A spherical segmented feeding intestinal bionic reactor and its control method

Abstract

The present invention belongs to the technical field of bioreactors, and is a spherical segmented feeding intestinal bionic reactor and a control method thereof. The bionic reactor comprises a tank body, a bionic intestinal device, a spherical material container, a feeding device, a discharging device and a stirring device; the feeding device is arranged at the upper end of the tank body, the discharging device is arranged at the lower end of the tank body, the bionic intestinal device and the stirring device are arranged inside the tank body, one end of the bionic intestinal device is connected to the feeding device, and the other end is connected to the discharging device; the present invention designs the specific connection and execution mode of the intestine and the reactor, so that quantitative addition of materials becomes possible, the discharged materials can be conveniently collected and detected, and the scum problem of cellulose materials can be reduced; the intestinal structure cooperates with the spherical material container, which can meet the precise hydraulic retention time of the material and optimize the biochemical reaction efficiency.

Description

Technical Field

[0001] The invention belongs to the technical field of bioreactors, and in particular relates to a spherical segmented feeding intestinal bionic reactor and a control method thereof. Background Art

[0002] With the increasing attention paid to carbon emissions and carbon reduction around the world, developing biomass energy and recycling it has become an important task. Agricultural waste is considered to be a high-quality renewable biological resource because it is rich in organic matter such as nitrogen, phosphorus, and potassium. Using microorganisms to perform anaerobic fermentation to produce methane and hydrogen, and using the fermentation residues for organic fertilizers, greenhouse planting, etc., is a treatment method that helps the green, low-carbon, and high-quality development of agriculture.

[0003] At present, when agricultural waste is used as raw material for anaerobic fermentation, solid and liquid are mixed and fermented together in the tank. The gas produced by microbial fermentation causes the cellulose materials to float and form a crust, so that the newly added materials and the original materials in the tank cannot fully contact the microorganisms for reaction, resulting in insufficient fermentation and low gas production efficiency. In addition, as the materials gradually accumulate, the discharge port may also be blocked, which will affect the normal reaction.

[0004] Therefore, it is urgent to design an efficient and reliable bioreactor to address the problems of fermentation accumulation and blockage of materials, failure to separate solids and liquids, and poor data representativeness caused by mismatch between input and output materials. Summary of the invention

[0005] The technical problem to be solved by the present invention is that the existing bioreactors have defects such as sediment accumulation, uneven mixing, and inability to screen and fix materials. In order to overcome the deficiencies and defects mentioned in the above background technology, a spherical segmented feeding intestinal bionic reactor and a control method thereof are provided.

[0006] In order to solve the above technical problems, the technical solution proposed by the present invention is:

[0007] A spherical segmented feeding intestinal bionic reactor, the bionic reactor comprising a tank body, a bionic intestinal device, a feeding device, a discharging device and a stirring device; the feeding device is arranged at the upper end of the tank body, the discharging device is arranged at the lower end of the tank body, the bionic intestinal device and the stirring device are arranged inside the tank body, one end of the bionic intestinal device is connected to the feeding device, and the other end is connected to the discharging device;

[0008] The bionic intestinal device comprises one or more bionic intestinals, the bionic intestinals are in the form of a hollow tube structure, and a stirring device is arranged in a spiral around the inside of the tank;

[0009] The spherical material container is a hollow spherical structure, whose diameter is smaller than the inner diameter of the bionic intestinal device and cannot escape from the hollow part of the hollow tube structure; the material is placed inside the spherical material container and is put into the bionic reactor through the spherical material container along the feeding device.

[0010] The present application designs a spherical segmented feeding intestine bionic reactor. After the material is put into the feed port, it falls in the bionic intestine and reacts with the microorganisms in the reactor. During the reaction, the material is stabilized in the spherical material container and is not easy to disperse. It can also avoid accumulation and precipitation from contact with the inner wall of the reactor or the agitator, thereby ensuring a good solid-phase separation effect. The material spirally falls through the intestinal structure and is stirred with a stirring device, which is beneficial to increasing the reaction time and contact area between the material and the microorganisms in the inoculated mud, thereby increasing the reaction rate. The generated biogas passes through the outer wall of the bionic intestine and enters the tank, and overflows through buoyancy, and then realizes gas phase separation, thereby greatly improving the reaction rate and continuous reaction efficiency.

[0011] Furthermore, the present application designs a spherical material container that matches the bionic intestine, and the device has the following advantages:

[0012] (1) Carrying materials so that they can fall smoothly in the bionic intestine and can achieve the effect of adding materials in sections;

[0013] When using a traditional reactor, there will be problems such as the mixing and accumulation of materials reacted the previous day and the materials reacted the next day, and the fermentation capacity will decrease due to the unevenness of the materials and the fermentation liquid, which will affect the accuracy of the experimental results. The present application adopts a spherical material container to add materials, and the materials added at different time periods are fixed inside the spherical device, which will not be mixed during the reaction process, and the reaction process can be well controlled to ensure that the material reaction time can be accurately matched during the material collection stage.

[0014] By loading cellulose raw materials such as straw or energy crops into a spherical material container and placing it in a bionic intestinal device, solid-liquid-gas separation is achieved; the material reacts with the microorganisms in the inoculated mud through the spherical material container and the bionic intestinal structure, and the generated gas escapes from the hollow position, which can avoid the problem of material agglomeration or floating in a certain section of the bionic intestine during the reaction; (3) The bionic intestinal structure is coordinated with the design of the spherical material container. The bionic intestinal structure fixes the running path of the spherical material container, which can extend the sliding time of the spherical material container in the device and avoid the problem of incomplete reaction.

[0015] Preferably, the tank body comprises a reactor tank body, and the inoculated mud is stored in the reactor tank body.

[0016] Under the action of mechanical stirring, the inoculum mud in the tank body flows, and it will not be unable to stir or block the bionic intestinal device due to the excessive viscosity of the inoculum mud during the reaction process. It is beneficial to disperse the VFA and free ammonia nitrogen accumulated in the inoculum mud, and improve the microbial activity and degradation efficiency. These advantages work together to effectively ensure the stability of the fermentation process of the bionic intestinal reactor. On the other hand, stirring can accelerate the escape of the generated biogas and realize the separation of the gas and solid-liquid mixed area.

[0017] Preferably, the bionic intestine is in a spiral shape, which includes a starting section, a spiral section and an exit section;

[0018] The starting section is connected to the feeding device, and the angle between the starting section and the horizontal plane is 40-90°;

[0019] The spiral section is arranged along the inner wall of the tank, and the angle between the spiral section and the horizontal plane at any point in the falling direction is 30-60°;

[0020] The outlet section is connected to the discharging device, and the angle between the outlet section and the horizontal plane is 40-90°.

[0021] The spiral section is arranged close to the inner wall of the tank and can be wound in one section, one circle or multiple circles to meet the reaction time required for the degradation of different materials according to the requirements of the height-to-diameter ratio and residence time of different bioreactors.

[0022] The spherical material container enters the spiral section along the starting section. Due to the design of the spiral section, the spherical material container can fall at a certain speed to avoid blockage and jamming; it can also fall slowly to control the reaction time, which is more conducive to the full reaction of the material. In combination with the stirring device, the mixing degree of the material in the intestine and the liquid in the tank is further improved, the mass transfer and stirring effects are improved, and the gas production is increased. At the same time, due to the hollow structure of the bionic intestine, the generated gas is discharged from the hollow position to avoid the phenomenon of pushing the material up.

[0023] Preferably, the material of the bionic intestine is at least one of metal or plastic, and the inner diameter of the bionic intestine is 1 / 10-1 / 4 of the inner diameter of the bionic reactor.

[0024] More preferably, the bionic intestine adopts a hollow design as a whole, which can increase the mixing surface area of ​​the microbial mud and the material. When a metal or plastic hollow material is used, its porosity is between 50% and 95%.

[0025] Preferably, the spherical material container comprises a hemispherical shell, a fastening knob, and a buckle, and the two hemispherical shells are connected by the fastening knob and then opened by the buckle.

[0026] More preferably, the ratio of the total height of the spiral segment in the vertical direction to the radius of the circular projection formed by the spiral segment in the horizontal direction is 2-3, and the ratio of the total height of the spiral segment in the vertical direction to the height of the tank body is 18%-42%.

[0027] The material of the spherical material container is at least one of metal and plastic; the diameter of the spherical material container is 2-5 mm smaller than the inner diameter of the bionic intestinal tube.

[0028] The material in the spherical material container can fully contact with the inoculated mud, while ensuring a good solid phase separation effect. The generated biogas passes through the hollow part and the outer wall of the bionic intestine and then enters the tank and overflows, realizing the entire reaction process. The size of the spherical material container is smaller than the diameter of the bionic intestine, ensuring that the spherical material container falls smoothly in the intestine.

[0029] Preferably, the feeding device comprises one or more feed ports, each of which comprises a feed conduit, which is funnel-shaped and has an open funnel end and a sealed end, wherein the sealed end is connected to below the liquid level of the inoculated mud in the reactor tank and is connected to the bionic intestine, the opening diameter of the open funnel end is larger than the spherical diameter of the spherical material container, and the open funnel end is sealed with a metal clamp and a rubber gasket.

[0030] The design of the feeding device ensures the effective sealing of the feeding port and the position below the liquid level to prevent biogas leakage. At the same time, it allows multiple feeding ports to be set according to the actual reaction tank volume and fermentation requirements. The feeding port is sealed with a metal clamp and a rubber gasket, which is opened during feeding and sealed and locked under normal conditions to prevent biogas leakage. The opening diameter of the open funnel end is larger than the spherical diameter of the spherical material container, which facilitates the addition and entry of the spherical material container.

[0031] Preferably, the stirring device includes a motor, a coupling and a stirring shaft. The motor is arranged on the outside of the reactor tank body and is connected to the stirring shaft through the coupling. The stirring shaft vertically passes through the outer shell of the reactor tank body to the inside of the tank body. The stirring shaft extends below the liquid level of the inoculum mud in the reactor tank body. One or more stirring blades are arranged on the stirring shaft.

[0032] The motor drives the stirring shaft to rotate, which accelerates the mixing of the metal hollow spherical material container and the surrounding inoculum mud, improves the heat and mass transfer rates, maintains the stability of the microbial community structure, and promotes the reaction.

[0033] Preferably, the surface of the paddle blade is fan-shaped and is connected to the stirring shaft through fastening sleeve one and fastening sleeve two. Fastening sleeve one controls the angle between the paddle blade and the horizontal plane, and fastening sleeve two controls the blade height of the paddle blade and the direction of the blade in the horizontal direction of the stirring shaft.

[0034] The direction, number and height of the blades can be adjusted on the stirring shaft, which can adapt to intestinal structures of different lengths and angles, evenly disperse the locally accumulated ammonia nitrogen and VFA, achieve better stirring effects, maintain the stability of the microbial community structure in the reactor, and avoid acid inhibition.

[0035] More preferably, the stirring blades include but are not limited to single propeller blades and / or double propeller blades.

[0036] Preferably, the feeding device comprises one or more feeding ports, the feeding port is in the shape of a funnel, having an open funnel end and a sealed end, wherein the sealed end is connected to below the liquid level in the reactor tank through a feeding conduit, and is connected to the bionic intestine through a clamp, and the open funnel end is sealed;

[0037] The design of the feeding device ensures the effective sealing of the feeding port and the position below the liquid level to prevent biogas leakage, while allowing multiple feeding ports to be set according to the actual reaction tank volume and fermentation requirements; the feeding port is sealed with a metal clamp and a rubber gasket, which is opened during feeding and sealed and locked under normal circumstances to prevent biogas leakage.

[0038] Preferably, the discharging device includes a primary discharging mechanism and a secondary discharging mechanism, the primary discharging mechanism and the secondary discharging mechanism are connected through a curved pipe; the primary discharging mechanism is connected to the bionic intestine, and a pull-out baffle is designed between the primary discharging mechanism and the bionic intestine;

[0039] Preferably, the discharging device comprises a discharging port and a water stop valve.

[0040] Preferably, the bionic reactor further comprises a temperature control system, and the temperature control system comprises a water bath jacket arranged outside the reactor tank;

[0041] The water bath jacket is heated or cooled by a hot water circulation system, so that the fermentation process is carried out within the optimal temperature range, ensuring the activity of the microorganisms and the fermentation efficiency. The temperature control of the water bath jacket is precise and can be adjusted according to the fermentation needs, thereby improving the overall performance of the system.

[0042] The reactor tank is also provided with a glass window and a support base. The glass window is arranged corresponding to the liquid level of the inoculated mud, so as to facilitate observation of the distribution of the fermentation liquid level and the gas production.

[0043] Under the same technical concept, the present application also provides a control method for a spherical segmented feeding intestinal bionic reactor, comprising the following steps:

[0044] (1) Adding inoculum mud into the tank of the spherical segmented feeding intestine biomimetic reactor;

[0045] (2) placing the material into a spherical material container and feeding it into the bionic intestinal device through a feeding device, and starting a stirring device to stir the material to cultivate the inoculated mud;

[0046] (3) After the inoculation mud is cultivated, it is put into the spherical material container. After the reaction between the material and the inoculation mud is completed, the discharging device is opened to discharge the spherical material container and collect the material therein.

[0047] More preferably, the inoculated mud should be sieved to remove impurities larger than 10 mm before use, and the mass ratio of VS in the inoculated mud is 0.7%-3%;

[0048] Preferably, before adding the material in step (3), an organic load is set, and the material is quantitatively added according to the organic load; and in step (3), when discharging the spherical material container, only the spherical material container is discharged, and the seeding mud is retained in the bionic reactor for repeated use.

[0049] More preferably, the material is a substrate including cellulose material, high-solid livestock and poultry manure, and energy crops.

[0050] Compared with the prior art, the present invention has the following beneficial effects:

[0051] (1) The present invention is based on the traditional CSTR fully mixed anaerobic reactor and process, introduces intestinal bionic technology, and designs the specific connection and execution mode between the intestine and the reactor. The two-phase separation reaction of the material and the inoculated mud is realized through the intestinal bionic system, which has made new improvements and breakthroughs in the structure and operation mode of the bioreactor;

[0052] (2) The spherical material container is designed in conjunction with the bionic intestine to facilitate the feeding and discharging of the reactor. The discharged materials can be easily collected and tested, and the reaction interference caused by the materials floating on the upper part of the reactor can be reduced. The intestinal structure is combined with the spherical material container to meet the precise hydraulic retention time of the materials and control the reaction process.

[0053] (3) During the actual operation, the bionic intestinal device of the present invention can ensure that the solid material and the inoculated mud are fully in contact with each other while being separated and added, thereby improving the mass transfer effect and being able to discharge the reaction gas in time; the present invention effectively improves the anaerobic fermentation efficiency and methane production, and provides an efficient and stable microbial anaerobic fermentation solution;

[0054] (4) By changing the intestinal layout, the degradation of different materials can be achieved. By changing the layout position of the paddles, the locally accumulated ammonia nitrogen and VFA can be evenly dispersed, so that the stirring effect can be better, the stability of the microbial community structure in the reactor can be maintained, and acid inhibition can be avoided. BRIEF DESCRIPTION OF THE DRAWINGS

[0055] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0056] Figure 1 1 is a schematic diagram of the internal cross-sectional structure of the spherical segmented feeding intestinal biomimetic reactor of Example 1;

[0057] Figure 2 1 is a schematic diagram of the external structure of the spherical segmented feeding intestinal biomimetic reactor of Example 1;

[0058] Figure 3 This is a schematic diagram of the structure of a spherical material container of a spherical segmented feeding intestinal biomimetic reactor in Example 1;

[0059] Figure 4 This is a schematic diagram of the bionic intestinal structure of the spherical segmented feeding intestinal bionic reactor of Example 2;

[0060] Figure 5 This is a schematic diagram of the microbial reactor structure of Comparative Example 1;

[0061] Figure 6 This is a schematic diagram of the microbial reactor structure of Comparative Example 2;

[0062] Figure 7 is a comparison chart of gas production efficiency of microbial fermentation reaction in Example 1 and Comparative Example 1;

[0063] Figure 8 It is a comparison chart of gas production efficiency of microbial fermentation reaction in Example 1 and Comparative Example 2;

[0064] Fig. 9 Schematic diagram of the stirring shaft structure of Example 1;

[0065] Fig.10 This is a schematic diagram of the bionic intestinal structure.

[0066] Among them, 1. motor; 2. feed port; 3. bionic intestine; 4. spherical material container; 5. water bath jacket; 51. reactor tank; 6. primary discharge mechanism; 7. secondary discharge mechanism; 8. coupling; 9. glass window; 10. stirring shaft; 11. support base; 12. connecting elbow; 13. feed duct; 14. hemispherical shell; 15. buckle; 16. tightening knob; 21. clamp; 22. exhaust port; 23. first sampling port; 24. second sampling port; 25. third sampling port; 26. water inlet; 27. water outlet; 28. pull-out baffle; 29. ​​paddle; 30. tightening sleeve one; 31. tightening sleeve two; A. feed section; B. spiral section; C: outlet section. DETAILED DESCRIPTION

[0067] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively and meticulously below in conjunction with the accompanying drawings and preferred embodiments of the present invention, but the protection scope of the present invention is not limited to the following specific embodiments.

[0068] Unless otherwise defined, all professional terms used below have the same meanings as those generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention.

[0069] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0070] like Figure 1-4 and Fig. 9 , 1. Motor; 2. Feeding port; 3. Bionic intestine; 4. Spherical material container; 5. Water bath jacket; 51. Reactor tank; 6. Primary discharge mechanism; 7. Secondary discharge mechanism; 8. Coupling; 9. Glass window; 10. Stirring shaft; 11. Support base; 12. Connecting elbow; 13. Feeding duct; 14. Hemispherical shell; 15. Buckle; 16. Tightening knob; 21. Clamp; 22. Exhaust port; 23. First sampling port; 24. Second sampling port; 25. Third sampling port; 26. Water inlet; 27. Water outlet; 28. Pull-out baffle; 29. ​​Paddle; 30. Tightening sleeve one; 31. Tightening sleeve two, A. Feeding section; B. Spiral section; C: Outlet section.

[0071] The present application provides a spherical segmented feeding intestinal biomimetic reactor, including a tank body, a feeding device, a discharging device, a stirring device, a spherical material container 4, a biomimetic intestinal device and a temperature control device. The tank body includes a reactor tank body 51, which is installed vertically and fixed on a supporting base 11 by bolt connection. One or more feed ports 2 are arranged at the upper end of the reactor tank body 51; the feed port 2 is in the shape of a funnel, with an open funnel end and a sealed end, wherein the sealed end is connected to below the liquid level in the reactor tank body 51 through a feeding conduit 13, and is connected to the biomimetic intestinal 3 through a clamp 21. The feeding conduit 13 needs to be tightly connected to the reactor cover to prevent air leakage, and the open funnel end is sealed with a metal clamp and a rubber gasket. The metal clamp and rubber gasket are used for sealing, which are opened during feeding and sealed and locked under normal conditions to prevent biogas leakage.

[0072] The stirring device is located inside the reactor tank body 51 and is fixed to the upper end of the reactor tank body 51 by bolt connection; the stirring device includes a motor 1, a coupling 8 and a stirring shaft 10, the motor 1 is arranged outside the reactor tank body 51, and is connected to the stirring shaft 10 through the coupling 8, the stirring shaft 10 vertically passes through the outer shell of the reactor tank body 51 to the inside of the tank body, the stirring shaft 10 extends below the liquid level in the reactor tank body 51, and one or more paddles 29 are arranged on the stirring shaft 10.

[0073] The structure of the stirring shaft 10 is as follows Fig. 9 As shown, the blade surface of the paddle 29 is fan-shaped with a spiral blade layout, and is connected to the stirring shaft 10 through a fastening sleeve 1 30 and a fastening sleeve 2 31. The fastening sleeve 1 30 controls the angle between the blade 29 and the horizontal plane, and the fastening sleeve 2 31 controls the blade height of the paddle 29 and the direction of the blade in the horizontal direction of the stirring shaft 10. The paddles are distributed in the spiral section of the bionic intestine, which can effectively mix the materials and promote the reaction. The propeller blade length is sufficient to not collide with the outer wall of the intestine, and the blade surface is fan-shaped, which can fully disturb the fermentation liquid, ensure the stirring shear force without damaging the microorganisms. The blade can adjust the angle through the fastening sleeve to ensure that it is parallel to the direction of the intestinal bend, further improving the stirring effect; the rotation speed of the stirrer can be adjusted to meet the stirring requirements under different reaction conditions. The stirring shaft 10 is driven to rotate by the motor 1 to accelerate the mixing of the inoculated mud around the bionic intestine 3, improve the heat transfer and mass transfer rate, maintain the stability of the microbial community structure, promote the reaction, and reduce the generation of by-products. According to the reaction conditions at different stages, the stable performance of anaerobic fermentation to produce methane is maintained.

[0074] The bionic intestinal device includes a bionic intestinal 3, which is a mesh hollow tube structure. A spirally wrapped stirring device is arranged inside the tank body, and is arranged in one or more forms, close to the inner wall of the reactor tank body 51, and connected to the feeding device and the discharging device through clamps respectively; the inlet of the feeding device adopts an open trumpet shape to ensure smooth entry of materials.

[0075] The reaction area of ​​the bionic intestinal device is as follows Fig.10 As shown, the design simulates the biological intestine and is spirally distributed, and the shape includes a starting section A, a spiral section B and an exit section C;

[0076] The starting section A is connected to the feeding device, and the angle with the horizontal plane is 40-90°;

[0077] The spiral section B is set along the inner wall of the tank, and the angle between any part of the spiral section and the horizontal plane in the falling direction is 30-60°

[0078] The outlet section C is connected to the discharging device, and the angle between the outlet section C and the horizontal plane is 40-90°.

[0079] The spiral section B is arranged close to the inner wall of the tank and can be wound in one section, one circle or multiple circles to meet the reaction time required for the degradation of different materials according to the requirements of the height-to-diameter ratio and residence time of different bioreactors.

[0080] The spherical material container 4 enters the spiral section along the starting section. Due to the design of the spiral section, the spherical material container can fall at a certain speed, and there is no jamming when passing through the bend, and it passes smoothly and continues to flow downward. The bending angle does not restrict the flow of the spherical material container, and no abnormality is observed. Finally, it falls to the outlet section, reaches the set residence time, and discharges the material after fermentation.

[0081] The relationship between the bionic intestinal coil diameter, number of turns and angle and the tank height-to-diameter ratio is:

[0082]

[0083] Where h is the height of the tank (vertical height of the bionic intestine), n is the number of spiral turns, θ is the spiral angle, which is the angle between the spiral segment and the horizontal plane; d is the diameter of the bionic intestine, and D is the spiral diameter of the pipeline inside the cylinder.

[0084] The ratio of the total height of the spiral segment in the vertical direction to the tank diameter is within (1.7~4):1. When the ratio is 4:1, it means that the bionic intestinal spiral segment is higher, which means more spiral turns, which can be used to degrade difficult-to-degrade materials such as straw, increase the contact time in the intestine, and make it fully degraded; when the ratio is 1.7:1, it means that the bionic intestinal spiral segment is shorter, which means fewer spiral turns, which can be used to degrade easily degradable materials such as sewage, livestock and poultry manure, etc., to ensure its degradation efficiency while improving the reaction efficiency.

[0085] The wall of the bionic intestine 3 is provided with a hollow structure, which can be designed and adjusted according to the characteristics of the fermentation material, and can increase the contact area between the biogas slurry and the material. In the tank, the material in the intestine is only distributed in the intestine and cannot be directly dispersed in the external liquid, preventing the material from floating on the surface of the reaction liquid and inhibiting the gas generated by the material reaction from overflowing, which in turn causes the material to block the exhaust hole, inhibit gas production, and cause the system to "instability".

[0086] The bionic intestine 3 and the spherical material container 4 are made of at least one of metal or plastic;

[0087] The cross-sectional diameter of the tubular structure of the bionic intestine 3 is 1 / 10-1 / 4 of the inner diameter of the bionic reactor.

[0088] The diameter of the spherical material container 4 is 2-5 mm smaller than the diameter of the bionic intestine 3. The spherical material container 4 includes a hemispherical shell 14, a fastening knob 16, and a buckle 15. The two hemispherical shells 14 are connected by the fastening knob 16 and then opened and closed by the buckle 15.

[0089] The material is arranged inside the spherical material container 4 and is fed into the bionic reactor through the spherical material container 4 along the feeding device.

[0090] For feeding, a spherical material container 4 may be placed every day, or a spherical material container 4 may be placed every half day as required, so as to adapt to different organic loads of the feed and realize semi-continuous automatic feeding.

[0091] A water bath jacket is provided outside the reaction tank body to maintain a stable temperature inside the reaction tank. The water bath jacket is heated or cooled by a hot water circulation system. Hot water is introduced from the water inlet 26 and discharged from the water outlet 27, so that the fermentation process is carried out within the optimal temperature range to ensure the activity of the microorganisms and the fermentation efficiency. The temperature control of the water bath jacket is accurate and can be adjusted according to the fermentation requirements, thereby improving the overall performance of the system. A glass window 9 is also provided on the reactor tank body 51 to observe the internal conditions of the reactor.

[0092] The discharge device is located at the lower end of the solid phase separation of the reaction tank, and includes a primary discharge mechanism 6 and a secondary discharge mechanism 7. The primary discharge mechanism 6 and the secondary discharge mechanism 7 are connected by a connecting elbow 12; a pull-out baffle 28 is designed between the primary discharge mechanism 6 and the bionic intestine 3. When discharging, the pull-out baffle 28 is used to discharge the spherical material container 4, and the microbial mud continues to remain in the fermentation tank, ensuring efficient biogas production while maintaining the stability of the fermentation system.

[0093] The upper end of the reactor tank body 51 is provided with an exhaust port 22 for exhausting the gas produced during the reaction process, and is also provided with a first sampling port 23, a second sampling port 24, and a third sampling port 25 for collecting samples.

[0094] The inoculated mud is stored in the reactor tank body 51. During the reaction, the solid phase and the liquid phase are separated at the lower end of the reactor tank, and the gas phase is separated at the upper end of the reactor tank.

[0095] Embodiment 1:

[0096] This embodiment adopts Figure 1-3 In the spherical segmented feeding intestine bionic reactor shown, the bionic intestine 3 adopts a metal hollow mesh structure, and its cross-sectional diameter ranges from 20 to 40 mm. The pore diameter range of the bionic intestine 3 is 5 to 10 mm, and the wall thickness is 1 to 2 mm.

[0097] The spherical material container has a spherical structure and adopts a hollow metal mesh with a diameter range of 15-35mm and a pore diameter range of 1-2mm.

[0098] The inoculum used in this example was taken from the discharge port of a restaurant waste treatment plant. The anaerobic digestion temperature of the restaurant waste treatment plant was maintained at 55°C, and a water bath was used for temperature cycling during the experiment. The inoculum was left for 2 days without adding any substance to eliminate background gas.

[0099] In this example, Miscanthus materials were collected on a farm and naturally air-dried at room temperature for 30 days. Subsequently, the Miscanthus stems were crushed to a particle size suitable for passing through a 20-mesh screen to promote particle size reduction. At the same time, the Miscanthus leaves were mechanically shredded to reduce their physical size to a diameter of about 1-1.5 cm. All samples were carefully packaged and stored at 4°C before use.

[0100] The reaction specifically comprises the following steps:

[0101] (1) Pretreatment of inoculum mud: Add inoculum mud into the tank of the vertical phase-separated intestinal biomimetic reactor; before use, the inoculum mud should be sieved to remove impurities larger than 10 mm, and the VS ratio (mass ratio) in the inoculum mud should be 0.7%-3%;

[0102] (2) Start-up phase with 0.5gVSL -1 d -1 The organic load of the material is put into the bionic intestinal device from the feeding device, and the stirring device is turned on for stirring. The material falls along the bionic intestinal tract and is fully mixed with the microorganisms in the inoculated mud, and gas production begins, thereby increasing the organic load and enriching the advantageous organic load rate of the fermentation material; the solid retention time is 8 days;

[0103] (3) After the inoculation sludge culture is completed, the organic load is increased by 0.5 VSL each time -1 d -1 , put in materials for continuous reaction, and when the materials fall to the connection between the bionic intestine device and the discharging device until the reaction is completed, open the discharging device and collect the reacted materials.

[0104] Material falling state: The spherical material container 4 first enters the starting section, and then enters the spiral section along the starting section. Due to the design of the spiral section, the spherical material container can be ensured to fall at a certain speed, and there is no jamming phenomenon when passing through the bend, and it passes smoothly and continues to flow downward. No abnormal situation is observed, and finally falls to the outlet section, and the material is discharged after the set residence time is reached and fermented.

[0105] During the experiment, no agglomeration or floating of the reaction materials was observed. After the reaction was completed, it was observed that the reaction materials were uniformly mixed inside and there was no incomplete reaction.

[0106] The experimental results show that the reaction materials are fully mixed during the flow process, ensuring the completeness and effectiveness of the reaction.

[0107] Experimental results:

[0108] The gas production efficiency in the microbial reaction was tested, and the test results were as follows: Figure 7 , 8 Shown by the middle curve.

[0109] Lignocellulose is composed of lignin, cellulose and hemicellulose. The degradation rate of lignocellulose is one of the indicators to characterize the biodegradation characteristics of biomass after the same pretreatment and the same reaction time. The degradation of lignocellulose in the fermented material was detected by a semi-automatic cellulose analyzer and a fiber detergent method. The test results are shown in Table 1. The detection methods include:

[0110] Sample defatting: accurately weigh 0.5 g of fermentation material into a 150 mL conical flask, add 30 mL of ethanol-ether (volume ratio of 15:1) mixture, place in an ultrasonic cleaner for ultrasonic cleaning at room temperature for 1 h, take out and transfer the sample to a G2 glass sand core crucible filled with 2 g of quartz sand for filtration, wash the conical flask and residue with ethanol, and then wash with 5 mL of acetone.

[0111] Acid detergent fiber determination: Add about 100mL 2mol / L hydrochloric acid detergent to the degreased sample in the hot extraction unit, add 3 to 5 drops of n-octanol, quickly heat to boiling, and adjust the power to maintain a slightly boiling state for 1h. After the heating is completed, filter immediately and rinse the tube wall and residue with water above 90°C until the filtrate is free of foam. Place the hot-extracted sample and crucible on the cold extraction unit of the fiber analyzer, rinse the residue with acetone 3 times, and ensure that the residue is fully mixed with acetone until the filtrate is colorless. After drying, put the crucible and residue in a (105±2)℃ oven and dry for 3 to 4h to constant weight and then weigh. Place the crucible and residue in a muffle furnace and weigh after ash treatment at (510±10)℃ for 3h. The cellulose degradation rate data obtained under the above conditions are shown in Table 1.

[0112] The lignocellulose components of the Miscanthus material before treatment were as follows: hemicellulose 30.87%, cellulose 39.40%, lignin 10.29% by mass

[0113] The lignocellulose components in the fermented material are as follows: hemicellulose 1.88%, cellulose 5.93%, lignin 2.32% by mass

[0114] It can be clearly seen from Figures 7 and 8 that when the organic load was relatively low in the early stage (OLR≦2gvs / (L*d)), there was no significant difference between the two. However, at a higher organic load, the device of the present application showed a better gas production effect, and the carrying capacity for the organic load was also increased. After the same high organic load, it can be found that the device of the present application still operates well, and the device without the spherical material carrying device cannot withstand the high organic load, which leads to the collapse of the reaction system.

[0115] Table 1 Cellulose degradation rate data of examples and comparative examples

[0116]

[0117] Embodiment 2:

[0118] This embodiment uses a spherical segmented feeding intestinal biomimetic reactor. The only difference from Embodiment 1 is that the biomimetic intestine 3 in this embodiment uses a metal hollow structure such as Figure 4 As shown, its cross-sectional diameter ranges from 20 to 40 mm, the pore diameter ranges from 5 to 10 mm, and the wall thickness is 1 to 2 mm. The spherical material container is a spherical structure, using a hollow metal mesh, with a diameter range of 15 to 35 mm and a pore diameter range of 1 to 2 mm.

[0119] The material used in this application is corn stalks, which are dried for one month and then broken and sieved into 3-5 mm blocks for testing.

[0120] The reaction specifically comprises the following steps:

[0121] (1) Pretreatment of inoculum mud: Add inoculum mud into the tank of the vertical phase-separated intestinal biomimetic reactor; before use, the inoculum mud should be sieved to remove impurities larger than 10 mm, and the VS ratio (mass ratio) in the inoculum mud should be 0.7%-3%;

[0122] (2) Start-up phase with 0.5gVSL -1 d -1 The organic load of the material is put into the bionic intestinal device from the feeding device, and the stirring device is turned on for stirring. The material falls along the bionic intestinal tract and is fully mixed with the microorganisms in the inoculated mud, and gas production begins, thereby increasing the organic load and enriching the advantageous organic load rate of the fermentation material; the solid retention time is 12 days;

[0123] (3) After the inoculation sludge culture is completed, the organic load is increased by 0.5 VSL each time -1 d -1 , put in materials for continuous reaction, and when the materials fall to the connection between the bionic intestine device and the discharging device until the reaction is completed, open the discharging device and collect the reacted materials.

[0124] Comparative Example 1:

[0125] This comparative example adopts a vertical phase-separated intestinal biomimetic reactor without a spherical material container, and its structural schematic diagram is shown in FIG. Figure 5 Except that the spherical material carrying device is not included, the rest of the parts are consistent with those of Example 1.

[0126] When the comparative example was reacted, the added materials and the seeding mud used were consistent with those in Example 1, and the other conditions were also consistent.

[0127] The degradation of lignocellulose in the fermented material was detected, and the test results are shown in Table 1.

[0128] The gas production efficiency in the reaction was tested, and the test results were as follows: Figure 7 shown.

[0129] Comparative Example 2:

[0130] This comparative example adopts a bionic reactor without a spherical material carrying device and an intestinal structure, and its structural schematic diagram is shown in FIG. Figure 6 Except that the spherical material carrying device and the intestinal structure are not included, the rest of the parts are consistent with those of Example 1.

[0131] This comparative example carried out an anaerobic fermentation test, and the added materials and the inoculated mud used were consistent with those in Example 1.

[0132] The degradation of lignocellulose in the fermented material was detected, and the test results are shown in Table 1.

[0133] The gas production efficiency in the microbial reaction was tested, and the test results were as follows: Figure 8 shown.

[0134] from Figure 7 , 8It can be seen that in the early period of time when the materials were just put in, there was no obvious difference between the curves of Example 1 and Comparative Examples 1 and 2. As the reaction proceeds and the materials are gradually added, the advantages of the bionic intestine + spherical material container begin to emerge, and the gas production begins to increase compared with the tank without adding the bionic intestine. At about 120 days, the gas production is about 2.2 times that of the tank without adding the bionic intestine, which is slightly higher than the gas production after adding the intestine. In the entire fermentation process, the gas production of the bionic intestine is higher than that of the tank without adding the bionic intestine and the tank with only adding the intestine. In the later stage of the reaction, the bionic intestine + spherical material device can withstand a larger organic load without "instability", which reflects the excellent effect of the device on the material reaction.

Claims

1. A spherical segmented feeding intestinal biomimetic reactor, characterized in that: The bionic reactor comprises a tank body, a bionic intestinal device, a spherical material container (4), a feeding device, a discharging device and a stirring device; the bionic intestinal device and the stirring device are arranged inside the tank body, one end of the bionic intestinal device is connected to the feeding device, and the other end is connected to the discharging device; The bionic intestinal device comprises one or more bionic intestinals (3), wherein the bionic intestinals (3) are in the form of a hollow tube structure and a stirring device is arranged spirally around the intestinal tract; The spherical material container (4) is in the form of a hollow spherical structure, the diameter of which is smaller than the inner diameter of the bionic intestinal device and cannot escape from the hollow portion of the hollow tube structure; the material is placed inside the spherical material container (4) and is fed into the bionic reactor through the spherical material container (4) along the feeding device.

2. The bionic reactor according to claim 1, characterized in that: The tank body comprises a reactor tank body (51), and inoculated mud is stored in the reactor tank body (51).

3. The bionic reactor according to claim 2, characterized in that: The bionic intestine (3) is in a spiral shape, and the spiral shape includes a starting section, a spiral section, and an exit section; The starting section is connected to the feeding device, and the angle between the starting section and the horizontal plane is 40-90°; The spiral section is arranged along the inner wall of the tank, and the angle between the spiral section and the horizontal plane at any point in the falling direction is 30-60°; The outlet section is connected to the discharging device, and the angle between the outlet section and the horizontal plane is 40-90°.

4. The bionic reactor according to claim 2, characterized in that: The material of the bionic intestine (3) is at least one of metal or plastic, and the inner diameter of the bionic intestine (3) is 1 / 10 to 1 / 4 of the inner diameter of the bionic reactor.

5. The bionic reactor according to claim 2, characterized in that: The spherical material container (4) comprises a hemispherical outer shell (14), a fastening knob (16), and a buckle (15); the two hemispherical outer shells (14) are connected via the fastening knob (16) and are then opened and closed via the buckle (15).

6. The bionic reactor according to claim 5, characterized in that: The material of the spherical material container (4) is at least one of metal and plastic; the diameter of the spherical material container (4) is 2-5 mm smaller than the inner diameter of the bionic intestine (3).

7. The bionic reactor according to claim 2, characterized in that: The stirring device comprises a motor (1), a coupling (8) and a stirring shaft (10); the motor (1) is arranged outside the reactor tank (51) and is connected to the stirring shaft (10) via the coupling (8); the stirring shaft (10) vertically passes through the outer shell of the reactor tank (51) to the inside of the tank; the stirring shaft (10) extends below the liquid level of the inoculated mud in the reactor tank (51); and one or more stirring blades (29) are arranged on the stirring shaft (10).

8. The bionic reactor according to claim 7, characterized in that: The blade surface of the paddle (29) is fan-shaped and is connected to the stirring shaft (10) via a first fastening sleeve (30) and a second fastening sleeve (31). The first fastening sleeve (30) controls the angle between the blade of the paddle (29) and the horizontal plane, and the second fastening sleeve (31) controls the blade height of the paddle (29) and the orientation of the blade in the horizontal direction of the stirring shaft (10).

9. The bionic reactor according to claim 2, characterized in that The feeding device comprises one or more feeding ports (2), wherein the feeding port (2) is in the shape of a funnel, and has an open funnel end and a sealed end, wherein the sealed end is connected to below the liquid level of the inoculated mud in the reactor tank (51) through a feeding conduit (13), and is connected to the bionic intestine (3) through a clamp (21), and the open funnel end is sealed; The discharge device comprises a primary discharge mechanism (6) and a secondary discharge mechanism (7), the primary discharge mechanism (6) and the secondary discharge mechanism (7) being connected via a curved pipe (12); the primary discharge mechanism (6) is connected to the bionic intestine (3), and a draw-out baffle (28) is provided between the primary discharge mechanism (6) and the bionic intestine (3); The bionic reactor further comprises a temperature control system, the temperature control system comprising a water bath jacket (5) arranged outside the reactor tank body (51); the reactor tank body (51) is also provided with a glass window (9) and a support base (11).

10. A control method for the spherical segmented feeding intestinal bionic reactor according to any one of claims 1 to 9, characterized in that: The following steps are involved: (1) Adding inoculum mud into the tank of the spherical segmented feeding intestine biomimetic reactor; (2) placing the material into the spherical material container (4), and placing the material into the bionic intestinal device through the feeding device, and starting the stirring device to stir the material to cultivate the inoculated mud; (3) After the inoculated mud is cultivated, it is put into the spherical material container (4). After the reaction between the material and the inoculated mud is completed, the discharging device is opened to discharge the spherical material container (4) and collect the material therein.

11. The control method according to claim 10, characterized in that: Before adding the spherical material container (4) in step (3), an organic load is set and the material is quantitatively added according to the organic load; and when discharging the spherical material container (4) in step (3), only the spherical material container (4) is discharged, and the seeding mud is retained in the bionic reactor for repeated use.

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