Isolating a membrane protein using a native cell membrane nanoparticle

The NCMN system addresses the challenge of maintaining TSPO's structure and function by using a polymer-based method, allowing for efficient isolation and drug screening without detergents, thus enhancing our understanding of TSPO's role and facilitating drug design.

WO2026151828A1PCT designated stage Publication Date: 2026-07-16VIRGINIA COMMONWEALTH UNIV
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Patent Information

Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
VIRGINIA COMMONWEALTH UNIV
Filing Date
2026-01-08
Publication Date
2026-07-16

AI Technical Summary

Technical Problem

Existing methods for isolating tryptophan-rich sensory protein (TSPO) fail to maintain its structural integrity and functional activity, leading to uncertainties in understanding its physiological and pathological roles and hindering structure-based drug design.

Method used

A Native Cell Membrane Nanoparticle (NCMN) system is used to extract and isolate TSPO, employing a specific polymer structure that preserves the native lipid environment and avoids detergents, ensuring the protein's native structure and activity are maintained.

Benefits of technology

The NCMN system effectively isolates TSPO with its native structure and function intact, enabling further analysis and drug screening applications while tolerating low pH conditions and eliminating the need for detergent use.

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Abstract

Provided are methods of isolating or extracting human mitochondrial tryptophan-rich sensory protein (HsTSPOl). The methods include contacting the H.sTSPOl with an effective amount of at least one Native Cell Membrane Nanoparticle (NCMN) polymer and recovering the TSPO. Compositions containing TPSO encapsulated in a particle formed by the NCNM polymer are also provided. A protoporphyrin derivative compound, methods of producing bilindigin or derivatives thereof, and methods of screening compounds that modulate TSPO activity are also provided.
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