An anti-aging composition and a method of preparing the same

Abstract

The present application relates to an anti-aging composition and a preparation method thereof, and belongs to the technical field of cosmetics.The anti-aging composition comprises, in mass parts, 0.3-0.8 parts of nicotinamide adenine dinucleotide, 0.1-0.4 parts of acetyl tetrapeptide-11, 0.3-0.8 parts of p-hydroxyacetophenone, 0.3-0.8 parts of 1,2-hexanediol, 0.1-0.4 parts of 1,2 pentanediol, 0.5-1 parts of acetylated sodium hyaluronate, 8-12 parts of butanediol and 100 parts of water.The anti-aging composition can be used to prepare anti-aging cosmetics suitable for various skin types, and has strong cosmetic efficacy and good stability through the compounding of nicotinamide adenine dinucleotide, acetyl tetrapeptide-11 and p-hydroxyacetophenone, and can not only reduce existing facial wrinkles, but also effectively prevent the generation of new wrinkles.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to an anti-aging composition and a preparation method thereof. BACKGROUND

[0002] Skin is the largest organ of the human body, which is wrapped on the surface of the body and directly contacts with the external environment, and has various physiological functions such as barrier function, feeling external stimulation, regulating body temperature or excretion. Since the skin is often in contact with various external stimuli, compared with other organs, the skin is more prone to problems. In addition, with the increase of age, work and life pressure, combined with environmental and food factors, the loss and degradation of collagen, elastin and other components in the skin are becoming more and more serious, leading to skin aging and wrinkle, which has caused serious distress to people, especially to women who love beauty. Therefore, how to effectively delay skin aging or alleviate the symptoms of aging has been a hot spot of research and attention worldwide.

[0003] At present, most of the representative anti-aging cosmetics use functional raw materials, such as sodium hyaluronate, retinoids, vitamin C, vitamin E, lycopene, ursolic acid, bovine serum albumin, etc. However, these materials have strong irritation to the skin, the stability of the substances themselves is poor, and the anti-aging effect is limited. Therefore, the use of multiple components for compounding is the main research direction at present. Invention patent CN 109568376 A discloses an anti-aging traditional Chinese medicine composition, which is an anti-aging composition composed of Poria cocos, acanthopanax, gynostemma pentaphyllum and malva sylvestris. However, its anti-aging effect is limited and requires a specific preparation method to obtain. Invention patent CN 107837204 A discloses a narrow distribution particle size emulsion with skin anti-aging effect and a preparation method thereof, the emulsion contains 2.5%-10% of anti-aging composition, 0.5%-10% of emulsifier, 5-20% of oil, 65-85% of water; the anti-aging composition is composed of ribose, camphane glycol, pinane glycol, dimethyl sulfone and nicotinamide adenine dinucleotide in a mass ratio of 1:0.5-2:0.5-2:1-3:0.5-3. However, its anti-skin aging effect is general. Invention patent CN 108771643 A discloses a rich and moist repair essence, which contains a composition of 0.1-10 parts by weight of acetylated chitosan, 0.1-10 parts by weight of Stephania tetrandra extract, 0.1-10 parts by weight of Malus hallings seed oil, 0.1-10 parts by weight of lavender oil and 0.1-10 parts by weight of nicotinamide adenine dinucleotide, which has a certain cure effect, but is mainly aimed at sensitive skin.

[0004] In summary, although there are various anti-aging products on the market at present, the effect is not obvious enough. Therefore, it is still a technical problem to be solved urgently to develop an anti-aging composition to meet the needs of various skin types. SUMMARY

[0005] To solve the above technical problems, the present application provides an anti-aging composition and a preparation method thereof.

[0006] The first object of the present application is to provide an anti-aging composition comprising nicotinamide adenine dinucleotide 0.3-0.8 parts by mass, acetyl tetrapeptide-1 0.1-0.4 parts by mass, p-hydroxyacetophenone 0.3-0.8 parts by mass, 1,2-hexanediol 0.3-0.8 parts by mass, 1,2 pentanediol 0.1-0.4 parts by mass, acetylated sodium hyaluronate 0.5-1 parts by mass, butanediol 8-12 parts by mass, and water 100 parts by mass.

[0007] In one embodiment of the present application, it comprises nicotinamide adenine dinucleotide 0.5 parts by mass, acetyl tetrapeptide-1 0.2 parts by mass, p-hydroxyacetophenone 0.5 parts by mass, 1,2-hexanediol 0.5 parts by mass, 1,2 pentanediol 0.2 parts by mass, acetylated sodium hyaluronate 0.8 parts by mass, butanediol 10 parts by mass, and water 100 parts by mass.

[0008] The second object of the present application is to provide a preparation method of the anti-aging composition, comprising the following steps: stirring and mixing the components of the anti-aging composition according to the mass ratio to obtain the anti-aging composition.

[0009] The third object of the present application is to provide an anti-aging cosmetic prepared from the anti-aging composition.

[0010] In one embodiment of the present application, the anti-aging composition is added in an amount of 20%-30% of the mass of the anti-aging cosmetic.

[0011] In one embodiment of the present application, the anti-aging cosmetic further comprises the following auxiliary materials: thickening agent, emulsifying agent, preservative, and complexing agent.

[0012] In one embodiment of the present application, the thickening agent is selected from one or more of hydroxyethyl cellulose, carboxypropyl cellulose, methyl cellulose, ethyl cellulose, and xanthan gum.

[0013] In one embodiment of the present application, the emulsifying agent is selected from one or more of cetyl stearyl glucoside, cetyl alcohol, glyceryl stearate, cetyl glucoside, stearyl alcohol, lanolin, and cocoglycoside.

[0014] In one embodiment of the present application, the preservative is selected from one or more of potassium sorbate, phenoxyethanol, p-hydroxyacetophenone, and hydroxypropyl benzoate.

[0015] In one embodiment of the present application, the complexing agent is selected from disodium ethylenediaminetetraacetate and / or tetrasodium ethylenediaminetetraacetate.

[0016] In an embodiment of the present application, the anti-aging cosmetic is a water agent, a cream, a paste, an oil agent, an emulsion, a mask or a serum.

[0017] The technical solution of the present application has the following advantages compared with the prior art:

[0018] (1) The p-hydroxyacetophenone used in the anti-aging composition has strong antioxidant and anti-glycosylation ability, can scavenge active oxygen free radicals ROS and alpha-beta unsaturated aldehyde generated in the oxidative stress process, protect and promote collagen production, protect the skin from damage by free radicals and oxidative stress. It can inhibit the activity of tyrosinase, delay skin aging, and whiten the skin. In addition, p-hydroxyacetophenone has sunscreen ability, which can prevent and reduce the damage of this wavelength of ultraviolet light to the skin. P-hydroxyacetophenone can stimulate nicotinamide adenine dinucleotide, increase cell respiration, promote the synthesis of extracellular matrix components, activate skin vitality to reduce wrinkles, improve skin elasticity and brighten skin color; p-hydroxyacetophenone is well matched with nicotinamide adenine dinucleotide and acetyl tetrapeptide-11, further improving the anti-aging effect of the composition.

[0019] (2) The nicotinamide adenine dinucleotide used in the anti-aging composition is a coenzyme in cells, which can promote cell metabolism and make cells more active; it can interfere with the cell signal channel between keratinocytes and melanocytes, reduce melanin production, reduce the transfer of melanin to the surface cells, stimulate collagen production, increase the level of free fatty acids and ceramides in the skin, reduce skin water loss, control oil and shrink pores, and has the effects of removing wrinkles, increasing skin elasticity, shrinking pores, anti-aging, improving skin barrier, moisturizing and making the skin smoother, and has good popularization prospects.

[0020] (3) The acetyl tetrapeptide-11 used in the anti-aging composition is an active peptide, which can double-lock melanocyte from the inside and outside, fade stubborn pigmentation, tightly lock tyrosinase, and prevent the penetration of tyrosine, so as to achieve the purpose of inhibiting the formation of melanin. By inhibiting the release of neurotransmitter acetylcholine, it can reduce muscle contraction, thereby reducing the formation of dynamic wrinkles and expression wrinkles, and has excellent effects on removing wrinkles, increasing skin elasticity, shrinking pores and lightening pigmentation.

[0021] (4) The anti-aging composition can be used to prepare anti-aging cosmetics suitable for various skin types. Through the compounding of nicotinamide adenine dinucleotide, acetyl tetrapeptide-11, p-hydroxyacetophenone and the like, the cosmetic has strong efficacy and good stability, which can not only reduce existing facial wrinkles, but also effectively prevent the formation of new wrinkles. DETAILED DESCRIPTION

[0022] The present application will be further described in greater detail by way of specific embodiments, so as to provide a better understanding of the present application and to enable the present application to be practiced. It is to be understood that the specific embodiments described are merely illustrative of one part of the present application and are not intended to limit the present application.

[0023] In the present application, unless otherwise stated, the technical and scientific terms used in the present application have the same meanings as those commonly understood by the person skilled in the art to which the present application belongs.

[0024] In the present application, unless otherwise stated, the term "and / or" used in the present application includes any and all combinations of one or more of the relevant listed items.

[0025] In the present application, unless otherwise stated, the experimental methods used in the embodiments of the present application are conventional methods, and the materials, reagents, etc. used are commercially available, unless otherwise stated.

[0026] In the present application, unless otherwise stated, the "parts" used in the embodiments of the present application refer to mass parts.

[0027] Example 1

[0028] The anti-aging composition and the preparation method thereof of the present application specifically include the following steps:

[0029] 1,2-hexanediol 0.5 parts, 1,2 pentanediol 0.2 parts, sodium acetylated hyaluronic acid 0.8 parts, butanediol 10 parts and water 100 parts are added into a stirring pot, stirred and dissolved, nicotinamide adenine dinucleotide 0.5 parts, acetyl tetrapeptide-1 0.2 parts, p-hydroxyacetophenone 0.5 parts are continuously added, and stirring is uniform to obtain the anti-aging composition.

[0030] Example 2

[0031] The anti-aging composition and the preparation method thereof of the present application specifically include the following steps:

[0032] 1,2-hexanediol 0.8 parts, 1,2 pentanediol 0.1 parts, sodium acetylated hyaluronic acid 1 part, butanediol 8 parts and water 100 parts are added into a stirring pot, stirred and dissolved, nicotinamide adenine dinucleotide 0.8 parts, acetyl tetrapeptide-1 0.1 parts, p-hydroxyacetophenone 0.8 parts are continuously added, and stirring is uniform to obtain the anti-aging composition.

[0033] Example 3

[0034] The anti-aging composition and the preparation method thereof of the present application specifically include the following steps:

[0035] 1,2-hexanediol 0.3 parts, 1,2 pentanediol 0.4 parts, sodium acetylated hyaluronic acid 0.5 parts, butanediol 12 parts and water 100 parts are added into a stirring pot, stirred and dissolved, nicotinamide adenine dinucleotide 0.3 parts, acetyl tetrapeptide-1 0.4 parts, p-hydroxyacetophenone 0.3 parts are continuously added, stirred and uniformly mixed to obtain the anti-aging composition.

[0036] Example 4

[0037] The anti-aging composition and the preparation method thereof of the present application specifically include the following steps:

[0038] 1,2-hexanediol 0.8 parts, 1,2 pentanediol 0.4 parts, sodium acetylated hyaluronic acid 1 part, butanediol 12 parts and water 100 parts are added into a stirring pot, stirred and dissolved, nicotinamide adenine dinucleotide 0.8 parts, acetyl tetrapeptide-1 0.4 parts, p-hydroxyacetophenone 0.8 parts are continuously added, stirred and uniformly mixed to obtain the anti-aging composition.

[0039] Example 5

[0040] The anti-aging composition and the preparation method thereof of the present application specifically include the following steps:

[0041] 1,2-hexanediol 0.3 parts, 1,2 pentanediol 0.1 parts, sodium acetylated hyaluronic acid 0.5 parts, butanediol 8 parts and water 100 parts are added into a stirring pot, stirred and dissolved, nicotinamide adenine dinucleotide 0.3 parts, acetyl tetrapeptide-1 0.1 parts, p-hydroxyacetophenone 0.3 parts are continuously added, stirred and uniformly mixed to obtain the anti-aging composition.

[0042] Comparative Example 1

[0043] 1,2-hexanediol 0.5 parts, 1,2 pentanediol 0.2 parts, sodium acetylated hyaluronic acid 0.8 parts, butanediol 10 parts and water 100 parts are added into a stirring pot, stirred and dissolved, acetyl tetrapeptide-1 0.2 parts, p-hydroxyacetophenone 0.5 parts are continuously added, stirred and uniformly mixed to obtain the anti-aging composition.

[0044] Comparative Example 2

[0045] 1,2-hexanediol 0.5 parts, 1,2 pentanediol 0.2 parts, sodium acetylated hyaluronic acid 0.8 parts, butanediol 10 parts and water 100 parts are added into a stirring pot, stirred and dissolved, nicotinamide adenine dinucleotide 0.5 parts, p-hydroxyacetophenone 0.5 parts are continuously added, stirred and uniformly mixed to obtain the anti-aging composition.

[0046] Comparative Example 3

[0047] Into a stirring pot were put 1,2-hexanediol 0.5 parts, 1,2 pentanediol 0.2 parts, sodium acetylated hyaluronic acid 0.8 parts, butanediol 10 parts and water 100 parts, and stirred and dissolved. Then, nicotinamide adenine dinucleotide 0.5 parts was further added, and stirred and mixed to obtain an anti-aging composition.

[0048] Comparative Example 4

[0049] Into a stirring pot were put 1,2-hexanediol 0.5 parts, 1,2 pentanediol 0.2 parts, sodium acetylated hyaluronic acid 0.8 parts, butanediol 10 parts and water 100 parts, and stirred and dissolved. Then, nicotinamide adenine dinucleotide 0.5 parts was further added, and stirred and mixed to obtain an anti-aging composition.

[0050] Comparative Example 5

[0051] Into a stirring pot were put 1,2-hexanediol 0.5 parts, 1,2 pentanediol 0.2 parts, sodium acetylated hyaluronic acid 0.8 parts, butanediol 10 parts and water 100 parts, and stirred and dissolved. Then, acetyl tetrapeptide-1 0.2 parts was further added, and stirred and mixed to obtain an anti-aging composition.

[0052] Comparative Example 6

[0053] Into a stirring pot were put 1,2-hexanediol 0.5 parts, 1,2 pentanediol 0.2 parts, sodium acetylated hyaluronic acid 0.8 parts, butanediol 10 parts and water 100 parts, and stirred and dissolved. Then, p-hydroxyacetophenone 0.5 parts was further added, and stirred and mixed to obtain an anti-aging composition.

[0054] Test Example 1

[0055] (1) Heat resistance test: First, an electric heating incubator was adjusted to 40±1℃, and one sample each of the compositions of Examples 1-5 and Comparative Examples 1-6 were placed in the electric heating incubator for 24 hours. After that, the samples were taken out and allowed to recover to room temperature. Then, the samples of Examples 1-5 and Comparative Examples 1-6 were compared with the original samples, and whether there were phenomena such as thinning, color change, layering and hardness change were observed to determine the heat resistance of the products.

[0056] (2) Cold resistance test: First, a refrigerator was adjusted to -10℃±1℃, and one sample each of the compositions of Examples 1-5 and Comparative Examples 1-6 were placed in the refrigerator for 24 hours. After that, the samples were taken out and allowed to recover to room temperature. Then, the samples of Examples 1-5 and Comparative Examples 1-6 were compared with the original samples, and whether there were phenomena such as thinning, color change, layering and hardness change were observed to determine the cold resistance of the products.

[0057] The specific results of the heat resistance and cold resistance are shown in Table 1.

[0058] Table 1

[0059]

[0060]

[0061] As shown in Table 1, the compositions prepared in Examples 1-5 all have excellent stability, whereas the compositions prepared in Comparative Examples 1-6 all have poor stability and are difficult to use as cosmetic materials.

[0062] Test Example 2

[0063] (1) Measurement of DPPH radical scavenging ability: 3 mL of a phosphate buffer having a pH of 6.8, 4 mL of a 0.4 mmol / L DPPH ethanol solution, and 0.2 mL of the composition of Examples 1-5 and Comparative Examples 1-6 were sequentially added to a 10 mL centrifuge tube, mixed well, and allowed to stand for 30 min. After centrifugation for 10 min (8000 r / min), the supernatant was taken, and the absorbance value was measured at 516 nm. The average value was obtained from three parallel operations, and the DPPH radical scavenging rate of the sample was calculated as follows: [1 - (A1 - A2) / A3]; wherein A1 is the absorbance value of the DPPH solution after reaction with the sample, A2 is the absorbance value of the sample solution without DPPH (ethanol was used instead), and A3 is the absorbance value of the DPPH solution without the sample.

[0064] (2) Measurement of superoxide anion radical scavenging ability: A buffer system (Tris-HCl-EDTA) having a pH of 8.20 was prepared. In a 10 mL centrifuge tube, 0.6 mL of the composition of Examples 1-5 and Comparative Examples 1-6 was added, 5.2 mL of the buffer was added, 0.2 mL of a 0.045 mol / L pyrogallol solution was added using a pipette, and the mixture was shaken well. After 5 min, the absorbance value was measured in a dry cuvette at 325 nm, and was recorded as A0. After 10 min, the absorbance value was measured again and was recorded as A4. The absorbance values measured using distilled water instead of the sample were recorded as B0 and B4, respectively. The average value was obtained from three parallel operations, and the superoxide anion radical scavenging rate of the sample was calculated as follows: [(B4 - B0) - (A4 - A0)] / (B4 - B0).

[0065] (3) The determination of the ability to scavenge hydroxyl radicals: 1 mL (0.4 mmol / L) of crystal violet, 3 mL (0.2 mol / L) of pH 7.4 phosphate buffer, 1 mL (0.001 mol / L) of FeSO4 solution, 1 mL of the composition of Example 1-5 and Comparative Example 1-6 were added into a 10 mL centrifuge tube, and 1 mL of 30% H2O2 was added to initiate the reaction. After reaction at room temperature for 1 h, centrifugation was performed at 12000 r / min for 5 min, and the supernatant was taken to determine the absorbance at 510 nm, which was recorded as C. The absorbance when distilled water was used instead of the sample was recorded as C1, and the absorbance when distilled water was used instead of the sample and H2O2 was recorded as C2. The average value was obtained by parallel operation for 3 times, and the scavenging rate of the sample on hydroxyl radicals was (C-C1) / (C2-C1);

[0066] The ability to scavenge DPPH radicals (%), the ability to scavenge superoxide anion radicals (%) and the ability to scavenge hydroxyl radicals (%) are shown in Table 2:

[0067] Table 2

[0068] Test sample DPPH radical scavenging ability Superoxide anion radical scavenging ability Hydroxyl radical scavenging ability Example 1 96.63 95.46 99.65 Example 2 96.54 95.37 99.58 Example 3 96.68 95.50 99.69 Example 4 96.79 95.60 99.84 Example 5 96.34 95.11 99.23 Comparative Example 1 84.54 83.65 88.76 Comparative Example 2 83.49 82.47 87.63 Comparative Example 3 84.67 84.12 89.51 Comparative Example 4 56.49 55.61 59.43 Comparative Example 5 56.93 55.87 59.84 Comparative Example 6 55.36 54.49 59.29

[0069] As can be seen from Table 2, the composition of Example 1-5 has a significantly better ability to scavenge DPPH radicals, superoxide anion radicals and hydroxyl radicals than the composition of Comparative Example 1-6. When any one of nicotinamide adenine dinucleotide, acetyl tetrapeptide-11 and p-hydroxyacetophenone is absent in Comparative Example 1-3, the scavenging effect is obviously decreased; when any two of nicotinamide adenine dinucleotide, acetyl tetrapeptide-11 and p-hydroxyacetophenone are absent in Comparative Example 4-6, the scavenging effect is worse, which indicates that the synergistic effect among nicotinamide adenine dinucleotide, acetyl tetrapeptide-11 and p-hydroxyacetophenone in the anti-aging composition of the present application is significant, and the effect of significantly lightening skin wrinkles is achieved, and the purpose of anti-aging is achieved.

[0070] Test Example 3

[0071] Six groups of mice were taken, and each group had 3 mice. Five groups were rubbed with the compositions prepared in Example 1-5 and Comparative Example 1-6, respectively, and the other group was not applied with any product (blank group). The back skin of the mice was irradiated by ultraviolet rays, and the degree of redness, thickening, desquamation, shrinkage and the like was observed, and the results are shown in Table 3:

[0072] Table 3

[0073] Test sample Clearness of back wrinkles Redness level Desquamation Blank group Severe 3 Severe Example 1 None 0 Mild Example 2 None 0 Mild Example 3 None 0 Mild Example 4 None 0 Mild Example 5 None 0 Mild Comparative Example 1 General 1 General Comparative Example 2 General 1 General Comparative Example 3 General 1 General Comparative Example 4 Severe 3 Severe Comparative Example 5 Severe 3 Severe Comparative Example 6 Severe 3 Severe

[0074] As can be seen from Table 3, the compositions of Examples 1-5 have good protective effect on the back epidermis of mice. When any one of nicotinamide adenine dinucleotide, acetyl tetrapeptide-11 and p-hydroxyphenylacetone is absent in Comparative Examples 1-3, the protective effect is obviously decreased; when any two of nicotinamide adenine dinucleotide, acetyl tetrapeptide-11 and p-hydroxyphenylacetone are absent in Comparative Examples 4-6, the protective effect is poor, which indicates that the synergistic effect among nicotinamide adenine dinucleotide, acetyl tetrapeptide-11 and p-hydroxyphenylacetone in the anti-aging composition of the present application is significant, which can significantly fade skin wrinkles and achieve the purpose of anti-aging.

[0075] Obviously, the above examples are only examples for clearly illustrating, and are not limitation to the embodiments. Other different forms of changes or variations can be made by those skilled in the art on the basis of the above description. All the embodiments do not need to be exhausted here. The obvious changes or variations derived therefrom are still within the protection scope of the present application.

Claims

1. An anti-aging composition, characterized in that, 0.3-0.8 parts by mass of nicotinamide adenine dinucleotide, 0.1-0.4 parts by mass of acetyl tetrapeptide-1, 0.3-0.8 parts by mass of p-hydroxyacetophenone, 0.3-0.8 parts by mass of 1,2-hexanediol, 0.1-0.4 parts by mass of 1,2 pentanediol, 0.5-1 parts by mass of acetylated sodium hyaluronate, 8-12 parts by mass of butylene glycol, and 100 parts by mass of water.

2. A method of preparing the anti-aging composition of claim 1, characterized by, The anti-aging composition is prepared by mixing the components in the proportions by mass.

3. An anti-aging cosmetic product, characterized by: The anti-aging cosmetic is prepared from the anti-aging composition of claim 1.

4. The anti-aging cosmetic product according to claim 3, characterized in that, The anti-aging composition is added in an amount of 20%-30% by mass of the anti-aging cosmetic.

5. The anti-aging cosmetic product according to claim 3, characterized in that, The anti-aging cosmetic further comprises the following auxiliary materials: a thickening agent, an emulsifying agent, a preservative, and a complexing agent.

6. The anti-aging cosmetic product according to claim 5, characterized in that, The thickening agent is selected from one or more of hydroxyethyl cellulose, carboxypropyl cellulose, methyl cellulose, ethyl cellulose, and xanthan gum.

7. The anti-aging cosmetic product according to claim 5, wherein The emulsifying agent is selected from one or more of cetyl stearyl glucoside, cetyl alcohol, glyceryl stearate, cetyl glucoside, stearyl alcohol, lanolin, and coco glucoside.

8. The anti-aging cosmetic product according to claim 5, wherein The preservative is selected from one or more of potassium sorbate, phenoxyethanol, p-hydroxyacetophenone, and hydroxypropyl benzoate.

9. The anti-aging cosmetic product according to claim 5, wherein The complexing agent is selected from disodium ethylenediaminetetraacetate and / or tetrasodium ethylenediaminetetraacetate.

10. The anti-aging cosmetic product according to claim 3, characterized in that, The anti-aging cosmetic is an aqueous agent, a cream, a paste, an oil, an emulsion, a mask, or a serum.

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