A composition with anti-aging and whitening effects and its application

Abstract

The present invention relates to a medicine-food homologous composition with anti-aging and whitening effects and its application. The composition includes raspberry extract, guava extract, rose extract and licorice extract. The composition utilizes the active ingredients of natural plants to achieve anti-aging effects by inhibiting the activities of collagenase and elastase and promoting the generation of collagen and elastin; prevents aging caused by skin glycation by inhibiting the activity of α-glucosidase and directly inhibiting the generation of AGEs; achieves whitening effects by scavenging DPPH free radicals, inhibiting the activity of tyrosinase and reducing melanin production; the composition is formulated in a specific ratio to achieve synergistic effects, and further improves the whitening and anti-glycation effects while anti-aging, improving skin problems from multiple dimensions. The four natural ingredients used in the composition are all derived from medicine-food homology, and the extraction process does not contain organic reagents, which is green, environmentally friendly, safe and reliable.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant extraction, and relates to a composition with anti-aging and whitening effects and its application. Background Art

[0002] The skin is the largest organ of the human body, with important functions such as barrier protection, body temperature regulation, immune defense, perception, and excretion. Skin aging is caused by various internal and external factors, mainly manifested as skin elasticity relaxation, roughness, wrinkle proliferation, skin dullness, and pigmentation. Ultraviolet irradiation is the main external factor leading to skin aging. It can not only induce the skin to produce a large amount of reactive oxygen species (ROS), accelerate the skin aging process, but also promote the proliferation of melanocytes and stimulate melanin production.

[0003] During the skin aging process, the number of fibroblasts in the dermis decreases, and the synthesis ability of collagen and elastin in the extracellular matrix decreases, resulting in a thinner dermis, reduced skin elasticity, and wrinkle proliferation. Advanced glycation end products (AGEs) are substances formed by the reaction of reducing sugars with free amino groups in proteins, lipids, and nucleic acids, and play a key role in skin aging. AGEs form crosslinks with collagen and elastin in the skin, making the skin lose elasticity and flexibility, increasing wrinkles and relaxation. In addition, AGEs also activate inflammatory responses and oxidative stress, increase free radical generation, further damage skin cells and structures, and lead to dull skin color and impaired skin function.

[0004] Based on this, the purpose of the present invention is to provide a preparation method, product, and application of a medicine and food homologous composition with anti-aging and whitening effects. Summary of the Invention

[0005] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a composition with anti-aging and whitening effects and its application.

[0006] To achieve the purpose of this invention, the following technical solutions are adopted:

[0007] In the first aspect, the present invention provides a medicine and food homologous composition with anti-aging and whitening effects, and the composition includes raspberry extract, guava extract, rose extract, and licorice extract.

[0008] Rubus chingii Hu is the dried fruit of a Rosaceae plant, rich in active ingredients such as polyphenols and ellagic acid, and has the effects of tonifying the kidney, consolidating essence, reducing urination, and nourishing the liver and improving eyesight. It is a dual-effect food with both nutritional and medicinal values. Modern research shows that raspberry extract has significant antioxidant and anti-inflammatory effects.

[0009] Guava (Psidium guajava L.) is the fruit of the plant in the Myrtaceae family and has extremely high nutritional, health care, and pharmacological values. Guava polysaccharide is one of the most active components among them and has various biological functions such as anti-inflammatory, antidiarrheal, antihypertensive, and antidiabetic effects.

[0010] Rose flower (Rosa rugosa Thunb.) is the dried flower bud of the plant in the Rosaceae family and is rich in active ingredients such as polyphenols, flavonoids, and vitamin C, and has pharmacological effects such as promoting qi and relieving depression, and regulating qi and relieving pain. Modern research shows that rose extract can reduce melanin production and show significant anti-skin aging ability by inhibiting the level of matrix metalloproteinase-1 (MMP-1).

[0011] With the increasing requirements for the effectiveness, safety, and durability of anti-aging methods, preventing and alleviating skin aging through diet has become an inevitable trend. Raspberry, guava, rose flower, and licorice are all raw materials that are both medicine and food. Through specific proportion compatibility, this composition achieves synergistic effects, not only can improve skin health in multiple aspects and dimensions, but also can fundamentally prevent skin aging through internal regulation, achieving the effect of improving skin condition.

[0012] Preferably, the composition includes 10 - 30 parts by mass of raspberry extract, 5 - 10 parts by mass of guava extract, 10 - 30 parts by mass of rose extract, and 1 - 5 parts by mass of licorice extract.

[0013] The mass parts of the raspberry extract can be selected as 10 parts, 12 parts, 14 parts, 16 parts, 18 parts, 20 parts, 22 parts, 24 parts, 26 parts, 28 parts, 30 parts, etc.; the mass parts of the guava extract can be selected as 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, etc.; the mass parts of the rose extract can be selected as 10 parts, 12 parts, 14 parts, 16 parts, 18 parts, 20 parts, 22 parts, 24 parts, 26 parts, 28 parts, 30 parts, etc.; the mass parts of the licorice extract can be selected as 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc. Other specific point values within the above numerical range can be selected and will not be elaborated one by one here.

[0014] Preferably, the raspberry extract is prepared by a method including the following steps: mixing raspberry with water, performing ultrasonic extraction, filtering the extract through a filter bag and a ceramic membrane, concentrating, and drying to obtain it.

[0015] Preferably, the guava extract is prepared by a method including the following steps: mixing guava with water, performing reflux extraction, filtering through a filter bag and a ceramic membrane, concentrating, and drying to obtain it.

[0016] Preferably, the rose extract is prepared by a method comprising the following steps: mixing rose with water, subjecting to negative pressure cavitation extraction, filtering through a filter bag and a ceramic membrane, concentrating, and drying to obtain the rose extract.

[0017] Preferably, before the ultrasonic extraction, the raspberry and water mixture is also treated at 110 - 130 °C and 0.15 - 0.25 MPa for 5 - 10 min.

[0018] The temperature can be selected as 110 °C, 112 °C, 114 °C, 116 °C, 118 °C, 120 °C, 122 °C, 124 °C, 126 °C, 128 °C, 130 °C, etc.; the pressure can be selected as 0.15 MPa, 0.16 MPa, 0.17 MPa, 0.18 MPa, 0.19 MPa, 0.2 MPa, 0.21 MPa, 0.22 MPa, 0.23 MPa, 0.24 MPa, 0.25 MPa, etc.; the time can be selected as 5 min, 6 min, 7 min, 8 min, 9 min, 10 min, etc. Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0019] The power of the ultrasonic wave is 200 - 500 W, the frequency is 30 - 50 kHz, the temperature is 60 - 70 °C, and the time is 30 - 40 min.

[0020] The power of the ultrasonic wave can be selected as 200 W, 220 W, 250 W, 280 W, 300 W, 320 W, 350 W, 380 W, 400 W, 420 W, 450 W, 480 W, 500 W, etc.; the frequency can be selected as 30 kHz, 32 kHz, 34 kHz, 36 kHz, 38 kHz, 40 kHz, 42 kHz, 44 kHz, 46 kHz, 48 kHz, 50 kHz, etc.; the temperature can be selected as 60 °C, 61 °C, 62 °C, 63 °C, 64 °C, 65 °C, 66 °C, 67 °C, 68 °C, 69 °C, 70 °C, etc.; the time can be selected as 30 min, 31 min, 32 min, 33 min, 34 min, 35 min, 36 min, 37 min, 38 min, 39 min, 40 min, etc. Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0021] Preferably, the ratio of raspberry to water is 1 g : (15 - 20) mL.

[0022] Preferably, the ratio of guava to water is 1 g : (10 - 15) mL, and the reflux extraction time is 1 - 2 h.

[0023] Among them, the specific point values in (15 - 20) can all be selected from 15, 15.5, 16, 16.5, 17, 17.5, 18, 18.5, 19, 19.5, 20, etc., the specific point values in (10 - 15) can all be selected from 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5, 15, etc., and the time can be selected from 1h, 1.2h, 1.4h, 1.6h, 1.8h, 2h, etc. Other specific point values within the above numerical ranges can all be selected and will not be elaborated one by one here.

[0024] Preferably, the ratio of rose to water is 1g:(40 - 60)mL, the pressure of negative pressure cavitation extraction is 0.03 - 0.08 Mpa, the temperature is 50 - 60 °C, and the time is 1 - 1.5h.

[0025] Among them, the specific point values in (40 - 60) can all be selected from 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, etc., the pressure can be selected from 0.03 MPa, 0.04 MPa, 0.05 MPa, 0.06 MPa, 0.07 MPa, 0.08 MPa, etc., the temperature can be selected from 50 °C, 51 °C, 52 °C, 53 °C, 54 °C, 55 °C, 56 °C, 57 °C, 58 °C, 59 °C, 60 °C, etc., and the time can be selected from 1h, 1.1h, 1.2h, 1.3h, 1.4h, 1.5h, etc. Other specific point values within the above numerical ranges can all be selected and will not be elaborated one by one here.

[0026] Preferably, the filtration in raspberry extraction is carried out at 40 - 60 °C, such as 40 °C, 42 °C, 44 °C, 46 °C, 48 °C, 50 °C, 52 °C, 54 °C, 56 °C, 58 °C, 60 °C, etc. Other specific point values within the above numerical ranges can all be selected and will not be elaborated one by one here.

[0027] Preferably, the filtration in guava extraction is carried out at 30 - 40 °C, such as 30 °C, 31 °C, 32 °C, 33 °C, 34 °C, 35 °C, 36 °C, 37 °C, 38 °C, 39 °C, 40 °C, etc. Other specific point values within the above numerical ranges can all be selected and will not be elaborated one by one here.

[0028] Preferably, the filter bag filtration includes the first filter bag filtration and the second filter bag filtration.

[0029] Preferably, the aperture of the first filter bag is 400 - 600 mesh, such as 400 mesh, 420 mesh, 450 mesh, 480 mesh, 500 mesh, 520 mesh, 550 mesh, 580 mesh, 600 mesh, etc. Other specific point values within the above numerical ranges can all be selected and will not be elaborated one by one here.

[0030] Preferably, the pore size of the second filter bag is 5-10 μm, such as 5 μm, 6 μm, 7 μm, 8 μm, 9 μm, 10 μm, etc. Other specific point values within the above numerical range can be selected and will not be elaborated one by one here.

[0031] The pore size of the ceramic membrane used in raspberry extraction is 300-400 nm (such as 300 nm, 310 nm, 320 nm, 330 nm, 340 nm, 350 nm, 360 nm, 370 nm, 380 nm, 390 nm, 400 nm, etc.), the pore size of the ceramic membrane used in guava extraction is 100-200 nm (such as 100 nm, 110 nm, 120 nm, 130 nm, 140 nm, 150 nm, 160 nm, 170 nm, 180 nm, 190 nm, 200 nm, etc.), and the pore size of the ceramic membrane used in rose extraction is 50-100 nm (such as 50 nm, 60 nm, 70 nm, 80 nm, 90 nm, 100 nm, etc.). Other specific point values within the above numerical range can be selected and will not be elaborated one by one here.

[0032] In a second aspect, the present invention provides an application of the medicine-food homologous composition with anti-aging and whitening effects according to the first aspect in the preparation of a product with whitening, anti-aging or anti-glycation effects.

[0033] Preferably, the product includes food, health products or cosmetics.

[0034] Preferably, the addition amount of the medicine-food homologous composition in the product is 0.2-50%, such as 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 2%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, etc. Other specific point values within the above numerical range can be selected and will not be elaborated one by one here.

[0035] Compared with the prior art, the present invention has the following beneficial effects:

[0036] (1) The present invention respectively adopts specific extraction processes to effectively extract the active ingredients in raspberries, guavas and roses, and successfully obtains a composition with anti-aging, whitening and anti-glycation effects.

[0037] (2) The composition obtained by the preparation method of the present invention can significantly inhibit the activities of collagenase and elastase, and can promote the expression of zebrafish glue genes col1a1a and col1a1b.

[0038] (3) The IC50 value of the composition obtained by the preparation method of the present invention for scavenging DPPH free radicals is less than 30 μg / mL, and it can significantly inhibit tyrosinase activity and zebrafish melanogenesis.

[0039] (4) The IC50 value of the composition obtained by the preparation method of the present invention for inhibiting α-glucosidase activity is less than 30 μg / mL, and it can significantly inhibit the formation of advanced glycation end products (AGEs).

[0040] (5) The composition obtained by the preparation method of the present invention has a synergistic effect in the directions of anti-aging, whitening, and anti-glycation.

[0041] (6) The present invention uses RO water as the extraction solvent and does not use organic reagents throughout the process. It not only effectively retains the biological activities of the medicine and food homologous plants but also expands their application scope in health products, having significant market potential and application value. Moreover, the method has strong coherence, high extraction efficiency, energy conservation and environmental protection, safety and reliability, and is conducive to industrial production and promotion. Description of the Drawings

[0042] Figure 1 It is the result diagram of the influence of the composition on the relative expression levels of zebrafish col1a1a and col1a1b genes. *, **, and *** indicate P < 0.05, P < 0.01, and P < 0.001 compared with the control group, respectively.

[0043] Figure 2 It is the result diagram of the influence of the composition on zebrafish melanogenesis. *** indicates P < 0.001 compared with the control group.

[0044] Figure 3 It is the stereomicroscopic observation diagram of the influence of the composition on zebrafish melanogenesis. Detailed Embodiments

[0045] The technical solutions of the present invention will be further described below through specific embodiments. Those skilled in the art should understand that the embodiments are only for helping to understand the present invention and should not be regarded as specific limitations to the present invention.

[0046] The sources of the active ingredients contained in the products involved in the following examples and comparative examples are as follows (only the active ingredients are shown, and the necessary auxiliary ingredients contained in other commercially available raw materials are not described in detail):

[0047] The licorice extract was purchased from Guangdong Qingyunshan Pharmaceutical Co., Ltd., and the glycyrrhizic acid content was ≥3%.

[0048] Preparation Example 1-1

[0049] This preparation example provides a preparation method of raspberry extract, and the preparation method includes:

[0050] (1) Crush raspberries and mix with water at a ratio of 1 g:18 mL. After treating at 120 °C and 0.2 MPa for 8 min, perform ultrasonic extraction at 65 °C for 35 min under ultrasonic conditions of 300 W and 40 kHz to obtain an extract.

[0051] (2) Cool the extract to 50 °C, then sequentially filter through 600-mesh and 5-μm filter bags, and then filter through a 350-nm ceramic membrane, concentrate to a solid content of 15%, sterilize, and dry to obtain the product.

[0052] Preparation Example 1-2

[0053] This preparation example provides a method for preparing a raspberry extract, and the preparation method includes:

[0054] (1) Crush raspberries and mix with water at a ratio of 1 g:15 mL. After treating at 110 °C and 0.15 MPa for 10 min, perform ultrasonic extraction at 70 °C for 30 min under ultrasonic conditions of 200 W and 30 kHz to obtain an extract.

[0055] (2) Cool the extract to 40 °C, then sequentially filter through 600-mesh and 5-μm filter bags, and then filter through a 300-nm ceramic membrane, concentrate to a solid content of 17%, sterilize, and dry to obtain the product.

[0056] Preparation Example 1-3

[0057] This preparation example provides a method for preparing a raspberry extract, and the preparation method includes:

[0058] (1) Crush raspberries and mix with water at a ratio of 1 g:20 mL. After treating at 130 °C and 0.25 MPa for 5 min, perform ultrasonic extraction at 60 °C for 40 min under ultrasonic conditions of 500 W and 50 kHz to obtain an extract.

[0059] (2) Cool the extract to 60 °C, then sequentially filter through 600-mesh and 5-μm filter bags, and then filter through a 400-nm ceramic membrane, concentrate to a solid content of 20%, sterilize, and dry to obtain the product.

[0060] Preparation Example 1-4

[0061] This preparation example provides a method for preparing a raspberry extract, and the difference from Preparation Example 1-1 is only that step (2) is "Cool the extract to 50 °C, then sequentially filter through 600-mesh and 5-μm filter bags, and then filter through a 200-nm ceramic membrane, concentrate to a solid content of 15%, sterilize, and dry to obtain the product", and other operations remain unchanged.

[0062] Preparation Example 1-5

[0063] This preparation example provides a method for preparing raspberry extract, which is only different from Preparation Example 1-1 in that step (2) is "cool the extract to 50 °C, then filter it through a 600-mesh and a 5-μm filter bag in sequence, and then filter it through a 500-nm ceramic membrane, concentrate it to a solid content of 15%, sterilize it, and dry it to obtain the product", and other operations remain unchanged.

[0064] Preparation Example 1-6

[0065] This preparation example provides a method for preparing raspberry extract, which is only different from Preparation Example 1-1 in that step (1) is "crush the raspberries and mix them with water at a ratio of 1 g:18 mL of raspberries to water, treat them at 120 °C and 0.2 MPa for 8 min, and then extract them at 65 °C for 35 min to obtain an extract".

[0066] Preparation Example 1-7

[0067] This preparation example provides a method for preparing raspberry extract, which is only different from Preparation Example 1-1 in that step (1) is "crush the raspberries and mix them with water at a ratio of 1 g:18 mL of raspberries to water, extract them ultrasonically at 65 °C for 35 min under ultrasonic conditions of 300 W and 40 kHz to obtain an extract", and other operations remain unchanged.

[0068] Preparation Example 1-8

[0069] This preparation example provides a method for preparing raspberry extract, which is only different from Preparation Example 1-1 in that step (1) is "crush the raspberries and mix them with water at a ratio of 1 g:18 mL of raspberries to water, treat them at 120 °C and 0.2 MPa for 8 min, and then extract them under negative pressure at 65 °C for 35 min with a negative pressure cavitation treatment pressure of 0.05 MPa to obtain an extract", and other operations remain unchanged.

[0070] Preparation Example 1-9

[0071] This preparation example provides a method for preparing raspberry extract, which is only different from Preparation Example 1-1 in that step (1) is "crush the raspberries and mix them with water at a ratio of 1 g:18 mL of raspberries to water, treat them at 120 °C and 0.2 MPa for 8 min, and then extract them by stirring at 65 °C for 35 min with a stirring speed of 80 r / min to obtain an extract", and other operations remain unchanged.

[0072] Preparation 1-10

[0073] This Preparation Example provides a method for preparing raspberry extract, which is only different from Preparation Example 1-1 in that step (1) is "crush raspberries and mix with water at a ratio of 1 g:18 mL, treat at 120 °C and 0.2 MPa for 8 min, then heat under reflux for extraction for 35 min, and extract twice to obtain the extract solution", and other operations remain unchanged.

[0074] Preparation Example 2-1

[0075] This Preparation Example provides a method for preparing guava extract, and the preparation method includes:

[0076] (1) Crush guavas and mix with water at a ratio of 1 g:12 mL, heat under reflux for extraction for 1.5 h, and extract twice;

[0077] (2) After cooling to 35 °C, filter successively through a 600-mesh and a 5-μm filter bag, then filter through a 140-nm ceramic membrane, concentrate to a solid content of 10%, sterilize, and dry to obtain the product.

[0078] Other operations remain unchanged.

[0079] Preparation Example 2-2

[0080] This Preparation Example provides a method for preparing guava extract, and the preparation method includes:

[0081] (1) Crush guavas and mix with water at a ratio of 1 g:10 mL, heat under reflux for extraction for 1 h, and extract twice;

[0082] (2) After cooling to 35 °C, filter successively through a 600-mesh and a 5-μm filter bag, then filter through a 100-nm ceramic membrane, concentrate to a solid content of 11%, sterilize, and dry to obtain the product.

[0083] Preparation Example 2-3

[0084] This Preparation Example provides a method for preparing guava extract, and the preparation method includes:

[0085] (1) Crush guavas and mix with water at a ratio of 1 g:15 mL, heat under reflux for extraction for 2 h, and extract twice;

[0086] (2) After cooling to 35 °C, filter successively through a 600-mesh and a 5-μm filter bag, then filter through a 200-nm ceramic membrane, concentrate to a solid content of 12%, sterilize, and dry to obtain the product.

[0087] Preparation Example 3-1

[0088] This Preparation Example provides a method for preparing rose extract, and the preparation method includes:

[0089] (1) Crush the roses and mix with water at a ratio of 1 g:45 mL, and perform negative pressure cavitation extraction at 55 °C for 1.2 h with a pressure of 0.05 MPa;

[0090] (2) After cooling to 40 °C, pass through 600-mesh and 5-μm filter bags in sequence, then filter through a 80-nm ceramic membrane, concentrate to a solid content of 8%, sterilize, and dry to obtain the product.

[0091] Preparation Example 3-2

[0092] This preparation example provides a method for preparing rose extract, and the preparation method includes:

[0093] (1) Crush the roses and mix with water at a ratio of 1 g:40 mL, and perform negative pressure cavitation extraction at 50 °C for 1.5 h with a pressure of 0.08 MPa;

[0094] (2) After cooling to 40 °C, pass through 600-mesh and 5-μm filter bags in sequence, then filter through a 50-nm ceramic membrane, concentrate to a solid content of 9%, sterilize, and dry to obtain the product.

[0095] Preparation Example 3-3

[0096] This preparation example provides a method for preparing rose extract, and the preparation method includes:

[0097] (1) Crush the roses and mix with water at a ratio of 1 g:60 mL, and perform negative pressure cavitation extraction at 60 °C for 1 h with a pressure of 0.03 MPa;

[0098] (2) After cooling to 40 °C, pass through 600-mesh and 5-μm filter bags in sequence, then filter through a 100-nm ceramic membrane, concentrate to a solid content of 10%, sterilize, and dry to obtain the product.

[0099] Preparation Example 3-4

[0100] This preparation example provides a method for preparing rose extract, and the only difference from Preparation Example 3-1 is that step (1) is "crush the roses and mix with water at a ratio of 1 g:45 mL, perform negative pressure cavitation extraction at 30 °C for 1.2 h with a pressure of 0.05 MPa". Other operations remain unchanged.

[0101] Preparation Example 3-5

[0102] This preparation example provides a method for preparing rose extract, and the only difference from Preparation Example 3-1 is that step (1) is "crush the roses and mix with water at a ratio of 1 g:45 mL, perform negative pressure cavitation extraction at 80 °C for 1.2 h with a pressure of 0.05 MPa". Other operations remain unchanged.

[0103] Preparation Example 3-6

[0104] This preparation example provides a method for preparing rose extract, which is only different from Preparation Example 3-1 in that step (1) is "crush the rose and mix it with water, the ratio of rose to water is 1 g: 20 mL, carry out negative pressure cavitation extraction at 55 °C for 1.2 h, and the pressure is 0.05 MPa". Other operations remain unchanged.

[0105] Preparation Example 3-7

[0106] This preparation example provides a method for preparing rose extract, which is only different from Preparation Example 3-1 in that step (1) is "crush the rose and mix it with water, the ratio of rose to water is 1 g: 80 mL, carry out negative pressure cavitation extraction at 55 °C for 1.2 h, and the pressure is 0.05 MPa". Other operations remain unchanged.

[0107] Preparation Example 3-8

[0108] This preparation example provides a method for preparing rose extract, which is only different from Preparation Example 3-1 in that step (1) is "crush the rose and mix it with water, the ratio of rose to water is 1 g: 45 mL, carry out constant temperature stirring extraction at 55 °C for 1.2 h, and the rotation speed is 80 rpm", and other operations remain unchanged.

[0109] Preparation Example 3-9

[0110] This preparation example provides a method for preparing rose extract, which is only different from Preparation Example 3-1 in that step (1) is "crush the rose and mix it with water, the ratio of rose to water is 1 g: 45 mL, carry out heating reflux extraction for 1.2 h", and other operations remain unchanged.

[0111] Preparation Example 3-10

[0112] This preparation example provides a method for preparing rose extract, which is only different from Preparation Example 3-1 in that step (1) is "crush the rose and mix it with water, the ratio of rose to water is 1 g: 45 mL, carry out ultrasonic extraction at 55 °C for 1.2 h, and the ultrasonic frequency is 40 kHz", and other operations remain unchanged.

[0113] Example 1

[0114] This example provides a medicated and edible homologous composition with anti-aging and whitening effects. The composition includes 20 parts of the raspberry extract described in Preparation Example 1-1, 8 parts of the guava extract described in Preparation Example 2-1, 20 parts of the rose extract described in Preparation Example 3-1, and 3 parts of the licorice extract.

[0115] The preparation method is: physically mix the preparation raw materials evenly to obtain it.

[0116] Example 2

[0117] This example provides a medicine-food homologous composition with anti-aging and whitening effects. The composition includes 10 parts of the raspberry extract described in Preparation Example 1-2, 10 parts of the guava extract described in Preparation Example 2-2, 12 parts of the rose extract described in Preparation Example 3-2, and 5 parts of the licorice extract.

[0118] The preparation method is: physically mix the preparation raw materials evenly to obtain.

[0119] Example 3

[0120] This example provides a medicine-food homologous composition with anti-aging and whitening effects. The composition includes 30 parts of the raspberry extract described in Preparation Example 1-3, 5 parts of the guava extract described in Preparation Example 2-3, 30 parts of the rose extract described in Preparation Example 3-3, and 2 parts of the licorice extract.

[0121] The preparation method is: physically mix the preparation raw materials to obtain.

[0122] Examples 4-10

[0123] This example provides 7 kinds of medicine-food homologous compositions with anti-aging and whitening effects. The difference from Example 1 is only that the raspberry extract described in Preparation Example 1-1 is respectively and equally replaced with the raspberry extracts described in Preparation Example 1-4, Preparation Example 1-5, Preparation Example 1-6, Preparation Example 1-7, Preparation Example 1-8, Preparation Example 1-9, and Preparation Example 1-10, and other components and contents remain unchanged.

[0124] The preparation method refers to Example 1.

[0125] Examples 11-17

[0126] This example provides 7 kinds of medicine-food homologous compositions with anti-aging and whitening effects. The difference from Example 1 is only that the rose extract described in Preparation Example 3-1 is respectively and equally replaced with the rose extracts described in Preparation Example 3-4, Preparation Example 3-5, Preparation Example 3-6, Preparation Example 3-7, Preparation Example 3-8, Preparation Example 3-9, and Preparation Example 3-10, and other components and contents remain unchanged.

[0127] The preparation method refers to Example 1.

[0128] Comparative Example 1

[0129] This comparative example provides a medicine-food homologous composition with anti-aging and whitening effects. The difference from Example 1 is only that it does not contain the raspberry extract, and the reduced mass is proportionally distributed to the masses of the guava extract, rose extract, and licorice extract, and other aspects remain unchanged.

[0130] The preparation method refers to Example 1.

[0131] Comparative Example 2

[0132] This comparative example provides a medicine-food homologous composition with anti-aging and whitening effects. The difference from Example 1 is only that it does not contain guava extract, and the reduced mass is proportionally distributed to the masses of raspberry extract, rose extract, and licorice extract, with other conditions remaining unchanged.

[0133] The preparation method refers to Example 1.

[0134] Comparative Example 3

[0135] This comparative example provides a medicine-food homologous composition with anti-aging and whitening effects. The difference from Example 1 is only that it does not contain rose extract, and the reduced mass is proportionally distributed to the masses of raspberry extract, guava extract, and licorice extract, with other conditions remaining unchanged.

[0136] The preparation method refers to Example 1.

[0137] Comparative Example 4

[0138] This comparative example provides a medicine-food homologous composition with anti-aging and whitening effects. The difference from Example 1 is only that it does not contain licorice extract, and the reduced mass is proportionally distributed to the masses of raspberry extract, guava extract, and rose extract, with other conditions remaining unchanged.

[0139] The preparation method refers to Example 1.

[0140] Test Example 1

[0141] Test for the inhibitory effect of collagenase

[0142] Prepare a tetracycline hydrochloride solution (positive control) with a concentration of 1 mg / mL and the test sample solution. Add 140 μL of collagenase solution with a concentration of 0.2 mg / mL and 60 μL of the 1 mg / mL test sample solution to a 96-well plate. At the same time, set up a 100% enzyme activity control group, that is, use 60 μL of PBS to replace the test sample solution, and the blank control group and the sample blank group use the same volume of buffer salt to replace. After incubating at 37 °C for 20 min, add 40 μL of the substrate FALGPA buffer salt solution, and measure the absorbance at 330 nm at 0 min and 20 min.

[0143] Table 1

[0144]

[0145] Calculate the inhibition rate of collagenase according to the following formula:

[0146]

[0147] ΔA330B = the difference between the absorbance values at two time points (0 min and 20 mins) of the control group.

[0148] ΔA330A = the difference between the absorbance values at two time points (0 min and 20 mins) of the blank group.

[0149] ΔA330D = the difference between the absorbance values at two time points (0 min and 20 mins) of the sample group.

[0150] ΔA330C = the difference between the absorbance values at two time points (0 min and 20 mins) of the sample blank group.

[0151] Each group was measured 3 times on average, and the average value was taken to calculate the collagenase inhibition rate value. The results are shown in Table 2.

[0152] Table 2

[0153]

[0154]

[0155] It can be seen from the data in Table 2 that the composition described in this application has a good inhibitory effect on collagenase. The preparation methods of raspberry extract and rose extract will also affect the effect of the composition, and raspberry extract, guava extract, rose extract and licorice extract have a certain synergistic effect on the above-mentioned effects, cooperate with each other in the above-mentioned effects, and are indispensable.

[0156] Test Example 2

[0157] Elastase Inhibition Effect Test

[0158] Add 0.03125 U / mL enzyme solution and PBS solution, 2 mg / mL sivelestat sodium solution (positive control) solution or 2 mg / mL sample solution to a 96-well plate. After incubating at 37 °C for 10 min, add 100 μmoL / L of 0.1 mM DMSO solution. The total reaction volume is 230 μL. At the same time, set a 100% enzyme activity control well, and replace 230 μL of the blank control with an equal volume of PBS. After incubating at 37 °C for 30 min, measure the absorbance value at 405 nm. Each group was measured 3 times on average, and the average value was taken to calculate the elastase inhibition rate value. The results are shown in Table 3.

[0159] Sample blank group: 70 μL of elastase + 50 μL of PBS + 10 μL of sample solution + 100 μL of DMSO solution

[0160] Sample group: 120 μL of PBS + 10 μL of sample solution + 100 μL of DMSO solution

[0161] Control group: 70 μL of elastase + 60 μL of PBS + 100 μL of DMSO solution

[0162] Control blank group: 130 μL of PBS + 100 μL of DMSO solution

[0163] Calculation method:

[0164]

[0165] Table 3

[0166] Group Elastase Inhibition Rate (%) Example 1 90.79 Example 2 85.47 Example 3 89.05 Example 4 79.84 Example 5 77.21 Example 6 75.15 Example 7 77.34 Example 8 64.21 Example 9 60.38 Example 10 76.54 Example 11 79.42 Example 12 76.95 Example 13 74.05 Example 14 76.34 Example 15 72.11 Example 16 65.97 Example 17 79.68 Comparative Example 1 29.11 Comparative Example 2 50.07 Comparative Example 3 45.71 Comparative Example 4 91.88 Preparation Example 1-1 80.19 Preparation Example 2-1 31.81 Preparation Example 3-1 59.13 Licorice Extract 2.18 Positive Control 99.07

[0167] As can be seen from the data in Table 3, the composition described in this application has a good inhibitory effect on elastase. The preparation methods of raspberry extract and rose extract will also affect the effect of the composition. Moreover, raspberry extract, guava extract, rose extract and licorice extract have a certain synergistic effect in the above-mentioned efficacy, cooperate with each other in the above-mentioned efficacy, and are indispensable.

[0168] Test Example 3

[0169] Antioxidant effect test

[0170] The concentration of the DPPH solution dissolved in absolute ethanol is 0.1 mg / mL. At the same time, vitamin C (positive control) and sample extract solution (concentration: 500 - 7.8125 μg / mL, diluted by the serial dilution method) are prepared. Mix 150 μL of the DPPH ethanol solution and 150 μL of the extract solution evenly, set a blank control group, react at room temperature in the dark for 30 min, shake well, measure the absorbance at 517 nm, and calculate the scavenging rate of the sample to DPPH free radicals according to the following formula:

[0171]

[0172] Sample blank group: 150 μL of sample solution + 150 μL of DPPH alcohol solution

[0173] Sample group: 150 μL of DPPH solution + 150 μL of sample solvent

[0174] Control group: 150 μL of sample solution + 150 μL of absolute ethanol

[0175] Control blank group: 150 μL of absolute ethanol + 150 μL of sample solvent

[0176] Using the concentration of the sample to be tested as the X-axis and the absorbance as the Y-axis, a scatter plot with a smoothed line and data markers was made. Three points in the upper, middle, and lower parts with a clearance rate of approximately 50% were selected to draw a straight line, and the straight-line equation was obtained to calculate the DPPH free radical scavenging IC50 value and the clearance rate of the sample. The results of the DPPH free radical scavenging IC50 value are shown in Table 4.

[0177] Table 4

[0178]

[0179]

[0180] As can be seen from the data in Table 4, the composition described in this application has good antioxidant effects. The preparation methods of raspberry extract and rose extract will also affect the effects of the composition, and raspberry extract, guava extract, rose extract, and licorice extract have certain synergistic effects on the above-mentioned effects and cooperate with each other in the above-mentioned effects, and none of them can be missing.

[0181] Test Example 4

[0182] Tyrosinase Inhibition Effect Test

[0183] Using a phosphate buffer solution with pH = 6.8 as a solvent, a 0.5 mg / mL L-tyrosine solution and a 100 μg / mL tyrosinase solution were prepared. Using water as a solvent, a 2 mg / mL arbutin (positive control) solution and a sample solution to be tested were prepared. The tyrosine solution, tyrosinase solution, and sample solution were mixed evenly. A blank control group was set and reacted at 37 °C for 30 minutes. The absorbance value was measured at 475 nm, and the inhibition rate of the sample on tyrosinase activity was calculated according to the following formula:

[0184]

[0185] Sample group: 100 μL of tyrosine solution + 50 μL of sample solution + 50 μL of tyrosinase solution. Sample blank group: 100 μL of tyrosine solution + 50 μL of sample solution + 50 μL of buffer solution. Control group: 100 μL of tyrosine solution + 50 μL of tyrosinase + 50 μL of buffer solution. Control blank group: 100 μL of tyrosine solution + 100 μL of buffer solution. Each group was measured 3 times on average, and the average value was taken to calculate the tyrosinase inhibition rate value. The results are shown in Table 5.

[0186] Table 5

[0187]

[0188]

[0189] As can be seen from the data in Table 5, the composition described in the present application has a good inhibitory effect on tyrosinase. The preparation methods of raspberry extract and rose extract will also affect the effect of the composition, and raspberry extract, guava extract, rose extract and licorice extract have a certain synergistic effect on the above-mentioned efficacy, cooperate with each other in the above-mentioned efficacy, and are indispensable.

[0190] Test Example 5

[0191] α-Glucosidase Inhibitory Effect Test

[0192] Using phosphate buffer solution with a pH of 6.8 as the solvent, prepare 5 mmol / L PNPG solution, 0.5 μg / mL α-glucosidase solution, 1 mol / L Na2CO3 solution, acarbose with an initial concentration of 5 μg / mL and sample solution with a concentration of 1000 μg / mL. Mix 50 μL of α-glucosidase solution with 50 μL of sample solution evenly, incubate at 37 °C for 10 min, then add 50 μL of PNPG solution to the mixed solution to start the reaction, mix well and incubate at 37 °C for 20 min. Finally, add 100 μL of sodium carbonate solution to the reaction solution to stop the reaction, and measure the absorbance value at a wavelength of 405 nm. Set up a blank control group.

[0193] Table 6

[0194]

[0195] Using the concentration of the sample to be measured as the X-axis and the absorbance as the Y-axis, make a scatter plot with a smooth line and data markers. Select 3 points at the upper, middle and lower levels with a clearance rate of about 50% to draw a straight line, obtain the straight line equation, and preliminarily estimate the IC50 value of the α-glucosidase activity inhibition rate of the sample and acarbose. Calculate the inhibition rate of the sample on α-glucosidase according to the following formula:

[0196]

[0197] The results are shown in Table 7.

[0198] Table 7

[0199] Group α-Glucosidase Inhibition IC50 (μg / mL) Example 1 18.03 Example 2 22.43 Example 3 19.07 Example 4 57.21 Example 5 30.15 Example 6 68.32 Example 7 70.11 Example 8 43.26 Example 9 47.92 Example 10 27.59 Example 11 69.06 Example 12 77.19 Example 13 79.32 Example 14 49.43 Example 15 78.04 Example 16 84.96 Example 17 65.42 Comparative Example 1 93.16 Comparative Example 2 47.57 Comparative Example 3 100.46 Comparative Example 4 26.02 Preparation Example 1-1 26.02 Preparation Example 2-1 149.08 Preparation Example 3-1 37.36 Licorice Extract 800.00 Positive Control 1.05

[0200] As can be seen from the data in Table 7, the composition described in the present application has a good inhibitory effect on α-glucosidase. The preparation methods of raspberry extract and rose extract will also affect the effect of the composition, and raspberry extract, guava extract, rose extract and licorice extract have a certain synergistic effect on the above-mentioned efficacy, cooperate with each other in the above-mentioned efficacy, and are indispensable.

[0201] Test Example 6

[0202] Anti - glycation test

[0203] Prepare a 1 mg / mL test sample solution with phosphate - buffered saline (50 mmol / L, pH 7.4). In a test tube, add 1 mL of the test sample, 0.8 mg / mL bovine serum albumin (BSA) solution, 200 mM glucose solution, and phosphate (PBS) buffer solution, and continuously heat at 60 °C for 24 h. Use aminoguanidine hydrochloride (AG) as a positive control. Fluorescent AGEs are measured with a fluorescence microplate reader. The excitation wavelength is 370 nm, the emission wavelength is 420 nm, and the content of fluorescent AGEs is expressed as fluorescence intensity AU.

[0204] Table 8

[0205] Sample Group (mL) Positive Control Group (mL) Blank Control (mL) PBS Phosphate Buffer 1 1 2 BSA Antibacterial Solution 1 1 1 Glu Solution 1 1 1 Test Sample Solution 1 - - Aminoguanidine Solution - 1 -

[0206] The calculation method of the inhibition rate of the sample on the formation of fluorescent AGEs is as follows:

[0207]

[0208] Each group is measured 3 times on average, and the average value is taken to calculate the inhibition rate value of AGEs generation. The results are shown in Table 9.

[0209] Table 9

[0210]

[0211]

[0212] From the data in Table 9, it can be seen that the composition described in this application has a good inhibitory effect on AGEs. The preparation methods of raspberry extract and rose extract will also affect the effect of the composition, and raspberry extract, guava extract, rose extract, and licorice extract have a certain synergistic effect in the above - mentioned efficacy, cooperate with each other in the above - mentioned efficacy, and are indispensable.

[0213] Test Example 7

[0214] Relative expression test of zebrafish collagen

[0215] The dermal connective tissue of the skin consists of cellular components and the extracellular matrix (ECM). Collagen and elastin account for 70% and 2-5% of the dry matter of the skin respectively, and are important components in the ECM of organisms. After being secreted by fibroblasts, collagen cross-links into a fibrous network structure of collagen fibers, which is the main compressive substance of the skin and can maintain the toughness of the skin. Type I collagen is a triple-helical molecule composed of two α1 chains and one α2 chain, which are encoded by the col1a1a and col1a1b genes respectively. The zebrafish genes col1a1a and col1a1b are homologous to the human type I collagen gene COL1A1. The relative expression levels of the skin type I collagen (col1a1a, col1a1b) genes are detected to evaluate whether the sample has anti-aging efficacy.

[0216] Wild-type zebrafish of the AB strain were used in the experiment. The zebrafish rearing method was carried out according to "The zebrafish book". The rearing water temperature was maintained at about 28.5°C, with 14 hours of light and 10 hours of darkness per day, and feeding was carried out once in the morning and once in the evening. One female fish and two male fish were placed in the spawning tank the night before collecting the embryos, and they were separated by a partition and placed in a dark environment. The next morning, after the light was turned on, the partition was removed, and the embryos were collected after spawning was completed. The embryos were placed in egg water and cultured in a light incubator at 28.5°C. Randomly selected 4dpf wild-type AB strain zebrafish were placed in a 6-well plate, with 30 zebrafish in each well (experimental group). The carnosine positive drug group (PC, concentration 100 μg / mL) and the sample solutions of Example 1 (C1), Example 10 (C2), and Comparative Example 4 (C3) (sample test concentration 35 μg / mL) were respectively given, and at the same time, a normal control group (Control) was set up. The volume of each well was 3 mL, and three parallel experiments were set up. After treatment at 28°C for 24 hours, zebrafish samples were collected according to the instructions of the collagen COL I ELISA kit, and data were collected using a multifunctional microplate reader to analyze the relative expression levels of the genes col1a1a and col1a1b in the zebrafish body. The results are as Figure 1 shown, from Figure 1 the results, it can be seen that the composition described in the present application can significantly increase the relative expression levels of the genes col1a1a and col1a1b in the zebrafish body.

[0217] Test Example 8

[0218] Test for melanin inhibition effect

[0219] Wild-type zebrafish of the AB strain were used in the experiment. The zebrafish were raised according to "The zebrafish book". The water temperature for raising was maintained at about 28.5°C, with 14 hours of light and 10 hours of darkness per day, and feeding was carried out once in the morning and once in the evening. One female fish and two male fish were placed in the spawning tank the night before collecting the embryos, and they were separated by a partition and placed in a dark environment. The next morning, after the light was turned on, the partition was removed, and the embryos were collected after spawning was completed. The embryos were placed in egg water and cultured in a light incubator at 28.5°C. On the day of collecting the embryos, at about 8 hpf, the liquid was completely changed to wash the embryos, and the embryos in poor condition were removed, and they were transferred to a 6-well plate, with 20 embryos placed in each well. The phenylthiourea positive drug group (PC, concentration 0.2 mM) and the sample solutions of Example 1 (C1), Example 10 (C2), and Comparative Example 4 (C3) (sample test concentration 35 μg / mL) were respectively given by water solution, and a normal control group (Control) was set up; when cultured in a light incubator at 28.5°C until 54 hpf, the state of the zebrafish embryos and the change of melanin were observed, and photos were taken with a stereomicroscope. The results of the effect of the composition on zebrafish melanin synthesis are as Figure 2 and Figure 3 shown. As can be seen from the figure, the composition described in the present application can significantly reduce zebrafish melanin synthesis.

[0220] The applicant declares that the present invention uses the above embodiments to illustrate a medicated and edible homologous composition with anti-aging and whitening effects and its application, but the present invention is not limited to the above embodiments, that is, it does not mean that the present invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, the equivalent replacement of each raw material of the product of the present invention, the addition of auxiliary components, the selection of specific methods, etc., all fall within the protection scope and the disclosure scope of the present invention.

[0221] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0222] In addition, it should be noted that, in the case of no contradiction, the various specific technical features described in the above specific embodiments can be combined in any suitable manner. In order to avoid unnecessary repetition, the present invention will not separately describe various possible combination methods.

Claims

1. A medicine-food homologous composition with anti-aging and whitening effects, characterized in that, The composition comprises 10 - 30 parts by mass of raspberry extract, 5 - 10 parts by mass of guava extract, 10 - 30 parts by mass of rose extract, and 1 - 5 parts by mass of licorice extract; The raspberry extract is prepared by a method comprising the following steps: mixing raspberry with water, performing ultrasonic extraction, filtering the extract through a filter bag and a ceramic membrane, concentrating, and drying to obtain the raspberry extract; The guava extract is prepared by a method comprising the following steps: mixing guava with water, performing reflux extraction, filtering through a filter bag and a ceramic membrane, concentrating, and drying to obtain the guava extract; The rose extract is prepared by a method comprising the following steps: mixing rose with water, performing negative pressure cavitation extraction, filtering through a filter bag and a ceramic membrane, concentrating, and drying to obtain the rose extract; Before the ultrasonic extraction, the raspberry - water mixture is further treated at 110 - 130 °C and 0.15 - 0.25 MPa for 5 - 10 min; The ratio of rose to water is 1 g:(40 - 60) mL, the pressure of the negative pressure cavitation extraction is 0.03 - 0.08 Mpa, the temperature is 50 - 60 °C, and the time is 1 - 1.5 h; The pore size of the ceramic membrane used in the raspberry extraction is 300 - 400 nm.

2. The homologous medicine and food composition with anti-aging and whitening effects according to claim 1, characterized in that, The power of the ultrasonic wave is 200 - 500 W, the frequency is 30 - 50 kHz, the temperature is 60 - 70 °C, and the time is 30 - 40 min.

3. The homologous composition of medicine and food with anti-aging and whitening effects according to claim 1, characterized in that, The ratio of guava to water is 1 g:(10 - 15) mL, and the reflux extraction time is 1 - 2 h.

4. Use of the homologous medicine - food composition with anti - aging and skin - whitening effects according to any one of claims 1 - 3 in the preparation of a product with skin - whitening, anti - aging or anti - glycation effects.

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