The invention discloses calamine lotion with a bacteriostatic effect. The calamine lotion is prepared from the following raw materials in parts by weight: 140-160 parts of modified calamine, 45-55 parts of zinc oxide, 5-7 parts of modified nano zinc oxide, 8-10 parts of officinal phenol, 6-8 parts of antibiotic, 10-12 parts of menthol, 20-26 parts of tween 20, 7-10 parts of glycerinum, 2-3 parts of sodium carboxymethylcellulose, and 800-900 parts of purified water. The calamine lotion disclosed by the invention takes the modified calamine and the zinc oxide as main effective ingredients of thelotion, wherein the anti-bacterial, convergent, antiseptic and antipruritic effects of the both ingredients are strong; through adding a proper amount of the modified nano zinc oxide, the lotion is endowed with good antibacterial performance; the officinal phenol is capable of prolonging a using expiration date of a preparation, increasing stability of the preparation, and enhancing the sterilizing, bacteriostatic and antipruritic effects of the preparation; in addition, in a preparation process of the calamine lotion, a sodium carboxymethylcellulose suspending agent is added to increase thestability of suspension; and the calamine lotion with the bacteriostatic effect, stable characters and a strong physiological property is prepared.