A color calibration card based on fluorescence and its application

Abstract

The present invention belongs to the technical field of fluorescent materials and relates to a fluorescence-based color calibration card. The fluorescence-based color calibration card includes a shell portion and a fluorescent material module portion; the fluorescent material module portion is liquid or colloid; the fluorescent material module portion includes a fluorescent dye and a solvent; or, the fluorescent material module portion includes a fluorescent dye, a solvent, and a thermogel substance; the shell portion includes: a substrate and a cover plate, a filling groove is provided on the surface of the substrate, and the cover plate covers the opening of the filling groove to confine the fluorescent material module portion within the filling groove. The fluorescence-based color calibration card provided by the present invention can be used for parameter correction of fluorescent endoscope equipment, judgment of the operating status of fluorescent endoscope equipment, and auxiliary adjustment of the operating status of fluorescent endoscope equipment under different fluorescence modes, making up for the defect that the current fluorescent endoscope lacks a matching color calibration card.

Description

Technical Field

[0001] The invention belongs to the technical field of fluorescent materials and relates to a fluorescence-based color calibration card and application thereof. Background Art

[0002] Fluorescence endoscope is a new type of diagnostic equipment that uses laser intrinsic fluorescence spectroscopy technology to enter the cavity for diagnosis through various endoscopes. It automatically identifies and diagnoses based on the intrinsic fluorescence spectral characteristics of human tissue, and can immediately indicate whether the tested tissue is normal tissue. It can also distinguish benign and malignant lesions of the tested tissue, thereby improving the diagnosis rate of early cancer and dysplasia.

[0003] Currently, pre-use calibration of fluorescence endoscope equipment parameters relies on operator experience. Common methods for parameter setting and instrument status determination primarily involve machine self-tests, in vitro fluorescent dye smear testing, and adjustments during use. These methods not only suffer from inaccurate parameter settings, waste of fluorescent dye, and difficulty in standardized operation, but also pose risks for clinical examinations and intraoperative navigation when the instrument is not used under appropriate conditions, and there is also the possibility of damage to the device probe. Therefore, there is an urgent need to develop a fluorescence calibration card that can be used with a variety of fluorescence equipment, including fluorescence endoscopes, and scientific research equipment.

[0004] Color calibration charts have become essential accessories for a variety of other imaging and photographic equipment, including white balance calibration charts and standard color palettes for cameras and mobile phones, color calibration charts for printing and scanning equipment, and sensitive gradient calibration charts for military near-infrared or thermal imaging lenses. However, there is a lack of supporting color calibration equipment for fluorescent endoscopes, which are now widely used in hospitals and research institutes. Summary of the Invention

[0005] In response to the deficiencies in the prior art, the present invention provides a fluorescence-based color calibration card, which can be used for parameter correction, operating status judgment and auxiliary adjustment of the operating status of fluorescence endoscope equipment under different fluorescence modes.

[0006] One aspect of the present invention provides a fluorescence-based color calibration card, the fluorescence-based color calibration card comprising a shell portion and a fluorescent material module portion; the fluorescent material module portion is liquid or colloid;

[0007] When the fluorescent material module part is liquid, it includes fluorescent dye and solvent; when the fluorescent material module part is colloid, it includes fluorescent dye, solvent and thermal gel material.

[0008] Preferably, the fluorescent dye is one or more of ultraviolet fluorescent dye, visible light fluorescent dye, and near-infrared fluorescent dye.

[0009] Preferably, the ultraviolet fluorescent dye is a small molecule fluorescent dye with an emission wavelength of 280 nm ≤ < 400 nm and / or an inorganic fluorescent dye with an emission wavelength of 280 nm ≤ < 400 nm, including but not limited to 1,8-naphthalene diimide.

[0010] Preferably, the visible light fluorescent dye is a small molecule fluorescent dye with an emission wavelength of 400 nm ≤ < 780 nm and / or an inorganic fluorescent dye with an emission wavelength of 400 nm ≤ < 780 nm, including but not limited to coumarin fluorescent dyes, fluorescein dyes, rhodamine dyes, pyrene fluorescent dyes, cyanine fluorescent dyes, BODIPY fluorescent dyes, carbon quantum dots, and zinc-dimethylpyridinamine (ZDPA) fluorescent dyes.

[0011] Preferably, the near-infrared fluorescent dye is a small molecule fluorescent dye with an emission wavelength of 780 nm ≤ < 1700 nm and / or an inorganic fluorescent dye with an emission wavelength of 780 nm ≤ < 1700 nm, including but not limited to cyanine fluorescent dyes, BODIPY fluorescent dyes, AIE fluorescent dyes, and DAD fluorescent dyes.

[0012] Preferably, the solvent is a liquid capable of dissolving fluorescent dyes, including one or more of water, inorganic salt solution and organic solvent.

[0013] Preferably, the inorganic salt solution includes, but is not limited to, one or more of a sodium salt solution, a potassium salt solution, an iodine salt solution, a bromide salt solution, and a chloride salt solution.

[0014] Preferably, the organic solvent includes, but is not limited to, one or more of ethanol, methanol, ethanol, dichloromethane, chloroform, ethyl acetate, and petroleum ether.

[0015] Preferably, the thermogel material is a material that can form a gel after being heated when prepared as a suspension or solution, including but not limited to one or more of agar, chitosan, carrageenan, curdlan, xanthan gum, gellan gum, and sodium carboxymethyl cellulose.

[0016] Preferably, the fluorescent material module further includes an anti-fluorescence quenching agent.

[0017] The anti-fluorescence quenching agent is a substance that slows down the fluorescence quenching of fluorescent dyes, including but not limited to one or more of anti-fluorescence quenching inorganic salts, anti-fluorescence quenching organic small molecules, and anti-fluorescence quenching polymer compounds.

[0018] Preferably, the inorganic salts that resist fluorescence quenching include, but are not limited to, one or more of sodium metabisulfite, sodium dithionite, and sodium bicarbonate.

[0019] Preferably, the organic small molecules that resist fluorescence quenching include, but are not limited to, one or both of ethyl peroxide and dimethyl sulfide.

[0020] Preferably, the high molecular weight compound resistant to fluorescence quenching includes, but is not limited to, one or more of polyethylene glycol, distearoylphosphatidylethanolamine, polyacrylamide, and polypeptide.

[0021] Preferably, when the fluorescent material module portion is liquid, the preparation method of the fluorescent material module portion includes the following steps: adding the fluorescent dye to the solvent, shaking it thoroughly to make it uniform, then extracting and pouring it into the filling groove of the shell portion until it is completely filled, and then sealing it;

[0022] When the fluorescent material module portion is a colloid, the preparation method of the fluorescent material module portion includes the following steps: adding a fluorescent dye to a solvent, heating and fully shaking it, heating an aqueous solution of a thermogel material, mixing the two solutions, extracting and pouring them into the filling groove of the shell portion until it is completely filled, cooling it into a colloid, and then sealing it.

[0023] Preferably, the fluorescent dye is added to the solvent to form a 0.05-1 mg / ml fluorescent dye solution; the aqueous solution of the thermogel material is formed by dissolving or dispersing the thermogel material in the aqueous solution, and the concentration of the aqueous solution of the thermogel material is 0.5-5 wt%; the fluorescent dye solution and the aqueous solution of the thermogel material are mixed in a volume ratio of 5:1-1:5.

[0024] Preferably, the heating temperature is 35-90°C.

[0025] Preferably, the housing portion comprises:

[0026] A substrate, wherein a filling groove is formed on a surface of the substrate, and the filling groove extends from the surface of the substrate in a thickness direction of the substrate;

[0027] A cover plate covers the opening of the filling groove so as to partially confine the fluorescent material module within the filling groove, and a portion of the substrate corresponding to the filling groove is configured as a transparent portion.

[0028] Preferably, the substrate and cover plate are made of one or more materials selected from the group consisting of glass and organic glass.

[0029] Preferably, the glass includes but is not limited to one or more of borosilicate glass, quartz glass, and tempered glass; the organic glass includes but is not limited to one or more of polydimethylsiloxane (PDMS), polymethyl methacrylate (PMMA), and polycarbonate (PC).

[0030] Preferably, the edge portion of the filling slot is configured as a stepped structure, and the cover plate is adhesively connected to the stepped surface of the filling slot.

[0031] Preferably, the stepped surface of the filling groove is provided with at least one annular groove and the cover plate is provided with at least one annular protrusion complementary to the annular groove, or the stepped surface of the filling groove is provided with at least one annular protrusion and the cover plate is provided with at least one annular groove complementary to the annular protrusion; wherein the annular protrusion is embedded in the annular groove so that the cover plate is sealedly connected to the stepped surface of the filling groove.

[0032] Preferably, the substrate is provided with a threaded hole, one end of the threaded hole is connected to the outside world and the other end is connected to the filling groove, and a detachable screw is sealed in the threaded hole; or, the substrate is provided with an injection hole and a cover plate, one end of the injection hole is connected to the outside world and the other end is connected to the filling groove, and the cover plate covers the injection hole.

[0033] An application of a fluorescence-based color calibration card, wherein the fluorescence-based color calibration card is used for parameter correction of a fluorescence endoscope device, operating status judgment of a fluorescence endoscope device, and auxiliary adjustment of the operating status of a fluorescence endoscope device under different fluorescence modes.

[0034] For example, the fluorescence-based color calibration card can be used to judge the working status of the light source of the fluorescence endoscope device, wherein the light source includes a laser light source and a white light source. The fluorescence-based color calibration card can be used to judge the operating status of the lens in the fluorescence mode of the fluorescence endoscope device. When the fluorescence endoscope device has a fluorescent pseudo-color mode, the fluorescence-based color calibration card can be used to judge the imaging status in the pseudo-color mode of the fluorescence endoscope device. The fluorescence-based color calibration card can be used as a reference for selecting the laser power gear of the fluorescence endoscope device. The fluorescence-based color calibration card can be used as a reference for selecting the lens aperture size of the fluorescence endoscope device. The fluorescence-based color calibration card can be used as a reference for selecting the current status and operating mode of the fluorescence endoscope device.

[0035] Compared with the prior art, the present invention has the following beneficial effects:

[0036] (1) The present invention provides a fluorescence-based color calibration card, which is the first of its kind in China and can be used for parameter calibration of fluorescence endoscope equipment, determination of the operating status of fluorescence endoscope equipment, and auxiliary adjustment of the operating status of fluorescence endoscope equipment under different fluorescence modes, thus making up for the defect that current fluorescence endoscopes lack matching color calibration cards.

[0037] (2) The fluorescent material module portion of the fluorescence-based color calibration card provided by the present invention is liquid or colloid, which is beneficial for slowing down the quenching of the fluorescent material;

[0038] (3) The fluorescent color calibration card of the present invention can effectively seal the fluorescent material module portion after the edge of the cover plate and the step surface of the filling groove are engaged through the annular protrusion and the annular groove, thereby avoiding the defects of partial leakage of the fluorescent material module and redissolution of the glue;

[0039] (4) A threaded hole or an injection hole is provided on the substrate of the shell portion, and the solution is injected into the filling tank through the threaded hole or the injection hole to form the fluorescent material module portion;

[0040] (5) The fluorescence-based color calibration card of the present invention is applicable to ultraviolet fluorescent dyes, visible light fluorescent dyes, and near-infrared fluorescent dyes, which greatly expands the scope of application. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 Schematic diagram of the fluorescence-based color calibration card of the present invention;

[0042] Figure 2 This is an exploded view of the structure of the fluorescence-based color calibration card of the present invention;

[0043] Figure 3 This is a schematic diagram of the back side of the substrate of the fluorescence-based color calibration card of the present invention;

[0044] Figure 4 Schematic diagram of the structure of the threaded hole and filling slot of the fluorescence-based color calibration card of the present invention;

[0045] Figure 5 This is a schematic structural diagram of the engagement between the cover plate and the stepped surface of the fluorescence-based color calibration card of the present invention.

[0046] In the figure, 100, substrate; 110, filling groove; 120, stepped surface; 130, threaded hole; 140, screw; 150, injection hole; 160, cover sheet; 200, cover plate; 210, transparent part; 300, annular groove; 400, annular protrusion. DETAILED DESCRIPTION

[0047] The technical scheme of the present invention is further described below by specific examples and accompanying drawings. It should be understood that the specific embodiments described herein are only used to help understand the present invention and are not intended to be limiting of the present invention. Unless otherwise specified, the raw materials used in the embodiments of the present invention are all commonly used raw materials in the art, and the methods used in the embodiments are all conventional methods in the art.

[0048] like Figure 1-5As shown, the fluorescence-based color calibration card provided by the present invention includes a shell portion and a fluorescent material module portion. The shell portion includes: a substrate 100 and a cover plate 200. The surface of the substrate 100 is provided with a filling groove 110. The filling groove 110 extends from the surface of the substrate 100 in the thickness direction of the substrate 100. That is, the filling groove 110 is a groove structure formed by an inward depression on the surface of the substrate 100. The cover plate 200 covers the opening of the filling groove 110, thereby confining the fluorescent material module portion within the filling groove 110. The portion of the substrate 100 corresponding to the filling groove 110 is configured as a transparent portion 210. At least the portion of the entire cover plate 200 corresponding to the filling groove 110 is configured as the transparent portion 210. In an actual structure, the entire cover plate 200 can be a transparent structure.

[0049] The fluorescent material module is a liquid or colloid. When the fluorescent material module is a liquid, it includes a fluorescent dye and a solvent. When the fluorescent material module is a colloid, it includes a fluorescent dye, a solvent, and a thermogel. The fluorescent material module is filled in the filling groove 110 of the housing.

[0050] The fluorescence-based color calibration card provided by the present invention has a filling groove 110 formed on the surface of the substrate 100 and the opening of the filling groove 110 is covered by a cover plate 200, so that the filling groove 110 actually forms a closed cavity. The filling groove 110 can accommodate a fluorescent material module portion in a liquid state or a colloidal state. The portion of the cover plate 200 corresponding to the filling groove 110 is set as a transparent portion 210, and the transparent portion 210 serves as a window for the instrument to perform parameter correction and auxiliary adjustment through the fluorescent material module portion.

[0051] The fluorescence-based color calibration card provided by the present invention is preferably, Figure 1 、 Figure 2 、 Figure 4 、 Figure 5 As shown, the edge portion of the filling slot 110 is configured as a stepped structure, and the edge of the cover plate 200 is connected to the stepped surface 120 of the filling slot 110. Preferably, the edge of the cover plate 200 is connected to the stepped surface 120 of the filling slot 110 by adhesive bonding.

[0052] In order to improve the sealing performance of the filling groove 110, the stepped surface 120 of the filling groove 110 is provided with at least one annular groove 300 and the cover plate 200 is provided with at least one annular protrusion 400 complementary to the annular groove 300, or the stepped surface 120 of the filling groove 110 is provided with at least one annular protrusion 400 and the cover plate 200 is provided with at least one annular groove 300 complementary to the annular protrusion 400; wherein, the annular protrusion 400 is embedded in the annular groove 300 so that the cover plate 200 is sealedly connected to the stepped surface 120 of the filling groove 110.

[0053] The purpose of connecting the cover plate 200 and the filling groove 110 by engaging the annular protrusion 400 with the annular groove 300 is to improve the sealing of the filling groove 110 and prevent the fluorescent material module part from reacting with the glue. Specifically, due to the engagement and sealing of the annular protrusion 400 and the annular groove 300, the liquid or colloidal fluorescent material module part in the filling groove 110 is effectively prevented from leaking out; since the cover plate 200 and the stepped surface 120 are connected together by glue, the fluorescent material module part is easily in contact with the glue, causing the glue to re-dissolve, which not only destroys the connection relationship between the cover plate 200 and the substrate 100, but also causes the fluorescent material module part to be contaminated. After the edge of the cover plate 200 and the stepped surface of the filling groove 110 are engaged with the annular protrusion 400 and the annular groove 300, it is possible to effectively seal and isolate the fluorescent material module part and the glue, thereby avoiding the defects of leakage of the fluorescent material module part and re-dissolution of the glue.

[0054] like Figure 1-4 As shown, the substrate 100 is provided with a threaded hole 130 , one end of the threaded hole 130 is communicated with the outside and the other end is communicated with the filling slot 110 , and a detachable screw 140 is sealed in the threaded hole 130 .

[0055] like Figure 1-3 As shown, the substrate 100 is provided with an injection hole 150 and a cover sheet 160 . One end of the injection hole 150 is connected to the outside and the other end is connected to the filling tank 110 . The cover sheet 160 covers the injection hole 150 .

[0056] When the fluorescent material module part is liquid, the preparation method of the fluorescent material module part includes the following steps: adding the fluorescent dye to the solvent, shaking it evenly, and then extracting the solution, injecting it into the filling tank 110 through the threaded hole 130 or the injection hole 150 until the filling tank 110 is completely filled, and then screwing the screw 140 into the threaded hole 130 to seal it, or using a cover sheet 160 to seal the injection hole 150 with glue.

[0057] When the fluorescent material module portion is a colloid, the preparation method of the fluorescent material module portion includes the following steps: adding a fluorescent dye to a solvent, heating and fully shaking it, heating an aqueous solution of a thermogel material, mixing the two solutions, extracting the mixed liquid, and injecting it into the filling tank 110 through the threaded hole 130 or the injection hole 150 until the filling tank 110 is completely filled. After cooling into a colloid, screwing a screw 140 into the threaded hole 130 to seal it, or using a cover sheet 160 to seal the injection hole 150 with glue.

[0058] Example 1

[0059] First, a shell part is prepared, wherein a base plate of the shell part is provided with a threaded hole and a screw capable of sealing the screw hole;

[0060] Add ICG fluorescent dye to water and shake thoroughly until it is completely dissolved to form an ICG aqueous solution with a concentration of 0.15 mg / ml. Use an injection to draw 20 ml of the ICG aqueous solution and inject it into the filling groove of the shell part through the threaded hole until the filling groove is completely filled. Then screw the screw into the threaded hole to seal it to obtain a fluorescence-based color calibration card.

[0061] Example 2

[0062] First, a shell part is prepared, wherein the base plate of the shell part is provided with a material injection hole and a cover sheet;

[0063] ICG fluorescent dye was added to water and shaken thoroughly until completely dissolved to form an ICG aqueous solution with a concentration of 0.1 mg / ml. 20 ml of the ICG aqueous solution was drawn with an injection syringe and injected into the filling slot of the shell part through the injection hole until the filling slot was completely filled. Then, a cover sheet was used to seal the injection hole with glue to obtain a fluorescence-based color calibration card.

[0064] Example 3

[0065] First, a shell part is prepared, wherein a base plate of the shell part is provided with a threaded hole and a screw capable of sealing the screw hole;

[0066] ICG fluorescent dye was added to water, heated to 60°C and shaken thoroughly until completely dissolved to form an ICG aqueous solution with a concentration of 0.2 mg / ml, and the temperature was maintained; a 2 wt% agar aqueous suspension was heated to 90°C and then cooled to 60°C; 10 ml of the ICG aqueous solution and 10 ml of the agar aqueous suspension were mixed, extracted with an injection, and injected into the filling groove of the shell part through the threaded hole until the filling groove was completely filled. After cooling into a gel, a screw was screwed into the threaded hole to seal it to obtain a fluorescence-based color calibration card.

[0067] Example 4

[0068] First, a shell part is prepared, wherein the base plate of the shell part is provided with a material injection hole and a cover sheet;

[0069] ICG fluorescent dye is added to water, heated to 60°C and shaken thoroughly until completely dissolved to form an ICG aqueous solution with a concentration of 0.3 mg / ml, and the temperature is maintained; a 3wt% agar aqueous suspension is heated to 90°C and then cooled to 60°C; 10 ml of the ICG aqueous solution and 10 ml of the agar aqueous suspension are mixed, extracted with an injection, and injected into the filling groove of the shell part through the injection hole until the filling groove is completely filled. After cooling into a gel, the injection hole is sealed with glue using a cover sheet to obtain a fluorescence-based color calibration card.

[0070] Example 5

[0071] First, a shell part is prepared, wherein the base plate of the shell part is provided with a material injection hole and a cover sheet;

[0072] ICG fluorescent dye was added to water, and anti-fluorescence quencher peroxyethyl ether (added in an amount of 10 -3 eq.), heated to 60°C and shaken thoroughly until completely dissolved to form an ICG aqueous solution with a concentration of 0.4 mg / ml, and the temperature was maintained; a 2 wt% curdlan glue suspension was heated to 85°C and then cooled to 60°C; 10 ml of the ICG aqueous solution and 10 ml of the curdlan glue suspension were mixed, extracted with an injection syringe, and injected into the filling slot of the shell portion through the injection hole until the filling slot was completely filled. After cooling to form a gel, the injection hole was sealed with glue using a cover sheet to obtain a fluorescence-based color calibration card.

[0073] Example 6-Example 19

[0074] The raw material ratios and compositions of Examples 6-19 are shown in Table 1, and the rest are the same as in Example 5.

[0075] Table 1 Composition of the raw materials in Examples 6 to 19

[0076]

[0077] *The mixing ratio refers to the volume ratio of the fluorescent dye solution to the aqueous solution of the thermogel material.

[0078] The fluorescence-based color calibration cards provided in Examples 1 to 19 can be successfully used for parameter correction of fluorescence endoscope equipment, operating status judgment of fluorescence endoscope equipment, and auxiliary adjustment of the operating status of fluorescence endoscope equipment under different fluorescence modes.

[0079] The fluorescence-based color calibration card of the present invention is provided with an annular groove on the stepped surface of the filling groove of the shell part, and an annular protrusion complementary to the annular groove is provided on the cover plate. The complementary connection between the annular groove and the annular protrusion ensures the sealing of the fluorescent material module part, which is beneficial to improving the service life of the color calibration card.

[0080] Finally, it should be noted that the specific embodiments described herein are merely illustrative of the spirit of the present invention and are not intended to limit the manner in which the present invention is intended to be implemented. Persons skilled in the art may make various modifications, additions, or substitutions to the described embodiments, and it is not necessary or possible to provide a comprehensive list of all possible embodiments. However, any obvious changes or modifications arising from the essential spirit of the present invention remain within the scope of protection of the present invention, and interpreting them as any additional limitations would be contrary to the spirit of the present invention.

Claims

1. A fluorescence-based color calibration card, characterized in that: The fluorescence-based color calibration card is used for parameter calibration of a fluorescence endoscope device, determination of the operating status of a fluorescence endoscope device, or auxiliary adjustment of the operating status of a fluorescence endoscope device under different fluorescence modes; The fluorescence-based color calibration card includes a shell portion and a fluorescent material module portion; The housing portion includes: a substrate and a cover; a filling groove is opened on the surface of the substrate, and the filling groove extends from the surface of the substrate along the thickness direction of the substrate; The cover plate covers the opening of the filling groove so as to confine the fluorescent material module portion within the filling groove, and a portion of the substrate corresponding to the filling groove is configured as a transparent portion; The edge portion of the filling slot is configured as a stepped structure, and the cover plate is adhesively connected to the stepped surface of the filling slot; The step surface of the filling groove is provided with at least one annular groove, and the cover plate is provided with at least one annular protrusion complementary to the annular groove, or the step surface of the filling groove is provided with at least one annular protrusion, and the cover plate is provided with at least one annular groove complementary to the annular protrusion; wherein the annular protrusion is embedded in the annular groove so that the cover plate is sealed to the step surface of the filling groove; The fluorescent material module part is liquid or colloid; When the fluorescent material module part is liquid, it includes fluorescent dye and solvent; when the fluorescent material module part is colloid, it includes fluorescent dye, solvent and thermal gel material.

2. The fluorescence-based color calibration card according to claim 1, characterized in that: The fluorescent dye is one or more of ultraviolet fluorescent dye, visible light fluorescent dye, and near-infrared fluorescent dye.

3. The fluorescence-based color calibration card according to claim 1, characterized in that: The solvent is one or more of water, inorganic salt solution and organic solvent.

4. The fluorescence-based color calibration card according to claim 1, characterized in that: The thermogel material is one or more of agar, chitosan, carrageenan, curdlan, xanthan gum, gellan gum, and sodium carboxymethyl cellulose.

5. The fluorescence-based color calibration card according to claim 1, characterized in that: The fluorescent material module also includes an anti-fluorescence quenching agent.

6. The fluorescence-based color calibration card according to claim 1, characterized in that: When the fluorescent material module portion is liquid, the preparation method of the fluorescent material module portion includes the following steps: adding fluorescent dye to a solvent, shaking it thoroughly to make it uniform, then extracting and pouring it into the filling groove of the shell portion until it is completely filled, and then sealing it; When the fluorescent material module portion is a colloid, the preparation method of the fluorescent material module portion includes the following steps: adding a fluorescent dye to a solvent, heating and fully shaking it, heating an aqueous solution of a thermogel material, mixing the two solutions, extracting and pouring them into the filling groove of the shell portion until it is completely filled, cooling it into a colloid, and then sealing it.

7. The fluorescence-based color calibration card according to claim 6, characterized in that: The heating temperature is 35-90℃.

8. The fluorescence-based color calibration card according to claim 1, characterized in that: The substrate is provided with a threaded hole, one end of which is connected to the outside world and the other end is connected to the filling groove, and a detachable screw is sealed in the threaded hole; or the substrate is provided with an injection hole and a cover plate, one end of which is connected to the outside world and the other end is connected to the filling groove, and the cover plate covers the injection hole.

9. An application of the fluorescence-based color calibration card according to claim 1, characterized in that: The fluorescence-based color calibration card is used for parameter correction of a fluorescence endoscope device, operating status judgment of a fluorescence endoscope device, and auxiliary adjustment of the operating status of the fluorescence endoscope device under different fluorescence modes.

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