Mechanical perfusion preservation apparatus for a biological organ

Abstract

The present application relates to a kind of medical equipment, propose a kind of mechanical perfusion preservation device of organism organ, comprising: organ bin (2) is used to accommodate organism organ and accessory blood vessel;Liquid storage bin (3) is located below the organ bin (2), for storing circulating perfusion fluid;Temperature maintaining device (4) is used to maintain the temperature of organism organ and circulating fluid;Oxygenator (5) is used to continuously oxygenate circulating perfusion fluid;Filter plug device (6) is used to filter circulating perfusion fluid;Pump (7) is pressurized to circulating perfusion fluid;Perfusion pipeline (8) is connected in sequence liquid storage bin (3), filter plug device (6) and oxygenator (5), and under the drive of pump (7), the organism organ is pressurized, circulating perfusion;The mechanical perfusion preservation device can pressurize, circulating perfusion to the aortic system of organism, can simultaneously perfuse single or multiple organism organs.The device of the present application is based on the principle of organ mechanical perfusion, using oxygenated blood or acellular component circulating fluid, multiple organs can be simultaneously perfused.

Description

Technical Field

[0001] This invention relates to a medical device, and more specifically, to a mechanical perfusion preservation device for biological organs. Background Technology

[0002] With the rapid development of organ transplantation technology, the requirements for organ preservation technology are becoming increasingly stringent. Traditional cryopreservation in UW fluid can no longer meet clinical needs. Therefore, novel mechanical perfusion preservation technology has gradually been accepted by organ transplant centers worldwide. The optimal mechanical perfusion preservation device should meet the following characteristics: 1) Portable device for easy transport during preservation; 2) Real-time, dynamic assessment of organ viability during preservation; 3) Repair of damaged organs; 4) Simultaneous administration of drugs, gene editing, or stem cell infusion during preservation; 5) Simultaneous preservation of multiple organs; 6) Creating an in vitro environment that most closely resembles human physiology.

[0003] Current mechanical perfusion preservation techniques can only preserve single organs. For example, Organ-OX and Liver-assist can only preserve the liver, Kidney-assist and Life-Port can only preserve the kidney, and Trans Medics can only preserve the heart. However, during organ procurement from donated donors, each donor needs to have one liver, two kidneys, one pancreas, one heart, two lungs, and one small intestine—a total of eight organs. This requires multiple different organ preservation devices, which are not only complex and large but also difficult to operate, maintain, and transport simultaneously. Therefore, a universal preservation device capable of preserving all these organs at once is needed. Furthermore, human organs are interdependent; simultaneous preservation of multiple organs helps to leverage their mutual protective functions.

[0004] Furthermore, in medical training, to make training in surgical procedures such as laparoscopy, natural orifice endoscopy, surgical robots, and internal medicine digestive endoscopy more realistic, it is necessary to use large animals for surgical operation training. However, with the progress of social civilization and the development of medical ethics, coupled with the high cost of establishing large animal operating rooms and the expensive cost of a single large animal experiment, the use of large animals for surgical training has been limited. Currently, most medical training uses commercially available edible large animal organs for training purposes. However, these organs are often non-vitalized, ex vivo, dead organs, and are single organs. Not only do they lack vitality, but their state differs significantly from that of a living organ, and they cannot accurately simulate bleeding, intestinal peristalsis, liver bile production, kidney urine production, or heart beating. Therefore, the simulation effect is poor. Moreover, the overall anatomical structure of a single organ has been destroyed, making systematic anatomical training impossible, resulting in poor training effectiveness. Virtual reality training devices that have emerged in recent years also have many shortcomings, such as a lack of anatomical variation, a lack of realism in surgical operations, and high prices.

[0005] Chinese invention patent 201380046850.9 discloses an organ perfusion device with downstream flow control. Claim 1 of this invention mentions: a method for perfusing an organ or tissue, the method comprising: connecting a conduit to an organ or tissue, the conduit comprising multiple tubes, and a first end of each of at least two of the tubes being connected to the organ or tissue; applying a fluid motive force to perfusion fluid in the two tubes to force the fluid through the two tubes into the organ or tissue for perfusion; allowing a flow balance to be established between the two tubes by the fluid motive force and a back pressure generated by the perfused organ or tissue; and then changing the flow balance between the two tubes without changing the applied fluid motive force. This invention patent differs significantly in its basic structure from the present invention. The invention patent has a multi-tube structure, does not include an oxygenator and a filter plug, and the main components are all different.

[0006] Chinese invention patent application 201580002264.3 discloses a human body simulation device, comprising: an organ from an animal, having biological tissue and blood vessels extending from the biological tissue; a blood reservoir for storing blood to be transported to the blood vessels; a tube connecting the blood vessels and the blood reservoir; a pump located midway through the tube and pumping blood from the blood reservoir to the blood vessels; and a receiving section for receiving the organ, supplying steam at a temperature higher than room temperature to the organ to humidify it, and setting the organ at a temperature of 20-50°C. The basic structure of this invention does not include an oxygenator and a thrombectomy device, and it uses a single organ, making simultaneous perfusion of multiple organs impossible. Furthermore, this prior art achieves unidirectional perfusion, while human organs require blood return circulation. Therefore, existing organ mechanical perfusion devices are complex, bulky, and neither can nor achieve multi-organ preservation. Summary of the Invention

[0007] To achieve simultaneous ex vivo preservation of multiple organs in clinical practice, a multi-organ perfusion system centered on the abdominal or thoracic aorta is essential. To simplify operation, the device of this invention uses a single pump and perfusion tubing to simultaneously perfuse multiple organs or a single organ via the abdominal or thoracic aortic system. The portal vein of the liver does not require cannulation, relying on the circulating perfusion fluid through the intestinal artery system and splenic artery for return perfusion. Multiple thoracic organs can be simultaneously perfused via the thoracic aortic system, with the pulmonary artery requiring no cannulation, perfused via the circulating perfusion fluid from the right heart. In the case of preserving multiple organs, only a single cannula is needed, significantly simplifying the process, which is fundamentally different from existing technologies.

[0008] This invention discloses a mechanical perfusion preservation device for biological organs, comprising:

[0009] Organ compartment: Used to house the organs and associated blood vessels of an organism;

[0010] Storage tank: Located below the organ compartment, used to store circulating perfusion fluid;

[0011] Temperature maintenance device: used to maintain the temperature of biological organs and circulating fluids;

[0012] Oxygenator: Used to continuously oxygenate the circulating perfusion fluid;

[0013] Filter plug: Used for filtering circulating perfusion fluid;

[0014] Pump: Used to pressurize the circulating injection fluid;

[0015] Infusion pipeline: connected in sequence to the storage tank, filter plug and oxygenator, and pressurized and circulated infusion of the biological organs under the drive of the pump;

[0016] The mechanical perfusion preservation device can pressurize and circulate perfusion of the aortic system of an organism, and can simultaneously perfuse one or more organs of an organism.

[0017] Furthermore, the mechanical perfusion preservation device can simultaneously perfuse multiple abdominal organs through the abdominal aortic system, wherein the portal vein of the liver does not require cannulation and is perfused through the circulating perfusion fluid returning from the portal vein system.

[0018] Furthermore, the mechanical perfusion preservation device can simultaneously perfuse multiple thoracic organs through the thoracic aortic system, wherein the pulmonary artery does not require cannulation and is perfused through the circulating perfusion fluid of the right heart.

[0019] Furthermore, the perfusion preservation device also includes a vascular cannula, which is located at the end of the perfusion tubing and is used to connect to the accessory blood vessels of biological organs for perfusion.

[0020] Furthermore, the vascular cannula has corresponding side holes, the inner diameter of which matches the position and inner diameter of the vascular cannula in different branches of the aortic system.

[0021] Furthermore, the temperature maintaining device is a water bath, and the organ compartment and the liquid storage compartment are placed inside the water bath.

[0022] Furthermore, the infusion and preservation device also includes an oxygenator heating circulation pump, which is used to extract warm water from the water bath to circulate and heat the oxygenator.

[0023] Furthermore, the infusion preservation device also includes a temperature maintenance device comprising a temperature controller capable of controlling the temperature of the water bath.

[0024] Furthermore, the perfusion preservation device also includes a temperature maintenance device, which is a compressor, ice, or cryogenic agent, used to maintain the low temperature of the liquid storage chamber, organ chamber, and biological organs.

[0025] Furthermore, the perfusion preservation device also includes a temperature sensor for monitoring the temperature of the circulating perfusion fluid and biological organs within the storage tank;

[0026] A flow sensor is used to monitor the flow rate of the circulating injection fluid in the injection pipeline;

[0027] Pressure sensor used to monitor the pressure of circulating injection fluid in the injection pipeline;

[0028] A bubble sensor is used to monitor for air bubbles in the injection line.

[0029] Furthermore, the perfusion preservation device also includes a controller that collects signals from temperature sensors and controls a temperature maintenance device based on the signals.

[0030] Furthermore, the infusion preservation device also includes a controller that collects signals from the flow sensor and the pressure sensor, and controls the pump based on the signals.

[0031] Furthermore, the infusion preservation device also includes a controller that collects signals from a flow sensor, a pressure sensor, and a bubble sensor, and controls an infusion line clamping valve based on the signals, the infusion line clamping valve being able to stop the infusion of the infusion line.

[0032] Furthermore, the organ compartment has an organ compartment shelf at the bottom, and the organ compartment shelf has through holes that communicate with the liquid storage compartment.

[0033] Furthermore, the perfusion preservation device also includes a bile collection and metering device for collecting bile and a urine collection and metering device for collecting urine, so as to dynamically observe the vitality of the organism's organs.

[0034] Compared with the prior art of Chinese Patent Application 201580002264.3, the present invention differs in the following ways: 1) The basic structure is different. The device of the present invention includes not only organs and their connecting blood vessels, pump, tube, heating device, and organ compartment, but also oxygenator, embolizer, etc.; 2) The tubing structure of the device of the present invention is different from that of Chinese Patent Application 201580002264.3. The present invention uses a single pump and a single blood vessel cannulation to simultaneously perfuse multiple organs in the abdominal or thoracic cavity through the abdominal aorta or thoracic aorta system. The portal vein of the liver does not require cannulation and relies on the intestinal and spleen reflux perfusion fluid for perfusion. The device can simultaneously perfuse multiple thoracic organs through the thoracic aorta system, wherein the pulmonary artery does not require cannulation and is perfused through the right heart circulation perfusion fluid; 3) The present invention realizes blood reflux circulation, while Chinese Patent Application 201580002264.3 only describes unidirectional perfusion and does not mention circulatory perfusion.

[0035] In addition, the beneficial effects of the present invention also include:

[0036] 1) The device of this invention is suitable for clinical organ preservation, repair, and viability assessment. It involves inserting a single catheter into the abdominal or thoracic aorta and its branches, simultaneously perfusing and preserving multiple organs. The portal vein of the liver does not require catheterization, relying on blood return from the intestinal arterial system and splenic artery for perfusion; the pulmonary artery does not require catheterization, perfusing via blood from the right ventricle. This allows for full utilization of the mutual protective functions of multiple organs, such as the kidneys' excretion of excess water and maintenance of acid-base balance, the liver's metabolic and immune functions, the intestines' functions of absorbing water and nutrients and promoting liver repair, and the pancreas' functions of regulating blood sugar and promoting digestion. These multiple organs work synergistically to reduce the risk of graft dysfunction or failure after organ transplantation.

[0037] 2) The device of this invention is also applicable to minimally invasive medical training (laparoscopy, natural orifice endoscopy, surgical robots, digestive endoscopy, etc.). It utilizes the simple structure of a single-cannula aortic system for perfusing multiple organs, and uses discarded large animal organ clusters obtained from pig farms for in vitro perfusion preservation. Blood or cell-free circulating perfusion fluid is used to replenish energy substrates and oxygen, maintaining organ viability without damaging the anatomical structure of abdominal or thoracic organs. This helps doctors master the basic operations of routine surgeries or minimally invasive techniques. While closely approximating the effects of large animal experiments, it has significant advantages such as reduced costs, avoidance of ethical issues, preservation of in vivo anatomical structures despite anatomical variations, organ viability, and minimal bleeding after injury. Attached Figure Description

[0038] Figure 1 This is a schematic plan view of the basic structure of the first embodiment of the device of the present invention.

[0039] Figure 2This is a three-dimensional schematic diagram of the basic structure of the first embodiment of the device of the present invention.

[0040] Figure 3 This is a schematic diagram illustrating the basic principle of the first embodiment of the device of the present invention.

[0041] Figure 4 This is a top view of the organ compartment in the second embodiment of the device of the present invention.

[0042] Figure 5 This is a top view of the core component of the second embodiment of the device of the present invention.

[0043] Figure 6 This is a side view of the core component of the second embodiment of the device of the present invention.

[0044] Figure 7 This is an exploded view of the core components of the second embodiment of the device of the present invention.

[0045] Figure 8 This is the overall appearance of the oxygenator and filter assembly in the second embodiment of the device of the present invention.

[0046] Figure 9 This is a cross-sectional view of the oxygenator and filter assembly according to the second embodiment of the device of the present invention.

[0047] Figure 10 This is a schematic diagram of the electronic control system of the second embodiment of the device of the present invention.

[0048] Figure 11 This is a schematic diagram of a bile and urine collection device according to a second embodiment of the present invention.

[0049] Figure Labels

[0050] 1-Organ, 2-Organ compartment, 3-Liquid reservoir, 4-Temperature maintenance device, 5-Oxygenator, 6-Filter plug, 7-Pump, 8-Perfusion tubing, 9-Vascular cannula, 10-Water bath, 14-Diaphragm suspension, 16-Bile collection and metering device, 17-Urine collection and metering device, 22-Diaphragm, 23-Proximal abdominal aortic ligation suture, 24-Celiac trunk, 25-Splenic artery, 26-Superior mesenteric artery, 27-Distal abdominal aortic cannulation ligation suture, 28-Abdominal aorta, 29-Inferior and inferior vena cava, 30-Liver, 31-Proper hepatic artery, 32-Confluence of splenic vein, superior mesenteric vein, and portal vein, 33-Renal artery, 34-Inferior vena cava, 42-Temperature controller, 43-Lifting column 46-Oxygen cylinder, 54-Oxygenator and thimble housing, 58-Organ compartment shelf, 60-Recirculation line, 63-Oxygenator heating and circulation interface, 64-Vascular cannula connection, 65-Oxygenator heater, 66-Flow sensor, 67-Controller, 68-Temperature sensor, 69-Pressure sensor, 70-Perfusion line clamp valve, 71-Oxygenator heating and circulation pump, 72-Spleen, 73-Stomach, 74-Intestine, 75-Kidney, 76-Portal vein, 77-Splenic vein, 78-Superior mesenteric vein, 79-Inferior mesenteric vein, 80-Right gastroepiploic vein, 81-Inferior mesenteric artery, 82-Low-voltage DC power supply, 83-Control panel and display screen, 84-Alarm device, 85-Bubble sensor. Detailed Implementation

[0051] Embodiments of the present invention are described below with reference to the accompanying drawings, wherein like parts are indicated by like reference numerals. Unless otherwise specified, the following embodiments and technical features described herein can be combined with each other.

[0052] First Implementation Method

[0053] like Figure 1-3 As shown, the device of the present invention is used for a biological organ 1, which includes a biological organ and blood vessels attached to the biological organ. The biological organ 1 can be an organ cluster or a single organ.

[0054] The device of the present invention is used for mechanical perfusion preservation of biological organs 1, comprising: an organ compartment 2, a fluid storage compartment 3, a temperature maintenance device 4, an oxygenator 5, a thrombectomy device 6, a pump 7, and a perfusion line 8. Preferably, it includes a vascular cannula 9 at the end of the perfusion line 8.

[0055] Organ compartment 2 is used to house the biological organ 1 and the blood vessels attached to the biological organ; reservoir 3 is located at the lower part of organ compartment 2 and is used to collect the circulating perfusion fluid returning from the venous end of the biological organ; temperature maintenance device 4 is used to maintain the temperature of biological organ 1, organ compartment 2, reservoir 3 and the entire device; oxygenator 5 is used to oxygenate the circulating perfusion fluid, and oxygenator 5 is connected to oxygen cylinder 46; thrombus filter 6 is used to filter impurities such as thrombi and air bubbles in the circulating perfusion fluid; pump 7 is used to maintain the perfusion power of the device, for pressurized perfusion and circulating perfusion; perfusion tubing 8 is used to connect various key components; vascular cannula 9 is located at the end of perfusion tubing 8 and connects to the aortic system of biological organ 1 for pressurized perfusion and circulating perfusion. Preferably, vascular cannula 9 has corresponding side holes at different branches within the aortic system, with an inner diameter matching them, which allows for more accurate perfusion of the corresponding organs. Vascular cannula 9 may also have multiple branches.

[0056] The portal vein 76 does not require catheterization and relies on the return perfusion fluid from the splenic artery 25 to the spleen 72 and the return perfusion fluid from the superior mesenteric artery 26 to the intestine 74. The perfusion occurs after the splenic vein, superior mesenteric vein and portal vein converge at the junction 32.

[0057] like Figure 2 As shown, the basic working principle of this device is as follows: Organ 1 is placed inside organ compartment 2, and the temperature is maintained by a temperature maintaining device 4 on the outside of organ compartment 2 (organ compartment 2 is placed inside temperature maintaining device 4). The circulating perfusion fluid (including blood or non-cellular circulating fluid, etc.) flows back and is stored in a reservoir 3, which is located below organ compartment 2. The organ compartment shelf 58 of organ compartment 2 has through holes, allowing physical communication between organ compartment 2 and reservoir 3, facilitating the return of perfusion fluid from organ 1 to the reservoir 3 below organ compartment 2. In one embodiment (e.g.) Figure 6 , Figure 7 As shown, the temperature maintaining device 4 is in the form of a water bath 10, which can not only directly heat the organ compartment 2 immersed in the water bath 10, but also has an oxygenator heating circulation pump 71 (located inside or outside the water bath 10) to draw warm water from the water bath 10 and circulate it to heat the oxygenator 5, thereby improving heating efficiency. In another embodiment, the temperature maintaining device 4 is in the form of a compressor, ice, or a cryogenic agent to maintain the low temperature of the liquid storage compartment 3, the organ compartment 2, and the biological organs 1 inside.

[0058] Driven by pump 7, the circulating perfusion fluid in reservoir 3 enters filter 6 to filter out impurities and air bubbles; then it is oxygenated by oxygenator 5; then it enters the aortic system of biological organ 1 in organ compartment 2 through circulation pipeline 8 and vascular cannula 9, and then multiple organs are perfused simultaneously using different branches of the aorta; finally, the circulating perfusion fluid flows out through the venous end of biological organ 1 and is collected and returned to reservoir 3, thus forming a circulating perfusion.

[0059] The circulation sequence is as follows: Organoid 1 in Organ Chamber 2 — Liquid Storage Chamber 3 — Plug 6 — Pump 7 — Oxygenator 5 — Perfusion Line 8 (Vascular Cannula 9) — Organoid 1 in Organ Chamber 2. Perfusion Line 8 connects to each major component.

[0060] The organ preservation device of the present invention simultaneously perfuses multiple organs via the abdominal aorta or thoracic aortic system. The portal vein 76 of the liver does not require cannulation and is perfused via circulating perfusion fluid returning from the portal vein system. The pulmonary artery does not require cannulation and is perfused via circulating perfusion fluid from the right heart.

[0061] Optionally, the vascular cannula 9 is located at the end of the perfusion line 8 and is connected to the accessory blood vessels of the biological organ 1 for perfusion.

[0062] Optionally, the vascular cannula 9 has corresponding side holes at different branches within the aortic system, with matching inner diameters, allowing for more accurate perfusion of the corresponding organs.

[0063] like Figure 3As shown, when using this device, firstly, the biological organ 1 and its associated blood vessels are placed in the organ compartment 2, with all organs arranged according to normal anatomical structure. Then, the two ends of the abdominal aorta 28 are ligated with proximal abdominal aortic ligation 23 and distal abdominal aortic ligation 27, respectively. The circulation tubing 8 (optionally, with its end connected to a vascular cannula 9) is inserted into the abdominal aorta 28 and fixed with the ligation sutures. The circulating perfusion fluid is pressurized by the pump 7 and injected into the abdominal aorta 28 through the circulation tubing 8 (optionally, with its end connected to a vascular cannula 9), and then perfused into each organ through the branches of the abdominal aorta. The device perfuses both kidneys 75 via the renal artery 33; the liver 30 via the proper hepatic artery 31 (branch 24 of the celiac trunk); the spleen 72 via the splenic artery 25 (branch 24 of the celiac trunk); and the intestines 74 via the superior mesenteric artery 26 and inferior mesenteric artery 81. Venous return from the intestines 74 to the superior mesenteric vein 78 and inferior mesenteric vein 79, venous return from the spleen 72 to the splenic vein 77, and venous return from the stomach 73 to the right gastroepiploic vein 80, all converging at the junction 32 of the splenic vein, superior mesenteric vein, and portal vein. All these veins then perfuse the liver via the portal vein 76, thus eliminating the need for cannulation in the portal vein 76. This device can complete perfusion of all abdominal organs by perfusing the abdominal aorta 28 and its branches using only a single cannula. All circulating perfusion fluid flows back to organ compartment 2 and reservoir 3 through the inferior vena cava 29 and inferior vena cava 34 of the inferior vena cava system. Then, driven by pump 7, the circulating perfusion fluid in reservoir 3 enters filter 6 to filter out impurities and air bubbles. Then it is oxygenated by oxygenator 5. Then it enters the aortic system of biological organ 1 in organ compartment 2 through circulation tubing 8 (optionally, the end is connected to vascular cannula 9). Then, multiple organs are perfused simultaneously using different branches of the aorta, and this cycle is repeated.

[0064] This device can simultaneously perfuse multiple thoracic organs through the thoracic aortic system, with the pulmonary artery requiring no cannulation and perfused via the circulating perfusion fluid of the right heart.

[0065] Optionally, the vascular cannula 9 is located at the end of the perfusion line 8 and is connected to the accessory blood vessels of the biological organ 1 for perfusion.

[0066] Optionally, the vascular cannula 9 has corresponding side holes at different branches within the aortic system, with matching inner diameters, allowing for more accurate perfusion of the corresponding organs.

[0067] Optionally, the device of the present invention can be used for extracorporeal mechanical perfusion preservation of human organs, for the preservation of multiple organs, to play a key role in mutual protection, and to preserve, repair, and assess organ viability at the same time.

[0068] Optionally, the device of the present invention uses animal organs such as pigs or sheep, which are mechanically perfused in vitro to restore organ vitality, maximally simulating human organs for medical training.

[0069] Second Implementation Method

[0070] The following reference Figure 4-10 The second embodiment of the present invention is described, further detailing the structure and principle of the organ compartment 2, the fluid storage compartment 3, the temperature maintenance device 4, the oxygenator 5, and the filter plug 6.

[0071] like Figure 4 As shown, the organ compartment 2 has an organ compartment shelf 58 at the bottom with several holes, which connects to the lower liquid storage compartment 3 (see...). Figure 2 The organ chamber 2 is connected to the storage tank 3, facilitating the return of the circulating fluid to the storage tank 3. A return pipe 60 is installed inside the organ chamber 2, with one end leading to the storage tank 3 and the other end connected to the circulation pipe 8. Preferably, the bottom surface of the storage tank 3 is inclined towards the point where the return pipe 60 connects, which helps maintain a higher liquid level, facilitating fluid collection and preventing air intake through the return pipe 60. An oxygenator heating circulation interface 63 is installed on the outer wall of the organ chamber 2, used to draw warm water from the storage tank 3 below the organ chamber 2 and heat the circulating perfusion fluid via the oxygenator 5. The oxygenator heating circulation interface 63 is not connected to the organ chamber 2 but is connected to the water bath 10 (described in detail below).

[0072] A diaphragm suspension 14 is provided on the side wall of organ compartment 2 for suspending the diaphragm 22 (see...). Figure 1 The diaphragm suspension 14 is a protrusion extending inward from the inner wall of the organ compartment 2, and multiple diaphragm suspensions 14 can be provided. The diaphragm hook 14 is used to fix and suspend the diaphragm portion of the biological organ 1, so as to restore the anatomical position of the liver in the abdominal cavity to the greatest extent.

[0073] like Figures 5-7 As shown, the device of the present invention includes: an organ compartment 2, a water bath 10 (optionally a temperature maintenance device 4), a pump 7, an oxygenator 5, a thrombectomy 6, a return line 60, a vascular cannula connection 64, and a vascular cannula 9. The return end of the perfusion line 8 is connected to the return line 60, and the perfusion end is connected to the vascular cannula connection 64 and the vascular cannula 9. The pump 7, oxygenator 5, and thrombectomy 6 are connected in the middle of the perfusion line 8. The biological organ 1 is placed on the organ compartment shelf 58 within the organ compartment 2 and immersed together in the water bath 10 to maintain the body temperature environment. The diaphragm hook 14 is used to fix and suspend the diaphragm portion of the biological organ 1, maximizing the restoration of the intra-abdominal anatomical position. Optionally, the organ shelf 58 of the organ compartment 2 can be made of metal and serve as the negative electrode plate of the high-frequency electrosurgical unit 15 for use with the high-frequency electrosurgical unit 15.

[0074] Under the pressure of pump 7, the circulating perfusion fluid enters the abdominal or thoracic aortic system of organ 1 through vascular cannula 9, simultaneously perfusing multiple organs in the abdominal or thoracic cavity. The portal vein 76 does not require cannulation, relying on the return perfusion fluid from the splenic artery 25 to the spleen 72, and the superior mesenteric artery 26 receives the return perfusion fluid from the intestine 74. Perfusion occurs after the splenic vein, superior mesenteric vein, and portal vein converge at point 32. The pulmonary artery does not require cannulation, perfusing via the circulating perfusion fluid from the right heart. Subsequently, the circulating perfusion fluid flows out through the inferior vena cava of organ 1 and is collected and returned to the reservoir 3 at the bottom of organ 2 through the porous structure of organ compartment shelf 58. Then, driven by pump 7, the circulating fluid in reservoir 3 is drawn out through return pipeline 60 and flows to filter 6 to filter out thrombi and air emboli mixed in the circulating fluid. Then, through the outlet of filter 6, it passes through perfusion pipeline 8 and pump 7, and then enters oxygenator 5 through perfusion pipeline 8 to oxygenate the circulating fluid. Then, it enters vascular cannulation connection part 64 through the outlet of oxygenator 5, and then enters vascular cannulation 9 through vascular cannulation connection part 64. This process is repeated to complete the circulating perfusion process.

[0075] like Figures 6-9 As shown, organ compartment 2 and fluid storage compartment 3 are placed inside temperature maintenance device 4, which includes a water bath 10 and a temperature controller 42. Temperature is maintained through two methods. First, the water bath 10 contains warm water that directly heats organ compartment 2, fluid storage compartment 3, and the biological organ 1. Second, an oxygenator heating circulation pump 71 draws warm water from the water bath 10 through the oxygenator heating circulation interface 63 into the oxygenator heater 65, which is located below the oxygenator 5, to heat the circulating fluid within the oxygenator 5.

[0076] Temperature controller 42 can control the temperature of water bath 10, and temperature controller 42 can control the electric heating device to heat the water in water bath 10.

[0077] The oxygenator 5 and the embolus filter 6 are centrally located inside the oxygenator and embolus filter housing 54, and the corresponding circulation lines 8 are regularly distributed in the housing 54 for easy installation and replacement. Optionally, except for the pump 7, the organ compartment 2, the fluid storage compartment 3, the oxygenator 5, the embolus filter 6, the perfusion line 8, the vascular cannula 9, the return line 60 and other components can be disposable for easy replacement.

[0078] For reference again Figure 6Temperature sensor 68 is installed inside organ compartment 2 to monitor the temperature of the biological organ 1 within organ compartment 2. Flow sensor 66 is installed on the return tubing 60 and the vascular cannula connection 64 to monitor the flow rate of the circulating perfusion fluid through the return tubing 60 and the vascular cannula connection 64. Pressure sensor 69 is installed on the vascular cannula connection 64, the end of which is connected to the vascular cannula 9. Pressure sensor 69 monitors the pressure at the vascular cannula connection 64, that is, the pressure of the circulating perfusion fluid to be delivered to the vascular cannula 9. Bubble sensor 85 is used to monitor air bubbles within the perfusion tubing 8.

[0079] Refer again Figure 6 The organ perfusion preservation device of the present invention also includes a perfusion line clamping valve 70, which is disposed on the perfusion line 8. As shown in the figure, it is disposed near the input end of the pump 7. Closing the perfusion line clamping valve 70 can quickly stop the circulating perfusion of the perfusion line 8, so as to avoid damage to the biological organ 1 in case of abnormality.

[0080] like Figure 10 As shown, the organ perfusion preservation device of the present invention also includes an electronic control system powered by a low-voltage DC power supply 82. The electronic control system includes a controller 67, a control panel, and a display screen 83. Figure 10 The circuit diagram of the electronic control system is shown. The device of this invention may be equipped with a lifting column 43, which is installed below the liquid storage tank 3 to raise the height of the liquid storage tank 3 (together with the organ compartment 2). The device of this invention also includes a temperature sensor 68, a flow sensor 66, a pressure sensor 69, and a bubble sensor 85. The signals from these sensors are transmitted to the controller 67 for analysis and processing. The controller 67 controls the temperature of the temperature maintenance device 4, controls the speed of the pump 7 to prevent abnormal infusion pressure or flow, controls the opening and closing state of the infusion pipeline clamp valve 70, and provides timely alarm through the alarm device 84 when an abnormality occurs. The alarm can be displayed on the control panel and the display screen 83.

[0081] like Figure 11 As shown, the system of the present invention also includes a bile collection and metering device 16 and a urine collection and metering device 17, for collecting bile and urine and dynamically observing organ vitality.

[0082] The embodiments described above are merely preferred embodiments of the present invention. Ordinary variations and substitutions made by those skilled in the art within the scope of the technical solutions of the present invention should be included within the protection scope of the present invention.

Claims

1. A mechanical perfusion preservation device for biological organs, characterized in that, include: Organ compartment (2): Used to contain the organs (1) and associated blood vessels of a living organism; Storage tank (3): Located below the organ compartment (2), used to store circulating perfusion fluid; Temperature maintenance device (4): used to maintain the temperature of biological organs and circulating fluids; Oxygenator (5): Used to continuously oxygenate the circulating perfusion fluid; Filter plug (6): Used for filtering circulating perfusion fluid; Pump (7): pressurizes the circulating injection fluid; The perfusion line (8) is sequentially connected to the reservoir (3), the filter plug (6), and the oxygenator (5), and is pressurized and perfused under the drive of the pump (7). The mechanical perfusion preservation device can pressurize and circulate perfusion of the aortic system of an organism, and can simultaneously perfuse one or more organs of an organism. The mechanical perfusion preservation device can simultaneously perfuse multiple abdominal organs through the abdominal aortic system, wherein the portal vein (76) of the liver does not require cannulation and is perfused through the circulating perfusion fluid returning from the portal vein system. The mechanical perfusion preservation device can... Multiple thoracic organs are simultaneously perfused via the thoracic aortic system, with the pulmonary artery perfused without cannulation via the circulating perfusion fluid from the right heart; the temperature maintenance device (4) is a water bath (10), and the organ compartment (2) and the fluid storage compartment (3) are placed inside the water bath (10); the oxygenator heating circulation pump (71) is used to extract warm water from the water bath (10) to circulate and heat the oxygenator (5); the temperature sensor (68) is used to monitor the temperature of the circulating perfusion fluid and biological organs in the fluid storage compartment (3); the flow sensor... The device (66) is used to monitor the flow rate of the circulating perfusion fluid in the perfusion line (8); the pressure sensor (69) is used to monitor the pressure of the circulating perfusion fluid in the perfusion line (8); the bubble sensor (85) is used to monitor whether there are bubbles in the perfusion line (8); the controller (67) collects the signal from the temperature sensor (68) and controls the temperature maintaining device (4) based on the signal; the controller (67) collects the signals from the flow sensor (66) and the pressure sensor (69) and controls the pump (7) based on the signal; the controller (67) The system collects signals from the flow sensor (66), pressure sensor (69), and bubble sensor (85), and controls the perfusion line clamp valve (70) based on the signals. The perfusion line clamp valve (70) can stop the perfusion of the perfusion line (8). The vascular cannula (9) is located at the end of the perfusion line (8) and is used to connect to the accessory blood vessels of the biological organ (1) for perfusion. The vascular cannula (9) has corresponding side holes, and the inner diameter of the side holes matches the position and inner diameter of the vascular cannula (9) in different branches of the aortic system.

2. The perfusion preservation device according to claim 1, characterized in that, Also includes: The temperature maintaining device (4) includes a temperature controller (42) which is capable of controlling the temperature of the water bath (10).

3. The perfusion preservation device according to claim 1, characterized in that, Also includes: The temperature maintenance device (4) is a compressor, ice, or cryogenic agent used to maintain the low temperature of the liquid storage tank (3), organ tank (2), and biological organs.

4. The perfusion preservation device according to claim 1, characterized in that, The organ compartment (2) has an organ compartment shelf (58) at the bottom, and the organ compartment shelf (58) has a through hole that communicates with the liquid storage compartment (3).

5. The perfusion preservation device according to claim 1, characterized in that, Also includes: A bile collection metering device (16) for collecting bile and a urine collection metering device (17) for collecting urine, to dynamically observe the vitality of the organism's organs (1).

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