A method for evaluating the yield of asparagus

Abstract

The present application relates to the field of plant genetic breeding, and particularly relates to a kind of evaluation method of asparagus yield. Including the following steps: S1, obtaining young plant period asparagus;S2, measures i variety of young plant period asparagus following parameters: photosynthetic rate A, ribulose-1,5-bisphosphate carboxylase / oxygenase activity B, leaf dry weight per unit area C, acid invertase activity D and root crown ratio E;The measurement sample of the acid invertase activity D is the tender stem of young plant period asparagus at 10-30cm from top;S3, according to the formula, the evaluation value is calculated, and the evaluation value is used to evaluate the yield of asparagus. The evaluation value obtained by using the above measurement index and calculation formula, and the significance level obtained by SPSS software is used to evaluate the yield of asparagus. The yield potential of asparagus can be comprehensively evaluated from the genetic point of view in a short time. Using the evaluation method of the present application, high-yield asparagus varieties can be selected in a short time without tracking for many years.

Description

Technical Field

[0001] This invention relates to the field of plant genetics and breeding, specifically to a method for evaluating asparagus yield. Background Technology

[0002] Asparagus (Asparagus officinalis L.) is a perennial herbaceous plant belonging to the genus Asparagus in the family Asparagaceae. Its tender stems have a unique taste and are rich in nutrients, earning it the title of "King of Vegetables." Due to its abundant bioactive substances, it possesses health-promoting and disease-fighting properties and has been listed as an important vegetable with both medicinal and edible uses. Every autumn, as temperatures drop, the above-ground parts of the asparagus wither and die, while the underground rootstock enters a dormant state. The following spring, it utilizes the nutrients accumulated and stored in the roots from the previous year to support the sprouting of tender shoots, stem growth, and crown formation. It then uses photosynthesis in its leaves to produce organic matter, which is transported and stored in the underground roots to support the growth of tender shoots in the current and following year, thus creating a cyclical process. Asparagus leaves are divided into two types: true leaves and pseudo-leafs. True leaves are degenerate leaflets that grow on the nodes of the above-ground stem and appear as triangular, membranous scales. The pseudo-leaf is a modified branch, clustered and needle-like, consisting of 6-9 needle-shaped leaf-like branches differentiated from the leaf axils. Like leaves of other plants, the pseudo-leaf contains a large amount of chlorophyll and is the main organ for photosynthesis. The energy for the formation of asparagus shoots comes from photosynthetic products and sugars transported and stored in the roots. Therefore, source, sink, and flow characteristics all significantly affect asparagus yield.

[0003] Currently, yield remains one of the main objectives in asparagus breeding. Surveys on yield-related traits often rely on agronomical indicators such as the number and thickness of shoots. However, crop yield is closely related to its inherent genetic and physiological characteristics, and evaluation based solely on apparent agronomic traits is neither comprehensive nor accurate. Some reports use changes in fertilizer application to evaluate cotton yield, which falls under the category of cultivation management research. In vegetable breeding, however, uniform fertilizer and water management is typically implemented, relying on the genetic characteristics of both parents to screen for high-yielding combinations or lines. High yield is genetically determined, meaning that yield formation is determined by intrinsic physiological characteristics. Research on comparative methods for asparagus yield, both domestically and internationally, is limited. Evaluation primarily utilizes visual morphological indicators such as the number and thickness of stems, the weight of young shoots, and the weight of marketable shoots. This method requires at least three years of measurement data from mature plants for strain or combination yield differentiation screening, which is very time-consuming and slows down the breeding process.

[0004] Therefore, given the lack of a comprehensive method for evaluating asparagus yield in existing technologies and the long evaluation time required, there is an urgent need to find a new method for evaluating asparagus yield in a short period of time. Summary of the Invention

[0005] This invention addresses the problem of the lack of a comprehensive method for evaluating asparagus yield and the long evaluation time required in the prior art by providing a method for evaluating asparagus yield.

[0006] To achieve the above technical objectives, the present invention provides a method for evaluating asparagus yield, comprising the following steps:

[0007] S1. Obtain young asparagus plants;

[0008] S2. Measure the following parameters of young asparagus plants of i varieties: photosynthetic rate (A), ribulose-1,5-bisphosphate carboxylase / oxygenase (Rubisco enzyme) activity (B), leaf dry weight per unit area (C), acid invertase activity (D), and root-to-shoot ratio (E); the acid invertase activity (D) was measured from the tender stems of young asparagus plants 10-30 cm from the top.

[0009] S3. Calculate the evaluation value according to the following formula, and evaluate the asparagus yield based on the evaluation value. in, The sum of A values ​​for asparagus from the first variety to the i-th variety during the juvenile stage. The sum of the B values ​​for asparagus from the first variety to the i-th variety during the juvenile stage. The sum of C values ​​for asparagus from the first variety to the i-th variety during the juvenile stage. The sum of the D values ​​of asparagus seedlings from the first variety to the i-th variety during their juvenile stage. The sum of E values ​​for asparagus from the first variety to the i-th variety during the juvenile stage;

[0010] A i Let A be the value of asparagus in the juvenile stage of the i-th variety, and B be the value of B. i Let B be the value of the i-th variety of asparagus in its juvenile stage, and C be the value of the juvenile asparagus. i Let C be the value of the i-th variety of asparagus in its juvenile stage, and D be the value of the juvenile asparagus. i Let D be the value of the i-th variety of asparagus in its juvenile stage, and E be the value of the juvenile asparagus. i Let E be the value of the juvenile asparagus of the i-th variety.

[0011] In step S1, the asparagus seeds to be tested are germinated, sown, transplanted, and cultured to obtain the young asparagus plants.

[0012] The germination process includes soaking the asparagus seeds to be tested in water for 30-36 hours, and then placing the seeds in an environment with a temperature of 25-28℃ for 2-3 days to germinate; preferably, the process also includes disinfecting the asparagus seeds to be tested with disinfectant before soaking.

[0013] The sowing process involves sowing the germinated seeds (which have sprouted white seeds) in nutrient soil and cultivating them for 43-48 days, followed by continued cultivation for 12-13 months after transplanting.

[0014] The sample used to measure the photosynthetic rate A is a pseudo-leaf of asparagus in the juvenile stage; preferably, it is a pseudo-leaf from the upper part of the juvenile asparagus plant.

[0015] The measurement time range of the photosynthetic rate A is 10-16 points; preferably, the measurement time range of the photosynthetic rate A is 12-14 points.

[0016] The sample used to measure the activity B of ribulose-1,5-bisphosphate carboxylase / oxygenase was a pseudo-leaf of young asparagus plants; preferably, a pseudo-leaf from the upper part of the young asparagus plant.

[0017] The sample for measuring the leaf dry weight C per unit area is a pseudo-leaf of young asparagus plants after drying treatment; preferably, it is a pseudo-leaf from the upper part of the young asparagus plant.

[0018] The activity D of the acid invertase was measured in young asparagus stems 12.5-17.5 cm from the top.

[0019] The root-to-shoot ratio E is the dry matter weight ratio of the underground and above-ground parts of asparagus during the young plant stage.

[0020] In this invention, the upper part of the plant refers to the portion from 1 / 3 of the distance from the top of the plant to 1 / 2 of the distance from the top of the plant.

[0021] In this invention, the evaluation of asparagus yield refers to judging the yield of two or more asparagus varieties, rather than quantitatively measuring the yield of a single asparagus variety.

[0022] This invention has found that asparagus yield is positively correlated with the evaluation value. By comparing the evaluation values, the yield of two or more asparagus varieties can be determined.

[0023] The i varieties of asparagus may include at least one variety of asparagus to be tested and at least one variety of control asparagus.

[0024] If the assessed value of the asparagus being tested is greater than that of the control asparagus, then the yield of the asparagus being tested is greater than that of the control asparagus. Conversely, if the assessed value of the asparagus being tested is less than that of the control asparagus, then the yield of the asparagus being tested is less than that of the control asparagus.

[0025] If the assessed value of the asparagus to be tested is equal to the assessed value of the control asparagus, then the yields of the asparagus to be tested and the control asparagus are the same. The control asparagus is asparagus with a known yield. The asparagus to be tested is asparagus with an unknown yield.

[0026] The young asparagus plants of i varieties, i≥2; for example, it can be 1 asparagus plant to be tested and 1 control asparagus plant; or it can be 2, 6 or 10 asparagus plants to be tested and 1 control asparagus plant.

[0027] The technical solution of the present invention has the following beneficial effects:

[0028] (1) The present invention provides a method for evaluating asparagus yield, comprising the following steps: S1, obtaining young asparagus plants; S2, measuring the following parameters of young asparagus plants of i varieties: photosynthetic rate A, ribulose-1,5-bisphosphate carboxylase / oxygenase activity B, leaf dry weight per unit area C, acid invertase activity D, and root-to-shoot ratio E; wherein the sample for measuring acid invertase activity D is the tender stem of young asparagus plants 10-30 cm from the top; S3, calculating the evaluation value according to the following formula, and evaluating the asparagus yield based on the evaluation value. in, The sum of A values ​​for asparagus from the first variety to the i-th variety during the juvenile stage. The sum of the B values ​​for asparagus from the first variety to the i-th variety during the juvenile stage. The sum of C values ​​for asparagus from the first variety to the i-th variety during the juvenile stage. The sum of the D values ​​of asparagus seedlings from the first variety to the i-th variety during their juvenile stage. A is the sum of the E values ​​of asparagus from the first variety to the i-th variety during the juvenile stage; i Let A be the value of asparagus in the juvenile stage of the i-th variety, and B be the value of B. i Let B be the value of the i-th variety of asparagus in its juvenile stage, and C be the value of the juvenile asparagus. i Let C be the value of the i-th variety of asparagus in its juvenile stage, and D be the value of the juvenile asparagus. i Let D be the value of the i-th variety of asparagus in its juvenile stage, and E be the value of the juvenile asparagus. iLet E be the yield value of the juvenile asparagus of the i-th variety. This invention evaluates asparagus yield by considering three aspects: photosynthetic capacity, material translocation, and energy storage. It employs five specific measurement indicators and calculates an evaluation value using a formula. The evaluation value is positively correlated with yield; a higher evaluation value corresponds to a higher yield, and a lower evaluation value corresponds to a lower yield. This provides a comprehensive method for evaluating asparagus yield, allowing for accurate yield assessment in a shorter time. This invention considers three aspects—photosynthetic capacity, material translocation, and energy storage—namely, source, flow, and sink, using a comprehensive evaluation method to assess asparagus yield. This saves time and labor, eliminating the need to track agronomic parameters for many years, and allows for the selection of high-yielding asparagus strains or combinations, providing a reference for selecting yield traits in asparagus variety breeding. Specifically, this invention uses photosynthetic rate and Rubisco enzyme activity as indicators from the perspective of the "source," because there is a significant difference in the maximum photosynthetic rate achievable by high-yielding and low-yielding asparagus varieties. This invention selects acidic invertase as a measurement indicator from the perspective of "flow" because this enzyme determines the differences in sucrose metabolism among varieties and has a significant impact on yield. This invention selects the root-to-shoot ratio as a measurement indicator from the perspective of "sink" because the root-to-shoot ratio has a significant impact on yield formation. In variety breeding, this invention allows for comparison of the yields of different lines or combinations obtained through hybridization using evaluation values. A control variety can be selected for yield comparison, and based on the comparison results, combinations or lines with high yields can be selected for further hybridization and other related work.

[0029] (2) The present invention provides a method for evaluating asparagus yield, which further improves the accuracy of evaluating asparagus yield by limiting the measurement samples and sampling locations of photosynthetic rate A, ribulose-1,5-bisphosphate carboxylase / oxygenase activity B, leaf dry weight per unit area C, and acid invertase activity D.

[0030] (3) The present invention provides a method for evaluating asparagus yield by limiting the measurement time range of photosynthetic rate A to 10-16 points; preferably, the measurement time range of photosynthetic rate A is 12-14 points. This can further improve the accuracy of asparagus yield evaluation. Attached Figure Description

[0031] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0032] Figure 1This is a graph showing the relationship between the photosynthetic rate and photosynthetic photon flux density (PPFD) of the asparagus variety JX1902 of this invention.

[0033] Figure 2 This is a graph showing the relationship between photosynthetic rate and time for asparagus varieties JX1902 and JX2332 of this invention. Detailed Implementation

[0034] The following embodiments are provided to better understand the present invention and are not limited to the preferred embodiments described. They do not constitute a limitation on the content and scope of protection of the present invention. Any product that is the same as or similar to the present invention, derived by any person under the guidance of the present invention or by combining the features of the present invention with other prior art, falls within the protection scope of the present invention.

[0035] For experiments not specifically described in the examples, the procedures or conditions should be followed according to the conventional experimental procedures described in the literature in this field. Reagents or instruments whose manufacturers are not specified are all commercially available conventional reagent products.

[0036] In the preliminary study, the pseudo-leaf (PPFD) of the upper part of the asparagus plants (10 cm from the top of the plant) of six asparagus varieties (JX1902, JX2245, JX2112, JX2188, JX2332, and JH111) during the juvenile stage was measured using a quantum sensor Li 6400 and the photosynthetic rate was measured using a portable photosynthesis meter (Li 6400). Linear fitting was then performed. The linear fitting results for asparagus variety JX1902 are shown below. Figure 1 As shown, the results revealed a direct correlation between photosynthetic rate and PPFD. Simultaneously, the photosynthetic rate of the pseudo-leaf (10 cm from the top of the plant) at the upper part of the young asparagus plants of the above six asparagus varieties was measured at different times of the day, and a graph was plotted with the measurement time as the x-axis and the photosynthetic rate as the y-axis. Results for asparagus varieties JX1902 (high yield, 2200.13 catties / mu) and JX2332 (low yield, 1905.53 catties / mu) are shown below. Figure 2 As shown, the photosynthetic rates of high-yielding asparagus and low-yielding asparagus differ at different times of day. The differences are more significant when the measurement time range is 10-16 am, and preferably, the differences are most significant when the measurement time range is 12-14 am.

[0037] In previous studies, a comparative study of sucrose synthase and acid invertase activities was conducted on young asparagus plants of varieties JX1902 and JX2332. Plants with tender stems of 30 cm in length were selected. For each plant, five plants were selected, and the activities of acid invertase (D) and sucrose synthase (F) were measured within the tender stem area from the top of the plant to 10 cm from the top. For another five plants, five more plants were selected, and the activities of acid invertase (D) and sucrose synthase (F) were measured within the tender stem area from 10-30 cm from the top of the plant. The results are shown in Table 1 below. Significant differences in acid invertase activity were observed among different varieties when measuring the tender stem area from the top of the young asparagus plants (10-30 cm from the top).

[0038] Table 1. Detection values ​​of different enzymes

[0039]

[0040] Note: In a and b, the same letter indicates no significant difference, while different letters indicate a significant difference.

[0041] Example 1

[0042] This embodiment provides a method for evaluating asparagus yield, including the following steps:

[0043] S1. Cultivation of Asparagus in the Young Plant Stage: Six asparagus varieties with known yields were selected: JX1902, JX2245, JX2112, JX2188, JX2332, and JH111, with JH111 serving as a control. The seeds of the asparagus varieties were disinfected by soaking in a 3% sodium hypochlorite solution for 8 minutes, then rinsed with sterile water and soaked in sterile water for 35 hours. The seeds were then placed in petri dishes and germinated at 27℃ for 3 days. After germination, the seeds with uniform white sprouts were sown in nutrient soil (peat moss:sand = 3:1). After 45 days of cultivation, they were transplanted into a greenhouse, with 25 plants of each variety transplanted at a density of 20cm × 20cm. Cultivation continued for one year until the asparagus entered the young plant stage and all the pseudo-leafs had fully unfolded.

[0044] S2. Measurement of photosynthetic rate A: The photosynthetic rate A was measured using a portable photosynthesis meter (Li 6400) on the four sides of the upper part of each young asparagus plant during the time range of 12-13 o'clock in the day.

[0045] Measurement of S3 and ribulose-1,5-bisphosphate carboxylase / oxygenase activity B: Nine plants were randomly selected from each variety, with three plants as a group. The pseudo-leafs on the four sides of the upper part of each young asparagus plant were taken, mixed and 0.5g was taken and frozen in liquid nitrogen. The Rubisco enzyme activity was determined using the Rubisco enzyme activity kit.

[0046] S4. Measurement of leaf dry weight C per unit area: Randomly select 5 plants from each variety, and take all the pseudo-leafs from the upper part of each young asparagus plant. Place them in a forced-air drying oven and dry them at a temperature of 70℃ until the moisture content is 0%. Then measure the area and dry weight of the pseudo-leafs after drying, and calculate C using the following formula: Formula: C = Dry weight (g) / Area (cm²) 2 ;

[0047] S5. Measurement of acid invertase activity D: Five plants with a tender stem length of 30cm from the young asparagus plants of each variety were selected. The tender stems within a range of 12.5-17.5cm from the top of the plant were taken, and the acid invertase activity was measured using an acid invertase kit.

[0048] S6. Measurement of root-to-shoot ratio E: Randomly select 5 plants from each variety, cut each plant off from the base, dig out the asparagus root system (the underground part of the asparagus), wash the asparagus root system with clean water, and then dry the underground part and the above-ground part until the moisture content is 0%. Measure the weight of the underground part and the above-ground part, and calculate the root-to-shoot ratio according to the following formula: E = weight of underground part / weight of above-ground part.

[0049] S7. Evaluation Value Calculation: The measured values ​​of indicators A, B, C, D, and E are used to calculate the evaluation value using the following formula. The asparagus yield is then evaluated based on the evaluation value. The formula is: in, The sum of A values ​​for asparagus from the first variety to the i-th variety during the juvenile stage. The sum of the B values ​​for asparagus from the first variety to the i-th variety during the juvenile stage. The sum of C values ​​for asparagus from the first variety to the i-th variety during the juvenile stage. The sum of the D values ​​of asparagus seedlings from the first variety to the i-th variety during their juvenile stage. A is the sum of the E values ​​of asparagus from the first variety to the i-th variety during the juvenile stage; i Let A be the value of asparagus in the juvenile stage of the i-th variety, and B be the value of B. i Let B be the value of the i-th variety of asparagus in its juvenile stage, and C be the value of the juvenile asparagus. i Let C be the value of the i-th variety of asparagus in its juvenile stage, and D be the value of the juvenile asparagus. i Let D be the value of the i-th variety of asparagus in its juvenile stage, and E be the value of the juvenile asparagus. i Let E be the value of the juvenile asparagus of the i-th variety.

[0050] The yield data for six asparagus varieties, JX1902, JX2245, JX2112, JX2188, JX2332, and JH111, are derived from the actual annual harvest yield measurement results.

[0051] The results are shown in Table 2 below. Six asparagus varieties with known yields were selected for plant cultivation, and the following indicators were measured: photosynthetic capacity (photosynthetic rate A, RUBISCO enzyme activity B), mass transport (leaf dry weight per unit area C, acid invertase activity D), and sink storage capacity (root-to-shoot ratio E). Evaluation values ​​were then calculated using formulas, and the significance level was calculated using SPSS software. Table 2 shows that, using asparagus variety JH111 as a control, the evaluation values ​​of asparagus varieties JX1902 and JX2245 were higher than those of asparagus variety JH111. Using the evaluation method of this invention, it can be determined that the yields of asparagus varieties JX1902 and JX2245 are greater than that of asparagus variety JH111. Comparing the evaluation results with the actual asparagus yield confirms the accuracy of the evaluation results, and a positive correlation between the evaluation value and yield can be established. The evaluation values ​​and yield data of asparagus varieties JX2112, JX2188, and JX2332 also indicate that the evaluation method of this invention can be used to evaluate asparagus yield.

[0052] Using JH111 as a control, varieties with evaluation values ​​close to or higher than those of JH111 were selected for subsequent breeding; that is, the evaluation value of the tested variety was greater than the evaluation value of the control by 1. However, evaluating asparagus yield using only one indicator, such as the photosynthetic rate ratio between varieties JX1902 and JH111, or the root-to-shoot ratio between varieties JH111 and JX2332, cannot provide an accurate and reasonable assessment.

[0053] This embodiment can evaluate asparagus yield through assessment values ​​in less than 1.5 years, which greatly shortens the evaluation time compared with the prior art.

[0054] Table 2. Evaluation and comparison results of yields of 6 known varieties selected in Example 1

[0055]

[0056] Note: In a, b, c, and d, the same letter indicates no significant difference, while different letters indicate a significant difference.

[0057] Comparative Example 1

[0058] This comparative example provides a method for evaluating asparagus yield. The evaluation method is basically the same as that in Example 1, except that step S5 is different. In this comparative example, step S5 is the measurement of sucrose synthase activity F: Five plants with a tender stem length of 30 cm from the young asparagus plants of each variety are selected, and tender stems within a range of 12.5-17.5 cm from the top of the plant are taken. The activity of sucrose synthase is measured using a sucrose synthase activity kit.

[0059] The results of the determination of sucrose synthase activity (F) and ratio in Comparative Example 1, and acid invertase activity (D) and ratio in Example 1 are shown in Table 3 below. Combined with Table 2, Table 3 shows that the activity of sucrose synthase does not correlate with yield trends. For example, compared to asparagus varieties JX2112, asparagus variety JH111 has lower sucrose synthase activity but higher yield, while asparagus variety JX2112 has higher sucrose synthase activity but lower yield. Secondly, there is no significant difference in the ratio of sucrose synthase activity among different varieties. If sucrose synthase activity is used as one of the evaluation indicators, asparagus yield cannot be accurately evaluated. The activity of acid invertase in different asparagus varieties shows a consistent trend with yield, and the ratio of acid invertase activity differs significantly. Combining the evaluation results and yield data in Table 2, using acid invertase activity as an evaluation indicator can accurately evaluate asparagus yield.

[0060] Table 3. Detection values ​​of different enzymes

[0061]

[0062]

[0063] Note: In a, b, c, bc, the same letter indicates no significant difference, and different letters indicate a significant difference.

[0064] The activity F of sucrose synthase was used as an evaluation index to calculate the evaluation value. It was found that the evaluation value obtained by using the activity F of sucrose synthase as an evaluation index did not match the actual yield trend, which verified that the activity of sucrose synthase cannot be used as an evaluation index. The results are shown in Table 4.

[0065] Table 4 shows the evaluation and comparison results of the yields of six known varieties.

[0066]

[0067] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A method for evaluating asparagus yield, characterized in that, The steps include the following: S1. Obtain young asparagus plants; S2. Measure the following parameters of young asparagus plants of i varieties: photosynthetic rate A, ribulose-1,5-bisphosphate carboxylase / oxygenase activity B, leaf dry weight per unit area C, acid invertase activity D, and root-to-shoot ratio E; the acid invertase activity D was measured from the tender stems of young asparagus plants 10-30 cm from the top. S3. Calculate the evaluation value according to the following formula, and evaluate the asparagus yield based on the evaluation value. , i≥2, where, The sum of A values ​​for asparagus from the first variety to the i-th variety during the juvenile stage. The sum of the B values ​​for asparagus from the first variety to the i-th variety during the juvenile stage. The sum of C values ​​for asparagus from the first variety to the i-th variety during the juvenile stage. The sum of the D values ​​of asparagus seedlings from the first variety to the i-th variety during their juvenile stage. The sum of E values ​​for asparagus from the first variety to the i-th variety during the juvenile stage; A i Let A be the value of asparagus in the juvenile stage of the i-th variety, and B be the value of B. i Let B be the value of the i-th variety of asparagus in its juvenile stage, and C be the value of the juvenile asparagus. i Let C be the value of the i-th variety of asparagus in its juvenile stage, and D be the value of the juvenile asparagus. i Let D be the value of the i-th variety of asparagus in its juvenile stage, and E be the value of the juvenile asparagus. i Let E be the value of the juvenile asparagus of the i-th variety; In step S1, the asparagus seeds to be tested are germinated, sown, transplanted, and cultured to obtain the young asparagus plants. The germination process includes soaking the asparagus seeds to be tested in water for 30-36 hours, and then placing the seeds in an environment with a temperature of 25-28℃ for 2-3 days to germinate; the process also includes disinfecting the asparagus seeds to be tested with disinfectant before soaking. The sowing process involves sowing the germinated seeds (which have sprouted white seeds) in nutrient soil and cultivating them for 43-48 days. After cultivation, the seeds are transplanted into a greenhouse and then cultivated for another 12-13 months. The photosynthetic rate A was measured from the pseudo-leaf of the upper part of the young asparagus plant. The measurement time range for the photosynthetic rate A is 12-14 o'clock; The sample used to measure the activity B of ribulose-1,5-bisphosphate carboxylase / oxygenase was the pseudo-leaf of the upper part of the young asparagus plant. The sample for measuring the leaf dry weight C per unit area was the pseudo-leaf of the upper part of the young asparagus plant after drying treatment. The root-to-shoot ratio E is the dry matter weight ratio of the underground and above-ground parts of asparagus during the young plant stage.

2. The method for evaluating asparagus yield according to claim 1, characterized in that, The activity D of the acid invertase was measured in young asparagus stems 12.5-17.5 cm from the top.

Images