Multifunctional full-automatic colloidal gold detector

Abstract

The application provides a multifunctional full-automatic colloidal gold detector, which comprises a feeding mechanism, an X-axis sample adding mechanism, a TIP conveying mechanism, an incubation mechanism, a detector mounting table and a control device, a sample tube is mounted on the feeding mechanism, the feeding mechanism can add diluent into the sample tube and mix to form a sample to be used, a reagent card is placed on the incubation mechanism, and a TIP head is arranged on the TIP conveying mechanism; a TIP needle rod is arranged on the X-axis sample adding mechanism, the X-axis sample adding mechanism can install the TIP head on the TIP needle rod and can take the sample from the sample tube through the TIP head and drop the sample on the reagent card, the reagent card to which the sample is dropped is transported to an incubation bin for incubation, and detection is completed in the incubation mechanism after the incubation is completed, the detector is integrated into a full-automatic device, errors in manual operation are avoided, the workload of doctors is reduced, the detection accuracy is improved, and the detection efficiency is greatly improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical equipment, in particular to a multifunctional full-automatic colloidal gold detector. BACKGROUND

[0002] Colloidal gold method is that chloroauric acid (HAuCl4) can be polymerized into gold particles of certain size under the action of reducing agents such as white phosphorus, ascorbic acid, sodium citrate, tannic acid, etc., and become a stable colloidal state due to electrostatic interaction, forming a hydrophobic colloidal solution with negative charge. Because of the electrostatic interaction, it becomes a stable colloidal state, so it is called colloidal gold. Colloidal gold is also an ideal immunolabel in immunoelectron microscopy.

[0003] Most of the existing colloidal gold detection equipment is automatic equipment, but the function is relatively single. In the whole colloidal gold detection process, including sample drop diluent, sample mixing, sample adding to reagent card, reagent card incubation, reagent card detection and other steps, only the last step of reagent card detection is instrument detection, and other steps are manual operation. In comprehensive large hospitals, or in the high incidence season of infectious diseases, the manual workload is huge, and any operation error in the previous steps will affect the detection result. Therefore, to achieve the accuracy of detection, manual operation steps should be avoided as much as possible, which can reduce the risk of operation error and greatly reduce the workload of doctors and improve work efficiency.

[0004] Patent document CN209542625U discloses a colloidal gold detector, which comprises a sample inlet unit, an image collector, a screen display unit, a data processing unit and a control unit. The sample inlet unit comprises a colloidal gold detection card, a detection card fixator and a servo stepping motor. The detection card fixator is a hollow frame structure, and the colloidal gold detection card can be fixed on the detection card fixator and the bottom of the colloidal gold detection card is provided with a protrusion. The servo stepping motor rotates and drives the colloidal gold detection card to move under the image collector. A detection card groove is also provided below the image collector, and a groove matched with the protrusion on the colloidal gold detection card is provided on the detection card groove. A trigger sensor is arranged in the groove, and the sensor is connected with the data processing unit and the control unit. The control unit is connected with and controls the servo stepping motor and the image collector. However, this design does not design the steps of sample drop diluent, sample mixing, sample adding to reagent card, reagent card incubation, etc. In actual detection, the detection efficiency and detection quality cannot be guaranteed. SUMMARY

[0005] In view of the defects in the prior art, the purpose of the present application is to provide a multifunctional full-automatic colloidal gold detector.

[0006] The multifunctional full-automatic colloidal gold detector provided by the application comprises a feeding mechanism, an X-axis sample adding mechanism, a TIP conveying mechanism, an incubation mechanism, a detector mounting table and a control device.

[0007] The feeding mechanism, the X-axis sample adding mechanism, the TIP conveying mechanism and the incubation mechanism are all mounted on the detector mounting table.

[0008] The feeding mechanism comprises a sample rack, and a sample tube is mounted on the sample rack.

[0009] The incubation mechanism comprises a reagent card and an incubation bin.

[0010] The TIP conveying mechanism is provided with a TIP head.

[0011] The X-axis sample adding mechanism is provided with a TIP needle rod, and under the control of the control device, the X-axis sample adding mechanism can install the TIP head on the TIP needle rod, take the sample from the sample tube through the TIP head, add the sample to the reagent card, and then transport the reagent card with the added sample to the incubation bin for incubation, and finally complete the detection in the incubation mechanism.

[0012] Preferably, the feeding mechanism comprises a bearing rack, a first driving device, a second driving device, a liquid adding device, a squeezing device and a discharging device.

[0013] The bearing rack is provided with a bearing plate, and the bearing plate comprises a first feeding position, a liquid adding and squeezing position and a discharging position in sequence along the length direction.

[0014] The first driving device and the discharging device are respectively mounted on the first feeding position and the discharging position, and the liquid adding device and the squeezing device are respectively mounted on the liquid adding and squeezing position.

[0015] The second driving device is mounted on the bearing rack, and the second driving device is provided with a first transmission carrier and a second motor.

[0016] The first transmission carrier is provided with a bearing space at the upper end and is mounted on the bearing plate, and the lower end of the first transmission carrier extends to the lower side of the bearing plate through the first through hole and is drivingly connected with the second motor.

[0017] The first transmission carrier can drive the sample rack to move from the first feeding position to the liquid adding and extruding position and to the discharging position under the driving of the second motor, and the sample rack on the discharging position can be pushed out of the discharging position under the driving of the discharging device;

[0018] The sample rack comprises a sample rack base and a sample tube carrier;

[0019] The sample tube carrier is connected with the sample rack base through a plurality of connecting ribs;

[0020] A plurality of concave mounting grooves are arranged on the sample rack base in sequence and equidistantly, a plurality of cylindrical placement spaces are arranged on the sample tube carrier in sequence and equidistantly, the placement spaces and the mounting grooves correspond to each other in a one-to-one manner, and the sample tube is placed in the mounting grooves through the placement spaces, wherein one end of the connecting rib is arranged between two adjacent mounting grooves, the other end of the connecting rib is arranged between two adjacent placement spaces, and the width of the connecting rib is smaller than the diameter of the sample tube.

[0021] Preferably, the second driving device further comprises a first driving lead screw, a first bearing seat and a second bearing seat;

[0022] The first bearing seat and the second bearing seat are respectively arranged at two ends of the bottom of the bearing plate, the lower end of the first transmission carrier is provided with an internal thread hole, the first driving lead screw is provided with an external thread matched with the internal thread hole, one end of the first driving lead screw is arranged on the first bearing seat, the other end of the first driving lead screw sequentially passes through the internal thread hole, the second bearing seat and is drivingly connected with the second motor, and the driving mode of the second motor and the first driving lead screw comprises any one of the following structural forms:

[0023] - synchronous belt transmission is adopted;

[0024] - shaft coupling transmission is adopted;

[0025] - gear transmission is adopted;

[0026] The first driving device comprises a first motor, a protection plate, a first pulley, a first synchronous belt, a second pulley, a transmission member and a driving push plate;

[0027] The driving push plate is arranged on the upper surface of the sample rack storage area, the second pulley is arranged on the side surface of the sample rack storage area, and the transmission member is arranged on the first synchronous belt and connected with the driving push plate;

[0028] One end of the first synchronous belt is sleeved on the second pulley, the other end of the first synchronous belt is sleeved on the first pulley, and the first pulley is drivingly connected with the first motor;

[0029] When the first motor rotates, the first pulley is driven to rotate, the first synchronous belt is driven to move, and the transmission member drives the driving push plate to push the sample holder placed on the sample holder storage area into the bearing space.

[0030] The first motor and the first pulley are respectively installed on the two sides of the protection plate, the transmission member and the driving push plate are respectively installed on the two sides of the protection plate, the protection plate is provided with a second through hole, and the transmission member is connected with the driving push plate penetrating through the second through hole.

[0031] Preferably, the liquid adding device comprises a liquid adding motor and a sample adding assembly, the sample adding assembly is provided with a sample clamping arm, a peristaltic pump, a stepping motor and a sample adding needle, and the peristaltic pump is in driving connection with the stepping motor.

[0032] When the sample holder moves to the liquid adding and extruding position, the sample clamping arm clamping the sample adding needle can move above the sample holder under the driving of the liquid adding motor, and the liquid adding needle can complete liquid adding into the sample tube installed on the bearing frame through the peristaltic pump.

[0033] The extruding device comprises clamping heads, a fixing plate and springs.

[0034] The clamping heads and the fixing plate are both installed on the liquid adding and extruding position.

[0035] The number of the clamping heads is four, two of which are installed on one side of the first through hole, and the other two are installed on the other side of the first through hole, the other two clamping heads are connected with the fixing plate, and the interiors of the other two clamping heads are both installed with springs.

[0036] The clamping heads on the two sides correspond to each other, and a narrow channel is formed between each two corresponding clamping heads, the width of the narrow channel is smaller than the diameter of the sample tube.

[0037] The discharging device comprises a discharging motor, a discharging push rod, a second driving lead screw, a discharging base and discharging guide rods.

[0038] The discharging base is installed on the discharging position, the discharging motor is installed on the discharging base, two discharging through holes are respectively arranged on the discharging base and are respectively arranged on the two sides of the discharging motor, one end of each of the two discharging guide rods is installed on the discharging push rod, and the other end of each of the two discharging guide rods is installed in the two discharging through holes and can slide relative to the discharging through holes.

[0039] One end of the second driving lead screw is installed on the discharging push rod, and the other end of the second driving lead screw is connected with the discharging motor, when the discharging motor operates, the second driving lead screw drives the discharging push rod to move along the length direction of the second driving lead screw, and the two discharging guide rods move along with the discharging push rod and slide in the two discharging through holes.

[0040] Preferably, the incubation mechanism further comprises a reagent card conveying mechanism and a support plate;

[0041] The support plate is provided with a support plate, which comprises a discard position, a second feeding position, a detection position, a sample adding position and an incubation position in turn towards the direction close to the incubation bin, wherein the incubation position extends to the inside of the incubation bin;

[0042] The reagent card conveying mechanism comprises a conveying hook, which is installed on the support plate and can move between the discard position and the incubation position under the drive of the reagent card conveying mechanism;

[0043] The second feeding position is provided with a lifting plate and a first power component, the reagent card is placed on the lifting plate, the lifting plate can move between the first position and the second position under the drive of the first power component, and the first power component is driven by a motor or a cylinder;

[0044] When the lifting plate drives the reagent card to move to the first position, the conveying hook moves to the second feeding position under the drive of the reagent card conveying mechanism, at this time the lifting plate moves to the second position under the drive of the first power component, the reagent card enters the working range of the conveying hook, and the conveying hook can drive the reagent card to pass through the detection position, the sample adding position and enter the incubation position in turn;

[0045] The inside of the incubation bin is provided with an incubation bin, which comprises two incubation sub-bins and the two incubation sub-bins are respectively arranged on the two sides of the inside of the incubation bin along the width direction of the support plate, the incubation sub-bins comprise a plurality of incubation layer positions and the plurality of incubation layer positions are arranged in the incubation sub-bins in equidistant intervals in the up-down direction, the conveying hook entering the incubation position can drive the reagent card incubated on another incubation layer position to move to the detection position for detection, and after the detection is completed, the reagent card is finally transported to the discard position for discarding;

[0046] The incubation bin comprises an incubation bin shell and an incubation motor;

[0047] The incubation bin is installed in the inside of the incubation bin shell;

[0048] The inner wall of the incubation bin shell is provided with a first sliding rail in the height direction, the incubation bin is provided with a sliding block, and the sliding block is matched and installed on the first sliding rail;

[0049] The incubation motor is installed above the incubation bin shell, and the incubation motor is provided with an incubation driving rod;

[0050] The incubation driving rod extends through the top of the incubation bin shell to the inside of the incubation bin shell and is connected with the incubation bin, and the incubation site is provided with an incubation site through hole, and when the incubation motor operates, the incubation driving rod can be driven to move upward or downward, thereby driving the incubation bin to move upward or downward through the incubation site through hole under the guidance of the first sliding rail;

[0051] The detection site is provided with a darkroom and a camera;

[0052] The camera is mounted on the support plate through the darkroom;

[0053] The darkroom is mounted on the support plate and a third gap is provided between the darkroom and the support plate; the darkroom is provided with a shooting through hole, and the camera can shoot the reagent card placed on the detection site through the shooting through hole.

[0054] Preferably, the reagent card conveying mechanism further comprises a conveying guide rod, a second transmission carrier and a second power assembly;

[0055] The object table further comprises a third support leg and a fourth support leg, and the third support leg and the fourth support leg are respectively mounted at both ends of the lifting plate;

[0056] Both ends of the conveying guide rod are respectively mounted on the third support leg and the fourth support leg and are arranged below the lifting plate;

[0057] The second transmission carrier is provided with a transmission carrier through hole, the lower end of the second transmission carrier is sleeved on the conveying guide rod through the transmission carrier through hole and can slide relative to the conveying guide rod, and the upper end of the second transmission carrier is connected with the conveying hook;

[0058] The second power assembly can drive the second transmission carrier to slide along the direction of the conveying guide rod and simultaneously drive the conveying hook to slide on the lifting plate;

[0059] The second power assembly comprises a conveying motor, a third synchronous belt, a first driving synchronous wheel, a fourth synchronous belt, a driven synchronous wheel, an upper computer and a second driving synchronous wheel;

[0060] One end of the third synchronous belt is connected with the conveying motor, the other end of the third synchronous belt is sleeved on the first driving synchronous wheel, one end of the fourth synchronous belt is sleeved on the second driving synchronous wheel, the other end of the fourth synchronous belt is sleeved on the driven synchronous wheel, and one side of the fourth synchronous belt is connected with the second transmission carrier;

[0061] The first driving synchronous wheel and the second driving synchronous wheel are respectively mounted at both ends of the connecting shaft, and the connecting shaft is rotatably mounted on the support;

[0062] When the transmission motor rotates, the third synchronous belt can drive the first driving sprocket to rotate, and then the second driving sprocket can rotate synchronously and drive the other end of the fourth synchronous belt to move around the driven sprocket, at this time the second transmission carrier moves with the fourth synchronous belt, wherein the transmission motor is provided with an encoder, and the encoder is electrically connected with the upper computer.

[0063] Preferably, the middle part of the support plate is provided with a rectangular hole in the length direction, and the upper end of the second transmission carrier is connected with the conveying hook through the rectangular hole and can drive the conveying hook to move in the direction of the rectangular hole.

[0064] The conveying hook is in an I-shaped structure; the left and right sides of the conveying hook form a first accommodating space and a second accommodating space, respectively, and when the two reagent cards are placed in the first accommodating space and the second accommodating space, respectively, the conveying hook can push the reagent cards to move between the discard position and the incubation position when the conveying hook moves.

[0065] The discard position is provided with two discard through holes, and the two discard through holes correspond to the positions of the first accommodating space and the second accommodating space in the up-down direction, respectively.

[0066] Preferably, the X-axis sample adding mechanism comprises a sample adding support shell and a sample adding movement assembly.

[0067] The sample adding support shell is provided with a second sliding rail;

[0068] The sample adding movement assembly comprises an X-direction movement support, an X-direction motor, a fourth synchronous belt, an X-direction driving sprocket, and an X-direction driven sprocket.

[0069] The second sliding rail, the X-direction motor, the X-direction driving sprocket, and the X-direction driven sprocket are all mounted on the sample adding support shell.

[0070] The two ends of the fourth synchronous belt are respectively sleeved on the X-direction driving sprocket and the X-direction driven sprocket.

[0071] The X-direction movement support is mounted on the second sliding rail and can slide on the second sliding rail, and the X-direction movement support is connected with the fourth synchronous belt.

[0072] The X-direction motor and the X-direction driving sprocket are respectively mounted on the two sides of the sample adding support shell, and the X-direction motor can drive the X-direction driving sprocket to rotate to drive the fourth synchronous belt to slide the X-direction movement support in the length direction of the second sliding rail.

[0073] Preferably, the sample adding movement assembly comprises a plunger pump, a Z-direction motor, a third driving lead screw, a third sliding rail, and a Z-direction movement support.

[0074] The Z-direction motor, the third slide rail are all installed on the X-direction moving support, the Z-direction moving support is installed on the third slide rail and can slide along the third slide rail;

[0075] One end of the third driving screw rod is connected with the Z-direction motor, the other end of the third driving screw rod is connected with the Z-direction moving support, when the Z-direction motor rotates, the third driving screw rod rotates and drives the Z-direction moving support to move vertically upward or downward along the third slide rail;

[0076] The TIP needle rod is installed on the Z-direction moving support, the plunger pump is connected with the TIP needle rod through a pipeline.

[0077] Preferably, the TIP conveying mechanism comprises a TIP box, a TIP box conveying motor, a TIP box conveying driving wheel, a TIP box conveying driven wheel, a TIP box conveying support table, a TIP box conveying linkage frame and a TIP box synchronous belt;

[0078] The TIP box conveying support table is provided with a TIP box support plate, the TIP box is installed on the TIP box support plate through a TIP box sliding plate, the TIP box conveying linkage frame is installed below the TIP box support plate and extends outward and upward at both ends and is connected with both ends of the TIP box sliding plate respectively;

[0079] The TIP box conveying linkage frame is connected with the TIP box synchronous belt;

[0080] The TIP box conveying motor, the TIP box conveying driving wheel and the TIP box conveying driven wheel are all installed on the TIP box conveying support table, both ends of the TIP box synchronous belt are sleeved on the TIP box conveying driving wheel and the TIP box conveying driven wheel respectively;

[0081] When the TIP box conveying motor rotates, the TIP box conveying driving wheel can be driven to rotate and drive the TIP box synchronous belt to move, and then drive the TIP box conveying linkage frame to drive the TIP box sliding plate and the TIP box to move on the TIP box support plate.

[0082] Compared with the prior art, the present application has the following beneficial effects:

[0083] 1、The present application is designed for sample pretreatment, sample adding, reagent card incubation and reagent card detection, and is integrated into a full-automatic device, so that doctors only need to put samples and reagent cards into the device, and subsequent processes are automatically performed, thereby greatly reducing operation errors and the workload of doctors, improving the detection accuracy and greatly improving the detection efficiency.

[0084] 2、The TIP conveying mechanism in the application can automatically convey the TIP box to a specified position, facilitate the X-axis sample adding mechanism to take the TIP head, and improve the automation level of the device.

[0085] 3、The incubation mechanism in the application can automatically grab the reagent card into a sample adding position to drop the sample, then enter an incubation bin to incubate, after the incubation is completed, enter the detection mechanism to take a photo to check the result, and finally discard the reagent card that has completed the detection, which greatly improves the detection efficiency.

[0086] 4、The X-axis sample adding mechanism in the application can realize X-direction horizontal movement and Z-direction vertical up-and-down movement to realize the action of installing and sucking the sample of the TIP head, then drop the sample solution into the detection reagent card for incubation, and finally discard the TIP head, which is clever in structure design, coherent in action, and improves the operation efficiency.

[0087] 5、The mixing operation in the application is realized by the mixing mechanism to ensure the accuracy and consistency of the sample pretreatment, the diluent is added by using the peristaltic pump in cooperation with the stepping motor to improve the liquid adding precision, improve the mixing quality and precision, meet the actual requirements, and has strong practicality and great market promotion prospect.

[0088] 6、The mixing in the application adopts extrusion mixing, the sample tube passes through two narrow zones to produce deformation, the cotton swab is extruded by the deformed tube wall to achieve the similar function of hand kneading, since the extrusion position is fixed and the extrusion equipment is unchanged, the extrusion effect is consistent every time, and the mixing quality is greatly improved.

[0089] 7、The application realizes continuous mixing operation by designing the first feeding position, liquid adding extrusion position and discharging position in combination with multiple motors, which not only ensures the mixing quality, but also improves the efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0090] Other characteristics, objects and advantages of the application will become more apparent through reading the following detailed description of non-limiting embodiments, made with reference to the accompanying drawings:

[0091] Figure 1 is a structural schematic view of the application;

[0092] Figure 2 is a structural schematic view of the X-axis sample adding mechanism;

[0093] Figure 3 is a structural schematic view of the TIP conveying mechanism;

[0094] Figure 4 is a structural schematic view of the feeding mechanism;

[0095] Figure 5 is a structural schematic view of the sample rack;

[0096] Figure 6 Structure diagram of the first driving device;

[0097] Figure 7 Structure diagram of the second driving device;

[0098] Figure 8 Structure diagram of the liquid adding device;

[0099] Figure 9 Structure diagram of the extruding device;

[0100] Figure 10 Structure diagram of the discharging device;

[0101] Figure 11 Structure diagram of the incubation mechanism;

[0102] Figure 12 Structure diagram of the discarding position and the feeding position;

[0103] Figure 13 Structure diagram of the incubation bin;

[0104] Figure 14 Structure diagram of the reagent card conveying mechanism.

[0105] Shown in the figure are:

[0106] A carrier frame 1;

[0107] A carrier plate 2;

[0108] A sample rack storage area 3;

[0109] A sample rack 4;

[0110] A first transmission carrier 5;

[0111] A first motor 6;

[0112] A second motor 7;

[0113] A first driving lead screw 8;

[0114] A first bearing seat 9;

[0115] A second bearing seat 10;

[0116] A first support leg 11;

[0117] A second support leg 12;

[0118] A protective plate 13;

[0119] A first pulley 14;

[0120] A first synchronous belt 15;

[0121] A second pulley 16;

[0122] Transmission member 17;

[0123] Drive push plate 18;

[0124] Liquid adding motor 19;

[0125] First through hole 20;

[0126] Second through hole 21;

[0127] Clamping head 22;

[0128] Fixed plate 23;

[0129] Sample adding needle 24;

[0130] Discharge motor 25;

[0131] Discharge push rod 26;

[0132] Second drive lead screw 27;

[0133] Discharge base 28;

[0134] Discharge guide rod 29;

[0135] Sample clamping arm 30;

[0136] Sample tube 31;

[0137] Sample rack base 32;

[0138] Sample tube carrier 33;

[0139] First feeding position 34;

[0140] Liquid adding extrusion position 35;

[0141] Discharge position 36;

[0142] Carrying table 41;

[0143] Discarding position 42;

[0144] Second feeding position 43;

[0145] Detection position 44;

[0146] Sample adding position 45;

[0147] Incubation position 46;

[0148] Conveying hook 47;

[0149] Lifting plate 48;

[0150] First power assembly 49;

[0151] Supporting plate 50;

[0152] reagent card 51;

[0153] conveying motor 52;

[0154] third synchronous belt 53;

[0155] first driving sprocket 54;

[0156] fourth synchronous belt 55;

[0157] driven sprocket 56;

[0158] conveying guide rod 57;

[0159] second transmission carrier 58;

[0160] support 59;

[0161] incubation bin 60;

[0162] second driving sprocket 61;

[0163] third support leg 62;

[0164] fourth support leg 63;

[0165] first limiting plate 64;

[0166] second limiting plate 65;

[0167] darkroom 66;

[0168] camera 67;

[0169] incubation bin shell 68;

[0170] incubation motor 69;

[0171] incubation bin 70;

[0172] incubation driving rod 71;

[0173] TIP head 72;

[0174] TIP needle rod 73;

[0175] sample adding support shell 74;

[0176] plunger pump 75;

[0177] second sliding rail 76;

[0178] Z-direction motor 77;

[0179] X-direction movement support 78;

[0180] third driving lead screw 79;

[0181] third sliding rail 80;

[0182] X-direction motor 81;

[0183] Fifth synchronous belt 82;

[0184] X-direction driving wheel 83;

[0185] X-direction driven wheel 84;

[0186] Z-direction moving support 85;

[0187] TIP box 86;

[0188] TIP box conveying motor 87;

[0189] TIP box conveying driving wheel 88;

[0190] TIP box conveying driven wheel 89;

[0191] TIP box conveying support table 90;

[0192] TIP box conveying linkage frame 91;

[0193] TIP box synchronous belt 93;

[0194] TIP box sliding plate 92;

[0195] Feeding mechanism 100;

[0196] X-axis sample adding mechanism 200;

[0197] TIP conveying mechanism 300;

[0198] Incubation mechanism 400;

[0199] Detector mounting table 500. DETAILED DESCRIPTION

[0200] The present application will be described in detail below with specific examples. The following examples will help those skilled in the art to further understand the present application, but do not limit the present application in any form. It should be noted that those skilled in the art can make several changes and improvements without departing from the concept of the present application. These are within the scope of the present application.

[0201] The present application provides a multifunctional full-automatic colloidal gold detector, which comprises a main body, a feeding mechanism, an X-axis sample adding mechanism, a TIP conveying mechanism, an incubation mechanism and a detector mounting table. Figure 1As shown, it comprises a feeding mechanism 100, an X-axis sample adding mechanism 200, a TIP conveying mechanism 300, an incubation mechanism 400, a detector mounting table 500 and a control device, the feeding mechanism 100, the X-axis sample adding mechanism 200 and the TIP conveying mechanism 300 are all mounted on the detector mounting table 500, the feeding mechanism 100 is mounted with a sample rack 4, the sample rack 4 is mounted with a sample tube 31, under the control of the control device, the feeding mechanism 100 can add diluent into the sample tube 31 and mix to form a sample to be used, the incubation mechanism 400 comprises an incubation bin 60 and the incubation mechanism 400 is placed with a reagent card 51, the TIP conveying mechanism 300 is provided with a TIP head 72; the X-axis sample adding mechanism 200 is provided with a TIP needle rod 73, under the control of the control device, the X-axis sample adding mechanism 200 can install the TIP head 72 on the TIP needle rod 73 and can take out the sample from the sample tube 31 through the TIP head 72 and drop it on the reagent card 51, after the reagent card 51 to which the sample is dropped is transported to the incubation bin 60 and incubation is completed, the detection in the incubation mechanism 400 is completed.

[0202] Specifically, as Figure 1 、 Figure 4As shown, the feeding mechanism 100 comprises a carrier frame 1, a first driving device, a second driving device, a liquid adding device, an extruding device and a discharging device, the carrier frame 1 is provided with a carrier plate 2, the carrier plate 2 is sequentially provided with a first feeding position 34, a liquid adding and extruding position 35 and a discharging position 36 along the length direction, the first driving device and the discharging device are respectively arranged on the first feeding position 34 and the discharging position 36, the liquid adding device and the extruding device are respectively arranged on the liquid adding and extruding position 35, the second driving device is arranged on the carrier frame 1 and is provided with a first transmission carrier 5 and a second motor 7, a first through hole 20 is arranged in the middle of the carrier plate 2 along the length direction of the carrier plate 2, the upper end of the first transmission carrier 5 is provided with a carrying space and is arranged on the carrier plate 2, the lower end of the first transmission carrier 5 extends to the lower side of the carrier plate 2 through the first through hole 20 and is drivingly connected with the second motor 7, wherein a sample rack 4 can be matched and arranged in the carrying space, and a sample tube 31 is arranged on the sample rack 4; the sample rack 4 arranged in the sample rack storage area 3 can enter the carrying space under the driving of the first driving device; under the driving of the second motor 7, the first transmission carrier 5 can drive the sample rack 4 to move from the first feeding position 34 to the discharging position 36 through the liquid adding and extruding position 35, and the sample rack 4 moved to the discharging position 36 can leave the discharging position 36 under the driving of the discharging device. In the present application, the liquid adding device can replace the work of the doctor adding diluent, and the extruding device replaces manual mixing, wherein only the feeding and discharging parts are manually added and taken out of the machine by the doctor, thereby greatly improving the work efficiency.

[0203] Further, as shown in Figure 1 、 Figure 6 In actual application, a plurality of sample racks 4 can be placed side by side in the sample rack storage area 3 at a time, and the driving push plate 18 can move a distance of only the width of one sample rack 4 under the driving of the first motor 6 when the mixing mechanism is in operation, so that one sample rack 4 is pushed into the carrying space at a time, thereby improving the efficiency of mixing operation.

[0204] Specifically, as shown in Figure 1 、 Figure 4 、 Figure 7 The carrier frame 1 comprises a first supporting leg 11 and a second supporting leg 12, the first supporting leg 11 and the second supporting leg 12 are respectively arranged at the two ends of the carrier plate 2, the first supporting leg 11 and the second supporting leg 12 play a supporting role, and the area surrounded by the first supporting leg 11, the second supporting leg 12 and the carrier plate 2 provides a placing space for the second driving device.

[0205] Further, as shown in Figure 1 、 Figure 4 、 Figure 7As shown, the second driving device further comprises a first driving screw 8, a first bearing seat 9 and a second bearing seat 10; the first bearing seat 9 and the second bearing seat 10 are respectively installed at two ends of the bottom of the bearing plate 2, the lower end of the first transmission carrier 5 is provided with an internal thread hole, the first driving screw 8 is provided with an external thread matched with the internal thread hole, one end of the first driving screw 8 is installed on the first bearing seat 9, the other end of the first driving screw 8 sequentially passes through the internal thread hole, the second bearing seat 10 and is drivingly connected with the second motor 7, the external thread of the first driving screw 8 is arranged at the middle part, and the positions of the two bearing seats are respectively in interference fit with the inner rings of the bearings.

[0206] Specifically, in one preferred example, as shown in Figure 7 , the second motor 7 is drivingly connected with the first driving screw 8 by using a synchronous belt transmission, for example, by using a second synchronous belt and a matching belt wheel to achieve transmission; in one variant, the second motor 7 is drivingly connected with the first driving screw 8 by using a shaft coupling, for example, the second motor 7 is connected with a matching speed reducer and then connected with the first driving screw 8 through a shaft coupling to achieve driving; in another variant, the second motor 7 is drivingly connected with the first driving screw 8 by using a gear transmission, for example, the second motor 7 is connected with a gear set, the first driving screw 8 is provided with a gear matched with the gear set for meshing, and the transmission is achieved by the connection mode of the gear transmission.

[0207] Specifically, as shown in Figure 1 , Figure 4 , Figure 6 , the first driving device comprises a first motor 6, a first belt wheel 14, a first synchronous belt 15, a second belt wheel 16, a transmission member 17 and a driving push plate 18; the driving push plate 18 is installed on the upper surface of the sample holder storage area 3, the second belt wheel 16 is installed on the side surface of the sample holder storage area 3, and the transmission member 17 is installed on the first synchronous belt 15 and connected with the driving push plate 18; one end of the first synchronous belt 15 is sleeved on the second belt wheel 16, the other end of the first synchronous belt 15 is sleeved on the first belt wheel 14, and the first belt wheel 14 is drivingly connected with the first motor 6; when the first motor 6 rotates, the first belt wheel 14 can be driven to rotate, since the first synchronous belt 15 is in a tight state between the first belt wheel 14 and the second belt wheel 16, the first belt wheel 14 drives the first synchronous belt 15 to move in an elongated circular track, thereby driving the transmission member 17 to drive the driving push plate 18 to push the sample holder 4 placed on the sample holder storage area 3 into the bearing space.

[0208] Specifically, as shown in Figure 1 , Figure 4 , Figure 6As shown, the first driving device further comprises a protection plate 13, wherein the first motor 6 and the first pulley 14 are respectively installed on two sides of the protection plate 13, the transmission member 17 and the driving push plate 18 are respectively installed on two sides of the protection plate 13, the protection plate 13 is provided with a second through hole 21, and the transmission member 17 is connected with the driving push plate 18 penetrating through the second through hole 21.

[0209] Specifically, as shown in Figure 1 , Figure 4 , Figure 8 shown, the liquid adding device comprises a liquid adding motor 19 and a sample adding assembly, the sample adding assembly comprises a sample clamping arm 30, a peristaltic pump, a stepping motor and a sample adding needle 24, the peristaltic pump is drivingly connected with the stepping motor, the liquid adding motor 19 can drive the sample clamping arm 30 to rotate along the axial direction of the liquid adding motor 19, the sample adding needle 24 is clamped by the sample clamping arm 30, when the sample rack 4 moves to a liquid adding extrusion position 35, the sample clamping arm 30 clamping the sample adding needle 24 can move to the upper side of the sample rack 4 under the drive of the liquid adding motor 19 and complete the liquid adding to the sample tube 31 installed on the bearing rack 1 through the peristaltic pump connecting the sample adding needle 24. The diluent of the present application adopts the peristaltic pump, the liquid adding amount is accurately controlled by the number of turns of the pump driven by the stepping motor. Since the peristaltic pump has the characteristic that the liquid amount per turn is constant, the control of the number of turns can control the liquid adding precision to meet the requirements

[0210] Specifically, as shown in Figure 1 , Figure 4 , Figure 9 shown, the extrusion device comprises a clamping head 22, a fixed plate 23 and a spring; the clamping head 22 and the fixed plate 23 are both installed on the liquid adding extrusion position 35; the number of the clamping head 22 is four and adopts a flexible structure, wherein two clamping heads 22 are installed on one side of the first through hole 20, the other two clamping heads 22 are installed on the other side of the first through hole 20, the other two clamping heads 22 are connected with the fixed plate 23 and the inside of the other two clamping heads 22 are both installed with springs; the clamping heads 22 on both sides correspond to each other and a narrow channel is formed between each two corresponding clamping heads 22, the width of the narrow channel is smaller than the diameter of the sample tube 31 and the width of the narrow channel can be adjusted according to the actual situation. Since the outer wall of the sample tube 31 is relatively soft, when the sample tube passes through the narrow channel, the sample tube 31 will be extruded and deformed, the cotton swab in the sample tube will be extruded by the wall of the sample tube 31, achieving the effect of hand squeezing. The spring in one side of the clamping head 22 can make the clamping head have appropriate elasticity, ensuring that the sample rack 4 can smoothly pass through the narrow channel.

[0211] Specifically, as shown in Figure 1 , Figure 4 , Figure 10As shown, the discharging device comprises a discharging motor 25, a discharging push rod 26, a second driving lead screw 27, a discharging base 28 and two discharging guide rods 29. The discharging base 28 is installed on the discharging position 36. The discharging motor 25 is installed on the discharging base 28 and two discharging through holes are respectively arranged on the discharging base 28 and on both sides of the discharging motor 25. The two discharging guide rods 29 are installed on the discharging push rod 26 and can slide in the two discharging through holes. One end of the second driving lead screw 27 is installed on the discharging push rod 26 and the other end of the second driving lead screw 27 is connected with the discharging motor 25. When the discharging motor 25 operates, the second driving lead screw 27 can drive the discharging push rod 26 to move along the length direction of the second driving lead screw 27, and the two discharging guide rods 29 can slide in the two discharging through holes.

[0212] Specifically, as shown in Figure 1 、 Figure 4 、 Figure 5 The sample rack 4 comprises a sample rack base 32 and a sample tube carrier 33. The sample tube carrier 33 is connected with the sample rack base 32 through a plurality of connecting ribs. A plurality of concave installation grooves are arranged on the sample rack base 32 in sequence and equidistantly. A plurality of cylindrical placement spaces are arranged on the sample tube carrier 33 in sequence and equidistantly. The placement spaces and the installation grooves are one-to-one corresponding. The sample tube 31 is installed into the installation groove through the placement space. One end of the connecting rib is arranged between two adjacent installation grooves. The other end of the connecting rib is arranged between two adjacent placement spaces. The width of the connecting rib is smaller than the diameter of the sample tube 31, so that the clamping heads 22 on both sides can be pressed to the two sides of the sample tube 31 without being blocked by the connecting rib.

[0213] Specifically, as shown in Figure 1 、 Figure 11As shown, the incubation mechanism 400 comprises a reagent card conveying mechanism, a support plate 41, and an incubation bin 60. The support plate 41 is provided with a support plate 50, which comprises, in sequence from the direction close to the incubation bin 60, a discarding position 42, a second feeding position 43, a detection position 44, a sample adding position 45, and an incubation position 46, wherein the incubation position 46 extends into the interior of the incubation bin 60. The reagent card conveying mechanism comprises a conveying hook 47, which is installed on the support plate 50 and can move between the discarding position 42 and the incubation position 46 under the driving of the reagent card conveying mechanism. According to the detection order of the reagent card 51, the second feeding position 43 takes out the reagent card 51 into the sample adding position 45, and after the detection sample is added dropwise on the sample adding position 45, the reagent card 51 enters the incubation position 46, and after incubation at a rated temperature for 10 minutes, the reagent card 51 enters the detection position 44 for photographic detection, and finally enters the discarding position 42 to discard the sample.

[0214] Further, as shown in Figure 1 、 Figure 11 、 Figure 12 As shown, the second feeding position 43 is provided with a lifting plate 48 and a first power assembly 49. The reagent card 51 is placed on the lifting plate 48 by a first mechanical arm. The number of the lifting plate 48 is two, and the two lifting plates 48 are placed on the two sides of the conveying hook 47. The lifting plate 48 can move between a first position and a second position under the driving of the first power assembly 49. When the lifting plate 48 moves the reagent card 51 to the first position, the conveying hook 47 moves to the second feeding position 43 under the driving of the reagent card conveying mechanism. At this time, the lifting plate 48 moves to the second position under the driving of the first power assembly 49. The reagent card 51 enters the working range of the conveying hook 47. The conveying hook 47 can drive the reagent card 51 to pass through the detection position 44, the sample adding position 45, and enter the incubation position 46 in sequence. In a preferred example, the first power assembly 49 is driven by a motor, and the conveying hook 47 is in the shape of a I-shaped structure. The left and right sides of the conveying hook 47 form a first accommodating space and a second accommodating space, respectively. When the two reagent cards 51 are placed in the first accommodating space and the second accommodating space, respectively, the conveying hook 47 can push the reagent card 51 to move between the discarding position 42 and the incubation position 46. In a variant, the first power assembly 49 is driven by a pneumatic cylinder.

[0215] Specifically, as shown in Figure 1 、 Figure 11 、 Figure 12 The lifting of the reagent card 51 is realized by a lifting mechanism mainly composed of a lifting motor and a lifting plate 48 in the second feeding position 43. The main function is to first lift the reagent card 51, so that the conveying hook 47 enters Figure 12The reagent card 51 is then lowered on both sides of the conveying hook 47, so that the conveying hook 47 can take the reagent card 51 from the second feeding position 43 to the sample adding position 45. The upper lifting plate 48 is raised to lift the remaining reagent card 51 to the first position, which can leave a passage below the upper lifting plate 48 to facilitate the conveying hook 47 to pass through the upper lifting plate 48 from below to enter the discarding position 42 for discarding. Alternatively, the conveying hook 47 can enter the incubation position 46 for the next reagent card 51 taking work. It should be noted that the cooperation between the conveying hook 47 and the upper lifting plate 48 must follow the process to avoid collision. In a preferred embodiment, a detection device is further included, which can detect the movement position of the upper lifting plate 48 and transmit the detection information to the upper computer to control the movement of the conveying motor 52 and avoid collision between the conveying hook 47 and the upper lifting plate 48. Figure 1

[0216] Specifically, as shown in Figure 1 、 Figure 11 、 Figure 13 The incubation bin 60 is internally provided with an incubation bin 70, which includes two incubation sub-bins and the two incubation sub-bins are respectively arranged on both sides of the incubation bin 70 along the width direction of the support plate 50. The incubation sub-bins include a plurality of incubation layer positions, which are equidistantly arranged in the incubation sub-bins in the up-down direction. The conveying hook 47 entering the incubation position 46 can further drive the reagent card 51 incubated on another incubation layer position to move to the detection position 44 for detection, and then finally transport the detected reagent card 51 to the discarding position 42 for discarding.

[0217] Further, as shown in Figure 13 The incubation bin 60 includes an incubation bin shell 68 and an incubation motor 69. The incubation bin 70 is installed inside the incubation bin shell 68. The inner wall of the incubation bin shell 68 is provided with a first sliding rail in the height direction. The incubation bin 70 is provided with a sliding block matched and installed on the first sliding rail. The incubation motor 69 is installed above the incubation bin shell 68 and is provided with an incubation driving rod 71 extending through the top of the incubation bin shell 68 into the interior of the incubation bin shell 68 and connected with the incubation bin 70. The incubation position 46 is provided with an incubation position through hole. When the incubation motor 69 operates, the incubation driving rod 71 can be driven to move upward or downward, thereby driving the incubation bin 70 to move upward or downward through the incubation position through hole under the guidance of the first sliding rail.

[0218] Further, as shown in Figure 1 、 Figure 11 、 Figure 13 ​As shown, the incubation site 46 is mainly driven by the incubation motor 69 to lift and lower the incubation material bin 60, and after the reagent card 51 is delivered to the sample adding site 45 by the delivery hook 47, the reagent card 51 is directly delivered to the incubation material bin 60 by the delivery hook 47, and then the two incubation sub-bins on the incubation material bin 60 are lowered by one level synchronously, that is, the incubation layer site drives the reagent card 51 after sample adding to be lowered by one level, at this time, there are two cases, in one case, the upper incubation layer site stores the reagent card 51 which has been incubated, at this time, the reagent card 51 falls on both sides of the delivery hook 47, and when the delivery hook 47 is moved out of the incubation material bin 60, the incubated reagent card 51 is taken out and then moved to the detection site 44 to be photographed by the camera, and then moved to the discarding site 42 to discard the reagent card 51. In another case, the upper incubation layer site is empty, that is, there is no reagent card 51 stored, then the delivery hook 47 moves back to the second feeding site 43 to take the next reagent card 51, and then the sample adding site 45 adds sample and then delivers to the incubation layer site for incubation operation, and so on, to ensure the continuity of the incubation operation.

[0219] The incubation bin 70 of the present application adopts a lifting structure to ensure that the whole incubation bin is kept at a constant temperature at a rated temperature, and adopts a symmetrical structure to place one incubation sub-bin on the left and one on the right, which can increase the throughput of detection. While the first reagent card 51 after sample adding is directly moved to the incubation sub-bin to wait for 10 minutes for detection, the reagent card delivery mechanism can continue to take out the next reagent card 51 from the feeding site 2 to add sample and then move to the incubation bin 70. In a preferred example, 15 incubation layer sites are designed in one incubation sub-bin, which can ensure that 15 samples are sequentially moved in for incubation, and the work is carried out in a pipeline manner, that is, one in and one out to achieve continuous detection.

[0220] Specifically, as shown in Figure 1 、 Figure 11 、 Figure 13 、 Figure 14 The reagent card delivery mechanism includes a delivery guide rod 57, a second transmission carrier 58 and a second power assembly, the object table 41 further includes a third support leg 62 and a fourth support leg 63, the third support leg 62 and the fourth support leg 63 are respectively installed at both ends of the lifting plate 48, the two ends of the delivery guide rod 57 are respectively installed on the third support leg 62 and the fourth support leg 63 and are arranged below the lifting plate 48, the second transmission carrier 58 is provided with a transmission carrier through hole, the lower end of the second transmission carrier 58 is sleeved on the delivery guide rod 57 through the transmission carrier through hole and can slide relative to the delivery guide rod 57, the upper end of the second transmission carrier 58 is connected with the delivery hook 47, and the second power assembly can drive the second transmission carrier 58 to slide along the direction of the delivery guide rod 57 and simultaneously drive the delivery hook 47 to slide above or below the lifting plate 48.

[0221] Further, as shown in Figure 1 、 Figure 11 、 Figure 13 、 Figure 14 , the second power assembly comprises a transmission motor 52, a third synchronous belt 53, a first driving synchronous wheel 54, a fourth synchronous belt 55, a driven synchronous wheel 56, an upper computer and a second driving synchronous wheel 61; one end of the third synchronous belt 53 is connected with the transmission motor 52, the other end of the third synchronous belt 53 is sleeved on the first driving synchronous wheel 54, one end of the fourth synchronous belt 55 is sleeved on the second driving synchronous wheel 61, the other end of the fourth synchronous belt 55 is sleeved on the driven synchronous wheel 56 and one side of the fourth synchronous belt 55 is connected with a second transmission carrier 58; the first driving synchronous wheel 54 and the second driving synchronous wheel 61 are respectively installed on two ends of a connecting shaft and the connecting shaft is rotatably installed on a support 59; when the transmission motor 52 rotates, the third synchronous belt 53 can drive the first driving synchronous wheel 54 to rotate, and in turn the second driving synchronous wheel 61 can be synchronously rotated and drive the other end of the fourth synchronous belt 55 to move around the driven synchronous wheel 56, at this time the second transmission carrier 58 moves with the fourth synchronous belt 55.

[0222] Specifically, the transmission motor 52 is provided with an encoder, and the encoder is electrically connected with the upper computer. The reagent card conveying mechanism mainly comprises a synchronous wheel driven by the transmission motor 52, and a synchronous belt moved by the synchronous wheel. The conveying hook 47 is connected with the synchronous belt, so that the conveying hook 47 can be moved to any position with the rotation of the motor. It should be noted that the transmission motor 52 is a closed-loop stepping motor with an encoder, and the position of the conveying hook 47 can be obtained by the encoder and transmitted to the upper computer and recorded in the database. In the case of power failure or equipment failure caused by various unknown factors, the position of the conveying hook 47 can be obtained from the database, which facilitates the logical relationship between the second feeding position 43, the incubation position 46 and the conveying hook 47 in subsequent operation, and avoids errors.

[0223] It should be pointed out that the cooperation between the reagent card conveying mechanism and the incubation bin lifting mechanism, and the cooperation between the reagent card conveying mechanism and the second feeding position 43 lifting mechanism are the keys. Since the reagent card conveying mechanism transports the reagent card 51 in the horizontal direction, and the incubation bin 70 and the second feeding position 43 transport the reagent card 51 in the vertical direction, improper control at the intersection can easily cause card plate, impact and other phenomena. Therefore, the incubation motor 69 is also connected with the upper computer signal, and the upper computer performs logical judgment on the obtained position of the conveying hook 47 and forms a control relationship with the incubation motor 69, so as to ensure the reliability of movement connection. At the same time, the program can feedback where the fault occurs in time when the accident occurs.

[0224] Specifically, as shown in Figure 12As shown, the middle part of the support plate 50 is provided with a rectangular hole along the length direction, and the upper end of the second transmission carrier 58 passes through the rectangular hole and is connected with the transmission hook 47 and can drive the transmission hook 47 to move along the direction of the rectangular hole.

[0225] Specifically, as shown in the figure, Figure 12 The discard position 42 is provided with two discard through holes, which correspond to the positions of the first and second accommodation spaces in the vertical direction. When the transmission hook 47 drives the tested reagent card 51 to move to the position of the discard through hole, the reagent card 51 falls from the discard through hole and is discarded and recycled.

[0226] Specifically, as shown in the figure, Figure 11 , Figure 12 The two sides of the second feeding position 43 along the length direction of the support plate 50 are respectively provided with a first limiting plate 64 and a second limiting plate 65, which are respectively installed on the support plate 50 and are respectively provided with a first gap and a second gap between the support plate 50. The first gap and the second gap are the passages of the transmission hook 47 when moving on the support plate 50. The second feeding position 43 is provided with a first lifting hole and a second lifting hole, and the first lifting hole and the second lifting hole are respectively arranged on the two sides of the rectangular hole. The number of the lifting plates 48 is two, and the two lifting plates 48 can synchronously move between the first position and the second position through the first lifting hole and the second lifting hole, and can move in the space between the first limiting plate 64 and the second limiting plate 65 when the two lifting plates 48 move above the support plate 50. The first limiting plate 64 and the second limiting plate 65 play a limiting and guiding role for the movement of the lifting plate 48, and are also beneficial to the stable movement of the lifting plate 48.

[0227] Specifically, as shown in the figure, Figure 11 , Figure 13 The detection position 44 is provided with a darkroom 66 and a camera 67. The camera 67 is installed on the support plate 50 through the darkroom 66. The darkroom 66 is installed on the support plate 50 and is provided with a third gap between the support plate 50. The third gap is the passage of the transmission hook 47 when moving on the support plate 50. The darkroom 66 is provided with a shooting through hole, and the camera 67 can shoot the reagent card 51 placed in the darkroom 66 of the detection position 44 through the shooting through hole.

[0228] Specifically, as shown in the figure, Figure 1 , Figure 2As shown, the X-axis sample loading mechanism 200 includes a sample loading support housing 74, a sample loading motion assembly, and a plunger pump 75; a second slide rail 76 is provided on the sample loading support housing 74; the sample loading motion assembly includes an X-axis motion bracket 78, an X-axis motor 81, a fifth synchronous belt 82, an X-axis drive wheel 83, and an X-axis driven wheel 84; the second slide rail 76, the X-axis motor 81, the X-axis drive wheel 83, and the X-axis driven wheel 84 are all mounted on the sample loading support housing 74; the fifth synchronous belt 82... The two ends are respectively fitted onto the X-direction driving wheel 83 and the X-direction driven wheel 84; the X-direction motion bracket 78 is mounted on the second slide rail 76 and can slide on the second slide rail 76, and the X-direction motion bracket 78 is connected to the fifth synchronous belt 82; the X-direction motor 81 and the X-direction driving wheel 83 are respectively mounted on both sides of the sample feeding support housing 74, and the X-direction motor 81 can drive the X-direction driving wheel 83 to rotate, thereby driving the fifth synchronous belt 82 to drive the X-direction motion bracket 78 to slide along the length of the second slide rail 76.

[0229] Specifically, such as Figure 2 As shown, the sample loading motion assembly includes a Z-axis motor 77, a third drive screw 79, a third slide rail 80, and a Z-axis motion support 85. The Z-axis motor 77 and the third slide rail 80 are both mounted on the X-axis motion support 78. The Z-axis motion support 85 is mounted on the third slide rail 80 and can slide along the third slide rail 80. One end of the third drive screw 79 is connected to the Z-axis motor 77, and the other end of the third drive screw 79 is connected to the Z-axis motion support 85. When the Z-axis motor 77 rotates, the third drive screw 79 rotates, thereby driving the Z-axis motion support 85 to move vertically upward or downward along the third slide rail 80. The TIP needle rod 73 is mounted on the Z-axis motion support 85, and the plunger pump 75 is connected to the TIP needle rod 73 through a pipeline.

[0230] Specifically, such as Figure 1 , Figure 3As shown, the TIP conveying mechanism 300 comprises a TIP box 86, a TIP box conveying motor 87, a TIP box conveying driving wheel 88, a TIP box conveying driven wheel 89, a TIP box conveying support table 90, a TIP box conveying linkage frame 91 and a TIP box synchronous belt 93; the TIP box conveying support table 90 is provided with a TIP box support plate, the TIP box 86 is installed on the TIP box support plate through a TIP box slide plate 92, the TIP box conveying linkage frame 91 is installed below the TIP box support plate and extends outward and upward at both ends and is connected to both ends of the TIP box slide plate 92 respectively; the TIP box conveying linkage frame 91 is connected with the TIP box synchronous belt 93; the TIP box conveying motor 87, the TIP box conveying driving wheel 88 and the TIP box conveying driven wheel 89 are all installed on the TIP box conveying support table 90, both ends of the TIP box synchronous belt 93 are sleeved on the TIP box conveying driving wheel 88 and the TIP box conveying driven wheel 89 respectively; when the TIP box conveying motor 87 rotates, it can drive the TIP box conveying driving wheel 88 to rotate and drive the TIP box synchronous belt 93 to move, thereby driving the TIP box conveying linkage frame 91 to drive the connected TIP box slide plate 92 and TIP box 86 to move on the TIP box support plate.

[0231] The working principle of the present invention is as follows:

[0232] Firstly, a plurality of sample racks 4 are placed into the sample rack storage area 3, the control device controls the first motor 6 to start to drive the driving push plate 18 to push the sample rack 4 closest to the first through hole 20 to the bearing space on the first transmission carrier 5 at the first feeding position 34, at this time, the control device controls the second motor 7 to start, the second motor 7 drives the first driving lead screw 8 to rotate to make the first transmission carrier 5 move to the liquid adding and extruding position 35; the control device controls the liquid adding motor 19 to start and drive the sample clamping arm 30 to rotate along the axial direction of the liquid adding motor 19, so that the sample adding needle 24 installed on the sample clamping arm 30 moves directly above the mouth of the sample tube 31, at this time, the peristaltic pump connected with the sample adding needle 24 adds diluent to the sample tube 31; after the sample tube 31 is filled with diluent, the control device controls the second motor 7 to drive the first transmission carrier 5 to continue to move and pass through the narrow passage formed by the two sets of clamping heads 22, so that the sample in the sample tube 31 is extruded and mixed uniformly, and then moves to the discharge position 36, and the second motor 7 stops rotating; the control device controls the discharge motor 25 to start and drive the second driving lead screw 27 to move along the length direction of the second driving lead screw 27, so that the sample rack is pushed to the sample discharging position.

[0233] Secondly, the control device controls the TIP box conveying motor 87 to rotate, thereby driving the TIP box conveying driving wheel 88 to rotate and driving the TIP box synchronous belt 93 to move, the TIP box synchronous belt 93 being connected with the TIP box conveying linkage frame 91, thereby driving the TIP box conveying linkage frame 91 to drive the TIP box sliding plate 92 and the TIP box 86 to move on the TIP box support plate, and when moving to the lower side of the Z-direction movement support 85, the control device controls the TIP box conveying motor 87 to stop rotating.

[0234] Thirdly, the control device controls the first power assembly 49 to make the reagent card 51 on the second feeding position 43 be lifted, the conveying hook 47 enters the second feeding position 43 from the bottom of the reagent card 51, the reagent card 51 is lowered to make the reagent card 51 enter the working range of the feeding hook 7, and then the reagent card 51 is pushed by the feeding hook 7 to pass through the detection position 44 and enter the sample adding position 45, at this time, the control device controls the X-direction motor 81 to drive the movement support 78 to move until the TIP needle rod 73 moves to the upper side of one of the TIP heads 72 on the TIP box 86, at this time, the control device controls the Z-direction motor 77 to start and drive the TIP needle rod 73 to move close to the TIP head 72 until the TIP head 72 is installed on the TIP needle rod 73, after the Z-direction movement support 85 drives the TIP head 72 to move upward to a certain height, the control device controls the X-direction motor 81 to drive the movement support 78 to move until moving to the upper side of the sample rack 4, at this time, the control device controls the X-direction motor 81 to drive the movement support 78 to move downward until the TIP head 72 extends into the sample tube 31 to suck the sample into the TIP needle rod 73, after the control device controls the Z-direction movement support 85 to drive the TIP head 72 to move upward to a certain height, the control device controls the X-direction motor 81 to drive the movement support 78 to move to the direction close to the sample adding position 45.

[0235] Finally, when the TIP head 72 moves to the upper side of the sample adding position 45, the control device controls the plunger pump 75 to add the sample to the reagent card 51 moving to the sample adding position 45. After the sample adding is completed, the conveying hook 47 continues to convey the reagent card 51 to the first incubation layer position of the incubation bin 60, the incubation bin 60 is lowered, the conveying hook 47 enters the feeding position from the second incubation layer position after being lowered, and then the second reagent card 51 is continuously brought into the sample adding position 45 for sample adding and then into the second incubation layer position of the incubation bin 60, and so on. When the incubation of the first incubation layer position is completed, the conveying hook 47 brings the reagent card 51 out of the incubation bin 60, enters the detection position 44 for photographing and detection, and then directly enters the discard position 42 from the second gap, the first gap and the lifting plate 48 on the two sides of the second feeding position 43 to discard the reagent card 51, and the used TIP head 72 is discarded and a new TIP head 72 is installed for use.

[0236] The application integrates sample pretreatment, automatic sample adding, automatic incubation and automatic detection, replaces manual operation to realize full-process automation and reduces the influence of human factors.

[0237] In the description of the present application, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0238] The specific embodiments of the application are described above. It should be understood that the application is not limited to the specific embodiments described above, and various changes or modifications can be made by those skilled in the art within the scope of the claims, which does not affect the essential content of the application. The embodiments of the present application and the features in the embodiments can be arbitrarily combined without conflict.

Claims

1. A multifunctional fully automatic colloidal gold detector, characterized in that, It includes a feeding mechanism (100), an X-axis sample feeding mechanism (200), a TIP conveying mechanism (300), an incubation mechanism (400), a detector mounting platform (500), and a control device; The feeding mechanism (100), X-axis sample feeding mechanism (200), TIP conveying mechanism (300), and incubation mechanism (400) are all installed on the testing instrument mounting platform (500); The feeding mechanism (100) includes a sample rack (4) on which a sample tube (31) is installed. Under the control of the control device, the feeding mechanism (100) can add diluent to the sample tube (31) and mix it to form a sample ready for use. The incubation device (400) includes a reagent card (51) and an incubation hopper (60); The TIP transmission mechanism (300) is provided with a TIP header (72). The X-axis sample loading mechanism (200) is equipped with a TIP needle bar (73). Under the control of the control device, the X-axis sample loading mechanism (200) can install the TIP head (72) onto the TIP needle bar (73) and can take out the sample from the sample tube (31) through the TIP head (72) and drop it onto the reagent card (51). The reagent card (51) with the sample is then transported to the incubation hopper (60) for incubation. After incubation, the test is completed in the incubation mechanism (400). The feeding mechanism (100) includes a support frame (1) on which a support plate (2) is mounted. The bearing plate (2) includes a liquid filling and extrusion position (35) and a discharge position (36); The feeding mechanism (100) also includes a liquid adding device, a squeezing device and a discharging device; the liquid adding device includes a liquid adding motor (19) and a sample adding assembly, the sample adding assembly is provided with a sample clamping arm (30), a peristaltic pump, a stepper motor and a sample adding needle (24), the peristaltic pump is driven and connected to the stepper motor; When the sample holder (4) moves to the liquid filling and squeezing position (35), the clamping arm (30) holding the sample needle (24) can move above the sample holder (4) under the drive of the liquid filling motor (19) and connect to the sample needle (24) through the peristaltic pump to complete the liquid filling into the sample tube (31) installed on the support frame (1); The extrusion device includes a clamping head (22), a fixing plate (23), and a spring; The clamping head (22) and the fixing plate (23) are both installed on the liquid filling and squeezing position (35); The number of clamping heads (22) is four, of which two clamping heads (22) are installed on one side of the first through hole (20), and the other two clamping heads (22) are installed on the other side of the first through hole (20). The other two clamping heads (22) are connected to the fixing plate (23), and springs are installed inside the other two clamping heads (22). The clamping heads (22) on both sides are paired and a narrow channel is formed between each pair of corresponding clamping heads (22), and the width of the narrow channel is smaller than the diameter of the sample tube (31); The discharge device includes a discharge motor (25), a discharge push rod (26), a second drive screw (27), a discharge base (28), and a discharge guide rod (29). The discharge base (28) is installed on the discharge position (36), the discharge motor (25) is installed on the discharge base (28) and two discharge through holes are respectively provided on the discharge base (28) and respectively located on both sides of the discharge motor (25). One end of the two discharge guide rods (29) is respectively installed on the discharge push rod (26), and the other end of the two discharge guide rods (29) is respectively installed in the two discharge through holes and can slide relative to the discharge through holes. One end of the second drive screw (27) is mounted on the discharge push rod (26), and the other end of the second drive screw (27) is connected to the discharge motor (25). When the discharge motor (25) is running, it can drive the second drive screw (27) to drive the discharge push rod (26) to move along the length of the second drive screw (27). At the same time, the two discharge guide rods (29) follow the discharge push rod (26) and slide in the two discharge through holes. The feeding mechanism (100) includes a second drive device; The second drive device also includes a first drive screw (8); The external thread of the first drive screw (8) is located in the middle part.

2. The multifunctional fully automatic colloidal gold detector according to claim 1, characterized in that, The feeding mechanism (100) includes a first driving device, a liquid adding device, a squeezing device, and a discharging device; The support frame (1) is equipped with a support plate (2), and the support plate (2) includes a first feeding position (34), a liquid addition and extrusion position (35), and a discharge position (36) along the length direction. The first driving device and the discharge device are respectively installed on the first feeding position (34) and the discharge position (36), and the liquid adding device and the extrusion device are respectively installed on the liquid adding and extrusion position (35); The second drive device is mounted on the support frame (1) and the second drive device is provided with a first transmission carrier (5) and a second motor (7). The middle of the support plate (2) is provided with a first through hole (20) along the length direction of the support plate (2). The upper end of the first transmission carrier (5) is provided with a support space and is mounted on the support plate (2). The lower end of the first transmission carrier (5) passes through the first through hole (20) and extends to the bottom of the support plate (2) and is driven and connected to the second motor (7). The sample rack (4) can be matched and installed in the support space. The first feeding position (34) is connected to a sample rack storage area (3), and the sample rack (4) placed in the sample rack storage area (3) can be transported to the carrying space under the drive of the first driving device; Driven by the second motor (7), the first transmission carrier (5) can drive the sample rack (4) from the first feeding position (34) through the liquid extrusion position (35) to the discharge position (36). The sample rack (4) that has moved to the discharge position (36) can be pushed out of the discharge position (36) under the drive of the discharge device. The sample rack (4) includes a sample rack base (32) and a sample tube carrier (33); The sample tube carrier (33) and the sample rack base (32) are connected by multiple connecting ribs; The sample holder base (32) has multiple recessed mounting slots arranged at equal intervals in sequence, and the sample tube carrier (33) has multiple cylindrical placement spaces arranged at equal intervals in sequence. The placement spaces correspond one-to-one with the mounting slots. When the sample tube (31) is placed, it is installed into the mounting slot through the placement space. One end of the connecting rib is set between two adjacent mounting slots, and the other end of the connecting rib is set between two adjacent placement spaces. The width of the connecting rib is smaller than the diameter of the sample tube (31).

3. The multifunctional fully automatic colloidal gold detector according to claim 2, characterized in that, The second drive device also includes a first bearing housing (9) and a second bearing housing (10); The first bearing housing (9) and the second bearing housing (10) are respectively installed at both ends of the bottom of the bearing plate (2). The lower end of the first transmission carrier (5) is provided with an internal threaded hole. The first drive screw (8) is provided with an external thread that matches the internal threaded hole. One end of the first drive screw (8) is installed on the first bearing housing (9). The other end of the first drive screw (8) passes through the internal threaded hole and the second bearing housing (10) in sequence and is driven by the second motor (7). The driving method of the second motor (7) and the first drive screw (8) includes any of the following structural forms: - Synchronous belt drive is used; - Uses a coupling for transmission; - Gear transmission is used; The first drive device includes a first motor (6), a protective plate (13), a first pulley (14), a first synchronous belt (15), a second pulley (16), a transmission component (17), and a drive push plate (18). The drive push plate (18) is installed on the top of the sample rack storage area (3), the second pulley (16) is installed on the side of the sample rack storage area (3), and the transmission component (17) is installed on the first synchronous belt (15) and connected to the drive push plate (18). One end of the first synchronous belt (15) is fitted onto the second pulley (16), and the other end of the first synchronous belt (15) is fitted onto the first pulley (14). The first pulley (14) is driven by the first motor (6). When the first motor (6) rotates, it can drive the first pulley (14) to rotate and drive the first synchronous belt (15) to move, which in turn can drive the transmission component (17) to drive the drive push plate (18) to push the sample rack (4) placed on the sample rack storage area (3) into the bearing space. The first motor (6) and the first pulley (14) are respectively installed on both sides of the protective plate (13). The transmission component (17) and the drive push plate (18) are respectively installed on both sides of the protective plate (13). The protective plate (13) is provided with a second through hole (21). The transmission component (17) is connected to the drive push plate (18) that passes through the second through hole (21).

4. The multifunctional fully automatic colloidal gold detector according to claim 1, characterized in that, The incubation mechanism (400) also includes a reagent card delivery mechanism and a stage (41). The stage (41) is provided with a support plate (50), which includes, in sequence with respect to the incubation hopper (60), a discarding position (42), a second feeding position (43), a detection position (44), a sample adding position (45), and an incubation position (46), wherein the incubation position (46) extends into the interior of the incubation hopper (60); The reagent card delivery mechanism includes a delivery hook (47) which is mounted on a support plate (50) and can move between a discard position (42) and an incubation position (46) under the drive of the reagent card delivery mechanism. The second feed position (43) is provided with a lifting plate (48) and a first power component (49). The reagent card (51) is placed on the lifting plate (48). The lifting plate (48) can move between a first position and a second position under the drive of the first power component (49). The first power component (49) is driven by a motor or a cylinder. When the lifting plate (48) moves the reagent card (51) to the first position, the conveying hook (47) moves to the second feeding position (43) under the drive of the reagent card conveying mechanism. At this time, the lifting plate (48) moves to the second position under the drive of the first power component (49), and the reagent card (51) enters the working range of the conveying hook (47). The conveying hook (47) can drive the reagent card (51) to pass through the detection position (44), the sample addition position (45) in sequence and enter the incubation position (46). The incubation hopper (60) is equipped with an incubation hopper (70). The incubation hopper (70) includes two incubation compartments, which are arranged at intervals along the width of the support plate (50) on both sides inside the incubation hopper (70). The incubation compartments include multiple incubation layers, which are arranged at equal intervals in the vertical direction. The conveying hook (47) that enters the incubation position (46) can move the reagent card (51) that has been incubated on another incubation layer to the detection position (44). After the detection is completed, the reagent card (51) that has been detected is finally transported to the disposal position (42) for disposal. The incubation hopper (60) includes an incubation hopper shell (68) and an incubation motor (69); The incubation chamber (70) is installed inside the incubation chamber shell (68); The inner wall of the incubation chamber shell (68) is provided with a first slide rail along the height direction, and the incubation chamber (70) is provided with a slider, which is matched and installed on the first slide rail; The incubation motor (69) is installed above the incubation chamber shell (68) and an incubation drive rod (71) is provided on the incubation motor (69). The incubation drive rod (71) extends through the top of the incubation chamber housing (68) into the interior of the incubation chamber housing (68) and connects to the incubation chamber (70). The incubation position (46) is provided with an incubation position through hole. When the incubation motor (69) is running, it can drive the incubation drive rod (71) to move upward or downward, thereby driving the incubation chamber (70) to move upward or downward through the incubation position through hole under the guidance of the first slide rail. The detection position (44) is equipped with a darkroom (66) and a camera (67). The camera (67) is mounted on the support plate (50) via a darkroom (66); The dark chamber (66) is installed on the support plate (50) and a third gap is provided between it and the support plate (50); the dark chamber (66) is provided with a shooting through hole, and the camera (67) can shoot the reagent card (51) placed on the detection position (44) through the shooting through hole.

5. The multifunctional fully automatic colloidal gold detector according to claim 4, characterized in that, The reagent card delivery mechanism also includes a delivery guide rod (57), a second transmission carrier (58), and a second power assembly; The platform (41) also includes a third support leg (62) and a fourth support leg (63), which are respectively installed at both ends of the lifting plate (48); The two ends of the transmission guide rod (57) are respectively installed on the third support leg (62) and the fourth support leg (63) and are located below the lifting plate (48); The second transmission carrier (58) is provided with a transmission carrier through hole. The lower end of the second transmission carrier (58) is fitted onto the transmission guide rod (57) through the transmission carrier through hole and can slide relative to the transmission guide rod (57). The upper end of the second transmission carrier (58) is connected to the transmission hook (47). The second power assembly can drive the second transmission carrier (58) to slide along the direction of the transmission guide rod (57) and simultaneously drive the transmission hook (47) to slide on the lifting plate (48); The second power assembly includes a transmission motor (52), a third synchronous belt (53), a first active synchronous pulley (54), a fourth synchronous belt (55), a driven synchronous pulley (56), a host computer, and a second active synchronous pulley (61). One end of the third synchronous belt (53) is connected to the transmission motor (52), and the other end of the third synchronous belt (53) is fitted on the first active synchronous pulley (54). One end of the fourth synchronous belt (55) is fitted on the second active synchronous pulley (61), and the other end of the fourth synchronous belt (55) is fitted on the driven synchronous pulley (56). One side of the fourth synchronous belt (55) is connected to the second transmission carrier (58). The first active synchronizing wheel (54) and the second active synchronizing wheel (61) are respectively installed at both ends of the connecting shaft, and the connecting shaft is rotatably installed on the support (59); When the transmission motor (52) rotates, it can drive the third synchronous belt (53) to drive the first active synchronous pulley (54) to rotate, and then drive the second active synchronous pulley (61) to rotate synchronously and drive the other end of the fourth synchronous belt (55) to move around the driven synchronous pulley (56). At this time, the second transmission carrier (58) follows the fourth synchronous belt (55) to move. The transmission motor (52) is equipped with an encoder, which is electrically connected to the host computer.

6. The multifunctional fully automatic colloidal gold detector according to claim 5, characterized in that, The support plate (50) has a rectangular hole in the middle along the length direction. The upper end of the second transmission carrier (58) passes through the rectangular hole and is connected to the transmission hook (47), and can drive the transmission hook (47) to move along the direction of the rectangular hole. The transfer hook (47) has an I-shaped structure; the left and right sides of the transfer hook (47) form a first accommodating space and a second accommodating space respectively. When the two reagent cards (51) are placed in the first accommodating space and the second accommodating space respectively, the transfer hook (47) can push the reagent card (51) between the discard position (42) and the incubation position (46) when it moves. The discarding position (42) is provided with two discarding through holes, which correspond to the first receiving space and the second receiving space in the vertical direction, respectively.

7. The multifunctional fully automatic colloidal gold detector according to claim 1, characterized in that, The X-axis sample feeding mechanism (200) includes a sample feeding support housing (74) and a sample feeding motion assembly; The sample feeding support housing (74) is provided with a second slide rail (76). The sample loading motion assembly includes an X-axis motion support (78), an X-axis motor (81), a fifth synchronous belt (82), an X-axis drive wheel (83), and an X-axis driven wheel (84). The second slide rail (76), the X-axis motor (81), the X-axis drive wheel (83), and the X-axis driven wheel (84) are all mounted on the sample feeding support housing (74); The two ends of the fifth synchronous belt (82) are respectively fitted onto the X-direction driving pulley (83) and the X-direction driven pulley (84); The X-axis motion bracket (78) is mounted on the second slide rail (76) and can slide on the second slide rail (76). The X-axis motion bracket (78) is connected to the fifth synchronous belt (82). The X-axis motor (81) and the X-axis drive wheel (83) are respectively installed on both sides of the sample feeding support housing (74), and the X-axis motor (81) can drive the X-axis drive wheel (83) to rotate and drive the fifth synchronous belt (82) to drive the X-axis motion bracket (78) to slide along the length of the second slide rail (76).

8. The multifunctional fully automatic colloidal gold detector according to claim 7, characterized in that, The sample dispensing motion assembly includes a plunger pump (75), a Z-axis motor (77), a third drive screw (79), a third slide rail (80), and a Z-axis motion support (85). The Z-axis motor (77) and the third slide rail (80) are both mounted on the X-axis motion bracket (78), and the Z-axis motion bracket (85) is mounted on the third slide rail (80) and can slide along the third slide rail (80); One end of the third drive screw (79) is connected to the Z-axis motor (77), and the other end of the third drive screw (79) is connected to the Z-axis motion bracket (85). When the Z-axis motor (77) rotates, the third drive screw (79) rotates, thereby driving the Z-axis motion bracket (85) to move vertically upward or downward along the third slide rail (80). The TIP needle bar (73) is mounted on the Z-axis motion bracket (85), and the plunger pump (75) is connected to the TIP needle bar (73) through a pipeline.

9. The multifunctional fully automatic colloidal gold detector according to claim 1, characterized in that, The TIP conveying mechanism (300) includes a TIP box (86), a TIP box conveying motor (87), a TIP box conveying drive wheel (88), a TIP box conveying driven wheel (89), a TIP box conveying support platform (90), a TIP box conveying linkage frame (91), and a TIP box synchronous belt (93). The TIP box conveying support platform (90) is provided with a TIP box support plate. The TIP box (86) is installed on the TIP box support plate via the TIP box slide plate (92). The TIP box conveying linkage frame (91) is installed below the TIP box support plate and extends outward and upward at both ends, respectively connecting to the two ends of the TIP box slide plate (92). The TIP box conveyor linkage frame (91) is connected to the TIP box synchronous belt (93); The TIP box conveyor motor (87), TIP box conveyor drive wheel (88), and TIP box conveyor driven wheel (89) are all mounted on the TIP box conveyor support platform (90), and the two ends of the TIP box synchronous belt (93) are respectively fitted onto the TIP box conveyor drive wheel (88) and the TIP box conveyor driven wheel (89); When the TIP box conveyor motor (87) rotates, it can drive the TIP box conveyor drive wheel (88) to rotate and drive the TIP box synchronous belt (93) to move, thereby driving the TIP box conveyor linkage frame (91) to drive the TIP box connecting slide plate (92) and TIP box (86) to move on the TIP box support plate.

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