Cosmetic composition containing perilla frutescens extract

Abstract

The present invention relates to a cosmetic composition comprising a Perilla frutescens extract. The Perilla frutescens extract of the present invention can exhibit excellent effects of inhibiting lipid production in the skin, reducing sebum secretion, anti-inflammatory effects, and the like, and thus can be widely used as a component of a cosmetic composition.

Description

Cosmetic composition containing millet extract

[0001] The present invention relates to a cosmetic composition comprising a millet extract.

[0002] This application claims priority based on Korean Patent Application No. 10-2024-0197571 filed on December 26, 2024, and all contents described in the specification and drawings of said application are incorporated by reference into this application.

[0003] Sebum is a fatty complex consisting of triglycerides, free fatty acids, squalene, and wax esters. It forms a thin film of oil on the stratum corneum of the skin and on the surface of the hair, protecting the skin from harmful external environments, preventing moisture loss, and maintaining internal moisture. Additionally, it helps maintain the skin's oil-water balance in conjunction with moisture from sources such as sweat.

[0004] It is known that sebum secretion is influenced by genetic factors, hormonal effects, stress, and dietary habits such as the intake of fats and carbohydrates, and it is produced in sebaceous glands distributed throughout the body, excluding the palms and soles, and secreted to the skin surface through pores.

[0005] When sebum secretion increases, it causes skin problems such as oily coating and crusts or scales on the skin. Excessive sebum secretion leads to an imbalance between sebum production and secretion ability, and sebum that is not sufficiently expelled can cause acne through a chain reaction of inflammation triggered by acne bacteria, which are commensal bacteria on the skin.

[0006] Acne is an inflammatory disease of the sebaceous glands, hair follicles, and surrounding tissues caused by the action of hormones, excessive sebum secretion, or bacterial infection, and can occur not only on the face but also on areas where sebaceous glands are developed, such as the chest, back, and armpits.

[0007] Skin troubles are not only inconvenient but also a major cause of decreased self-confidence regarding one's appearance; consequently, there is a growing demand for the development of products based on effective natural ingredients that can suppress lipid production and regulate sebum while minimizing skin irritation.

[0008] [Prior Art Literature]

[0009] [Patent Literature]

[0010] Korean Registered Patent No. 10-2411893

[0011] Korean Registered Patent No. 10-1852510

[0012]

[0013] Accordingly, the inventors of the present invention conducted research and efforts to discover a natural substance capable of inhibiting lipid production and reducing sebum secretion in the skin. As a result, they discovered that perilla extract exhibits excellent effects in inhibiting lipid production, reducing sebum secretion, and anti-inflammatory effects in the skin, thereby completing the present invention.

[0014] Therefore, the present invention aims to provide a cosmetic composition comprising a millet extract.

[0015]

[0016] The present invention provides a cosmetic composition for alleviating acne comprising a millet extract.

[0017] In addition, the present invention provides a cosmetic composition for inhibiting lipid production and reducing sebum secretion of the skin, comprising a millet extract.

[0018] The above millet extract may be extracted using water, C1-C4 alcohols, or a mixture thereof as a solvent.

[0019] The above millet may be Samda millet.

[0020]

[0021] The perilla extract of the present invention can exhibit excellent effects such as inhibiting lipid production in the skin, reducing sebum secretion, and anti-inflammatory effects, and can therefore be widely used as a component of cosmetic compositions.

[0022]

[0023] The present invention will be described in detail below. Prior to this, terms and words used in this specification and claims should not be interpreted as being limited to their ordinary or dictionary meanings. Instead, based on the principle that the inventor can appropriately define the concepts of terms to best describe their invention, they should be interpreted in a meaning and concept consistent with the technical spirit of the present invention. Therefore, the configurations described in the embodiments of this specification are merely one preferred embodiment of the present invention and do not represent all aspects of the technical spirit of the present invention. It should be understood that various equivalents and modifications capable of replacing them may exist at the time of filing this application.

[0024] The present invention features a cosmetic composition for alleviating acne comprising a millet extract.

[0025] The above-mentioned *Chajo* (Setaria italica Beauv.) is a type of millet grain. In this invention, *Chajo* is a native variety of *Chajo* that grows naturally in the Jeju region, and while *Samda Chajo* with the variety name *Samdachal* (refer to the National Seed Center variety), it is not limited thereto.

[0026] The above "extract" can be obtained using a conventional extraction solvent known in the art. Polar solvents or non-polar solvents may be used as extraction solvents. Polar solvents include water, C1 to C4 alcohols (e.g., methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, and normal-butanol, etc.), acetic acid, or mixtures of the above polar solvents. Non-polar solvents include acetone, acetonitrile, ethyl acetate, methyl acetate, butyl acetate, fluoroalkanes, hexane, ether, chloroform, dichloromethane, or mixtures of the above non-polar solvents.

[0027] The above extract may be extracted using water, C1 to C4 alcohol, or a mixture thereof as a solvent. Preferably, the solvent may be 1 to 90 weight% ethanol, more preferably 40 to 80 weight% ethanol, and most preferably 65 to 75 weight% ethanol.

[0028] The extract of the present invention may be obtained through hot water extraction, cold maceration extraction, reflux cooling extraction, ultrasonic extraction, or conventional extraction methods known in the art.

[0029] The term "extract" used in the present invention refers to what is commonly known as a crude extract in the art, but in a broader sense, it also includes fractions obtained by further fractionating the extract. That is, the extract includes not only that which is obtained using the solvent described above, but also that which is obtained by additionally applying a purification process thereto. For example, fractions obtained through various additional purification methods, such as a fraction obtained by passing the extract through an ultrafiltration membrane having a certain molecular weight cut-off value, or separation by various chromatographs (designed for separation based on size, charge, hydrophobicity, or affinity), are also included in the extract of the present invention.

[0030] The cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions, such as, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances, and may include conventional auxiliary agents and carriers.

[0031] The cosmetic composition of the present invention can be prepared in any formulation conventionally manufactured in the industry, and may be formulated, for example, as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray, but is not limited thereto.

[0032] More specifically, it can be manufactured in the form of a softening lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

[0033] In the case where the formulation of the present invention is a paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.

[0034] In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane / butane, or dimethyl ether.

[0035] When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent, or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

[0036] In the case where the formulation of the present invention is a suspension, liquid diluents such as water, ethanol, or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used as carrier components.

[0037] In the case where the formulation of the present invention is a cleansing agent containing a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester, etc. may be used as a carrier component.

[0038] When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be wiped off, peeled off, or washed off with water after application to the skin. As specific examples, the soap is a liquid soap, powder soap, solid soap, and oil soap; the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack; and the surfactant-free cleansing formulation is a cleansing cream, cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

[0039] In addition, the above-mentioned millet extract may be included in a pharmaceutical composition for external use on the skin, and the pharmaceutical composition may be in the form of a cream, gel, patch, spray, ointment, warning agent, lotion, liniment, paste, or cataplasma, but is not limited thereto.

[0040]

[0041] Hereinafter, the present invention will be described in detail through examples and experimental examples to specifically explain the invention. However, the embodiments according to the present invention may be modified in various different forms, and the scope of the present invention should not be interpreted as being limited to the embodiments described below. The embodiments of the present invention are provided to more completely explain the invention to those with average knowledge in the art.

[0042]

[0043] Example 1: Preparation of Millet Extract

[0044] Millet was purchased from a milled variety grown in Hankyeong-myeon, Jeju-si (Korea) and used in the experiment. Millet was reflux-extracted with a 70% (w / w) ethanol solution at 60°C, cooled, and filtered using No. 2 filter paper (Whatman, UK). The filtered extract was concentrated under reduced pressure (HS-5SP, HANSHIN S&T, Korea) and freeze-dried (FD 8518, IlShinBioBase, Korea) to produce millet powder, which was then stored at -20°C.

[0045]

[0046] Example 2: Preparation of Millet Extract

[0047] A millet extract was obtained in the same manner as in Example 1, except that the millet was reflux-extracted with a 30% (w / w) ethanol solution.

[0048]

[0049] Example 3: Preparation of Millet Extract

[0050] A millet extract was obtained in the same manner as in Example 1, except that the millet was reflux-extracted with a 90% (w / w) ethanol solution.

[0051]

[0052] Comparative Example: Preparation of Meso Extract

[0053] A mejo extract was obtained by proceeding in the same manner as in Example 1 above, except that instead of the above-mentioned foxtail millet, mejo grown in Yecheon, Gyeongbuk was reflux-extracted with a 70% (w / w) ethanol solution at 60°C.

[0054]

[0055] Test Example 1: Confirmation of lipid production inhibitory effect

[0056] Sebocytes in a 24-well plate: 3.0 × 10 4 Cells were seeded into cells / well and pre-cultured for 48 hours. Afterward, the medium was exchanged with serum-free medium containing 100 μM palmitic acid and the sample of the above example or comparative example, and cultured for 48 hours. After culture, the supernatant was removed, and the cells were washed with phosphate buffered saline (PBS, Sigma, USA) and treated with 0.05% Trypsin-EDTA to collect the cells. The lipid content of the collected cells was measured using a lipid extraction kit (Abcam, UK) and a lipid assay kit (Abcam, UK).

[0057] Classification Concentration (ug / ml) Lipid Formation Inhibition Rate (%) Example 15 17.2 ± 2.5 10 35.9 ± 3.7 20 39.4 ± 2.7 Example 2 20 14.2 ± 5.6 Example 3 20 14.4 ± 3.3 Comparative Example 2 20 13.7 ± 1.3

[0058] As shown in Table 1 above, when the sample of Example 1 was treated at a concentration of 20 μg / ml, it was confirmed that the best lipid production inhibition effect was observed, and as the treatment concentration increased, a higher lipid production inhibition effect was observed.

[0059]

[0060] Test Example 2: Confirmation of anti-inflammatory improvement effect

[0061] Confirmation of nitric oxide (NO) production inhibitory activity

[0062] 1.5 × 10⁶ RAW 264.7 cells in a 24-well plate 5 Cells were dispensed at a density of cells / mL and cultured for 18 hours at 37°C under 5% CO2 conditions. The solution was then replaced with DMEM containing 10% fetal bovine serum (FBS), treated with the prepared extract, and cultured for 24 hours. 100 µL of the cell culture supernatant was collected into a 96-well plate, 100 µL of Griess reagent was added, and the mixture was reacted at room temperature for 10 minutes. Subsequently, the absorbance was measured at 540 nm using a microplate reader, and the results are shown in Table 2 below.

[0063] Classification Concentration (ug / ml) NO Production Inhibition Rate (%) Example 1 50 21.6 ± 1.8 100 27.0 ± 2.2 200 30.2 ± 3.8 Example 2 200 11.5 ± 1.2 Example 3 200 8.7 ± 5.6 Comparative Example 2 200 13.0 ± 2.2

[0064] As shown in Table 2 above, when the sample of Example 1 was treated at a concentration of 200 μg / ml, it showed the best NO production inhibition effect, and it was confirmed that as the treatment concentration increased, it showed a higher NO production inhibition effect.

Claims

1. A cosmetic composition for alleviating acne characterized by containing millet extract.

2. A cosmetic composition for inhibiting lipid production and reducing sebum secretion in the skin, characterized by containing a millet extract.

3. In Paragraph 1 or 2, A cosmetic composition characterized by the above-mentioned millet extract being extracted using water, C1-C4 alcohol, or a mixture thereof as a solvent.

4. In Paragraph 1, A cosmetic composition characterized in that the above-mentioned millet is Samda millet.