Application of 2-cyclohexenol in antibacterial, promoting plant growth, and / or inducing plant resistance

Abstract

The present invention provides an application of 2-bornanol in antibacterial, promoting plant growth, and / or inducing plant resistance, and also provides a method for antibacterial, promoting plant growth, and / or inducing plant resistance, as well as a biological pesticide, a plant resistance inducer, and a plant growth regulator. The present invention directly clarifies the feasibility of 2-bornanol as a plant growth regulator, a resistance inducer, and a biological pesticide and is proven in field trials. Compared with other methods of biological pesticides and growth regulators, the application and method of the present invention have the advantages of simple operation, low requirements for outdoor spraying conditions, and can be popularized and applied in the field.

Description

Technical Field

[0001] The present invention relates to the field of agriculture, and particularly to the application of 2-cyclohexenol in antibacterial, promoting plant growth, and / or inducing plant resistance; the present invention also relates to a method for antibacterial, promoting plant growth, and / or inducing plant resistance, and to a biological pesticide, a plant resistance inducer, and a plant growth regulator. Background Art

[0002] Ginseng (Panax ginseng C.A.MEY) is a perennial shade-loving herbaceous plant of the genus Panax in the Araliaceae family. Its active ingredient, ginsenoside, has an impact on the immune system, central nervous system, cardiovascular and cerebrovascular systems, and anti-tumor effects. Ginseng can be classified into three categories according to the cultivation mode: forest-clearing ginseng cultivation, farmland ginseng cultivation, and under-forest ginseng cultivation. "Forest-clearing ginseng cultivation", as the traditional cultivation mode of ginseng in China, has caused serious damage to forest resources and the ecological environment. This cultivation mode is gradually being transformed into farmland ginseng cultivation and under-forest ginseng cultivation, effectively solving the contradiction between ginseng and forest land. However, black spot disease, as one of the most common and harmful diseases of ginseng above the ground, causes economic losses that increase year by year along with the 5-6-year growth cycle of ginseng. Therefore, a large amount of pesticides, fertilizers, and bacterial fertilizers need to be added during the planting process to control diseases and improve the seedling retention rate and yield of ginseng, resulting in serious over-standard of agricultural residues and heavy metals in the produced medicinal materials, and even the emergence of drug resistance in ginseng pathogens. With the increasing awareness of environmental protection and drug safety in recent years, pollution-free production and pest control of ginseng have become urgent problems that need to be solved in the current ginseng industry.

[0003] Currently, the main technologies for pollution-free disease control of ginseng in farmland cultivation are mainly three types: agricultural control technology, chemical control technology, and biological control technology. Agricultural control creates an environment conducive to the growth of medicinal materials but unfavorable for the occurrence of pests and diseases by improving cultivation management measures, achieving the purpose of controlling the occurrence and spread of pests and diseases, including a series of agronomic measures such as land selection, seed selection, soil preparation, fertilization, water management, shading, and intertillage and weeding. Due to the immaturity of the cultivation technology system, it is limited by the costs of human and material resources in actual production. Although chemical control avoids the use of pesticides prohibited or restricted for traditional Chinese medicines, with the increasing sophistication of trace agricultural residue detection technology, the agricultural residues caused by chemical control directly limit the export trade of ginseng. Biological control can be classified according to its source into: 1) animal-derived pesticides; 2) plant-derived pesticides; 3) microorganism-derived pesticides. Among them, plant-derived pesticides and microorganism-derived pesticides are the focus of research, development, and application. Plant secondary metabolites such as asarum volatile oil, alcohol extracts and water extracts of traditional Chinese medicines such as cloves, patchouli, and magnolia officinalis, and microbial strains or their metabolites such as Bacillus subtilis, Trichoderma harzianum, and Bacillus amyloliquefaciens all have a high inhibition rate against ginseng black spot disease.

[0004] Biological control, which uses beneficial organisms and their metabolites to prevent diseases and control pathogens, has become the focus of the development of pollution-free agricultural production due to its advantages such as environmental friendliness, high biological activity, safety for humans, and low resistance generation. However, there is still relatively little research on plant-derived pesticides for ginseng diseases. Most biogenic antibacterial tests only stay at the laboratory stage, and only some have been promoted and applied to the fields, and there are still problems such as unstable antibacterial activity and insignificant control effect per plant.

[0005] Plant-derived pesticides are a type of bio-pesticide with a high degree of harmony with the environment, which are developed by screening and formulating natural substances. Currently, systematically studied plant-derived pesticides include nicotine, celangulin, rotenone, sabadilla, etc. Their characteristics are good control effects on pests and diseases, easy decomposition and no residue in the environment, controlling pests and diseases by directly inhibiting pathogens or inducing plants to produce resistance, and low resistance generation. As a popular research field in the development of bio-pesticides in the world in recent years, plant elicitors can enter plants in various ways and be recognized by plant receptors to stimulate plants to produce hypersensitive reactions, activate a series of defense response systems such as plant defense-related hormone pathways, accumulate phytoalexins, and protein inhibitors that inhibit pathogen degradation enzymes to obtain resistance (SAR, systemic acquired resistance); or change plant root exudates to regulate rhizosphere microorganisms in the soil and induce plants to produce resistance (ISR, induced systemic resistance) by recruiting beneficial bacteria.

[0006] Plant regulators are widely used in seed germination and plant growth and development. To varying degrees, they have significant regulation on the seed germination, sprouting, rooting, emergence, and agronomic traits, flowering and fruiting, and dry and fresh weight accumulation of different plants, ultimately increasing yield and improving quality. In recent years, it has been found that plant extracts can be applied to plant regulation (such as the 778 inducer), including multiple effects such as growth control, cold resistance, drought resistance, waterlogging tolerance, pest and disease control, and high yield. It can be foreseen that plant-derived growth regulators can be used as green pesticide synergists and directly applied to seed germination, plant growth, and field crops. CN114503842A discloses a method for controlling blueberry anthracnose. This method uses a combination of biogenic pesticides and plant elicitors for spraying, which significantly controls the occurrence of blueberry anthracnose. The involved biogenic pesticides and plant elicitors are harmless to humans and livestock and do not pollute the environment, ensuring the high-quality production of blueberry fruits. CN114532363A discloses a method for using and application of a seed coating containing Trichoderma. It is a seed bio-coating agent formula with Trichoderma harzianum spores as the main functional component, which has the functions of promoting plant growth and controlling soil-borne diseases. After application, it has a significant growth-promoting effect on the growth and development of plants and can significantly reduce the incidence of plant soil-borne diseases.

[0007] However, current biogenic pesticides are mostly microbial pesticides. Spraying requires suitable temperature and humidity, and the efficacy can only be fully exerted within a specific environmental range. Some biopesticides also have poor photo-stability and are prone to losing activity under sunlight irradiation. Therefore, their residual efficacy period is short after field application and the efficacy cannot be exerted. In addition, some biopesticides on the market have a single efficacy and a narrow range of pests and diseases that can be controlled; most plant growth regulators on the market have the characteristics of high use cost, low broad-spectrum property, and poor use safety performance. These deficiencies are difficult to keep pace with the development speed of agriculture and forestry.

[0008] The present invention discovers that borneol is a multi-functional botanical pesticide with the characteristics of a bacteriostatic agent, a plant growth regulator, and an elicitor. It can both inhibit the pathogens of ginseng in the field and promote plant growth. Summary of the Invention

[0009] In view of the problems existing in the prior art, the present invention provides an application of borneol in bacteriostasis, promoting plant growth, and / or inducing plant resistance, as well as related methods and agents.

[0010] Borneol, commonly known as borneol, is mostly present in plants of Lauraceae, Pinaceae, and Compositae. It has been widely used in edible flavors, the pharmaceutical industry, essence, and the cosmetic raw material industry, and has the characteristics of low cost and high safety. The present invention discovers that borneol can promote plant growth, induce plant resistance, and inhibit the growth of ginseng pathogenic bacteria. It is a potential biopesticide, plant elicitor, and plant growth regulator. The induced resistance has broad-spectrum property, can effectively reduce field diseases, is easy to degrade without residue, avoids environmental pollution, and has low cost.

[0011] Therefore, in one aspect, the present invention provides an application of borneol in bacteriostasis, promoting plant growth, and / or inducing plant resistance.

[0012] In a preferred embodiment, the bacteriostasis includes inhibiting plant pathogens, such as ginseng fungal pathogens; preferably, the bacteriostasis includes inhibiting Botrytis cinerea and Alternaria panax.

[0013] In a preferred embodiment, the plants include ginseng, corn, lettuce, broad beans, etc.

[0014] In a preferred embodiment, the induction of plant resistance includes inducing disease resistance in plants, and the disease resistance includes resistance to bacteria and fungi, such as resistance to fungal pathogens of ginseng. Preferably, the disease resistance includes resistance to Botrytis cinerea and Alternaria panax.

[0015] In a preferred embodiment, the induction of plant resistance is achieved by regulating the rhizosphere microbial community and recruiting beneficial bacteria. The beneficial bacteria include fungi such as Trichoderma, Gymnoascus, Fusidium, Tolypocladium, Knufia, Ambispora, etc.; bacteria such as Nocardioides, Adhaeribacter, Burkholderia, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Catenulispora, Streptomyces, Ramlibacter, Pseudomonas, Nitrospira, Haliangium, etc.

[0016] In a preferred embodiment, the application includes treating plants with 2-borneol, including treating the seeds of plants with 2-borneol, drenching the roots of plant plants, or spraying the leaves.

[0017] In a preferred embodiment, 2-borneol can be formulated with excipients into an agriculturally acceptable dosage form during application, and the dosage form includes sprays, emulsifiable concentrates, aqueous solutions, suspensions, etc.

[0018] Preferably, 2-bornanol is used in the form of a solution, especially an aqueous solution. The concentration of the 2-bornanol solution can be 0.001 - 1000 mg / L, preferably 0.001 mg / L, 0.005 mg / L, 0.01 mg / L, 0.05 mg / L, 0.1 mg / L, 0.5 mg / L, 1 mg / L, 1 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, 30 mg / L, 40 mg / L, 50 mg / L, 60 mg / L, 70 mg / L, 80 mg / L, 90 mg / L, 100 mg / L, 200 mg / L, 300 mg / L, 500 mg / L, 1000 mg / L. Preferably, the concentration of the 2-bornanol solution can be 0.01 - 100 mg / L. When used for antibacterial purposes, the concentration of the 2-bornanol solution can be 10 - 300 mg / L, preferably 10 - 100 mg / L; when used for promoting plant growth, the concentration of the 2-bornanol solution can be 0.01 - 100 mg / L, preferably 0.01 - 10 mg / L; when used for inducing plant resistance, the concentration of the 2-bornanol solution can be 0.01 - 10 mg / L, preferably 0.01 - 1 mg / L.

[0019] In a second aspect, the present invention provides a method for antibacterial, promoting plant growth, and / or inducing plant resistance, the method comprising treating with 2-bornanol.

[0020] In a preferred embodiment, the antibacterial includes inhibiting plant pathogens, such as ginseng fungal pathogens; preferably, the antibacterial includes inhibiting Botrytis cinerea and Alternaria panax.

[0021] In a preferred embodiment, the plants include ginseng, corn, lettuce, broad beans, etc.

[0022] In a preferred embodiment, the inducing plant resistance includes inducing plant disease resistance, and the disease resistance includes resistance to bacteria and fungi, such as resistance to ginseng fungal pathogens. Preferably, the disease resistance includes resistance to Botrytis cinerea and Alternaria panax.

[0023] In a preferred embodiment, inducing plant resistance includes regulating the rhizosphere microbial community and recruiting beneficial bacteria. The beneficial bacteria include fungi such as Trichoderma, Gymnoascus, Fusidium, Tolypocladium, Knufia, Ambispora, etc.; bacteria such as Nocardioides, Adhaeribacter, Burkholderia, Allorhizobium - Neorhizobium - Pararhizobium - Rhizobium, Catenulispora, Streptomyces, Ramlibacter, Pseudomonas, Nitrospira, Haliangium, etc.

[0024] In a preferred embodiment, the method includes treating the plant with 2 - borneol, including treating the seeds of the plant with 2 - borneol, drenching the plant or spraying on the leaf surface.

[0025] In a preferred embodiment, 2 - borneol can be formulated with excipients into an agriculturally acceptable dosage form when applied, and the dosage form includes sprays, emulsion in water, aqueous solutions, suspensions, etc.

[0026] Preferably, 2 - borneol is used in the form of a solution, especially an aqueous solution. The concentration of the 2 - borneol solution can be 0.001 - 1000 mg / L, preferably 0.001 mg / L, 0.005 mg / L, 0.01 mg / L, 0.05 mg / L, 0.1 mg / L, 0.5 mg / L, 1 mg / L, 1 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, 30 mg / L, 40 mg / L, 50 mg / L, 60 mg / L, 70 mg / L, 80 mg / L, 90 mg / L, 100 mg / L, 200 mg / L, 300 mg / L, 500 mg / L, 1000 mg / L. Preferably, the concentration of the 2 - borneol solution can be 0.01 - 100 mg / L. When used for antibacterial, the concentration of the 2 - borneol solution can be 10 - 300 mg / L, preferably 10 - 100 mg / L; when used for promoting plant growth, the concentration of the 2 - borneol solution can be 0.01 - 100 mg / L, preferably 0.01 - 10 mg / L; when used for inducing plant resistance, the concentration of the 2 - borneol solution can be 0.01 - 10 mg / L, preferably 0.01 - 1 mg / L.

[0027] In a third aspect, the present invention provides a biological pesticide, a plant resistance inducer, and a plant growth regulator.

[0028] In the present invention, the "Biopesticide" refers to a preparation that uses living organisms, or substances with biological activity produced during the biological metabolism process, or substances extracted from organisms to control diseases, pests, weeds, and rodents of agricultural and forestry crops, and can support the commercial circulation of products, including fungicides, biological insecticides, agricultural antibiotics, ecological pesticides, etc.

[0029] In a preferred embodiment, the biopesticide includes a fungicide, which is used to inhibit plant pathogens, such as ginseng fungal pathogens; preferably, the bacteriostasis includes inhibiting Botrytis cinerea and Alternaria panax.

[0030] In the present invention, the "plant growth regulator" refers to a class of substances that regulate various metabolic processes in plants to improve crop quality and increase yield.

[0031] In a preferred embodiment, the plant growth regulator is used to promote plant growth.

[0032] In the present invention, the "promoting plant growth" includes promoting plant seed germination, biomass accumulation, root growth, improving plant quality, and increasing yield.

[0033] In the present invention, the "plant elicitor" refers to a general term for factors that can activate plants to produce defense responses.

[0034] In a preferred embodiment, the plant elicitor is used to induce plants to produce resistance, including inducing plants to produce disease resistance, and the disease resistance includes resistance to bacteria and fungi, such as resistance to ginseng fungal pathogens. Preferably, the disease resistance includes resistance to Botrytis cinerea and Alternaria panax.

[0035] In a preferred embodiment, the "inducing plants to produce resistance" includes achieving it by regulating the rhizosphere microbial community and recruiting beneficial bacteria. The beneficial bacteria include fungi such as Trichoderma, Gymnoascus, Fusidium, Tolypocladium, Knufia, Ambispora, etc.; bacteria such as Nocardioides, Adhaeribacter, Burkholderia, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Catenulispora, Streptomyces, Ramlibacter, Pseudomonas, Nitrospira, Haliangium, etc.

[0036] In a preferred embodiment, the plants include ginseng, corn, lettuce, broad beans, etc.

[0037] In a preferred embodiment, the biological bacteriostatic agent, plant resistance inducer, and plant growth regulator may further include other excipients and may be in the form of sprays, emulsion in water, aqueous solutions, suspensions, etc.

[0038] Preferably, 2-borneol is formulated into a solution, especially in the form of an aqueous solution for use. The concentration of the 2-borneol solution can be 0.001 - 1000 mg / L, preferably 0.001 mg / L, 0.005 mg / L, 0.01 mg / L, 0.05 mg / L, 0.1 mg / L, 0.5 mg / L, 1 mg / L, 1 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, 30 mg / L, 40 mg / L, 50 mg / L, 60 mg / L, 70 mg / L, 80 mg / L, 90 mg / L, 100 mg / L, 200 mg / L, 300 mg / L, 500 mg / L, 1000 mg / L. Preferably, the concentration of the 2-borneol solution can be 0.01 - 100 mg / L. When used for bacteriostasis, the concentration of the 2-borneol solution can be 10 - 300 mg / L, preferably 10 - 100 mg / L; when used for promoting plant growth, the concentration of the 2-borneol solution can be 0.01 - 100 mg / L, preferably 0.01 - 10 mg / L; when used for inducing plant resistance, the concentration of the 2-borneol solution can be 0.01 - 10 mg / L, preferably 0.01 - 1 mg / L.

[0039] Beneficial effects:

[0040] Aiming at the disadvantage that the efficacy of existing biological pesticides is easily restricted and interfered by environmental factors, the present invention uses the bacteriostatic effect and resistance induction effect of the compound 2-borneol in pine needles and its growth-promoting effect on various crops. By spraying the leaves of ginseng in the field, the growth of pathogenic bacteria in ginseng in the field is reduced and the growth of plants is promoted, ultimately effectively controlling the occurrence of ginseng field diseases and facilitating the accumulation of ginseng root biomass. The resistance induced by 2-borneol is broad-spectrum.

[0041] The present invention directly clarifies the feasibility of 2-borneol as a plant growth regulator, resistance inducer, and biological pesticide and has been proven in field trials. Utilizing the bacteriostatic effect of 2-borneol itself, the growth of pathogenic bacteria of Alternaria leaf spot and Botrytis cinerea can be effectively inhibited. At the same time, it also has the effect of promoting plant growth. Ultimately, whether under low or high concentration treatments, 2-borneol can effectively reduce diseases and benefit the accumulation of crop yields. The low concentration mainly reduces the occurrence of diseases by regulating rhizosphere microorganisms to induce plant resistance; the high concentration alleviates diseases by directly inhibiting bacteria and recruiting beneficial rhizosphere bacteria to activate the induced resistance of plants.

[0042] Compared with other methods of biological pesticides and growth regulators, the application and method of the present invention have the advantages of simple operation, low requirements for outdoor spraying conditions (such as temperature and humidity), and can be popularized and applied in the fields. The induced resistance is broad-spectrum, which can effectively reduce field diseases. Moreover, because it is easily degraded and has no residue, it can avoid environmental pollution and has low cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 : Activity of 2-borneol in inhibiting ginseng fungal pathogens;

[0044] Figure 2 : Situation of 2-borneol promoting plant growth;

[0045] Figure 3 : Field trial of 2-borneol in inhibiting Alternaria panax;

[0046] Figure 4 : Situation of 2-borneol regulating rhizosphere microorganisms;

[0047] Figure 5 : 2-borneol promoting the accumulation of ginseng biomass. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0048] The present invention is described in more detail below to facilitate the understanding of the present invention.

[0049] It should be understood that the terms or words used in the specification and claims should not be construed as having the meanings defined in the dictionary, but should be understood as having meanings consistent with their meanings in the context of the present invention based on the following principles: The concept of the terms can be appropriately defined by the inventor for the best description of the present invention.

[0050] Example 1: Bacteriostatic Effect of Borneol

[0051] 2-Camphene can significantly inhibit the growth of the mycelia of the pathogens of ginseng black spot and gray mold. The mother liquor was prepared by dissolving 2-camphene in 60% ethanol to form a series of diluted solutions, which were aseptically filtered through an organic filter membrane with a pore size of 0.22 μm and a diameter of 25 mm and reserved. 1 ml of the mother liquor of the corresponding concentration was added to every 99 ml of PDA (Potato Dextrose Agar) medium, and 1 ml of 60% ethanol that had been aseptically filtered was added to the control group. After shaking well, it was poured into a petri dish to make a plate, and a series of PDA media with 2-camphene concentrations of 0, 10, 25, 50, 100, 200, 400, 800, and 1000 mg / L were obtained. After the plate solidified, a test bacterial cake with a diameter of 5 mm and consistent growth was inoculated in the center of each dish (this example involves the antibacterial tests of two pathogens: Botrytis cinerea, the pathogen of gray mold, and Alternaria panax, the pathogen of black spot). Each treatment was repeated 6 times. After culturing at 25°C for 7 days, the results were observed, and the cross method was used to measure the diameter of the colony growth. Inhibition rate (%) = (control colony diameter - treated colony diameter) × 100 / (control colony diameter - 0.5 cm). The results are as Figure 1 shown.

[0052] 2-Camphene can significantly inhibit the accumulation of the mycelial biomass of the pathogens of black spot and gray mold. 1 ml of the mother liquor of the corresponding concentration was added to every 99 ml of PDB (Potato Dextrose Broth) medium, and 1 ml of 60% ethanol that had been aseptically filtered was added to the control group, and a series of PDB media with 2-camphene concentrations of 0, 10, 25, 50, 100, 200, 400, 800, and 1000 mg / L were obtained. After shaking well, 3 test bacterial cakes with a diameter of 5 mm and consistent growth were added to each bottle of the medium, and each concentration treatment was repeated 5 times. It was cultured in a shaker at 25°C and 110 r / min for 4 days, and the fermentation broth was removed by filtering through four layers of gauze. The fermentation broth on the mycelia was slowly rinsed clean with water and dried in an oven at 60°C for 3 days until a constant weight was reached, and the dry weight of the mycelia was weighed. Calculate the inhibition rate of 2-camphene on the mycelial biomass. Inhibition rate (%) = (control mycelial dry weight - treated mycelial dry weight) × 100 / (control colony dry weight - bacterial cake dry weight). The results are as Figure 1 shown.

[0053] As Figure 1 , 2-camphene has strong activity in inhibiting ginseng fungal pathogens and can limit the growth of ginseng pathogens as the concentration increases. The results of the antibacterial test show that the EC 50 (half lethal concentration) of 2-camphene against the pathogen of ginseng black spot is 175.71 mg / L, and the EC 50 against the pathogen of ginseng gray mold is 141.44 mg / L. The pathogen of gray mold is more sensitive to 2-camphene than the pathogen of black spot.

[0054] Example 2: Growth-promoting effects of 2-camphol on different crops

[0055] The tested seeds included plants reported in the literature to be sensitive to allelopathic effects: lettuce, broad bean, and corn. A series of aqueous solutions of 2-camphol with concentration gradients of 0, 0.01, 0.1, 1, 10, 50, and 100 mg / L were set up for the experiment. Five replicates were set for each concentration gradient. Broad beans and peanuts were cultivated in soil. According to the suitable temperatures provided in the literature (25 °C for lettuce, 20 °C for broad beans, 20 °C for corn), the seeds were planted in sterilized soil and placed in a light incubator for cultivation (12 hours of light and 12 hours of darkness), and the seeds were buried at the same depth in the soil. Lettuce seeds were cultured using the filter paper method. The seeds were evenly placed in a glass petri dish lined with three layers of filter paper, and different concentrations of aqueous 2-camphol solutions were added. Equal amounts of solution were replenished regularly for each treatment. With the emergence of the radicle as the germination marker, the number of germinated seeds was recorded regularly every day until the recorded number was the same as that of the previous day. Seed germination rate = (number of normally germinated seeds / total number of tested seeds) × 100%. At a specific number of days after sowing the seeds (7 days for lettuce, 16 days for broad beans, 30 days for peanuts), the plant height of the seedlings was measured. Then, the soil on the plant roots was carefully washed, dried in an oven at 75 °C to a constant weight, and then weighed for its dry weight. The results are as Figure 2 shown.

[0056] As Figure 2 shown, different crops have different sensitivities to 2-camphol, and the effective concentrations for promoting growth are also different. The germination rates of corn and broad bean seeds were not significantly affected by the regulation of 2-camphol concentration, but the accumulation of biomass and plant height were significantly promoted when the 2-camphol concentration was 1 - 10 mg / L and 0.01 mg / L, respectively. When the 2-camphol concentration was 0.1 mg / L, the germination rate, biomass accumulation, and root length of lettuce seeds were significantly promoted.

[0057] Example 3: Field experiment on 2-camphol for controlling Alternaria panax Whetz

[0058] A series of aqueous solutions of 2-camphol with concentration gradients of 0, 0.01, 0.1, 1, 10, 50, and 100 mg / L were prepared. Two-year-old and four-year-old Panax ginseng at the leaf-expanding stage in Laobiangu, Benxi Manchu Autonomous County, Liaoning Province were used as the research objects. Six plots were set for each concentration treatment, and each plot was 4 m 2 . From June 10th to August 24th, each concentration treatment group was sprayed once a week, with limited dripping. Through the natural pathogen-induced disease experiment, waiting for the natural occurrence of diseases in the field, the incidence of Alternaria panax Whetz on ginseng in the field was counted on August 30th, and its disease index was calculated. The results are as Figure 3 shown.

[0059] As Figure 3, with the increase in the concentration of 2-cholesterol, the incidence and disease index of black spot disease in biennial and four-year-old ginseng significantly decreased. According to the degree of reduction of black spot disease, the application concentration of 2-cholesterol can be divided into low concentration and high concentration ranges, which are 0.01 - 1 mg / L and 10 - 100 mg / L respectively. In the low concentration range, the strongest effect of reducing black spot disease is 0.1 mg / L. The incidence and disease index of black spot disease in biennial ginseng are 10.80% and 6.35% respectively, and those in four-year-old ginseng are 38% and 20% respectively. In the high concentration range, the best 2-cholesterol concentrations for disease inhibition are the highest 2 tested concentrations, which are 50 and 100 mg / L respectively, corresponding to the lowest incidence and disease index of black spot disease in biennial (0.35%, 0.12%) and four-year-old (23.8%, 8.9%).

[0060] Example 4: Field experiment on the induction of plant resistance by 2-cholesterol through recruiting beneficial rhizosphere microorganisms

[0061] Carefully dig out the healthy plants in each treatment group in Example 3, and carefully collect the ginseng root soil with a soft brush into a 50 ml centrifuge tube. After quenching with liquid nitrogen, send it to the laboratory to measure the microbial diversity of each treatment group. The results are as Figure 4 shown.

[0062] The results of Example 1 ( Figure 1 ) show that at a concentration of 10 mg / L of 2-cholesterol, the effect of inhibiting the black spot pathogen is not significant. However, in the results of Example 3 ( Figure 3 ), 0.1 mg / L in the low concentration range shows a significant disease inhibition effect. Considering the literature, it is considered that at this concentration of 2-cholesterol, plant root exudates regulate rhizosphere microorganisms, thereby activating the induced resistance of plants to resist the invasion of black spot pathogens.

[0063] Figure 4 The results show that foliar spraying of 2-cholesterol at different concentrations will change the relative abundances of the genus level of the ginseng rhizosphere microbial community. Low concentration (0 - 1 mg / L) and high concentration (10 - 100 mg / L) 2-cholesterol regulate rhizosphere fungi and bacteria to varying degrees. The relative abundances of 132 fungal genera are significantly regulated by the concentration of 2-cholesterol: 53 fungal genera are regulated by the low concentration range, and 79 fungal genera are regulated by the high concentration range. Among them, the fungal genera beneficial to plant growth significantly up-regulated by low concentration 2-cholesterol include Trichoderma, Gymnoascus, etc.; the fungal genera significantly up-regulated by high concentration include Fusidium, Tolypocladium, Knufia, etc.; the fungal genera significantly up-regulated by both low and high concentrations such as Ambispora, etc. Some of these fungi can directly inhibit fungi / bacteria / nematodes, and some secrete volatiles and secondary metabolites to facilitate plant resistance regulation and growth promotion, thereby improving plant resistance.

[0064] The relative abundances of 145 rhizobacterial genera were significantly regulated by 2-borneol, among which the relative abundances of 70 genera were significantly down-regulated and those of 75 genera were significantly up-regulated. Among the up-regulated genera, there are rhizosphere microorganisms widely reported to promote plant growth. Among them, Nocardioides, Adhaeribacter, etc. were up-regulated by low-concentration 2-borneol; Burkholderia, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Catenulispora were up-regulated by high-concentration 2-borneol; the genera that were significantly up-regulated under both low and high concentrations of 2-borneol are: Streptomyces, Ramlibacter, Pseudomonas, Nitrospira, Haliangium. These plant growth-promoting bacteria can either secrete auxin to promote plant growth, or assist plants in nitrogen fixation and phosphorus solubilization to improve the absorption of root nutrients by plants, or induce plants to produce resistance to control pests and diseases.

[0065] Combined with the test results of Example 4, it can be seen that spraying low and high concentrations of 2-borneol can regulate different microbial communities to recruit beneficial bacteria, and ultimately can effectively reduce the occurrence of black spot disease. Example 5: Field test on 2-borneol promoting the biomass accumulation of ginseng

[0066] After collecting the rhizosphere soil in Example 4, carefully wash the roots of ginseng with gentle water flow, dry them in an oven at 50 °C to constant weight, and weigh the total dry weight of the underground part, the dry weight of the rhizome, the dry weight of the main root, and the dry weight of the fibrous roots. The results are as Figure 5 shown.

[0067] Generally, plants with lower incidence and disease index have better growth and biomass accumulation. The test results also show that: in the low-concentration range, when the 2-borneol concentration is 0.1 mg / L, the biomass of the underground part, main root, fibrous roots and rhizome increases significantly ( Figure 5 ). In the high-concentration range, in the 50 mg / L and 100 mg / L treatment groups with lower incidence and disease index, the biomass accumulation is also the best ( Figure 5 ).

[0068] The test results show that 2-borneol has the characteristics of antibacterial, promoting plant growth and inducing plant resistance. When it is applied to the prevention and control of Alternaria panax Whetz in the field: spraying low-concentration (0-1 mg / L) 2-borneol on the leaf surface can regulate the recruitment of beneficial microorganisms in the rhizosphere, thereby promoting the plant to produce resistance to reduce the occurrence of Alternaria panax Whetz, and at the same time being beneficial to plant growth; spraying high-concentration (10-100 mg / L) 2-borneol can not only inhibit bacteria by 2-borneol itself, but also regulate the rhizosphere microbial community to recruit beneficial bacteria to assist in resisting the invasion of Alternaria panax Whetz pathogens, thereby reducing the occurrence of Alternaria panax Whetz disease and helping plant growth.

[0069] The preferred embodiments of the present invention have been described above, but they are not intended to limit the present invention. Those skilled in the art can make improvements and changes to the embodiments disclosed herein without departing from the scope and spirit of the present invention.

Claims

1. Use of 2-bornanol in antibacterial, where the antibacterial is to inhibit Alternaria panax, a pathogen of black spot disease among plant pathogens.

2. The application according to claim 1, wherein The plant is Panax ginseng.

3. The application according to claim 1, wherein The use includes treating the plant with 2-bornanol.

4. The application according to claim 3, characterized in that Treat the seeds of the plant with 2-bornanol, or drench the roots or spray the leaves of the plant.

5. The application according to claim 1, characterized in that When applied, 2-bornanol is made into an agriculturally acceptable formulation with excipients, and the formulation includes spray, emulsion in water, aqueous solution, and suspension.

6. The application according to claim 5, wherein 2-Bornanol is used in the form of an aqueous solution.

7. A method for antibacterial with 2-bornanol, the method includes treating with 2-bornanol, and the antibacterial is to inhibit Alternaria panax, a pathogen of black spot disease among plant pathogens.

8. The method according to claim 7, wherein The plant is Panax ginseng.

9. The method according to claim 7, characterized in that, The method includes treating the plant with 2-bornanol.

10. The method according to claim 9, wherein Treat the seeds of the plant with 2-bornanol, or drench the roots or spray the leaves of the plant.

11. The method according to claim 7, wherein When applied, 2-bornanol is made into an agriculturally acceptable formulation with excipients, and the formulation includes spray, emulsion in water, aqueous solution, and suspension.

12. The method according to claim 11, wherein 2-Bornanol is used in the form of an aqueous solution.

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