An ovarian follicle near-infrared fluorescent probe and a preparation method and application thereof

The near-infrared fluorescent probe for follicles, prepared by conjugating indocyanine green-succinimide ester with human chorionic gonadotropin, solves the problems of ineffective detection of early follicles and high probe cost in existing technologies, and achieves highly specific targeted imaging and rapid metabolism of follicle imaging effects.

CN116924963BActive Publication Date: 2026-07-07FUDAN UNIVERSITY
View PDF 1 Cites 0 Cited by

Patent Information

Application Number
CN202210371148.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-04-11
Publication Date
2026-07-07
Estimated Expiration
2042-04-11

AI Technical Summary

Technical Problem

Existing follicle imaging technology cannot effectively detect early follicles, and existing near-infrared fluorescent probes suffer from high cost, scarcity of raw materials, and low clinical conversion rate.

Method used

A near-infrared fluorescent probe for follicles was prepared by conjugating indocyanine green-succinimide with human chorionic gonadotropin (hCG). Taking advantage of the abundance of hCG and the high fluorescence quantum yield of indocyanine green, and combining specific targeting of ovarian cells, the preparation method includes dissolution, mixing, conjugation, and purification steps.

Benefits of technology

It achieves highly specific targeted imaging of follicles, with deep penetration depth, high spatial resolution, no toxic side effects, and rapid biological metabolism, reducing costs and improving clinical translational value.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN116924963B_ABST
    Figure CN116924963B_ABST
Patent Text Reader

Abstract

The present application adopts human chorionic gonadotropin and indocyanine green-succinimidyl ester to carry out coupling reaction, and provides an ovarian follicle near-infrared fluorescent probe, and the preparation method of the probe is as follows: indocyanine green-succinimidyl ester solution and human chorionic gonadotropin solution are added into phosphate buffer solution with pH of 7.4-7.5 according to a proper ratio to obtain a mixed solution, stirring is carried out to react, and then the product is purified. The ovarian follicle near-infrared fluorescent probe provided by the present application has the advantages of rich raw material source, low cost, high clinical transformation and application value compared with the prior art, and has the characteristics of strong specificity, good light stability, deep penetration, high spatial resolution, fast biological metabolic rate, stable observation time window, no toxic side effects, and the like. The preparation method of the probe is simple in operation, good in stability and repeatability. The present application further provides an ovarian follicle contrast agent containing the ovarian follicle near-infrared fluorescent probe, and the contrast agent has high targeting, strong contrast, no toxic side effects and fast biological metabolic rate.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • Follicular granulocyte near-infrared fluorescent probe Nirova-1 and preparation method and application thereof

    CN111234813A