An experimental system for chronic stress in experimental small animals

Abstract

The present invention discloses an experimental system for treating chronic stress in experimental small animals, comprising an experimental box, the bottom of which is fixed with an automatically adjustable box tilt component, and the experimental box is provided with an automatically adjustable component for space crowding, an automatically adjustable component for day and night reversal, an automatically adjustable component for cooling and heating, an automatically adjustable component for strobe light, an automatic noise playback component, and an automatic release component for natural enemy odors, thereby achieving the functions of causing the experimental small animals in the experimental box to be stimulated by tilt, space crowding, day and night reversal, high temperature, cold, strobe light, noise, and natural enemy odors, and also achieving food and water deprivation. The stimulation applied by the present invention substantially does not require the participation of experimental personnel and can be realized in an automated and intelligent manner, saving a large amount of manpower and achieving high experimental efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of animal chronic stress experiments, in particular to an experimental system for chronic stress experiments on small animals. Background Art

[0002] Stress is a common physiological and psychological reaction in modern humans. While appropriate stress responses can help boost immunity and promote physical and mental health, excessive stress responses can suppress immune function and lead to physical or psychological illness. Elucidating the impact of stress on immune function and its mechanisms can help identify effective interventions and is crucial for disease prevention and treatment.

[0003] Chronic stress response is a form of stress that can occur in a variety of situations. During epidemics of highly contagious pathogens, the ongoing, long-term lockdowns impacted residents' physical and mental health, leading many to experience chronic stress. In large cities like Beijing, Shanghai, Guangzhou, and Shenzhen, many people live in cramped quarters for extended periods, creating a stressful environment that can easily cause chronic stress. Furthermore, for those working in extreme environments like submarines and space stations, the long-term effects of chronic stress on their health cannot be ignored. Studies have shown that the adverse health effects of submarine crew members are primarily positively correlated with work-related stress, which stems primarily from the unique nature of their work environment. The most significant factor is the adverse health effects of physical stimulation on submarine crew members. Physiological and psychological stress are often intertwined. Research has shown that psychological stress can lead to decreased immune function in both humans and animals, particularly in cellular immunity.

[0004] Model mice are an important component in the study of chronic stress. Common stress models include the Chronic Unpredictable Mild Stress (CUMS) model, the Learned Helplessness (LH) model, the Chronic Restraint Stress (CRS) model, and the Social Defeat (SD) model. The CUMS model is one of the most effective and widely used animal models of depression. It simulates the many unpredictable mild stimuli that people encounter in their daily lives, mainly physical stress. This model focuses on anhedonia, the most core symptom of depression. The stimuli used generally include: day and night reversal, food and water deprivation, cold, crowding, tilting, strobing, noise, and the smell of natural enemies. Generally, 1-2 random stimuli are applied every day to make the animals unpredictable, and the experimental modeling time is 3-4 weeks. A large number of studies have reported that the CUMS model has good face validity (can well induce anhedonia symptoms) and predictive validity (behavioral changes can be reversed by multiple antidepressants), and its induced behavior and neurochemical changes have good stability. However, the stimulation applied by this model mostly requires the experimenter to be highly involved. The experimenter personally applies different stimulations every day for 3-4 weeks. This artificial implementation consumes a large amount of manpower, and is intelligent and automated, and inefficient. And there is no existing chronic stress experimental equipment that can complete all stimulation experiments, causing the realization of this chronic stress experiment to be very troublesome. If experimental animals are changed into different devices to realize corresponding stress stimulation, this will cause final experimental result to be inaccurate. Based on this, the present invention designs a kind of experimental system for experimental small animal chronic stress. Summary of the Invention

[0005] The present invention aims to solve the problems and shortcomings of the prior art and provides an experimental system for chronic stress in experimental small animals.

[0006] The present invention solves the above technical problems through the following technical solutions:

[0007] The present invention provides an experimental system for chronic stress in experimental small animals, which is characterized in that it includes an experimental box with an opening on the front, an automatic box tilt adjustment component arranged at the bottom of the experimental box, and a controller, wherein the bottom of the automatic box tilt adjustment component is fixed on the experimental table, and the controller is placed on the experimental table;

[0008] A load-bearing partition is fixed to the lower part of the experimental box to divide the internal space of the experimental box into an upper cavity for carrying multiple experimental small animals and a lower cavity for placing a waste tray. A plurality of leakage holes are provided on the load-bearing partition to allow waste generated by the experimental small animals to leak into the waste tray through the leakage holes. An upper box door is hinged on the front side of the experimental box and corresponds to the position of the upper cavity. A lower box door is hinged on the front side of the experimental box and corresponds to the position of the lower cavity. The left side panel, right side panel, upper side panel, lower side panel and rear side panel of the experimental box are non-transparent, and the upper box door and lower box door are non-transparent. A left window is provided on the left side panel, a right window is provided on the right side panel, and an upper window is provided on the upper side panel;

[0009] The experimental box is provided with an automatic adjustable component for space congestion, and the automatic adjustable component for space congestion includes: a left transparent movable plate arranged on the inner side of the left plate and a right transparent movable plate arranged on the inner side of the right plate, the size of the left transparent movable plate is larger than the size of the left window, the size of the right transparent movable plate is larger than the size of the right window, and a plurality of air holes are provided on both the left and right transparent movable plates, the front and rear ends of the load-bearing partitions are respectively fixed with transverse slide rails, the front and rear ends of the inner walls of the upper side plates are respectively fixed with transverse limiting rails corresponding to the transverse slide rails, the front and rear ends of the top sides of the experimental box are respectively fixed with first rotating motors, the output shaft of each of the first rotating motors is fixed with a first transverse screw rod, and each of the first transverse screw rods is placed in a corresponding transverse limiting rail, and the screw thread direction of the middle left position of each first transverse screw rod is opposite to the screw thread direction of the middle right position, the nuts on the front and rear ends of the tops of the left and right transparent movable plates are sleeved on the corresponding first transverse screw rods, and the small pulleys on the front and rear ends of the bottoms are slid in the corresponding transverse slide rails;

[0010] The experimental box is provided with an automatic adjustable day and night reversal component, which includes: a left automatic shading component fixed to the outer side of the left side panel for shading the left window, a right automatic shading component fixed to the outer side of the right side panel for shading the right window, a top automatic shading component fixed to the top surface of the upper side panel for shading the upper window, a photosensor and an illumination lamp fixed to the inner wall of the upper side panel and placed between the left transparent movable panel and the right transparent movable panel;

[0011] On the inner wall of the upper side plate and between the left transparent movable plate and the right transparent movable plate, an automatic cooling and heating component, an automatic strobe component, an automatic noise playing component and an automatic natural enemy smell releasing component are fixed;

[0012] The controller is used to control the automatic adjustable box tilt component to adjust the experimental box to a tilted state when receiving a tilt stimulation instruction, so that the experimental animal in the experimental box is subjected to tilt stimulation, and control the automatic adjustable box tilt component to adjust the experimental box to reset when the tilt stimulation duration reaches a preset tilt stimulation duration;

[0013] The controller is further configured to control the automatically adjustable space crowding component to adjust the internal space of the experimental box to a crowded state upon receiving a space crowding stimulation instruction, specifically controlling each first rotary motor to rotate to drive the corresponding first transverse screw to rotate, and the nuts at both ends of each first transverse screw to move toward each other under the action of the screw threads, so as to drive the left transparent movable plate and the right transparent movable plate to move toward each other, so that the activity space of the experimental small animals in the experimental box becomes smaller, thereby causing the experimental small animals in the experimental box to be subjected to crowding stimulation, and controlling the automatically adjustable space crowding component to adjust the internal space of the experimental box to reset when the crowding stimulation duration reaches a preset crowding stimulation duration;

[0014] The controller is also used to determine whether it is daytime or nighttime using the light intensity signal detected by the photosensor when receiving the day-night reversal stimulation instruction, and to control the left shading automatic adjustable component to perform shading adjustment on the left window, the right shading automatic adjustable component to perform shading adjustment on the right window, and the top shading automatic adjustable component to perform shading adjustment on the upper window when it is daytime, so that the upper cavity where the experimental animal is located is in a dark state, and the experimental animal is stimulated by the day being reversed to nighttime, and to control the right shading automatic adjustable component, the left shading automatic adjustable component, and the top shading automatic adjustable component to reset when the day-night reversal stimulation duration reaches a preset day-night reversal stimulation duration; to control the lighting lamp to perform lighting adjustment when it is nighttime, so that the upper cavity where the experimental animal is located is in a bright state, and the experimental animal is stimulated by the night being reversed to daytime, and to control the lighting lamp to be turned off when the day-night reversal stimulation duration reaches a preset day-night reversal stimulation duration;

[0015] The controller is further configured to, upon receiving a high temperature stimulation instruction, control the automatic cooling and heating adjustable components to perform heating and adjustment until the temperature inside the experimental box reaches a preset high temperature, so that the experimental small animals in the experimental box are subjected to high temperature stimulation, and, when the high temperature stimulation duration reaches a preset high temperature stimulation duration, control the automatic cooling and heating adjustable components to perform cooling and adjustment until the temperature inside the experimental box drops to an appropriate temperature range; upon receiving a cold stimulation instruction, control the automatic cooling and heating adjustable components to perform cooling and adjustment until the temperature inside the experimental box reaches a preset cold temperature, so that the experimental small animals in the experimental box are subjected to cold stimulation, and, when the cold stimulation duration reaches a preset cold stimulation duration, control the automatic cooling and heating adjustable components to perform heating and adjustment until the temperature inside the experimental box rises to an appropriate temperature range;

[0016] The controller is further configured to control the automatic stroboscopic adjustable component to emit stroboscopic light at a set frequency upon receiving a stroboscopic stimulation instruction, and to control the automatic stroboscopic adjustable component to shut down when the stroboscopic stimulation duration reaches a preset stroboscopic stimulation duration;

[0017] The controller is further configured to control the automatic noise playing component to emit noise at a set volume upon receiving a noise stimulation instruction, and to control the automatic noise playing component to shut down when the noise stimulation duration reaches a preset noise stimulation duration;

[0018] The controller is also used to control the natural enemy odor automatic release component to release the natural enemy odor at a set flow rate when receiving a natural enemy odor stimulation instruction, and control the natural enemy odor automatic release component to close when the natural enemy odor stimulation duration reaches a preset natural enemy odor stimulation duration.

[0019] The positive progress effect of the present invention is:

[0020] The present invention innovatively designs an automatic adjustable component for cabinet tilt, an automatic adjustable component for space congestion, an automatic adjustable component for day and night reversal, an automatic adjustable component for cooling and heating, an automatic adjustable component for strobe, an automatic noise playing component and an automatic release component for natural enemy odor. The automatic adjustable component for cabinet tilt can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by tilt, the automatic adjustable component for space congestion can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by space congestion, the automatic adjustable component for day and night reversal can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by day and night reversal, the automatic adjustable component for cooling and heating can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by high temperature and cold, the automatic adjustable component for strobe can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by strobe, the automatic noise playing component can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by noise, and the automatic release component for natural enemy odor can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by the odor of natural enemies, thereby realizing the various stimulations mentioned in the background technology.

[0021] The stimulation applied by the present invention basically does not require the participation of experimenters and can be realized automatically and intelligently, saving a lot of manpower and improving the experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 A three-dimensional diagram of an experimental system for chronic stress in experimental small animals according to a preferred embodiment of the present invention.

[0023] Figure 2 and Figure 3 Schematic diagram of the internal structure of the experimental box according to a preferred embodiment of the present invention.

[0024] Figure 4 This is a structural diagram of an automatic adjustable cabinet tilt assembly according to a preferred embodiment of the present invention.

[0025] Figure 5 This is a schematic structural diagram of an automatically adjustable component for space congestion according to a preferred embodiment of the present invention.

[0026] Figure 6 This is a schematic structural diagram of the experimental box according to a preferred embodiment of the present invention. DETAILED DESCRIPTION

[0027] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0028] like Figure 1-6 As shown, this embodiment provides an experimental system for chronic stress in experimental small animals, which includes an experimental box 1 with an opening on the front, an automatically adjustable box tilt component arranged at the bottom of the experimental box 1, an automatically adjustable space crowding component arranged on the experimental box 1, an automatically adjustable day and night reversal component arranged on the experimental box 1, an automatically adjustable cooling and heating component arranged in the experimental box 1, an automatically adjustable strobe component arranged in the experimental box 1, an automatic noise playing component arranged in the experimental box 1, an automatic release component of natural enemy odor arranged on the experimental box 1, and a controller. The experimental box 1 is fixed to the experimental table through the bottom of the automatically adjustable box tilt component, and the controller is also placed on the experimental table (not shown in the figure).

[0029] In this embodiment, innovatively designed automatic adjustable components for cabinet tilt, automatic adjustable components for space congestion, automatic adjustable components for day and night reversal, automatic adjustable components for cooling and heating, automatic adjustable components for strobe, automatic noise playback components and automatic release components for natural enemy odors are provided. The automatic adjustable component for cabinet tilt can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by tilt, the automatic adjustable component for space congestion can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by space congestion, the automatic adjustable component for day and night reversal can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by day and night reversal, the automatic adjustable component for cooling and heating can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by high temperature and cold, the automatic adjustable component for strobe can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by strobe, the automatic noise playback component can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by noise, and the automatic release component for natural enemy odors can realize the function of causing the experimental animals in the experimental cabinet to be stimulated by the odors of natural enemies.

[0030] like Figure 1-3 、 Figure 6 As shown, a load-bearing partition 2 is fixed to the lower part of the experimental box 1 to divide the internal space of the experimental box 1 into an upper cavity 3 for carrying multiple experimental small animals and a lower cavity 4 for placing a waste tray 5. A plurality of leakage holes 6 are provided on the load-bearing partition 2 so that the waste generated by the experimental small animals can leak into the waste tray 5 through the leakage holes 6. An upper door 7 is hinged on the front side of the experimental box 1 and corresponds to the position of the upper cavity 3. A lower door 8 is hinged on the front side of the experimental box 1 and corresponds to the position of the lower cavity 4. The left side panel 11, right side panel 12, upper side panel 13, lower side panel 14 and rear side panel 15 of the experimental box 1 are non-transparent, the upper door 7 and the lower door 8 are non-transparent, a left window 16 is provided on the left side panel 11, a right window 17 is provided on the right side panel 12, and an upper window 18 is provided on the upper side panel 13.

[0031] In this embodiment, innovative doors are designed for the upper cavity 3 for carrying the experimental animals and the lower cavity 4 for placing the waste tray 5, namely the upper door 7 and the lower door 8. In this way, when taking out the waste tray 5, there is no need to open all the doors. Only the lower door 8 corresponding to the waste tray 5 needs to be opened, which reduces the impact of the opening operation of the lower door 8 corresponding to the waste tray 5 when taking out the waste tray 5 on the chronic stress experiment of the experimental animals.

[0032] like Figure 2 As shown, a high-definition camera 9 is fixed on the inner wall of the upper side plate 13 and placed between the left transparent movable plate 201 and the right transparent movable plate 202 .

[0033] like Figure 4As shown, the automatic adjustable box tilt assembly includes: a first base 101 and a second base 102 located on the left and right sides respectively, the first base 101 and the second base 102 are arranged longitudinally and parallel, the first base 101 and the second base 102 are in the shape of long strips, and the front and rear ends of the first base 101 and the second base 102 are provided with screw holes 103, and bolts are passed through the corresponding screw holes 103 and screwed to the test bench to fix the experimental system on the test bench. A first bottom right-angled triangular bracket 104 is fixed between the front ends of the first base 101 and the second base 102, and a second bottom right-angled triangular bracket 105 is fixed between the rear ends. The right angles of the first bottom right-angled triangular bracket 104 and the second bottom right-angled triangular bracket 105 are both fixed to the first base 101, and the right-side acute angles of the first bottom right-angled triangular bracket 104 and the second bottom right-angled triangular bracket 105 are both fixed to the second base 102. The tops of the first bottom right-angled triangular bracket 104 and the second bottom right-angled triangular bracket 105 are both flat, and the first bottom right-angled triangular bracket 104 and the second bottom right-angled triangular bracket 105 located on the second base 102 are flat. A longitudinal connecting rod 106 is fixed between the bottom right-angled triangle bracket 104 and the second bottom right-angled triangle bracket 105. A first bearing 107 and a second bearing 108 arranged at intervals are fixed on the longitudinal connecting rod 106 and located between the first bottom right-angled triangle bracket 104 and the second bottom right-angled triangle bracket 105. The outer ring of the first bearing 107 is fixed with a first top right-angled triangle bracket 109, and the outer ring of the second bearing 108 is fixed with a second top right-angled triangle bracket 110. The right angles of the first top right-angled triangle bracket 109 and the second top right-angled triangle bracket 110 are both located at At the top, a second rotating motor 111 is fixed at the middle position of the first base 101, and one end of a second horizontal screw rod 112 is fixed to the output end of the second rotating motor 111. A fixing block 113 is fixed at the middle position of the second base 102. A third bearing 114 is embedded in the inner side of the fixing block 113. The other end of the second horizontal screw rod 112 is inserted into the third bearing 114. A longitudinal moving rod 115 is sleeved on the second horizontal screw rod 112. The two ends of the longitudinal moving rod 115 are respectively fixed with a first vertical rod 116 and a second vertical rod 117. The first vertical rod 116 is fixed to the The top of the first right-angled triangle bracket 109 has a rounded end, and the top of the second vertical rod 117 also has a rounded end. The bottom of the first right-angled triangle bracket 109 has a first sliding groove along its length, and the first sliding groove has a cross-section that opens downward. The round end of the first vertical rod 116 is embedded in the first sliding groove. The bottom of the second right-angled triangle bracket 110 has a second sliding groove along its length, and the second sliding groove has a cross-section that opens downward. The round end of the second vertical rod 117 is embedded in the second sliding groove. The tops of the first right-angled triangle bracket 109 and the second right-angled triangle bracket 110 are fixed with the experimental box 1. In the initial state, the tops of the first right-angled triangle bracket 109, the second right-angled triangle bracket 110, the first right-angled triangle bracket 104, and the second right-angled triangle bracket 105 are flush, so that the experimental box 1 is placed horizontally.

[0034] like Figure 1 and Figure 5 As shown, the space congestion automatic adjustable component includes: a left transparent movable plate 201 arranged on the inner side of the left side plate 11 and a right transparent movable plate 202 arranged on the inner side of the right side plate 12, the size of the left transparent movable plate 201 is larger than the size of the left window 16, the size of the right transparent movable plate 202 is larger than the size of the right window 17, and a plurality of ventilation holes 203 are opened on the left transparent movable plate 201 and the right transparent movable plate 202. The front and rear ends of the load-bearing partition 2 are respectively fixed with transverse slide rails 204, and the front and rear ends of the inner wall of the upper side plate 13 are respectively fixed with transverse limit rails 20 corresponding to the transverse slide rails 204. 5. The front and rear ends of the top side of the experimental box 1 are respectively fixed with first rotary motors 206, and the output shaft of each first rotary motor 206 is fixed with a first horizontal screw rod 207. Each first horizontal screw rod 207 is placed in the corresponding horizontal limit track 205. The direction of the screw thread at the middle left position of each first horizontal screw rod 207 is opposite to the direction of the screw thread at the middle right position. The nuts on the front and rear ends of the top of the left transparent movable plate 201 and the right transparent movable plate 202 are sleeved on the corresponding first horizontal screw rod 207, and the small pulleys on the front and rear ends of the bottom are slid into the corresponding horizontal slide rails 204.

[0035] like Figure 1 、 Figure 2 、 Figure 3 and Figure 6 As shown, the day and night reversal automatic adjustable component includes: a left shading automatic adjustable component fixed on the outer side of the left side panel 11 for shading the left window 16, a right shading automatic adjustable component fixed on the outer side of the right side panel 12 for shading the right window 17, a top shading automatic adjustable component fixed on the top surface of the upper side panel 13 for shading the upper window 18, and a photosensor 301 and a lighting lamp 302 fixed on the inner wall of the upper side panel 13 and placed between the left transparent movable plate 201 and the right transparent movable plate 202.

[0036] Among them, the left shading automatic adjustable component includes a left vertical limiting rail 303 fixed on the outer side of the left side plate 11 and located at the front and rear sides of the left window 16, and a left longitudinal fixed shaft fixed on the outer side of the left side plate 11 and located at the top of the left window 16. A left rotating motor 304 is fixed directly below each left vertical limiting rail 303, and the output shaft of each left rotating motor 304 is fixed with a left vertical screw rod. Each left vertical screw rod is placed in the corresponding left vertical limiting rail 303, and a left longitudinal rotating shaft 305 is sleeved on the left longitudinal fixed shaft. A left volute spring (not shown in the figure) is fixedly connected between the left longitudinal fixed shaft and the left longitudinal rotating shaft 305. A left shading roller blind 306 is wound on the left longitudinal rotating shaft 305, and the front and rear ends of the bottom end of the left shading roller blind 306 are respectively screwed to the corresponding left vertical screw rods.

[0037] The right shading automatic adjustable component includes a right vertical limiting rail 307 fixed on the outer side of the right side plate 12 and located on the front and rear sides of the right window 17, and a right longitudinal fixed shaft fixed on the outer side of the right side plate 12 and located at the top of the right window 17. A right rotating motor 308 is fixed directly below each right vertical limiting rail 307, and the output shaft of each right rotating motor 308 is fixed with a right vertical screw rod. Each right vertical screw rod is placed in the corresponding right vertical limiting rail 307. A right longitudinal rotating shaft 309 is sleeved on the right longitudinal fixed shaft, and a right volute spring (not shown in the figure) is fixedly connected between the right longitudinal fixed shaft and the right longitudinal rotating shaft 309. A right shading roller blind 310 is wound on the right longitudinal rotating shaft 309, and the front and rear ends of the bottom end of the right shading roller blind 310 are respectively screwed to the corresponding right vertical screw rods.

[0038] The top shading automatic adjustable component includes an upper horizontal limit rail 311 fixed on the surface of the upper side plate 13 and located at the front and rear sides of the upper window 18, and an upper longitudinal fixed shaft fixed on the surface of the upper side plate 13 and located at the top of the upper window 18. An upper rotating motor 312 is fixed on the right side of each upper horizontal limit rail 311, and the output shaft of each upper rotating motor 312 is fixed to an upper horizontal screw rod. Each upper horizontal screw rod is placed in the corresponding upper horizontal limit rail 311, and an upper longitudinal rotating shaft 313 is sleeved on the upper longitudinal fixed shaft. An upper volute spring (not shown in the figure) is fixedly connected between the upper longitudinal fixed shaft and the upper longitudinal rotating shaft 313. An upper shading roller curtain 314 is wound on the upper longitudinal rotating shaft 313, and the front and rear ends of the bottom ends of the upper shading roller curtain 314 are screwed to the corresponding upper horizontal screw rods respectively.

[0039] like Figure 2 and Figure 3 As shown, the automatic adjustable cooling and heating components include a temperature sensor 401, a heater 402 and a refrigerator 403 fixed on the inner wall of the upper side plate 13 and placed between the left transparent movable plate 201 and the right transparent movable plate 202. The heater 402 and the refrigerator 403 both use existing heaters and refrigerators, such as a resistance wire heater.

[0040] like Figure 2 As shown, the automatic adjustable strobe component uses a strobe light, which is fixed on the inner wall of the upper side plate 13 and placed between the left transparent movable plate 201 and the right transparent movable plate 202. The strobe light and the lighting lamp 302 can use the same lamp, and the lighting lamp 302 can strobe at a set frequency.

[0041] like Figure 2 As shown, the noise automatic playing component adopts a speaker 501, which is fixed on the inner wall of the upper side plate 13 and placed between the left transparent movable plate 201 and the right transparent movable plate 202.

[0042] like Figure 3 As shown, the natural enemy odor automatic release component includes an air intake pipe 601 fixed on the inner wall of the upper side plate 13 and placed between the left transparent movable plate 201 and the right transparent movable plate 202. The air intake pipe 601 is externally connected to a natural enemy odor gas source, and an air intake electric valve is provided on the air intake pipe 601 and placed outside the experimental box 1.

[0043] like Figure 6 As shown, a food inlet 701 and a water inlet 702 are provided on the upper side panel 13, and a food carrying plate 703 and a water carrying plate 704 are fixed on the inner wall of the experimental box 1 and directly below the food inlet 701 and the water inlet 702. The food carrying plate 703 and the water carrying plate 704 are both located between the left transparent movable plate 201 and the right transparent movable plate 202, and the food inlet 701 and the water inlet 702 are respectively plugged.

[0044] The second rotating motor 111 , the first rotating motor 206 , the left rotating motor 304 , the right rotating motor 308 and the upper rotating motor 312 are all existing micro motors.

[0045] The following describes the specific functions of each component:

[0046] The controller is used to control the second rotating motor 111 to rotate when receiving the tilt stimulation instruction, driving the second horizontal screw rod 112 to rotate, so that the longitudinal moving rod 115 moves along the second horizontal screw rod 112, and the first vertical rod 116 and the second vertical rod 117 move accordingly. During the movement, the top of the first vertical rod 116 slides in the first slide groove to push the first top right-angled triangle bracket 109 upward to rotate around the longitudinal connecting rod 106, and the top of the second vertical rod 117 slides in the second slide groove to push the second top right-angled triangle bracket 110 upward to rotate around the longitudinal connecting rod 106, so as to adjust the experimental box 1 to a tilted state, so that the experimental small animal in the experimental box 1 is subjected to tilt stimulation, and when the tilt stimulation time reaches the preset tilt stimulation time, the second rotating motor 111 is controlled to rotate in the opposite direction to adjust the experimental box 1 to reset.

[0047] The controller is also used to control each first rotary motor 206 to rotate when receiving a space crowding stimulation instruction to drive the corresponding first transverse screw rod 207 to rotate. The nuts at both ends of each first transverse screw rod 207 move toward each other under the action of the screw threads to drive the left transparent movable plate 201 and the right transparent movable plate 202 to move toward each other, so that the activity space of the experimental animals in the experimental box 1 becomes smaller, thereby causing the experimental animals in the experimental box 1 to be subjected to crowding stimulation, and when the crowding stimulation duration reaches the preset crowding stimulation duration, the first rotary motor 206 is controlled to rotate in the opposite direction to drive the left transparent movable plate 201 and the right transparent movable plate 202 to move away in opposite directions to adjust the internal space of the experimental box 1 to reset.

[0048] The controller is also used to use the light intensity signal detected by the photosensor 301 to determine whether it is daytime or nighttime when receiving the day / night reversal stimulation instruction, and control the left shading automatic adjustable component to adjust the shading of the left window 16, the right shading automatic adjustable component to adjust the shading of the right window 17, and the top shading automatic adjustable component to adjust the shading of the upper window 18 when it is daytime, so that the upper cavity 3 where the experimental animal is located is in a dark state, and the experimental animal is stimulated by the day being reversed to nighttime, and control the right shading automatic adjustable component, the left shading automatic adjustable component and the top shading automatic adjustable component to reset when the day / night reversal stimulation duration reaches the preset day / night reversal stimulation duration; control the lighting lamp 302 to adjust the lighting when it is nighttime, so that the upper cavity 3 where the experimental animal is located is in a bright state, and the experimental animal is stimulated by the night being reversed to daytime, and control the lighting lamp 302 to be extinguished when the day / night reversal stimulation duration reaches the preset day / night reversal stimulation duration.

[0049] The controller controls the left shading automatic adjustable component to adjust the shading of the left window 16, the right shading automatic adjustable component to adjust the shading of the right window 17, and the top shading automatic adjustable component to adjust the shading of the upper window 18. The specific implementation method is as follows: the controller controls each left rotating motor 304 to rotate, drives the left vertical screw rod to rotate, and the bottom end of the left shading roller curtain 306 moves downward accordingly, and the left vortex spring produces bending elastic deformation to adjust the shading of the left window 16; controls each right rotating motor 308 to rotate, drives the right vertical screw rod to rotate, and the bottom end of the right shading roller curtain 310 moves downward accordingly, and the right vortex spring produces bending elastic deformation to adjust the shading of the right window 17; controls each upper rotating motor 312 to rotate, drives the upper horizontal screw rod to rotate, and the bottom end of the upper shading roller curtain 314 moves to the right accordingly, and the upper vortex spring produces bending elastic deformation. The upper window 18 is adjusted to shading so that the upper cavity 3 where the experimental animals are located is in a dark state, and the experimental animals are stimulated by the reversal of day and night to night. When the duration of the day and night reversal stimulation reaches the preset duration of the day and night reversal stimulation, the left rotating motor 304 is controlled to rotate in reverse, and the bottom end of the left light-shielding roller curtain 306 moves upward accordingly. The left light-shielding roller curtain 306 is reset and rolled on the left longitudinal rotating shaft 305 under the action of the restoring force of the left vortex spring. The right rotating motor 308 is controlled to rotate in reverse, and the bottom end of the right light-shielding roller curtain 310 moves upward accordingly. The right light-shielding roller curtain 310 is reset and rolled on the right longitudinal rotating shaft 309 under the action of the restoring force of the right vortex spring. The upper rotating motor 312 is controlled to rotate in reverse, and the bottom end of the upper light-shielding roller curtain 314 moves left accordingly. The upper light-shielding roller curtain 314 is reset and rolled on the upper longitudinal rotating shaft 313 under the action of the restoring force of the upper vortex spring.

[0050] The controller is also used to use the temperature signal detected by the temperature sensor 401 to determine whether the temperature in the current experimental box 1 has reached the preset high temperature when receiving a high temperature stimulation instruction. If not, the controller controls the heater 402 to adjust the heating until the temperature in the experimental box 1 reaches the preset high temperature, so that the experimental animals in the experimental box 1 are subjected to high temperature stimulation, and when the high temperature stimulation duration reaches the preset high temperature stimulation duration, controls the refrigerator 403 to adjust the cooling until the temperature in the experimental box 1 drops to an appropriate temperature range.

[0051] The controller is also used to use the temperature signal detected by the temperature sensor 401 to determine whether the temperature in the current experimental box 1 has reached the preset cold temperature when receiving a cold stimulation instruction. If not, the controller controls the refrigerator 403 to cool and adjust until the temperature in the experimental box 1 reaches the preset cold temperature, so that the experimental animals in the experimental box 1 are subjected to cold stimulation. When the cold stimulation duration reaches the preset cold stimulation duration, the controller controls the heater 402 to heat and adjust until the temperature in the experimental box 1 rises to an appropriate temperature range.

[0052] The controller is also used to control the strobe light to emit strobe light at a set frequency when receiving a strobe stimulation instruction, and to control the strobe light to turn off when the strobe stimulation duration reaches a preset strobe stimulation duration.

[0053] The controller is further configured to control the speaker 501 to emit noise at a set volume upon receiving a noise stimulation instruction, and to control the speaker 501 to turn off when the noise stimulation duration reaches a preset noise stimulation duration.

[0054] The controller is used to control the air intake electric valve to set the opening to release the natural enemy odor when receiving the natural enemy odor stimulation instruction. The natural enemy odor enters the experimental box 1 through the air intake pipe 601, and controls the air intake electric valve to close when the natural enemy odor stimulation duration reaches the preset natural enemy odor stimulation duration.

[0055] During the entire experiment, the controller is used to record and display the start time of the chronic stress animal experiment when receiving the chronic stress animal experiment start instruction.

[0056] The controller is also used to record and display the end time of the chronic stress animal experiment when the chronic stress animal experiment reaches the set experimental time, and record and display the number of tilt stimulations and the duration of each stimulation, the timestamp of each start stimulation, the timestamp of each end stimulation, the stimulation situation of each experimental animal captured by the high-definition camera 9 during the entire experiment, the number of spatial crowding stimulations and the duration of each stimulation, the timestamp of each start stimulation, the timestamp of each end stimulation, the stimulation situation of each experimental animal captured by the high-definition camera 9, the number of day and night reversal stimulations and the duration of each stimulation, the timestamp of each start stimulation, the timestamp of each end stimulation, the number of high temperature stimulations and the duration of each stimulation, the timestamp of each start stimulation, the timestamp of each end stimulation, the timestamp of each high temperature stimulation 9, the number of cold stimulations and the duration of each stimulation, the timestamp of each stimulation start, the timestamp of each stimulation end, the situation of each stimulation of the experimental animals photographed by the high-definition camera 9, the number of stroboscopic stimulations and the duration of each stimulation, the timestamp of each stimulation start, the timestamp of each stimulation end, the situation of each stimulation of the experimental animals photographed by the high-definition camera 9, the number of noise stimulations and the duration of each stimulation, the timestamp of each stimulation start, the timestamp of each stimulation end, the situation of each stimulation of the experimental animals photographed by the high-definition camera 9, and the number of natural enemy odor stimulations and the duration of each stimulation, the timestamp of each stimulation start, the timestamp of each stimulation end, the situation of each stimulation of the experimental animals photographed by the high-definition camera 9.

[0057] To achieve the food deprivation and water deprivation functions, the experimenter does not add food to the food carrier 703 through the food inlet 701, nor does he add water to the water carrier 704 through the water inlet 702 for a period of time. When food and water need to be added, the experimenter removes the plugs on the food inlet 701 and the water inlet 702, and adds food to the food carrier 703 through the food inlet 701 and water to the water carrier 704 through the water inlet 702.

[0058] Although specific embodiments of the present invention have been described above, those skilled in the art will appreciate that these are merely illustrative and that the scope of the present invention is defined by the appended claims. Those skilled in the art may make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, and such changes and modifications are intended to fall within the scope of the present invention.

Claims

1. An experimental system for chronic stress in experimental small animals, characterized in that: The invention comprises an experimental box with an opening on the front, an automatic box tilt adjustment component arranged at the bottom of the experimental box, and a controller, wherein the bottom of the automatic box tilt adjustment component is fixed on the experimental table, and the controller is placed on the experimental table; A load-bearing partition is fixed to the lower part of the experimental box to divide the internal space of the experimental box into an upper cavity for carrying multiple experimental small animals and a lower cavity for placing a waste tray. A plurality of leakage holes are provided on the load-bearing partition to allow waste generated by the experimental small animals to leak into the waste tray through the leakage holes. An upper box door is hinged on the front side of the experimental box and corresponds to the position of the upper cavity. A lower box door is hinged on the front side of the experimental box and corresponds to the position of the lower cavity. The left side panel, right side panel, upper side panel, lower side panel and rear side panel of the experimental box are non-transparent, and the upper box door and lower box door are non-transparent. A left window is provided on the left side panel, a right window is provided on the right side panel, and an upper window is provided on the upper side panel; The experimental box is provided with an automatic adjustable component for space congestion, and the automatic adjustable component for space congestion includes: a left transparent movable plate arranged on the inner side of the left plate and a right transparent movable plate arranged on the inner side of the right plate, the size of the left transparent movable plate is larger than the size of the left window, the size of the right transparent movable plate is larger than the size of the right window, and a plurality of air holes are provided on both the left and right transparent movable plates, the front and rear ends of the load-bearing partitions are respectively fixed with transverse slide rails, the front and rear ends of the inner walls of the upper side plates are respectively fixed with transverse limiting rails corresponding to the transverse slide rails, the front and rear ends of the top sides of the experimental box are respectively fixed with first rotating motors, the output shaft of each of the first rotating motors is fixed with a first transverse screw rod, and each of the first transverse screw rods is placed in a corresponding transverse limiting rail, and the screw thread direction of the middle left position of each first transverse screw rod is opposite to the screw thread direction of the middle right position, the nuts on the front and rear ends of the tops of the left and right transparent movable plates are sleeved on the corresponding first transverse screw rods, and the small pulleys on the front and rear ends of the bottoms are slid in the corresponding transverse slide rails; The experimental box is provided with an automatic adjustable day and night reversal component, which includes: a left automatic shading component fixed to the outer side of the left side panel for shading the left window, a right automatic shading component fixed to the outer side of the right side panel for shading the right window, a top automatic shading component fixed to the top surface of the upper side panel for shading the upper window, a photosensor and an illumination lamp fixed to the inner wall of the upper side panel and placed between the left transparent movable panel and the right transparent movable panel; On the inner wall of the upper side plate and between the left transparent movable plate and the right transparent movable plate, an automatic cooling and heating component, an automatic strobe component, an automatic noise playing component and an automatic natural enemy smell releasing component are fixed; The controller is used to control the automatic adjustable box tilt component to adjust the experimental box to a tilted state when receiving a tilt stimulation instruction, so that the experimental animal in the experimental box is subjected to tilt stimulation, and control the automatic adjustable box tilt component to adjust the experimental box to reset when the tilt stimulation duration reaches a preset tilt stimulation duration; The controller is further configured to control the automatically adjustable space crowding component to adjust the internal space of the experimental box to a crowded state upon receiving a space crowding stimulation instruction, specifically controlling each first rotary motor to rotate to drive the corresponding first transverse screw to rotate, and the nuts at both ends of each first transverse screw to move toward each other under the action of the screw threads, so as to drive the left transparent movable plate and the right transparent movable plate to move toward each other, so that the activity space of the experimental small animals in the experimental box becomes smaller, thereby causing the experimental small animals in the experimental box to be subjected to crowding stimulation, and controlling the automatically adjustable space crowding component to adjust the internal space of the experimental box to reset when the crowding stimulation duration reaches a preset crowding stimulation duration; The controller is also used to determine whether it is daytime or nighttime using the light intensity signal detected by the photosensor when receiving the day-night reversal stimulation instruction, and to control the left shading automatic adjustable component to perform shading adjustment on the left window, the right shading automatic adjustable component to perform shading adjustment on the right window, and the top shading automatic adjustable component to perform shading adjustment on the upper window when it is daytime, so that the upper cavity where the experimental animal is located is in a dark state, and the experimental animal is stimulated by the day being reversed to nighttime, and to control the right shading automatic adjustable component, the left shading automatic adjustable component, and the top shading automatic adjustable component to reset when the day-night reversal stimulation duration reaches a preset day-night reversal stimulation duration; to control the lighting lamp to perform lighting adjustment when it is nighttime, so that the upper cavity where the experimental animal is located is in a bright state, and the experimental animal is stimulated by the night being reversed to daytime, and to control the lighting lamp to be turned off when the day-night reversal stimulation duration reaches a preset day-night reversal stimulation duration; The controller is further configured to, upon receiving a high temperature stimulation instruction, control the automatic cooling and heating adjustable components to perform heating and adjustment until the temperature inside the experimental box reaches a preset high temperature, so that the experimental small animals in the experimental box are subjected to high temperature stimulation, and, when the high temperature stimulation duration reaches a preset high temperature stimulation duration, control the automatic cooling and heating adjustable components to perform cooling and adjustment until the temperature inside the experimental box drops to an appropriate temperature range; upon receiving a cold stimulation instruction, control the automatic cooling and heating adjustable components to perform cooling and adjustment until the temperature inside the experimental box reaches a preset cold temperature, so that the experimental small animals in the experimental box are subjected to cold stimulation, and, when the cold stimulation duration reaches a preset cold stimulation duration, control the automatic cooling and heating adjustable components to perform heating and adjustment until the temperature inside the experimental box rises to an appropriate temperature range; The controller is further configured to control the automatic stroboscopic adjustable component to emit stroboscopic light at a set frequency upon receiving a stroboscopic stimulation instruction, and to control the automatic stroboscopic adjustable component to shut down when the stroboscopic stimulation duration reaches a preset stroboscopic stimulation duration; The controller is further configured to control the automatic noise playing component to emit noise at a set volume upon receiving a noise stimulation instruction, and to control the automatic noise playing component to shut down when the noise stimulation duration reaches a preset noise stimulation duration; The controller is further configured to control the natural enemy odor automatic release component to release the natural enemy odor at a set flow rate upon receiving a natural enemy odor stimulation instruction, and control the natural enemy odor automatic release component to shut down when the natural enemy odor stimulation duration reaches a preset natural enemy odor stimulation duration; The automatic adjustable box tilt assembly includes a first base and a second base arranged longitudinally on the left and right sides respectively, the first base and the second base are both fixed to the test bench, a first bottom right-angled triangle bracket is fixed between the front ends of the first base and the second base, a second bottom right-angled triangle bracket is fixed between the rear ends, the right-angled parts of the first bottom right-angled triangle bracket and the second bottom right-angled triangle bracket are both fixed on the first base, the right acute angle parts are both fixed on the second base, and the tops are both flat, a longitudinal connecting rod is fixed between the first bottom right-angled triangle bracket and the second bottom right-angled triangle bracket located on the second base, a first bearing and a second bearing arranged at intervals are fixed on the longitudinal connecting rod and between the first bottom right-angled triangle bracket and the second bottom right-angled triangle bracket, the first bearing is fixed on the first bearing, the second bearing is fixed on the second bearing, the right-angled parts of the first top right-angled triangle bracket and the second top right-angled triangle bracket are both located at the top, the first The cam is fixedly provided with a first end in the middle of the base, and the second end in the middle of the base is fixedly provided with a second end in the middle of the base, and the cam is fixedly provided with a second end in the middle of the base, and the cam is fixedly provided with a third bearing. The controller is used to control the second rotating motor to rotate when receiving a tilt stimulation instruction, driving the second horizontal screw rod to rotate, so that the longitudinal moving rod moves along the second horizontal screw rod, and the first vertical rod and the second vertical rod move accordingly. During the movement, the top of the first vertical rod slides in the first slide groove to push the first top right-angled triangle bracket upward and rotate around the longitudinal connecting rod, and the top of the second vertical rod slides in the second slide groove to push the second top right-angled triangle bracket upward and rotate around the longitudinal connecting rod to adjust the experimental box to a tilted state, so that the experimental small animal in the experimental box is subjected to tilt stimulation, and when the tilt stimulation time reaches a preset tilt stimulation time, the second rotating motor is controlled to rotate in the opposite direction to adjust the experimental box to reset.

2. The experimental system for chronic stress in experimental small animals according to claim 1, characterized in that: A high-definition camera is fixed on the inner wall of the upper side plate and placed between the left transparent movable plate and the right transparent movable plate; The controller is used to record and display the start time of the chronic stress animal experiment when receiving the chronic stress animal experiment start instruction; The controller is also used to record and display the end time of the chronic stress animal experiment when the chronic stress animal experiment reaches the set experimental time, and record and display the number of tilt stimulations and the duration of each stimulation, the time stamp of each start stimulation, the time stamp of each end stimulation, the stimulation situation of each experimental animal photographed by the high-definition camera during the entire experiment, the number of spatial crowding stimulations and the duration of each stimulation, the time stamp of each start stimulation, the time stamp of each end stimulation, the stimulation situation of each experimental animal photographed by the high-definition camera, the number of day and night reversal stimulations and the duration of each stimulation, the time stamp of each start stimulation, the time stamp of each end stimulation, the number of high temperature stimulations and the duration of each stimulation, the time stamp of each start stimulation, the time stamp of each end stimulation, the high-definition camera The conditions of each stimulation of the experimental animals captured by the camera, the number of cold stimulations and the duration of each stimulation, the timestamp of each stimulation start, the timestamp of each stimulation end, the conditions of each stimulation of the experimental animals captured by the high-definition camera, the number of stroboscopic stimulations and the duration of each stimulation, the timestamp of each stimulation start, the timestamp of each stimulation end, the conditions of each stimulation of the experimental animals captured by the high-definition camera, the number of noise stimulations and the duration of each stimulation, the timestamp of each stimulation start, the timestamp of each stimulation end, the conditions of each stimulation of the experimental animals captured by the high-definition camera, and the number of natural enemy odor stimulations and the duration of each stimulation, the timestamp of each stimulation start, the timestamp of each stimulation end, the conditions of each stimulation of the experimental animals captured by the high-definition camera.

3. The experimental system for chronic stress in experimental small animals according to claim 1, characterized in that: The first slide groove has a C-shaped cross-section with an open downward direction, and the second slide groove has a C-shaped cross-section with an open downward direction. The top of the first vertical rod is a round end, and the round end of the first vertical rod is embedded in the first slide groove. The top of the second vertical rod is a round end, and the round end of the second vertical rod is embedded in the second slide groove.

4. The experimental system for chronic stress in experimental small animals according to claim 1, characterized in that: The front and rear ends of the first base and the second base are both provided with screw holes, and bolts are passed through the corresponding screw holes and screwed to the test bench to fix the experimental system on the test bench.

5. The experimental system for chronic stress in experimental small animals according to claim 1, characterized in that: The left shading automatic adjustable component includes a left vertical limiting rail fixed on the outer side of the left panel and located at the front and rear sides of the left window respectively, and a left longitudinal fixed shaft fixed on the outer side of the left panel and located at the top of the left window, a left rotating motor is fixed directly below each of the left vertical limiting rails, an output shaft of each of the left rotating motors is fixed with a left vertical screw rod, and each of the left vertical screw rods is placed in the corresponding left vertical limiting rail; a left longitudinal fixed shaft is sleeved with a left longitudinal rotating shaft, a left volute spring is fixedly connected between the left longitudinal fixed shaft and the left longitudinal rotating shaft, a left shading roller blind is wound on the left longitudinal rotating shaft, and the front and rear ends of the bottom ends of the left shading roller blind are respectively screwed with the corresponding left vertical screw rods; The right shading automatic adjustable component includes a right vertical limiting rail fixed on the outer side of the right side panel and located at the front and rear sides of the right window respectively, and a right longitudinal fixed shaft fixed on the outer side of the right side panel and located at the top of the right window, a right rotating motor is fixed directly below each of the right vertical limiting rails, and the output shaft of each of the right rotating motors is fixed with a right vertical screw rod, and each of the right vertical screw rods is placed in the corresponding right vertical limiting rail; the right longitudinal fixed shaft is sleeved with a right longitudinal rotating shaft, and a right volute spring is fixedly connected between the right longitudinal fixed shaft and the right longitudinal rotating shaft, and a right shading roller blind is wound on the right longitudinal rotating shaft, and the front and rear ends of the bottom ends of the right shading roller blind are respectively screwed with the corresponding right vertical screw rods; The top shading automatic adjustable component includes upper horizontal limit rails fixed on the surface of the upper side plate and located at the front and rear sides of the upper window respectively, and an upper longitudinal fixed shaft fixed on the surface of the upper side plate and located at the top of the upper window, an upper rotating motor is fixed on the right side of each of the upper horizontal limit rails, an upper horizontal screw rod is fixed on the output shaft of each of the upper rotating motors, and each of the upper horizontal screw rods is placed in the corresponding upper horizontal limit rails; an upper longitudinal fixed shaft is sleeved with an upper longitudinal rotating shaft, and an upper vortex spring is fixedly connected between the upper longitudinal fixed shaft and the upper longitudinal rotating shaft, and an upper shading roller blind is wound on the upper longitudinal rotating shaft, and the front and rear ends of the bottom ends of the upper shading roller blind are respectively screwed with the corresponding upper horizontal screw rods; The controller is used to determine whether it is daytime or nighttime using the light intensity signal detected by the photosensor when receiving the day-night reversal stimulation instruction, and to control each left-rotating motor to rotate when it is daytime, so as to drive the left vertical screw rod to rotate, and the bottom end of the left light-shielding roller blind moves downward accordingly, and the left vortex spring produces bending elastic deformation to adjust the light-shielding of the left window, and to control each right-rotating motor to rotate, so as to drive the right vertical screw rod to rotate, and the bottom end of the right light-shielding roller blind moves downward accordingly, and the right vortex spring produces bending elastic deformation to adjust the light-shielding of the right window, and to control each upper-rotating motor to rotate, so as to drive the upper horizontal screw rod to rotate, and the bottom end of the upper light-shielding roller blind moves to the right accordingly, and the upper vortex spring produces bending elastic deformation to adjust the light-shielding of the upper window. Perform shading adjustment to make the upper cavity where the experimental animals are located in a dark state, and the experimental animals are stimulated by the day being reversed to night, and when the day and night reversal stimulation duration reaches the preset day and night reversal stimulation duration, the left rotating motor is controlled to rotate in reverse, and the bottom end of the left light-shielding roller blind moves upward, and the left light-shielding roller blind is reset and rolled on the left longitudinal rotating shaft under the restoring force of the left vortex spring, the right rotating motor is controlled to rotate in reverse, and the bottom end of the right light-shielding roller blind moves upward, and the right light-shielding roller blind is reset and rolled on the right longitudinal rotating shaft under the restoring force of the right vortex spring, the upper rotating motor is controlled to rotate in reverse, and the bottom end of the upper light-shielding roller blind moves to the left, and the upper light-shielding roller blind is reset and rolled on the upper longitudinal rotating shaft under the restoring force of the upper vortex spring.

6. The experimental system for chronic stress in experimental small animals according to claim 1, characterized in that: The automatic adjustable cooling and heating assembly includes a temperature sensor, a heater, and a refrigerator fixed on the inner wall of the upper side plate and placed between the left transparent movable plate and the right transparent movable plate; The controller is used to use the temperature signal detected by the temperature sensor to determine whether the temperature in the current experimental box has reached a preset high temperature when receiving a high-temperature stimulation instruction, and to control the heater to adjust the heating until the temperature in the experimental box reaches the preset high temperature when the temperature is not, so that the experimental animals in the experimental box are stimulated by high temperature, and to control the refrigerator to adjust the cooling when the high-temperature stimulation duration reaches the preset high-temperature stimulation duration until the temperature in the experimental box drops to an appropriate temperature range; and to use the temperature signal detected by the temperature sensor to determine whether the temperature in the current experimental box has reached a preset cold temperature when receiving a cold stimulation instruction, and to control the refrigerator to adjust the cooling when the temperature is not, so that the experimental animals in the experimental box are stimulated by cold, and to control the heater to adjust the heating when the cold stimulation duration reaches the preset cold stimulation duration until the temperature in the experimental box rises to an appropriate temperature range.

7. The experimental system for chronic stress in experimental small animals according to claim 1, characterized in that: The automatic adjustable strobe component adopts a strobe light, and the automatic noise playing component adopts a loudspeaker.

8. The experimental system for chronic stress in experimental small animals according to claim 1, characterized in that: The natural enemy odor automatic release component includes an air intake pipe fixed on the inner wall of the upper side plate and placed between the left transparent movable plate and the right transparent movable plate, the air intake pipe is externally connected to the natural enemy odor gas source, and an air intake electric valve is provided on the air intake pipe and placed outside the experimental box; The controller is used to control the air intake electric valve to release the natural enemy odor at a set opening when receiving a natural enemy odor stimulation instruction. The natural enemy odor enters the experimental box through the air intake pipe, and controls the air intake electric valve to close when the natural enemy odor stimulation duration reaches a preset natural enemy odor stimulation duration.

9. The experimental system for chronic stress in experimental small animals according to claim 1, characterized in that: A food inlet and a water inlet are provided on the upper side panel, and a food carrying plate and a water carrying plate are fixed on the inner wall of the experimental box and directly below the food inlet and the water inlet. The food carrying plate and the water carrying plate are both located between the left transparent movable plate and the right transparent movable plate, and the food inlet and the water inlet are respectively plugged.

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