A microbial treatment agent for garbage leachate and preparation method thereof

Abstract

The present invention discloses a microbial treatment agent for landfill leachate and a preparation method thereof. The treatment agent comprises a support layer and microbial agent particles on its surface, wherein the support layer is made of a carbon layer and a modified resin, and the microbial agent particles are made of a microbial agent and a microbial agent matrix. The present invention forms the support layer and the microbial agent by setting the treatment agent components and preparation methods. The support layer is a spherical support layer made of a carbon layer and a modified resin. The carbon layer is made of a combination of sheet-like skeletons and is wrapped by the modified resin to reduce pores. The sheet-like skeletons are located in different planes. Because the carbon layer and the modified resin have low density and hydrophobic properties, when the treatment agent is impacted by wind or water flow, the treatment agent rolls on the surface of the leachate. After contacting the leachate, the microbial agent can come into contact with air, thereby enhancing the energy metabolism of aerobic bacteria in the microbial agent and improving the treatment efficiency of the treatment agent.

Description

Technical Field

[0001] The present invention relates to the technical field of garbage treatment, in particular to a microbial treatment agent for garbage leachate and a preparation method thereof. Background Art

[0002] We call discarded items that have lost their usefulness and cannot be used garbage. These materials, including unwanted or useless solid and fluid substances, are a crucial part of the material cycle. With the advancement of society and the growing awareness of energy conservation and environmental protection, the concept of "garbage is a misplaced resource" has become increasingly popular, emphasizing the value of recycling. In addition to recyclable garbage, non-recyclable waste also requires treatment. Common treatment methods include incineration or landfill. During the stacking and landfilling process, garbage undergoes biochemical degradation through compaction and fermentation, while seepage from precipitation and groundwater produces highly concentrated liquids containing organic and inorganic components. These liquids cannot be directly discharged and require further treatment. Conventional treatment methods include traditional activated sludge processes, stabilization ponds, bio-rotating discs, and anaerobic fixed-film bioreactors. Traditional activated sludge processes, stabilization ponds, and bio-rotating discs are aerobic treatment processes, while anaerobic fixed-film bioreactors are anaerobic processes. These processes generally suffer from long treatment times, high investment costs, low pollutant removal rates, and unpleasant odors. Therefore, we propose a microbial treatment agent for landfill leachate and a preparation method. Summary of the Invention

[0003] The object of the present invention is to provide a microbial treatment agent for landfill leachate and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0004] In order to solve the above technical problems, the present invention provides the following technical solutions: a microbial treatment agent for landfill leachate, the treatment agent comprising a support layer and microbial agent particles on its surface, the support layer being made of a carbon layer and a modified resin, and the microbial agent particles being made of a microbial agent and a microbial agent matrix.

[0005] Furthermore, the modified resin is prepared from the following components by weight: 0.5 to 1 parts of agar, 10 to 21 parts of acrylic acid monomer, 0.2 to 1.3 parts of photoinitiator, 8 to 12 parts of polyvinyl alcohol, 3 to 18 parts of dicyclohexylcarbodiimide, and 0.1 to 0.5 parts of 4-dimethylaminopyridine.

[0006] Furthermore, the bacterial agent matrix is ​​prepared from the following components by weight: 15.8 to 17.2 parts of acrylic acid monomer and 1 to 2 parts of agar.

[0007] In the above technical solution, the acrylic acid monomer is a mixture of acrylic acid monomer, styrene, and butyl methacrylate monomer.

[0008] Furthermore, the carbon layer is a spherical carbon layer made from a flaky skeleton.

[0009] A method for preparing a microbial treatment agent for landfill leachate comprises the following steps:

[0010] (1) Preparation of the scaffold layer: dry carbonize the carbon source and mix and react with the remaining materials to obtain the scaffold layer;

[0011] (2) Preparation of microbial agent: taking materials and blending them to prepare microbial agent;

[0012] (3) Preparation of treatment agent: Mix the support layer with the bacterial agent and expose to light to obtain the treatment agent.

[0013] Furthermore, the step (1) includes the following steps:

[0014] The carbon source is crushed and dehydrated, spread on a concave-convex plate with honeycomb protrusions, pressed and dried, and then crushed to obtain a sheet-like carbon layer with a diameter of 3 to 10 mm;

[0015] The flaky carbon layer is mixed with a starch aqueous solution, stirred at 80-95°C for 1-3 hours, and carbonized at high temperature in an argon atmosphere to obtain a spherical carbon frame;

[0016] The spherical carbon frame is treated with a mixed acid solution of concentrated sulfuric acid and concentrated nitric acid, and then placed at a constant temperature of 88 to 92°C for 150 to 200 minutes, and the product is dried to form a carbon layer with a flaky skeleton;

[0017] Add deionized water to agar, heat to 75-80°C, stir and dissolve, add acrylic acid monomer and photoinitiator, cool in the dark, and react under light at room temperature for 200-280 minutes to prepare modified polyacrylic acid;

[0018] Take polyvinyl alcohol and add deionized water, heat it to 90-94°C and stir to dissolve, cool it to 68-70°C, add carbon layer, dicyclohexylcarbodiimide, and 4-dimethylaminopyridine in sequence and mix them evenly, keep the temperature constant for 12-24 hours, maintain the system temperature, add modified polyacrylic acid and stir to mix, take the mixed spherical product and freeze-dry it to form a carbon layer wrapped by the modified resin to prepare the scaffold layer.

[0019] Furthermore, the step (2) includes the following steps:

[0020] Dissolving acrylic acid monomer in a solvent, adding a photoinitiator to prepare a solution;

[0021] Take the microbial agent and add it into deionized water, stir and disperse it to prepare a suspension;

[0022] Add agar to deionized water, heat to 75-80°C, stir and dissolve, cool to 38-42°C, add the suspension and stir thoroughly, add dropwise to a solution with a temperature of 20-35°C, let stand for 20-30 minutes to form an agent matrix wrapped with the microbial agent, and prepare the agent.

[0023] Furthermore, the step (3) includes the following steps:

[0024] The scaffold layer and the bacterial agent were mixed evenly, placed under light conditions, and slowly stirred at room temperature for 200 to 280 minutes to prepare a treatment agent.

[0025] Furthermore, the density of the carbon layer in step (1) is 0.5 to 0.8 g / cm³.

[0026] Furthermore, the carbonization process of step (1) is as follows: under a pressure of -0.1 to 0 MPa, the temperature is raised from room temperature to 800 to 900°C at a heating rate of 10°C / min, and the temperature is kept constant for 1 to 2 hours.

[0027] In the above technical solution, the carbon source is selected from inedible plants discarded from farmers' markets and planting bases and their roots, stems, leaves, etc.; after carbonization, the plants have a certain amount of orderly veins, which is conducive to enhancing the structure of the carbon layer and its adsorption performance, and is conducive to the proliferation and loading of bacteria; a sheet carbon layer is prepared by the process in step (1), and then a spherical carbon frame is formed by combining the sheet skeleton, and the carbon layer is obtained by carbonization, which has porous properties and a high specific surface area; polyvinyl alcohol is first reacted with the carbon layer formed after acidification with a mixed acid solution, and then dicyclohexylcarbodiimide and 4-dimethylaminopyridine are added to react with each other, so that the surface of the carbon layer is porous. The carbon layer is coated with functional groups such as polyurethane, esters, amino groups, and ester groups to form an insoluble polymer outside the carbon layer, thereby increasing the hydrophobicity of the scaffold layer and promoting the absorption of carbon dioxide produced by bacteria. The carbon layer is then wrapped with a modified acrylic monomer prepared by mixing the resulting reaction product with agar and acrylic monomer, thereby improving the mechanical strength of the scaffold layer while providing good elasticity and dimensional stability. A stable porous structure is formed by repeated freezing cycles. Due to the presence of a flaky skeleton, the prepared modified resin is spread flat on the surface of the flaky skeleton, further improving the immobilization stability of the treatment agent and making the immobilized microorganisms more active.

[0028] In step (2), agar is used as a culture medium for the microbial agent and is wrapped by an acrylic acid monomer ester monomer solution to form a core-shell structure to prepare the agent; in step (3), the acrylic acid monomer ester in the shell of the agent and the acrylic acid monomer on the surface of the support layer are polymerized under the action of light to fix the agent on the surface of the support layer to prepare the treatment agent.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] 1. The present invention provides a microbial treatment agent for landfill leachate and a preparation method thereof. By setting the treatment agent components and the preparation method, a support layer and a microbial agent are formed, wherein the support layer is a spherical support layer made of a carbon layer and a modified resin, and the carbon layer is made by carbonizing a combination of flaky carbon layers and is wrapped by the modified resin to block pores. Since the carbon layer is made of a flaky carbon layer, the surface of the spherical support layer is uneven. Since the carbon layer and the modified resin have a low density and hydrophobic properties, the treatment agent is more easily deflected on the leachate surface by wind or water impact. After contacting the leachate, the microbial agent can contact the air, thereby improving the energy metabolism of aerobic bacteria in the microbial agent and improving the treatment efficiency of the treatment agent. The microbial agent is wrapped by a microbial agent matrix, and while being resistant to leachate erosion, has good mechanical properties and gloss, can reflect light to promote the fixation of the microbial agent to the support layer, and improve the stability of the microbial agent.

[0031] 2. The microbial treatment agent for landfill leachate of the present invention and its preparation method form a support layer and a microbial agent by setting the treatment agent components and preparation methods, wherein the support layer includes a carbon layer and a modified resin wrapped on its surface, and the microbial agent includes a microbial agent and a microbial agent matrix. The microbial agent utilizes the microbial agent matrix on the surface to connect with the modified resin on the surface of the support layer, and finally fixes the microbial agent on the surface of the support layer to form a treatment agent structure; because the components in the support layer and the microbial agent are conducive to the growth and reproduction of microorganisms, the modified resin in the support layer wraps the carbon layer, reducing the pore size of the prepared support layer, and can prevent bacteria from entering the interior of the treatment agent in the early stage of use, promote the reproduction and fixation of bacteria in the microbial agent on the surface of the support layer, increase the number of bacteria in the treatment agent, and improve the treatment efficiency of the treatment agent for leachate; at the same time, it prevents bacteria from reproducing and moving inside the treatment agent, expands the pores to allow water to enter, causing the treatment agent to sink in the leachate, and reduces the activity of aerobic bacteria.

[0032] 3. The microbial treatment agent for garbage leachate and the preparation method of the present invention form a scaffold layer and a microbial agent fixed on its surface by setting the treatment agent components and the preparation method. The modified resin in the scaffold layer can absorb heavy metal ions and organic matter in the leachate, and can absorb the decomposition enzymes produced by the microbial agent, etc., contact with the adsorbed organic matter and absorb and degrade it, thereby achieving the synchronous degradation of heavy metal ions and organic matter; at the same time, heavy metal ions enter the treatment agent structure, which can strengthen the modified resin therein, improve the sustained mechanical properties of the treatment agent, and prevent the treatment agent from swelling and breaking in the leachate and affecting bacterial reproduction.

[0033] 4. The microbial treatment agent for landfill leachate and the preparation method of the present invention, by setting the treatment agent components and preparation method, in the initial use, due to the low density of the center of the support layer, the prepared treatment agent is suspended on the surface of the leachate and deflected by the action of wind and water. The microbial agent in the treatment agent can contact the leachate and then contact the air, which is convenient for the full reproduction and metabolism of the bacteria in the microbial agent; as the treatment agent is used, the bacteria proliferate along its outer surface, improving the treatment effect; the agar in the component is biodegradable, and the treatment agent gradually produces pores, which is convenient for the proliferation and metabolism of anaerobic bacteria in the microbial agent, and at the same time, the modified resin absorbs heavy metal ions and organic matter in the leachate. , working together to treat the pollutants in the leachate; the modified resin absorbs the carbon dioxide produced by the degradation of organic matter and the volatile substances emitted by the leachate, preventing the reduction of the oxygen content on the leachate surface and preventing the emission of odor; as the pores increase, water flows in, the treatment agent gradually sinks, the action of anaerobic bacteria in the agent is enhanced, and the degradation of suspended organic matter is promoted; as the degradable components in the treatment agent decompose, the carbon layer is exposed, and water is adsorbed and introduced, causing the treatment agent to sink to the bottom of the leachate, and adsorb and fix the pollutants at the bottom, thus completing the use process of the treatment agent, and the products after the use of the treatment agent can be recovered and treated to realize the reuse of the treatment agent. DETAILED DESCRIPTION

[0034] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0035] Example 1

[0036] (1) Preparation of scaffold layer:

[0037] The carbon source is crushed and dehydrated, spread on a concave-convex plate with honeycomb protrusions, pressed and dried, and then crushed to obtain a flaky carbon layer with a diameter of 3 to 10 mm. The flaky carbon layer is mixed with an aqueous solution of starch, stirred at 80°C for 1 hour, and carbonized at high temperature in an argon atmosphere to obtain a spherical carbon frame. The carbonization process is as follows: under a pressure of 0 MPa, the heating rate is increased from room temperature to 800°C at a constant temperature of 10°C / min, and the spherical carbon frame is obtained after being kept at this temperature for 1 hour.

[0038] The spherical carbon frame is treated with a mixed acid solution of concentrated sulfuric acid and concentrated nitric acid, and then placed at a constant temperature of 88°C for 150 hours. The product is dried to form a carbon layer with a flaky skeleton.

[0039] 0.7 parts of agar were added to deionized water, heated to 75°C and stirred to dissolve, 15 parts of acrylic acid monomer and 0.7 parts of photoinitiator were added, cooled in the dark, and then placed under light for reaction at room temperature for 200 minutes to obtain modified polyacrylic acid;

[0040] Take 10 parts of polyvinyl alcohol and add deionized water, heat to 90°C and stir to dissolve, cool to 68°C, add carbon layer, 10 parts of dicyclohexylcarbodiimide, and 0.3 parts of 4-dimethylaminopyridine in sequence and mix evenly, keep the temperature constant for 12 hours, maintain the system temperature, add modified polyacrylic acid and stir to mix, take the mixed spherical product and freeze-dry to form a carbon layer wrapped by the modified resin to prepare the scaffold layer.

[0041] (2) Preparation of microbial agents:

[0042] 16.5 parts of acrylic acid monomer was dissolved in a solvent, and a photoinitiator was added to prepare a solution;

[0043] Take the microbial agent and add it into deionized water, stir and disperse it to prepare a suspension;

[0044] Take 1.5 parts of agar and add it to deionized water, heat it to 75°C and stir to dissolve, cool it to 38°C, add the suspension and stir it thoroughly, add it dropwise to the solution at a temperature of 20°C, let it stand for 20 minutes to form a microbial agent matrix wrapped with the microbial agent, and prepare the microbial agent.

[0045] (3) Preparation of treatment agent:

[0046] The scaffold layer was mixed evenly with the bacterial agent, placed under light conditions, and slowly stirred at room temperature for 200 minutes to prepare a treatment agent.

[0047] Example 2

[0048] (1) Preparation of scaffold layer:

[0049] The carbon source was crushed and dehydrated, spread on a concave-convex plate with honeycomb protrusions, pressed and dried, and then crushed to obtain a flaky carbon layer with a diameter of 3 to 10 mm. The flaky carbon layer was mixed with an aqueous solution of starch, stirred at 87°C for 2 hours, and carbonized at high temperature in an argon atmosphere to obtain a spherical carbon frame. The carbonization process was as follows: under a pressure of -0.05 MPa, the heating rate was increased from room temperature to 850°C at a rate of 10°C / min, and the spherical carbon frame was obtained after constant temperature treatment for 1.5 hours.

[0050] The spherical carbon frame is treated with a mixed acid solution of concentrated sulfuric acid and concentrated nitric acid, and then placed at a constant temperature of 90°C for 180 hours. The product is dried to form a carbon layer with a flaky skeleton.

[0051] Take 0.7 parts of agar and add deionized water, heat to 77°C and stir to dissolve, add 15 parts of acrylic acid monomer and 0.7 parts of photoinitiator, cool in the dark, and place under light to react at room temperature for 240 minutes to prepare modified polyacrylic acid;

[0052] Take 10 parts of polyvinyl alcohol and add deionized water, heat to 92°C and stir to dissolve, cool to 69°C, add carbon layer, 10 parts of dicyclohexylcarbodiimide, and 0.3 parts of 4-dimethylaminopyridine in sequence and mix evenly, keep the temperature constant for 18 hours, maintain the system temperature, add modified polyacrylic acid and stir to mix, take the mixed spherical product and freeze-dry to form a carbon layer wrapped by modified resin to prepare the scaffold layer.

[0053] (2) Preparation of microbial agents:

[0054] 16.5 parts of acrylic acid monomer was dissolved in a solvent, and a photoinitiator was added to prepare a solution;

[0055] Take the microbial agent and add it into deionized water, stir and disperse it to prepare a suspension;

[0056] Take 1.5 parts of agar and add it to deionized water, heat it to 77°C and stir to dissolve, cool it to 40°C, add the suspension and stir it thoroughly, add it dropwise to the solution at a temperature of 27°C, let it stand for 25 minutes to form a microbial agent matrix wrapped with the microbial agent, and prepare the microbial agent.

[0057] (3) Preparation of treatment agent:

[0058] The scaffold layer was mixed evenly with the bacterial agent, placed under light conditions, and slowly stirred at room temperature for 240 minutes to prepare a treatment agent.

[0059] Example 3

[0060] (1) Preparation of scaffold layer:

[0061] The carbon source is crushed and dehydrated, spread on a concave-convex plate with honeycomb protrusions, pressed and dried, and then crushed to obtain a flaky carbon layer with a diameter of 3 to 10 mm. The flaky carbon layer is mixed with an aqueous solution of starch, stirred at 95°C for 3 hours, and carbonized at high temperature in an argon atmosphere to obtain a spherical carbon frame. The carbonization process is as follows: under a pressure of -0.1 MPa, the heating rate is increased from room temperature to 900°C at a rate of 10°C / min, and the spherical carbon frame is obtained after constant temperature treatment for 2 hours.

[0062] The spherical carbon frame was treated with a mixed acid solution of concentrated sulfuric acid and concentrated nitric acid, and then placed at 92°C for a constant temperature reaction for 200 hours. The product was dried to form a carbon layer with a flaky skeleton.

[0063] Take 0.7 parts of agar and add deionized water, heat to 80°C and stir to dissolve, add 15 parts of acrylic acid monomer and 0.7 parts of photoinitiator, cool in the dark, and place under light to react at room temperature for 240 minutes to prepare modified polyacrylic acid;

[0064] Take 10 parts of polyvinyl alcohol and add deionized water, heat to 94°C and stir to dissolve, cool to 70°C, add carbon layer, 10 parts of dicyclohexylcarbodiimide, and 0.3 parts of 4-dimethylaminopyridine in sequence and mix evenly, keep the temperature constant for 24 hours, maintain the system temperature, add modified polyacrylic acid and stir to mix, take the mixed spherical product and freeze-dry to form a carbon layer wrapped by modified resin to prepare the scaffold layer.

[0065] (2) Preparation of microbial agents:

[0066] 16.5 parts of acrylic acid monomer was dissolved in a solvent, and a photoinitiator was added to prepare a solution;

[0067] Take the microbial agent and add it into deionized water, stir and disperse it to prepare a suspension;

[0068] Take 1.5 parts of agar and add it to deionized water, heat it to 80°C and stir to dissolve, cool it to 42°C, add the suspension and stir it thoroughly, add it dropwise to the solution at a temperature of 35°C, let it stand for 30 minutes to form a microbial agent matrix wrapped with the microbial agent, and prepare the microbial agent.

[0069] (3) Preparation of treatment agent:

[0070] The scaffold layer was mixed evenly with the bacterial agent, placed under light conditions, and slowly stirred at room temperature for 280 minutes to prepare a treatment agent.

[0071] Example 4

[0072] (1) Preparation of scaffold layer:

[0073] The carbon source was crushed and dehydrated, spread on a concave-convex plate with honeycomb protrusions, pressed and dried, and then crushed to obtain a flaky carbon layer with a diameter of 3 to 10 mm. The flaky carbon layer was mixed with an aqueous solution of starch, stirred at 87°C for 2 hours, and carbonized at high temperature in an argon atmosphere to obtain a spherical carbon frame. The carbonization process was as follows: under a pressure of -0.05 MPa, the heating rate was increased from room temperature to 850°C at a rate of 10°C / min, and the spherical carbon frame was obtained after constant temperature treatment for 1.5 hours.

[0074] The spherical carbon frame is treated with a mixed acid solution of concentrated sulfuric acid and concentrated nitric acid, and then placed at a constant temperature of 90°C for 180 hours. The product is dried to form a carbon layer with a flaky skeleton.

[0075] Take 0.5 parts of agar and add deionized water, heat to 77°C and stir to dissolve, add 10 parts of acrylic acid monomer and 0.2 parts of photoinitiator, cool in the dark, and place under light to react at room temperature for 240 minutes to prepare modified polyacrylic acid;

[0076] Take 8 parts of polyvinyl alcohol and add deionized water, heat to 92°C and stir to dissolve, cool to 69°C, add carbon layer, 3 parts of dicyclohexylcarbodiimide, and 0.1 parts of 4-dimethylaminopyridine in sequence and mix evenly, keep the temperature constant for 18 hours, maintain the system temperature, add modified polyacrylic acid and stir to mix, take the mixed spherical product and freeze-dry to form a carbon layer wrapped by the modified resin to prepare the scaffold layer.

[0077] (2) Preparation of microbial agents:

[0078] 15.8 parts of acrylic acid monomer was dissolved in a solvent, and a photoinitiator was added to prepare a solution;

[0079] Take the microbial agent and add it into deionized water, stir and disperse it to prepare a suspension;

[0080] Take 1 part of agar and add it to deionized water, heat it to 77°C and stir to dissolve, cool it to 40°C, add the suspension and stir it thoroughly, add it dropwise to the solution at a temperature of 27°C, let it stand for 25 minutes to form a microbial agent matrix wrapped with the microbial agent, and prepare the microbial agent.

[0081] (3) Preparation of treatment agent:

[0082] The scaffold layer was mixed evenly with the bacterial agent, placed under light conditions, and slowly stirred at room temperature for 240 minutes to prepare a treatment agent.

[0083] Example 5

[0084] (1) Preparation of scaffold layer:

[0085] The carbon source was crushed and dehydrated, spread on a concave-convex plate with honeycomb protrusions, pressed and dried, and then crushed to obtain a flaky carbon layer with a diameter of 3 to 10 mm. The flaky carbon layer was mixed with an aqueous solution of starch, stirred at 87°C for 2 hours, and carbonized at high temperature in an argon atmosphere to obtain a spherical carbon frame. The carbonization process was as follows: under a pressure of -0.05 MPa, the heating rate was increased from room temperature to 850°C at a rate of 10°C / min, and the spherical carbon frame was obtained after constant temperature treatment for 1.5 hours.

[0086] The spherical carbon frame is treated with a mixed acid solution of concentrated sulfuric acid and concentrated nitric acid, and then placed at a constant temperature of 90°C for 180 hours. The product is dried to form a carbon layer with a flaky skeleton.

[0087] Take 1 part of agar and add it to deionized water, heat it to 77℃ and stir to dissolve it, add 21 parts of acrylic acid monomer and 1.3 parts of photoinitiator, cool it in the dark, and then place it under light to react at room temperature for 240 minutes to prepare modified polyacrylic acid;

[0088] Take 12 parts of polyvinyl alcohol and add deionized water, heat to 92°C and stir to dissolve, cool to 69°C, add carbon layer, 18 parts of dicyclohexylcarbodiimide, and 0.5 parts of 4-dimethylaminopyridine in sequence and mix evenly, keep the temperature constant for 18 hours, maintain the system temperature, add modified polyacrylic acid and stir to mix, take the mixed spherical product and freeze-dry to form a carbon layer wrapped by the modified resin to prepare the scaffold layer.

[0089] (2) Preparation of microbial agents:

[0090] 17.2 parts of acrylic acid monomer was dissolved in a solvent, and a photoinitiator was added to prepare a solution;

[0091] Take the microbial agent and add it into deionized water, stir and disperse it to prepare a suspension;

[0092] Take 2 parts of agar and add deionized water, heat to 77°C and stir to dissolve, cool to 40°C, add the suspension and stir thoroughly, add dropwise to the solution at a temperature of 27°C, let it stand for 25 minutes to form a microbial agent matrix wrapped with the microbial agent, and prepare the microbial agent.

[0093] (3) Preparation of treatment agent:

[0094] The scaffold layer was mixed evenly with the bacterial agent, placed under light conditions, and slowly stirred at room temperature for 240 minutes to prepare a treatment agent.

[0095] Comparative Example 1

[0096] Step (1) Preparation of the scaffold layer:

[0097] The carbon source was crushed and dehydrated, spread on a concave-convex plate with honeycomb protrusions, pressed and dried, and then crushed to obtain a flaky carbon layer with a diameter of 3 to 10 mm. The flaky carbon layer was mixed with an aqueous solution of starch, stirred at 87°C for 2 hours, and carbonized at high temperature in an argon atmosphere to obtain a spherical carbon frame. The carbonization process was as follows: under a pressure of -0.05 MPa, the heating rate was increased from room temperature to 850°C at a rate of 10°C / min, and the spherical carbon frame was obtained after constant temperature treatment for 1.5 hours.

[0098] The remaining steps and implementation parameters are the same as those in Example 2.

[0099] Comparative Example 2

[0100] Step (1) Preparation of the scaffold layer:

[0101] The carbon source is crushed and dehydrated, spread on a concave-convex plate with honeycomb protrusions, pressed and dried, and then crushed to obtain a flaky carbon layer with a diameter of 3 to 10 mm. The flaky carbon layer is mixed with an aqueous solution of starch, stirred at 87°C for 2 hours, and carbonized at high temperature in an argon atmosphere to obtain a spherical carbon frame. The carbonization process is as follows: under a pressure of -0.05 MPa, the heating rate is increased from room temperature to 850°C at a rate of 10°C / min. After constant temperature treatment for 1.5 hours, a spherical carbon frame is obtained, forming a carbon layer with a flaky skeleton;

[0102] Take 0.7 parts of agar and add deionized water, heat to 77°C and stir to dissolve, add 15 parts of acrylic acid monomer and 0.7 parts of photoinitiator, cool in the dark, and place under light to react at room temperature for 240 minutes to prepare modified polyacrylic acid;

[0103] Take 10 parts of polyvinyl alcohol and add it to deionized water, heat it to 92°C and stir to dissolve, cool it to 69°C and add the carbon layer and mix evenly, keep the temperature constant for 18 hours, maintain the system temperature, add modified polyacrylic acid and stir to mix, take the mixed spherical product and freeze-dry it to form a carbon layer wrapped by the modified resin to prepare the scaffold layer.

[0104] The remaining steps and implementation parameters are the same as those in Example 2.

[0105] Comparative Example 3

[0106] (1) Preparation of scaffold layer:

[0107] The carbon source is crushed and dehydrated, spread on a concave-convex plate with honeycomb protrusions, pressed and dried, and then crushed to obtain a flaky carbon layer with a diameter of 3 to 10 mm. The flaky carbon layer is mixed with an aqueous solution of starch, stirred at 87°C for 2 hours, and carbonized at high temperature in an argon atmosphere to obtain a spherical carbon frame. The carbonization process is as follows: under a pressure of -0.05 MPa, the heating rate is increased from room temperature to 850°C at a rate of 10°C / min. After constant temperature treatment for 1.5 hours, a spherical carbon frame is obtained, forming a carbon layer with a flaky skeleton;

[0108] Take 0.7 parts of agar and add deionized water, heat to 77°C and stir to dissolve, add 15 parts of acrylic acid monomer and 0.7 parts of photoinitiator, cool in the dark, and place under light to react at room temperature for 240 minutes to prepare modified polyacrylic acid;

[0109] The carbon layer is added with modified polyacrylic acid and mixed to form a carbon layer wrapped by the modified resin to prepare the stent layer.

[0110] (2) Preparation of microbial agents:

[0111] 16.5 parts of acrylic acid monomer was dissolved in a solvent, and a photoinitiator was added to prepare a solution;

[0112] Take the microbial agent and add it into deionized water, stir and disperse it to prepare a suspension;

[0113] Take 1.5 parts of agar and add it to deionized water, heat it to 77°C and stir to dissolve, cool it to 40°C, add the suspension and stir it thoroughly, add it dropwise to the solution at a temperature of 27°C, let it stand for 25 minutes to form a microbial agent matrix wrapped with the microbial agent, and prepare the microbial agent.

[0114] (3) Preparation of treatment agent:

[0115] The scaffold layer was mixed evenly with the bacterial agent, and slowly stirred at room temperature for 240 minutes to prepare the treatment agent.

[0116] Comparative Example 4

[0117] (1) Preparation of scaffold layer:

[0118] Take 0.7 parts of agar and add deionized water, heat to 77°C and stir to dissolve, add 15 parts of acrylic acid monomer and 0.7 parts of photoinitiator, cool in the dark, and place under light to react at room temperature for 240 minutes to prepare modified polyacrylic acid;

[0119] Take 10 parts of polyvinyl alcohol and add deionized water, heat to 92°C and stir to dissolve, cool to 69°C, add activated carbon powder, 10 parts of dicyclohexylcarbodiimide, and 0.3 parts of 4-dimethylaminopyridine in sequence and mix evenly, keep the temperature constant for 18 hours, maintain the system temperature, add modified polyacrylic acid and stir to mix, take the mixed spherical product and freeze-dry to form a carbon layer wrapped by the modified resin to prepare the scaffold layer.

[0120] (2) Preparation of microbial agents:

[0121] 16.5 parts of acrylic acid monomer was dissolved in a solvent, and a photoinitiator was added to prepare a solution;

[0122] Take the microbial agent and add it into deionized water, stir and disperse it to prepare a suspension;

[0123] Take 1.5 parts of agar and add it to deionized water, heat it to 77°C and stir to dissolve, cool it to 40°C, add the suspension and stir it thoroughly, add it dropwise to the solution at a temperature of 27°C, let it stand for 25 minutes to form a microbial agent matrix wrapped with the microbial agent, and prepare the microbial agent.

[0124] (3) Preparation of treatment agent:

[0125] The scaffold layer was mixed evenly with the bacterial agent, placed under light conditions, and slowly stirred at room temperature for 240 minutes to prepare a treatment agent.

[0126] Comparative Example 5

[0127] (1) Preparation of scaffold layer:

[0128] The carbon source was crushed and dehydrated, spread on a concave-convex plate with honeycomb protrusions, pressed and dried, and then crushed to obtain a flaky carbon layer with a diameter of 3 to 10 mm. The flaky carbon layer was mixed with an aqueous solution of starch, stirred at 87°C for 2 hours, and carbonized at high temperature in an argon atmosphere to obtain a spherical carbon frame. The carbonization process was as follows: under a pressure of -0.05 MPa, the heating rate was increased from room temperature to 850°C at a rate of 10°C / min, and the spherical carbon frame was obtained after constant temperature treatment for 1.5 hours.

[0129] The spherical carbon frame is treated with a mixed acid solution of concentrated sulfuric acid and concentrated nitric acid, and then placed at a constant temperature of 90°C for 180 hours. The product is dried to form a carbon layer with a flaky skeleton.

[0130] Take 0.7 parts of agar and add deionized water, heat to 77°C and stir to dissolve, add 15 parts of acrylic acid monomer and 0.7 parts of photoinitiator, cool in the dark, and place under light to react at room temperature for 240 minutes to prepare modified polyacrylic acid;

[0131] Take 10 parts of polyvinyl alcohol and add deionized water, heat to 92°C and stir to dissolve, cool to 69°C, add carbon layer, 10 parts of dicyclohexylcarbodiimide, and 0.3 parts of 4-dimethylaminopyridine in sequence and mix evenly, keep the temperature constant for 18 hours, maintain the system temperature, add modified polyacrylic acid and stir to mix, take the mixed spherical product and freeze-dry to form a carbon layer wrapped by modified resin to prepare the scaffold layer.

[0132] (2) Preparation of microbial agents:

[0133] Take the microbial agent and add it into deionized water, stir and disperse it to prepare a suspension;

[0134] Take 1.5 parts of agar and add it to deionized water, heat it to 77°C and stir to dissolve, cool it to 40°C, add the suspension and stir it thoroughly, add it dropwise into deionized water at a temperature of 27°C, let it stand for 25 minutes to form a microbial agent matrix wrapped with the microbial agent, and prepare the microbial agent.

[0135] (3) Preparation of treatment agent:

[0136] The scaffold layer was mixed evenly with the bacterial agent, placed under light conditions, and slowly stirred at room temperature for 240 minutes to prepare a treatment agent.

[0137] Comparative Example 6

[0138] (1) Preparation of scaffold layer:

[0139] The carbon source is crushed and dehydrated, spread on a concave-convex plate with honeycomb protrusions, pressed and dried, and then crushed to obtain a flaky carbon layer with a diameter of 3 to 10 mm. The flaky carbon layer is mixed with an aqueous solution of starch, stirred at 87°C for 2 hours, and carbonized at high temperature in an argon atmosphere to obtain a spherical carbon frame. The carbonization process is: under a pressure of -0.05 MPa, the heating rate is 10°C / min from room temperature to 850°C. After constant temperature treatment for 1.5 hours, the product is obtained as the support layer.

[0140] (2) Preparation of microbial agents:

[0141] The microbial agent is added into deionized water, stirred and dispersed to prepare a suspension as the microbial agent.

[0142] (3) Preparation of treatment agent:

[0143] The scaffold layer was mixed evenly with the bacterial agent, and slowly stirred at room temperature for 240 minutes to prepare the treatment agent.

[0144] experiment

[0145] The treating agents obtained in Examples 1-5 and Comparative Examples 1-6 were used to prepare samples, and their properties were tested and the test results were recorded:

[0146] Among them, the performance test includes the activity and adsorption performance test of the immobilized microorganisms;

[0147] The experimental steps for the activity of immobilized microorganisms are as follows: a sample is placed in 37°C pure water and artificially waved. After treatment for 12 hours, 14 days, and 6 minutes, 100 mL of inorganic salt culture medium containing 150 mg / L m-cresol is added. The culture medium is aerated to saturate the dissolved oxygen, then aeration is stopped. The temperature is maintained at 30°C and the culture medium is waved. The change in dissolved oxygen in the solution over time is monitored using a dissolved oxygen meter, and the amount of oxygen consumed per unit time is used as the technical indicator.

[0148] The adsorption performance test procedure is as follows: Place the sample in 37°C pure water and artificially create waves. After treatment for 12 hours, 14 days, and 6 minutes, add it in batches to 80 mL of m-cresol solution and cadmium chloride with an initial concentration of approximately 150 mg / L. Maintain the temperature at 30°C and create waves in the culture medium. Samples are taken regularly to analyze the residual concentration of m-cresol, and the adsorption capacity of the carrier (mg / g) is calculated. The adsorption capacity of the sample after 1 hour is used as the experimental indicator.

[0149]

[0150] According to the data in the above table, we can clearly draw the following conclusions:

[0151] The treatment agents obtained in Examples 1-5 were compared with the treatment agents obtained in Comparative Examples 1-6. The test results show that:

[0152] 1. Compared with the treatment agents obtained in Comparative Examples 1-6, the treatment agents obtained in Examples 1-5 showed improved oxygen consumption and adsorption capacity experimental data after 12 h, 14 d, and 6 m. This fully demonstrates that the leachate treatment agent prepared by the present invention has good microbial activity and adsorption performance at different stages and can continuously exert its effectiveness.

[0153] 2. The treatment agent obtained in Example 2 is compared with the treatment agents obtained in Comparative Examples 1-6:

[0154] Compared with Example 2, the preparation of the carbon layer in Comparative Example 1 is different, the components dicyclohexylcarbodiimide and 4-dimethylaminopyridine are not added in Comparative Example 2, the surface modification resin of the carbon layer in Comparative Example 3 is different, the activated carbon and the modified resin are blended to prepare the scaffold layer in Comparative Example 4, and no acrylic acid monomer is added to the bacterial agent matrix in Comparative Example 5. Comparative Example 6 is a mixture of a carbon layer and a microbial agent. The experimental data of oxygen consumption and adsorption capacity of the treatment agents prepared in Comparative Examples 1-6 after 12h, 14d, and 6m are different. From the data and the comparison of different stages, it can be seen that the setting of the components and preparation process in the leachate treatment agent in the present invention has a promoting effect on its good microbial activity and adsorption performance at different stages, and is conducive to the sustainability of the treatment agent's effectiveness.

[0155] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0156] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or substitute equivalents for some of the technical features. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A method for preparing a microbial treatment agent for landfill leachate, characterized in that: The following steps are involved: (1) Preparation of the scaffold layer: The carbon source is crushed and dehydrated, spread on a concave-convex plate with honeycomb protrusions, pressed and dried, and then crushed to obtain a sheet-like carbon layer with a diameter of 3 to 10 mm; The flaky carbon layer is mixed with a starch aqueous solution, stirred at 80-95°C for 1-3 hours, and carbonized at high temperature in an argon atmosphere to obtain a spherical carbon frame; The spherical carbon frame is treated with a mixed acid solution of concentrated sulfuric acid and concentrated nitric acid, and then placed at a constant temperature of 88 to 92°C for 150 to 200 minutes, and the product is dried to form a carbon layer with a flaky skeleton; Add deionized water to agar, heat to 75-80°C, stir and dissolve, add acrylic acid monomer and photoinitiator, cool in the dark, and react under light at room temperature for 200-280 minutes to prepare modified polyacrylic acid; Add polyvinyl alcohol to deionized water, heat to 90-94°C, stir and dissolve, cool to 68-70°C, add a carbon layer, dicyclohexylcarbodiimide, and 4-dimethylaminopyridine in sequence, and mix evenly. Maintain the temperature for 12-24 hours, then add modified polyacrylic acid and stir to mix. The spherical product after the mixture is freeze-dried to form a carbon layer wrapped by the modified resin, thereby preparing the scaffold layer. (2) Preparation of bacterial agent: dissolve acrylic acid monomer in solvent, add photoinitiator to prepare solution; Take the microbial agent and add it into deionized water, stir and disperse it to prepare a suspension; Add agar to deionized water, heat to 75-80°C, stir and dissolve, cool to 38-42°C, add the suspension, stir thoroughly, add dropwise to the solution at 20-35°C, let stand for 20-30 minutes, and prepare the inoculum. (3) Preparation of treatment agent: Mix the scaffold layer with the bacterial agent and irradiate with light to obtain the treatment agent; The carbon source is inedible plants and their roots, stems and leaves discarded from farmers' markets and / or planting bases.

2. The method for preparing a microbial treatment agent for landfill leachate according to claim 1, characterized in that: The step (3) includes the following steps: The scaffold layer and the bacterial agent were mixed evenly, placed under light conditions, and slowly stirred at room temperature for 200 to 280 minutes to prepare a treatment agent.

3. The method for preparing a microbial treatment agent for landfill leachate according to claim 1, characterized in that: The density of the carbon layer in step (1) is 0.5 to 0.8 g / cm³.

4. The method for preparing a microbial treatment agent for landfill leachate according to claim 1, characterized in that: The carbonization process of step (1) is as follows: under a pressure of -0.1 to 0 MPa, the temperature is raised from room temperature to 800 to 900°C at a heating rate of 10°C / min, and the temperature is kept constant for 1 to 2 hours.

5. The method for preparing a microbial treatment agent for landfill leachate according to claim 1, characterized in that: The modified resin is prepared from the following components by weight: 0.5-1 parts of agar, 10-21 parts of acrylic acid monomer, 0.2-1.3 parts of photoinitiator, 8-12 parts of polyvinyl alcohol, 3-18 parts of dicyclohexylcarbodiimide, and 0.1-0.5 parts of 4-dimethylaminopyridine.

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