A tilting and rotating device

By designing an inclined rotation device including a culture plate, an angle adjustment component, an inclination retaining component and a driving member, the problem of difficult to control the angle and force when manually shaking the culture medium is solved, and uniform shaking of the culture medium and stability of the culture medium are achieved.

CN112574858BActive Publication Date: 2025-05-02GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH CHINESE ACADEMY OF SCIENCES
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Patent Information

Application Number
CN202011551392.2
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2020-12-24
Publication Date
2025-05-02
Estimated Expiration
2040-12-24

AI Technical Summary

Technical Problem

In the prior art, when it is necessary to manually shake the culture medium in the Petri dish, the angle and force of the shaking are difficult to accurately control, resulting in the inability to shake the culture medium and the Petri dish is easily fallen off from the hand.

Method used

An inclined rotation device is designed, including a culture plate, an angle adjustment assembly, an inclination retaining assembly and a drive member. The angle adjustment component adjusts the inclination angle of the culture plate, the inclination angle holding component maintains the angle, and the culture plate is driven to rotate through the driving member to achieve uniform shake of the culture medium.

Benefits of technology

The culture medium is shaken evenly to ensure that the cells are evenly dispersed in the culture medium, and at the same time, the risk of the culture dish falling off during the shaking process is avoided.

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Abstract

The present invention relates to the technical field of cell biology experimental equipment, and in particular to a tilting and rotating device, which comprises a culture plate, an angle adjustment component, a tilt holding component and a driving member, wherein one end of the angle adjustment component is connected to the culture plate, and can adjust the angle between the culture plate and the horizontal plane; the tilt holding component is sleeved on the angle adjustment component, and one end abuts against the lower end surface of the culture plate, and is used to support the culture plate to maintain the angle with the horizontal plane; the other end of the angle adjustment component is transmission-connected to the output end of the driving member, and the driving member is used to drive the culture plate to rotate. By using the tilting and rotating device provided by the present invention, the culture plate can be adjusted to a certain tilt angle and the tilt angle of the culture plate can be maintained by the tilt holding component. After adding cell culture fluid, the culture dish on the culture plate is driven to rotate at a certain tilt angle and shake the cell culture fluid evenly through the uniform rotation of the driving member.
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Description

Technical Field

[0001] The present invention relates to the technical field of cell biology equipment, and in particular to a tilting and rotating device. Background Art

[0002] During the cell culture process, different culture fluids need to be added to the cell culture dish to promote cell development. In the prior art, after adding the culture fluid, the culture fluid in the culture dish needs to be manually shaken. However, since the shaking angle and strength are difficult to accurately control, the culture fluid is not shaken well, and the culture dish is easy to fall off from the hand during the shaking process. Summary of the invention

[0003] The purpose of the present invention is to provide a tilting and rotating device to solve the problem in the prior art that when the culture medium in the culture dish needs to be manually shaken, the shaking angle and strength are difficult to accurately control, resulting in the culture medium not being shaken properly and the culture dish easily falling off from the hands during the shaking process.

[0004] To achieve this object, the present invention adopts the following technical solutions:

[0005] A tilt-rotate device, comprising:

[0006] A culture plate, on which a culture dish is fixedly arranged;

[0007] An angle adjustment component, one end of which is connected to the culture plate and capable of adjusting the angle between the culture plate and a horizontal plane;

[0008] An inclination angle maintaining component is sleeved on the angle adjusting component, and one end of the inclination angle maintaining component is in contact with the lower end surface of the culture plate, so as to support the culture plate to maintain an angle with the horizontal plane;

[0009] A driving member, the other end of the angle adjustment component is transmission-connected to the output end of the driving member, and the driving member is used to drive the culture plate to rotate.

[0010] Preferably, the angle adjustment assembly includes a first connecting rod, a second connecting rod and a transmission adapter, one end of the first connecting rod is connected to the culture plate, and the other end is hinged to the second connecting rod, the upper end of the transmission adapter is hinged to the second connecting rod, and the transmission adapter is connected to the output end of the driving member.

[0011] Preferably, the transmission adapter comprises a first connecting plate and a second connecting plate arranged in parallel and spaced apart, the first connecting plate being provided with a first mounting groove, the second connecting plate being provided with a second mounting groove, the output end of the driving member being clamped between the first mounting groove and the second mounting groove, and the second connecting rod being hinged to both the first connecting plate and the second connecting plate.

[0012] Preferably, the inclination angle maintaining assembly includes an inclination support assembly, a telescopic maintaining assembly and a limit member, the telescopic maintaining assembly is sleeved on the angle adjustment assembly, the inclination support assembly is rotatably arranged on the telescopic maintaining assembly and sleeved on the angle adjustment assembly, the telescopic maintaining assembly can drive the inclination support assembly and thereby drive the culture plate to have a certain inclination angle with the horizontal plane, and the limit member is used to limit the telescopic height of the telescopic maintaining assembly.

[0013] Preferably, the tilt support assembly includes a support ring, the telescopic retaining assembly includes a first sleeve and a second sleeve, the first sleeve is slidably mounted on the second sleeve, the support ring is rotatably set on the first sleeve, the second sleeve is fixedly set on the driving member, the angle adjustment assembly is located in the second sleeve, and the limit member is used to limit the relative position between the first sleeve and the second sleeve.

[0014] Preferably, a protrusion is convexly provided on the outer peripheral surface of the first sleeve, one end of the limiting member abuts against the protrusion, and the other end of the limiting member is connected to the second sleeve.

[0015] Preferably, the second sleeve comprises a mounting portion and a sliding portion which are coaxially connected to each other, the first sleeve is movably sleeved on the sliding portion, the mounting portion is connected to the driving member, and the limiting member is fixedly disposed on the mounting portion.

[0016] Preferably, the culture plate is provided with a plurality of mounting grooves, and the culture dish is correspondingly arranged in each mounting groove.

[0017] Preferably, a cover plate is provided on the culture dish.

[0018] Preferably, the cover plate is hinged to the culture dish.

[0019] Beneficial effects of the present invention:

[0020] By using the tilting and rotating device provided by the present invention, the culture plate can be adjusted to a certain tilt angle and the tilt angle of the culture plate can be maintained by the tilt angle maintaining component. After adding the cell culture fluid, the culture dish on the culture plate is driven by the uniform rotation of the driving member to rotate at a certain tilt angle and uniformly to shake the cell culture fluid. Compared with the prior art which requires manual shaking of the culture fluid in the culture dish, the tilting and rotating device provided by the present invention can control the tilt angle and speed when shaking the cell culture fluid and the shaking is in place, so that the cells can be evenly dispersed on the cell culture fluid, and the culture dish is fixedly arranged on the culture plate and will not easily fall off the culture plate during the shaking process. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is an overall structural diagram of a tilt-rotation device of the present invention;

[0022] Figure 2 It is a sectional view of the overall structure of a tilt-rotation device of the present invention;

[0023] Figure 3 It is a connection structure diagram of a support ring and a first sleeve in a tilting and rotating device of the present invention.

[0024] In the figure:

[0025] 1. Culture plate;

[0026] 2. Angle adjustment assembly; 21. First connecting rod; 22. Second connecting rod; 23. Transmission adapter;

[0027] 3. Inclination holding assembly; 31. Support ring; 32. Telescopic holding assembly; 33. Positioning member; 321. First sleeve; 322. Second sleeve; 3211. Protrusion; 3221. Mounting portion; 3222. Sliding portion;

[0028] 4. Driving parts. DETAILED DESCRIPTION

[0029] The technical solution of the present invention is further described below in conjunction with the accompanying drawings and implementation methods. It is to be understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention. It should also be noted that, for ease of description, only the parts related to the present invention are shown in the accompanying drawings, rather than all of them.

[0030] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0032] like Figure 1-Figure 3 As shown, this embodiment provides a tilting and rotating device, including a culture plate 1, an angle adjustment component 2, a tilt holding component 3 and a driving member 4, wherein a culture dish is fixedly arranged on the culture plate 1, one end of the angle adjustment component 2 is connected to the culture plate 1, and can adjust the angle between the culture plate 1 and the horizontal plane; the tilt holding component 3 is sleeved on the angle adjustment component 2, and one end abuts against the lower end surface of the culture plate 1, and is used to support the culture plate 1 to maintain the angle with the horizontal plane; the other end of the angle adjustment component 2 is transmission-connected to the output end of the driving member 4, and the driving member 4 is used to drive the culture plate 1 to rotate. Using the tilting and rotating device provided in this embodiment, the culture plate 1 can be adjusted to a certain tilt angle and the tilt angle of the culture plate 1 can be maintained by the tilt holding component 3.

[0033] After adding the cell culture medium, the uniform rotation of the driving member 4 drives the culture dish on the culture plate 1 to rotate uniformly at a certain inclination angle, so that the added cell culture medium is evenly spread on the cells. Compared with the prior art which requires manual shaking of the culture medium in the culture dish, the tilting rotation device provided in this embodiment can control the inclination angle and speed when shaking the cell culture medium, and the shaking is in place, so that the cells can be evenly spread in the cell culture medium, and the culture dish will not fall off easily during the shaking process.

[0034] Specifically, the angle adjustment assembly 2 includes a first connecting rod 21, a second connecting rod 22 and a transmission adapter 23. One end of the first connecting rod 21 is connected to the culture plate 1, and the other end is hinged to the second connecting rod 22. The upper end of the transmission adapter 23 is hinged to the second connecting rod 22. The transmission adapter 23 is connected to the output end of the driving member 4. The inclination angle maintaining assembly 3 can drive the first connecting rod 21 to move up and down. At this time, the second connecting rod 22 rotates around the hinge with the transmission adapter 23, and the first connecting rod 21 rotates around the hinge with the second connecting rod 22 accordingly, so that the culture plate 1 can be rotated to tilt at a certain angle to the horizontal plane. Starting the driving member 4 can complete the tilt rotation and shake the cell culture medium.

[0035] Furthermore, the transmission adapter 23 includes a first connecting plate and a second connecting plate arranged in parallel and spaced apart. The first connecting plate is provided with a first mounting groove, and the second connecting plate is provided with a second mounting groove. The output end of the driving member 4 is clamped between the first mounting groove and the second mounting groove. The first connecting plate and the second connecting plate are tightened together by bolts to hold the output end of the driving member 4, so that the transmission connection is firm and the rotation is uniform and stable. At the same time, it is convenient for installation and disassembly. The second connecting rod 22 is hinged to the first connecting plate and the second connecting plate, and the angle adjustment is flexible.

[0036] Specifically, the inclination angle maintaining assembly 3 includes a tilting support assembly, a telescopic maintaining assembly 32 and a limiter 33. The telescopic maintaining assembly 32 is sleeved on the angle adjustment assembly 2. The tilting support assembly is rotatably arranged on the telescopic maintaining assembly 32 and sleeved on the angle adjustment assembly 2. The telescopic maintaining assembly 32 can drive the tilting support assembly and thus drive the culture plate 1 to have a certain inclination angle with the horizontal plane. The limiter 33 is used to limit the telescopic height of the telescopic maintaining assembly 32. Furthermore, the limiter 33 is a linear motor. The linear motor directly generates linear motion, has high positioning accuracy, is stable and reliable, and is easy to operate and maintain. In other embodiments, the limiter 33 can also use a fixing pin, and the fixing pin supports the telescopic maintaining assembly 32 to maintain a certain telescopic height.

[0037] Furthermore, the tilting support assembly includes a support ring 31, and the telescopic holding assembly 32 includes a first sleeve 321 and a second sleeve 322. The first sleeve 321 is slidably sleeved on the second sleeve 322, and the second sleeve 322 guides the telescopic sliding of the first sleeve 321 to ensure that the first sleeve 321 does not generate horizontal shaking during the telescopic process. The support ring 31 is rotatably set on the first sleeve 321 through two rotating pins, and can flexibly rotate with the tilt of the culture plate 1. The second sleeve 322 is fixedly set on the driving member 4, and the angle adjustment assembly 2 is located in the second sleeve 322 to prevent external dust from falling into the device and thus reducing the service life of the device. The output end of the linear motor is fixedly set on the second sleeve 322, and the other end abuts on the first sleeve 321. The linear motor can drive the first sleeve 321 to reciprocate in the vertical direction to adjust the tilt angle of the culture plate 1.

[0038] Furthermore, a protrusion 3211 is convexly provided on the outer peripheral surface of the first sleeve 321 , and the linear motor abuts against the protrusion 3211 , so that the transmission is stable when the first sleeve 321 is extended or retracted.

[0039] Furthermore, the second sleeve 322 includes a mounting portion 3221 and a sliding portion 3222 which are coaxially connected to each other. The first sleeve 321 is movably sleeved on the sliding portion 3222 and can abut against the mounting portion 3221. The mounting portion 3221 can limit the first sleeve 321. At the same time, the mounting portion 3221 is fixedly connected to the driving member 4 by bolts to increase the overall stability of the device.

[0040] Specifically, a plurality of mounting grooves are provided on the culture plate 1, and a culture dish is correspondingly arranged in each mounting groove, so that the shaking operation of multiple groups of cell culture fluids can be completed at one time.

[0041] Furthermore, a cover is provided on the culture dish, which is hinged on the culture dish and can open and close the culture dish to prevent the cell culture fluid and cells from being thrown out of the culture dish during the shaking operation.

[0042] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the embodiments here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A tilting and rotating device, characterized in that: include: A culture plate (1), wherein a culture dish is fixedly arranged on the culture plate (1); An angle adjustment component (2), one end of the angle adjustment component (2) being connected to the culture plate (1) and capable of adjusting the angle between the culture plate (1) and a horizontal plane; An inclination angle maintaining component (3) is sleeved on the angle adjustment component (2), and one end of the component abuts against the lower end surface of the culture plate (1), so as to support the culture plate (1) to maintain an angle with a horizontal plane; A driving member (4), the other end of the angle adjustment assembly (2) being drivingly connected to the output end of the driving member (4), and the driving member (4) being used to drive the culture plate (1) to rotate; The angle adjustment assembly (2) comprises a first connecting rod (21), a second connecting rod (22) and a transmission adapter (23); one end of the first connecting rod (21) is connected to the culture plate (1), and the other end is hinged to the second connecting rod (22); the upper end of the transmission adapter (23) is hinged to the second connecting rod (22); the transmission adapter (23) is connected to the output end of the driving member (4); and the inclination angle maintaining assembly (3) is capable of driving the first connecting rod (21) to move up and down; The tilt angle holding assembly (3) comprises a tilt support assembly, a telescopic holding assembly (32) and a limiter (33); the telescopic holding assembly (32) is sleeved on the angle adjustment assembly (2); the tilt support assembly is rotatably arranged on the telescopic holding assembly (32) and sleeved on the angle adjustment assembly (2); the telescopic holding assembly (32) can drive the tilt support assembly and thus drive the culture plate (1) to form a certain tilt angle with the horizontal plane; the limiter (33) is used to limit the telescopic height of the telescopic holding assembly (32); The tilt support assembly comprises a support ring (31); the telescopic holding assembly (32) comprises a first sleeve (321) and a second sleeve (322); the first sleeve (321) is slidably sleeved on the second sleeve (322); the support ring (31) is rotatably arranged on the first sleeve (321); the second sleeve (322) is fixedly arranged on the driving member (4); the angle adjustment assembly (2) is located in the second sleeve (322); and the limiting member (33) is used to limit the relative position between the first sleeve (321) and the second sleeve (322).

2. The tilt-rotation device according to claim 1, characterized in that: The transmission adapter (23) comprises a first connecting plate and a second connecting plate which are arranged in parallel and spaced apart from each other, the first connecting plate being provided with a first mounting groove, the second connecting plate being provided with a second mounting groove, the output end of the driving member (4) being clamped between the first mounting groove and the second mounting groove, and the second connecting rod (22) being hingedly connected to both the first connecting plate and the second connecting plate.

3. The tilt-rotate device according to claim 1, characterized in that: A protrusion (3211) is convexly provided on the outer peripheral surface of the first sleeve (321), one end of the limiting member (33) abuts against the protrusion (3211), and the other end of the limiting member (33) is connected to the second sleeve (322).

4. The tilt-rotation device according to claim 3, characterized in that: The second sleeve (322) comprises a mounting portion (3221) and a sliding portion (3222) which are coaxially connected to each other, the first sleeve (321) is movably sleeved on the sliding portion (3222), the mounting portion (3221) is connected to the driving member (4), and the limiting member (33) is fixedly arranged on the mounting portion (3221).

5. The tilt-rotate device according to claim 1, characterized in that: The culture plate (1) is provided with a plurality of mounting grooves, and the culture dish is correspondingly arranged in each mounting groove.

6. The tilt-rotate device according to claim 5, characterized in that: The culture dish is provided with a cover plate.

7. The tilt-rotate device according to claim 6, characterized in that: The cover plate is hinged to the culture dish.

Citation Information

Patent Citations

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