A method and kit for evaluating the differentiation state of stem cells

By designing primers near the target gene region for multiple PCR amplification and high-throughput sequencing, the problems of less gene coverage and complex detection processes in the prior art are solved, and the effect of quickly and accurately assessing the differentiation status of stem cells is achieved.

CN116103371BActive Publication Date: 2025-06-13HANGZHOU REPUGENE TECH CO LTD
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Patent Information

Application Number
CN202310150617.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Priority Date
2022-09-07
Filing Date
2023-02-22
Publication Date
2025-06-13
Estimated Expiration
2043-02-22

AI Technical Summary

Technical Problem

In the prior art, when detecting stem cell differentiation status, there is a problem of less gene coverage or complex detection process, long-term and high cost.

Method used

Multiple PCR amplification by designing primers near the target gene region and high-throughput sequencing of the PCR products can be quickly and efficiently obtained the expression of the target gene.

Benefits of technology

The rapid and accurate evaluation of stem cell differentiation status is achieved, which improves the accuracy and speed of detection and reduces costs.

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Abstract

The present invention belongs to the technical field of gene detection, and particularly relates to a method and a kit for evaluating the differentiation state of stem cells. The method first screens out genes related to the differentiation of stem cells to be evaluated as target genes, designs primers for the screened target genes, performs multiplex PCR amplification, and performs high-throughput sequencing on the PCR products, so as to quickly and efficiently obtain the expression of the target genes, thereby helping to quickly and accurately evaluate the differentiation state of stem cells.
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