The invention discloses a universal test strip for
rapid detection of multiple
nucleic acid amplification products as well as a preparation method and application of the universal test strip, and belongs to the technical field of
rapid detection of
nucleic acid. The universal test strip comprises a reaction bottom plate, a sample pad, a combination pad, a
chromatography membrane and a water absorption pad, wherein the combination pad is loaded with a marked first target
antibody, a marked first
quality control antibody or
antigen; the
chromatography membrane is provided with a detection line T coated with a second target
antibody and a
quality control line C coated with a second
quality control antibody or
antigen; the detection line and the quality
control line are sequentially arranged on the
chromatography membrane in parallel at intervals along the chromatography direction. The universal test strip has the remarkable advantages of simplicity and convenience in operation, high detection speed, high sensitivity, strong specificity, low cost and the like, is wide in application technical range, is suitable for various
nucleic acid detection technical methods, has strong tolerance to sample inhibitors, and is particularly suitable for being combined with isothermal amplification
rapid detection technologies such as RPA (
recombinase polymerase amplification), LAMP (loop-mediated isothermal amplification) and the like.