Automatic analyzer and specimen analysis method

Abstract

In the past, in the light scattering detection method, a high concentration sample deviating from the quantitative range required retesting, requiring a long measurement time. Therefore, for the reaction solution of the sample and the reagent, the first light amount value is obtained from the absorbance photometer, and the second light amount value is obtained from the scattering photometer. According to the second light amount value at the first measurement point determined in advance, it is determined whether quantitative analysis based on the second light amount value can be performed. In the case where it is determined that quantitative analysis based on the second light amount value is not performed, the reagent is additionally dispensed into the reaction solution, and quantitative analysis based on the first light amount value at the measurement point after the additional dispensing is performed.

Claims

1. An automatic analysis device, characterized in that, have: A reaction disk, which has units arranged on its circumference to contain the reaction solution containing the sample and reagents; The sample dispensing mechanism dispenses the sample into the unit on the reaction plate; A reagent dispensing mechanism that dispenses reagents into the units on the reaction plate; A spectrophotometer that measures light transmitted from a first light source and from a reaction liquid contained in a unit on the reaction plate; A scattering photometer measures light scattered by the reaction liquid contained in the unit on the reaction disk after being illuminated by the second light source. A control circuit that drives the reaction disk, the sample dispensing mechanism, and the reagent dispensing mechanism; as well as The data processing unit executes the specimen measurement procedure and controls the control circuit according to the specimen measurement procedure. For the reaction solution of the sample dispensed by the sample dispensing mechanism and the reagent dispensed by the reagent dispensing mechanism, the data processing unit obtains a first light intensity value from the absorber and a second light intensity value from the scattering spectrophotometer. Based on the second light intensity value at a predetermined first photometric point, it determines whether quantitative analysis based on the second light intensity value can be performed. If it is determined that quantitative analysis based on the second light intensity value cannot be performed, the reagent is additionally dispensed into the reaction solution through the reagent dispensing mechanism, and quantitative analysis is performed based on the first light intensity value at the photometric point after the additional dispensing.

2. The automatic analysis device according to claim 1, characterized in that, When the data processing unit determines that it is possible to perform quantitative analysis based on the second light intensity value, it performs quantitative analysis based on the second light intensity value at the photometer points before and after the first photometer point.

3. The automatic analysis device according to claim 2, characterized in that, The data processing unit maintains a first calibration curve for quantitative analysis based on the first light intensity value and a second calibration curve for quantitative analysis based on the second light intensity value. The first calibration curve is generated based on the first photometric value obtained for standard solutions of different concentrations, under measurement conditions where quantitative analysis based on the second photometric value in the sample measurement procedure is deemed impossible. The second calibration curve is a calibration curve generated based on the second photometric value obtained for standard solutions of different concentrations, according to the measurement conditions determined to be suitable for quantitative analysis based on the second photometric value in the sample measurement procedure.

4. The automatic analysis device according to claim 1, characterized in that, If the second light intensity value at the first photometric point exceeds a preset threshold light intensity, the data processing unit determines that quantitative analysis based on the second light intensity value cannot be performed.

5. The automatic analysis device according to claim 4, characterized in that, The data processing unit sets the threshold light intensity based on the second light intensity value obtained according to the measurement conditions when quantitative analysis based on the second light intensity value is determined to be possible in the specimen measurement procedure for the standard solution.

6. The automatic analysis device according to claim 1, characterized in that, In the spectrophotometer, the first light source and the transmitted light receiver are arranged with respect to units on the reaction disk. In the scattering photometer, the second light source and the scattered light receiver are arranged with respect to units on the reaction disk. The time interval between the photometers measuring the light transmitted from the reaction liquid by the absorber, or the time interval between the photometers measuring the light scattered by the reaction liquid by the scatterer, is the time it takes for the reaction disk to rotate once.

7. The automatic analysis device according to claim 1, characterized in that, The automatic analysis device includes a display unit that displays a parameter setting screen for setting the measurement conditions in the sample measurement procedure. The parameter setting screen includes a selection section for choosing whether to add the reagent to the reaction solution.

8. A method for analyzing a sample using an automated analysis device, the automated analysis device comprising: a reaction disk having a unit arranged circumferentially thereon containing a sample and a reagent reaction liquid; a sample dispensing mechanism dispensing a sample into the unit on the reaction disk; a reagent dispensing mechanism dispensing a reagent into the unit on the reaction disk; and a spectrophotometer measuring light transmitted from a first light source and from the reaction liquid contained in the unit on the reaction disk. A scattering photometer that measures light scattered by a reaction liquid contained in a unit on the reaction disk after being irradiated by a second light source; a control circuit that drives the reaction disk, the sample dispensing mechanism, and the reagent dispensing mechanism; and a data processing unit that executes a sample measurement program and controls the control circuit according to the sample measurement program, characterized in that... In the aforementioned sample analysis method, The sample dispensing mechanism dispenses the sample into the unit on the reaction plate; The reagent dispensing mechanism dispenses reagents into the unit; For the reaction solution of the sample and the reagent, the data processing unit obtains at least a second photometric value from the scattering photometer; The data processing unit determines whether quantitative analysis based on the second light intensity value at the predetermined first photometric point can be performed. If the data processing unit determines that quantitative analysis based on the second photometric value cannot be performed, the reagent dispensing mechanism will dispense additional reagent into the unit; and For the reaction solution to which the reagent has been added, the data processing unit obtains at least a first photometric value from the spectrophotometer and performs a quantitative analysis based on the first photometric value at the photometric point after the additional dispensing.

9. The sample analysis method according to claim 8, characterized in that, When the data processing unit determines that it is possible to perform quantitative analysis based on the second light intensity value, it performs quantitative analysis based on the second light intensity value at the photometer points before and after the first photometer point.

10. The sample analysis method according to claim 8, characterized in that, The data processing unit maintains a first calibration curve for quantitative analysis based on the first light intensity value and a second calibration curve for quantitative analysis based on the second light intensity value. The first calibration curve is generated based on the first photometric value obtained for standard solutions of different concentrations, under measurement conditions where quantitative analysis based on the second photometric value in the sample measurement procedure is deemed impossible. The second calibration curve is a calibration curve generated based on the second photometric value obtained for standard solutions of different concentrations, according to the measurement conditions determined to be suitable for quantitative analysis based on the second photometric value in the sample measurement procedure.

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