Method for determining content of six anticoccidial drugs in feed by liquid chromatography

A liquid chromatography, anti-coccidial technology, applied in the direction of measurement device, material separation, analysis of materials, etc., can solve problems such as unseen detection methods, and achieve good repeatability.

CN113484433AActive Publication Date: 2021-10-08GUIZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2021-10-08

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Abstract

The invention relates to a method for detecting the content of six anticoccidial drugs, namely clopidol, dinitolmide, nicarbazin, sulfaquinoxaline, chlorcucurbita hydrochloride and diclazuril in chicken feed, premix and feed additives. The method mainly comprises the following steps that: extraction isperformed with using 0.1% trichloroacetic acid acetonitrile; purification is performed with 50mg of PSA; redissolving is performed with methanol and a 5mmol / L ammonium acetate aqueous solution in a volume ratio of 8: 2 as a redissolving solution; and an Agilent Extend-C18 (250 mm * 4.6 mm i. D., 5 [mu] m) chromatographic column is used as an analytical column, 270 nm and 340 nm wavelengths are selected, the sample size is 20 [mu] L, the column temperature is 25 DEG C, and an Agilent 1260 high performance liquid chromatography system (Agilent, DAD) is used for analysis. The invention aims to establish a novel, simple and convenient analysis method for rapidly determining various anticoccidial drugs in chicken feed, premix and feed additive by a high performance liquid chromatography detection method with good repeatability. The method is simple and convenient to operate and good in repeatability, and saves time and cost.
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Description

technical field

[0001] The invention belongs to the construction of a detection method for anticoccidial drugs in feed, and in particular relates to the use of clonazine, dinitramide, sulfaquinoxaline, prophenylguanidine, nicarbazine and diclazuril in chicken feed, premixed Content detection in feed and feed additives. Background technique

[0002] Coccidiosis mainly parasitizes the intestinal tract of the host (chicken, duck, pigeon, cattle, sheep, etc.), and causes damage to the liver (rabbit) and kidney (goose) to varying degrees. It is one of the major diseases in the poultry farming industry , causing billions of dollars in losses every year. Anticoccidiostats are mainly used for the prevention of coccidiosis, which can improve feed utilization, reduce the risk of secondary diseases, and promote growth. It is inevitable that unscrupulous merchants will use this feature to seek more benefits, resulting in anticoccidial drugs abuse. However, anticoccidial drugs are hig...

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Embodiment Construction

[0024] The present invention will be described below by means of embodiments and accompanying drawings.

[0025] A method for determining the content of six coccidial drugs in feed by liquid chromatography, comprising the following steps: accurately weigh 1g (±0.01g) of uniformly ground feed in a 15mL polypropylene centrifuge tube, add an appropriate amount of mixed standard working solution , vortexed to mix and then stand at room temperature for 30min, to be extracted. Add 5mL 0.1% trichloroacetic acid acetonitrile solution to the centrifuge tube, vortex fully, sonicate for 10min, shake for 30min, centrifuge at 10000rpm for 10min, transfer the supernatant to another centrifuge tube, and wash the residue with 5mL0.1% trichloroacetic acid acetonitrile The solution was repeatedly extracted once, mixed and centrifuged, and the supernatant was combined to be purified. Transfer 1 mL of the above solution to a 2 mL plastic centrifuge tube pre-filled with 50 mg of PSA filler, vorte...