Use of cryptosporidium cgd6_2310 protein as an oocyst outer wall marker protein

By using Cryptosporidium cgd6_2310 protein as a marker protein for the outer wall of the oocyst, a specific antibody was prepared, which solved the problem of low sensitivity in existing Cryptosporidium detection methods and achieved efficient and economical oocyst wall detection, suitable for monitoring environmental and clinical samples.

CN116144677BActive Publication Date: 2026-02-03JILIN UNIVERSITY
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Patent Information

Application Number
CN202210804248.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-07-09
Publication Date
2026-02-03
Estimated Expiration
2042-07-09

AI Technical Summary

Technical Problem

The lack of specific antibodies in existing technologies for labeling the outer wall of Cryptosporidium oocysts results in low sensitivity and high cost in Cryptosporidium detection methods, making them difficult to apply on a large scale, especially when the oocyst content in environmental water samples is diluted.

Method used

Cryptosporidium cgd6_2310 protein was used as a marker protein for the outer wall of the oocyst. Specific antibodies were prepared and detected by indirect immunofluorescence. The application of recombinant protein and peptide antibodies was combined to achieve specific recognition and enrichment of the oocyst wall.

Benefits of technology

It improves the sensitivity and specificity of Cryptosporidium detection, reduces detection costs, and is suitable for efficient monitoring of environmental water samples and clinical samples, with broad application potential.

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Abstract

The application discloses application of a cryptosporidium cgd6_2310 protein as an oocyst outer wall marker protein, an amino acid sequence of which is shown in the sequence table SEQ ID N0.1; a cryptosporidium oocyst wall protein specific polypeptide, an amino sequence of which is CLTPQSIERKEGTIID or ELDSSRTPVNETINC; an anti-cryptosporidium oocyst outer wall protein antibody, which can specifically bind to the outer surface of the cryptosporidium oocyst outer wall; an anti-cryptosporidium oocyst outer wall specific polypeptide antibody, which is an antibody prepared by using the cryptosporidium oocyst wall protein specific polypeptide; application of the anti-cryptosporidium oocyst outer wall protein antibody or the anti-cryptosporidium oocyst outer wall protein specific polypeptide antibody in detection of cryptosporidium; the polypeptide rabbit serum antibody has good sensitivity; in detection of samples in the environment, the polypeptide antibody only recognizes the cryptosporidium oocyst and does not cross react, and has good specificity.
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