METHOD FOR ANALYZING THE DEGREE OF SIMILARITY OF AT LEAST TWO SAMPLES USING DETERMINISTIC RESTRICTION SITE WHOLE GENOME AMPLIFICATION (DRS-WGA)

Claims

A method for analyzing the degree of similarity of at least two samples in a plurality of samples comprising genomic DNA. The method comprises the following steps: a) Providing a plurality of samples comprising genomic DNA. b) Performing, separately on each sample, deterministic restriction site whole-genome amplification (DRS-WGA) of said genomic DNA. c) Preparing a massively parallel sequencing library using a PCR reaction with sequencing / WGA adapters using fusion primers, without fragmentation, from each product of said DRS-WGA. d) Performing low-pass whole-genome sequencing at an average depth of coverage of <1x on said massively parallel sequencing library. e) Aligning, for each sample, the reads obtained in step d) on a reference genome. f) Extracting, for each sample, the allelic content at a plurality of polymorphic loci.g) Calculate a pairwise similarity score for the at least two samples based on the allelic content measured at that plurality of loci. h) Determine the degree of similarity of the at least two samples based on the similarity score.