High-flux phytoplankton cell counting pretreatment equipment

Abstract

The invention discloses high-flux phytoplankton cell counting pretreatment equipment, and belongs to the technical field of biological / plant detection sampling, the high-flux phytoplankton cell counting pretreatment equipment comprises a first base body used for placing a container, a second base body arranged on the side of the first base body, a moving module capable of moving relative to the container is arranged on the second base body, and a first pipe body is connected to the moving module; the moving module is connected with a peristaltic pump through a second pipe body, and the first pipe body communicates with the second pipe body. According to the invention, comprehensive automatic sample pretreatment is realized, manual work is completely replaced, the treatment progress and speed are fast, the equipment maintenance is simple, and the operation stability is high.

Description

Technical Field

[0001] The invention belongs to the technical field of biological / plant detection sampling, and in particular relates to a high-throughput phytoplankton cell counting pre-processing device. Background Art

[0002] The identification and counting of phytoplankton in water bodies is of great significance to the analysis, evaluation and protection of water environment. Phytoplankton usually refers to floating algae, including cyanobacteria, green algae, diatoms, golden algae, etc. At present, microscopic observation of phytoplankton samples is an important means for algae researchers and monitors to identify and count algae. The density of algae per unit volume is calculated by magnifying and counting with a microscope. It is used for ecological evaluation of water bodies. In the process of sample preparation and algae identification and counting, a lot of time and energy of algae researchers are required, which reduces the efficiency of algae detection and its popularization and promotion capabilities. Therefore, as an important part of the automatic monitoring method of phytoplankton, it is of great significance to realize the automatic sampling of phytoplankton samples.

[0003] The prior art provides many solutions to solve the problems of operation time and complicated procedures for researchers. For example, the existing Korean patent KR101698000B1 discloses a multi-purpose sample injection device, the module of which is provided as a replaceable syringe, which can realize automatic sampler solid phase extraction and solid phase micro-extraction. The device can be used to measure algal toxins and related substances optionally online in real time. However, the structure of the device is relatively complex, and there is room for improvement in equipment operation and maintenance. Summary of the invention

[0004] The purpose of the present invention is to provide a high-throughput phytoplankton cell counting pretreatment device to achieve fully automated sample pretreatment, completely replace manual work, and have fast processing progress and speed, simple equipment maintenance, and high operating stability.

[0005] In order to solve the above technical problems, the present invention specifically provides the following technical solutions: a high-throughput phytoplankton cell counting pretreatment device, which includes a first substrate for placing a container, a second substrate is provided on the side of the first substrate, and a movable module that can be displaced relative to the container is provided on the second substrate. The movable module is connected to a first tube body, and the movable module is connected to a peristaltic pump through the second tube body. The first tube body and the second tube body are connected.

[0006] The present invention adopts a peristaltic pump in conjunction with a mobile module to achieve high-throughput fully automatic sampling and processing of samples inside a certain number of containers placed on the first substrate, and it is a continuous processing mode, which solves the problems of operating time and complex processes for researchers. Compared with the prior art, the present case adopts the first tube body and the second tube body to directly perform sampling and processing mode, which can avoid the problem of subsequent maintenance and troubleshooting difficulties caused by the complex design of the sampling equipment. In addition, the present invention only needs to perform sampling directly through the first tube body and the second tube body. Compared with the multi-tube connection scheme of the existing equipment, the suction force of the first tube body and the second tube body of this case has low internal loss, which can solve the problem of sample retention or adhesion inside the pipeline, and reduce the operation difficulty and cost for subsequent maintenance / repair of the equipment.

[0007] According to one embodiment of the present invention, the mobile module includes a first mobile module that can move in the horizontal direction, the first mobile module is arranged on the second base, the first mobile module is provided with a second mobile module that can move in the vertical direction, and the second mobile module is provided with a clamping assembly. The first module can reciprocate along the direction of the first base container array, so that the second mobile module can be driven to move to the top of the container at different positions, and the second mobile module works to make the first tube enter the container for sampling. The first mobile module uses a driving motor control method to control the displacement of the first mobile module, that is, the existing commonly used X-axis or Y-axis moving coordinate device. Similarly, the second mobile module also uses a driving motor control method to realize Z-axis lifting. This is the prior art and will not be overly repeated. It should be noted that by designing the first mobile module and the second mobile module to move with the clamping assembly, the upper part of the clamping assembly is used to clamp the first tube or the second tube so that it can be sampled in sequence, intelligently, and realize high-throughput fully automatic sampling and processing, and it is a continuous processing mode, which solves the problems of operating time and complex processes for researchers.

[0008] According to one embodiment of the present invention, the clamping assembly has a first clamping plate connected to the second moving module, and the upper and lower ends of the first clamping plate are respectively provided with a second clamping plate and a third clamping plate arranged horizontally, and the surfaces of the second clamping plate and the third clamping plate are provided with a third hole, and the third hole can allow the first tube body or the second tube body to enter and exit. The first clamping plate provided can make the clamping assembly fixedly connected to the second moving module, so that the operation of the second moving module can drive the clamping assembly to move, and the height of the inlet section of the first tube body above the container is calculated according to the parameters such as the length size of the first tube body or the second tube body clamped on the clamping assembly, and then the time for the first tube body to start the peristaltic pump in the process of entering the container is calculated, so as to achieve precise control, and the error rate is low compared with manual operation, and the second clamping plate and the third support plate of the clamping assembly can simultaneously clamp multiple first tube bodies and second tube bodies and guide the first tube body and the second tube body to align with the center of the container, so as to avoid the deviation of the first tube body or the second tube body from the center of the container to affect the suction effect of the sampling fluid.

[0009] According to one embodiment of the present invention, the second clamping plate and the third clamping plate are both provided with a first strip-shaped groove body, and the third through holes are both provided on the first groove body. One end of the first groove body is connected to the side of the second clamping plate and the third clamping plate, and the side of the second clamping plate and the third clamping plate is provided with a second hole body connected to the first groove body. The arrangement of the first transcendence and the third through hole can be applicable to the clamping and assembly of the first tube body and the second tube body in a wider size range. At the same time, for the first tube body or the second tube body with a smaller size, the internal tube body can be pressurized and limited by arranging fasteners or plungers in the second hole body. Through the above-mentioned method, while ensuring the clamping and limiting of the clamping plate and the first tube body or the second tube body, the deformation of the tube body at the clamping limit position is reduced or avoided. In addition, the gap in the first groove body provided in the clamping assembly during the movement with the moving module can provide a deformation buffering effect of the tube body in the third through hole, reduce or avoid the deformation of the tube body clamping position of the clamping plate during the movement, thereby ensuring that the flow rate of the sample on the entire moving trajectory during the suction process is relatively consistent, thereby ensuring the suction speed of the sample and avoiding problems such as suction blockage.

[0010] According to one embodiment of the present invention, the container includes a first container and a second container, and a first hole body capable of accommodating the first container is provided on the first substrate, and a circular groove having a diameter larger than that of the first hole body is provided on the hole wall of the first hole body. The first container is a cylindrical structure, and the second container is a box body or other shaped structures. The first hole body provided on the first substrate is used to place the first container in an array on the first substrate, so that the phytoplankton pretreatment operation can be carried out in batches in sequence, thereby improving work efficiency. A further solution of providing a circular groove on the first hole body is used to increase the adaptability range of the first hole body. In addition, for sample leakage or liquid exposure that may occur during the sampling process, the structural feature of the peak gap between the circular groove and the first container placed in the first hole body can be utilized to promote the collection of these leaked materials into the first hole body to avoid affecting the internal substances of other containers or subsequent operations.

[0011] According to one embodiment of the present invention, the peristaltic pump is arranged on a mobile frame, and a positioning block or a moving wheel is arranged at the bottom of the mobile frame. In this way, the number of peristaltic pumps can be increased or decreased or the model of peristaltic pumps can be changed according to the needs on the mobile frame, which is convenient for operation, and the mobile frame can be displaced or fixed according to the needs to reduce the difficulty of equipment assembly.

[0012] According to one embodiment of the present invention, the level of the inlet end of the peristaltic pump is higher than the level of the upper end of the container, thereby improving the sampling efficiency.

[0013] According to one embodiment of the present invention, the first base is horizontally arranged, the second base is vertically arranged on the side of the first base, and the movable module is arranged above the container on the first base.

[0014] The sample processing method using a high-throughput phytoplankton cell counting pre-processing device comprises the following steps: Step 1: Place the sample in a container, add the first test solution, and let it stand; Step 2: After the standing time is over, obtain the supernatant and bottom sediment in the container, remove the supernatant on the top of the container by suction, and use a peristaltic pump to suck the sediment in the container.

[0015] According to one embodiment of the present invention, the first test solution is Lugo test solution.

[0016] According to one embodiment of the present invention, after the moving module drives the first tube body to move to the target container, the peristaltic pump is started to extract the sediment through the first tube body and the second tube body; According to one embodiment of the present invention, the moving module drives the first tube body to move to the target container and then places the end of the first tube body into the container. The peristaltic pump is started during the downward movement of the first tube body relative to the container, so that the first tube body can be sucked while descending, thereby solving the problem of suction disturbance.

[0017] Compared with the prior art, the present invention has the following beneficial effects: the present invention adopts a peristaltic pump in conjunction with a mobile module to achieve high-throughput fully automatic sampling and processing of a certain number of samples inside containers placed on the first substrate, and it is a continuous processing mode, solving the problems of operating time and complex processes for researchers. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0019] Figure 1 This is a schematic diagram of the high-throughput phytoplankton cell counting pre-treatment equipment of the present invention; Figure 2 It is a front view of the high-throughput phytoplankton cell counting pre-processing device of the present invention; Figure 3 It is a schematic diagram of a connection scheme of the peristaltic pump and the first movable module and the second movable module of the present invention; Figure 4 It is a schematic diagram of a first hole body on a first substrate of the present invention; Figure 5 It is a schematic diagram of the structural scheme of the clamping assembly of the present invention; Figure 6 It is a schematic diagram of a connection scheme between a peristaltic pump and a mobile rack of the present invention; Figure 7It is a schematic diagram of the connection component scheme of the present invention.

[0020] Description of reference numerals: 10-first base; 11-first container; 12-second base; 13-first hole; 14-second container; 20-first movable module; 30-second movable module; 40-first tube; 50-second tube; 60-peristaltic pump; 70-clamping assembly; 71-first clamping plate; 72-second clamping plate; 73-third clamping plate; 74-second hole; 75-first trough; 76-third hole; 80-movable frame; 90-connecting assembly; 91-first plate; 92-connecting shaft; 93-second plate; 94-fourth hole. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] The following first describes the concepts involved in the present application in conjunction with the accompanying drawings. It should be noted that the following description of each concept is only to make the content of the present application easier to understand, and does not limit the scope of protection of the present application; at the same time, the embodiments and features in the embodiments of the present application can be combined with each other in the absence of conflict. The present application will be described in detail with reference to the accompanying drawings and in conjunction with the embodiments.

[0023] Embodiment 1 like Figure 1-Figure 6 As shown, a high-throughput phytoplankton cell counting pretreatment device, the high-throughput phytoplankton cell counting pretreatment device, the device includes a first substrate 10 for placing a container, a second substrate 12 is provided on the side of the first substrate 10, a movable module that can be displaced relative to the container is provided on the second substrate 12, a first tube body 40 is connected to the movable module, the movable module is connected to a peristaltic pump 60 through a second tube body 50, and the first tube body 40 and the second tube body 50 are connected.

[0024] The present invention adopts a peristaltic pump 60 in cooperation with a mobile module to realize high-throughput fully automatic sampling and processing of a certain number of internal samples of containers placed on the first substrate 10, and it is a continuous processing mode, which solves the problems of operation time and complex processes for researchers. Compared with the prior art, the present case adopts the first tube body 40 and the second tube body 50 to directly perform the sampling and processing mode, which can avoid the problem of subsequent maintenance and troubleshooting difficulties caused by the complex design of the sampling equipment. In addition, the present invention only needs to perform sampling directly through the first tube body 40 and the second tube body 50. Compared with the multi-tube connection scheme of the existing equipment, the suction force of the first tube body 40 and the second tube body 50 in this case has low internal loss, which can solve the problem of sample retention or adhesion inside the pipeline, and reduce the operation difficulty and cost for subsequent maintenance / repair of the equipment.

[0025] The movable module includes a first movable module 20 capable of moving in a horizontal direction. The first movable module 20 is disposed on the second base 12 . A second movable module 30 capable of moving in a vertical direction is disposed on the first movable module 20 . A clamping assembly 70 is disposed on the second movable module 30 . The first module 20 can reciprocate along the direction of the container array of the first substrate 10, so that the second movable module 30 can be driven to move to the top of the container at different positions. The second movable module 30 works to make the first tube 40 enter the container for sampling. The first movable module 20 adopts a driving motor control method to control the displacement of the first movable module 20, that is, the existing commonly used X-axis or Y-axis moving coordinate device. Similarly, the second movable module 30 also adopts a driving motor control method to achieve Z-axis lifting. This is the prior art and will not be described in detail. It should be noted that the first movable module 20 and the second movable module 30 are designed to move with the clamping assembly 70. The upper part of the clamping assembly 70 is used to clamp the first tube 40 or the second tube 50, so that sampling can be carried out in sequence and intelligently, realizing high-throughput fully automatic sampling processing, and it is a continuous processing mode, which solves the problems of operating time and complex processes for researchers.

[0026] The clamping assembly 70 has a first clamping plate 71 connected to the second movable module 30. The upper and lower ends of the first clamping plate 71 are respectively provided with a horizontally arranged second clamping plate 72 and a third clamping plate 73. The surfaces of the second clamping plate 72 and the third clamping plate 73 are both provided with a third hole 76, and the third hole 76 can allow the first tube body 40 or the second tube body 50 to enter and exit. The first clamping plate 71 is capable of fixing the clamping assembly 70 with the second movable module 30, so that the operation of the second movable module 30 can drive the clamping assembly 70 to move. The height of the inlet section of the first tube body 40 above the container is calculated based on parameters such as the length of the first tube body 40 or the second tube body 50 clamped on the clamping assembly 70, and then the time for starting the peristaltic pump 60 in the process of the first tube body 40 entering the container is calculated, thereby realizing precise control with a lower error rate than manual operation. In addition, the second clamping plate 72 and the third supporting plate 73 of the clamping assembly 70 can clamp multiple first tube bodies 40 and second tube bodies 50 at the same time and guide the first tube body 40 and the second tube body 50 to align with the center of the container, thereby preventing the first tube body 40 or the second tube body 50 from deviating from the center of the container and affecting the sampling fluid suction effect. For example, if the bottom port of the first tube body 40 is not located at the center of the container, and due to the direction of the inner wall of the container, the suction force at the port of the first tube body 40 may be greater near the inner wall of the container during the suction process, causing sample disturbance along the inner wall of the container for a greater distance downward instead of following the rule of sequential downward suction at the end of the first tube body 40. This may affect the accuracy of the final sample pre-processing data.

[0027] The second clamping plate 72 and the third clamping plate 73 are both provided with a first strip-shaped groove 75, and the third through hole 76 is provided on the first groove 75. One end of the first groove 75 is connected to the side of the second clamping plate 72 and the third clamping plate 73. The side of the second clamping plate 72 and the third clamping plate 73 is provided with a second hole 74 connected to the first groove 75. The arrangement of the first transcendence 75 and the third through hole 76 can be applicable to the clamping assembly of the first tube body 40 and the second tube body 50 of a wider range of sizes. At the same time, for the first tube body 40 or the second tube body 50 of a smaller size, a fastener or a column can be set in the second hole 74. The inner tube body is pressurized and limited by means of a plug, etc., so that the deformation of the tube body at the clamping limit position can be reduced or avoided while ensuring the clamping limit between the clamping plate and the first tube body 40 or the second tube body 50. In addition, when the clamping assembly 70 moves with the moving module, the gap in the first groove body 75 can provide a deformation buffering effect for the tube body in the third through hole 76, thereby reducing or avoiding the deformation of the clamping position of the tube body during the movement of the clamping plate, thereby ensuring that the flow rate of the sample on the entire moving trajectory during the suction process is relatively consistent, thereby ensuring the suction speed of the sample and avoiding problems such as suction blockage.

[0028] The container includes a first container 11 and a second container 14. A first hole 13 capable of accommodating the first container 11 is provided on the first substrate 10, and a circular groove having a diameter greater than that of the first hole 13 is provided on the hole wall of the first hole 13. The first container 11 is a cylindrical structure, and the second container 14 is a box body or other shaped structures. The first hole 13 provided on the first substrate 10 is used to place the first container 11 in an array on the first substrate 10, so that the phytoplankton pretreatment operation can be carried out in batches in sequence, thereby improving work efficiency. Further, a circular groove is provided on the first hole 13 to improve the adaptability range of the first hole 13. In addition, for the possible leakage of samples or liquid exposure during the sampling process, the structural feature of the peak gap between the circular groove and the first container 11 placed in the first hole 13 can be utilized to promote the collection of these leaked materials into the first hole 13 to avoid affecting the internal materials of other containers or subsequent operations.

[0029] The peristaltic pump 60 is arranged on the mobile frame 80, and a positioning block or a moving wheel is arranged at the bottom of the mobile frame 80. In this way, the number of peristaltic pumps can be increased or decreased or the model of peristaltic pumps can be changed on the mobile frame 80 according to the needs, which is convenient for operation, and the mobile frame 80 can be displaced or fixed according to the needs to reduce the difficulty of equipment assembly.

[0030] The horizontal height of the inlet end of the peristaltic pump 60 is higher than the horizontal height of the upper end of the container, thereby improving the sampling efficiency.

[0031] The first base 10 is horizontally arranged, the second base 12 is vertically arranged at the side of the first base 10 , and the moving module is arranged above the container on the first base 10 .

[0032] The sample processing method using a high-throughput phytoplankton cell counting pre-processing device comprises the following steps: Step 1: Place the sample in a container, add the first test solution, and let it stand; Step 2: After the standing time is over, obtain the supernatant and the bottom sediment in the container, remove the supernatant on the top of the container by suction, and use the peristaltic pump 60 to suck the sediment in the container.

[0033] According to one embodiment of the present invention, the first test solution is Lugo test solution.

[0034] According to one embodiment of the present invention, after the moving module drives the first tube body 40 to move to the target container, the peristaltic pump 60 is started to extract the sediment through the first tube body 40 and the second tube body 50; According to one embodiment of the present invention, after the moving module drives the first tube body 40 to move to the target container, the end of the first tube body 40 is placed in the container, and the peristaltic pump 60 is started during the downward movement of the first tube body 40 relative to the container, so that the first tube body 40 can be sucked while descending, thereby solving the problem of suction disturbance.

[0035] In this embodiment, the peristaltic pump adopts a 6-channel metering peristaltic pump, the number of the first tube body 40 and the second tube body 50 for suction are 6 respectively, and the control panel is a touch-type human-computer interaction; the tube body for suction is made of polytetrafluoroethylene; the container is a glass measuring cylinder, and when processing, the second mobile module 30 works at most 6 channels at a time, can be executed continuously for up to 6 times at a time, and can process up to 36 groups of samples at a time.

[0036] After starting, the equipment will perform treatments from left to right in sequence. During treatment, the first tube body 40 automatically moves horizontally to the top of the to-be-determined treatment area, stops and stabilizes, and then automatically descends vertically. At this time, the liquid suction peristaltic pump starts, and the descending speed is adjustable. The liquid suction speed is adjustable, generally in a fixed mode (according to the standard). To ensure the solid sediment at the bottom of the pre-treatment liquid, the first tube body 40 descends while sucking liquid to avoid disturbance.

[0037] Embodiment 2 The difference between this embodiment and embodiment 1 is that, see the attached Figure 6 , 7 As shown, the movable frame 80 is provided with a connecting assembly 90, and the movable frame 80 is provided with two oppositely arranged first plate bodies 91, the first plate body 91 is vertically arranged, and at least one horizontally arranged connecting shaft 92 is provided between the two first plate bodies 91, and the two ends of the connecting shaft 92 are respectively connected to the first plate bodies 91, and the connection method can be a rotating connection, and the connecting shaft 92 is arrayed with fourth holes 94 that pass through its shaft body, and the axis of the fourth hole body 94 is perpendicular to the axis of the connecting shaft 92, and the two ends of the connecting shaft 92 are provided with second plates 93 perpendicular to its axis, and the second plate body 93 is arranged between the two first plate bodies 91, and the second plate body 93 and the connecting shaft 92 are fixedly connected or adjustable and movable. One end of the two second plate bodies 93 is connected to the connecting shaft 92, and the other end is connected by a rod body to form a frame structure, and by applying force to the frame structure, the frame structure can drive the connecting shaft 92 to rotate relative to the first plate body 91.

[0038] The first plate body 91 is capable of controlling the installation height of the connecting shaft 92, and the second tube body 50 for suction can pass through the fourth hole body 94 on the connecting shaft 92 and be connected to the interface of the peristaltic pump 60 respectively, so as to avoid the confusion of multiple second tube bodies 50. In addition, the frame structure formed by the second plate body 93 can rotate and pull the relative position height of the second tube body 50 to avoid pipeline confusion on the one hand, and on the other hand, the swinging frame structure can relatively cause the second tube body 50 to be stretched so as to cause the internal diameter of the stretched second tube body 50 to be relatively slightly deformed, and the internal circulation flow rate is relatively changed by means of slight deformation of the internal diameter to achieve the purpose of cleaning the inner wall of the internal pipeline with different flow rates. Generally, this operation is performed during the non-formal sampling process of the sample.

[0039] The embodiments and / or implementation methods described above are only used to illustrate the preferred embodiments and / or implementation methods for realizing the technology of the present invention, and are not intended to limit the implementation methods of the technology of the present invention in any form. Any technical personnel in this field may make slight changes or modifications to other equivalent embodiments without departing from the scope of the technical means disclosed in the content of the present invention, but they should still be regarded as technologies or embodiments that are essentially the same as the present invention. This article uses specific examples to illustrate the principles and implementation methods of this application. The description of the above embodiments is only used to help understand the method and its core ideas of this application. The above is only the preferred implementation method of this application. It should be pointed out that due to the limitations of textual expression and the objective existence of infinite specific structures, ordinary technicians in this technical field can make several improvements, modifications or changes without departing from the principles of this application, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the concept and technical solution of the invention to other occasions without improvement, should be regarded as the scope of protection of this application.

Claims

1. A high-throughput phytoplankton cell counting pretreatment device, comprising a first substrate (10) for placing a container, a second substrate (12) being arranged on the side of the first substrate (10), and a movable module being arranged on the second substrate (12) capable of displacement relative to the container, characterized in that: The movable module is connected to a first tube body (40), and the movable module is connected to a peristaltic pump (60) via a second tube body (50). The first tube body (40) and the second tube body (50) are arranged in communication.

2. The high-throughput phytoplankton cell counting pre-treatment device according to claim 1, characterized in that: The movable module comprises a first movable module (20) capable of moving in a horizontal direction, the first movable module (20) being arranged on a second base (12), a second movable module (30) capable of moving in a vertical direction being arranged on the first movable module (20), and a clamping assembly (70) being arranged on the second movable module (30).

3. The high-throughput phytoplankton cell counting pre-treatment device according to claim 1, characterized in that: The clamping assembly (70) comprises a first clamping plate (71) connected to the second movable module (30); the first clamping plate (71) is provided with a second clamping plate (72) and a third clamping plate (73) which are arranged horizontally at the upper and lower ends thereof; the second clamping plate (72) and the third clamping plate (73) are provided with a third hole (76) on the surface thereof; the third hole (76) is capable of allowing the first tube (40) or the second tube (50) to enter and exit.

4. The high-throughput phytoplankton cell counting pre-treatment device according to claim 3, characterized in that: The second clamping plate (72) and the third clamping plate (73) are both provided with a first strip-shaped groove body (75), and the third through hole (76) is provided on the first groove body (75). One end of the first groove body (75) is connected to the side of the second clamping plate (72) and the third clamping plate (73), and the side of the second clamping plate (72) and the third clamping plate (73) is provided with a second hole body (74) connected to the first groove body (75).

5. The high-throughput phytoplankton cell counting pre-treatment device according to claim 1, characterized in that: The container comprises a first container (11) and a second container (14); the first base body (10) is provided with a first hole (13) capable of accommodating the first container (11); the hole wall of the first hole (13) is provided with a circular groove having a diameter larger than that of the first hole (13).

6. The high-throughput phytoplankton cell counting pre-treatment device according to claim 1, characterized in that: The peristaltic pump (60) is arranged on a moving frame (80), and a positioning block or a moving wheel is arranged at the bottom of the moving frame (80).

7. The high-throughput phytoplankton cell counting pre-treatment device according to claim 1 or 6, characterized in that: The level of the inlet end of the peristaltic pump (60) is higher than the level of the upper end of the container.

8. The high-throughput phytoplankton cell counting pre-treatment device according to claim 1, characterized in that: The first base (10) is arranged horizontally, the second base (12) is arranged vertically on the side of the first base (10), and the movable module is arranged above the container on the first base (10).

9. A sample processing method using the high-throughput phytoplankton cell counting pre-processing device according to any one of claims 1 to 8, characterized in that: The sample processing method comprises the following steps: Step 1: Place the sample in a container, add the first test solution, and let it stand; Step 2: After the standing time is over, the supernatant and the bottom sediment in the container are obtained, the supernatant on the top of the container is removed by suction, and the sediment in the container is sucked out by a peristaltic pump (60).

10. The sample processing method using the high-throughput phytoplankton cell counting pre-processing device according to claim 9, characterized in that: The first test solution is Lugo test solution; Preferably, after the moving module drives the first tube body (40) to move to the target container, the peristaltic pump (60) is started to extract the sediment through the first tube body (40) and the second tube body (50); Preferably, the moving module drives the first tube body (40) to move to the target container and then places the end of the first tube body (40) into the container, and starts the peristaltic pump (60) when the first tube body (40) moves downward relative to the container.

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