Growth retardant for garden plants and preparation method thereof

Abstract

The invention discloses a growth retardant for garden plants and a preparation method thereof, and belongs to the technical field of plant growth regulators. The liquid growth retardant for plants is prepared from the following raw materials: composite plant hormones, coumarin, organic acid, a tea residue extract, trace elements and water, the solid agent is a microbial agent. The mode of applying the microbial agent to the soil and spraying the liquid conditioning agent to the leaf surfaces is designed, so that the lawn quality is improved, the growth of the lawn is effectively delayed, the leaves are shortened, the grass layer density and the chlorophyll content are increased, the color is deepened, the lawn ornamental value is improved, and the landscape effect of lawn grass is prolonged.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators, and in particular relates to a growth retardant for garden plants and a preparation method thereof. Background Art

[0002] The quality of the lawn is closely related to the maintenance cost of the lawn. The higher the quality of the lawn, the higher the maintenance cost. The increase in lawn maintenance cost and the decrease in benefits have become a serious problem faced by lawn maintenance managers. How to alleviate the cost pressure is to use plant growth regulators in the daily maintenance of the lawn to promote lawn rooting, inhibit the upright growth of the lawn, and improve the resistance of lawn grass. In lawn maintenance, the reasonable application of plant growth regulators can effectively reduce energy consumption and mechanical wear, save labor resources, and improve lawn quality.

[0003] In lawn maintenance, plant growth retardants are often applied to inhibit the growth of lawn grass. Plant growth retardants inhibit the biosynthesis of gibberellins in plants. When the biosynthesis of gibberellins is inhibited, cell elongation stops, internodes become shorter, leaves become dark green, and the growth of the entire plant slows down. Plant growth retardants can slow down cell division and elongation of stem tissue, causing lawn plants to show physiological dwarfing; instead of causing the formation effect like auxin substances. In addition, although they can cause the entire plant to shrink, they will not completely prevent the growth of the plant, and the development progress and vitality of the plant are not affected by them, meeting the renewal needs of lawn grass.

[0004] Lawn plant growth regulators have become an effective technical measure in lawn management. Reasonable application of lawn plant growth regulators can effectively inhibit the growth of the aboveground part of the lawn, control heading, enhance the stress resistance and quality of the lawn, and effectively reduce the cost of lawn mowing and maintenance. However, due to the high requirements for application technology (such as application time, concentration, application method and standard) and the risk of toxic side effects (such as inhibiting the growth of lawn grass roots, reducing disease resistance, causing yellowing of leaves and lawns, etc.) of pure chemical regulators, the application of plant growth regulators on lawns is still in its infancy.

[0005] For example, Chinese patent application number CN97100966.X discloses a lawn growth regulator. Its purpose is to regulate the nutrition and metabolic rate of lawn grass, and the regulator uses paclobutrazol as a lawn grass growth regulator. However, this single use has large side effects and a narrow range of action. The lawn grass is dwarfed and the leaves lose green. At the same time, under high temperature and high humidity in summer, serious grass root rot and wilt diseases occur.

[0006] Therefore, how to use growth regulators to control the growth of lawn grass while preventing or reducing the possible adverse consequences thereof is a technical problem that needs to be solved urgently. Summary of the invention

[0007] In view of the problems existing in the prior art, the present invention provides a growth retardant for garden lawns. On the one hand, the vertical growth of lawns is effectively controlled to achieve the purpose of pruning, thereby reducing lawn maintenance costs; on the other hand, a certain positive effect is exerted on the color, tillering and root system of lawn grass; the stress resistance of lawn grass is improved, its adaptability to adversity is enhanced, and the quality of lawn is improved.

[0008] In order to achieve the above technical objectives, the technical solution adopted by the present invention is: A growth retardant for garden plants comprises a solid agent and a liquid agent, wherein the liquid agent comprises the following raw materials: compound plant hormone, coumarin, organic acid, tea residue extract, trace elements and water; the solid agent is a microbial agent.

[0009] Furthermore, the composite plant hormone is obtained by mixing paclobutrazol, chlormequat chloride and gibberellin in a mass ratio of 10:(5-8):(0.1-0.3).

[0010] Furthermore, the organic acid is jasmonic acid and wood vinegar, which are mixed in a volume ratio of 1:1.

[0011] Furthermore, the solid dosage form is a preparation method of a microbial agent, which is as follows: using corn as a host, inoculating the spores of Extreme Dependence and Abnormal Dependence into the root system of corn seedlings, planting them in a river sand zeolite culture matrix with a volume ratio of river sand: zeolite = 3:1, sterilizing them under high pressure steam at 121°C for 1h, and inoculating one Extreme Dependence ( Pillar preamplifier ) and a Pseudomonas anomala ( Caucasian pilara ) spores, and 3 corns are planted in each river sand zeolite culture matrix; after culturing under light for 12-16 weeks, mycelium of cyst mold appears in the culture matrix, and 100 mL of Hoagland's nutrient solution with a mass concentration of 25-35% is poured every 10-15 days during the culture period; the stems of the corn seedlings obtained after culturing for 12-16 weeks are removed, and the roots are retained, and the mycelium obtained in the culture matrix, the culture matrix and the retained corn roots are mixed and air-dried to obtain a microbial agent.

[0012] The spore amount in the obtained microbial inoculant is 70-100 spores / g.

[0013] Furthermore, the strain number of the extremely high Desmodium is CGMCC No.3.6638, purchased from the General Microbiology Center of the China National Microbiological Culture Collection Administration, and the original preservation time is September 1, 2003; the strain number of the abnormal Desmodium is CGMCC No.3.6654, purchased from the General Microbiology Center of the China National Microbiological Culture Collection Administration, and the original preservation time is September 1, 2003. Both strains can be purchased through the collection center without repeated biological preservation.

[0014] Furthermore, the preparation method of the tea residue extract is as follows: after crushing the tea residue, add water to grind it into a homogenate, adjust the pH to 6.5-7.5, add amylase and cellulase, and perform enzymolysis at 30-35°C for 12-18h to obtain an enzymolysis mixture; weigh 30g of the tea residue enzymolysis mixture and place it in a 500mL container, add 240mL of 80% ethanol solution, reflux extract twice at 80°C, extract for 1.5h, filter, and concentrate the filtrate under reduced pressure to obtain a crude tea extract, which is the tea residue extract.

[0015] Furthermore, the amount of amylase added is 0.3-0.5% of the mass of the tea residue, and the amount of cellulase added is 0.5-1% of the mass of the tea residue.

[0016] Furthermore, the trace elements are one or more of zinc sulfate, boric acid, molybdic acid, manganese sulfate, and copper sulfate.

[0017] A method for preparing a growth retardant for garden plants, characterized in that it comprises the following steps: (1) preparing solid microbial inoculants; (2) preparing a liquid agent: dispersing the compound plant hormone, coumarin, organic acid, tea residue extract, and trace elements in water respectively, and controlling the mass volume concentration of each component to be 300-500 mg / L of the compound plant hormone, 25-35 mg / L of coumarin, 15-25 mg / L of organic acid, 300-400 mg / L of tea residue extract, and 0.1-0.3 mg / L of trace elements; (3) The solid microbial agent and the liquid agent can be packaged separately and stored.

[0018] The growth retardant of the invention is mainly used for controlling lawn growth and improving its quality.

[0019] The method of using the growth retardant for garden plants of the present invention is as follows: the dosage of the solid agent is 50-100 g / m 2 , apply to the soil before sowing; 30 days after emergence, mow to a 3cm stubble height, and 2-3 days after mowing, spray the lawn grass with a liquid agent at a rate of 500mL / m 2 .

[0020] Beneficial effects: (1) First, the present invention uses Desmodium hypertrophum and Desmodium abnormalum to infect the root system of corn to prepare a solid microbial agent. Desmodium hypertrophum and Desmodium abnormalum are used together. During the growth process, they can effectively infect the plant root system and form arbuscular mycorrhiza with fungi, which can improve the growth conditions of lawn grass, including improving fertilizer utilization efficiency and root system depth, thereby accelerating the establishment speed of lawn and improving lawn quality. At the same time, the use and infection of the two fungi can also improve crop resistance. The fungi can also improve soil structure and promote the flow of air and water in the soil by forming a symbiotic relationship with the plant root system, thereby improving the drought resistance of the lawn. (2) Secondly, liquid microbial agents are used for treatment during the growth process, in which coumarin is added. The use of a small amount of coumarin can affect the balance of plant hormones and inhibit the plants. It is an effective plant growth retardant. Jasmonic acid and wood vinegar are added. Wood vinegar contains a variety of organic acids, phenols and alcohols. These ingredients help improve soil quality, increase soil fertility, and promote the reproduction of beneficial microorganisms. In addition, wood vinegar also has the function of inhibiting weed growth and preventing and controlling pests and diseases, thereby indirectly improving the overall health of the lawn. (3) Adding tea residue extract at an appropriate concentration, the tea alkaloids in the tea can inhibit the synthesis of gibberellins in plants and delay the growth of meristems without affecting the quality of the lawn. It can increase the lawn density, improve the leaf color and structure, and improve the visual quality of the lawn. (4) Manganese participates in photosynthesis and is a cofactor for many enzymes, which is essential for lignin biosynthesis. Boron exists in the cell wall and requires the structural integrity of the cell wall. Adding trace elements can promote the metabolism of lawn cells, improve photosynthesis efficiency, improve leaf quality, and improve the overall quality of the lawn; (5) In summary, the present invention is designed to apply microbial agents to the soil and spray liquid regulators on the leaves. While improving the quality of the lawn, it effectively delays the growth of the grass, shortens and coarsens the leaves, increases the density of the grass layer and the chlorophyll content, and deepens the color, thereby improving the ornamental value of the lawn and prolonging the landscape effect of the lawn grass. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a diagram showing the root infection effects of the experimental groups of Example 1 of the present invention and Comparative Example 1 and Comparative Example 2; Figure 2 The effect of different treatment groups on the plant height of Poa annua; Figure 3 This is the effect of different treatment groups on the plant height of tall fescue. DETAILED DESCRIPTION

[0022] The technical solution of the present invention is further described below in conjunction with specific embodiments, but is not limited thereto.

[0023] Example 1 A growth retardant for garden plants comprises a solid agent and a liquid agent, wherein the liquid agent comprises the following raw materials: compound plant hormone, coumarin, organic acid, tea residue extract, trace elements and water; the solid agent is a microbial agent.

[0024] The composite plant hormone is obtained by mixing paclobutrazol, chlormequat chloride and gibberellin in a mass ratio of 10:5:0.3.

[0025] The organic acid is jasmonic acid and wood vinegar, which are mixed in a volume ratio of 1:1.

[0026] The solid dosage form is a microbial agent. The preparation method is as follows: using corn as a host, inoculating the spores of Extreme Dependence and Abnormal Dependence into the root system of corn seedlings, planting them in a river sand zeolite culture matrix with a volume ratio of river sand to zeolite of 3:1, sterilizing them under high pressure steam at 121°C for 1h, and inoculating one Extreme Dependence ( Preamplifier ) and a Pseudomonas anomala ( Caucasian pilara ) spores, 3 corns are planted in each river sand zeolite culture matrix; after 12 weeks of light culture, cysticercus hyphae appear in the culture matrix, and 100 mL of Hoagland's nutrient solution with a mass concentration of 25% is poured every 15 days during the culture period; the corn seedlings obtained after 12 weeks of culture are removed from the stems, and the roots are retained, and the hyphae obtained in the culture matrix, the culture matrix and the retained corn roots are mixed and air-dried to obtain a microbial agent. The spore amount in the obtained microbial agent is 70-80 spores / g.

[0027] The strain number of the extremely high Desmodium is CGMCC No.3.6638, purchased from the General Microbiology Center of the China Microorganism Culture Collection Administration Committee, and the original preservation time is September 1, 2003; the strain number of the abnormal Desmodium is CGMCC No.3.6654, purchased from the General Microbiology Center of the China Microorganism Culture Collection Administration Committee, and the original preservation time is September 1, 2003. Both strains can be purchased through the collection center without repeated biological preservation.

[0028] The preparation method of the tea residue extract is as follows: after crushing the tea residue, adding water to grind it into a homogenate, adjusting the pH to 6.5-7.5, adding amylase and cellulase, and performing enzymolysis at 30-35° C. for 12 hours to obtain an enzymolysis mixture; weighing 30 g of the tea residue enzymolysis mixture and placing it in a 500 mL container, adding 240 mL of 80% ethanol solution, reflux extracting twice at 80° C. for 1.5 hours, filtering, and concentrating the filtrate under reduced pressure to obtain a crude tea extract, namely the tea residue extract.

[0029] The amount of amylase added is 0.3% of the mass of the tea residue, and the amount of cellulase added is 0.5% of the mass of the tea residue.

[0030] The trace elements are zinc sulfate, boric acid, molybdic acid, manganese sulfate and copper sulfate mixed in a mass ratio of 1:2:3:1:0.5.

[0031] A method for preparing a growth retardant for garden plants, characterized in that it comprises the following steps: (1) preparing solid microbial inoculants; (2) preparing a liquid agent: dispersing the compound plant hormone, coumarin, organic acid, tea residue extract, and trace elements in water respectively, and controlling the mass volume concentration of each component to be 300 mg / L of the compound plant hormone, 25 mg / L of coumarin, 15 mg / L of organic acid, 300 mg / L of tea residue extract, and 0.1 mg / L of trace elements; (3) The solid microbial agent and the liquid agent can be packaged separately and stored.

[0032] Example 2 A growth retardant for garden plants comprises a solid agent and a liquid agent, wherein the liquid agent comprises the following raw materials: compound plant hormone, coumarin, organic acid, tea residue extract, trace elements and water; the solid agent is a microbial agent.

[0033] The composite plant hormone is obtained by mixing paclobutrazol, chlormequat chloride and gibberellin in a mass ratio of 10:7:0.1.

[0034] The organic acid is jasmonic acid and wood vinegar, which are mixed in a volume ratio of 1:1.

[0035] The solid dosage form is a microbial agent. The preparation method is as follows: using corn as a host, inoculating the spores of Extreme Dependence and Abnormal Dependence into the root system of corn seedlings, planting them in a river sand zeolite culture matrix with a volume ratio of river sand to zeolite of 3:1, sterilizing them under high pressure steam at 121°C for 1h, and inoculating one Extreme Dependence ( Preamplifier ) and a Pseudomonas anomala ( Caucasian pilara ) spores, 3 corns are planted in each river sand zeolite culture matrix; after 14 weeks of light culture, cysticercus hyphae appear in the culture matrix, and 100 mL of Hoagland's nutrient solution with a mass concentration of 30% is poured every 13 days during the culture period; the corn seedlings obtained after 14 weeks of culture are removed from the stems, and the roots are retained, and the hyphae obtained in the culture matrix, the culture matrix and the retained corn roots are mixed and air-dried to obtain a microbial agent. The spore amount in the obtained microbial agent is 80-90 spores / g.

[0036] The strain number of the extremely high Desmodium is CGMCC No.3.6638, purchased from the General Microbiology Center of the China Microorganism Culture Collection Administration Committee, and the original preservation time is September 1, 2003; the strain number of the abnormal Desmodium is CGMCC No.3.6654, purchased from the General Microbiology Center of the China Microorganism Culture Collection Administration Committee, and the original preservation time is September 1, 2003. Both strains can be purchased through the collection center without repeated biological preservation.

[0037] The preparation method of the tea residue extract is as follows: after crushing the tea residue, adding water to grind it into a homogenate, adjusting the pH to 6.5-7.5, adding amylase and cellulase, and performing enzymolysis at 30-35° C. for 15 hours to obtain an enzymolysis mixture; weighing 30 g of the tea residue enzymolysis mixture and placing it in a 500 mL container, adding 240 mL of 80% ethanol solution, reflux extracting twice at 80° C. for 1.5 hours, filtering, and concentrating the filtrate under reduced pressure to obtain a crude tea extract, namely the tea residue extract.

[0038] The amount of amylase added is 0.4% of the mass of the tea residue, and the amount of cellulase added is 0.7% of the mass of the tea residue.

[0039] The trace elements are obtained by mixing zinc sulfate, boric acid, molybdic acid, manganese sulfate, copper sulfate and the like.

[0040] A method for preparing a growth retardant for garden plants, characterized in that it comprises the following steps: (1) preparing solid microbial inoculants; (2) preparing a liquid agent: dispersing the compound plant hormone, coumarin, organic acid, tea residue extract, and trace elements in water respectively, and controlling the mass volume concentration of each component to be 400 mg / L of compound plant hormone, 30 mg / L of coumarin, 20 mg / L of organic acid, 350 mg / L of tea residue extract, and 0.2 mg / L of trace elements; (3) The solid microbial agent and the liquid agent can be packaged separately and stored.

[0041] Example 3 A growth retardant for garden plants comprises a solid agent and a liquid agent, wherein the liquid agent comprises the following raw materials: compound plant hormone, coumarin, organic acid, tea residue extract, trace elements and water; the solid agent is a microbial agent.

[0042] The composite plant hormone is obtained by mixing paclobutrazol, chlormequat chloride and gibberellin in a mass ratio of 10:8:0.3.

[0043] The organic acid is jasmonic acid and wood vinegar, which are mixed in a volume ratio of 1:1.

[0044] The solid dosage form is a microbial agent. The preparation method is as follows: using corn as a host, inoculating the spores of Extreme Dependence and Abnormal Dependence into the root system of corn seedlings, planting them in a river sand zeolite culture matrix with a volume ratio of river sand to zeolite of 3:1, sterilizing them under high pressure steam at 121°C for 1h, and inoculating one Extreme Dependence ( Preamplifier ) and a Pseudomonas anomala ( Caucasian pilara ) spores, 3 corns are planted in each river sand zeolite culture matrix; light culture is carried out for 16 weeks, and cyst mold hyphae appear in the culture matrix, and 100 mL of Hoagland's nutrient solution with a mass concentration of 35% is poured every 15 days during the culture period; the corn seedlings obtained after 16 weeks of culture are removed from the stems, and the roots are retained, and the hyphae obtained in the culture matrix, the culture matrix and the retained corn roots are mixed and air-dried to obtain a microbial agent. The spore amount in the obtained microbial agent is 90-100 spores / g.

[0045] The strain number of the extremely high Desmodium is CGMCC No.3.6638, purchased from the General Microbiology Center of the China Microorganism Culture Collection Administration Committee, and the original preservation time is September 1, 2003; the strain number of the abnormal Desmodium is CGMCC No.3.6654, purchased from the General Microbiology Center of the China Microorganism Culture Collection Administration Committee, and the original preservation time is September 1, 2003. Both strains can be purchased through the collection center without repeated biological preservation.

[0046] The preparation method of the tea residue extract is as follows: after crushing the tea residue, adding water to grind it into a homogenate, adjusting the pH to 6.5-7.5, adding amylase and cellulase, and performing enzymolysis at 30-35° C. for 18 hours to obtain an enzymolysis mixture; weighing 30 g of the tea residue enzymolysis mixture and placing it in a 500 mL container, adding 240 mL of 80% ethanol solution, refluxing and extracting it twice at 80° C. for 1.5 hours, filtering, and concentrating the filtrate under reduced pressure to obtain a crude tea extract, namely the tea residue extract.

[0047] The amount of amylase added is 0.5% of the mass of the tea residue, and the amount of cellulase added is 1% of the mass of the tea residue.

[0048] The trace elements are zinc sulfate, boric acid, molybdic acid, manganese sulfate and copper sulfate mixed in a mass ratio of 1:1:2:1:0.8.

[0049] A method for preparing a growth retardant for garden plants, characterized in that it comprises the following steps: (1) preparing solid microbial inoculants; (2) preparing a liquid agent: dispersing the compound plant hormone, coumarin, organic acid, tea residue extract, and trace elements in water respectively, and controlling the mass volume concentration of each component to be 500 mg / L of compound plant hormone, 35 mg / L of coumarin, 25 mg / L of organic acid, 400 mg / L of tea residue extract, and 0.3 mg / L of trace elements; (3) The solid microbial agent and the liquid agent can be packaged separately and stored.

[0050] Comparative Example 1 A growth retardant for garden plants comprises a solid agent and a liquid agent, wherein the liquid agent comprises the following raw materials: compound plant hormone, coumarin, organic acid, tea residue extract, trace elements and water; the solid agent is a microbial agent.

[0051] The solid dosage form is a microbial agent. The preparation method is as follows: using corn as a host, inoculating the spores of Extreme Dependence on the root system of corn seedlings, planting them in a river sand zeolite culture matrix with a volume ratio of river sand to zeolite of 3:1, sterilizing them under high pressure steam at 121°C for 1 hour, and inoculating one Extreme Dependence on the root system of one corn plant ( Preamplifier ) spores, 3 corns are planted in each river sand zeolite culture matrix; after 12 weeks of light culture, cysticercus hyphae appear in the culture matrix, and 100 mL of Hoagland's nutrient solution with a mass concentration of 25% is poured every 15 days during the culture period; the corn seedlings obtained after 12 weeks of culture are removed from the stems, and the roots are retained, and the hyphae obtained in the culture matrix, the culture matrix and the retained corn roots are mixed and air-dried to obtain a microbial agent. The spore amount in the obtained microbial agent is 70-80 spores / g.

[0052] In this comparative example, except that the solid dosage form only contains Denomyces exigua, the other raw materials and preparation method are the same as those of Example 1.

[0053] Comparative Example 2 A growth retardant for garden plants comprises a solid agent and a liquid agent, wherein the liquid agent comprises the following raw materials: compound plant hormone, coumarin, organic acid, tea residue extract, trace elements and water; the solid agent is a microbial agent.

[0054] The solid dosage form is a microbial agent. The preparation method is as follows: using corn as a host, inoculating the spores of Abnormal Desmodium into the root system of corn seedlings, planting them in a river sand zeolite culture matrix with a volume ratio of river sand to zeolite of 3:1, sterilizing them under high pressure steam at 121°C for 1 hour, and inoculating one Abnormal Desmodium ( Caucasian pilara) spores, 3 corns are planted in each river sand zeolite culture matrix; after 12 weeks of light culture, cysticercus hyphae appear in the culture matrix, and 100 mL of Hoagland's nutrient solution with a mass concentration of 25% is poured every 15 days during the culture period; the corn seedlings obtained after 12 weeks of culture are removed from the stems, and the roots are retained, and the hyphae obtained in the culture matrix, the culture matrix and the retained corn roots are mixed and air-dried to obtain a microbial agent. The spore amount in the obtained microbial agent is 70-80 spores / g.

[0055] In this comparative example, except that only E. anomala is included in the solid dosage form, the rest of the raw materials and the preparation method are the same as those in Example 1.

[0056] Comparative Example 3 A growth retardant for garden plants comprises a solid agent and a liquid agent, wherein the liquid agent comprises the following raw materials: compound plant hormone, coumarin, organic acid, trace elements and water; and the solid agent is a microbial agent.

[0057] A method for preparing a growth retardant for garden plants, characterized in that it comprises the following steps: (1) preparing solid microbial inoculants; (2) preparing a liquid agent: dispersing the composite plant hormone, coumarin, organic acid, and trace element in water respectively, and controlling the mass volume concentration of each component to be 300 mg / L of composite plant hormone, 25 mg / L of coumarin, 15 mg / L of organic acid, and 0.1 mg / L of trace element; (3) The solid microbial agent and the liquid agent can be packaged separately and stored.

[0058] In this comparative example, except that the tea residue extract was not added to the liquid agent, the other raw materials and preparation method were the same as those in Example 1.

[0059] Comparative Example 4 A growth retardant for garden plants comprises a solid agent and a liquid agent, wherein the liquid agent comprises the following raw materials: compound plant hormone, coumarin, organic acid, tea residue extract, trace elements and water; the solid agent is a microbial agent.

[0060] A method for preparing a growth retardant for garden plants, characterized in that it comprises the following steps: (1) preparing solid microbial inoculants; (2) Preparation of liquid agent: Disperse the compound plant hormone, coumarin, organic acid, tea residue extract, and trace elements in water respectively, and control the mass volume concentration of each component to be 300 mg / L of compound plant hormone, 25 mg / L of coumarin, 15 mg / L of organic acid, 200 mg / L of tea residue extract, and 0.1 mg / L of trace elements respectively; (3) The solid microbial agent and the liquid agent can be packaged separately and stored.

[0061] In this comparative example, except that the concentration of the tea residue extract was reduced to 200 mg / L, the other raw materials and preparation method were the same as those in Example 1.

[0062] Comparative Example 5 A growth retardant for garden plants comprises a solid agent and a liquid agent, wherein the liquid agent comprises the following raw materials: compound plant hormone, coumarin, organic acid, tea residue extract, trace elements and water; the solid agent is a microbial agent.

[0063] A method for preparing a growth retardant for garden plants, characterized in that it comprises the following steps: (1) preparing solid microbial inoculants; (2) Preparation of liquid agent: Disperse the compound plant hormone, coumarin, organic acid, tea residue extract, and trace elements in water respectively, and control the mass volume concentration of each component to be 300 mg / L of compound plant hormone, 25 mg / L of coumarin, 15 mg / L of organic acid, 500 mg / L of tea residue extract, and 0.1 mg / L of trace elements respectively; (3) The solid microbial agent and the liquid agent can be packaged separately and stored.

[0064] In this comparative example, except that the concentration of the tea residue extract was increased to 500 mg / L, the other raw materials and preparation method were the same as those in Example 1.

[0065] Experimental testing Pot experiment: The test material was a cool-season turfgrass species, ryegrass (Lolium perenne L.).

[0066] Test matrix: The soil used was ordinary farmland soil in Linyi City, Shandong Province, taken from the nursery of East District of Linyi University. The soil was sieved through a 2 mm soil sieve and then mixed with vermiculite and perlite in a ratio of 5:3:2 to form the test matrix.

[0067] Experimental methods: The sieved soil was mixed with vermiculite and perlite at a ratio of 5:3:2, and the mixed soil matrix was sterilized at 121°C in a high-temperature sterilizer to complete the preparation of the matrix. The size of the flower pot used for the planting pot: length × width × height is 13 cm × 9 cm × 11 cm respectively. The planting pots used were sprayed with 5% potassium permanganate solution for disinfection and then exposed to sunlight for 8 hours, and then the matrix was divided into portions, and the matrix was divided into portions per pot. The sowing amount was 50 grains / pot, and the solid bacterial agent of the embodiment was applied to the soil before sowing. After 30 days of emergence, the stubble height was trimmed to 3 cm. After 2-3 days of trimming, the grass was sprayed with a liquid agent for foliar spraying.

[0068] The experiment set up 11 treatment groups, each group was repeated five times, and the experimental results were averaged, which are: S1: using the plant growth retardant of Example 1; S2: using the plant growth retardant of Example 2; S3: using the plant growth retardant of Example 3; S4: using the plant growth retardant of Comparative Example 1; S5: using the plant growth retardant of Comparative Example 2; S6: using the plant growth retardant of Comparative Example 3; S7: using the plant growth retardant of Comparative Example 4; S8: using the plant growth retardant of Comparative Example 5; S9: Paclobutrazol control: Paclobutrazol (PB): purchased from Zhengzhou Nongda Biochemical Products Co., Ltd., 15% wettable powder. Used after diluting 1200 times according to the instructions; S10: Chlormequat control: Chlormequat (CCC): purchased from Anyang Quanfeng Biotechnology Co., Ltd., 50% chlormequat aqueous solution, diluted 300 times according to the instructions for use; S11: CK and equal amount of clean water.

[0069] Index determination: 14 days after the spraying, fresh plant leaves were collected to measure the plant height, chlorophyll content, leaf area and other indicators of perennial ryegrass. The root activity and leaf proline content of perennial ryegrass were measured. The plant height was measured by the natural height method, and 10 ryegrasses were selected for each treatment. The chlorophyll content was measured by the acetone ethanol method, the proline content was measured by the sulfosalicylic acid-acid ninhydrin method, and the root activity was measured by the TTC method. The leaf area was scanned by an instrument, and 10 leaves with uniform growth were randomly selected for each treatment, scanned with an HP.C7717 Singaporean instrument, and analyzed by the Delta-TSCAN analysis system.

[0070] Table 1 Test results

[0071] It can be seen from the table data that the application of the plant growth regulator of the embodiment of the present invention can effectively reduce plant height and leaf area, while maintaining high leaf chlorophyll content and proline content, and high leaf quality. However, comparative examples 1 and 2, which changed the composition of the solid bacterial agent, had little effect on indicators such as plant height and leaf area, but had a certain degree of effect on leaf quality. It can be seen that the composition of the strain is more critical to the improvement of leaf surface quality. The infection ability of a single strain and the effect of improving the quality of the leaves are lower than those of two strains acting simultaneously, and the two show a synergistic effect.

[0072] Comparative Example 3 which lacks tea residue extract and Comparative Examples 5-9 which have changed the concentration of tea residue extract, both showed varying degrees of decline in the delaying effect on plants and the improvement effect on leaf quality. This shows that tea residue extract can delay crop growth to a certain extent and improve crop quality and resistance. However, if the concentration is too low, the delaying effect is not obvious, and if the concentration is too high, it will affect the leaf quality. Therefore, appropriate concentration is the key to achieving its regulatory effect.

[0073] The mycorrhizal infection rates of Example 1, Comparative Example 1, Comparative Example 2, and blank group CK were further tested. The harvested plant roots were rinsed with distilled water, and then soaked in 30% alcohol. The roots were then cut into small sections of about 1 cm, placed in a glass test tube, and immersed in 10% KOH. After heating in a water bath, the roots were acidified with HCl, and then stained with trypan blue. Finally, the infection effect was observed under a microscope after decolorization. The infection effect was as follows: Figure 1 As shown in the figure, it can be seen that the CK root system has no infection and discoloration phenomenon. Only a small part of the root tip of Comparative Example 1 and Comparative Example 2 has obvious blue staining, while Example 1 has a significant amplification of the blue staining phenomenon. Under the treatment effect of Example 1, the infection condition is obviously the best, the infection rate is the highest, and the leaf quality is higher.

[0074] Amplification experiment: The experiment was conducted in a greenhouse at the experimental base of the Academy of Agricultural Sciences in Linyi, Shandong Province. The grass species were Yum Bluegrass and Qiancao No. 1 tall fescue. The seeds were sown by broadcasting on May 10, 2022, and observations and measurements were carried out on October 15, 2022.

[0075] The experimental samples are: the growth retardant of Example 1-3, the dosage of the solid agent is 50g / m 2 , apply to the soil before sowing; 30 days after emergence, mow to a 3cm stubble height, and 2-3 days after mowing, spray the lawn grass with a liquid agent at a rate of 500mL / m 2 The use method of comparative examples 1-5 is the same as that of the embodiment. The dilution multiple of 50% chlormequat aqueous solution (produced by Sichuan Guoguang Industrial Co., Ltd.) is 300 times; the dilution multiple of 15% paclobutrazol wettable powder (produced by Jiangsu Jianpai Pesticide Chemical Co., Ltd.) is 1200 times. After 30 days of emergence, the stubble height is 3 cm. After 2-3 days of pruning, the lawn grass is sprayed on the leaves. The dosage is 500g / m 2 The blank control was sprayed with an equal amount of water.

[0076] There were 2 grass seeds, 10 pesticides, 1 blank control, 3 repetitions, a total of 2×11×3=66 plots, the plot area was 1.0 m×0.5 m, and the dry matrix of nutrient soil in each plot was 2.0 kg. The sowing rates of Baisheng Bluegrass and Qiancao No. 1 Tall Fescue were 20 and 35 g / m respectively. 2 .

[0077] Observation items and methods: The quality of lawn can be evaluated and measured by an evaluation system consisting of some internal characteristics or external features that represent the growth quality of lawn grass. At present, the common lawn quality evaluation methods and systems are relatively complex. Different regions and different planting users have different standards and scales for evaluating lawn quality. In the actual production process, there is still no unified and recognized lawn quality evaluation method. According to the purpose of lawn construction and use, the commonly used lawn evaluation standards can be divided into lawn appearance quality, use quality, ecological quality and lawn base quality.

[0078] Landscape evaluation indicators such as density, coverage, uniformity, and color are selected to form the evaluation system. In order to objectively evaluate the quality of lawns, especially the landscape quality of lawns, the lawn comprehensive evaluation index grading method is adopted for scoring evaluation. Each landscape indicator is scored 0-5 points, and the scoring method includes the quantitative grades of some indicators (Table 2).

[0079] (1) Density: Randomly select sampling points in the plot and place a 20 cm long ruler at the sampling location. Count the number of lawn grass branches per 20 cm along the long side of the ruler, and then calculate the number of branches per unit length. Count three times at random points in each plot (a total of 60 cm) and then take the average value, expressed as plants / cm. The data records are found in Table 2 to obtain the score corresponding to the density.

[0080] (2) Coverage: The percentage of the area covered by the lawn grass is used as the evaluation standard. The specific evaluation method and score are shown in Table 2. (3) Uniformity: The uniformity of lawn appearance is evaluated using the subjective perception method. The observer makes a comprehensive evaluation of the lawn based on his or her subjective perception of the uniformity of the lawn's overall appearance, flatness, lawn density, texture, and color difference.

[0081] (4) Color: The color of healthy turfgrass under normal irrigation management conditions is the highest standard, and the score is given on a 5-point scale based on visual inspection results; the lower the score, the worse the quality. The value assignment method is shown in Table 2. The values ​​in the table are the average of the readings of three replicate plots of the same treatment in each test.

[0082] Table 2 Grading standards for comprehensive evaluation indicators of lawns

[0083] Table 3. Grain bluegrass quality index test scoring results

[0084] Table 4 Tall Fescue quality index test scoring results

[0085] It should be noted that the above embodiments are only partial embodiments of the preferred methods of implementing the present invention, rather than all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

Claims

1. A growth retardant for garden plants, characterized in that: It comprises a solid agent and a liquid agent, wherein the liquid agent comprises the following raw materials: compound plant hormones, coumarin, organic acid, tea residue extract, trace elements and water; the solid agent is a microbial agent.

2. The growth retardant for garden plants according to claim 1, characterized in that: The composite plant hormone is obtained by mixing paclobutrazol, chlormequat chloride and gibberellin in a mass ratio of 10:(5-8):(0.1-0.3).

3. The growth retardant for garden plants according to claim 1, characterized in that: The organic acid is jasmonic acid and wood vinegar, which are mixed in a volume ratio of 1:

1.

4. The growth retardant for garden plants according to claim 1, characterized in that: The preparation method of the solid dosage microbial agent comprises the following steps: using corn as a host, inoculating spores of Demastica maxima and Demastica anomala into the root system of corn seedlings, and planting the corn seedlings in a river sand zeolite culture matrix with a volume ratio of river sand: zeolite=3:1 and sterilized under high pressure steam at 121°C for 1h, inoculating one spore of Demastica maxima and one spore of Demastica anomala into one corn root system, and planting three corns in each river sand zeolite culture matrix; culturing under light for 12-16 weeks, until cyst mycelia appear in the culture matrix, and pouring 100 mL of Hoagland's nutrient solution with a mass concentration of 25-35% every 10-15 days during the culturing period; removing the stems of the corn seedlings obtained after culturing for 12-16 weeks, retaining the root system, and mixing the mycelia obtained in the culture matrix, the culture matrix and the retained corn root system, and air-drying the mixture to obtain the microbial agent.

5. The growth retardant for garden plants according to claim 4, characterized in that: The strain number of the extremely high Denomyces is CGMCC No. 3.6638, which was purchased from the General Microbiology Center of the China National Microbiological Culture Collection Administration; the strain number of the abnormal Denomyces is CGMCC No. 3.6654, which was purchased from the General Microbiology Center of the China National Microbiological Culture Collection Administration.

6. The growth retardant for garden plants according to claim 1, characterized in that: The preparation method of the tea residue extract is as follows: after crushing the tea residue, adding water to grind it into a homogenate, adjusting the pH to 6.5-7.5, adding amylase and cellulase, and performing enzymolysis at 30-35° C. for 12-18 hours to obtain an enzymolysis mixture; weighing 30 g of the tea residue enzymolysis mixture and placing it in a 500 mL container, adding 240 mL of 80% ethanol solution, reflux extracting twice at 80° C. for 1.5 hours, filtering, and concentrating the filtrate under reduced pressure to obtain a crude tea extract, namely the tea residue extract.

7. The growth retardant for garden plants according to claim 6, characterized in that: The amount of amylase added is 0.3-0.5% of the mass of the tea residue, and the amount of cellulase added is 0.5-1% of the mass of the tea residue.

8. The growth retardant for garden plants according to claim 1, characterized in that: The trace elements are one or more of zinc sulfate, boric acid, molybdic acid, manganese sulfate and copper sulfate.

9. A method for preparing the growth retardant for garden plants according to any one of claims 1 to 8, characterized in that: The following steps are involved: (1) preparing solid microbial inoculants; (2) preparing a liquid agent: dispersing the composite plant hormone, coumarin, organic acid, tea residue extract, and trace elements in water respectively, and controlling the mass volume concentrations of the composite plant hormone, coumarin, organic acid, tea residue extract, and trace elements to be 300-500 mg / L for the composite plant hormone, 25-35 mg / L for coumarin, 15-25 mg / L for the organic acid, 300-400 mg / L for the tea residue extract, and 0.1-0.3 mg / L for the trace elements; (3) The solid microbial agent and the liquid agent can be packaged separately and stored.

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