A bird's nest composition capable of reducing the sensitization of cosmetic ingredients

Abstract

The present application belongs to the technical field of cosmetics, and particularly relates to a bird's nest composition capable of reducing the sensitization of cosmetic ingredients, which is composed of the following components in parts by weight: 30-60 parts of bird's nest acid, 10-30 parts of bird's nest peptide, 10-20 parts of bird's nest extract, and 1-30 parts of hydrolyzed bird's nest. The present application is derived from natural bird's nest and has good moisturizing and anti-allergic effects, and can effectively reduce the allergy caused by irritating substances in cosmetics, and solve the problem of skin sensitization caused by irritating ingredients in cosmetics.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a bird's nest composition capable of reducing the sensitization of cosmetic ingredients. BACKGROUND

[0002] Any skin type may produce various skin intolerance reactions under external stimulation, resulting in damage to the stratum corneum and stratum corneum, and thus becoming sensitive skin. Sensitive skin is an unhealthy skin type, which is manifested as burning, redness, and easy production of red blood cells on the face.

[0003] The domestic functional skin care market has maintained a steady growth trend in recent years. Compared with many chemical synthetic raw materials, raw materials derived from nature and having significant effects are more popular with consumers. With people's increasingly high demand for skin care, various skin care cosmetics have emerged in an endless stream. There are some troubles caused by the use of irritating ingredients, such as skin intolerance and allergy. SUMMARY

[0004] The purpose of the present application is to provide a bird's nest composition derived from natural bird's nest, which can reduce the sensitization of cosmetic ingredients.

[0005] To achieve the above purpose, the present application provides the following technical scheme:

[0006] A bird's nest composition capable of reducing the sensitization of cosmetic ingredients, which is composed of the following components in parts by weight: 30-60 parts of bird's nest acid, 10-30 parts of bird's nest peptide, 10-20 parts of bird's nest extract, and 1-30 parts of hydrolyzed bird's nest.

[0007] Further, the bird's nest acid is prepared from bird's nest powder, glucose and corn syrup dry powder as raw materials, through Escherichia coli fermentation, sterilization, filtration, freeze-drying and purification.

[0008] Further, the bird's nest peptide is prepared from bird's nest as raw material, through cleaning, stewing, grinding, homogenizing, pH adjustment, enzymolysis, enzyme inactivation, cooling, centrifugation, ultrafiltration, concentration and spray drying.

[0009] Further, the bird's nest extract is prepared from bird's nest as raw material, through crushing, enzymolysis, enzyme inactivation, cooling, centrifugation and filtration.

[0010] Further, the hydrolyzed bird's nest is prepared from bird's nest as raw material, through crushing, cooking, filtration, freeze-drying and crushing.

[0011] The present application also includes the use of the bird's nest composition as described in any one of the above in cosmetics.

[0012] The application has the advantages that the application is derived from natural bird's nest, has good moisturizing and anti-allergy effects, and can effectively reduce the allergy caused by irritating substances in cosmetics, and solve the problem of skin sensitization caused by irritating ingredients in cosmetics. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 KeratinoSens TM Test result determination criteria.

[0014] Figure 2 KeratinoSens test result determination criteria. DETAILED DESCRIPTION

[0015] The technical solutions of the application will be further described below in combination with specific embodiments. It should be understood that the following embodiments are only illustrative and explanatory of the application, and should not be interpreted as limiting the scope of protection of the application. Any technology implemented based on the above description of the application is covered within the scope of protection intended by the application.

[0016] Sensitization experiment

[0017] The 3-out-of-2 principle integration strategy is adopted to select a single alternative experiment covering KE1 to KE3 processes. If the results of 2 out of 3 key event alternative experiments are positive, the material is determined to have sensitization; if the results of 2 out of 3 key event alternative experiments are negative, the material is determined to have no sensitization.

[0018] The first key event adopts the DPRA experiment. When the test substance does not co-elute with cysteine polypeptide and lysine polypeptide, the cysteine polypeptide and lysine polypeptide model is used for determination, and the determination rules are shown in Table 1 below. When the test substance only co-elutes with lysine polypeptide, the cysteine polypeptide model is used for determination, and the determination rules are shown in Table 2 below. When the test substance co-elutes with cysteine polypeptide and lysine polypeptide, the determination conclusion is "undecided".

[0019] Table 1 Cysteine polypeptide and lysine polypeptide determination model

[0020]

[0021]

[0022] Table 2 Cysteine polypeptide determination model

[0023]

[0024] The second key event adopts KeratinoSens TM test and LuSens test. KeratinoSensTM Results of the test are determined by reference to Figure 1 Results of the KeratinoSens test are determined by reference to Figure 2 where EC1.5 represents the concentration of the test substance at which the luciferase gene is induced to express 1.5-fold.

[0025] The third key event uses the h-CLAT test to measure the expression level of CD86 and CD54 cell surface markers to support the differentiation between skin sensitisers and non-sensitisers. For CD86 / CD54 expression measurement, each test chemical is tested at least twice independently to derive a single prediction (positive or negative). The h-CLAT prediction is considered positive if at least one of the following conditions is met in at least 2 or 3 independent runs, otherwise the h-CLAT prediction is considered negative:

[0026] a) RFI of CD86 is equal to or greater than 150% (cell viability > 50%) at at least one test concentration;

[0027] b) RFI of CD54 is equal to or greater than 200% (cell viability > 50%) at at least one test concentration.

[0028] Example 1

[0029] Two non-sensitising substances commonly found in cosmetics were selected for the sensitisation test. The results of the DPRA test for the two non-sensitising substances are shown in Table 3 below.

[0030] Table 3 Results of the DPRA test

[0031]

[0032] The results of the KeratinoSens test for the two non-sensitising substances are shown in Table 4 below. TM Table 4 Results of the KeratinoSens test

[0033] Table 4 Results of the KeratinoSens test TM

[0034] Test substance EC1.5 (pmol / L) IC50 (pmol / L) KeratinoSens TM predict Hydroxybenzyl ester >1000 >1000 Negative Glycerol >1000 >1000 Negative

[0035] The results of the LuSens test for the two non-sensitising substances are shown in Table 5 below.

[0036] Table 5 Results of the LuSens test

[0037] Test substance EC1.5 (pmol / L) CV75 / (pg / mL) LuSens prediction Hydroxybenzyl ester >1000 >2000 Negative Glycerol >1000 >2000 Negative

[0038] The results of the h-CLAT test for the two non-sensitising substances are shown in Table 6 below.

[0039] Table 6 Results of the h-CLAT test​

[0040]

[0041]

[0042] The above experimental results further verify that the sensitization experiment is correct in judging the sensitization of the substance, and the sensitization test can be carried out according to the experimental method.

[0043] Example 2

[0044] Preparation of bird's nest acid: good bird's nest is dried, then crushed to obtain powder, then the bird's nest powder is dissolved in deionized water, and glucose, corn syrup dry powder (bird's nest powder: glucose: corn syrup dry powder = 1:2:1.5) are added, stirred uniformly and heated to 35-40℃, the pH is adjusted to 7.4-7.5, and Escherichia coli obtained by soil screening is added and fermented for 24h. After 24h, the temperature is raised to 75-80℃, and sterilized for 5-10min. After cooling, centrifugation is performed at 12000rpm for 20min to obtain supernatant containing bird's nest acid, which is freeze-dried, washed with anhydrous ethanol and dried to obtain the finished product.

[0045] Preparation of bird's nest peptide: good bird's nest is washed, then cooked in a water bath at 85℃ for 2h, and then homogenized. The pH is adjusted to 5.0-7.0, trypsin is added, and hydrolysis is performed for 20min, then the enzyme is inactivated at high temperature. After cooling, centrifugation is performed at 12000rpm for 20min, and the supernatant is concentrated and dried to obtain the finished product.

[0046] Preparation of bird's nest extract: good bird's nest is washed, dried and crushed to obtain bird's nest powder. The bird's nest powder is dispersed in deionized water, the pH is adjusted to 5.0-7.0, trypsin is added, and hydrolysis is performed for 30min, then the enzyme is inactivated at high temperature. After cooling, centrifugation is performed at 12000rpm for 20min, and the finished product is obtained after filtration.

[0047] Preparation of hydrolyzed bird's nest: good bird's nest is washed to remove impurities such as floating hair, then stirred and crushed, cooked in a water bath at 90℃ for 4h, filtered, freeze-dried, and finally crushed to obtain the finished product in powder form.

[0048] According to the sensitization experiment, the prepared bird's nest acid, bird's nest peptide, bird's nest extract and hydrolyzed bird's nest are subjected to a sensitization experiment.

[0049] As shown in Table 7 below are the DPRA experimental results of the four substances.

[0050] Table 7 DPRA experimental results

[0051]

[0052]

[0053] The KeratinoSens results of the four substances are shown in Table 8 below. TM Experimental results.

[0054] Table 8 KeratinoSens results TM Experimental results

[0055] Test substance EC1.5 (pmol / L) IC50 (pmol / L) KeratinoSens TM predict Cordycepin >1000 >1000 Negative Cordy peptide >1000 >1000 Negative Cordy extract >1000 >1000 Negative Hydrolyzed bird's nest >1000 >1000 Negative

[0056] The LuSens experimental results of the four substances are shown in Table 9 below.

[0057] Table 9 LuSens experimental results

[0058] Test substance EC1.5 (pmol / L) CV75 / (pg / mL) LuSens prediction Cordycepin >1000 >2000 Negative Cordy peptide >1000 >2000 Negative Cordy extract >1000 >2000 Negative Hydrolyzed bird's nest >1000 >2000 Negative

[0059] The h-CLAT experimental results of the four substances are shown in Table 10 below.

[0060] Table 10 h-CLAT experimental results

[0061]

[0062]

[0063] According to the 4 key events of the skin sensitization AOP, the 3-2 rule integration strategy is adopted, combined with the experimental results of Tables 7-10, it can be determined that bird's nest acid, bird's nest peptide, bird's nest extract, and hydrolyzed bird's nest do not have sensitization.

[0064] Example 3

[0065] Take the bird's nest acid, bird's nest peptide, bird's nest extract and hydrolyzed bird's nest prepared in Example 2, and prepare a bird's nest composition according to 30-60 parts of bird's nest acid, 10-30 parts of bird's nest peptide, 10-20 parts of bird's nest extract and 1-30 parts of hydrolyzed bird's nest. The bird's nest composition is subjected to a sensitization experiment.

[0066] The DPRA experimental results of the bird's nest composition are shown in Table 11 below.

[0067] Table 11 DPRA experimental results of the bird's nest composition

[0068]

[0069] The KeratinoSens results of the bird's nest composition are shown in Table 12 below. TM Experimental results.

[0070] Table 12 KeratinoSens results of the bird's nest composition TM Experimental results

[0071] Test substance EC1.5 (pmol / L) IC50 (pmol / L) KeratinoSens TM predict Cordy composition >1000 >1000 Negative

[0072] The LuSens experimental results of the bird's nest composition are shown in Table 13 below.

[0073] Table 13 LuSens experimental results of the bird's nest composition

[0074] Test substance EC1.5 (pmol / L) CV75 / (pg / mL) LuSens prediction Cordy composition >1000 >2000 Negative

[0075] The h-CLAT experimental results of the bird's nest composition are shown in Table 14 below.

[0076] Table 14 h-CLAT experimental results of the bird's nest composition

[0077] Test substance CV75 / (pg / mL) CD86 EC150 CD54 EC200 h-CLAT prediction result Cordy composition >5000 >200 >5000 Negative

[0078] According to the 4 key events of the skin sensitization AOP, the 3 out of 2 principle integration strategy is adopted, combined with the experimental results of Tables 11-14, it can be determined that the bird's nest composition does not have sensitization.

[0079] Example 4

[0080] Several irritating substances were selected from common additives in cosmetics for sensitization experiments.

[0081] The DPRA experimental results of several irritating substances are shown in Table 15 below.

[0082] Table 15 DPRA experimental results of several irritating substances

[0083]

[0084] The KeratinoSens experimental results of several irritating substances are shown in Table 16 below. TM

[0085] Table 16 KeratinoSens experimental results of several irritating substances TM

[0086]

[0087]

[0088] The LuSens experimental results of several irritating substances are shown in Table 17 below.

[0089] Table 17 LuSens experimental results of several irritating substances

[0090] Test substance EC1.5 (pmol / L) CV75 / (pg / mL) LuSens prediction Resorcinol 1.80 5.69 Positive Imidazole alkyl urea 3.54 9.89 Positive Ethanolamine 24.83 31.11 Positive Oleamide propyl dimethyl amine 19.36 30.05 Positive Fruit acid 516.55 843.12 Positive

[0091] The h-CLAT experimental results of several irritating substances are shown in Table 18 below.​​

[0092] Table 18 h-CLAT experimental results of stimulatory substances

[0093] Test substance CV75 / (pg / mL) CD86 EC150 CD54 EC200 h-CLAT prediction result Resorcinol 24.90 8.64 13.40 Positive Imidazole alkyl urea 36.99 34.97 39.11 Positive Ethanolamine 55.75 / 71.30 Positive Oleamide propyl dimethyl amine 40.02 35.26 22.18 Positive Fruit acid 51.16 103.21 66.51 Positive

[0094] According to the four key events of skin sensitization AOP, combined with the experimental results of Table 15-Table 18, it can be determined that resorcinol, imidazole alkyl urea, ethanolamine, oleamide propyl dimethylamine and fruit acid have sensitization.

[0095] The composition of the above additives was subjected to sensitization experiment.

[0096] As shown in Table 19, the DPRA experimental results of several stimulatory substance compositions are shown in the following table 19.

[0097] Table 19 DPRA experimental results of stimulatory substance compositions

[0098]

[0099]

[0100] As shown in Table 20, the KeratinoSens experimental results of several stimulatory substance compositions are shown in the following table 20. TM Experimental results.

[0101] Table 20 KeratinoSens experimental results of stimulatory substance compositions TM Experimental results

[0102]

[0103] As shown in Table 21, the LuSens experimental results of several stimulatory substance compositions are shown in the following table 21.

[0104] Table 21 LuSens experimental results of stimulatory substance compositions

[0105]

[0106] As shown in Table 22, the h-CLAT experimental results of several stimulatory substance compositions are shown in the following table 22.

[0107] Table 22 h-CLAT experimental results of stimulatory substance compositions

[0108]

[0109] According to the four key events of skin sensitization AOP, combined with the experimental results of Table 19-Table 22, it can be determined that the composition of resorcinol, imidazole alkyl urea, ethanolamine, oleamide propyl dimethylamine and fruit acid has sensitization.

[0110] Example 5

[0111] The sensitizing test was performed by adding the bird's nest composition of Example 3 to the composition of the sensitizing substance of Example 4.

[0112] The DPRA test results of the bird's nest composition in combination with the skin irritating substance are shown in Table 23 below.

[0113] Table 23 DPRA test results of the bird's nest composition in combination with the skin irritating substance

[0114]

[0115] The KeratinoSens test results of the bird's nest composition in combination with the skin irritating substance are shown in Table 24 below. TM Experimental results.

[0116] Table 24 KeratinoSens test results of the bird's nest composition in combination with the skin irritating substance TM Experimental results

[0117]

[0118] The LuSens test results of the bird's nest composition in combination with the skin irritating substance are shown in Table 25 below.

[0119] Table 25 LuSens test results of the bird's nest composition in combination with the skin irritating substance

[0120]

[0121] The h-CLAT test results of the bird's nest composition in combination with the skin irritating substance are shown in Table 26 below.

[0122] Table 26 h-CLAT test results of the bird's nest composition in combination with the skin irritating substance

[0123]

[0124]

[0125] According to the 4 key events of the skin sensitization AOP, the 2-out-of-3 principle integration strategy was used, combined with the experimental results of Tables 23-26, it was determined that the bird's nest composition in combination with the skin irritating substance did not have sensitization.

[0126] Example 6

[0127] Moisturizing efficacy test of the bird's nest composition.

[0128] The safety concentration of the bird's nest composition of Example 3 was tested by MTT method, and the experimental results are shown in Table 23.

[0129] Table 23: Results of cell activity test of bird's nest composition

[0130]

[0131] When the cell viability reaches 80%, it is the safety concentration, that is, the tested sample has no inhibitory effect on the cells. As shown in Table 23, the cell viability of the bird's nest composition is more than 80% at a concentration of 0.5%, and the cell viability is more than 90% at a concentration of 0.1%, 0.05% and 0.01%.

[0132] The bird's nest composition at concentrations of 0.1%, 0.05% and 0.01% was selected for testing the expression amount of AQP3 gene, and the experimental results are shown in Table 24.

[0133] Table 24: Up-regulation rate of bird's nest composition on AQP3 gene expression amount

[0134]

[0135] The bird's nest composition at concentrations of 0.1%, 0.05% and 0.01% was selected for testing the expression amount of HAS3 gene, and the experimental results are shown in Table 25.

[0136] Table 25: Up-regulation rate of bird's nest composition on HAS3 gene expression amount

[0137]

[0138] As shown in Tables 24 and 25, the bird's nest composition can promote the expression of AQP3 and HAS3 genes, and achieve the moisturizing effect.

[0139] The above examples verify that the bird's nest composition of the present application, i.e., the composition of 30-60 parts of bird's nest acid, 10-30 parts of bird's nest peptide, 10-20 parts of bird's nest extract and 1-30 parts of hydrolyzed bird's nest, significantly reduces the sensitization of the combination of irritating substances such as resorcinol, imidazole alkyl urea, ethanolamine, oleamide propyl dimethylamine and fruit acid, improves the expression of aquaporin (AQP3), hyaluronic acid synthetase 3 (HAS3) and genes, and has excellent anti-allergy and moisturizing effects.

[0140] Although the specific embodiments of the present application are described above, those skilled in the art should understand that the specific examples described are only illustrative, and are not intended to limit the scope of the present application, and equivalent modifications and changes made by those skilled in the art in accordance with the spirit of the present application should be covered within the scope of the claims of the present application.

Claims

1. A bird's nest composition capable of reducing the sensitization of cosmetic ingredients, characterized in that: The composition comprises 30-60 parts of bird's nest acid, 10-30 parts of bird's nest peptide, 10-20 parts of bird's nest extract and 1-30 parts of hydrolyzed bird's nest by weight; ​ The bird's nest acid is prepared from bird's nest powder, glucose and corn syrup dry powder, and is prepared through fermentation of Escherichia coli, sterilization, filtration, freeze-drying and purification; The bird's nest peptide is prepared from bird's nest, and is prepared through cleaning, stewing, grinding, homogenizing, pH adjustment, trypsin enzymolysis, enzyme inactivation, cooling, centrifugation, ultrafiltration, concentration and spray drying; The bird's nest extract is prepared from bird's nest, and is prepared through crushing, trypsin enzymolysis, enzyme inactivation, cooling, centrifugation and filtration; The hydrolyzed bird's nest is prepared from bird's nest, and is prepared through crushing, stewing, filtration, freeze-drying and crushing.

2. Use of the bird's nest composition according to claim 1 in the preparation of cosmetics.

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