A MOFs nanoscale enzyme and application thereof in detection of new coronavirus

By modifying the CD147 protein on the surface of MOF nanozymes and utilizing its specific binding to the novel coronavirus, a label-free colorimetric detection method is achieved by catalyzing chromogenic molecules. This solves the sensitivity and equipment limitations of novel coronavirus detection and enables rapid, visualized, and highly sensitive detection.

CN115353557BActive Publication Date: 2026-05-08XI AN JIAOTONG UNIV
2 Cites 0 Cited by

Patent Information

Application Number
CN202211008677.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-08-22
Publication Date
2026-05-08
Estimated Expiration
2042-08-22

AI Technical Summary

Technical Problem

Existing COVID-19 testing methods lack sensitivity and specificity, and the equipment is expensive and inflexible, making it difficult to achieve large-scale, multi-sample testing. Enzymes are easily inactivated under harsh conditions, limiting the application of colorimetric methods.

Method used

MOF nanozymes are used to modify the surface of CD147 protein by forming a MOF material with metal ions and terephthalic acid. The MOF material is then modified with its peroxidase-like properties to catalyze the colorimetric molecules TMB, ABTS, OPD and PPD. Combined with the specific binding of CD147 to the SARS-CoV-2 virus, label-free, rapid and highly sensitive colorimetric detection is achieved.

Benefits of technology

It enables rapid, visualized, and highly sensitive detection of SARS-CoV-2, with a detection limit as low as 3 PFU/mL, simplifying the detection process, reducing costs, and making it suitable for operation in remote areas.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

A kind of MOFs nano-enzyme and application in new coronavirus detection, it relates to biological detection method field, MOFs-based nano-enzyme has peroxidase-like effect, can catalyze color developing agent discoloration;After the specific protein CD147 is modified on the surface of nano-enzyme, using the specific binding of CD147 and the S protein overexpressed on the surface of new coronavirus, new coronavirus can be efficiently combined on the surface of nano-enzyme to inhibit the catalytic color development of nano-enzyme;Based on the principle, the quantitative and qualitative detection of new coronavirus can be realized by colorimetric method;The present application realizes the rapid, high-sensitivity and visual detection of new coronavirus by the inherent catalytic advantage of nano-enzyme and the specific recognition and binding capacity of CD147 to new coronavirus without additional labeling process, and can effectively identify new coronavirus infection.
Need to check novelty before this filing date? Find Prior Art