The invention discloses an assembling device of a biological reagent plate. The assembling device comprises a machine table, a rotating conveying device fixed to the machine table, a lower shell feeding device, a reagent strip hobbing feeding device, a reagent strip assembling detecting device, an upper shell feeding device, an upper and lower shell press-fit device, a press-fit height detection device and a discharging device, wherein the lower shell feeding device, the reagent strip hobbing feeding device, the reagent strip assembling detecting device, the upper shell feeding device, the upper and lower shell press-fit device, the press-fit height detection device and the discharging device surround the edge of the rotating conveying device and are arranged according to the clockwise or counter-clockwise order. Automatic feeding of an upper shell and a lower shell and a series of full-automatic actions such as automatic slitting, automatic assembling, automatic detection and automatic discharging of reagent strips are achieved, and six reagent strips can be cut at a time; 80 to 90 products can be produced in each minute, the product assembling production efficiency is greatly improved, and meanwhile labor cost is reduced.