stratification, precipitation, or component dissociation DESCRIPTION occurs. The various functional components are tightly combined with the calcium-source microspheres, and the stability of the integrated structure is excellent;

[0076] Preliminary stability at room temperature: after sealed storage at room temperature for 30 d, the contents of the core active ingredients show no significant fluctuation, the retention rates are all >97.5%, and no oxidative deterioration occurs.

[0077] Comparative Example:

[0078] Raw material preparation (in parts by weight): conventional calcium-source component: 79.9 parts; conventional absorption-promoting component: 15 parts; calcium-directed deposition component: 5 parts; synergistic enhancement component: 0.1 part.

[0079] Raw material specifications:

[0080] Conventional calcium-source component: calcium carbonate and dicalcium phosphate, both being conventional industrial edible-grade calcium-source raw materials with a purity of >98%; calcium carbonate and dicalcium phosphate are directly mixed in a mass ratio of 7:3, without using algal calcium, without chitosan or a phosphorylation reagent, and without any microsphere formation or modification treatment;

[0081] Conventional absorption-promoting component: casein phosphopeptide and vitamin C, both being food-additive-grade raw materials with active ingredient contents of >99%, directly compounded in a mass ratio of 4:1, without using GABA or L-lysine, and without inclusion treatment with carboxymethyl-P-cyclodextrin;

[0082] Calcium-directed deposition component: identical to that in Example 1, namely natto and Agaricus bisporus, which are natural edible raw materials, from which K2MK7 and natural VD2 are respectively extracted, in a mass ratio of 1:20; DESCRIPTION

[0083] Synergistic enhancement component: identical to that in Example 1, namely natural vitamin E with a purity of >98%, added in an amount of 0.1% of the total mass of the conventional calcium-source component, the conventional absorption-promoting component, and the calcium-directed deposition component;

[0084] Auxiliary raw material: xanthan gum, added in an amount of 2% of the total mass of the calcium-directed deposition component and the conventional absorption-promoting component.

[0085] Preparation method:

[0086] Calcium carbonate and dicalcium phosphate are directly mixed in a mass ratio of 7:3, added to deionized water to prepare a suspension, and dried at low temperature to obtain the conventional calcium-source component.

[0087] Casein phosphopeptide and vitamin C are added to deionized water in a mass ratio of 4:1, stirred at 300 r / min for 30 min at room temperature, and directly dried to obtain the conventional absorption-promoting component.

[0088] The preparation method of the calcium-directed deposition component is identical to that in Example 1. Natto and Agaricus bisporus are separately pulverized, and K2MK7 and natural VD2 are separately extracted by a supercritical CO2 extraction method under an extraction pressure of 35 MPa, an extraction temperature of 50°C, and an extraction time of 2.5 h. The extracted K2MK7 and natural VD2 are mixed in a mass ratio of 1:20 and chromatographically purified to obtain the calcium-directed deposition component.

[0089] The calcium-directed deposition component is mixed with the conventional absorption-promoting component, and xanthan gum in an amount of 2% of the total mass of the two components is added. The mixture is continuously stirred at 500 r / min until the system becomes uniform, thereby obtaining a conventional mixed suspension.

[0090] The conventional calcium-source component is added to the conventional DESCRIPTION mixed suspension and stirred uniformly at room temperature. Natural vitamin E is then added as the synergistic enhancement component. After sufficient stirring and uniform mixing, spray drying is carried out under an inlet air temperature of 165 °C and an outlet air temperature of 75 °C to obtain a conventional compounded calcium supplement preparation.

[0091] Test results:

[0092] The conventional compounded calcium supplement preparation obtained was tested for the same indicators as those in Example 1 and Example 2:

[0093] Particle size and uniformity: the product is an irregular granular mixture with a particle size distribution of 30-120 pm and poor particle-size uniformity, with slight adhesion and partial aggregation;

[0094] Structural integrity: after treatment in a simulated gastric acid environment for 2 h, the structural integrity is poor, with slight dissolution and loss of some components. After transfer to simulated intestinal fluid, swelling is irregular and the release rate is excessively fast, without an orderly release effect;

[0095] Component retention rate: the effective active retention rate in the conventional absorption-promoting component is 82.3%, and the retention rates of K2MK7 and natural VD2 are 86.7% due to the lack of effective protective measures. Simulated gastric acid treatment and the preparation process cause a certain degree of damage to the active ingredients;

[0096] Dispersibility in intestinal fluid: in simulated intestinal fluid, complete dispersion cannot be achieved within 8 min, and obvious calcium-source aggregation occurs. The contact area with the intestinal fluid is significantly lower than that of Example 1 and Example 2, and the insufficient contact area affects subsequent absorption efficiency;

[0097] Component binding property: after high-speed centrifugation at 8000 r / min for 10 min, slight stratification and a small amount of precipitation occur. The components are not tightly combined with one another, and the DESCRIPTION structural stability of the system is only average;

[0098] Preliminary stability at room temperature: after sealed storage at room temperature for 30 d, the contents of the core active ingredients decrease significantly, the overall retention rate is 78.5%, and slight oxidative deterioration and slow degradation of some components occur.

[0099] The foregoing merely describes preferred embodiments of the present invention and is not intended to limit the present invention in any form. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art may make certain changes or modifications by using the technical contents disclosed above without departing from the scope of the technical solution of the present invention. Any brief modification, equivalent change, or modification made to the above embodiments according to the technical essence of the present invention, without departing from the content of the technical solution of the present invention, shall still fall within the scope of the technical solution of the present invention.

Claims

CLAIMS1. A GABA-lysine calcium supplement preparation for promoting calcium absorption, characterized in that the preparation comprises, by parts by weight: a calcium-source modified microsphere component: 60-80 parts; an absorption-promoting inclusion component: 15-25 parts; a calcium-directed deposition component: 3-8 parts; a synergistic enhancement component: 0.05-0.15 part.

2. The GABA-lysine calcium supplement preparation for promoting calcium absorption according to claim 1, characterized in that the calcium-source modified microsphere component is a compound of algal calcium, dicalcium phosphate, chitosan, and a phosphorylation reagent, wherein the mass ratio of algal calcium, dicalcium phosphate, chitosan, and the phosphorylation reagent in the calcium-source modified microsphere component is 80:18-22:9-11 :2.8-3.2, and the calcium-source modified microsphere component is a hydrophilic composite calcium-source microsphere prepared by surface phosphorylation modification and having a microsphere-grade particulate structure.

3. The GABA-lysine calcium supplement preparation for promoting calcium absorption according to claim 1, characterized in that the absorption-promoting inclusion component is a composite inclusion compound of GABA, L-lysine, casein phosphopeptide, and carboxymethyl-P-cyclodextrin, with an inclusion rate of >95%, wherein the molar ratio of GABA to L-lysine is 1:1.5, the addition amount of casein phosphopeptide is 4.8-5.2% of the total mass of GABA and L-lysine, and the mass ratio of the mixture of GABA, L-lysine, and casein phosphopeptide to carboxymethyl-P-cyclodextrin is 0.8-1.2:1.8-2.2.

4. The GABA-lysine calcium supplement preparation for promoting calcium absorption according to claim 1, characterized in that the calcium-directed deposition component is a composition of K2MK7 and natural VD2, in a uniform fat-soluble liquid structure, wherein K2MK7 and naturalCLAIMSVD2 are extracted from natto and Agaricus bisporus, respectively, and the mass ratio of K2MK7 to natural VD2 is 0.8-1.2: 18-22.

5. The GAB A-ly sine calcium supplement preparation for promoting calcium absorption according to claim 1, characterized in that the synergistic enhancement component is natural vitamin E, and the addition amount thereof is 0.08-0.12% of the total mass of the calcium-source modified microsphere component, the absorption-promoting inclusion component, and the calcium-directed deposition component.

6. The GAB A-ly sine calcium supplement preparation for promoting calcium absorption according to claim 1, characterized in that the preparation is prepared from the calcium-source modified microsphere component, the absorption-promoting inclusion component, the calcium-directed deposition component, and the synergistic enhancement component to form an integrated composite microsphere structure, and gum arabic binder is additionally added during preparation, the amount of the gum arabic binder being 1.8-2.2% of the total mass of the calcium-directed deposition component and the absorption-promoting inclusion component.

7. The GAB A-ly sine calcium supplement preparation for promoting calcium absorption according to claim 1, characterized in that the preparation method thereof comprises: preparing calcium-source microspheres: mixing algal calcium, dicalcium phosphate, chitosan, and a phosphorylation reagent, preparing basic calcium-source microspheres by an emulsification-crosslinking method, and subjecting the basic calcium-source microspheres to surface phosphorylation modification to obtain the calcium-source modified microsphere component; preparing a composite inclusion compound: mixing GABA, L-lysine, casein phosphopeptide, and carboxymethyl- P-cyclodextrin, and forming an inclusion structure by a saturated aqueous solution method to obtain the absorption-promoting inclusion component;CLAIMS preparing a deposition component: extracting K2MK7 from natto and natural VD2 from Agaricus bisporus, mixing K2MK7 with natural VD2 and then purifying the mixture to obtain the calcium-directed deposition component; preparing an inclusion suspension: mixing the calcium-directed deposition component with the absorption-promoting inclusion component, adding gum arabic binder, and continuously stirring to form a uniform, particle-free inclusion suspension; preparing composite microspheres: immersing the calcium-source modified microsphere component into the inclusion suspension for adsorption treatment, adding the synergistic enhancement component, stirring and mixing uniformly, and subjecting the mixture to a drying process to obtain the integrated composite microsphere preparation.

8. The GAB A-ly sine calcium supplement preparation for promoting calcium absorption according to claim 7, characterized in that in the preparation of the calcium-source microspheres, algal calcium, dicalcium phosphate, chitosan, and the phosphorylation reagent are mixed and then added to deionized water to prepare a uniform suspension, and the basic calcium-source microspheres having a particle size of 50-80 pm are formed in an emulsification apparatus by an emulsification-crosslinking method; the surface phosphorylation modification treatment comprises placing the formed basic calcium-source microspheres in a phosphorylation reagent system for directional surface modification, continuously stirring at 300-500 r / min for 1-2 h during modification, and after completion, washing with deionized water and drying at low temperature to obtain the hydrophilic calcium-source modified microsphere component.

9. The GAB A-ly sine calcium supplement preparation for promoting calcium absorption according to claim 7, characterized in that in the preparation of the composite inclusion compound, carboxymethyl- P-cyclodextrin is addedCLAIMS to deionized water to prepare a saturated aqueous solution, and GABA, L-lysine, and casein phosphopeptide are then added; the saturated aqueous solution method is employed in a constant-temperature environment of 40-50°C with continuous stirring at 200-400 r / min for 2-3 h, followed by suction filtration and drying to obtain the absorption-promoting inclusion component having an inclusion rate of >95%.

10. The GAB A-ly sine calcium supplement preparation for promoting calcium absorption according to claim 7, characterized in that in the preparation of the deposition component, natto and Agaricus bisporus are separately pulverized, and K2MK7 and natural VD2 are separately extracted by a supercritical CO2 extraction method under an extraction pressure of 30-40 MPa, an extraction temperature of 45-55°C, and an extraction time of 2-3 h; K2MK7 and natural VD2 are then mixed and subjected to chromatographic purification to obtain the calcium-directed deposition component having a purity of >98%.

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