A novel method for preparing medical collagen sponges

Abstract

This invention provides a novel method for preparing a medical collagen sponge, relating to the field of medical sponge technology. The invention uses yeast extract obtained by Soxhlet extraction of brewer's yeast as a raw material, which is mixed and reacted with polyvinyl alcohol and graphene oxide to obtain the novel medical collagen sponge. The medical collagen sponge prepared by this invention exhibits excellent performance and good hemostatic effect. Furthermore, the preparation method is simple, and it degrades postoperatively, demonstrating significant application value in the field of medical materials.

Description

Technical Field

[0001] This invention relates to the field of medical sponge technology, and in particular to a method for preparing a novel medical collagen sponge. Background Technology

[0002] Medical sponges are primarily used in wound hemostasis to accelerate wound healing. Collagen hemostatic sponges are widely used clinically.

[0003] Among the existing methods for preparing collagen medical sponges, some require the addition of cross-linking agents, but most of the remaining cross-linking agents are toxic reagents that pose safety risks to the human body; others are extracted from natural products, such as fish skin and pig trotters. Although these reagents are non-toxic, the materials are prone to deformation or even breakage when exposed to water, and the hemostatic effect cannot be guaranteed. Summary of the Invention

[0004] The purpose of this invention is to provide a novel method for preparing medical collagen sponges to solve the problems existing in the prior art.

[0005] To achieve the above objectives, the present invention provides the following solution:

[0006] One of the technical solutions of this invention is to provide a medical collagen sponge, comprising the following raw material components in parts by weight:

[0007] 5-30 parts yeast extract and 2-50 parts polyvinyl alcohol;

[0008] The yeast extract is obtained by Soxhlet extraction of brewer's yeast at 120°C under alkaline conditions.

[0009] The main components of this yeast extract are proteins and carbohydrates.

[0010] As a further preferred embodiment of the present invention, the Soxhlet extraction time is 24 hours.

[0011] The second technical solution of this invention provides another type of medical collagen sponge, comprising the following raw material components in parts by weight:

[0012] 5-30 parts yeast extract, 2-50 parts polyvinyl alcohol, and 5-50 parts graphene oxide;

[0013] The yeast extract is obtained by Soxhlet extraction of brewer's yeast at 120°C under alkaline conditions.

[0014] As a further preferred embodiment of the present invention, the Soxhlet extraction time is 24 hours.

[0015] The third technical solution of this invention provides a method for preparing the above-mentioned medical collagen sponge, comprising the following steps:

[0016] The yeast extract and polyvinyl alcohol were mixed in water, and the resulting aqueous solution was left at room temperature for 1-24 hours. After that, it was freeze-dried to obtain the medical collagen sponge.

[0017] As a further preferred embodiment of the present invention, the freeze-drying process further includes a vacuum sterilization step.

[0018] The fourth technical solution of the present invention provides another method for preparing the above-mentioned medical collagen sponge, comprising the following steps:

[0019] The yeast extract, polyvinyl alcohol, and graphene oxide were mixed in water, and the resulting aqueous solution was left at room temperature for 1-24 hours. After that, it was freeze-dried to obtain the medical collagen sponge.

[0020] As a further preferred embodiment of the present invention, the freeze-drying process further includes a vacuum sterilization step.

[0021] The fifth technical solution of this invention is to provide the application of the above-mentioned medical collagen sponge as a hemostatic material.

[0022] The medical sponge preparation method of the present invention is simple, green and environmentally friendly. It does not require a freezing and thawing process and can prepare a medical sponge with good performance at room temperature.

[0023] The reagents used in this invention are all derived from natural extracts or natural polymers. No toxic additives or cross-linking agents are required during the preparation process. The resulting medical sponge has good performance, is not easily broken, and has a good hemostatic effect.

[0024] The present invention discloses the following technical effects:

[0025] This invention provides a medical collagen sponge with excellent material properties and good hemostatic effect.

[0026] The method for preparing the medical collagen sponge of the present invention is simple, can be degraded after surgery, and has important application value in the field of medical materials. Attached Figure Description

[0027] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0028] Figure 1The images show the medical sponge #2 prepared in Example 2 of this invention before and after soaking in pig blood; (a) before soaking, (b) after soaking.

[0029] Figure 2 The images show the medical sponge #4 prepared in Example 4 of this invention before and after soaking in pig blood; (a) before soaking, and (b) after soaking. Detailed Implementation

[0030] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0031] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0032] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0033] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be readily apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0034] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0035] The brewer's yeast powder used in the embodiments of the present invention was purchased from Aladdin Biochemical Technology Co., Ltd., CAS No. 119-44-8; the fish collagen peptide powder used in the comparative example was purchased from Aladdin Biochemical Technology Co., Ltd., CAS No. 9064-67-9, with a protein purity of 90%.

[0036] Example 1

[0037] The mass proportions of raw materials used in the preparation of medical sponges are as follows:

[0038] 30 parts yeast extract and 5 parts polyvinyl alcohol.

[0039] Preparation method of medical sponge:

[0040] (1) Brewer's yeast powder was extracted in an alkaline sodium hydroxide solution with pH=11 at 120℃ for 24 hours to obtain yeast extract.

[0041] (2) The yeast extract obtained in step (1) was mixed with polyvinyl alcohol in an aqueous solution (total concentration after mixing was 8 mg / mL), stirred at low speed until homogeneous, and placed at room temperature for 12 hours. The sample was then freeze-dried at -50℃ for 24 hours, and finally sterilized by irradiation at a wavelength of 280 nm for 30 min. The resulting product was labeled as Medical Sponge No. 1.

[0042] Example 2

[0043] The mass proportions of raw materials used in the preparation of medical sponges are as follows:

[0044] 30 parts yeast extract, 5 parts polyvinyl alcohol, and 10 parts graphene oxide.

[0045] Preparation method of medical sponge:

[0046] (1) Brewer's yeast powder was extracted in an alkaline sodium hydroxide solution with pH=11 at 120℃ for 24 hours to obtain yeast extract.

[0047] (2) The yeast extract, polyvinyl alcohol, and graphene oxide prepared in step (1) were mixed in an aqueous solution (total concentration after mixing was 8 mg / mL), stirred at low speed until homogeneous, and placed at room temperature for 12 hours. The sample was then freeze-dried at -50℃ for 24 hours, and finally sterilized by irradiation at a wavelength of 280 nm for 30 minutes. The resulting product was labeled as Medical Sponge No. 2.

[0048] Example 3

[0049] The mass proportions of raw materials used in the preparation of medical sponges are as follows:

[0050] 30 parts yeast extract and 5 parts polyvinyl alcohol.

[0051] (1) Brewer's yeast powder was extracted in an alkaline sodium hydroxide solution with pH=11 at 120℃ for 24h to obtain yeast extract.

[0052] (2) The yeast extract obtained in step (1) was mixed with polyvinyl alcohol in an aqueous solution (total concentration after mixing was 8 mg / mL), stirred at low speed until homogeneous, placed at room temperature for 12 hours, then frozen at -25℃ for 12 hours, and then freeze-dried at -50℃ for 24 hours. Finally, the sample was sterilized by irradiation at a wavelength of 280 nm for 30 minutes. It was labeled as medical sponge #3.

[0053] Example 4

[0054] The raw materials used in the preparation of medical sponges are as follows (by weight):

[0055] 30 parts of yeast extract.

[0056] Preparation method:

[0057] (1) Brewer's yeast powder was extracted in an alkaline sodium hydroxide solution with pH=11 at 120℃ for 24h to obtain yeast extract.

[0058] (2) The yeast extract prepared in step (1) was placed at room temperature for 12 hours, then frozen at -25℃ for 12 hours, and then freeze-dried at -50℃ for 24 hours. Finally, it was sterilized by irradiation at a wavelength of 280nm for 30 minutes. It was labeled as medical sponge #4.

[0059] Comparative Example 1

[0060] The raw materials used in the preparation of medical sponges are as follows (by weight):

[0061] 30 parts fish collagen peptide powder and 5 parts polyvinyl alcohol;

[0062] Preparation method:

[0063] Fish collagen peptide powder and polyvinyl alcohol were mixed in an aqueous solution (total concentration after mixing was 8 mg / mL), stirred at low speed until homogeneous, and left at room temperature for 12 hours. The sample was a gel-like fluid, and no gel solid sample was formed.

[0064] The medical sponge sample 2# prepared according to the present invention was immersed in 10 mL of pig blood. After 12 minutes, medical sponge 2# completely absorbed the pig blood, and the sponge remained intact without breakage or bleeding. A small amount of pig blood was released after squeezing. Under the same conditions, medical sponge 1# absorbed the blood more slowly, taking 20 minutes to absorb it completely. Medical sponges 3# and 4#, being more brittle, broke after absorbing the pig blood.

[0065] Figure 1 The images show the medical sponge #2 prepared in Example 2 of this invention before and after soaking in pig blood; (a) before soaking, (b) after soaking. Figure 2 The images show the medical sponge #4 prepared in Example 4 of this invention before and after soaking in pig blood; (a) before soaking, and (b) after soaking.

[0066] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A medical sponge, characterized in that, The raw material components include the following parts by weight: 5-30 parts yeast extract, 2-50 parts polyvinyl alcohol, and 5-50 parts graphene oxide; Brewer's yeast powder was extracted in an alkaline sodium hydroxide solution with pH=11 at 120℃ for 24 hours to obtain yeast extract; The main components of this yeast extract are proteins and carbohydrates; The preparation method of the medical sponge includes the following steps: mixing the yeast extract, polyvinyl alcohol and graphene oxide in water, placing the resulting mixed aqueous solution at room temperature for 1-24 hours, and then performing freeze-drying and vacuum sterilization to obtain the medical sponge.

2. The application of the medical sponge as described in claim 1 as a hemostatic material.

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