Antigen for enhancing immune effect of newcastle disease virus and preparation method and application thereof

By constructing the Ii-key-F-Fc recombinant vector fusion protein, the problem of insufficient immunity in Newcastle disease virus vaccines was solved, and the local and systemic immune responses in poultry were enhanced through the mucosal immune pathway, thereby improving the control effect of Newcastle disease virus.

CN116970091BActive Publication Date: 2026-08-04ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202310873865.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-07-17
Publication Date
2026-08-04
Estimated Expiration
2043-07-17

AI Technical Summary

Technical Problem

Existing Newcastle disease virus vaccines have shortcomings such as susceptibility to maternal antibody interference, incomplete protection, and virulence reversion. Furthermore, there is a lack of effective vaccines that can be administered via the mucosal immune route, making it difficult to induce local mucosal immunity and systemic immune responses in poultry.

Method used

A fusion protein was constructed using the Ii-key-F-Fc recombinant vector. The three major antigenic epitope fragments of the NDV F protein were tandemly linked with chicken IgY Fc and Ii-key, and the Ii-key-F-Fc fusion protein was prepared using an E. coli expression system for mucosal immunization to enhance the immune effect of Newcastle disease virus.

Benefits of technology

It achieved the goal of stimulating the body to produce effective local and systemic immune responses through the mucosal immune pathway, thereby improving the immunity of poultry to Newcastle disease virus and reducing the incidence of disease.

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Abstract

This invention relates to the field of animal immunology, and more particularly to an antigen that enhances the immune response to Newcastle disease virus (NDV), its preparation method, and its application. The antigen is a fusion protein obtained by expressing the Ii-key-F-Fc recombinant vector using engineered bacteria, named the Ii-key-F-Fc fusion protein. The Ii-key-F-Fc recombinant vector is obtained by splicing three major antigenic epitope fragments from the NDV F protein and then tandemly connecting them with chicken IgY Fc and Ii-key. This invention constructs the pCold-TF-Ii-key-F-Fc prokaryotic expression plasmid by tandemly connecting the novel chicken vector Ii-key-Fc with the F antigenic epitope, and then expresses the fusion protein using an E. coli expression system to obtain a new antigen that enhances the immune response in poultry. This provides a new approach for developing a novel NDV vaccine and a new strategy for improving the immune response of subunit vaccines in poultry.
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