Method for improving quality of feed triticale silage

Abstract

The invention is suitable for the technical field of livestock feed, and relates to a method for improving the quality of feed triticale silage, which comprises the following steps: (1) mixing: uniformly mixing triticale with a compound bacteria solution with the concentration of 1-4% to obtain a mixture, wherein the compound bacteria consist of feruloyl esterase-producing lactobacillus plantarum with the living cell number range of 106-108 CFU / g and bacillus velezensis with the living cell number range of 106-108 CFU / g; and (2) anaerobic fermentation: performing anaerobic fermentation on the mixture in the step (1) under the conditions that the temperature is 25-30 DEG C and the relative humidity is 40-50% for 60 days to obtain the triticale silage. The method for improving the silage quality of the feed triticale has the beneficial effects that the degradation of wood fibers is improved, the content of soluble carbohydrates and lactic acid is increased, the content of ammoniacal nitrogen is reduced, and harmful bacteria are inhibited, so that the silage fermentation quality of the triticale is improved.

Description

Technical Field

[0001] The invention relates to the technical field of livestock feed, and more particularly to a method for improving the quality of triticale silage for feeding. Background Art

[0002] There are many ways to process forage, the two most common methods are hay making and silage. However, hay making usually relies on drying, which easily leads to the loss of nutrients in the forage. Given the abundant rainfall in the southern region, making hay is difficult, so silage technology has broad application prospects in the region. However, the silage fermentation process involves biochemical reactions such as plant respiration, plant microbial protein hydrolysis, clostridial fermentation, and amino acid decarboxylation, which may have adverse effects on the nutrients of the feed, such as increasing the loss of energy and nutrients, resulting in a decrease in dry matter (DM) and nutritional value in silage. This not only affects the utilization value of the feed, but also may reduce the profit of the dairy production system. As a grass forage, forage triticale also faces the problem of nutrient loss during the silage preparation process. Due to its high fiber content, these components may affect the activity of microorganisms during the fermentation process, thereby affecting the quality of silage and feeding effect. Therefore, how to reduce the nutrient loss of forage triticale during the silage process and improve its silage quality has become a challenge that needs to be solved urgently.

[0003] At present, the research focus of forage triticale in China is mainly on variety selection and nutrient analysis. In recent years, there have been relatively few studies on forage triticale silage, which mainly focus on improving the silage quality of forage triticale through additives. However, the research on the use of microbial additives with fiber degradation ability to regulate the silage quality of forage triticale is still quite limited, and its regulation mechanism is rarely reported. Summary of the invention

[0004] In view of the shortcomings of the prior art, the object of the present invention is to provide a method for improving the silage quality of triticale for feeding, which improves the degradation of wood fiber, while increasing the content of soluble carbohydrates and lactic acid, reducing the content of ammonia nitrogen, and inhibiting harmful bacteria, thereby improving the silage fermentation quality of triticale.

[0005] To achieve the above object, the present invention provides the following technical solution: a method for improving the quality of triticale silage for feeding, comprising the following steps: (1) Mixing: Mix the triticale with a composite bacterial solution having a concentration of 1% to 4% to obtain a mixture, wherein the composite bacteria has a viable cell count ranging from 10 6 ~10 8 CFU / g of ferulic acid esterase-producing Lactobacillus plantarum and the number of viable cells ranged from 10 6 ~108 Composition of Bacillus velez CFU / g; (2) Anaerobic fermentation: The mixture in step (1) is subjected to anaerobically fermentation for 60 days at a temperature of 25 to 30° C. and a relative humidity of 40% to 50% to obtain triticale silage.

[0006] Furthermore, in step (1), the composite bacteria has a viable cell number ranging from 10 6 ~10 8 CFU / g of ferulic acid esterase-producing Lactobacillus plantarum and the number of viable cells ranged from 10 6 ~10 8 CFU / g composition of Aspergillus niger.

[0007] Furthermore, in step (1), the mass ratio of the triticale to the composite bacterial liquid is 1:0.01 to 1:0.02.

[0008] Furthermore, the triticale is harvested at the milky stage and crushed into particles with a length of 1 to 2 cm before mixing in step (1), and the moisture content is controlled at 70%.

[0009] Among them, the source of ferulic acid esterase-producing Lactobacillus plantarum was purchased from China General Microbiological Culture Collection Center with the accession number CGMCC12955; the source of Bacillus velezensis was purchased from Beijing Institute of Biological Studies with the accession number BNCC341566; the source of Aspergillus niger was purchased from Beijing Institute of Biological Studies with the accession number BNCC186380.

[0010] The advantages of the present invention are as follows: the present invention discloses a method for improving the quality of silage of triticale for feeding, wherein in the process of silage of triticale, a composite bacteria of Bacillus velez and Lactobacillus plantarum producing ferulic acid esterase, and a composite bacteria of Lactobacillus plantarum producing ferulic acid esterase and Aspergillus niger are added respectively, which can effectively inhibit the degradation of protein, improve the degradation of wood fiber, and at the same time increase the content of soluble carbohydrates and lactic acid, reduce the content of ammonia nitrogen, inhibit harmful bacteria, thereby improving the silage fermentation quality of triticale. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 This is a distribution diagram of the bacterial community structure of forage rye based on the phylum classification level of the present invention.

[0012] Figure 2 This is a distribution diagram of the bacterial community structure of forage rye based on the genus classification level of the present invention. DETAILED DESCRIPTION

[0013] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application may be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0014] It should be noted that, unless otherwise specified, all technical and scientific terms used in this application have the same meanings as commonly understood by ordinary technicians in the technical field to which this application belongs.

[0015] In the present invention, unless otherwise specified, the directions used, such as "up" and "down", usually refer to the directions shown in the drawings, or to the vertical, perpendicular or gravity directions; similarly, for ease of understanding and description, "left" and "right" usually refer to the left and right shown in the drawings; "inside" and "outside" refer to the inside and outside relative to the outline of each component itself, but the above-mentioned directions are not used to limit the present invention.

[0016] Example 1: A method for improving the quality of triticale silage for forage, comprising the following steps: (1) Mixing: 500 g of triticale was mixed evenly with 5 mL of a composite bacterial solution with a concentration of 1% to 4% to obtain a mixture, wherein the composite bacteria consisted of a viable cell count ranging from 10 6 ~10 8 CFU / g of ferulic acid esterase-producing Lactobacillus plantarum and the number of viable cells ranged from 10 6 ~10 8 CFU / g of Bacillus Velez; the triticale is harvested at the milky stage and crushed into particles with a length of 1 to 2 cm before mixing, and the moisture content is controlled at 70%.

[0017] (2) Anaerobic fermentation: The mixture in step (1) is subjected to anaerobically fermentation for 60 days at a temperature of 25 to 30° C. and a relative humidity of 40% to 50% to obtain triticale silage.

[0018] Example 2: A method for improving the quality of triticale silage for forage, comprising the following steps: (1) Mixing: 500 g of triticale was mixed evenly with 5 mL of a composite bacterial solution with a concentration of 1% to 4% to obtain a mixture, wherein the composite bacteria consisted of a viable cell count ranging from 10 6 ~10 8 CFU / g of ferulic acid esterase-producing Lactobacillus plantarum and the number of viable cells ranged from 10 6 ~10 8 CFU / g of Aspergillus niger; the triticale is harvested at the milky stage and crushed into particles with a length of 1 to 2 cm before mixing, and the moisture content is controlled at 70%.

[0019] (2) Anaerobic fermentation: The mixture in step (1) is placed under conditions of a temperature of 25 to 30° C. and a relative humidity of 40% to 50% for 60 days to obtain triticale silage.

[0020] Comparative Example 1: The overall method is the same as that of Example 1, except that no composite bacterial solution is added and 5 mL of pure water is added.

[0021] Comparative Example 2: The overall method is the same as that of Example 1, except that the composite bacteria only consist of 10 living cells. 6 ~10 8 CFU / g composition of Aspergillus niger.

[0022] Comparative Example 3: The overall method is the same as that of Example 1, except that the composite bacteria only consist of 10 living cells. 6 ~10 8 CFU / g of ferulic acid esterase-producing Lactobacillus plantarum.

[0023] Comparative Example 4: The overall method is the same as that of Example 1, except that the composite bacteria only consist of 10 living cells. 6 ~10 8 CFU / g composition of Bacillus Velezii.

[0024] Experiment 1: Effects of different microbial additives and combinations on the nutritional composition of triticale silage for feeding The triticale silage obtained in Examples 1-2 and Comparative Examples 1-4 was dried in an oven at 65°C, the DM content was determined, and then crushed and sieved through a 1 mm sieve for analysis of crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and water-soluble carbohydrates (WSC). The CP content was determined using a Kjeldahl nitrogen analyzer, and the NDF and ADF were determined using an Ankom 2000 fiber analyzer. The WSC content was determined using an anthrone-sulfuric acid colorimetric method, and the results are shown in Table 1.

[0025] Table 1 Effects of different microbial additives and combinations on the nutritional components of triticale silage for feeding

[0026] Note: Different capital letters AE indicate significant differences between different anaerobic fermentation days in the same treatment ( P <0.05); different lowercase letters in ad represent the same anaerobic fermentation There were significant differences among the treatments in terms of days ( P <0.05). T: treatment; D: days of anaerobic fermentation; T×D: interaction between treatment and days of anaerobic fermentation.

[0027] After 3 days of anaerobic fermentation, the DM content of the comparative example 2 treatment was higher than that of the comparative example 1 treatment, increased by 0.43%, and the ADF content was lower than that of the comparative example 1 treatment, decreased by 2.82%; the DM and CP contents of the comparative example 3 treatment were higher than those of the comparative example 1 treatment, increased by 0.68% and 0.80%, and the ADF content was lower than that of the comparative example 1 treatment, decreased by 3.18%; the DM, CP and WSC contents of the comparative example 4 treatment were higher than those of the comparative example 1 treatment, increased by 0.46%, 0.57% and 0.28%, and the ADF content was lower than that of the comparative example 1 treatment, decreased by 3.05%; the CP and WSC contents of the embodiment 2 treatment were higher than those of the comparative example 1 treatment, increased by 1.97% and 0.67%, and the NDF and ADF contents were lower than those of the comparative example 1 treatment, decreased by 4.77% and 5.93%; the CP and WSC contents of the embodiment 1 treatment were higher than those of the comparative example 1 treatment, increased by 2.12% and 0.68%, and the NDF and ADF contents were lower than those of the comparative example 1 treatment, decreased by 4.75% and 6.07% ( P <0.05). After 60 days of anaerobic fermentation, the DM content of the comparative example 2 treatment was higher than that of the comparative example 1 treatment, and increased by 0.43%, and the ADF content was lower than that of the comparative example 1 treatment, and decreased by 2.82%; the DM and CP contents of the comparative example 3 treatment were higher than those of the comparative example 1 treatment, and increased by 0.68% and 0.40%, and the ADF content was lower than that of the comparative example 1 treatment, and decreased by 3.18%; the DM, CP and WSC contents of the comparative example 4 treatment were higher than those of the comparative example 1 treatment, and increased by 0.46%, 0.57% and 0.28%, and the ADF content was lower than that of the comparative example 1 treatment, and decreased by 3.05%; the CP and WSC contents of the example 2 treatment were higher than those of the comparative example 1 treatment, and increased by 1.97% and 0.67%, and the NDF and ADF contents were lower than those of the comparative example 1 treatment, and decreased by 9.59% and 5.93%. The CP and WSC contents of the example 1 treatment were higher than those of the comparative example 1 treatment, and increased by 2.12% and 0.68%, and the NDF and ADF contents were lower than those of the comparative example 1 treatment, and decreased by 10.05% and 6.07% ( P <0.05). After 60 days of anaerobic fermentation, the CP and WSC contents of the samples treated in Example 2 and Example 1 were the highest, and the NDF and ADF contents of the samples treated in Example 2 and Example 1 were the lowest.

[0028] Because Bacillus Velez is a facultative anaerobic bacterium, it can consume oxygen, create an anaerobic environment, stimulate the growth of lactic acid bacteria and the formation of an acidic environment for silage, thereby inhibiting the growth of corrupt microorganisms and minimizing nutrient loss. At the same time, Aspergillus niger can secrete a variety of enzymes such as cellulase, which can promote fiber degradation, thereby increasing the soluble substrate that can be utilized for silage, thereby reducing the consumption of CP content in silage by microorganisms. In addition, the inoculated ferulic acid esterase-producing plant lactobacillus itself has a certain inhibitory effect on other bacteria with the ability to degrade CP, so that the CP content after treatment in Example 1 and Example 2 is the highest. Bacillus Velez produces cellulase during silage fermentation, thereby accelerating the degradation of NDF and ADF. And Aspergillus niger can secrete a variety of enzymes such as cellulase, which can promote fiber degradation. Since the ferulic acid produced by Lactobacillus plantarum producing ferulic acid esterase can also degrade fiber, the degradation degree of NDF and ADF can be increased by inoculating Lactobacillus plantarum and Bacillus Velez together in Example 1, and by inoculating Lactobacillus plantarum and Aspergillus niger together in Example 2.

[0029] Experiment 2: Effects of different microbial additives and combinations on the fermentation characteristics of triticale silage Weigh 20 g of the triticale silage obtained in Examples 1-2 and Comparative Examples 1-4, add 180 mL of sterile water, crush and mix evenly in a laboratory blender for 1 min, and then filter through 4 layers of 7.5×7.5 cm sterile cheesecloth. Use the filtered filtrate to determine the pH value, ammonia nitrogen (NH3-N) and organic acid. The filtrate was centrifuged at 10,000 rpm, 10 min, and 4°C. The pH was determined using a pH meter, and the contents of lactic acid (LA), acetic acid (AA), propionic acid (PA), and butyric acid (BA) were determined using high performance liquid chromatography. The NH3-N content was determined using the phenol-sodium hypochlorite colorimetric method, and the results are shown in Table 2.

[0030] Table 2 Effects of different microbial additives and combinations on the fermentation characteristics of triticale silage

[0031] Note: Different capital letters AE indicate significant differences between different anaerobic fermentation days in the same treatment ( P <0.05); different lowercase letters in ad indicate significant differences among different treatments at the same anaerobic fermentation days ( P <0.05). T: treatment; D: days of anaerobic fermentation; T×D: interaction between treatment and days of anaerobic fermentation.

[0032] After 3 days of anaerobic fermentation, the pH values ​​of all additive treatment groups were lower than those of the comparative example 1 treatment group, and the LA content was higher than that of the comparative example 1 treatment group. The lowest pH values ​​of Example 2 and Example 1 were 4.07 and 4.03, and the highest pH value of the comparative example 1 treatment was 5.86. The highest LA contents of Example 2 and Example 1 treatments were 2.72% and 2.84%, and the lowest LA content of the comparative example 1 treatment was 0.97%; the AA content of comparative example 4 was higher than that of the comparative example 1 treatment group, and the highest AA content of the comparative example 4 treatment was 0.45%; the NH3-N content of the comparative example 3, Example 2 and Example 1 treatments was lower than that of the comparative example 1 treatment group, and the lowest NH3-N content of the comparative example 2 and Example 1 treatments was 0.33% and 0.31%, and the highest NH3-N content of the comparative example 1 treatment was 1.42%. After 7 days of anaerobic fermentation, the pH values ​​of all additive treatment groups were lower than those of the comparative example 1 treatment group, and the LA content was higher than that of the comparative example 1 treatment group. The lowest pH value of the treatment in Example 2 was 3.85, and the highest pH value of the treatment in Comparative Example 1 was 5.07. The highest LA content of the treatment in Example 1 was 3.59%, and the lowest LA content of the treatment in Comparative Example 1 was 1.25%. The NH3-N content of all additive treatments was lower than that of the comparative example 1 treatment group. The lowest NH3-N content of the treatment in Example 2 was 0.51%, and the highest NH3-N content of the treatment in Comparative Example 1 was 2.38%. After 15 days of anaerobic fermentation, the pH values ​​of all additive treatment groups were lower than those of the comparative example 1 treatment group, and the LA content was higher than that of the comparative example 1 treatment group. The lowest pH value of the treatment in Example 2 was 3.86, and the highest pH value of the treatment in Comparative Example 1 was 4.91. The highest LA content of the treatment in Example 1 was 3.93%, and the lowest LA content of the treatment in Comparative Example 1 was 1.36%; the AA content of the treatments in Example 2 and Example 1 was higher than that in Comparative Example 1, the highest AA content of the treatment in Example 2 was 0.64%, and the lowest AA content of the treatment in Comparative Example 1 was 0.29%; the NH3-N content of all additive treatments was lower than that of the comparative example 1 treatment group, the lowest NH3-N content of the treatment in Example 1 was 0.62%, and the highest NH3-N content of the treatment in Comparative Example 1 was 2.81%. After 30 days of anaerobic fermentation, the pH values ​​of all additive treatment groups were lower than those of the comparative example 1 treatment group, and the LA content was higher than that of the comparative example 1 treatment group. The lowest pH value of the treatment in Example 1 was 3.78, and the highest pH value of the treatment in Comparative Example 1 was 4.70. The highest LA content of the treatment in Example 1 was 4.12%, and the lowest LA content of the treatment in Comparative Example 1 was 1.62%; the AA content of all additive treatment groups was higher than that of the comparative example 1 treatment group, and the NH3-N content was lower than that of the comparative example 1 treatment group. The highest AA content of the treatment in Example 2 was 2.06%, and the lowest AA content of the treatment in Comparative Example 1 was 0.45, the lowest NH3-N content of the treatment in Example 1 was 0.76%, and the highest NH3-N content of the treatment in Comparative Example 1 was 3.53%.After 60 days of anaerobic fermentation, the pH values ​​of all additive treatment groups were lower than those of the comparative example 1 treatment group, and the LA content was higher than that of the comparative example 1 treatment group. The lowest pH value of the treatment of Example 1 was 3.73, and the highest pH value of the treatment of Comparative Example 1 was 4.66. The highest LA content of the treatment of Example 1 was 4.63%, and the lowest LA content of the treatment of Comparative Example 1 was 2.00%. The NH3-N content of all additive treatments was lower than that of Comparative Example 1. The lowest NH3-N content of Example 1 was 1.01%, and the highest NH3-N content of Comparative Example 1 was 3.90%. In addition, the BA content was detected only in the comparative example 1 treatment, and the BA content was not detected in other treatments.

[0033] Experiment 3: Effects of different microbial additives and combinations on bacterial community structure after 60 days of anaerobic fermentation of triticale at the phylum and genus level The total genomic DNA of fresh triticale samples, examples 1 and 2, and triticale silage after anaerobic fermentation for 60 days in comparative examples 1-4 was extracted by CTAB method. The purity and concentration of DNA were determined by agarose gel electrophoresis, and the purified DNA was diluted with sterile water to a concentration of 1 ng / μL. The diluted DNA was used as a template and polymerase chain reaction was performed using GC Buffer. The PCR amplification products were detected by 2% agarose gel electrophoresis. The PCR products mixed in equal proportions were mixed on a 2% agarose gel and then electrophoresed. The end sequences of the amplified DNA fragments were connected using DNA adhesion enzymes, and the DNA fragments were purified and screened by AMpure PB pure magnetic beads. The SMRT Bell library was constructed, and the library was quantified by Qubit concentrators and sequenced on the PacBio Sequel platform. The full-length sequence of the 16S ribosomal RNA (RRNA) gene was amplified using specific primers of (1514R and 27F) barcodes. The amplicon library was sequenced using the Illumina MiSeq platform. The principal coordinate analysis, alpha diversity analysis, and canonical correlation analysis of the microbial community were performed using the NovoMagic platform developed by Beijing Xinyuan Biotechnology Co., Ltd. The OTUS, Shannon, and ACE indices were calculated using QIIME software (version 1.9.1). The results are shown in Figure 1 and Figure 2 .

[0034] like Figure 1 As shown, at the phylum level, Firmicutes was the main dominant phylum in the treatments of Comparative Example 2, Comparative Example 3, Comparative Example 4, Example 2 and Example 1, with relative abundances of 80.04%, 84.45%, 81.55%, 89.07% and 90.60%, respectively. Proteobacteria was the main dominant phylum in the fresh sample of triticale and the treatment of Comparative Example 1, with relative abundances of 88.11% and 50.80%, respectively.

[0035] like Figure 2 As shown, at the genus level, Enterococcus genus ( Enterococcus ) was the dominant genus with a relative abundance of 20.00%, including Lactobacillus ( Lactiplantibacillus )、Delftella Delftia ), Weissella ( Weissella ), Klebsiella spp. Klebsiella ), Ralstonia ( Ralstonia )、Stereotrophomonas ( Stenotrophomonas ), Leuconostoc spp. Leuconostoc ) and Cossaccharomyces spp. ( Kosakonia ). In the comparative example 1 Lactiplantibacillus and Weissella The relative abundances of the dominant bacterial genus were 18.38% and 17.67%. Lactiplantibacillus is the dominant genus, with a relative abundance of 77.30%, including Delftia , Weissella , Enterococcu s, Klebsiella , Ralstonia , Stenotrophomonas , Leuconostoc and Kosakonia , the relative abundances were 0.27%, 8.30%, 6.36%, 2.31%, 0.47%, 0.15%, 0.68% and 0.35% respectively. Lactiplantibacillus is the dominant genus, with a relative abundance of 81.75%, including Delftia , Weissella , Enterococcus , Klebsiella , Ralstonia , Stenotrophomonas and Leuconostoc , the relative abundances were 0.59%, 0.09%, 0.97%, 2.77%, 3.13%, 2.18% and 0.15% respectively. Lactiplantibacillus is the dominant genus, with a relative abundance of 78.14%, including Delftia , Weissella , Enterococcus , Klebsiella , Ralstonia , Stenotrophomonas , Leuconostoc and Kosakonia , the relative abundances were 2.00%, 5.38%, 5.65%, 0.80%, 0.50%, 0.44%, 3.45% and 0.12% respectively. Lactiplantibacillus and DelftiaThe dominant bacterial genus, with relative abundances of 40.85% and 48.44%, also contains Weissella , Ralstonia , Stenotrophomonas and Leuconostoc , the relative abundances were 0.06%, 5.04%, 0.68% and 0.09% respectively. Lactiplantibacillus and Delftia The dominant bacterial genus, with relative abundances of 43.57% and 50.15%, also contains Weissella , Ralstonia , Stenotrophomonas and Kosakonia , with relative abundances of 0.03%, 3.75%, 0.63% and 0.03% respectively.

[0036] Delftia The genus is a non-fermentative chemical organic nutrient bacteria, usually found in soil, water and plants. It can degrade phenolic compounds and aniline in polluted soil and water. Lactiplantibacillus The relative abundance of is lower than that of the comparative examples 2, 3 and 4, but the fermentation quality is better than that of the comparative examples 2, 3 and 4. The reason for this result is that Delftia Compared with the treatments in Comparative Examples 2, 3 and 4, the lower pH values ​​in the treatments in Examples 1 and 2 are consistent with the Delftia The relative abundance of , and the lower pH environment is conducive to the preservation of silage nutrition and the improvement of silage quality. Delftia The relative abundance was high, indicating that the synergistic effects of Bacillus velez and feruloyl esterase-producing Lactobacillus plantarum, as well as Aspergillus niger and feruloyl esterase-producing Lactobacillus plantarum, favored Delftia Growth. Adding Bacillus Velezii + Lactobacillus plantarum producing ferulic acid esterase, and adding Aspergillus niger + Lactobacillus plantarum producing ferulic acid esterase can promote the growth of beneficial bacteria ( Lactiplantibacillus and Delftia ) growth, thereby inhibiting harmful bacteria ( Weissella , Enterococcus , Kosakonia ) and thus preserve the nutritional quality of fodder rye.

[0037] The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. A method for improving the quality of triticale silage for forage, characterized in that: It includes the following steps: (1) Mixing: Mix the triticale with a composite bacterial solution having a concentration of 1% to 4% to obtain a mixture, wherein the composite bacteria has a viable cell count ranging from 10 6 ~10 8 CFU / g of ferulic acid esterase-producing Lactobacillus plantarum and the number of viable cells ranged from 10 6 ~10 8 CFU / g of Velez bacteria; or the composite bacteria consist of viable cells ranging from 10 6 ~10 8 CFU / g of ferulic acid esterase-producing Lactobacillus plantarum and the number of viable cells ranged from 10 6 ~10 8 The composition of Aspergillus niger in CFU / g; (2) Anaerobic fermentation: The mixture in step (1) is subjected to anaerobically fermentation for 60 days at a temperature of 25 to 30° C. and a relative humidity of 40% to 50% to obtain triticale silage.

2. A method for improving the quality of triticale silage for forage according to claim 1, characterized in that: In step (1), the mass ratio of the triticale to the composite bacterial liquid is 1:0.01 to 1:0.

02.

3. A method for improving the quality of triticale silage according to claim 2, characterized in that: The triticale is harvested at the milky stage and crushed into particles with a length of 1 to 2 cm before mixing in step (1), and the moisture content is controlled at 70%.

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