Microscopic observation preparation method based on Gastrodia elata powder and its application

Abstract

The present invention discloses a microscopic observation preparation method based on Gastrodia elata powder and an application thereof, and relates to the technical field of Gastrodia elata powder preparation. The following scheme is proposed, comprising the following steps: S1, weighing: taking out an electronic scale, grabbing an appropriate amount of dry Gastrodia elata slices for weighing, S2, crushing: putting the weighed Gastrodia elata slices into a powder preparation device for crushing; the method performs microscopic observation on Gastrodia elata powder, and can identify and judge the quality of Gastrodia elata from various aspects, avoiding deviation in identification results caused by accidental or human factors, and ensuring the quality of Gastrodia elata. The method also assists in preparation through a heating mechanism, avoiding the situation where the solution is spilled or difficult to spread during heating, resulting in difficulty in permeabilization, and no manual hand-holding is required during permeabilization, saving time and effort, and avoiding the situation of artificial skin burns, thereby increasing the preparation speed, saving a lot of preparation time, improving the preparation efficiency of Gastrodia elata powder, and making identification more convenient.

Description

Technical Field

[0001] The present invention relates to the technical field of Gastrodia elata powder tableting, and in particular to a microscopic observation tableting method based on Gastrodia elata powder and an application thereof. Background Art

[0002] Gastrodia elata is one of the four most precious Chinese medicinal materials in my country. It has the effects of calming the liver, relieving wind and stopping spasms. Recent studies have found that Gastrodia elata also has the effects of increasing intelligence, strengthening the brain, treating Alzheimer's disease, delaying aging, anti-inflammatory, enhancing immune function, lowering blood pressure, adjusting heart rate, increasing cardiac output and other cardiovascular regulating effects, and its toxic side effects are very small.

[0003] The quality of cultivated Gastrodia elata and wild Gastrodia elata is different. Different quality means different nutritional value, resulting in a large price difference. Currently, most of the identification is done by manual observation of the appearance of wild Gastrodia elata, but the identification results are also subject to great randomness and human factors. For this reason, we proposed a microscopic observation preparation method based on Gastrodia elata powder and its application. Summary of the Invention

[0004] The present invention provides a microscopic observation preparation method based on Gastrodia elata powder and its application to solve the above-mentioned deficiencies in the prior art.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] The microscopic observation preparation method based on Gastrodia elata powder comprises the following steps:

[0007] S1. Weighing: Take out an electronic scale and grab an appropriate amount of dried Gastrodia elata slices for weighing;

[0008] S2, crushing: placing the weighed Gastrodia elata slices into a powder preparation device for crushing;

[0009] S3, screening: taking out the powder from the powder preparation device, and then putting it into the screening mechanism to screen the Gastrodia elata powder and remove large particles of powder;

[0010] S4. Clamping: Take out the glass slide, clamp an appropriate amount of powder and place it on the glass slide, then drop chloral hydrate solution onto the powder;

[0011] S5. Permeabilization: The slide is then placed on a permeabilization mechanism and heated for permeabilization. After permeabilization is complete, a coverslip is placed on the slide.

[0012] S6. Observation of the prepared powder: The permeabilized slide was then placed under a microscope for observation. The color of the powder was observed to be between yellow and brownish yellow. The shape of the thick-walled cells was observed to be oval or polygonal, with a diameter between 70 and 180 μm and a wall thickness between 3 and 8 μm. The cells were observed to be lignified and with obvious pits. The calcium oxalate raphe crystals were observed to be bundled or scattered, and with a length between 25 and 75 μm. After observation, the slide was removed.

[0013] S7, re-slide: then take out a new glass slide, and place the powder on the glass slide;

[0014] S8, adding glycerol acetate test solution: then add glycerol acetate test solution onto the powder, cover with a cover glass, and then place the slide under a microscope for observation;

[0015] S9. Microscopic observation of slides: Observe through a microscope to see whether the thin-walled cells containing the gelatinized polysaccharides are colorless, whether some cells have oblong, oblong or quasi-round particles, whether they appear brown or light brown-purple when exposed to iodine solution, and whether the diameters of the spiral ducts, reticulated ducts and annular ducts are in the range of 8 to 30 μm.

[0016] Furthermore, the powder preparation device in S2 includes a cylinder, a crushing knife, a cover and a dust reduction net. The dust reduction net is installed inside the cover. The crushing knife crushes the powder inside the cylinder. After the crushing is completed, the dust reduction net in the cover moves downward and penetrates into the cylinder to press down the flying dust in the cylinder. After the dust is reduced, the dust reduction net is reset. The powder preparation device reduces the dust of the powder after crushing to avoid the medicinal materials from being blown out when the cover is opened, causing waste.

[0017] Furthermore, the screening mechanism in S3 includes a double-layer pharmacopoeia sieve, the upper sieve aperture of the double-layer pharmacopoeia sieve is 0.25 mm, and the lower sieve aperture is 0.18 mm. The screening mechanism screens out large particles of powder through the double-layer pharmacopoeia sieve to avoid affecting tableting.

[0018] Furthermore, the permeabilization mechanism in S5 includes a base, a spring is fixed on the top of the base, a circular plate is fixed on the top of the spring, two support plates are fixed on the top of the circular plate, the top of the support plate is hinged with a pressure plate, and magnets are fixed on the side where the pressure plate and the support plate are close to each other, a cylinder is fixed on the top of the base, the outer rotation of the cylinder is connected to gear 1, a motor is fixed on the top of the base, one end of the output shaft of the motor is fixed with gear 2, one side of the gear 2 is meshed with gear 1, an L-shaped support frame is fixed on the top of the gear 1, a telescopic frame is fixed on the top of the L-shaped support frame, a fixing bolt is provided on the internal thread sleeve of one side of the telescopic frame, an inner rod is provided on the inner sleeve of the telescopic frame, a circular slide frame is fixed on the bottom of the circular plate, a ball is provided on the inner sleeve of the circular slide frame, and the bottom of the ball is hinged with the inner rod, and the permeabilization mechanism drives the slide to shake by the motor to spread the liquid, which is more stable and will not cause the solution to flow out of the slide due to excessive tilt angle, thereby avoiding hand fatigue and damage to the skin on the hands and saving labor time.

[0019] Furthermore, an alcohol cavity is provided inside the circular plate, a combustion core opening is provided on the top of the alcohol cavity, a combustion core is sleeved inside the combustion core opening, and the combustion core absorbs the alcohol in the alcohol cavity and burns to perform permeation.

[0020] Furthermore, the spring sleeve is arranged inside the cylinder, and a rubber pad is fixed to the bottom of the base, and the rubber pad reduces shock for the entire permeabilization mechanism.

[0021] Furthermore, the microscopic observation preparation method is used to calculate the wall-breaking rate of Gastrodia elata ultrafine powder.

[0022] Compared with the existing technology, the beneficial effects of the present invention are:

[0023] The method performs microscopic observation on slices of Gastrodia elata powder, and can identify and determine the quality of Gastrodia elata from all aspects, avoiding deviations in identification results caused by accidental or human factors, and ensuring the quality of Gastrodia elata. The method also uses a heating mechanism to assist in slice making, avoiding the situation where the solution is spilled or difficult to spread during slice making, resulting in difficulty in permeabilization. Moreover, no manual hand-holding is required during permeabilization, saving time and effort, and avoiding artificial skin burns. The slice making speed is increased, a large amount of slice making time is saved, the slice making efficiency of Gastrodia elata powder is improved, and identification is more convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The present invention provides a flowchart of a microscopic observation tablet preparation method based on Gastrodia elata powder;

[0025] Figure 2 This is a schematic diagram of the front top view of the three-dimensional structure of the permeabilization mechanism of the microscopic observation preparation method of Gastrodia elata powder proposed in the present invention;

[0026] Figure 3 This is a schematic diagram of the three-dimensional structure of the permeabilization mechanism of the microscopic observation preparation method of Gastrodia elata powder proposed in the present invention, viewed from the top right side;

[0027] Figure 4 This is a schematic diagram of the front view of the permeabilization mechanism of the microscopic observation preparation method based on Gastrodia elata powder proposed in the present invention;

[0028] Figure 5 This is a left-side structural schematic diagram of the permeabilization mechanism of the microscopic observation preparation method based on Gastrodia elata powder proposed in the present invention;

[0029] Figure 6 This is a schematic diagram of the three-dimensional structure of the permeabilization mechanism of the microscopic observation preparation method based on Gastrodia elata powder proposed in the present invention, viewed from the right front.

[0030] In the figure: 1. Base; 2. Spring; 3. Circular plate; 4. Combustion core port; 5. Combustion core; 6. Support plate; 7. Pressure plate; 8. Magnet; 9. Cylinder; 10. Gear 1; 11. L-shaped support frame; 12. Telescopic frame; 13. Inner rod; 14. Fixing bolt; 15. Circular slide frame; 16. Ball bearing; 17. Motor; 18. Gear 2. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0032] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limiting the present invention.

[0033] Example 1

[0034] Reference Figure 1-3 :The microscopic observation preparation method based on Gastrodia elata powder comprises the following steps:

[0035] S1. Weighing: Take out an electronic scale and grab an appropriate amount of dried Gastrodia elata slices for weighing;

[0036] S2, crushing: placing the weighed Gastrodia elata slices into a powder preparation device for crushing;

[0037] S3, screening: taking out the powder from the powder preparation device, and then putting it into the screening mechanism to screen the Gastrodia elata powder and remove large particles of powder;

[0038] S4. Clamping: Take out the glass slide, clamp an appropriate amount of powder and place it on the glass slide, then drop chloral hydrate solution onto the powder;

[0039] S5. Permeabilization: The slide is then placed on a permeabilization mechanism and heated for permeabilization. After permeabilization is complete, a coverslip is placed on the slide.

[0040] S6. Observation of the prepared powder: The permeabilized slide was then placed under a microscope for observation. The color of the powder was observed to be between yellow and brownish yellow. The shape of the thick-walled cells was observed to be oval or polygonal, with a diameter between 70 and 180 μm and a wall thickness between 3 and 8 μm. The cells were observed to be lignified and with obvious pits. The calcium oxalate raphe crystals were observed to be bundled or scattered, and with a length between 25 and 75 μm. After observation, the slide was removed.

[0041] S7, re-slide: then take out a new glass slide, and place the powder on the glass slide;

[0042] S8, adding glycerol acetate test solution: then add glycerol acetate test solution onto the powder, cover with a cover glass, and then place the slide under a microscope for observation;

[0043] S9. Microscopic observation of slides: Observe using a microscope to see if the thin-walled cells containing the gelatinized polysaccharide are colorless, if some cells have oblong, elliptical, or quasi-round particles, if they appear brown or light brown-purple when exposed to iodine solution, and if the diameters of the spiral, reticulate, and annular ducts are between 8 and 30 μm.

[0044] The powder preparation device in S2 includes a cylinder, a crushing knife, a cover and a dust suppression net. The dust suppression net is installed inside the cover. The crushing knife crushes the powder inside the cylinder. After the crushing is completed, the dust suppression net in the cover moves downward and penetrates into the cylinder to press down the flying dust in the cylinder. After the dust is suppressed, the dust suppression net is reset. The powder preparation device suppresses the powder after crushing to avoid the waste of medicinal materials when the cover is opened. The screening mechanism in S3 includes a double-layer pharmacopoeia sieve. The aperture of the upper screen of the double-layer pharmacopoeia sieve is 0.25mm, and the aperture of the lower screen is 0.18mm. The screening mechanism screens out large particles of powder through the double-layer pharmacopoeia sieve to avoid affecting tableting;

[0045] The permeation mechanism in S5 includes a base 1, a spring 2 is fixed on the top of the base 1, a circular plate 3 is fixed on the top of the spring 2, two support plates 6 are fixed on the top of the circular plate 3, a pressure plate 7 is hinged on the top of the support plate 6, a magnet 8 is fixed on the side where the pressure plate 7 and the support plate 6 are close to each other, a cylinder 9 is fixed on the top of the base 1, the outer rotation of the cylinder 9 is connected to the gear 10, a motor 17 is fixed on the top of the base 1, a gear 2 18 is fixed on one end of the output shaft of the motor 17, one side of the gear 2 18 is meshed with the gear 10, and the top of the gear 10 is fixed. An L-shaped support frame 11 is fixed with a telescopic frame 12 on the top of the L-shaped support frame 11, and a fixing bolt 14 is provided on the internal threaded sleeve of one side of the telescopic frame 12. An inner sleeve of the telescopic frame 12 is provided with an inner rod 13, and a circular slide frame 15 is fixed to the bottom of the circular plate 3. A ball 16 is provided inside the circular slide frame 15, and the bottom of the ball 16 is hinged to the inner rod 13. The permeabilization mechanism drives the slide to shake through the motor 17 to spread the liquid, which is more stable and will not cause the solution to flow out of the slide due to excessive tilt angle, thereby avoiding hand fatigue and damage to the skin on the hands and saving labor time.

[0046] Example 2

[0047] Reference Figure 2-6 This embodiment provides a technical solution based on the first embodiment: an alcohol chamber is opened inside the circular plate 3, a combustion core opening 4 is opened on the top of the alcohol chamber, a combustion core 5 is set inside the combustion core opening 4, and the combustion core 5 absorbs the alcohol in the alcohol chamber and burns to perform permeation;

[0048] The spring 2 is sleeved inside the cylinder 9, and a rubber pad is fixed to the bottom of the base 1. The rubber pad reduces shock for the entire permeabilization mechanism. Application of microscopic observation preparation method in calculating the wall-breaking rate of Gastrodia elata ultrafine powder.

[0049] The working principle of the permeabilization mechanism: first, ignite the combustion core, rotate the pressure plate 7 around the hinge point, then place the glass slide to be heated on the support plate 6 and reset the pressure plate 7. The two magnets 8 attract each other to reset the glass slide, then loosen the fixing bolt 14, retract a part of the inner rod 13 into the telescopic frame 12, and then tighten the fixing bolt 14 to limit it. At this time, the circular plate 3 and the glass slide are in an inclined state, and then start the motor 17 to drive the gear 2 18, gear 1 10, cylinder 9, L-shaped support frame 11, inner rod 13 and ball 16 and other structures to rotate. The ball 16 moves inside the circular slide frame 15, so that the inclined position of the circular plate 3 keeps changing, so that the solution on the glass slide will gradually spread out, accelerating the permeabilization. The advantage of the permeabilization mechanism over manual hand-held permeabilization is that it is more stable, will not cause the solution to flow out of the glass slide due to excessive tilt angle, avoids hand fatigue and damage to the skin on the hands, and saves labor time.

[0050] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A microscopic observation preparation method based on Gastrodia elata powder, characterized in that: The following steps are involved: S1. Weighing: Take out an electronic scale and grab an appropriate amount of dried Gastrodia elata slices for weighing; S2, crushing: placing the weighed Gastrodia elata slices into a powder preparation device for crushing; S3, screening: then taking out the powder from the powder preparation device, and then putting it into the screening mechanism to screen the Gastrodia elata powder and remove large particles of powder; S4. Clamping: Then take out the glass slide, clamp an appropriate amount of powder and place it on the glass slide, and then drop the chloral hydrate solution onto the powder; S5. Permeabilization: The slide is then placed on a permeabilization mechanism and heated for permeabilization. After permeabilization is complete, a coverslip is placed on the slide. S6. Observation of the prepared powder: The permeabilized slide was then placed under a microscope for observation. The color of the powder was observed to be between yellow and brownish yellow. The shape of the thick-walled cells was observed to be oval or polygonal, the diameter was between 70 and 180 μm, the wall thickness was between 3 and 8 μm, and the cells were lignified. The pits were obvious. The calcium oxalate raphe crystals were observed to be bundled or scattered, and the length was between 25 and 75 μm. The observed data were recorded and the slide was removed after observation. S7, re-slide: then take out a new glass slide, and clamp the powder and place it on the glass slide; S8, adding glycerol acetate test solution: then add glycerol acetate test solution dropwise onto the powder, cover with a cover glass, and then place the slide under a microscope for observation; S9. Microscopic observation of slides: Finally, observe under a microscope whether the thin-walled cells containing the gelatinized polysaccharide are colorless, whether some cells have oblong, oblong, or quasi-round particles, whether they appear brown or light brown-purple when exposed to iodine solution, and whether the diameters of the spiral vessels, reticulated vessels, and annular vessels are in the range of 8-30 μm. Then, combine the observation data in S6 to determine the quality of the Gastrodia elata. The powder preparation device in S2 includes a cylinder, a crushing knife, a cover, and a dust suppression net. The dust suppression net is installed inside the cover. The crushing knife crushes the powder inside the cylinder. After the crushing is completed, the dust suppression net in the cover moves downward and penetrates into the cylinder to press down the flying dust in the cylinder. After the dust is suppressed, the dust suppression net is reset. The screening mechanism in S3 includes a double-layer pharmacopoeia sieve, wherein the aperture of the upper layer of the double-layer pharmacopoeia sieve is 0.25 mm, and the aperture of the lower layer of the sieve is 0.18 mm; The permeation mechanism in S5 comprises a base (1), a spring (2) is fixed on the top of the base (1), a circular plate (3) is fixed on the top of the spring (2), two support plates (6) are fixed on the top of the circular plate (3), a pressure plate (7) is hinged on the top of the support plate (6), a magnet (8) is fixed on the side where the pressure plate (7) and the support plate (6) are close to each other, a cylinder (9) is fixed on the top of the base (1), the outer rotation of the cylinder (9) is connected to a gear (10), a motor (17) is fixed on the top of the base (1), and the output shaft of the motor (17) is A gear 2 (18) is fixed at the end, one side of the gear 2 (18) is meshed with the gear 1 (10), an L-shaped support frame (11) is fixed to the top of the gear 1 (10), a telescopic frame (12) is fixed to the top of the L-shaped support frame (11), a fixing bolt (14) is provided on the internal thread sleeve of one side of the telescopic frame (12), an inner sleeve of the telescopic frame (12) is provided with an inner rod (13), a circular slide frame (15) is fixed to the bottom of the circular plate (3), a ball (16) is provided on the internal sleeve of the circular slide frame (15), and the bottom of the ball (16) is hinged to the inner rod (13); An alcohol cavity is provided inside the circular plate (3), a combustion core opening (4) is provided on the top of the alcohol cavity, and a combustion core (5) is sleeved inside the combustion core opening (4).

2. The microscopic observation preparation method based on Gastrodia elata powder according to claim 1, characterized in that: The spring (2) is sleeved inside the cylinder (9), and a rubber pad is fixed to the bottom of the base (1).

3. according to the application of the microscopic observation film-making method described in any one of claim 1-2 in calculating the Gastrodia elata superfine powder wall-breaking rate.

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